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Posttranslational Regulation Of Phosphoenolpyruvate Carboxylase In C4 And Crassulacean Acid Metabolism Plants, Jin-An Jiao, Raymond Chollet

Department of Biochemistry: Faculty Publications

Control of C₄ photosynthesis and Crassulacean acid metabolism (CAM) is, in part, mediated by the diel regulation of phosphoenolpyruvate carboxylase (PEPC) activity. The nature of this regulation of PEPC in the leaf cell cytoplasm of C₄ and CAM plants is both metabolite-related and posttranslational. Specifically, the regulatory properties of the enzyme vary in accord with the physiological activity of C₄ photosynthesis and CAM: PEPC is less sensitive to feedback inhibition by L-malate under light (C₄ plants) or at night (CAM plants) than in darkness (C₄) or during the day (CAM). While the view that …

In Vivo Regulatory Phosphorylation Site In C4-Leaf Phosphoenolpyruvate Carboxylase From Maize And Sorghum, Jin-An Jiao, Jean Vidal, Cristina Echevarría, Raymond Chollet

Department of Biochemistry: Faculty Publications

Reversible seryl-phosphorylation contributes to the light/dark regulation of C₄-leaf phosphoenolpyruvate carboxylase (PEPC) activity in vivo. The specific regulatory residue that, upon in vitro phosphorylation by a maize-leaf protein-serine kinase(s), leads to an increase in catalytic activity and a decrease in malatesensitivity of the target enzyme has been recently identified as Ser-15 in ³²P-phosphorylated/activated dark-form maize PEPC (J-A Jiao, R Chollet [1990] Arch Biochem Biophys 283: 300-305). In order to ascertain whether this N-terminal seryl residue is, indeed, the in vivo regulatory phosphorylation site, [³²P]phosphopeptides were isolated and purified from in vivo ³²P-labeled maize and …

Mitochondrial Malate Dehydrogenase From Corn, Marianne K. Hayes, Michael Hans Luethy, Thomas Elthon

School of Biological Sciences: Faculty Publications

A method to fractionate com (Zea mays L. B73) mitochondria into soluble proteins, high molecular weight soluble proteins, and membrane proteins was developed. These fractions were analyzed by both sodium dodecyl sulfate-polyacrylamide gel electrophoresis and assays of mitochondrial enzyme activities. The Krebs cycle enzymes were enriched in the soluble fraction. Malate dehydrogenase has been purified from the soluble fraction by a two-step fast protein liquid chromatography method. Six different malate dehydrogenase peaks were obtained from the Mono 0 column. These peaks were individually purified using a Phenyl Superose column. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified peaks showed that …

Eumeces Septentrionalis (Prairie Skink): Piscivory., Louis A. Somma

Papers in Herpetology

The prairie skink is a North American lizard characterized by a diet that is primarily insectivorous (Breckenridge 1943. Amer. MidI. Nat. 29:591-606). Fish have not been previously recorded as a food item (reviewed in Somma and Cochran 1989. Great Basin Nat. 49:525-534). On 3 June 1990 between 0930 and 1030 h, six Eumeces septentrionalis (three gravid females, one male, and two juveniles) were captured from amongst riprap and beneath stone slabs on the earthen dam forming the windward shoreline of Burchard lake, Pawnee Co., Nebraska (T.12N., R.10E., Sec. 4). The three females and one juvenile were placed in the …

Herbivore-Plant Interaction: Temperate And Tropical Patterns: Review Of Peter W. Price Et Al., Eds., Plant-Animal Interactions: Evolutionary Ecology In Tropical And Temperate Regions, Svata M. Louda

Svata M. Louda Publications

This book is an interesting compendium of old and new thoughts on the interactions of herbivores, especially insect herbivores, with plants; the title, however, seems a bit more ambitious. Pollination, for example, surely an important "plant-animal interaction" in both temperate and tropical regions, is mentioned only in passing. This most likely reflects the book's origins in an international symposium on the evolutionary ecology of tropical herbivores. As usual in such a collection of papers, the papers vary in purpose, length, and new insights; even papers that are reviews of previously published information, however, seem to me to present the authors' …

