Digital Commons Network^™

Ciliary Arl14b In Vision, Alexis Breann Crockett

Graduate Theses, Dissertations, and Problem Reports (ETD)

Primary cilia are highly specialized organelles that regulate signaling, trafficking, and sensory perception across multiple tissues, including the retina. The small GTPase ADP Ribosylation Factor-Like Protein 13B (ARL13B) is an atypical ARF-family protein enriched in cilia. ARL13B acts as a guanine exchange factor (GEF) for another small GTPase ARF-like Protein 3 (ARL3). Mutations in ARL13B cause Joubert syndrome, a ciliopathy characterized by brain malformation, developmental delays, and retinal degeneration. Despite ARL13B’s known ciliary localization and clinical relevance, ARL13B’s precise functions in cilia remain poorly defined. This dissertation investigates the cilia-specific roles and GEF-specific roles of ARL13B in photoreceptors using two …

Evaluation Of Small Unmanned Tailsitter Hybrid Air Vehicles Via Full Flight Simulation, Andrew Spencer Winters

Graduate Theses, Dissertations, and Problem Reports (ETD)

Small Unmanned Aerial Vehicles have exploded in popularity in the past 15 years among researchers and for industrial uses such as agriculture, search and rescue, and infrastructure inspection. Fixed wing and multirotor designs present two major paths that can be taken with these technologies. Fixed wing platforms have superior aerodynamic efficiency but require large areas for takeoff/landing and cannot hold a constant position in space, limiting their use. Multirotor configurations offer a more versatile platform that can maneuver in 3 dimensions independently but lack the endurance of fixed wing vehicles. This thesis analyses a quadrotor biplane and a variable wingspan …

Raman Spectroscopy For Rapid Quantification Of Macronutrients In Heirloom Beans, K. Chandler Williams

Graduate Theses, Dissertations, and Problem Reports (ETD)

Quantification of macronutrients in food requires a large investment in facilities and instrumentation as well as ongoing expenses of labor, reagents, and hazardous waste disposal. These costs pose a significant barrier to determining macronutrient quantities in the thousands of uncharacterized landrace and heirloom crops and discourage traditional crop breeding projects from ensuring that their selected crops maintain desirable macronutrient profiles in addition to target agronomic traits. Our objective was to test whether a hand-held Raman spectrometer—a technology which has found use in plant disease diagnostics but remains largely overlooked in nutrient quantification-- could provide a feasible means for quantifying the …

A Post Apocalyptic Romp: Designing Costumes For Phoebe In Winter, Emmalee Berger

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis will document the process of costume designing for Phoebe in Winter by Jen Silverman. It will examine the process from first read of the script through initial and final design meetings and to execution and production of the design. This will report on challenges specific to this production, such as nudity and violence on an academic stage, and the subsequent solutions. Phoebe in Winter was produced by West Virginia University School of Theatre and Dance in October of 2024. The design process began in February of the same year.

A Comparison Of Endodontic Root-End Filling Techniques: A Micro-Computed Tomography (Micro-Ct) Analysis Part Ii, Andrew M. Marra

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

A COMPARISON OF ENDODONTIC ROOT-END FILLING TECHNIQUES: A MICRO-COMPUTED TOMOGRAPHY ANALYSIS

Andrew Marra, D.D.S.

Introduction: Researchers have compared many endodontic root-end filling techniques for porosity, though few have used a micro-computed tomography analysis for comparisons. The purpose of this study was to evaluate total porosity volume of three different bio-ceramics filling materials with two techniques--hand compaction versus hand/ultrasonic compaction at an ultrasonic setting of 5% using Micro-CT Analysis.

Methods: Seventy-two human single canaled extracted teeth were selected for the study. All were subjected to orthograde endodontic treatment followed by root tip resection at 3-mm from the apex …

Thermal Inactivation Of E. Faecium On Ground Poultry, Joseph Katz

Graduate Theses, Dissertations, and Problem Reports (ETD)

Chicken products in grocery stores are contaminated with Salmonella that is ranked number one as a foodborne pathogen, and causes about 1.35 million cases of foodborne illness, 26,500 hospitalizations, and 420 deaths annually. The objective of this study is to determine thermal kinetic parameters of Salmonella surrogate bacteria E. faecium in ground poultry as affected by different temperatures and salt concentrations. The raw chicken was sliced up into a 500 g batch with a total of 6 batches per replicate, ground up in the meat grinder followed by inoculation with E. faecium resistant to 200 ppm Nalidixic Acid. NaCl …

