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Genetics And Applications Of Nisin Production In Lactococcus Lactis Subsp. Lactis And Conjugal Exchange Of This Trait, Jeffery R. Broadbent

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Chapter I reviews current literature on gene transfer systems in lactic acid bacteria, how genetically altered microorganisms for food are presently regulated, and how nisin is used as a food preservative.

Chapter II investigates previous reports which linked genes for nisin biosynthesis and sucrose utilization (Nip⁺Suc⁺) to plasmid DNA in two well characterized L. lactis subsp. lactis strains. Plasmid curing studies, conjugations, and DNA-DNA hybridizations indicated that these genes were encoded by chromosomal loci in all Nip⁺Suc⁺ strains examined. Similar results were noted in nisin-sucrose transconjugants of L. lactis subsp. cremoris and S. …

Influence Of Process Parameters In The Manufacture Of Cottage Cheese Curd From Ultrafiltered Skim Milk, Ronald Michael Raynes

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A processing procedure for the manufacture of directly acidified cottage cheese curd from 16% total solids ultrafiltered retentate was developed. The effects of preacidification of skim milk before ultrafiltration and heat treatment of retentate were investigated to improve the functional and sensory qualities of retentate curd.

Retentate directly acidified with phosphoric acid and glucono-delta-lactone to pH 4.7 formed a dense, rubbery curd that could not be handled well in a vat. A heat treatment of 71.1°C for 30 min applied to the retentate resulted in a curd with acceptable handling properties. However, this heat treatment caused the curd to shift …

Effects Of Potassium Lactate, Encapsulated Citric Acid And Storage Temperature On Microbial Growth And Shelf Life Of Pork Sticks, Yen-Kan Su

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A new product, pork sticks, was developed. Optimum shelf life and safety were major concerns associated with this product. Potassium lactate (3%) or citric acid (0.5%, 0.56%, 0.60% or 0.66%) was added to pork sticks to determine their effects on microbial growth, sensory evaluation, and shelf life when stored frozen (-20°C), refrigerated (2°C), or at room temperature (22°C). Two raw materials, pork blade meat (shoulder meat; 91% lean, 9% fat) and regular 80:20 pork trim (80% lean, 20% fat) were used. The consumer panel preferred lean pork sticks made from blade meat over high-fat pork sticks made from regular 80:20 …

Properties Of Low-Fat Yogurt Made From Ultrafiltered And Ultra-High Temperature Treated Milk, Richard Alan Dargan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Yogurts were made from intermediate-high temperature (100, 110, 120, and 130°C for 4 or 16 s), ultra-high temperature (140°C for 4 or 16 s), and vat heat (82°C for 20 min) treatments of skim milk fortified to 5% protein by either ultrafiltration or the addition of nonfat dry milk (NDM). Whey protein denaturation in heated milks increased with temperature and holding time from indirect plate heating and was highest in vat-heated milks. Whey protein denaturation and yogurt water-holding capacity increased with protein levels in the fortified milks compared to skim milk. Penetrometer gel strength and stirred viscosity in 21 day-old …

Estimation Of Selected Milk Protein Genetic Variants By Multi-Component Analysis Of Amino Acid Profiles, Carol M. Hollar

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Cation-exchange fast protein liquid chromatography separated whole casein into β-casein A², A¹, and B, K-casein, α_s1-casein, and α_s2-casein fractions as well as γ-caseins and several unidentified peaks using a urea-acetate buffer at pH 5 and a NaCl gradient. The whole casein fractions eluted in the following order: breakdown products of β-casein and unidentified peaks; β-casein A², A^l, and B; additional breakdown products of β-casein and unidentified peaks; K-casein; α_s1-casein; and α_s2-casein. The calculated composition of the four major caseins correlated well with values obtained …

Ecological Implications Of Flavor Generalization By Sheep: Role Of Flavor Intensity And Variation In Toxin Dose, Karen L. Launchbaugh

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Researchers studying diet selection of ungulate herbivores have generally considered plant palatability independent of animals' dietary history. However, more recent studies demonstrate that experiences within the life of an animal strongly influence plant selection. We are beginning to understand how food preferences and aversions are formed through gastro-intestinal feedback. My research specifically examines factors that influence the formation of conditioned flavor aversions in the generalist herbivore, sheep.