Detergent (Sodium Dodecyl Sulfate) Shock Proteins In Escherichia Coli, Michael Adamowicz, Philip M. Kelley, Kenneth W. Nickerson

Papers in Microbiology

The protein composition of Escherichia coli W3110 grown in the presence and absence of 5% sodium dodecyl sulfate (SDS) was examined by two-dimensional gel electrophoresis. In SDS-grown cells, at least 4 proteins were turned on, 13 were turned off, 15 were elevated, and 15 were depressed. The 19 unique and elevated SDS-induced spots constituted 7.91% of the total ³⁵S-labeled protein. There was no apparent overlap between these 19 detergent (SDS) stress proteins and those of other known bacterial stress responses. The detergent stress stimulon is a distinct and independent stimulon. Its physiological relevance probably derives from the presence of …

Early Development Of Larval Taenia Polyacantha In Experimental Intermediate Hosts, Osamu Fujita, Yuzaburo Oku, Munehiro Okamoto, Hiroshi Sato, Hong Kean Ooi, Masao Kamiya, Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The early larval development and migration route of Taenia polyacantha were examined using oral inoculation of oncospheres into red-backed voles and Mongolian gerbils. The larvae were recovered in the wall of the small intestine and in the mesenteric lymph nodes by 5 days postinfection (PI) and from the peritoneal cavity after 6 days PI. These results suggest that the larval cestodes developed initially in the wall of the small intestine and the mesenteric lymph nodes, and later migrated to the peritoneal cavity. Although the development of the parasite was quite similar in the 2 host species, pathological changes were different. …

Génétique Systematique Et Des Trichinella [Table Ronde] = Genetics And Systematics Of Trichinella [Round Table], K. D. Murrell, J. Ralph Lichtenfels, Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Up to the present time, the existence of two species in the genus Trichinella has been accepted by most workers. The proliferation of isolates and the definition of numerous genetic populations have caused considerable confusion in recent years for taxonomists and others concerned with these nematodes. The application of the biological species-concept to nematodes of the genus Trichinella has been difficult, but the recent investigations of Dr. E. Pozio and coworkers at the Trichinella Reference Center (Laboratory of Parasitology, Istituto Superiore di Sanita, viale Regina Elena, 299-00161 Rome, Italy) have provided new information on numerous isolates from localities worldwide. By …

Lists Of Larval Worms From Marine Invertebrates Of The Pacific Coast Of North America, Hilda Lei Ching

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Immature stages of seventy-three digenetic trematodes are listed by their families, marine invertebrate hosts, and localities and then cross listed according to their molluscan hosts. The list contains many new host records, and larval stages of fifteen digenetic trematodes were newly recorded from gastropods from British Columbia and California. A list of immature stages of fourteen cestodes, acanthocephaIans, and nematodes is also included.

Sterol Composition Of The Corn Root Lesion Nematode, Pratylenchus Agilis, And Corn Root Cultures, David J. Chitwood, William R. Lusby

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Sterols from mixed stages of the com root lesion nematode, Pratylenchus agilis, and uninfected corn root cultures were analyzed by gas chromatography-mass spectrometry. Twenty-eight sterols were identified in P. agilis, including nine not previously detected in nematodes. The major sterols were 24-ethylcholest-22-enol, 24-ethylcholesta-5,22-dienol, 24-methy1cholestanol, 24-ethylcholestanol, isofucostanol, and 24-ethylcholesterol. The principal corn root sterols were 24-ethylcholesta-5,22-dienol, 24-methylcholesterol, 24-ethylcholesterol, isofucosterol, and cycloartenol. Therefore, the major metabolic transformation of sterols by P. agilis was saturation of the sterol nucleus. In addition, very small amounts of 4α-methylsterols were biosynthesized by P. agilis. The 4-methylation pathway is unique to nematodes and was …