An Edna Assessment Of The Diamond Darter (Crystallaria Cincotta) In Its Current And Historic Distribution Range, Cameron Moore Layne

Graduate Theses, Dissertations, and Problem Reports (ETD)

Environmental DNA (eDNA) can serve as a useful monitoring tool for cryptic and imperiled freshwater stream fishes. eDNA can fill knowledge gaps regarding species’ distribution, site abundance, and habitat usage. The last known stronghold of the Diamond Darter (Crystallaria cincotta) in the Elk River of West Virginia provides an excellent test case to examine the utility of this non-invasive method of surveying rare species. Diamond Darters are one of the most newly recognized freshwater fishes in North America and are among the most imperiled U.S. fishes, having been federally listed as endangered in 2013. The cryptic behavior and limitations of …

The Effect Of Sars-Cov-2 On Bacterial Diversity And Abundance In Community Wastewater, Joshua Lambert

Graduate Theses, Dissertations, and Problem Reports (ETD)

Severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) enters human respiratory cells through binding of its spike (S) protein to the ACE-2 receptor, which is highly expressed in lung cells, but is also found in over 150 other cell types encompassing major organs including GI enterocytes. SARS-CoV-2 causes gut dysbiosis in infected individuals and patients with COVID-19 and also coincides with lower alpha diversity of the individual’s gut microbiome. Certain gut microbiome-associated genera are lower in abundance in COVID-19 patients including Lachnospira, Prevetolla, Roseburia, Bifidobacterium, and Faecalibacterium while genera such as Bacteroides, Enterococcus and Proteobacteria are increased during a SARS-CoV-2 …

Sleep, Endocannabinoid Signaling, And Cortical Processing In Mouse Models Of Autism Spectrum Disorder, Bryan Rodriguez

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sleep plays a crucial role in memory consolidation, neuronal plasticity, and cognitive function. Individuals with autism spectrum disorder (ASD) often experience sleep disturbances, which are positively correlated with symptom severity. However, on the surface, sleep architecture is remarkably normal under baseline conditions in many rodent models of ASD. The goals if this dissertation were to investigate whether regulation of sleep, biochemical signaling during sleep, and downstream consequences of sleep on behavior are altered in two mouse models of ASD. In Aim 1, we tested the hypothesis that sleep phenotypes emerge in ASD models under conditions of high sleep pressure. We …

Scenic Design For Jen Silverman’S Phoebe In Winter, Jess A. Ford

Graduate Theses, Dissertations, and Problem Reports (ETD)

This document is a narrative of the Scenic Design process for Jen Silverman’s Phoebe in Winter, designed by Jess Ford. The process includes a detailed summary and analysis of the script, the stages in the creation of the design, the final design, build, technical rehearsals, and the final product. This production was produced by West Virginia University’s School of Theatre & Dance during the Fall 2024 semester, presented in the Gladys G. Davis Theater at the Canady Creative Arts Center in Morgantown, WV.

Molecular Insights Into The Impact Of Bone Marrow Stroma, Cb-6644 Inhibition Of The Ruvbl1/2 Complex, And Cebpβ On Regulating Drug Sensitivity In Multiple Myeloma, Sebastian Adam Dziadowicz

Graduate Theses, Dissertations, and Problem Reports (ETD)

Multiple myeloma (MM), is a hematological cancer originating in plasma cells. Currently multiple myeloma represents a significant clinical challenge due its incurable nature mainly due to inevitable drug resistance. MM is characterized by abnormal plasma cell proliferation within the bone marrow and production of abnormal immunoglobulin resulting in a plethora of physiological abnormalities. Although a rare malignancy, MM accounts for 1% of all cancers and currently has five-year survival rate of around 50%. Utilizing a combination of laboratory experiments and bioinformatics techniques, this dissertation examines multiple layers of MM physiology in the context of regulating drug resistance or sensitivity. First, …

Effects Of Pubertal Stress On Later Pup-Directed Behavior In Pregnant And Non-Pregnant Adult Female Mice, Marissa Marylin Nicodemus

Graduate Theses, Dissertations, and Problem Reports (ETD)

Beginning in adolescence and throughout adulthood, women are at an increased risk for anxiety and mood disorders compared to men. Several risk factors for these disorders have been identified, but it is not clear how they interact. One such factor is pregnancy. The normative changes to the brain and behavior that occur during and after pregnancy are associated with an increased risk for anxiety and mood disorders. Exposure to adverse childhood experiences (ACEs) has also been associated with an increased risk of mood disorders, and compared to men, women appear to be particularly vulnerable to the lasting effects of stress …

