Digital Commons Network^™

Novel Copper Loaded Core-Shell Silica Nanoparticles With Improved Copper Bioavailability Synthesis, Characterization And Study Of Antibacterial Properties, Pavithra Maniprasad

Electronic Theses and Dissertations

A novel core-shell silica based antimicrobial nanoparticle was synthesized. The Stöber silica shell has been engineered to accommodate copper. Synthesis of the core-shell Cu-silica nanoparticle (C-S CuSiNP) involves preparation of base-hydrolyzed Stöber silica “seed” particles first, followed by the acid-catalyzed seeded growth of the Cu-silica shell layer around the core. Scanning electron microscopy and transmission electron microscopy showed monodispersed, spherical shaped nanoparticles with smooth surface morphology. Characterization of particle size distribution in solution by the Dynamic Light Scattering (DLS) technique was fairly consistent with the electron microscopy results. Loading of Cu to nanoparticles was confirmed by the SEM-Energy Dispersive X-Ray …

A Solid Phase Assay For Topoisomerase I Interfacial Poisons And Catalytic Inhibitors, Vidusha Cyril

Electronic Theses and Dissertations

We report a mechanism based screening technique to rapidly identify eukaryotic topoisomerase I targeting agents. The method is based on genetic tagging of topoisomerase I to immobilize the enzyme on a solid surface in a microtiter well format. DNA is added to the wells and retained DNA is detected by Picogreen fluorescence. Compounds that result in an increase in Picogreen staining represent potential topoisomerase interfacial poisons while those that reduce fluorescence report catalytic inhibitors; therefore, the solid phase assay represents a „bimodal‟ readout that reveals mechanisms of action. The method has been demonstrated to work with known interfacial poisons and …

Organizational Support, Organizational Citizenship Behavior, And Perceived Performance Analysis Of Crime Scene Investigation Units Of Turkish National Police, Aykut Tongur

Electronic Theses and Dissertations

Policing is more difficult than ever before in today’s world since types of crime and criminal profiles change as a result of technological development and globalization. Police organizations should review their organizational and operational strategies to improve the fight against contemporary crimes and criminals. Behaviors and performance of police officers are very important in fighting crime. In this struggle, especially today, officers should exhibit organizational citizenship behaviors and perform better. One of the most important factors affecting these two concepts in organizations is organizational support. The literature stresses the social exchange cycle and reciprocity rules in the relationships of organizations …

Identification Of Factors Influencing The Commission Of Burglaries, Mustafa Donmez

Electronic Theses and Dissertations

As in many countries, burglary is a very serious crime in Turkey. Scientific methods and techniques are needed to solve complex burglary cases. This study is completed in the Bursa Police context since they classified many crime data conducive to scientific studies under a project called BEMTAP. The main purpose of this study is to examine the factors influencing the commission of burglaries, using an epid-criminological perspective. It can be argued that factors leading to the commission of a crime are important for formulating preventive strategies in the community. In this study, the contributing factors are categorized into three main …

Molecular Analyses Of Changes Induced In The Microbial Populations Of Murine Colon After As(Iii) Exposure, Rishu Dheer

Electronic Theses and Dissertations

The gut microbiota is essential for mammalian health and metabolism. Thus identifying factors that influence the host microbiota is key to understanding the dynamic interplay between the host and its microbiota. Recent studies have shown the effect of chronic exposures of trivalent arsenic [As(III)] in environmentally relevant concentrations on host physiology, however, little is known of how it impacts the gut microbiota.

This study examined the hypothesis that environmentally relevant concentrations of As(III) in drinking water will directly affect the murine colon microbial composition and physiology. The colon microbial communities from 10 and 250 ppb of As(III) exposed mice were …

An Evaluation Study Of The Effectiveness Of Using A Reaction-Based Process For Hydrazine Waste Remediation, Cristina M. Oropeza

Electronic Theses and Dissertations

Hydrazine (HZ) and monomethylhydrazine (MMH) are used extensively as hypergolic propellants at Kennedy Space Center. These highly reactive fuels are considered highly toxic, and potentially carcinogenic. Consequently, the transport, handling, and disposal of hydrazines is strictly regulated to protect personnel and the environment. Currently, KSC generates large volumes of hydrazine-laden wastewater for disposal. This waste is contained and shipped on public highways for subsequent disposal by incineration presenting a potentially catastrophic threat to the environment and the general public in the event of an accidental release. Other existing remediation methods include oxidative and reductive pathways as well as biodegradation in …