I first examined how variability of food toxicity affects the intake of those foods. I determined that sheep apparently have several mechanisms for regulating intake of toxic foods regardless of whether or not …

The Effects Of Feeding A High Level Of Rumen Protected Fat With Rumen Undegradable Protein With Or Without Niacin On Rumen Fermentation Characteristics, Apparent Nutrient Digestibility, And Milk Production In The Early To Mid Lactation Holstein Cow, Carlos Eduardo Batallas

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Forty high producing early to mid lactation Holstein cows were blocked according to stage of lactation and previous two-week milk yield (experiment 1). Eight ruminally and duodenally cannulated nonlactating Holstein cows were used for experiment 2. The objective was to determine the effects of the ration's high fat content (11.53%) when rumen degradable or rumen undegradable protein is fed with or without added niacin. Cows received one of five treatments: 1) basal ration (TMR); 2) basal ration with added rumen undegradable fat (RUF) (1.6 kg); soybean meal (SBM) (1.73 kg), and niacin (12 g); 3) same as treatment 2, without …

Comparative Economics Of Cattle And Wildlife Ranching In The Zimbabwe Midlands, Urs P. Kreuter

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The economics of ranches in the Zimbabwe Midlands, generating income from cattle, or wildlife, or both, were compared during 1989/90 to test the claim that wildlife ranching can generate greater profits than cattle ranching on semi-arid African savannas. Both financial (market) prices and economic prices (opportunity cost) were used.

Financial data were obtained from 15 cattle, 7 wildlife and 13 mixed ranches in four areas with wildlife and from 15 cattle ranches in two areas with sparse wildlife. Estimates of economic prices were obtained from official data.

In the first paper, gross revenues, costs, net revenues, and capital investments of …

Optimization Of Ranch Management Alternatives In Utah, Scott G. Evans

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to determine the optimum combination of various cattle production, range forage, and crop harvesting alternatives available to ranch owners and managers in Utah. While many promising alternatives are available, determining which alternatives to implement is difficult because the total ranch operation must be considered.

Linear programming (LP) is a tool available to ranch managers which allows the profit maximizing combination of improvements to be easily determined. LP allows ranchers to examine the entire ranch operation and to reduce the amount of risk and uncertainty involved in the decision making process.

The typical Utah ranch …

Marten Use Of Subnivean Access Points In Yellowstone National Park, Wyoming, Stuart Scott Sherburne

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Subnivean prey appeared to be the primary reason for subnivean access point use by martens. A logistic regression was used to create a predictive model for differential access point use. Prey biomass in grams and percent ground cover of coarse woody debris (CWD) were used as variables in the model. Goodness of fit of the multivariate model was 0.216; biomass was significant al p = 0.0003, CWD was significant at p = 0.0718. Mean values for prey biomass at used and unused access points were 174.2 g and 81 g, respectively, while mean values of CWD were 24.7% and 18.5%, …

Soil Heterogeneity And Its Exploitation By Plants, Robert B. Jackson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In this dissertation I first examine the ability of individual plants in the field to garner localized soil nutrients. I then measure actual soil variability around perennial plants and use various statistics to quantify the scale and degree of that variability.

Soil patches on opposite sides of Pseudoroegneria spicata tussocks were treated with distilled water or a nutrient solution containing N, P, or K in three field experiments. When P was augmented in the enriched soil patches, rates of P uptake increased significantly for roots from enriched patches compared with roots in control patches. Rates of ammonium and potassium uptake …

Characteristics Related To The Incidence Of Osteoporosis In Two Distinct Female Populations, Deborah A. Pyke