Rickettsial And Mollicute Infections In Hepatopancreatic Cells Of Cultured Pacific White Shrimp (Penaeus Vannamei), Rena M. Krol, William E. Hawkins, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Infections by multiple species of bacteria occurred in hepatopancreatic epithelial cells of cultured Pacific white shrimp (Penaeus vannamei). Grossly, hepatopancreases of moribund shrimp were pale white. Light microscopically, hepatopancreatic tubules appeared atrophied and were associated with granulomas. Examination by scanning and transmission electron microscopy revealed heavy cytoplasmic infections by three forms of microorganisms: (1) a rickettsia-like bacterium, (2) a helical form of a mollicute-like bacterium, and (3) a filamentous mollicute-like bacterium. The rod-shaped rickettsia (900 nm long by 300 nm wide) appeared to be free in the cytoplasm and had both a plasma membrane and a cell wall. …

On The Normalized Yield (Events/Rad/Dalton) Of Biological Molecules Irradiated With Energetic Heavy Ions, Robert Katz

Robert Katz Publications

When irradiated with electrons or γ-rays, a number of biological molecules display an approximately linear relationship between the reciprocal of the D₃₇ dose and molecular weight. This is conventionally plotted as a relation between target molecular weight and true molecular weight, where the target molecular weight M_t is given as: M_t × (D₃₇) = α (1) where α is approximately 10¹⁰ Da-rad/eV of the energy assumed to be “deposited in the target” in a single “hit” for expression of the end-point. We emphasize that D₃₇ is expressed in rads for we will later …

Radial Distribution Of Dose, Robert Katz, Matesh N. Varma

Robert Katz Publications

The radial distribution of dose about the path of a heavy ion, principally from delta rays, is one of the central contributions of atomic physics to the systematization of high LET radiation effects in condensed matter, whether the detection arises in chemical, physical, or biological systems. In addition to the radial distribution of dose, we require knowledge of the response of the system to X-rays or gamma-rays or to beams of energetic electrons such that the electron slowing-down spectra from these radiations can approximate the slowing-down spectra from delta rays even at different radial distances from the ion's path. A …

Cross Sections For Single And Double Strand Breaks In Sv-40 Virus In Eo Buffer After Heavy Ion Irradiation: Experiment And Theory, Robert Katz, S. Wesely

Robert Katz Publications

Measured cross sections after heavy ion bombardment, for both single and double strand breaks of SV-40 virus in EO buffer (which emphasizes indirect effects), are consistent with the theory of Butts and Katz for 1-hit detectors.

Parenting Factors, Social Skills, And Value Commitments As Precursors To School Failure, Involvement With Deviant Peers, And Delinquent Behavior, Ronald L. Simons, Les B. Whitbeck, Rand D. Conger, Katherine J. Conger

Department of Sociology: Faculty Publications

Elements of social control theory were combined with social learning theory to construct a model of delinquency which specifies the manner in which parenting factors, social skills, value commitments, and problems in school contribute to association with deviant peers and involvement in delinquent behavior. The model was tested using a sample of 61 families, each of which included a seventh grader. Questionnaire responses and coded videotaped family interaction were employed as measures of study constructs. The results largely supported the proposed model.

Potential Impacts Of Biomass Production In The United States On Biological Diversity, James H. Cook, Jan Beyea, Kathleen H. Keeler

School of Biological Sciences: Faculty Publications

Biomass could be a renewable source of energy and chemicals that would not add CO₂ to the atmosphere. It will become economically competitive as its cost decreases relative to energy costs, and biotechnology is expected to accelerate this trend by increasing biomass productivity. Pressure to slow global warming may also make biomass more attractive. Substantial dependence on biomass would entail massive changes in land use, risking serious reductions in biodiversity through destruction of habitat for native species. Forests could be managed and harvested more intensively, and virtually all arable land unsuitable for high-value agriculture or silviculture might be used …

Origin And Evolution Of Defective Interfering Rnas Of Tomato Bushy Stunt Virus, David A. Knorr, Thomas Jack Morris

School of Biological Sciences: Faculty Publications

Viruses with defective genomes have been identified in association with virtually every major family of viruses and have been widely utilized as tools for investigating virus functions in animal cell culture systems (Perrault, 1981). It is generally thought that defective interfering viruses (DIs) arise through deletion, rearrangement, or recombination of a competent viral genome. DIs tack the ability for independent existence relying on their parental helper viruses to supply factors required for replication, maturation, and/or encapsidation (Huang and Baltimore, 1977). The interference attributed to DIs is thought to result from competition with the helper virus for factors required in trans …

