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Group Sandtray Therapy With Adult Survivors Of Childhood Sexual Assault: A Single-Case Research Design, Jana Elaine Scott Jan 2021

Group Sandtray Therapy With Adult Survivors Of Childhood Sexual Assault: A Single-Case Research Design, Jana Elaine Scott

Electronic Theses and Dissertations

Childhood sexual assault (CSA) is a growing problem in our society. A child who experiences a sexual assault not only deals with the immediate trauma surrounding this event, but also the aftermath of this event as they progress into adulthood. Trauma and sexual trauma is an umbrella term that incorporates all of the various implications of experiencing a negative event. In the instance of trauma, neurobiological implications have been well researched and established. This knowledge highlights the need for counseling techniques to be developed and studied that target those who have experienced CSA. The aim of this single-case research design …


Enzyme-Initiated Admicellar Polymerization, Elaheh Shakeri Jan 2021

Enzyme-Initiated Admicellar Polymerization, Elaheh Shakeri

Electronic Theses and Dissertations

Admicellar polymerization was developed in the 1980s as an economical method for modifying various surfaces. In admicellar polymerization, a low monomer to initiator (M/I) ratio was traditionally required to overcome this limitation and achieve high polymer conversion when oxygen was present in the headspace and dissolved in the reaction medium. To develop an environmentally friendly, cost-efficient, and feasible deoxygenation and initiation technique, we studied the use of glucose oxidase (GOx) in admicellar polymerization. In this method, GOx acts as the catalyst in the oxidation of ?-D-glucose, while consuming oxygen and generating hydrogen peroxide and D-glucono-?-lactone. There are many advantages of …


Reducing Disparites In Act Results According To Racial Identity: An Applied Research Study Into The Weact Program In Tinlake High School, Marcus Stewart Jan 2021

Reducing Disparites In Act Results According To Racial Identity: An Applied Research Study Into The Weact Program In Tinlake High School, Marcus Stewart

Electronic Theses and Dissertations

ABSTRACT The ACT provides opportunities for students of various socioeconomic backgrounds, racial identities, and family structures. Throughout the nation, there is a disparity that involves Black students scoring less than White students on the test by a margin of five points. The applied mixed methods research aimed to identify elements to help decrease the gap in student scores. The combined efforts of all the elements, tutorials, boot camps, ACT prep classes, and providing ACT resources in daily instruction formed the WeACT program. The students of Tinlake High School were exposed to this pilot program to assist in their ACT growth. …


Just Southern Food: Food Justice For The Mississippi Delta, Christian Tabor Owen Jan 2021

Just Southern Food: Food Justice For The Mississippi Delta, Christian Tabor Owen

Electronic Theses and Dissertations

The primary objective of this research is to promote food justice for the Mississippi Delta by investigating facts about the intersections of extreme poverty, food insecurity, and chronic illness in the Mississippi Delta. By exploring relevant literature and highlighting current initiatives, this work looks at the semantics of food justice and related terms, discusses challenges unique to the Mississippi Delta, and broadly characterizes public health models with the greatest potential for food justice advancement in this region. Pivotal to interpreting food justice not only for the Mississippi Delta or the Global South, but for any community, is a clear understanding …


Evaluating Canola Genotypes For Growth And Yield Under Different Environmental Conditions In South Dakota, Unius Arinaitwe Jan 2021

Evaluating Canola Genotypes For Growth And Yield Under Different Environmental Conditions In South Dakota, Unius Arinaitwe

Electronic Theses and Dissertations

Canola (Brassica napus L) and carinata (Brassica carinata A. Braun) are potential oilseed crops for diversifying cropping systems and expanding into marginal lands impacted by saline and sodic soils in South Dakota (SD). However, genotypes that are high yielding, with high agronomic adaptability and stability over diverse environments, and salt tolerant have not been selected. One field experiment was conducted at two environments (Brookings - eastern SD and Pierre - western SD) from 2019 to 2020 to evaluate genotypes for growth and yield stability. Three greenhouse experiments were conducted to evaluate genotypes for salt tolerance in …


Developing Whey Protein Encapsulated Probiotics And Evaluating Environmental Listeria Using Whole-Genome Sequencing, Shayanti Minj Jan 2021

Developing Whey Protein Encapsulated Probiotics And Evaluating Environmental Listeria Using Whole-Genome Sequencing, Shayanti Minj

