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Designing Dracula: A Feminist Revenge Fantasy, Really: A New Take On The New Woman, Spencer T. Lawlor

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis will document the process of designing the costumes for Dracula: A Feminist Revenge Fantasy, Really, by Kate Hamill. It will detail a close reading of the text and how it differs from its original source material, Bram Stoker’s Dracula, an examination of the research process for this design, as well as an analysis of the design process and execution of costumes for this production. It will explore the challenges I faced as the Costume Designer on this production, including but not limited to: examining the gender dynamics involved in genderbending a well-known piece of literature, working within a …

Computational Methods For Identification Of Molecular Signatures, Weijun Yi

Graduate Theses, Dissertations, and Problem Reports (ETD)

This work develops computational methods for identifying molecular signatures from high-throughput genomic data and for modeling long non-coding RNA (lncRNA) sub-cellular localization. The response of multiple myeloma to CB-6644, a selective RUVBL1/2 complex inhibitor with potential anti-tumor activity, is analyzed to identify drug-responsive pathways and molecular signatures. Conventional gene set enrichment analysis (GSEA) often excludes low-expression genes. Here, phenotype comparison is reformulated as a supervised machine learning problem: genes most informative for discrimination are first selected using a machine learning approach, and GSEA is then applied to these machine-learning derived gene sets. This framework improves detection of CB-6644-associated pathways. For …

Reducing Inorganic Phosphorus In Poultry Diets: Nutritional Constraints And Strategies For Successful Implementation, Elizabeth Ann Lynch

Graduate Theses, Dissertations, and Problem Reports (ETD)

Applied poultry nutrition and feed manufacture science can be described as a “three-legged stool” supported by pellet quality, feed hygienics, and nutrient availability, where imbalance in any one component can compromise overall bird performance. The research presented herein primarily addresses feed hygienics and nutrient availability, with pellet quality serving as an underlying theme, to better understand how processing conditions and dietary formulation influence performance and nutrient utilization.

In Chapter 2, the objective of this study was to evaluate the effects of hygienizer retention time on bacterial reduction during feed manufacture using Enterococcus faecium as a Salmonella surrogate. Pellet mill motor …

A Fine-Scale Genomic Study Of Cheat Mountain Salamanders (Plethodon Nettingi) On Managed Public Land, Amylynn Nicole Ephraim

Graduate Theses, Dissertations, and Problem Reports (ETD)

Habitat fragmentation has the potential to isolate localized populations, particularly those which are understood to have limited dispersal capabilities. This isolation can reduce genetic diversity through the effects of inbreeding depression, and small populations are more susceptible to the impacts of genetic drift. In this study, I aim to understand the level of genetic connectivity between known occupied patches of Cheat Mountain salamanders (Plethodon nettingi; CMS) on managed public land. CMS are a federally threatened, high-elevation, West Virginia endemic species associated with red spruce (Picea rubens) forests. I used double-digest restriction site associated DNA (ddRAD) sequencing …

Plant Invasion Patterns In Understory And Soil Microbial Communities Of North Central West Virginia Mixed Hardwood Forests, Isabelle Marie Uganski

Graduate Theses, Dissertations, and Problem Reports (ETD)

Invasive plants alter forest ecosystems by reducing species richness, habitat heterogeneity, and nutrient cycling as they rapidly displace native plants. Invasive plant composition can influence soil health through altering soil microbial associations and communities. By studying the mechanisms in which invasives alter above- and below-ground communities, we can better understand whole-ecosystem impacts and inform management decisions. This study examined the relationships between co-occurring invasion on above and belowground communities of mixed hardwood forests of north central West Virginia. Despite distinctive associations with aboveground characteristics (native diversity, native tree regeneration, and leaf litter density) we detected limited changes to soil microbial …