Gore's Science The Kairos Of An Inconvenient Truth And The Implications For Science Writing, Carolyn M. Glasshoff

Electronic Theses and Dissertations

Modern Americans are exposed to scientific and technical information on a daily basis that urges them to react as well as learn about new ideas. The popular science writing that circulates this information must be portrayed in a way that makes it easy for lay people to understand complicated ideas while at the same time remaining complex enough to convince readers that the information is reliable, accurate, and worth learning. In making decisions about how to accomplish this balancing act, science writers make decisions that influence the audience's opinion about new scientific ideas, how easily the audience will accept or …

Are Mbas And Master's Degrees Worth It? A Discussion Of Wages, Job Security And Business Cycles, Claire Foley

Electronic Theses and Dissertations

Since the 1970s, recessions have become more frequent and severe, causing major changes to the labor market and the importance of education. This study focuses on the problems for employees with master's degrees and MBAs, and asks three questions: 1) Do employees with graduate degrees have higher earnings than bachelor's degree recipients, 2) Do they experience greater job security as a result of their additional education, and 3) Is graduate school a way to avoid unemployment during a recession? Ordinal logistic regression analysis is conducted and finds that master's degrees and MBAs experience very different outcomes, with much higher returns …

Molecular Genetic Basis Of Opposite Gene By Environment Interactions In Reading Disability And Attention Deficit/Hyperactivity Disorder, Jennifer Rosenberg

Electronic Theses and Dissertations

The goal of this study is to better understand the genetic basis of Reading Disability (RD) and Attention Deficit Hyperactivity Disorder (ADHD) by examining molecular G x E interactions with parental education for each disorder. Research indicates that despite sharing genetic risk factors, RD and ADHD are influenced by different types of G x E interactions with parental education - a diathesis stress interaction in the case of ADHD and a bioecological interaction in RD. In order to resolve this apparent paradox, we conducted a preliminary study using behavioral genetic methods to test for G x E interactions in RD …

Family Involvement Within Assisted Living: Care-Receivers' And Caregivers' Roles And Relationships, Rachel Vineet Solomon

Electronic Theses and Dissertations

Family systems providing informal care and support to their aging relatives, who were residing in assisted living systems and receiving formal care were central to this study. A broader understanding of family involvement, with respect to exploring both care-receiver and caregiver roles and relationships, within the regulatory and environmental context of assisted living was sought through a qualitative research process. This study employed a phenomenological approach to conduct in-depth interview sessions with eight pairs of participants, comprising elderly residents in five assisted living facilities and their respective caregiving family members, typically a daughter or son and, in one case, a …

Memory Access Patterns For Cellular Automata Using Gpgpus, James Michael Balasalle

Electronic Theses and Dissertations

Today's graphical processing units have hundreds of individual processing cores that can be used for general purpose computation of mathematical and scientific problems. Due to their hardware architecture, these devices are especially effective when solving problems that exhibit a high degree of spatial locality. Cellular automata use small, local neighborhoods to determine successive states of individual elements and therefore, provide an excellent opportunity for the application of general purpose GPU computing. However, the GPU presents a challenging environment because it lacks many of the features of traditional CPUs, such as automatic, on-chip caching of data. To fully realize the potential …

Is Religion "Just" Supernatural Agency, Social Support, Or Meaning?, A. Taylor Newton

Electronic Theses and Dissertations

There is longstanding tension in the study of religion between those who believe religion can be reduced to general psychosocial processes and those who think that religion is somehow unique. One way to test these two possibilities is to compare religious versions of mechanisms to nonreligious versions. If religion is somehow unique, then the religious versions should explain variance in outcomes that the nonreligious versions do not. Three studies confirmed religion's independent predictive power. Exposure to a religious supernatural agent reduced cheating more than exposure to a nonreligious supernatural agent (Study 1), receiving religious social support during a stressful task …

Plasma Membrane Dynamics In The Drosophila Embryo, Amelia E. Zommer

Electronic Theses and Dissertations

Convergent extension is a highly conserved process among mammals, in which the tissue narrows in one axis, and extends across another. Tissue elongation is directed by the regulation of cell interface behaviors, which guides cell intercalation and rosette formation. Rosette formation occurs through the contraction of vertically oriented cell interfaces, and the subsequent elongation of new horizontal interfaces. It has been shown that actomyosin-generated tension functions to direct rosette formation. In this thesis, I have tested the function of regulators of F-actin networks, as well as endocytic and exocytic mechanisms, to identify new components that control interface behaviors and cell …