Electronic Theses and Dissertations

In this study, a novel value-added dairy-based health formulation was developed using whey protein hydrolysate and probiotic organisms. In the first part of the study, different forms of whey proteins, concentrate WPC80, isolate WPI90, and hydrolysates WPH10, WPH15 and WPH20 were screened for bioactivities (antimicrobial, antioxidant, and antihypertensive activity). Hydrolysate WPH10, exhibiting the highest bioactivities was conjugated with maltodextrin. A batch of 2L conjugated solution was spray dried in a Niro drier with an inlet and outlet temperature of 200⁰C and 90⁰C, and alternatively, freeze dried at −80 °C under 50 mTorr vacuum. The bioactivities of the conjugated samples were …


An Integrative Analysis On The Role Of Notch1 And Notch3 In Musculoskeletal Disease, Haydee Magdalena Torres Jan 2021

An Integrative Analysis On The Role Of Notch1 And Notch3 In Musculoskeletal Disease, Haydee Magdalena Torres

Electronic Theses and Dissertations

Notch signaling is an evolutionarily conserved signaling pathway involved in cell fate decisions and cell renewal of multiple systems, including skeletal and muscle homeostasis. As the multifunctional nuclear complex of Notch1 is essential for its pathological function, identifying these interacting partners represents a potentially broad range of druggable vulnerabilities. We have completed a BioID proteomics study and found a large set of nuclear proteins associated with NOTCH1, including transcriptional regulators and epigenetic modulators, of which Histone Deacetylase 1 (HDAC1) is a promising interacting partner of Notch1. Our Cytoscape analysis predicted HDAC1 to be a top critical node in an activated …


Bio-Inspired Materials For Electrochemical Sensors, Matthew Joseph Hummel Jan 2021

Bio-Inspired Materials For Electrochemical Sensors, Matthew Joseph Hummel

Electronic Theses and Dissertations

Electrochemical biosensors are a rapidly growing research area that has greatly improved its specificity, accuracy, and precision in the detection of biomolecules in contemporary literature and industry alike. Typically, these systems exist in a three-electrode conformation with a working electrode functioning as the anode, a counter electrode functioning as the cathode, and a reference electrode allowing for the control of potential in the system. The method by which these sensors work is through the sharing of electrons via redox reactions with the target molecule and the working electrode or modifications on its surface. By exploiting the function of biomaterials that …


Cold Climate Grape Cultivar`S Physiological And Gene Expression Responses To Low And Freezing Temperatures, Turhan Yilmaz Jan 2021

Cold Climate Grape Cultivar`S Physiological And Gene Expression Responses To Low And Freezing Temperatures, Turhan Yilmaz

Electronic Theses and Dissertations

Grapevine (Vitis Vinifera), widely cultivated in the world and USA, is a significant and valuable fruit crop. After cold climate grapevine cultivars were released by breeding programs in the 1990s, the production of grapes expanded in the Northern cold climate region of the US. The objectives of this study were to test 1) freezing tolerance and chilling fulfillment, 2) the effect of pruning methods on yield and winter survival, and 3) transcriptomic changes in natural and controlled chilling conditions during chilling requirement fulfillment in cold climate grapevine cultivars. Pruning methods, spur (SP), short cane (SC), and spur plus short cane …


Metal-Organic Frameworks As Bacteria Mimicking Delivery Systems For Tuberculosis, Ailin Guo Jan 2021

Metal-Organic Frameworks As Bacteria Mimicking Delivery Systems For Tuberculosis, Ailin Guo

Electronic Theses and Dissertations

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (M.tb), with an estimated 1.5 million deaths and 10 million infections each year. Although TB can be effectively treated with antibiotics, because of the complications and length of treatment, many people fail to complete the treatment which exacerbates the emergence of drug-resistant M. tb strains. The goal of this work is to develop biomimetic particles as host-directed therapy to target the infected macrophages. Two types of metal-organic frameworks (MOFs), MIL-100(Fe) and MIL-88A(Fe) were developed for bacteriamimicking particles. As a proof-of-concept, Mannose was selected as a ligand to target macrophages because …


An Exploration Of The Effects Of An Early Postpartum Intravenous Infusion With Carnosic Acid On Physiological Responses Of Transition Dairy Cows, Tainara Michelotti Jan 2021