Exploration Of Occupational Therapy's Consultative Role For Social Emotional Development In The Rural Childcare Setting, Katherine M. Conley

Graduate Theses, Dissertations, and Problem Reports (ETD)

Little research has been conducted investigating the consultative role of occupational therapy (OT) in childcare, especially their role in supporting the social and emotional development of children aged 1 – 5 years. This project takes place in a rural childcare setting where a needs assessment found gaps in classroom routines' functionality, the accessibility of center-wide age-appropriate resources, and the use of social and emotional supports in the classroom. The occupational therapy doctoral student utilized the coaching model, evidence-based research, and the foundations of occupational therapy with the intention to improve teachers’ confidence in implementing social and emotional content in the …

Abundance Estimates And Habitat Use Of The Candy Darter In The Critical Habitat Designation Of The Gauley River National Recreation Area, David Cole Hoffman

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis includes (1) a literature review chapter and (2) a research chapter that examines the abundance and habitat use of Candy Darters (Etheostoma osburni) in the Gauley River National Recreation Area (GARI). The Candy Darter, a small-bodied fish in the family Percidae, is an endemic and a federally endangered species in the New River drainage of West Virginia and Virginia. One of the critical habitat designations for this species is located within GARI, a reach of the river impacted by didymo (Didymosphenia geminata), where snorkeling and underwater observations were conducted to assess species abundance and …

The Professional Backgrounds And Experiences Of Higher Education Trustees And Its Influence On Decision Making, Jade J. Irving

Graduate Theses, Dissertations, and Problem Reports (ETD)

There are many complex decisions that must be made among the board of governors within institutions of higher education. Recent headlines on the decisions of trustees regarding campus closures and consolidations, program cuts, and presidential appointments have put a spotlight on the individuals responsible for making such decisions. This study examined how trustees of public 4-year institutions in the state of West Virginia perceive the value of their professional backgrounds and experiences in fulfilling their governance responsibilities. Using an explanatory sequential design, this study investigated how trustees’ professional experiences shape their decision-making processes through the theoretical lenses of sensemaking theory …

Supporting The Social Emotional Needs Of Preschoolers, Giorgi Morrone

Graduate Theses, Dissertations, and Problem Reports (ETD)

Social-emotional competence is a prerequisite to school performance and participation in daily tasks. Children in West Virginia face social and emotional barriers including the effects of the opioid epidemic leading to children placed in foster care, born with neonatal abstinence syndrome, and being exposed to toxic stress in the home environment. Additionally, the COVID-19 pandemic has impacted children's social-emotional functioning due to isolation periods. Social-emotional programming can improve children's well-being, school performance, relationships, and outcomes later in life. Occupational therapy practitioners can use data-driven decision-making and relationship-centered practice to develop group-based, health-promotion social-emotional programming in the preschool setting. The purpose …

Romantic Materialities And The Politics Of Care: Negotiating Movement In A More-Than-Human World, April J. Mcginnis

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation explores the ecological politics of movement in Romantic literature. It contributes to a growing body of scholarship that investigates the important role that walking played in nineteenth-century literary culture. Many authors from this period cast walking as a practice that frees up not only the body but the mind as well, as the steady rhythm of the feet lulls the walker into a state of self-absorbed introspection and heightened creativity. Because of this, walking was a celebrated part of the creative process for many writers. The freedom they celebrate is possible, however, only in spaces that are level, …

Inactivation Kinetics Of Escherichia Coli And Listeria Monocytogenes In Edible Insects, Precious Aduloju

Graduate Theses, Dissertations, and Problem Reports (ETD)

Edible insects are emerging as sustainable and nutritious alternatives to conventional protein sources. However, concerns about food safety, particularly the presence of pathogenic bacteria such as Escherichia coli and Listeria monocytogenes, remain a barrier to consumer acceptance and industrial use. This study evaluated the effectiveness of thermal processing in inactivating E. coli, L. monocytogenes, and their non-pathogenic surrogates (E. coli JM109 and Listeria innocua) in both fresh and dehydrated forms of Tenebrio molitor (mealworm) and Acheta domesticus (cricket). It also evaluated the influence of insect species, moisture content, and temperature on microbial inactivation. A microbial challenge …

Mourning And Haunting In Irish Gothic Fiction From The Victorian Era To Modernism, Wenyu Kong

