Digital Commons Network^™

Genomic Variation, Adaptive Evolution, And Invasive And Evolutionary History Of Microstegium Vimineum, Cameron William Corbett

Graduate Theses, Dissertations, and Problem Reports (ETD)

Invasive species can cause environmental and economic damage, but can offer unique opportunities to study rapid evolution and adaptation in novel environments. Microstegium vimineum (stiltgrass) is a prolific invasive grass in the eastern United States. Historical herbaria collections were used to identify patterns of invasion history and population dynamics over a spatial and temporal scale. Analyses of plastid genomes reveal a complex invasion history, including a single introduction in the southern United States linked to Japan and multiple repeated introductions in the northern United States from various source populations. Spread of introduced populations provide evidence of admixture among previously geographically …

Treatment Of Birdsong In Selected Works For Solo Piano, Madeline Hoover

Graduate Theses, Dissertations, and Problem Reports (ETD)

Birdsong-inspired music makes up a small but important part of the classical piano repertoire. This paper reviews birdsong in classical music since the seventeenth century, exploring different techniques for mimicking birdsong and the unique strengths of the piano as an instrument for purveying bird music. In the Baroque and Classical eras, the cuckoo song was sampled often because of its simplicity. Later, composers’ attention turned toward more complicated songs, like that of the hermit thrush. Interest in more complex songs led to experimentation in notation and a push for more accurate transcriptions. In the 20th century, composers included recordings of …

Ornithine Metabolism In Retinal Pigment Epithelium And Retina And Implications In Gyrate Atrophy, Artjola Puja

Graduate Theses, Dissertations, and Problem Reports (ETD)

Ornithine aminotransferase (OAT) is a mitochondrial enzyme that regulates ornithine metabolism by converting ornithine to pyrroline-5-carboxylate, a precursor for proline and glutamate synthesis. Deficiency of OAT causes systemic ornithine accumulation and leads to gyrate atrophy (GA) of the choroid and retina, a hereditary disorder characterized by progressive chorioretinal degeneration and irreversible vision loss. Chorioretinal atrophy typically starts at the mid-peripheral retina and progresses toward the macula. Although elevated ornithine is considered toxic to the eye, how ornithine is metabolized in ocular tissues and why OAT deficiency causes selective, region-specific vulnerability remain unclear. We combined ex vivo and in vivo stable …

Process Optimization And Quality Control In Manufacturing Of Frp Composites By Vartm, Mohamed Emadeldin Omar Abdelhadi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Railroad tank cars, being one of the most used means of transportation of hazardous material across the country, have not been structurally upgraded for a long time. Thus, the Pipeline and Hazardous Materials Safety Administration (PHMSA), a United States Department of Transportation (USDOT) agency, released a rule for safer transportation of highly hazardous and flammable liquids by railroad to enhance the design specifications of both the new and existing tank cars in May 2015. A multifunctional composite jacket is proposed as a new material to structurally enhance the railroad tank cars to withstand any accidents that could happen en route. …

Do You Walk On By? The Effects Of Donor Fatigue On Willingness To Donate, Shawn Michael Henry

Graduate Theses, Dissertations, and Problem Reports (ETD)

Donation solicitation literature validates the successful usage of various solicitation tactics that lead to achieving set donation goals for their associated cause. Yet, while the financial success of “checkout charity” is well-documented, a significant gap remains regarding the impact of high-frequency solicitations on long-term consumer-brand relationship. This research addresses this gap. Over the span of three surveys, this study finds that donation request frequency overall erodes donation likelihood through donor fatigue when donation frequency changes (occasional vs. always). However, this relationship is moderated by the perceived consumer base, with a larger perceived consumer base exacerbating the “diffusion of responsibility.” Further, …

Comparison Of Push-Pull Air Curtain To Overhead Downdraft Systems On Aerosol Containment And Distribution In Controlled Environments, Manal Mutairi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

Comparison of Push-Pull Air Curtain to Overhead Downdraft Systems on Aerosol Containment and Distribution in Controlled Environments.

This dissertation looks at the ongoing issue of tiny airborne particles created during medical and dental procedures and evaluates ways to reduce the risk of exposure for healthcare workers and patients to these particles. The first study focuses on the size, how long particles stay in the air, and how far they travel from common medical procedures that create aerosols, showing that tiny particles (less than 5 µm) can stay in the air long enough to escape basic droplet controls. As a …

Increasing Documentation Of Endotracheal Tube Cuff Pressures: A Quality Improvement Initiative For Flight Crew Providers, Matthew D. Noss

Graduate Theses, Dissertations, and Problem Reports (ETD)

ABSTRACT

Background: There was a lack of documentation of endotracheal tube (ETT) cuff pressures (CPs) by flight crew providers at Base 1 of HealthNet Aeromedical Services. A three-month pre-intervention data collection showed 1.8% of patients had initial CPs documented. Inadequate documentation and monitoring of ETT CPs can lead to patient complications such as tracheal injury and aspiration making this an important area for quality improvement.