Parented Emotion: A Study Of Adult-Children's Emotion Socialization And Family Communication Patterns, Joseph Grant Velasco

Electronic Theses and Dissertations

Using the General Theory of Family Communication Patterns (FCPT) and Emotion Regulation Theory (ERT) of Meta-emotion, Parenting, and Child Outcomes, this dissertation sought to investigate adults' emotion socialization in their family of origin through an examination of multiple family schemata. Furthermore, the interactions of parental meta-emotions, family communication patterns, and family communication environments were investigated. Two surveys were used in this study. First, 228 adult-children across two universities in the western United States responded to items retrospectively describing the family communication patterns and family communication environments within their home of origin during their adolescence. Additionally, adult-children described the meta-emotion philosophies …

Micrornas 9a, 9b, 9c And 315 Regulate Expression Of A Reporter For The Neuronal Microtubule-Associated Protein Futsch/Map1b, Leslie M. Rozeboom

Electronic Theses and Dissertations

Fragile X syndrome (FXS) is the most common form of inherited mental retardation in humans. FXS is caused by loss of the Fragile X Mental Retardation Protein (FMRP), an important regulator of neuronal mRNA translation. Patients with FXS display cognitive deficits including memory problems. Protein synthesis-dependent long-term changes in synaptic plasticity are involved in the establishment and maintenance of long-term memory. One prevalent theory of FXS pathology predicts that FMRP is required to negatively regulate the translation of important mRNAs at the synapse. We are investigating microRNAs (miRNAs) as a potential regulator of synaptic FMRP-regulated mRNAs that have previously been …

Margaret Cavendish's Exploration Of Consciousness In Her Writings, Cynthia Lynne Rogan De Ramirez

Electronic Theses and Dissertations

Writing at a time when women had few property rights, were given scarce educational opportunities, and were viewed as incorrigibly irrational, the largely autodidactic English intellectual Margaret Cavendish is fascinated by knowledge and how to secure for herself a place in the micro- as well as macrocosmic community of letters. In particular, Cavendish holds an abiding interest in what we now call "consciousness" which she attributes to every piece of matter. Throughout the universe, the three aspects of matter--inanimate, sensate, and rational--are omnipresent. While throughout all of Cavendish's eclectic literary creation, consciousness is the unifying principle. Her exploration of consciousness …

Simulation, Application, And Resilience Of An Organic Neuromorphic Architecture, Made With Organic Bistable Devices And Organic Field Effect Transistors, Robert A. Nawrocki

Electronic Theses and Dissertations

This thesis presents work done simulating a type of organic neuromorphic architecture, modeled after Artificial Neural Network, and termed Synthetic Neural Network, or SNN. The first major contribution of this thesis is development of a single-transistor-single-organic-bistable-device-per-input circuit that approximates behavior of an artificial neuron. The efficacy of this design is validated by comparing the behavior of a single synthetic neuron to that of an artificial neuron as well as two examples involving a network of synthetic neurons. The analysis utilizes electrical characteristics of polymer electronic elements, namely Organic Bistable Device and Organic Field Effect Transistor, created in the laboratory at …

Label-Free Biochemical Recognition Using Mems Resonators For Microarray Technology, Babak Tousifar

Electronic Theses and Dissertations

Highly sensitive biosensors capable of detecting and characterizing smallest quantities of cellular and molecular targets are needed in pharmaceutical and medical diagnostics industries. In this work, the importance of biological target recognition specifically proteins through microarray technologies has been discussed and the most successful tools and techniques have been studied. Moreover, a thermally actuated Micro Electro-Mechanical Systems (MEMS) resonator has been demonstrated and fabricated in this work as an accurate, reliable and low cost biotechnology tool. As a proof of concept, amine to epoxide coupling of octadecylamine to functionalized silicon dioxide surface have been shown through resonator frequency monitoring. The …

The Impact Of Shyness On Loneliness, Social Anxiety, And School Liking In Late Childhood, Charity M. Walker