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation explores how three Irish modernist writers, Elizabeth Bowen, Dorothy Macardle, and James Joyce, incorporate and reimagine tropes from the Victorian Gothic Revival to address Ireland’s uneven modernity in the first half of the twentieth century. I argue that the Irish Gothic tradition is essential for these writers to express their reflection and reaction to ongoing cultural and social turmoil in Ireland’s process of modernization during the foundation of the postcolonial Irish state. Extending Sheridan Le Fanu’s Big House Gothic legacy, Elizabeth Bowen creates haunted spaces in The Last September (1929) to describe Anglo-Ireland’s precarious situation during the War …

Rifles And Riflemen: Material Culture, Violence, And Early American Identity, 1720 -- 1820, John Bradstreet Weaver

Graduate Theses, Dissertations, and Problem Reports (ETD)

The rifle was one of many material objects which colonists of British North America brought with them from Europe. Starting in the early eighteenth century, German-speaking settlers in Pennsylvania began to manufacture rifles from locally made and imported parts. These rifles became associated with other material objects – namely a system of dress, which included wearing a whitetail deer’s tail in the hat, the hunting shirt, and forms of Native American dress – which became emblematic of some British colonists who used rifles in hunting and war. Rifles became militarily important in the Seven Years’ War, where Native American warriors, …

Three Essays On Sustainability Economics, Hélène Flore Nguemgaing

Graduate Theses, Dissertations, and Problem Reports (ETD)

As global economies face increasing resource dependencies and supply chain challenges, it becomes essential to understand the vulnerabilities that threaten economic stability. This dissertation investigates three empirical issues in agribusiness and critical minerals using econometric models to estimate the following: 1) the impact of supply chain disruptions on stock market returns during COVID-19; 2) the extraction of rare earth elements from acid mine drainage treatment by investigating the impacts of ownership and legislation in West Virginia and Pennsylvania; 3) China-to�FOBb (Free-on-Board) price transmission in rare earth elements markets: a copula-based dynamic tail dependence analysis.

The first essay evaluates how events …

Machine Learning And Uass For Managing Autumn Olive (Elaeagnus Umbellata) On Reclaimed Surface Mines, Sean Michael Keane

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research introduces an innovative approach for managing autumn olive (Elaeagnus umbellata) on reclaimed surface mines through the integration of machine learning algorithms and unmanned aerial systems. The proliferation of autumn olive presents significant ecological challenges on disturbed landscapes, necessitating more efficient detection and control methods. This study evaluated Random Forest classification and deep learning approaches using the U-Net architecture for identifying autumn olive across four phenological stages using high-resolution imagery. The RGB U-Net model achieved F1-scores of 0.927, 0.912, and 0.871 for autumn olive detection during peak, late, and senescence stages respectively, outperforming both multispectral and vegetation …

Comparison Of Compositional Characteristics Of Protein Extracted From Spent Hemp Seed Cakes By One-Step Defatting With Organic Solvents Followed By Isoelectric Solubilization Precipitation Using Strong And Weak Acids, Miah Nicole Gathman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Hemp seed cakes (HSC) are the byproduct of pressing hemp seeds for oil and are a significant agricultural waste product, with hundreds of tons produced annually. These nutrient-rich cakes have potential for sustainable applications in animal feed and food products. However, they could offer even greater value to the food industry through further extraction of the lipids and separation of the proteins they contain. Protein is a key nutritional component and companies are seeking cost-effective, time-efficient processes to concentrate protein from underutilized resources like HSC. Hexane (HEX) is a common solvent used in one-step defatting processes but may cause issue …

Evaluating The Adaptation Of Taro (Colocasia Esculenta) To West Virginia Climatic Conditions, Kayode Joseph Ajayi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Climate change impacts agriculture and food security across the globe, therefore higher temperatures could increase in the length of the growing period and new crops would take advantages of. Research was conducted in Morgantown, WV in 2022 and 2023 to understand the growth pattern of Taro in West Virginia, and to analyzing the chemical composition of the Taro plant. The experiment was a completely randomized design in direct planting-low tunnel technology (2 replications, and 10- plants/plot), and two fertilization treatments (fertilized and non-fertilized). Spacing was 1 m within and between rows. Data were subjected to ANOVA using SAS. Dependent variables …

The Characterization Of Il-27 Production And Its Impact On T Cell Antigen-Specificity And Diversity In A Neonatal Bcg Vaccination Model, Ashley M. Divens

Graduate Theses, Dissertations, and Problem Reports (ETD)

