Visualization Of Intracellular Transport Of Vesicular Stomatitis Virus Nucleocapsids In Living Cells, Subash C. Das, Debasis Nayak, You Zhou, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The phosphoprotein (P) of vesicular stomatitis virus (VSV) is a subunit of the viral RNA polymerase. In previous studies, we demonstrated that insertion of 19 amino acids in the hinge region of the protein had no significant effect on P protein function. In the present study, we inserted full-length enhanced green fluorescent protein (eGFP) in frame into the hinge region of P and show that the fusion protein (PeGFP) is functional in viral genome transcription and replication, albeit with reduced activity. A recombinant vesicular stomatitis virus encoding PeGFP in place of the P protein (VSV-PeGFP), which possessed reduced growth kinetics …

Cross-Subtype T-Cell Immune Responses Induced By A Human Immunodeficiency Virus Type 1 Group M Consensus Env Immunogen†0--, Eric A. Weaver, Zhongjing Lu, Zenaido T. Camacho, Fatiha Moukdar, Hua-Xin Liao, Ben-Jiang Ma, Mark Muldoon, James Theiler, Gary J. Nabel, Norman L. Letvin, Bette T. Korber, Beatrice H. Hahn, Barton F. Haynes, Feng Gao

Nebraska Center for Virology: Faculty Publications

The genetic diversity among globally circulating human immunodeficiency virus type 1 (HIV-1) strains is a serious challenge for HIV-1 vaccine design. We have generated a synthetic groupMconsensus env gene (CON6) for induction of cross-subtype immune responses and report here a comparative study of T-cell responses to this and natural strain env immunogens in a murine model. Three different strains of mice were immunized with CON6 as well as subtype A, B, or C env immunogens, using a DNA prime-recombinant vaccinia virus boost strategy. T-cell epitopes were mapped by gamma interferon enzyme-linked immunospot analysis using five overlapping Env peptide sets from …

Ecological Fitting As A Determinant Of The Community Structure Of Platyhelminth Parasites Of Anurans, Daniel R. Brooks, Virginia León-Règagnon, Deborah A. Mclennan, Derek Zelmer

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Host-parasite associations are assumed to be ecologically specialized, tightly coevolved systems driven by mutual modification in which host switching is a rare phenomenon. Ecological fitting, however, increases the probability of host switching, creating incongruences between host and parasite phylogenies, when (1) specialization on a particular host resource is a shared characteristic of distantly related parasites, and (2) the resource being tracked by the parasite is widespread among many host species. We investigated the effect of ecological fitting on structuring the platyhelminth communities of anurans from a temperate forest and grassland in the United States and tropical dry and wet forests …

Invasive Predators: A Synthesis Of The Past, Present, And Future, William C. Pitt, Gary W. Witmer

United States Department of Agriculture Wildlife Services: Staff Publications

Invasive predators have had devastating effects on species around the world and their effects are increasing. Successful invasive predators typically have a high reproductive rate, short generation times, a generalized diet, and are small or secretive. However, the probability of a successful invasion is also dependent on the qualities of the ecosystem invaded. Ecosystems with a limited assemblage of native species are the most susceptible to invasion provided that habitat and climate are favorable. In addition, the number of invasion opportunities for a species increases the likelihood that the species will successfully establish. The list of routes of entry or …

Career Paths Of Women Clinical Laboratory Scientists Who Have Become Higher Education Administrators, Suzanne Campbell

Department of Educational Administration: Dissertations, Theses, and Student Research

More women are joining their male colleagues as higher education administrators. However, only a small percentage of these women possess an academic background as clinical laboratory scientists. This qualitative case study sought to investigate and document the career paths of women clinical laboratory scientists that have transitioned from the clinical setting to the higher education arena and held an administrative position at the dean’s level, including assistant and associate dean positions.