Purpose: To increase documentation of ETT CPs through education and evidence-based interventions.

Intervention: This project implemented an educational in-service on ETT cuff syringe manometry for flight crew providers. The educational intervention consisted of …

Mechanisms Of Glyphosate Toxicity: Uptake, Mitochondrial Function, And Metabolism, Dionysios Patriarcheas

Graduate Theses, Dissertations, and Problem Reports (ETD)

Glyphosate, the most widely used herbicide in the world, is an inhibitor of chorismate biosynthesis, the precursor of aromatic amino acids. The target of glyphosate is absent in animals, but findings reporting toxicity in model organisms without a known mechanism or target are increasing. Saccharomyces cerevisiae is a useful and versatile model for understanding molecular mechanisms of toxicity. In yeast, previous work has shown that the aspartate/glutamate permease Dip5 is the proposed transporter for glyphosate and that mitochondrial metabolism has been implicated in response to glyphosate toxicity. This dissertation aimed to identify the effects of glyphosate on mitochondrial function through …

Multi-Omic Characterization Of Immune And Metabolic Responses To Inflammatory Challenges In Sheep, Samanthia Raceine Johnson

Graduate Theses, Dissertations, and Problem Reports (ETD)

To meet the demands of the growing population, it is imperative to develop sustainable and applicable ways to improve animal health and productivity. The first step in doing so is understanding the mechanisms that govern health and immune response and find ways to appropriately modulate them to improve animal health and productivity. The objective of this study is to use multi-omic techniques to understand breed-specific immune responses in sheep that may contribute to their resistance and susceptibility to parasites and to a greater extent, immunocompetence. Known parasite-resistant breed, St. Croix (STC) were compared to parasite susceptible Suffolk (SUF) sheep for …

Video-Based Heart Failure Education: A Feasibility Study, Avery Pivont

Graduate Theses, Dissertations, and Problem Reports (ETD)

Video-Based Heart Failure Education: A Feasibility Study

Avery Pivont

Background:  Heart failure (HF) is a leading cause of hospitalization in the U.S., contributing to high readmission rates and significant healthcare costs. Many of these readmissions are preventable and often stem from inadequate patient education. Effective patient education is a critical component of HF management; however, many healthcare facilities lack specialized HF nurse educators, resulting in inconsistent patient education.

Problem Statement: A quality improvement team at a large academic hospital identified increased heart failure (HF) readmission rates across an entire health system, largely attributed to inconsistent discharge education and limited availability …

Genetics Of Mycangial Development And Host Colonization Within The Ambrosia Symbiosis, Mckeon James Laws

Graduate Theses, Dissertations, and Problem Reports (ETD)

Ambrosia beetles, a polyphyletic group of weevils, utilize pouch-like structures at various locations on the body to transport for their sole food source, their fungal partner. Ambrosia fungi are known to originate from multiple unrelated fungal lineages with no consensus of traits required to become a fungal cultivar. While the biology and ecology of both partners is in many cases well understood, the genetic underpinnings of this fascinating mutualism remain a mystery. The aim of this dissertation is to add to the understanding of the ambrosia symbiosis by providing insights into its molecular and genetic underpinnings through RNA sequencing data, …

Walking In The Footsteps Of My Ancestors: A Deep Map Of Cherokee Forced Removal, Deborah Lyn Kirk

Graduate Theses, Dissertations, and Problem Reports (ETD)

In this study of the place of Cherokee forced removal, I use a Third Space approach to blend Indigenous Methodologies, Deep Mapping, Geospatial mapping and analysis, and 3D Geography. Using a hybrid platform that provides a mélange of media types, storytelling forms, and interactive tools, I reimagine and retell the story of the Cherokee “Trail of Tears.”