Tuberculosis (TB) is caused by Mycobacterium tuberculosis (Mtb), and it has plagued humans for most of recorded history. Despite strong efforts to prevent and treat TB, it remains the leading cause of death due to a pathogen and is most prevalent in certain regions of Asia and Africa. Currently, the only approved vaccine to prevent TB is the live-attenuated Bacillus Calmette-Guérin (BCG) vaccine. Most countries have a universal BCG vaccination policy, but the vaccine ultimately fails to be protective. The BCG vaccine is most effective in children and protects against disseminated TB, but protection wanes 10-15 years after vaccination causing …

Five-Year Analysis Of Kiko Buck Growth Rate, Carcass Muscling And Parasite Resistance In A Feedlot Setting, Garrett Thomas Vaughan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Five-year analysis of Kiko buck growth rate, carcass muscling and parasite resistance in a feedlot environment

Garrett T. Vaughan

From 2018 to 2023, Kiko bucks born from January 1^st to March 15^th were performance tested at West Virginia University to evaluate growth rate, ultrasound carcass characteristics and parasite resistance under an artificial infection. Kiko bucks were submitted by producers from 17 different states and over 5 performance tests, 830 Kiko bucks were evaluated. Bucks were group housed and fed ad libitum with a 16% CP pellet containing soyhulls, serving as the dietary fiber component. Upon arrival, bucks were …

A Vegan Diet Compared To A Mediterranean Diet On Plasma/Serum Cholesterol Concentrations In Hypercholesterolemic Adults, Karina Bharat Patel

Graduate Theses, Dissertations, and Problem Reports (ETD)

Hypercholesterolemia, or atherosclerotic cholesterol, is characterized as elevated concentrations of total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C), and non-high-density lipoprotein-cholesterol (non-HDL-C). According to the Academy of Nutrition and Dietetics (AND), vegan diets have proven to be the most effective diets for improvement of heart disease risk factors. On the other hand, the American Heart Association (AHA) recommends the Mediterranean-style diet as a diet pattern for the prevention of heart disease, stroke, and reduction of risk factors, such as obesity, diabetes, hypercholesterolemia, and hypertension. Therefore, the current literature review uses a systematic approach to examine the effects of a vegan diet compared …

Engineering Nanomaterials From Phase-Change Biological Materials Reacting With Carbon Dioxide, Qingyang Li

Graduate Theses, Dissertations, and Problem Reports (ETD)

The current global situation concerning CO2 emissions is increasingly alarming, with rising concentrations of CO2 significantly worsening climate change and global warming. Atmospheric CO2 levels are now at their highest in millions of years, driving a host of catastrophic effects, including but not limited to extreme weather events, rising sea levels, ocean acidification, disrupted ecosystems, deteriorating air quality, respiratory health issues, heat-related illnesses, threats to food and water security, and escalating social and geopolitical tensions. To keep global warming to no more than 1.5°C higher than that of pre-industry – as called for in the Paris Agreement – emissions need …

Improving Food Safety Practices Among Fsma-Exempt Small-Scale Produce Growers: A Manuscript-Based Dissertation On Risk, Adoption, And Practical Barriers, Jesica Marie Temple

Graduate Theses, Dissertations, and Problem Reports (ETD)

ABSTRACT

Improving Food Safety Practices Among FSMA-Exempt Small-Scale Produce Growers: A Manuscript-Based Dissertation on Risk, Adoption, and Practical Barriers

Jesica Temple

Foodborne illness outbreaks associated with fresh produce continue to pose a public health risk in the United States. Although the Food Safety Modernization Act (FSMA) Produce Safety Rule (PSR) established science-based standards to prevent contamination, many small-scale farms are exempt from these requirements. Consequently, voluntary adoption of Good Agricultural Practices (GAP) varies widely across regions. Strategies to improve engagement among FSMA-exempt growers are key to advancing postharvest food safety and reducing contamination. This manuscript-based dissertation examined how knowledge, attitudes, …

Recovery Of Iron And Aluminum From Acid Mine Drainage For Beneficial Uses, Katelyn Renee Pepe

Graduate Theses, Dissertations, and Problem Reports (ETD)

Acid mine drainage (AMD) is a widespread issue across the globe, in which some of the most critical cases are created by the mining industry. Researchers have investigated ways to derive benefits from AMD, such as precipitating rare earth elements (REEs) to generate potential revenue from AMD treatment and supplement market demand. However, the bulk of precipitated sludge is composed of iron and aluminum, which also have market value but are discarded in current practice.

Using the Omega site in West Virginia (WV) as a representative AMD source, the objectives of this study are (1) to determine a replicable precipitation …