Digital Commons Network^™

The Effect Of Sodium Selenate Biofortification On Plant Yield, Mineral Content, And Antioxidant Capacity Of Culinary Herb Microgreens, Rachel G. Newman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Selenium (Se) biofortification of plants has been suggested as a method for enhancing dietary Se intake. Popular herbs such as basil (Ocimum basilicum), cilantro (Coriandrum sativum), and scallions (Allium fistulosum) are used for enhancing flavors. Microgreens are young seedlings harvested with one to two true leaves and are increasingly popular in the consumer marketplace. In this study, basil, cilantro, and scallion microgreens were treated with various concentrations of Se as sodium selenate in hydroponic conditions to investigate the effect on plant yield, mineral content (selenium, sulfur, sodium, potassium, phosphorus, calcium, magnesium, copper, iron, manganese, zinc, and boron), total phenolic compounds, …

The Effect Of Feed Manufacture Techniques On Pathogen Reduction And Feed Nutrient Value, Timothy Paul Boltz

Graduate Theses, Dissertations, and Problem Reports (ETD)

Experiments were conducted to evaluate how differing feed manufacture techniques, such as varying conditioning times and new equipment, can affect pathogen mitigation, feed manufacture metrics, amino acid digestibility, and enzyme recovery in feed. Feed hygienics are of increasing importance in providing safe feed to animals, and ultimately safe food for consumers. Salmonella has been identified as a major microbial hazard in animal feed that has been linked to animal and human illness. In chapter 2, the effects of standard pelleting and more thermally aggressive techniques were utilized to determine pathogen mitigation potential. Use of antibiotics has decreased in recent years …

Wish4campus: Evaluating College Food Insecurity And Promoting Solutions For Student Wellbeing, Rebecca L. Hagedorn

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: Interest in college food insecurity has increased in previous years, however, little research focuses on the Appalachian and Southeastern regions of the United States resulting in limited understanding of food insecurity’s impact on college students in these regions. Additionally, resources to help food insecure students are often sparse with universities lacking evidence-based programming to implement for student benefit.

Aims: This dissertation aims to (1) investigate the correlates and behavioral consequences of food insecurity on college students at an Appalachian university, (2) expand college food insecurity research to a regional investigation in the Appalachian and Southeastern regions, and (3) develop …

Apple Pomace As A Novel Aid For Western Diet-Induced Nonalcoholic Fatty Liver Disease In Young Female Sprague Dawley Rats, R. Chris Skinner

Graduate Theses, Dissertations, and Problem Reports (ETD)

Apple pomace is a “waste” byproduct of apple processing that causes environmental pollution and is costly to dispose of. Yet, apple pomace is rich in dietary fibers and antioxidants. Analysis of apple pomace’s nutritional profile indicates suitability as a potential dietary treatment for non-alcoholic fatty liver disease (NAFLD) and the more severe non-alcoholic steatohepatitis (NASH). NAFLD is the most prevalent liver disease in the world with prevalence and severity expected to increase in both adults and children. Currently, there is no approved drug treatment for NAFLD and therefore, dietary intervention is the primary treatment. The study objectives were to determine …

West Virginia University’S Changing Mission In The 21st Century, Katlin Swisher

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the midst of the ongoing shifts of the perception of higher education and the public’s question of the role and value of public universities, West Virginia University has simultaneously experienced institutional change through five different presidential administrations in a decade. As seen through WVU’s historical context as the state of West Virginia’s land-grant, flagship, research university, the institution has and continues to face many administrative, policy, and contextual changes as it strives to meet its strategic goals and operationalize its mission. Through document analysis and interviews with key officials, this qualitative, multi-case study seeks to understand how WVU’s mission …

Lead Exposure Of North American Raptors, Vincent A. Slabe

Graduate Theses, Dissertations, and Problem Reports (ETD)

Lead is an anthropogenic threat to terrestrial wildlife, has no physiological benefits, and is considered a neurotoxin. Legislation adopted in many developed countries restricts the uses of lead in consumer and industrial products such as pipe fittings, petrol, paint, and shot for use in waterfowl hunting. However, lead is currently categorized by the United States (US) government as a critical metal and common uses of lead still persist in North America. These include the use of rifle ammunition, shot in upland gamebird hunting, and fishing sinkers. Lead is also a by-product of multiple industrial operations including smelters, coal power plants, …

The Effects Of Obesity, Chronic Stress, And Exercise On Pancreatic Health, Abigail Tice

Graduate Theses, Dissertations, and Problem Reports (ETD)

Obesity is concomitant with hyperglycemic conditions, increased proinflammatory cytokines, and increased oxidative stress. Under hyperglycemia, thioredoxin interacting protein (TXNIP) is increased and causes increased oxidative stress and pancreatic b-cell apoptosis. Chronic stress is associated with increased glucocorticoid levels and a proinflammatory environment. The purpose of this study was to examine the effects of obesity, chronic stress, and exercise on pancreatic health.