In accordance with Cherokee philosophies of duyuktv, harmony, and “a good mind” I first sought out Cherokee sponsorship from community respected and culturally knowledgeable Cherokee citizens; partnered with the National Trail of Tears Association (NTOTA); submitted my research proposal to the Cherokee …

Electronic Cigarettes Increase Blood-Brain And Blood-Tumor Barrier Drug Efflux In A Preclinical Lung Cancer Model Of Brain Metastasis, Cullen Parker Wolford

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation (a) provided a comprehensive review of the known therapeutic challenges in nicotine delivery system users with lung cancer and (b) investigated the impact of electronic cigarettes on blood-brain/blood-tumor barrier function and efficacy of small molecule KRAS inhibitors in a syngeneic lung cancer brain metastasis model. The blood-brain barrier is a physiochemical barrier that limits systemic delivery of therapeutics into brain. When tumors invade the central nervous system, the blood-brain barrier is disrupted and subsequently forms a blood-tumor barrier. Despite the inherent leakiness and variable permeability of the blood-tumor barrier, it continues to restrict anti-cancer drugs from accumulating at …

Evaluation Of Engineering Controls In Mitigation Of Fecal And Chloride Contamination To An Urban Stream, Gabriel J. Zenny

Graduate Theses, Dissertations, and Problem Reports (ETD)

Fecal coliform contamination is a major contributor to surface water pollution in the United States’ (U.S.) waterways, whether from combined sewer overflows (CSOs) (point source pollution), runoff from precipitation and snowmelt (nonpoint source pollution), or exfiltration from aging and failing infrastructure. In addition to fecal bacteria, chloride contamination is also a major, prevalent surface water contaminant impacting U.S. waterways. Chloride contamination is predominantly released into U.S. waterways as nonpoint source pollution, endemic to northern areas that use industrial brine and salt as a form of mitigation for ice accumulation on roadways. The purpose of this research was to investigate impairment …

Managing The Legacy Of Historical Land Use In Central Appalachia: Soil Health, Carbon Storage, And Restoration Across Disturbed Ecosystems, Kyra R. Dukich

Graduate Theses, Dissertations, and Problem Reports (ETD)

Historical mining, intensive timber harvesting, agriculture, and atmospheric acid deposition have left lasting impacts on soil health throughout the Central Appalachian region, reducing soil productivity, carbon storage, and ecosystem resilience. This thesis evaluated restoration strategies across two long-term research systems in West Virginia to determine how land-use history and soil amendments influence soil health, carbon dynamics, and ecosystem recovery. The first study examined reclaimed mine lands and marginal agricultural soils planted with switchgrass (Panicum virgatum) or willow (Salix spp.) and treated with biochar or inorganic fertilizer. Soil greenhouse gas production, aggregate stability, total soil C:N, pH, and soil organic matter …

Factors Influencing Eastern Wild Turkey (Meleagris Gallopavo Silvestris) Productivity And Nesting Chronology In West Virginia, Kirstin Palumbo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Identifying drivers of variation in the timing of breeding behaviors, such as courtship activities and nesting chronology, is important for the effective management of avian species. Likewise, productivity is an important component of game bird population dynamics and can be broken up into multiple components such as the probability of nesting, clutch size, and nest success. One species that experiences a relatively long reproductive season is the Eastern wild turkey (Meleagris gallopavo silvestris). Contemporary research in the southeastern United States has raised concerns regarding declines in both wild turkey populations and overall productivity. West Virginia, however, is unique …

Does Diverse Seeding Adoption Reduce Production Costs? The Case Of Diverse Forage Seeding Strategies In West Virginia., Ivan Andres Wong

Graduate Theses, Dissertations, and Problem Reports (ETD)

Feed and harvested forage represent a major share of production costs in Appalachian cow-calf systems, where a pronounced summer forage gap forces producers to rely on purchased hay. This thesis evaluates whether adopting diverse forage seeding strategies reduces production costs relative to the existing pasture management practice, accounting for the spatial heterogeneity of West Virginia’s production environments. The analysis proceeds in two steps. First, an unsupervised K-means clustering of 14 farms enrolled in the NRCS Conservation Innovation Grant field trial groups the farms into two agroclimatic clusters, Highland-Cool and LowlandWarm, using elevation, growing-season Growing Degree Days, growing-season precipitation, and soil …

Romantic Narrative In Three Early Piano Cycles By Robert Schumann: Papillons, Op. 2; Fantasiestücke, Op. 12; And Kinderszenen, Op. 15, Lingqing Huang

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research project compares Romantic instrumental narrative in Robert Schumann’s Papillons, Op. 2; Fantasiestücke, Op. 12; and Kinderszenen, Op. 15, with particular attention to how pianists communicate cyclic continuity in performance. In this study, narrative continuity refers to relationships formed across ordered short pieces through contrast, interruption, return, literary association, fantasy, memory, tonal and motivic recall, and the shaping of sound and time. Comparative score analysis shows that Schumann creates a distinct form of continuity in each cycle. In Papillons, he creates a public, ballroom-based form through masquerade, waltz-like dance, abrupt entrances, interruption, and the gradual dissolution of the social …