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Osteoporosis is responsible for approximately 1.3 to 1.5 million fractures per year in the United States. The risk of osteoporosis increases with age, especially among postmenopausal women, and with lifestyle factors such as the use of certain drugs, heavy alcohol consumption, cigarette smoking, high caffeine intake, and sedentary living. Genetic factors that may influence susceptibility to osteoporosis include a positive family history of the disease, or a low weight - for-height ratio. In some cases, race and geographic location may increase or decrease the risk of osteoporosis. Known protective factors include obesity, estrogen replacement therapy, weight bearing exercise, and possibly …

The Influence Of Dietary Factors On The Risk Of Urinary Stone Formation, A. Hesse, R. Siener, H. Heynck, A. Jahnen

Scanning Microscopy

The action of various beverages and foods on the composition of the urine in the circadian rhythm and in the 24-hour urine has been investigated under standardized conditions. Orange juice leads to a significant increase of urinary pH and citric acid excretion. Black tea leads to a raised excretion of oxalic acid by only 7.9%. In the short term, beer increases diuresis, but afterwards leads to a compensatory antidiuresis with increased risk of stone formation. Depending on their composition, mineral waters have very different effects on the urinary constituents. Milk as well as cocoa beverage significantly increase calcium excretion; moreover, …

Utah Science Vol. 53 No. 1, Spring 1992

Utah Science

3 Preparing Students for the 21st Century

11 Welcoming New Students and New Ideas

13 Research in Brief

23 Hands ...for a Hands-On Education

26 Concurrent Credit Enrollment in Agriculture: The Evidence is Mixed

29 Increasing the Department's Role in Research

The Use Of Animal Behavior As A Tool For Biological Control, Melissa Biscornet

Undergraduate Honors Capstone Projects

Animal behavior, the way that an animal acts, is a combination of instinctive traits and learned responses to particular situations (Robinson and Bolen 1984). Behavior in young animals involves several different learning mechanisms, including the following: generalization, social facilitation, neophobia, cue-consequence specificity, and harvesting skills. Preferences instilled in young animals through learning can influence diet selection, grazing patterns, and habitat selection later in life (Squibb et al. 1990)

Summer Salad Recipes, Utah State University Extension

All Archived Publications

No abstract provided.

1991-1992 Annual Report, Various Authors

Annual Reports

No abstract provided.

Molecular Strategies To Improve Protein Quality And Reduce Flatulence In Legumes: A Review, Benito O. De Lumen

Food Structure

The application of recombinant DNA and related techniques to plants opened up the potential to improve agronomic characters. food processing traits and food quality properties of plants as food sources. This review focuses on molecular approaches to improving the protein quality and reducing flatulence in beans. Much is now known about the regulation of seed-specific expression and organization of seed storage protein genes. Several attempts have been made to increase the methionine content of tobacco seeds using heterologous seed protein genes from peas, common bean and soybean. Such techniques could also be used to modify lysine, tryptophan and threonine contents …

Importance Of Enzymes To Value-Added Quality Of Foods, J. R. Whitaker

Food Structure

New uses of enzymes are of major importance for value-added quality of foods. At the production and postharvest handling levels, enzymes associated with maturation , color , texture, flavor and nutritional changes are important. Recent examples are discussed which include longer storage prior to initiation of ripening , deletion or control of genes that result in softening1 browning and other quality defects and the enhancement of genes that improve the nutritional quality of foods. At the processing level, new uses of enzymes are described to change the properties of proteins and lipids , to eliminate off-flavor in beers and sterilized …

Scanning Electron Microscopy And Ultraviolet Absorption Microspectrophotometry Of Plant Cell Types Of Different Biodegradabilities, D. E. Akin, L. L. Rigsby

Food Structure

Phenolic components within cell walls are the greatest limitation to biodegradation of plants, but the location and types of compounds within specific walls are poorly understood . Leaf blades of warm-season (Coastal and Coastcross-1 bermuda grasses and sudangrass) and cool-season grasses (orchardgrass and tall fescue) and leaflets of legumes (alfalfa and lespedeza) were evaluated before and after incubation with rumen microorganisms for 7 days to determine cell types and wall structures most resistant to biodegradation. UV absorption microspectrophotometry was used to characterize aromatics within selected undegraded cell types, which had been shown in digestion studies to vary in biodegradability, from …