Through face-to-face semi-formal interviews, the experiences of this purposive sample population were recorded. Areas of inquiry included a description of their career paths; an identification of the skills, training, …

Farmer's Market Food Safety, Dianne Peters

Food Processing Center: Reports

Discussion Topics:

• Food Microbiology Overview
• Acidified Foods
• Hazards of Fresh Fruit and Vegetables

Wildlife Health Centre Newsletter, Volume 12, Spring/Summer 2006

Canadian Cooperative Wildlife Health Centre: Newsletters and Publications

Canada’s Inter-agency Wild Bird Influenza Survey—Results from 2005
Devil’s Lake Pathogen Surveys
Wildlife Health Alert—Adenoviral Hemorrhagic Disease Updates
First Charitable Donation
Download your newsletter from our Website

Wildlife Health Centre Newsletter, Volume 12-1, Spring & Summer 2006

Canadian Cooperative Wildlife Health Centre: Newsletters and Publications

Canada’s Inter-agency Wild Bird Influenza Survey—Results from 2005
Devil’s Lake Pathogen Surveys
Wildlife Health Alert—Adenoviral Hemorrhagic Disease of Deer
Anticoagulant Rodenticide Toxicity in Birds
Salmonellosis Returns to the Maritime Provinces’ Passerine Bird Populations
Skin Disease in Canada Geese
Escherichia albertii in Songbirds on Prince Edward Island
Illegal Shooting of Raptorial Birds in the Province of Quebec from 1986 to 2004
Nocardiosis in a Juvenile Hooded Seal (Cystophora cristata)
Autopsy of the Beluga Whales Necropsy Program: Two Decades of Data
Schistosomiasis-related Mortality in Ring-billed Gulls
Common Toxicological Problems of Ontario Wildlife
Fenthion Poisoning in Black-billed Magpies (Pica pica)
Demodecosis in a …

A New Species Of Myxidium (Myxosporea: Myxidiidae), From The Western Chorus Frog, Pseudacris Triseriata Triseriata, And Blanchard's Cricket Frog, Acris Crepitans Blanchardi (Hylidae), From Eastern Nebraska: Morphology, Phylogeny, And Critical Comments On Amphibian Myxidium Taxonomy, Miloslav Jirků, Matthew G. Bolek, Christopher M. Whipps, John J. Janovy Jr., Mike L. Kent, David Modrý

John J. Janovy, Jr. Publications

During March 2001-April 2004, 164 adult anurans of 6 species (47 Rana blairi, 35 Rana catesbeiana, 31 Hyla chrysoscelis, 31 Pseudacris triseriata triseriata, 11 Bufo woodhousii, and 9 Acris crepitans blanchardi) from Pawnee Lake, Lancaster County, Nebraska, were surveyed for myxozoan parasites. Of these, 20 of 31 (65%) P. triseriata triseriata and 1 of 9 (11%) A. crepitans blanchardi were infected with a new species of Myxidium. Myxidium melleni n. sp. (Myxosporea) is described from the gallbladder of the western chorus frog, P. triseriata triseriata (Hylidae). This is the second species of Myxidium …

Most Popular Downloads -- May 2006,

Institutional Repository Information

The 198 most often downloaded files from the UNL Digital Commons for May 2006. ALso includes statistics and graphs of total repository contents, hits, and downloads for the period July 1, 2005 through May 31, 2006.

Paracreptotrema Blancoi N. Gen., N. Sp. (Digenea: Plagiorchiformes: Allocreadiidae) In The Olomina, Priapichthys Annectens (Osteichthyes: Poeciliidae), From The Área De Conservación Guanacaste, Costa Rica, Anindo Choudhury, Gerardo Pérez Ponce De León, Daniel R. Brooks, Rita Daverdin

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A new species of allocreadiid digenean is described from Priapichthys annectens (Regan) (Osteichthyes: Poeciliidae) in the Área de Conservación Guanacaste, Costa Rica. A new genus, Paracreptotrema is proposed to accommodate this species as well as Fellodistomum mendezi Songandares-Bernal, 1955, which was previously described from another poeciliid, Brachyrhaphis episcopi, in Panama. Paracreptotrema differs from all other nominal genera of Allocreadiidae by a combination of its symmetrical testes, restricted vitellaria, and the lack of oral lappets (muscular 'papillae') or other such appendages. Paracreptotrema blancoi n. sp. resembles Creptotrema creptotrema Travassos, Artigas and Pereira, 1928, but differs in lacking ventral oral lappets …