Exploring The Application Of Wastewater Monitoring To Determine The Prevalence And Diversity Of Influenza On The Campus Of West Virginia University, Lakyn Renee Sanders

Graduate Theses, Dissertations, and Problem Reports (ETD)

Influenza viruses (IV) affect populations globally, with the World Health Organization (WHO) estimating that IV are responsible for an estimated 3-5 million infections and 290,000 – 650,000 deaths annually^1–3. More specifically, the 2019-2020 IV season is associated with an estimated 38 million illnesses and 22,000 deaths in the United States alone⁴. IV are rapidly spread between individuals through direct contact with infected individuals, contaminated surfaces, or aerosolized virus particles². Traditional IV infection monitoring relies on clinical screening of patients presenting influenza-like symptoms, commonly through the use of multi-target panel tests such as the BioFire …

Effect Of Delay On Reinforcer Preference And Efficacy, Ciara Jasmine Rodriguez

Graduate Theses, Dissertations, and Problem Reports (ETD)

Behavior intervention plans are used to increase desired behavior and decrease problem behavior, and these plans require the use of effective reinforcers. High-quality reinforcers that are delivered immediately tend to be the most effective, but optimal reinforcement parameters are not always feasible or appropriate in applied settings. Some reinforcers may be disruptive, teachers’ competing responsibilities may introduce delays to reinforcer delivery, and reinforcers may not be immediately accessible. Stimuli may lose their reinforcing value with delay to their receipt, and this is referred to as delay discounting. Thus, there is likely a point where a less preferred but immediately delivered …

Effects Of An Early-Onset Hfd On Hepatic Functioning In A 3xtg Model Of Ad, Matthias Hirst

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Approximately 1 billion people currently live with obesity. Driven by factors such as a high-fat diet (HFD), this pathology can have drastic effects throughout the body, with the liver in particular being a main target of its downstream consequences. Although it is capable of storing lipids, excess fat can conglomerate in the liver and contribute to various hepatic pathologies. A specific pattern of hepatic dysfunction seen in response to an HFD is increased acetylation of mitochondrial proteins. Further, expression and activity of insulin-degrading enzyme (IDE), involved in the degradation of Aβ, are altered in response to an HFD. Aβ …

The Impacts Of Experiential Learning On Moral Development, Jacob Tyler Greenfield

Graduate Theses, Dissertations, and Problem Reports (ETD)

Burnout remains a widely studied phenomenon in the healthcare literature, with much of the focus placed on reactive mitigation strategies that lack meaningful engagement with moral development. Healthcare education programs rarely incorporate formal learning experiences that promote moral development as a means of addressing burnout symptoms. This qualitative single case study examined how health professions students describe the impact of experiential learning on their moral development. Participants engaged in adapted photovoice methods, focus groups, and interviews to reflect on how serving as a Project MUSHROOM student leader influenced their morality. Four themes emerged: 1) recognizing bias and the need to …

Gcn5l1-Mediated Lysine Acetylation Regulates Mitochondrial Dysfunction In The Aged Heart, Jackson Edmond Stewart

Graduate Theses, Dissertations, and Problem Reports (ETD)

For over a century, cardiovascular disease (CVD) has been and remains the leading cause of death globally. CVD risk and severity increase significantly with advanced age, and mitochondrial dysfunction has been implicated in the pathogenesis of aging. Furthermore, mitochondrial health is crucial for cardiac function, as one-third of total cardiomyocyte volume is occupied by mitochondria in order to meet the high energy demand of contractile and relaxational function. It is thus crucial that we understand the pathological mechanisms driving mitochondrial dysfunction in aging and how this contributes to age-associated cardiovascular disease. The current manuscript aims to fill this knowledge gap …

The Effects Of Gesture And Age On Jurors’ Perceptions Of Child Witnesses, Jihan Hani Rashed

Graduate Theses, Dissertations, and Problem Reports (ETD)

Jurors’ perceptions of child witnesses can have significant influence in judicial outcomes, particularly in cases where children’s testimony is central (Goodman et al., 1997; Goodman et al., 1998; Castelli et al., 2005). The role of gesture in shaping jurors’ perceptions is underexplored. Studies highlight that gesture enhances memory recall and reduces cognitive load, making it an important component of effective communication (Goldin-Meadow et al., 2001; Hudson & Sheffield, 1998; Cook & Fenn, 2017). The present research consisted of two studies examining whether child witnesses’ use of gesture during recall of an alleged crime affected jurors’ perceptions of witness credibility and …