Electronic Theses and Dissertations

Shyness is associated with several emotional, social, and academic problems. While there are multiple difficulties that often accompany shyness, there appear to be some factors that can moderate negative effects of shyness. Research has demonstrated that certain parenting factors affect the adjustment of shy children in early childhood, but there is minimal research illuminating the effect of parenting factors in older age groups. The first purpose of this study was to examine relationships between shyness and loneliness, social anxiety, and school liking. The second purpose was to investigate whether the quality of the relationship between a parent and a 10- …

Genetic Polymorphisms And The Treatment Outcome In Standard-Risk Pediatric Acute Lymphoblastic Leukemia, Qi Wei

Electronic Theses and Dissertations

Acute lymphoblastic leukemia (ALL) is the most common childhood cancer and accounts for 30 - 35% of all cancers in children. Significant improvement in the treatment of pediatric ALL has been achieved in recent years. Only 50 years ago, the disease was uniformly fatal with an Overall Survival (OS) rate < 5%. Modern-day, multi-drug chemotherapy is associated with an overall survival rate over 80%. Standard-risk ALL comprises the majority of ALL with an overall survival approaching 90%. Despite this success, children who relapse from this disease accounts for the majority of cancer-related deaths in children. The backbone of treatment protocols have incorporated somatic but not host genetic features in the treatment regimens. The current study examined 12 genetic polymorphisms affecting the pharmacodynamics of antileukemic drugs in an attempt to identify biologic markers related to the risk of disease relapse.

In the current research program, 125 standard-risk ALL patients who were treated at The Children's Hospital were enrolled in a retrospective study. Statistical analysis was performed to evaluate the association between genetic polymorphisms and risk of disease relapse in this study cohort. The GSTM1 null genotype was associated with a …

Rebuilding Character: The Practices Of Positive Youth Development Teachers In A Youth Correctional Setting, Edward Anthony Cope

Electronic Theses and Dissertations

There is a worldwide call for alternatives to current systems focused on restorative justice. The United States currently has a process but no plan in implementing effective programs for rehabilitating youth offenders. For many years, the prevailing idea in youth corrections has been that nothing works as studies continued to have difficulty pinpointing effective programs. Yet, over the last several decades, prison populations have risen dramatically, as have penal housing costs.

Positive Youth Development (PYD) offers a different framework focused on developing youth assets and promoting positive adolescent development. Derived from research in behavioral sciences, PYD programs promote the development …

Functional Activation Of Cartilaginous Fish Melanocortin Receptors, Christina Lee Reinick

Electronic Theses and Dissertations

The evolution of the melanocortin receptor (MCR) gene family has been dictated by two genome duplication events (2R hypothesis). The gnathostomes are thus predicted to possess a minimum of four MCR genes. Previous studies on cartilaginous fish have shown evidence for the presence of only three MCRs. The purpose of this thesis is to functionally express the MC5R from the spiny dogfish (Squalus acanthias) and the putative MC2R and MC3R from the elephant shark (Callorhinchus milii); these receptors have not yet been fully characterized. In this study, SacMC5R was able to be expressed in CHO cells …

Hepatitis C Virus-Induced Hepatocellular Carcinoma: Cancer Stem Cell And Gene Therapy, Heba Samir Esmaeil Allam

Electronic Theses and Dissertations

Hepatitis C virus (HCV) is an important human pathogen that causes chronic hepatitis cirrhosis, steatosis and hepatocellular carcinoma (HCC) worldwide. Although current FDA-approved drugs are partially effective in the treatment of HCV, methods for cure and vaccine against the virus are still awaited. My thesis work presented here is focused on finding mechanism (s) of HCV-induced hepatocarcinogenesis and devising novel means to combat the HCV infection.

Herein, we have shown that long-term HCV expression results in the acquisition of cancer stem-like cells (CSCs) traits in liver derived cell lines. These traits include enhanced expression of putative stem cell markers DCAMKL-1, …

Porting Transmedia Storytelling To Journalism, Kevin Timothy Moloney

Electronic Theses and Dissertations

This thesis examines how the methods of transmedia storytelling emerging in the entertainment industry might be used in a journalism context. Journalism is facing many crises, not the least of which is a loss of readership and perceived relevance to its public. Presented with an ever-expanding array of media with which to interact, the public is more difficult to attract to a socially relevant issue or a politically important story. Faced with similar issues, the entertainment industry has developed a means to engage with fans in a way that draws them across multiple media platforms, better captures their imagination and …