Disease Emergence And Resurgence: The Wildlife-Human Connection, Milton Friend

Other Publications in Wildlife Management

Humans have been affected by and have contributed to the eruption and spread of disease since antiquity. This connectivity is such that disease in the Americas was one of the five Seeds of Change addressed by the National Museum of Natural History’s commemoration of the Columbus Quincentenary. As for the other Seeds of Change, diseases “…sent ripples around the globe, affecting the people as well as the flora and fauna of both the New World and the Old.” During recent decades, these ripples have become waves that are likely to intensify, swelled by human population growth, civil strife, and other …

Post-Miocene Expansion, Colonization, And Host Switching Drove Speciation Among Extant Nematodes Of The Archaic Genus Trichinella, D. S. Zarlenga, B. M. Rosenthal, G. Larosa, E. Pozio, Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Parasitic nematodes of the genus Trichinella cause significant food-borne illness and occupy a unique evolutionary position at the base of the phylum Nematoda, unlike the free-living nematode Caenorhabditis elegans. Although the forthcoming genome sequence of Trichinella spiralis can provide invaluable comparative information about nematode biology, a basic framework for understanding the history of the genus Trichinella is needed to maximize its utility. We therefore developed the first robust and comprehensive analysis of the phylogeny and biogeographic history of Trichinella using the variation in three genes (nuclear small-subunit rDNA, and second internal transcribed spacer, mitochondrial large-subunit rDNA, and cytochrome oxidase …

Cotton Top Farm: A Natural Horsemanship Training Facility, Nicole Schiermeyer

Architecture Masters of Science Program: Theses

In order to meet the requirements of the masters of Architecture Terminal Project, I propose to design an equestrian training facility for Cotton Top Farms in St. Joseph, Missouri. The site is located in a rural area three miles outside of St. Joseph, Missouri, on a bluff which overlooks a flood plain. The area has been used for farming for over 150 years. The original farm was a dairy, 40 acres of which has been adapted into Cotton Top Farms, a small family horse farm. Cotton Top Farms was established in 1985 by the current owner functioning as a family …

A Coprological View Of Ancestral Pueblo Cannibalism, Karl Reinhard

Karl Reinhard Publications

As the object of my scientific study, I’ve chosen coprolites. It’s not a common choice, but to a paleonutritionist and archaeoparasitologist, a coprolite—a sample of ancient feces preserved by mineralization or simple drying—is a scientific bonanza. Analysis of coprolites can shed light on both the nutrition of and parasites found in prehistoric cultures. Dietary reconstructions from the analysis of coprolites can inform us about, for example, the origins of modern Native American diabetes. With regard to parasitology; coprolites hold information about the ancient emergence and spread of human infectious disease. Most sensational, however, is the recent role of coprolite analysis …

Phylogenetic Approach To The Study Of Triatomines (Triatominae, Heteroptera) = Abordagem Filogenética Em Triatomíneos (Triatominae, Heteroptera), Ester Tartarotti, M. T. V. Azeredo-Oliveira, C. R. Ceron

Harold W. Manter Laboratory of Parasitology: Library Materials

Abstracts

Triatomines are insects belonging to the Hemiptera order, Heteroptera suborder, Reduviidae family and Triatominae subfamily. All members of this subfamily are hematophagous. Triatomines evolved from Reduviidae predators and they are probably polyphyletic in origin. The combination of anatomical, physiological and ethological factors observed in this group, as well as the plesiomorphic and apomorphic characters that differentiate the five tribes and fourteen triatomine genera reinforce the polyphiletic hypotesis. However if we consider the five groups of triatomines, the Rhodniini, Cavernicolini, Bolboderini, Linshcosteini and Alberproseniini tribes constitute monophyletic groups, while the Triatomini tribe is considered polyphyletic. The New World is the …

Phylogenetic Approach To The Study Of Triatomines (Triatominae, Heteroptera) = Abordagem Filogenética Em Triatomíneos (Triatominae, Heteroptera), Ester Tartarotti, Maria Tercília Vilela De Azeredo-Oliveira, Carlos Roberto Ceron