Lean Zucker Rats (LZRs) and Obese Zucker Rats (OZRs) were randomly assigned to four groups: 1) sedentary controls (C: n=8 per); 2) unpredictable chronic mild stress (UCMS) (S: n=8); 3) exercise (E: n=8); and 4) combined UCMS and exercise …

Movement In The Classroom: Contingent Factors Underlying Teacher Change, Annie E. Machamer

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Teachers' use of classroom physical activity (PA) has been found to significantly impact students PA participation, time on task, and academic performance. However, teachers have continued to identify barriers affecting delivery related to professional development opportunities, learning environment, and experiences in practice. An understanding of the connection between these experiences and their impact on teacher change is needed.

Purpose: The purpose of this study was to investigate teachers’ perceptions of specific factors that have influenced their continued implementation of classroom PA. This study provides insight into the teacher perceptions, and aims to identify connections among orientation to learning and …

Health Literacy And Family Factors In The Transition To Adult Care In Adolescents With Type I Diabetes, Ellen M. Manegold

Graduate Theses, Dissertations, and Problem Reports (ETD)

Successful management of type 1 diabetes (T1DM) in adolescence involves multiple daily tasks, developmental changes, and the expectation of transition to an adult provider. Health literacy is one variable to consider in the context of transition, as studies have demonstrated the correlation of parental health literacy with health behaviors and outcomes, yet not studied adolescent health literacy in T1DM. Family factors (e.g., management responsibility, diabetes-specific family conflict, parental support) also are important to behavior and health outcomes in adolescents with T1DM. Study aims were to: 1) examine the association of adolescent health literacy to transition readiness and health outcome in …

Following The Leaders: Issue Attention And Agenda Dynamics In Women’S Health Care Policy, Kara Anne Fisher

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation focuses on shifts in issue attention in the policy process and examines policy changes. Describing agenda setting is important not only for understanding congressional behavior in general but also for understanding the institutional context of other political behavior. I focus on the processes of positive feedback to explain periods of dramatic policy changes observed over a long period of time.

The purpose of this study is to examine the changes in the politics of health policy which opened the door to women’s health care as an important feature of health politics. Thus, my research is motivated by three …

Investigation Of Bovine Conceptus Galectins And The Effect Of Bovine Conceptus Secretory Proteins On The Endometrial Epithelial Transcriptome, Heather Lynn Baldwin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Pregnancy loss in cattle is greatest in the first 30 days of gestation. Near d15 of gestation, bovine conceptuses elongate and express LGALS1, an immunosuppressive glycan-binding protein believed to be essential for implantation. However, other members of the bovine galectin family, including LGALS3, LGALS4, LGALS7, LGALS8, LGALS9 and a galectin-13-like gene (LGALS13L), also may be expressed by the elongating conceptus. Therefore, galectin expression in elongating, d16 bovine conceptuses and intercaruncular endometrium as well as elongating, d14 ovine conceptuses and intercaruncular endometrium was assessed by RT-qPCR. Angus heifer and Texel ewes underwent estrous cycle synchronization and either inseminated or not bred. …

A Video Decision Aid To Influence Advance Care Planning, Angela Petry

Graduate Theses, Dissertations, and Problem Reports (ETD)

The best mechanism to achieve successful advance care planning has yet to be identified. A randomized, controlled, experimental study was performed with a sample of 54 men and women who met inclusion criteria and were randomized into the intervention versus the control group in an outpatient clinical setting in rural West Virginia (WV) from December 2018 to March 2019. Participant mean age was 72.4 years. For those who chose to participate (n=42), the intervention group received the usual standard of care, which was a face to face advance care planning (ACP) discussion with the new intervention of a video decision …

Lignocellulosic Biomass Derived Activated Carbon For Energy Storage And Adsorption, Changle Jiang

Graduate Theses, Dissertations, and Problem Reports (ETD)

Lignocellulosic biomass has been converted to hierarchical porous carbon materials which possess macro-, meso- and micro-pores. The natural structure of porous lignocellulosic structure was preserved during activation with further developed porosity by the activation. The activated carbon can be well applied to electrochemical double layer capacitor for transportation storage of ions as well as adsorbent materials for metal ion removal from wastewater.