Compelled Contraception, Lars Noah

West Virginia Law Review

Can the federal government insist that people use contraceptives? It may come as a surprise to learn that the U.S. Food and Drug Administration (FDA) has done just that as a condition for allowing access to some pharmaceutical products that create a serious risk of birth defects. Over the last few decades, the agency has demanded that patients using certain teratogenic agents—now numbering over a dozen drugs prescribed for a variety of conditions, ranging from severe acne and psoriasis to multiple myeloma and pulmonary hypertension—agree to avoid becoming pregnant, sometimes for years after completing their course of treatment. Undoubtedly the …

Artificial Intelligence Introduction Section, Seth Newell, Sally Brown

Artificial Intelligence Exhibit

The introduction gives an overview of the exhibition, along with an explanation of AI literacy, AI vs. Google, and a basic AI timeline.

Rocks, Tyler Cummings

Calliope

No abstract provided.

Seoul Diptych, Abby Macneil

Calliope

No abstract provided.

Full Issue

Calliope

No abstract provided.

The Man, The Myth, The Mothman: Cryptid Folklore And West Virginian Identity Formation, Bailey South

Munn Scholars Awards

“Cryptids” as a theme within Appalachian (more specifically West Virginian) folklore has gained increased interest in recent years, yet limited scholarly attention has been paid as to why. Along similar lines, many West Virginians have reclaimed the West Virginia regional identity. In this study, I collected and analyzed the opinions of West Virginians on the West Virginia identity and on cryptids to argue that the popularity of cryptids within folklore (a reclamation) has coincided with the reclamation of the West Virginian identity. I surveyed a demographic of mainly students through an online survey to analyze their perspectives, gathering demographic data …

Generative Artificial Intelligence In Hollywood: The Turbulent Future That Lies Ahead, Zachary T. Young

West Virginia Law Review

Since the dawn of time, the human race has used some form of technology to assist their unwavering dedication to push society forward. From the first stone tool to the first computer, some sort of regulation controlled their usage, ranging from government regulations to industry standards. When artificial intelligence (“AI”) entered the arena of technology, regulatory bodies froze at the daunting task of controlling such a powerful tool. Across almost every industry, artificial intelligence has found its home in various work functions. Generative artificial intelligence has furthered the complexity that stems from regulating a new, and never before seen technology. …

“Everybody Else Seems To Be Able To Get Help”: Rural Regulation, Access Loss, And Antigovernment Sentiment, Jennifer Sherman

West Virginia Law Review

This Essay draws upon two decades of the author’s research on rural United States communities to explicate ways in which the regulatory state has intervened in rural areas and economies, often with the claim of protecting land, natural resources, and/or wildlife. The outcomes are frequently disastrous for the human populations of those communities and generally are not planned for or mitigated by policy. The Essay argues that rural distrust of and aversion to government oversight is not caused primarily by ideological or cultural stances, but rather is an outcome of experiences of abandonment by the agencies that are meant to …

Creative Energies: Unearthing The Role Of Romantic Ecocriticism, Nicole Marie Chambers-Stahl

Graduate Theses, Dissertations, and Problem Reports (ETD)

The literary field of ecocriticism has transcended the boundaries of Academia: excited to connect to their physical environments, many of these scholars are focusing on genres like place-based writing, rhetoric of energy policy, and representations of the Earth in film and media. Interdisciplinary by nature, ecocriticism has demonstrated clear potential in its ability to intersect with concentrations like the history of science, animal studies, and environmental justice.

However, as the language around global crises is heating up —“Today it is scientists, not prophets,” writes Tobias Menely in “‘The Present Obfuscation,’” “who speak the language of apocalypse” (478)—ecocritical scholarship is at …

The People Of The North: Influencer Histories And Miniaturization In Eleventh-Century Italy, Evan Anthony Simonetti

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Norman invasion of Italy in the eleventh century was a process that required political support and legitimacy for its leaders, the Hauteville dynasty. The members of this dynasty either commissioned or financed three individuals as they wrote three respective histories of the Norman conquest in order to accomplish this legitimation. In these sources, each author took inspiration from the classic works of history and poetry of the Greek and Roman world and applied symbolism and direction from their understanding of medieval Christian theology. In addition, these works catalogued examples of events, people, places, animals, and behavior as exotic objects …