An Exploration Of The Effects Of An Early Postpartum Intravenous Infusion With Carnosic Acid On Physiological Responses Of Transition Dairy Cows, Tainara Michelotti

Electronic Theses and Dissertations

The transition period is commonly defined as the last 3 wk before parturition to 3 wk after parturition. It is the most critical stage of the lactation cycle of high-producing dairy cows. The period consists of a complex interplay of metabolic and hormonal adaptations, inflammation, and immune activation. During the transition period, dairy cows commonly face oxidative stress, an underlying factor for dysfunctional immune response and enhanced inflammation, which can further increase the susceptibility of dairy cattle to health disorders and decrease productivity. In the last few decades, there has been an increased interest in studying alternative natural compounds with …


Auxin-Based Herbicide Program And Rhizobia Application For Weed Control And Nodulation Potential In Auxin Tolerant Soybean, Joy Amajioyi Jan 2021

Auxin-Based Herbicide Program And Rhizobia Application For Weed Control And Nodulation Potential In Auxin Tolerant Soybean, Joy Amajioyi

Electronic Theses and Dissertations

Foliar-applied Bradyrhizobia to V4 soybean has been reported to increase yield up to 5%. However, a stand-alone product application may not be practical. Applying with other treatments such as post-emergence herbicide application may be economical but herbicide and/or additives may be deleterious to rhizobial growth. A laboratory study investigated the impact of herbicides (glyphosate and dicamba), additives (an oil to improve absorption and spreading; and AMS used to overcome hard water impacts on glyphosate), and herbicide + additives on bacterial growth. Optical density (OD) measurements at the wavelength of 650 nm assessed solution turbidity, a surrogate measure of bacterial growth. …


Studies On Genetic Characterization Of Agronomic Traits And Disease Resistance In Bread Wheat, Jyotirmoy Halder Jan 2021

Studies On Genetic Characterization Of Agronomic Traits And Disease Resistance In Bread Wheat, Jyotirmoy Halder

Electronic Theses and Dissertations

A steady increase in wheat yield is vital to feed the continuously rising world population. Systematic exploitation of wheat germplasm and a better understanding of the underlying genetic control could be pivotal in accelerating the genetic gain for yield and disease management. Various modern techniques such as genome-wide association study (GWAS), genomic selection (GS), fine mapping, and cloning can expedite wheat improvement and broaden our understanding of the complex wheat genome. In the first objective of this study, we evaluated the Watkins core set of 121 landrace cultivars (LCs) to identify novel sources of resistance against the tan spot, Stagonospora …


Effect Of Chronic Pain On Prospective Memory Performance, Alexander Joseph Kuka Jan 2021

Effect Of Chronic Pain On Prospective Memory Performance, Alexander Joseph Kuka

Electronic Theses and Dissertations

Chronic pain is among the most widespread and disabling conditions worldwide. In the United States, approximately 50 million people suffer from chronic pain, and nearly half that number experience daily chronic pain. Diagnostic testing, treatments, and operations related to chronic pain cost Americans over $600 billion annually. Prospective memory, the process by which people remember to perform an action in the future after a delay, appears to be affected by the experience of pain, especially when a prospective memory task is more cognitively demanding. While self-report studies of individuals with chronic pain suggest that pain adversely affects both their retrospective …


Development, Optimization, And In Vitro Characterization Of Different Anesthetic Wound Products, Alia Alshammari Jan 2021

Development, Optimization, And In Vitro Characterization Of Different Anesthetic Wound Products, Alia Alshammari

Electronic Theses and Dissertations

ABSTRACTAcute and chronic wounds can be a significant source of pain. In the past, pain associated with chronic wounds has been perceived as an inevitable complication of wound care. Fortunately, recent approaches in wound care have developed strategies to prevent and reduce pain when carrying out wound care. Local anesthetics have been used to that end. Lidocaine is the most widely used topical anesthetic in wound care. However, literature on topical Lidocaine has focused almost exclusively on the clinical application on intact skin. This dissertation aims to advance our understanding of anesthetic wound product formulation and development. Anesthetic wound ointments …


Development Of Bio-Based Nanocomposites For Biosensor And Indicator Applications In Smart Food Packaging, Abdus Sobhan Jan 2021

Development Of Bio-Based Nanocomposites For Biosensor And Indicator Applications In Smart Food Packaging, Abdus Sobhan