The first chapter of this dissertation presents an introduction of biomass derived carbon and its applications. In the second chapter, both direct and indirect activation methods using carbon dioxide were adopted in this study. The results show that …

Pain Relief Knowledge, History, And Expectations Among Emergency Dental Patients, Daniel C. Brawley

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction

Dentists frequently treat and manage patients with acute dental pain. Patients often seek care to treat their pain. As a part of that treatment, it is common for providers to use both prescription and non-prescription medications in the management of the patient’s dental pain. This study focused on understanding the patient’s knowledge, history and expectations regarding prescription and non-prescription medication in the management of their dental pain.

Methods

A self-report survey was developed, using Likert-type scales, consisting of 32 items, including standardized, psychometrically-tested instruments. The survey was administered to 108 adult outpatients who reported to the West Virginia University …

Factors Related To Collaborative Willingness In Surrogate Decision-Making Situations From The West Virginia Post., Rachael Spalding

Graduate Theses, Dissertations, and Problem Reports (ETD)

If a patient becomes unable to adequately express preferences for medical care, another individual called a “surrogate” may be consulted to make treatment decisions. Surrogates may be asked to indicate patient preferences on the Physician Order for Scope of Treatment (POST), a new type of advance directive completed with patients who are estimated to have less than one year left to live. Research from the shared decision-making literature indicates that collaboration can be a valuable means of enhancing patient satisfaction with their decisions. The current study examined how certain psychological and demographic variables predicted the extent to which participants desired …

Shame And Guilt: The Good, The Bad, And The Ugly, Raika Sadeghein

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the past few decades, we have witnessed a growing body of literature on emotions in the consumer behavior domain. Emotions are defined as “mental states of readiness that arise from appraisals of events or one’s own thoughts” (Bagozzi, Gopinath, & Nyer, 1999, p. 184). Marketers have examined the role of emotions as stimuli, mediators, moderators, and responses to the environment. The broad topic of emotions includes subtopics such as impact of emotions on consumption, attitudes, and behavioral intentions, emotional responses to market stimuli, and the impact of emotions on cognition. However, although the study of emotions is pivotal to …

Compound-Specific Isotope Analysis Of Amino Acids In Biological Tissues: Applications In Forensic Entomology, Food Authentication And Soft-Biometrics In Humans, Mayara Patricia Viana De Matos

Graduate Theses, Dissertations, and Problem Reports (ETD)

In this work we demonstrate the power of compound-specific isotope analysis (CSIA) to analyze proteinaceous biological materials in three distinct forensic applications, including: 1) linking necrophagous blow flies in different life stages to their primary carrion diet; 2) identifying the harvesting area of oysters for food authentication purposes; and 3) the ability to predict biometric traits about humans from their hair.

In the first application, we measured the amino-acid-level fractionation that occurs at each major life stage of Calliphora vicina (Robineau-Desvoidy) (Diptera: Calliphoridae) blow flies. Adult blow flies oviposited on raw pork muscle, beef muscle, or chicken liver. Larvae, pupae …

The Food Bank Fix: Hunger, Capitalism And Humanitarian Reason, Joshua David Lohnes

Graduate Theses, Dissertations, and Problem Reports (ETD)

Drawing on a five-year institutional ethnography of Humanitarian Food Networks (HFNs) in West Virginia, this dissertation explores the moral, political and economic place of the food bank in the corporate environmental food regime. I develop the concept of the food bank fix to theorize the paradoxical relationships between the state, the shadow state, food corporations, local charities and food banks that tie HFNs across the United States together through humanitarian reason. I argue that food banks damp the grinding contradictions of a society awash in food surpluses even as a significant proportion of the population remains at risk of hunger. …

Appalachian Caregivers Of Persons With Dementia Non Use Of Services, Kristina M. Childers

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

Background: The purpose of this current study was to explain factors most strongly associated with Caregiver (CG) of Person with Dementia (PWD) non-use of formal and informal services in Appalachia.

Methods: A correlational explanatory design using validated self-report surveys was used to explain the association of the substructed Andersen’s Behavioral Model for Health Services Use in Appalachian CG non-use of formal and informal services. Data were analyzed using descriptive statistics and ANOVA tests considering eight independent variables in total from each research question, using a sample of 43 CG/CR dyads.