Behavioral Effects Of Prenatal Ecig Aerosol Exposure On Sprague-Dawley Rats, Erin Elizabeth Wylie

Graduate Theses, Dissertations, and Problem Reports (ETD)

Maternal electronic cigarette (ECIG) use during pregnancy raises concerns about potential risks to offspring, yet its behavioral effects remain unclear. The present studies were designed to investigate how prenatal exposure to ECIG aerosols, with and without nicotine, influences the behavior of Sprague-Dawley rat offspring. Dams were exposed to aerosols containing base liquid alone, aerosols containing base liquid with 18 mg/ml nicotine, or filtered air (control) throughout gestation. Following postnatal day 65, male offspring from 17 litters completed lever-press acquisition (Experiment 1), sustained-attending (Experiment 2), and nicotine self-administration (Experiment 3) tasks. One-way analyses of variance (ANOVAs) were used to compare task …

Motivation And Well-Being: A Study Of Wvu Extension Volunteers, Amber Renae Goff

Graduate Theses, Dissertations, and Problem Reports (ETD)

Volunteers are the heart of the land-grant outreach model. They bring education and resources to communities across West Virginia. Volunteers have been relied upon in many aspects of Extension programming and current budget conditions have reduced the level of staffing within WVU Extension. There may be an increased demand for volunteers to help with programming. The purpose of this study was to investigate the relationship between motivation and the perceived level of well-being within the WVU Extension volunteer programs. The study yielded 464 responses from a descriptive survey. Based on the results, WVU Extension volunteers are motivated based on their …

Dynamically Scaling Biomimetic Robots Through Parallel Viscoelastic Actuators, Foster Olaf Holmquist

Graduate Theses, Dissertations, and Problem Reports (ETD)

Robots provide a unique proving ground for testing scientists’ understanding of neuromuscular systems. Synergistically, mimicking biological control laws and mechanics could offer robotics the robust and adaptive locomotion of animals. Previous work has utilized the wealth of research on insect biomechanics to develop scaled robotic models but doing so presented a problem: while these robots’ larger scale has simplified manufacturing, the scale has also shifted its mechanics from those of the modeled organisms. While animals walk, forces are generated that describe their mechanics and influence motor control. The musculoskeletal structure and stretch of muscles result in viscous and elastic forces, …

The Effect Of Cerebellar Feedback On The Spatial Processing Of Conspecific Signals In The Electrosensory System, Dakota Ray Miller

Graduate Theses, Dissertations, and Problem Reports (ETD)

Animals have evolved sensory systems to help decipher important environmental signals that are vital for their survival. Feedback pathways are necessary to allow these signals to stand out in a noisy background, enabling the animal to focus on them more effectively. The electrosensory system of Apteronotus leptorhynchus allows the fish to sense the modulations caused by other conspecifics to allow for proper responses during social interactions. Electroreceptors detect these EODs, and afferent nerves project these signals to the ELL, where electrosensory information is projected to other parts of the brain. Cerebellar feedback to the ELL allows the suppression of lower …

3d Printed Microfluidic Platforms For Elisa-Based Diagnostics: Advancing Toward Point Of Care, Brandi Marie Binkley

Graduate Theses, Dissertations, and Problem Reports (ETD)

Protein biomarker detection is a critical analytical method for quantifying proteins in biological samples. It is relevant not only in medical diagnostics and research but also numerous other fields such as environmental monitoring, pharmacology, food safety, and biotechnology. Sensitive detection at picogram-per-milliliter (pg/mL) levels is essential for diagnosing diseases, monitoring progression, and tailoring treatments, especially for low-abundance biomarkers like troponin, interleukin 6, carcinoembryonic antigen (CEA), activin A, and vascular endothelial growth factor (VEGF). Immunoassays, particularly enzyme-linked immunosorbent assays (ELISAs), are the gold standard for protein biomarker detection due to their high specificity and sensitivity. The development of point-of-care (POC) diagnostic …