Electronic Theses and Dissertations

Smart food packaging based on biosensors has been attracting more and more interest to the industrial community because of the concerns of food quality and safety. A food packaging with biosensor has a scope to enable real-time monitoring of microbial breakdown products of packaged foods. Furthermore, one of the biggest challenges in implementing biosensor for smart packaging materials is the development of bio-sensing active materials that can leverage their electrical, thermal, biodegradable and other functional properties. In this regard, nanocellulose-based activated carbon (NAC) nanocomposite was developed using the activated carbon and nanocellulose gel using the casting method with their different …


Profiling Of Fda-Approved And Clinical Trial Drugs Revealed Shared Cytotoxicity And Collateral Sensitivity In Resistant (H69ar) And Non-Resistant (H69) Small Cell Lung Cancer Cells. (Drug Repurposing In Cancer Chemotherapy), Pius Reyderg Agyemang Jan 2021

Profiling Of Fda-Approved And Clinical Trial Drugs Revealed Shared Cytotoxicity And Collateral Sensitivity In Resistant (H69ar) And Non-Resistant (H69) Small Cell Lung Cancer Cells. (Drug Repurposing In Cancer Chemotherapy), Pius Reyderg Agyemang

Electronic Theses and Dissertations

Some cancers are capable of “spitting out” drugs being fed to them, metaphorically speaking, becoming resistant to what were previously effective chemotherapeutics. In small-cell lung cancer (SCLC), an overexpression of a membrane protein (MRP1) and its transport activity can lead to chemotherapy failure. However, this study showed that certain drugs are selectively cytotoxic (exhibit collateral sensitivity) to MRP1-overexpressed SCLC (H69AR) cells. In this study, three drugs (Erlotinib, Pyrimethamine, Fludarabine) were identified to exhibit a dose-dependent collateral sensitivity on H69AR with IC50 values of ~3.5 μM, ~2 μM, and ~20 μM respectively. Halting the transport activity of the MRP1 with 25 …


Free-Living Diazotrophs And The Nitrogen Cycle In Natural Grassland Revealed By Culture Dependent And Independent Approaches, Amrit Koirala Jan 2021

Free-Living Diazotrophs And The Nitrogen Cycle In Natural Grassland Revealed By Culture Dependent And Independent Approaches, Amrit Koirala

Electronic Theses and Dissertations

Biological nitrogen fixation contributes to half of the global supply of nitrogen to the biosphere. It is carried out by a diverse group of prokaryotes called diazotrophs via the nitrogenase enzyme. Nitrogen fixation research is focused on the narrow group of symbiotic diazotrophs, and the vast majority of free-living diazotrophs which contribute significantly to fixed nitrogen are yet to be explored. The goal of this research was to access phylogeny of diazotrophs considering the most up-to-date genomic information and apply that knowledge to understand the diversity of free-living diazotrophs in a natural grassland ecosystem, both by culture dependent and independent …


Association Between Prepregnancy Obesity And Health Behaviors, Adverse Conditions, And Birth Outcomes In South Dakota, Lily Sanderson Jan 2021

Association Between Prepregnancy Obesity And Health Behaviors, Adverse Conditions, And Birth Outcomes In South Dakota, Lily Sanderson

Electronic Theses and Dissertations

Introduction: Prepregnancy obesity is associated with an increased risk for various adverse outcomes for mother and fetus. These adverse associations exist within the realms of preconception health, prenatal health, birth outcomes, and postnatal health.
Methods: This retrospective cohort study used data from the South Dakota Pregnancy Risk Assessment Monitoring System (SD PRAMS) survey, which is an ongoing statebased surveillance system of maternal behaviors, attitudes, and experiences before, during, and shortly after pregnancy. The 2017 and 2018 SD PRAMS sampled a total of 3,805 mothers who were randomly selected from birth certificate records to be representative of all South Dakota women …


Veracious Verdicts: An Expansion Of Cognitive-Experiential Self-Theory In Jury Decision-Making Using Attribution Theory, Jade E. Larson Jan 2021

Veracious Verdicts: An Expansion Of Cognitive-Experiential Self-Theory In Jury Decision-Making Using Attribution Theory, Jade E. Larson