Results: The analyses of four research questions …

Characterization Of Lipids And The Protein Co-Products From Various Food Sources Using A One-Step Organic Solvent Extraction Process, Alleda Rose

Graduate Theses, Dissertations, and Problem Reports (ETD)

Traditional lipid extraction methods are labor intensive and time-consuming so interest in one-step methods using various organic solvents are gaining in popularity. Extraction efficiency depends heavily on the solvents used yet comparisons between different solvents are lacking. Defatting techniques are commonly used to concentrate crude protein and may be useful for enhancing protein content in food sources. The objective of this research was to 1) determine the lipid extraction efficiencies of hexane (H), 3:2 hexane-isopropanol (HI), chloroform (C), 2:1 chloroform-methanol (CM), and methyl-tert-butyl-ether (MTBE) using a one-step organic solvent lipid extraction method on whole insect, egg yolk, and krill powders …

Cash For Consideration? An Examination Of Academics, Demographics, And Student Socioeconomics In The Provisioning Of Institutional Aid., Matthew G. Matyasovsky

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation is an empirical investigation of student level observations of 11 cohorts of first-time full-time freshmen from a public, land-grant, research university in Appalachia. Complete with student financial aid offerings, socioeconomic status, and pre-college academic characteristics such as standardized test scores and high school GPA, I investigate the data to examine institutional behavior constrained by resource dependence. The variable of interest is institutional financial aid. Institutional financial aid awards are monetary concessions presented to students as a means to discount the overall price a student pays to enroll. As institutional aid is a direct mechanism through which the institution …

Systematic Control Of Aged Skeletal Muscle Following High-Intensity Stretch-Shortening Contraction Exercise Training: Epigenomic Regulation And Signaling Factors Underpinning Adaptation, Marshall Alan Naimo Jr.

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sarcopenia, the age-related decline in skeletal muscle mass, results in a loss of strength and functional capacity, which subsequently increases the risk of disease, disability, frailty, and all-cause mortality. Exercise is known to be an efficacious paradigm for improving health and attenuating or preventing many chronic diseases. For the previous two decades, our laboratory has established an in vivo rodent dynamometer model to explore the effects of various skeletal muscle training paradigms following stretch-shortening contractions (SSCs).The responses tohigh-intensity resistance-type exercise training (RTET) using this physiological model ranges from adaptation, characterized by enhanced skeletal muscle performance along with increased muscle mass, …

Quantifying Human Biological Age: A Machine Learning Approach, Syed Ashiqur Rahman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Quantifying human biological age is an important and difficult challenge. Different biomarkers and numerous approaches have been studied for biological age prediction, each with its advantages and limitations. In this work, we first introduce a new anthropometric measure (called Surface-based Body Shape Index, SBSI) that accounts for both body shape and body size, and evaluate its performance as a predictor of all-cause mortality. We analyzed data from the National Health and Human Nutrition Examination Survey (NHANES). Based on the analysis, we introduce a new body shape index constructed from four important anthropometric determinants of body shape and body size: body …

Kidney Ailment Prediction Under Data Imbalance, Ranaa Mahveen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Chronic Kidney Disease (CKD) is the leading cause for kidney failure. It is a global health problem affecting approximately 10% of the world population and about 15% of US adults. Chronic Kidney Diseases do not generally show any disease specific symptoms in early stages thus it is hard to detect and prevent such diseases. Early detection and classification are the key factors in managing Chronic Kidney Diseases.

In this thesis, we propose a new machine learning technique for Kidney Ailment Prediction. We focus on two key issues in machine learning, especially in its application to disease prediction. One is related …

Improving Broiler Performance Utilizing Modern Feed Additives, Niles Rourke Ridgeway

Graduate Theses, Dissertations, and Problem Reports (ETD)

Modern broiler production strives to make modest improvements regarding broiler performance. This remains a goal as global population increases thusly increasing the quantity of an affordable, high quality source of protein. In recent years, an added stressor to achieving prior goals is mostly related to consciousness of environmental and consumer health. For decades, integrators have utilized minimal amounts of antibiotics as a barrier to most pathogens affecting the microbiome of a broiler’s gastrointestinal tract. Regulatory efforts have now prohibited most of previous sub-therapeutic medicine and started an in-depth scope of broiler gut health and interaction with antibiotic alternatives. In an …