Dietary And Parasitological Analysis Of Coprolites Recovered From Mummy 5, Ventana Cave, Arizona, Karl Reinhard, Richard H. Hevly

Karl Reinhard Publications

Four coprolites were excavated with Burial 5 at Ventana Cave. a partially mummified five-year-old child. Two coprolites were granular and dark in color and two were fibrous and light in color. The coprolites are remains of the child's intestinal contents and were submitted for dietary and parasitological analysis. No parasites were found. The fibrous coprolites proved to be remains of highly masticated mesquite pods (Prosopis). The granular coprolites consist of seeds of saguaro cactus (Carnegiea gigantea). Pollen analysis reveals two dietary pollen types, both derived from cactus. No evidence of cultivated plants except for a trace amount of …

Radiation Risk Predictions For Space Station Freedom Orbits, Francis A. Cucinotta, William Atwell, Mark Weyland, Alva C. Hardy, John W. Wilson, Lawrence W. Townsend, Judy L. Shinn, Robert Katz

Robert Katz Publications

Risk-assessment calculations are presented for the preliminary proposed solar minimum and solar maximum orbits for Space Station Freedom (SSF). Integral linear energy transfer (LET) fluence spectra are calculated for the trapped-proton and galactic cosmic ray (GCR) environments. Organ-dose calculations are discussed using the Computerized Anatomical Man model. The cellular track model of Katz is applied to calculate cell survival, transformation, and mutation rates for various aluminum shields. Comparisons between relative biological effectiveness (RBE) and quality factors (QF) for SSF orbits are made, and fluence-dependent effects are discussed.

Alice S. Rossi (1922 – )., Mary Jo Deegan, Michael R. Hill

Department of Sociology: Faculty Publications

Alice S. Rossi is a Renaissance scholar. She is a leader in several specialties within sociology and between sociology and other disciplines. Her studies of women, occupations, the family, the life course, sociobiography, and socialization bring together work and ideas from sociology, biology, history, psychology, and anthropology. Her far-ranging academic interests are matched by her influence in the wider society and within professional circles.

Additional Fossil Evidence On The Differentiation Of The Earliest Euprimates, Kenneth D. Rose, Thomas M. Bown

United States Geological Survey: Staff Publications

Several well-preserved jaws of the rare North American omomyid primate Steinius vespertinus, including the first known antemolar dentitions, have been discovered in 1989 and 1990 in the early Eocene Willwood Formation of the Bighorn Basin, Wyoming. They indicate that its dental formula is as primitive as those in early Eocene Donrussellia (Adapidae) and Teilhardina (Omomyidae) - widely considered to be the most primitive known euprimates - and that in various dental characters Steinius is as primitive or more so than Teilhardina. Therefore, despite its occurrence at least 2 million years later than Teilhardina, S. vespertinus is the …

Redescription Of Eimeria Escomeli (Rastegaieff, 1930) From Myrmecophaga Tridactyla, And A First Report From Bolivia, Scott Lyell Gardner, Steve Upton, C. R. Lambert, O. C. Jordán

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Eimeria escomeli (Rastegaieff, 1930) Levine and Becker, 1933, is redescribed from the giant anteater, Myrmecophaga tridactyla (L.), from the departamento de La Paz, Bolivia. This is the first report of parasites from M. tridactyla from Bolivia and only the third time that coccidians have been recorded from this host.