Electronic Theses and Dissertations

As a pillar in our judicial system, the courts utilize almost ten million citizens each year for jury service. As a result, the courts are faced with issues of inconsistency and unpredictability. This study aims to examine some factors that significantly influence jury decision-making by investigating cognitive experiential self-theory (CEST) as a jury decision-making model, unified with attribution theory to better predict verdict outcomes. An online survey was distributed to 121 participants. The respondents were asked to read a civil trial case presentation; they were then randomly divided into two conditions (high and low unrelated detail eyewitness testimony). The testimonies …


Effects Of Harvest Regulations And Post-Release Hooking Mortality On Walleye Populations In South Dakota, Cade Lyon Jan 2021

Effects Of Harvest Regulations And Post-Release Hooking Mortality On Walleye Populations In South Dakota, Cade Lyon

Electronic Theses and Dissertations

Harvest regulations are commonly implemented to manipulate fisheries stocks. By regulating the size and number of fish that are harvested by anglers, managers are able to meet the goals and needs of regions. However, these management actions come with the potential for negative consequences. Overexploitation due to less restrictive harvest regulations can cause collapses in fisheries populations. In addition, indirect consequences such as hooking mortality brought on by length-based regulations can also be detrimental to populations. In this study, I investigated the effects of various harvest regulations on Walleye populations in three western South Dakota irrigation reservoirs: Angostura, Belle Fourche, …


Chronology Of The Fatherland Site, Daniel Shawl Jan 2021

Chronology Of The Fatherland Site, Daniel Shawl

Electronic Theses and Dissertations

A recently discovered mound (Mound E) at the Grand Village of the Natchez Indians (22AD501, the Fatherland Site) dates to the Foster phase of the Mississippian period (1300-1500 CE). This new chronology is stems from analysis conducted on ceramic assemblages from Robert S. Neitzel’s (1965) excavations and ceramic assemblages from Tony Boudreaux’s (2019) excavations at the site. Frequency seriations, non-metric multidimensional scaling, and radiocarbon dates suggest that Mound E was constructed and in use several hundred years prior to European contact at the site. The results of this body of research brings new insight into a portion of history of …


Nature's Converts: Reading The Land And Nature In The Early American Conversion Narrative, 1727-1831, Cullen Rex Brown Jan 2021

Nature's Converts: Reading The Land And Nature In The Early American Conversion Narrative, 1727-1831, Cullen Rex Brown

Electronic Theses and Dissertations

This dissertation examines the connections between cultivating the land and cultivating the soul through select readings of early American conversion narratives composed between 1727-1831. Over a century after British Colonists colonized the shores of New England, members of the United Ministers of Boston championed the efforts of their descendants, who both cultivated the land and the Indigenous and new arrived souls emplaced within the North American landscape. As they told their readers in 1727, their dual cultivating efforts were worthy of universal adulation and imitation. And in 1836, the words of Reverend Nahum Gold echoed those of the United Ministers …


Topics On Applications Of Optimization Theories On Statistical Methodologies, Duc Anh Anh Doan Jan 2021

Topics On Applications Of Optimization Theories On Statistical Methodologies, Duc Anh Anh Doan

Electronic Theses and Dissertations

In this dissertation, We show the results of our researches in statistical sampling, functional optimization, and methodology for partially observed Markov process (POMP) models. In statistical sampling, we introduce a p-generalized smoothing method that enables the Langevin-Monte Carlo method to generate a sample from a log concave distribution weakly smoothing potential function. For our optimization research, we introduce an accelerated inexact gradient (AIG) method. Combining the strengths while mitigating the weakness of its parent methods: gradient descent and Nesterov's accelerated gradient, AIG converges with excellent rates for both convex and non-convex optimization problems for smooth objective functions. Furthermore, we also …


Methylene Blue As Light And Reactive Oxygen Species Sensitive Ligand: Appicability In Hybrid Drug And Material Synthesis, Huy Dao Jan 2021

Methylene Blue As Light And Reactive Oxygen Species Sensitive Ligand: Appicability In Hybrid Drug And Material Synthesis, Huy Dao

Electronic Theses and Dissertations

Methylene blue (MB) with a 10-N-carbamoyl linkage was discovered and developed as a multifunctional far-red (660 nm) photocleavable ligand capable of rendering a series of MB conjugated compounds with off-to-on fluorescence switch properties through the controlled release of MB. The released MB was successfully demonstrated to be an effective photosensitizer, imaging probe, and therapeutic agent. The complete release of MB was achieved with a cumulative light dose of 15 J/cm2. The photosensitivity was demonstrated to be dependent on the type of chemical bond at 10-N of the phenothiazine ring which links MB to a side group. Specifically, the urea bond-based …