Pbmc Transcriptome Analysis Following Lps Stimulation Reveals Distinct Inflammatory Strategies In Feed-Efficient And Inefficient Beef Cattle, Yarahy Leal

Graduate Theses, Dissertations, and Problem Reports (ETD)

Improving feed efficiency is a primary objective in livestock production, serving as a major driver of profitability and sustainability in beef cattle operations as feed costs continue to rise. Residual feed intake (RFI) and residual average daily gain (RADG) are widely recognized indicators of feed efficiency and are commonly used as selection traits to reduce production costs and environmental impact. Previous research suggests a functional shift in transcriptional activity associated with immunity depending on efficiency phenotype, with evidence of greater immune system robustness in more efficient animals. This dissertation examines the molecular mechanisms linking feed efficiency and immune competence and …

Early Life Adversity And Reward Reactivity In Humans, Maria C. Potts

Graduate Theses, Dissertations, and Problem Reports (ETD)

Both animal and human research has identified early life adversity (ELA) as a prominent risk factor for substance use disorder (SUD). However, the underlying mechanisms linking ELA to SUD development remain unclear. Animal studies point to differences in reward responsiveness as the primary explanation; specifically, that ELA could lead to individual differences in the attribution of incentive salience to reward-predictive cues, or the “sign-tracking” behavioral phenotype. Sign-tracking has been linked to addiction-related behaviors in both animal and human studies, but no human studies have examined whether sign-tracking could partially explain the link between ELA and SUD. In fact, human studies …

The Role Of Temperature And Carbon Source In Nutrient Acquisition For Peptidoglycan Synthesis In A Gfp-Expressing Borreliella Burgdorferi, Barrett-Anne Conner Briggs

Graduate Theses, Dissertations, and Problem Reports (ETD)

Lyme disease is the most common vector-borne disease in the United States with an estimated yearly incidence rate of more than 300,000 cases. Borreliella burgdorferi, the etiological agent of Lyme disease, is transmitted by the bite of an infected Ixodes scapularis tick and can cause debilitating pathologies affecting numerous body systems. Symptoms include fever, fatigue, joint pain, and a characteristic bull’s eye rash (erythema migrans) at the site of the tick bite. The timely and appropriate use of antibiotics effectively treats early Lyme disease; however, uncomfortable side effects reduce treatment compliance. If left untreated, long-term complications can arise including facial …

Programs Of Support For Wardship Experienced Students: Exploring Program Directors' Expectations And Experiences Through A Bioecological Framework, Adalheid Faith Crum

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this research is to explore the expectations and experiences of program directors of campus support programs which center wardship experienced students. This research question guided the current study: What expectations and experiences influenced program directors’ development and reform of a wardship-centered campus support program? This inquiry applies Ürie Bronfenbrenner’s Person–Process–Context–Time (PPCT) bioecological model. Semi-structured interviewing was utilized for this case study. In this research, six directors of campus support programs centering wardship experienced students were interviewed. Additionally, this work employs content analysis of twenty-eight National Research Collaborative for Foster Alumni and Higher Education (NRC-FAHE) publications, introducing fourteen …

Optimization Of Feed Manufacturing Productive Capacity, Feed Quality, And Feed Safety Utilizing A Variety Of Pellet Die Parameters, Feed Additives, Conditioning Specifications, And Simulated Feed Bin Storages, Lucas Edward Knarr

Graduate Theses, Dissertations, and Problem Reports (ETD)

In Chapter 2, an experiment was conducted to determine if the inclusion of 1.50% mixer-added water (MAW) and/or 0.25% Azomite^® (AZM) would be suitable in alleviating concerns regarding pellet production rate and pellet quality when manufacturing feed with low-moisture corn. Furthermore, the authors aimed to determine if these additives had varying effects when utilizing different pellet die thicknesses. A 2 (Azomite; 0.00% or 0.25%) x 2 (mixer-added water; 0.00% or 1.50%) x 3 (pellet die thickness; 32 mm, 38 mm, or 45 mm) factorial arrangement was utilized to create the twelve experimental treatments, each manufactured …

Orogenesis, Timothy Ryan Butcher

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis and accompanying exhibition explore the translation of panenhenic mystical experience and the collaboration with Appalachian natural spaces into abstracted landscape images. This work aims to “map” a sensation of fundamental unity and gesture toward the ineffable by utilizing found-object stamp printing, and automatic mark-making. Inspired by practical mystic traditions and contemporary landscape painting, this body of work, entitled Orogenesis, combines intricate, abstracted ink landscape drawings and photographs to create images suggestive of the mystical patterns in the process.