A Statistical Approach To The Objective Differenciation Of Hirschmanniella Oryzae From H. Belli (Nemata : Pratylenchidae), Renaud Fortuner, Armand R. Maggenti

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Alternate English title: A Statistical Approach to the Objective Differentiation of Hirschmanniella oryzae from H. belli (Nemata : Pratylenchidae)

English language abstract: California populations attributed to Hirschmanniella belli were compared i) to paratypes of this species, ii) to topotypes of H. oryzae, and iii) to other populations of the same genus from other parts of the world. Comparisons were made using discriminant function analyses. Some small differences between California populations and paratypes of H. belli were attributed to artifacts caused by the long storage of these paratypes. Several characters were selected that were not affected by these artifacts, but …

Equipment For Ridge Planting, Elbert C. Dickey, Paul J. Jasa

Department of Agricultural and Biological Systems Engineering: Faculty Publications

In ridge plant systems, crops are planted into ridges formed during cultivation of the previous crop.

Row cleaning devices on the planter push weed seed and residue lying on the soil surface from the ridge to the area between the rows, or row middles. The crop seed is planted into the old row in a cleanly tilled strip at a higher elevation than the row middles. A band application of herbicide behind the planter typically is used in the row for weed control. Crop cultivation controls weeds between the row and rebuilds ridges for the following year.

Ridge plant systems …

The Challenge Of Parasitic Worms, D. W. T. Crompton

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

This is a summary of the Montgomery Lectures for 1990, University of Nebraska-Lincoln. The lectures were funded by the UNL Research Council through an endowment from J. H. Montgomery

The Distribution And Reproductive Phenology Of The Milkweeds (Asclepiadaceae: Asclepias And Cynanchum) In Nebraska, Robert B. Kaul, Steven B. Rolfsmeier, Jeffrey J. Esch

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

The Asclepiadaceae are represented in Nebraska by seventeen native species of Asclepias and one of Cynanchum. Two species are here added to the State's flora: Asclepias asperula and A. purpurascens. Detailed county distribution maps are presented for all species based upon our extensive field and herbarium work, and numerous additions and corrections are made to the maps published in the Atlas of the Flora of the Great Plains in 1977. A map is presented showing the zone of hybridization of A. speciosa with A. syriaca in central Nebraska. Data are given on flowering and fruiting phenology for each …

A Biological Inventory And General Assessment Of Eastern Nebraska Saline Wetlands In Lancaster And Southern Saunders Counties, Richard A. Gersib, Gerald A. Steinauer

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

A recent inventory of Lancaster and southern Saunders counties identified 133 saline wetlands and 99 potential saline wetlands. The wetlands ranged from 0.4 ha (1 ac) to over 80 ha (200 ac). Information was gathered on the quality, threats, vulnerability, restoration feasibility, and water source of each wetland. Saline wetlands in Lancaster and southern Saunders counties have undergone extensive degradation through commercial, residential, and agricultural development. Recommendations for preservation of saline and potential saline wetlands are given.

Pectoral-Spine Embedding To Facilitate Scetioning For Age Analysis Of Young Channel Catfish (Ictalurus Punctatus), Michael T. Kaminski, Edward J. Peters, Richard S. Holland

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

We describe a method of preparing pectoral spines from juvenile channel catfish Ictalurus punctatus and other ictalurid species that allows easy sectioning and aging. The method uses marine epoxy poured into molds that enables the encased spine to be held firmly for sectioning with a saw. The technique costs less than US$10 to prepare up to 500 spines and takes only a few hours

The Fecundity And Reproductive Season Of Fundulus Sciadicus In Nebraska (Actinopterygii: Fundulidae), Todd A. Kinney, John D. Lynch

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

The reproductive season of Fundulus sciadicus in Nebraska was investigated using preserved materials. Ovarian eggs matured in late March or early April. By mid-July all adult females were spent. Females with enlarged eggs were in their second, third, and fourth seasons of life. Eggs were classified on the basis of their morphology and the presence or absence of oil droplets and yolk. "Mature" eggs ranged in size from 1.6 to 2.0 mm in diameter. The largest ovarian complement (mature eggs only) was 88. Evidence of reabsorption (large eggs that have collapsed and have only small amounts of yolk) was seen …

Leeches (Annelida: Hirudinea) In Central And Western Nebraska, William A. Moser

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Sixteen sites in western and central Nebraska were sampled for leeches. Of the ten species found, three are newly recorded in Nebraska: Alboglossiphonia heteroclita, Dina dubia, and Theromyzon biannulatum. The habitats and ecology of each species are described