Decreasing Discipline Disparities Among African-American Males: An Applied Research Study On The Effects Of Developing Culturally Responsive Teaching Practices Among Teachers And Equipping Students With Added Supports, Reginald Maurice Herrington Jan 2021

Decreasing Discipline Disparities Among African-American Males: An Applied Research Study On The Effects Of Developing Culturally Responsive Teaching Practices Among Teachers And Equipping Students With Added Supports, Reginald Maurice Herrington

Electronic Theses and Dissertations

Schools and school leaders are charged to provide equitable educational opportunities and ensure the success for all students no matter their scholastic ability giving students access to an education that is culturally responsive. Despite this charge, African-American male students receive exclusionary punishment at disparaging rates which contributes to the widening achievement gap between white and black students. The purpose of this applied research study was to decrease the rate at which African-American males are referred to the office. This study analyzes the implementation of two elements: engaging teachers in a book study on culturally responsive teaching strategies and targeted counseling …


Subcutaneous Delivery Of Albumin, Vy Nguyen Jan 2021

Subcutaneous Delivery Of Albumin, Vy Nguyen

Electronic Theses and Dissertations

An increasing number of protein drugs are used in the clinic to treat a broad range of diseases. However, because of their large molecular sizes and high hydrophilicity, protein drugs are not orally bioavailable, which commonly require intravenous, intramuscular or subcutaneous injection. Thermosensitive polymers have been widely investigated as in situ depots for sustained drug release delivered through subcutaneous route.Albumin is the most abundant proteins in bloodstream. It has long half-life (19 days in human) and plays important roles such as acting as carrier for fatty acids, amino acids, waste products and medical compounds. Albumin has been studied for decades …


Equity, Social Justice, And Ethical Responsibility In Higher Education: Voices Of Business Students At A Pervasivley Sectarian University, Van Brooks Poole Jan 2021

Equity, Social Justice, And Ethical Responsibility In Higher Education: Voices Of Business Students At A Pervasivley Sectarian University, Van Brooks Poole

Electronic Theses and Dissertations

This proposed study explores the perceptions and experiences of business students who attend a pervasively sectarian university in the South (MPSU) regarding their perceptions of equity, social justice, and ethical responsibility through their experiences on campus and in their academic program. This study will discuss socio-political influences at the national, state, and local levels and examine existing university policies and practices to identify recommendations for organizational change using Lewin’s unfreezing model as a theoretical framework.


Microbial Community Responses To Salinification: Applications In Climate Change And Extreme Environments, Eric Weingarten Jan 2021

Microbial Community Responses To Salinification: Applications In Climate Change And Extreme Environments, Eric Weingarten

Electronic Theses and Dissertations

Sediment microbial community responses to elevated salinity are important in predicting the effects of sea level rise as well as in understanding those communities that can thrive in extreme environments. In this dissertation, I use natural and field experiments to investigate how salinity influences microbiome structure in coastal wetlands. Through the use of amplicon sequencing, I demonstrate that there are compositional differences between the microbiota of four wetland salinity classes: limnetic, oligohaline, mesohaline, and polyhaline. These results were reciprocated in a microcosm study exposing freshwater sediment to meso- and polyhaline conditions, showing that communities shifted proportionally to the magnitude of …


Epigenetic Regulation In Brevundimonas Subvibrioides And Comparison With The Caulobacter Crescentus Model, Satish Adhikari Jan 2021

Epigenetic Regulation In Brevundimonas Subvibrioides And Comparison With The Caulobacter Crescentus Model, Satish Adhikari

Electronic Theses and Dissertations

Epigenetics is a form of DNA methylation-based gene regulation. The CcrM methyltransferase and transcriptional regulator GcrA, constitute an epigenetic regulatory system well-conserved throughout the Alphaproteobacteria. The regulatory targets of GcrA and CcrM were identified in the Alphaproteobacterium Brevundimonas subvibrioides, and then compared to those of its close relative Caulobacter crescentus. Both these bacteria belong to the same family and inhabit the same environment. Although the regulators themselves are highly conserved, the genes they regulate are vastly different. GcrA directly regulates 204 genes in C. crescentus, and though B. subvibrioides has orthologs to 147 of those genes, only 48 genes retained …