Structure Of Margarines Made With Low Erucic Acid Rapeseed Oil, J. Hojerova, S. Schmidt, J. Krempasky

Food Structure

Margarine based on hydrogenated low erucic acid rapeseed oil shows a strong tendency to produce an unacceptable grainy structure. This is caused by rapid transition of the B-form into the B-form of fat crystals. Sorbitan esters, monoacylglycerols, and a blend of acylglycerols were investigated as possible B-stabilizers. Polymorphic transitions were evaluated by visual assessment, examination by polarized light microscopy, and x-ray diffraction analysis in margarines prepared on a laboratory scale and containing varying amounts of stabilizers. The evaluation was extended over a 7-week period of storage at 10 C. Sorbitan esters of fatty acids were found to be the most …

Microstructural Differences Among Adzuki Bean (Vigna Angularis) Cultivars, Anup Engquist, Barry G. Swanson

Food Structure

Scanning electron microscopy (SEM) was used to study microstructural differences among five adzuki bean cultivars: Erimo, Express, Hatsune, Takara and VBSC. Seed coat surfaces showed different patterns of cracks, pits and deposits . Cross-sections of the seed coats revealed well organized layers of elongated palisade cells followed by many layers of amorphous parenchyma cells. Typical sub-epidermal layers of organized columnar, hour-glass cells were characteristica11y absent in the five cultivars of adzuki beans. The cross-sections of the cotyledons revealed intercellular materials between storage cells. Storage cells in Hatsune cultivar were loosely packed with many intercellular spaces as compared to the other …

Scanning Electron Microscopy Structure And Firmness Of Papain Treated Apple Slices, Yaguang Luo, Max E. Patterson, Barry G. Swanson

Food Structure

'Mcintosh' apple (Malus domesrica Borkh.) slices were treated with papain. Textural changes were recorded with an Instron Universal Testing Machine. Structural changes and distribution of microorganisms in apple tissues after treatment were observed with a scanning electron microscope (SEM). Apple slices submerg ed in a 1% papain solution were significantly firmer than apple slices submerged in the distilled water control for a 24 hour period (P < 0.05). Three and four days after slicing , a significantly smaller decay index was observed on the apple slices submerged in papain solution than on the control slices. Under SEM, less severe cell wall breakdown was observed on the apple tissues treated with papain than on apple tissues without treatment. Less spores were also observed on the papain treated apple slices than apple slices without treatment. Apple tissues treated with papain solution and distilled water also demonstrated noticeable st ru ctural differences. The apple tissues treated with papain solution for 18 hours retained the original cell structure while the cells in the apple tissues treated with distilled water collapsed.

Clinical Applications Of Recombinant Gene Technology: Bone And Cartilage Repair, Thomas A. Einhorn

Cells and Materials

Over the past decade, the field of molecular biology has given rise to the development of the applied discipline of molecular medicine. Based on recent developments using recombinant gene technology, genetic mapping analysis and other investigational tools, the physician and surgeon is now ready to begin using those instruments in the diagnosis and treatment of musculoskeletal disease. This article reviews the history of scientific exploration in recombinant gene technology as it applies to bone and cartilage repair. Clinical cases are presented to show how the fruits of scientific knowledge may be brought to bear on some of the more challenging …

Cheese Industry Conference 1992, Various Authors

Cheese Industry Conference

No abstract provided.

Ingredient Substitution, Georgia C. Lauritzen

All Archived Publications

No abstract provided.

Whole Kernel And Bulgur Wheat Preparation And Usage, Georgia C. Lauritzen, Charlotte P. Brennand, Deloy G. Hendricks

All Archived Publications

No abstract provided.

Storage Of Dry Milk, Charlotte P. Brennand

All Archived Publications

No abstract provided.

What Is Protein, Georgia C. Lauritzen

All Archived Publications

No abstract provided.

Reduced Sugar And Sugar-Free Food Preservation, Georgia C. Lauritzen

All Archived Publications

No abstract provided.