Kids And Kingdom: The Precarious Presence Of Children In The Synoptic Gospels, Arthur James Murphy

Electronic Theses and Dissertations

At a time when institutions of faith are increasingly confronted with scandals that have brought the vulnerability of young children to the fore of religious consciousness, some scholars have pointed to Jesus' special concern and affection for children in the Gospels. Scholars are struck by how often the authors place Jesus in relation to children: healing them, blessing them, and challenging their marginal status by characterizing them as models for understanding the kingdom of God proclaimed by Jesus. If the authors had concluded their stories of Jesus' eschatological gathering with the blessing of the children, then their characterizations of children …

The Social Determinants Of Hiv/Aids In Cambodia: Is It Too Early To Call Micro Medical Efforts A Success?, Matthew Aaron Sherwood

Electronic Theses and Dissertations

Cambodia, a least developed country (LDC) with a recent history of civil war and genocide, has been widely considered to be a success story when it comes to the fight against HIV/AIDS. Within a few years of the first HIV positive blood test in the kingdom in 1991, it quickly became the country with the highest prevalence in the region, with estimates of as many as 4% of the population being infected at one point.

In recent years, Cambodia's HIV/AIDS infection rates have plummeted to below 1%, with most of the credit being given to the implementation of micro-medical measures, …

Products Of Their Environment? Nuclear Proliferation And The Emerging Multipolar International System, Jonathan David Moore

Electronic Theses and Dissertations

The world is shifting from a unipolar system following the end of the Cold War to a multipolar system that is ushered in by "the rise of the rest." This change in the global structure has led some analysts to predict an increase in nuclear weapons proliferation caused by increased uncertainty and a decrease in alliances and security assurances. Nuclear proliferation, however, will not increase because these types of predictions are founded upon realist assumptions that inaccurately predict the characteristics of the emerging multipolar system as well as inaccurately understanding calculations of states with regard to nuclear weapons programs. I …

The Sheddase Activity Of Adam10/Adam17 On Cxcl16 Increases Proliferation And Survival Of Colorectal Cancer Cells, Tamu C. Talton

Electronic Theses and Dissertations

CXCL16 is an interferon-inducible chemokine of the CXC-subfamily and functions as an adhesion molecule, when membrane bound, and a chemoattractant when soluble. Upregulation of cell associated CXCL16 (cCXCL16) in colorectal cancer is associated with increased tumor infiltrating lymphocytes and good prognosis. ADAM10 and ADAM17 are metalloproteinases responsible for cleaving CXCL16, releasing soluble CXCL16 (sCXCL16) and contributing to proliferation and migration of mesangial cells, in kidney inflammatory disease. We hypothesize that cCXCL16 is a substrate for ADAM10 and ADAM17 cleavage in colorectal cancer, releasing sCXCL16, which mediates cell proliferation. To this end, we first identified CXCL16 in the human colon carcinoma …

Providing Culturally Appropriate Services To Immigrant Children And Families Involved With The Allegheny County Department Of Human Services, Turan Jafarova

Electronic Theses and Dissertations

In the last decade, the immigrant population in Allegheny County has doubled and currently accounts for approximately seven percent of the general population. This number, despite being low, especially compared to other counties and states, is very diverse from an ethnic point view and includes immigrant and refugees from Iraq, Bhutan, Burma, Burundi, Nepal, Somalia, numerous Latin American and other countries. Such an ethnically diverse population presents new opportunities for Allegheny County, the population of which has been declining and aging, at the same time posing various challenges to local human service organizations.

This research studies the experience of human …

The Effects Of Fluency-Based Instruction On Skill Acquisition In Children Diagnosed With Landau Kleffner Syndrome, Melissa Kuban Ramirez

Electronic Theses and Dissertations

Landau Kleffner Syndrome, or acquired epileptic aphasia, is an epileptic syndrome involving a neurological impairment related to the appearance of paroxysmal (i.e., sudden intense) electroencephalograph (EEG) activity (Pearl, Carrazana & Holmes, 2001). Landau Kleffner syndrome results from an epileptogenic lesion arising in the speech cortex during a critical period of development, which may interfere with the establishment of satisfactory and functional circuits for normal language function (Morrell et al., 1995). LKS is a complex and severe syndrome that affects all aspects of a child's life, including communication, socialization, and the everyday ability to function within the environment.

An option for …