Training Friends And Overseas Relief: The Friends Ambulance Unit And The Friends Relief Service, 1939 To 1948, Nerissa Kalee Aksamit

Graduate Theses, Dissertations, and Problem Reports (ETD)

This transnational case study investigates the establishment and development of training programs by two British faith-based voluntary relief organizations, the Friends Ambulance Unit (FAU) and the Friends Relief Service (FRS), during the Second World War and explores the implementation of learned skills by members of those organizations working during the immediate postwar period in the British Occupation Zone in Germany. It contributes new perspectives to scholarship on humanitarianism as it highlights both the continuities and ruptures in the approaches to and practices of humanitarian aid. It identifies the Quaker traditions that shaped the work of the FAU and FRS—particularly the …

The Effects Hot Water Kill Time Has On Dna Degradation And Str Profiling From Lucilia Sericata (Diptera: Calliphoridae) Crop Contents, Nicholas Alexander Haas

Graduate Theses, Dissertations, and Problem Reports (ETD)

he use of entomological specimens as a source of forensic DNA evidence has been studied since the late 20th century. Currently as it stands, to preserve both the minimum postmortem interval (mPMI) and DNA evidence within fly larvae, two standards of preservation exist. For mPMI, the use of hot water kill with near-boiling water is used to fix larvae size for accurate age estimation. For DNA evidence, the standard technique of preservation is submersion in 70% ethanol. To help reduce the number of standard preservation techniques for practitioners, this study assessed the feasibility of the hot water kill preservation technique …

The Acute Effect Of Propanil On Reproductive Hormones, Ovarian Gene Expression And Splenic Cells In C57bl/6 Female Mice, Malia Danielle Berg

Graduate Theses, Dissertations, and Problem Reports (ETD)

Earlier evidence indicates exposure to propanil, a post-emergent herbicide used on rice and wheat fields, increases the number of antibody-secreting cells (ASC) in the spleen of mice after immunization with heat-killed Streptococcus pneumoniae (HKSP, 2x10⁸ CFU). Interestingly, an intact hypothalamic-pituitary-gonadal (HPG) axis is required for this immune response assessed on day 7-10 after immunization in female mice. Hence, an overarching premise is that propanil arbitrates the connection between the reproductive and immune system by altering the concentration of hormones involved in the HPG axis, which may then affect the populations of splenic cells. Hypotheses were tested with C57Bl/6 female …

Low Sucrose, Omega-3 Enriched Diet Has Region-Specific Effects On Neuroinflammation And Synaptic Function Markers In A Mouse Model Of Doxorubicin-Based Chemotherapy, Tonya S. Orchard, Monica M. Gaudier-Diaz, Panchita Phuwamongkolwiwat-Chu, Rebecca Andridge, Maryam B. Lustberg, Joshua Bomser, Rachel M. Cole, Martha A. Belury, A. Courtney Devries

Clinical and Translational Science Institute

Chemotherapeutic agents such as doxorubicin may negatively affect long-term brain functioning in cancer survivors; neuroinflammation may play a causal role. Dietary approaches that reduce inflammation, such as lowering sucrose and increasing eicosapentaenoic acid plus docosahexaenoic acid (EPA + DHA), may attenuate chemotherapy-induced neuroinflammation and synaptic damage, thereby improving quality of life. Ovariectomized, C57BL/6 mice were assigned to a chemotherapy (9 mg/kg doxorubicin + 90 mg/kg cyclophosphamide) or vehicle two-injection regimen, with injections two and four weeks after starting diets. In Study 1, mice received low sucrose diets with EPA + DHA or No EPA + DHA for four to six …

Future Implications Of Using Registered Dietitians In Multidisciplinary Polycystic Ovary Syndrome Treatment, Wendy M. Wolf, Rachel A. Wattick, Pamela J. Murray, Melanie Clemmer, Melissa D. Olfert

Clinical and Translational Science Institute

Polycystic ovary syndrome (PCOS) is the most common reproductive endocrine disorder in females with insulin resistance playing a key role in pathogenesis. The objective of this study was to investigate current trends and future implications of multidisciplinary PCOS clinics with inclusion of dietitians. A two-phase, formative investigation on practitioners was conducted through an anonymous survey followed by focus groups. Survey respondents included 261 health care providers from around the world; the majority (59%) representing multidisciplinary teams. Focus group participants included four dietitians, three physicians, a health psychologist and a licensed nutritionist. Primary barriers for future multidisciplinary clinics included: money/resources, insurance …