A Limb-Speed-Driven Locomotor Control System And Its Ability To Adapt, Emily Marie Herrick

Graduate Theses, Dissertations, and Problem Reports (ETD)

Despite how simple walking may seem, the locomotor control system is structurally and functionally complex. Its hierarchical organization of supraspinal and spinal networks with forward and feedback pathways has many interactions at multiple levels that are dependent on the dynamics of a high-dimensional musculoskeletal system. Having a comprehensive understanding of sensorimotor integration within a healthy locomotor control system is crucial for understanding changes to the system due to age or neurologic disease and developing effective technologies to recover mobility in those populations. In this dissertation, we address persistent gaps in knowledge pertaining to how the nervous system controls locomotion.

In …

Co-Transmission Is Co-Opted For The Coordination Of Auditory Sensory Integration, Marryn M. Bennett

Graduate Theses, Dissertations, and Problem Reports (ETD)

Corollary discharge circuits (CDCs) are conserved mechanisms for sensory modulation during species specific behavioral programs. Many behaviors require multiplexed differential sensory modulation for proper execution. Co-transmission of fast and slow neurotransmitters allows for distinct modulation of different downstream targets within one sensorimotor circuit. However, studying co-transmission within the context of modulatory circuits like CDCs requires complete knowledge of behavioral circuits and precise tools for individually determining the implications of each transmitter on its targets. In this dissertation, I used a conserved set of corollary discharge interneurons in Drosophila melanogaster as a model to study the intricacies of sensory modulation through …

Investigating The Biology, Hypovirulence, And Pathogenicity Of Three Canker-Causing Phytopathogenic Fungi In The Cryphonectriaceae And Cytosporaceae On American Chestnut (Castanea Dentata) And Oaks (Quercus Spp.) In The Eastern U.S., Danielle N. Mikolajewski

Graduate Theses, Dissertations, and Problem Reports (ETD)

American chestnut (Castanea dentata) and various oak species (Quercus spp.) are impacted by several economically important canker pathogens in the fungal order Diaporthales, namely Cryphonectria parasitica and Endothia gyrosa (Cryphonectriaceae). Cytospora canker caused by several closely related fungi in the fungal genera Cytospora and Valsa (Cytosporaceae) have also been implicated in causing canker diseases of Quercus and Castanea in Asia, Europe and North America. The impacts of C. parasitica on American chestnut have been exhaustively studied and it’s impacts on various oak hosts have also been broadly investigated and reported. Endothia gyrosa has …

Rare Earth Element Recovery From Acid Mine Drainage Using Ion Exchange And Selective Precipitation Processes, Paubla Andrea Gallego Lopez

Graduate Theses, Dissertations, and Problem Reports (ETD)

Rare earth elements (REEs) are used in high-tech applications due to their electromagnetic and optical properties. As global demand for REEs surges, the limitation of primary mineral deposits has increased interest in alternative sources. One such source is acid mine drainage (AMD), which, although typically low in REE concentration, offers a promising avenue for recovery. Among the emerging recovery methods, ion exchange has shown promise due to its effectiveness at low concentrations and lower operational cost than conventional techniques such as acid leaching and solvent extraction.

This research focused on developing a recovery process by combining ion exchange with selective …

Characterization Of A Descending Neuron That Modulates Flight Performance And Provides Input Onto A Flight-Related Predictive Motor Circuit, Andisheh Balouchi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Descending neurons (DNs) play a critical role in sensorimotor integration by relaying motor information to the ventral nerve cord (VNC). Within the VNC, local interneurons, other DNs, and ascending neurons (ANs) make circuits with sensory and motor neurons to modify flies’ behavior. One important AN pair in Drosophila Melanogaster is mesothoracic ascending histaminergic neurons (MsAHNs), which are known to convey predictive wing motor signals to the brain. DNg32 is the primary upstream synaptic partner of MsAHNs, integrating DNg32 input with other inputs to generate predictive motor signals. The goals of this study were to 1) map the synaptic connectivity of …

Simulated Slot Machine Gambling In Relation To Sign-Tracking, Polina Krom

Graduate Theses, Dissertations, and Problem Reports (ETD)

The propensity to attribute incentive salience to reward-related cues, or sign-tracking (ST) has been linked to addiction in animal models and some initial human data. The current study explores the role of sign-tracking (ST), as a potential risk factor for problematic slot machine gambling, which abundantly features audiovisual reward-related cues, henceforth sensory features. A sample of healthy participants without gambling problems (n = 30) completed a Pavlovian-to-Instrumental Transfer (PIT) paradigm to assess ST propensity and played on a realistic slot machine simulator consisting of two versions: with sensory features (SF+) and without (SF-). Each game version had fixed bet sizes, …

Using Data Science And Sustainability Assessment Tools To Evaluate Wastewater Treatment Technologies, Haidar Shakuir Aldaach

Graduate Theses, Dissertations, and Problem Reports (ETD)

Wastewater management in small communities has faced numerous challenges in recent years, including population growth, limited budgets for operation and maintenance, and stringent effluent standards required by environmental regulations. These challenges motivate enhancing wastewater treatment processes to protect water quality. Wastewater treatment performance can be improved in small towns and rural regions by implementing data-driven strategies. Data science, life cycle assessment (LCA), and life cycle cost analysis (LCCA) are three data-driven decision-making tools that require data collection, modeling, computational models, and statistical analysis that could be utilized to improve wastewater treatment efficiencies. In this study, these three tools were adopted …

Tobacco And Trouble: Exploring The Health Consequences Of Electronic Cigarette Use In Adolescents, Noor Ibrahim Abdulhay

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Tobacco use remains one of the leading causes of preventable disease and death in the United States (U.S.). Although conventional cigarette use among youth has declined, the use of electronic cigarettes (e-cigarettes) has become highly prevalent and poses emerging public health concerns. Adolescence represents a critical developmental period for both oral and mental health, making it essential to understand the consequences of tobacco and nicotine exposure during this stage. The goal of this dissertation is to examine the health impacts of tobacco use in adolescents across multiple domains. Specifically, this research (1) synthesizes existing evidence on the relationship between …

The Dna Methylation Capacity Of The Tsetse Fly: Glossina Morsitans, And Its Integration With Symbiont Nutrient Provisioning, Dylan Forrest Richmond

Graduate Theses, Dissertations, and Problem Reports (ETD)

Tsetse flies (Diptera: Glossinidae) are the vector of trypanosomes (Trypanosoma spp.), which are responsible for African trypanosomiasis, also known as sleeping sickness. African trypanosomiasis is a significant health and economic burden yet remains a neglected tropical disease. As an obligate blood feeder, tsetse flies are reliant on B vitamin supplementation from intracellular Gammaproteobacteria, Wigglesworthia glossinidia. Folate (B9) is essential for tsetse development and reproduction, as well as DNA methylation in animals. As a reversible modification, DNA methylation typically occurs in a CpG dinucleotide context and can alter gene expression via the restriction of transcription. Across insects, the …

Power, Economy, And Food Systems In The Confederacy: A Material History Of The Confederate Subsistence Department, John Martin Mcmillan

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation questions how human and non-human actors share power. By exploring Confederate logistics within the Subsistence Department, the collaboration between bureaucrats and the material world is revealed. Contributing to scholarship aimed at expanding the field of Civil War studies, this dissertation not only provides a more complex and interesting view of the conflict, but also an understanding that human beings are not solely responsible for the war’s results. By applying a materialist approach, the causes and outcomes of an event as complicated as a civil war can be better explained and understood. The choice to stockpile food for distribution …