Harold W. Manter Laboratory of Parasitology: Library Materials

Triatomines are insects belonging to the Hemiptera order, Heteroptera suborder, Reduviidae family and Triatominae subfamily. All members of this subfamily are hematophagous. Triatomines evolved from Reduviidae predators and they are probably polyphyletic in origin. The combination of anatomical, physiological and ethological factors observed in this group, as well as the plesiomorphic and apomorphic characters that differentiate the five tribes and fourteen triatomine genera reinforce the polyphiletic hypotesis. However if we consider the five groups of triatomines, the Rhodniini, Cavernicolini, Bolboderini, Linshcosteini and Alberproseniini tribes constitute monophyletic groups, while the Triatomini tribe is considered polyphyletic. The New World is the center …

The Latent Membrane Protein 1 Of Epstein-Barr Virus (Ebv) Primes Ebv Latency Cells For Type I Interferon Production, Dongsheng Xu, Kristen Brumm, Luwen Zhang

Nebraska Center for Virology: Faculty Publications

Epstein-Barr virus (EBV) latency has been associated with a variety of human cancers. Latent membrane protein 1 (LMP-1) is one of the key viral proteins required for transformation of primary B cells in vitro and establishment of EBV latency. We have previously shown that LMP-1 induces the expression of several interferon (IFN)-stimulated genes and has antiviral effect (Zhang, J., Das, S. C., Kotalik, C., Pattnaik, A. K., and Zhang, L. (2004) J. Biol. Chem. 279, 46335–46342). In this report, a novel mechanism related to the antiviral effect of LMP-1 is identified. We show that EBV type III latency cells, in …

Most Popular Downloads -- March 2006

Institutional Repository Information

The 200 most often downloaded files from the UNL Digital Commons for March 2006, with total numbers of files downloaded, and total number of downloads for the period.

Avian Influenza: Trade Issues, Toby Moore, Nancy Morgan

Zoonotics and Wildlife Disease: Publications

In 2003, an outbreak of a highly pathogenic H5N1 strain of avian influenza (AI) in Southeast Asia, notably in Vietnam but also in Thailand, focused extensive local and international media coverage on the disease and its potential human health consequences. The media coverage has followed subsequent AI outbreaks in the People’s Republic of China, the Republic of Korea, and Japan, and westward through Russia into Eastern Europe and the Middle East. Health officials remain on alert because the virus has crossed the species barrier, causing more than 100 human deaths over the last 2 years. Other deaths, most recently in …

Ard News April 2006

Agricultural Research Division: News and Annual Reports

CONTENTS:

Comments from the Dean
Layman Awards
New or Revised Projects January and February 2006
Proposals Submitted for Federal Grants January and February 2006
Grants and Contracts Received for January and February 2006

American Society Of Parasitologists Newsletter, V. 28, No. 1, Winter [I.E. Spring] 2006, Scott Lyell Gardner

American Society of Parasitologists: Newsletter

An issue of the American Society of Parasitologists' quarterly newsletter, also called the Journal of Parasitology Newsletter.

Methamphetamine Addiction, Treatment, And Outcomes: Implications For Child Welfare Workers, Cathleen Otero, Sharon Boles, Nancy Young, Kim Dennis

Center on Children, Families, and the Law (and Related Organizations): Publications

Methamphetamine is a highly addictive stimulant associated with serious health and psychiatric conditions, including heart damage and brain damage, impaired thinking and memory problems, aggression, violence, and psychotic behavior. Methamphetamine is also associated with the transmission of infectious diseases such as HIV/AIDS and hepatitis.

Child welfare workers are seeing growing numbers of children and families affected by the parent’s use of methamphetamine. In order to make sound decisions for the benefit of children and families, child welfare workers need accurate information about methamphetamine, its effects on parents and their children, and the effectiveness of treatment. This paper presents the most …

Systematics And Emerging Infectious Diseases: From Management To Solution, Daniel R. Brooks, Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The crisis of emerging infectious disease stems from the absence of comprehensive taxonomic inventories of the world's parasites, which includes the world's pathogens. Recent technological developments raise hopes that the global inventory of species, including potential pathogens, can be accomplished in a timely and cost-effective manner. The phylogenetics revolution initiated by systematists provides a means by which information about pathogen transmission dynamics can be placed in a predictive framework. Increasingly, that information is widely available in digital form on the internet. Systematic biology is well positioned to play a crucial role in efforts to be proactive in the arena of …

Bionomics Of Larvae Of Parelaphostrongylus Odocoilei (Nematoda: Protostrongylidae) In Experimentally Infected Gastropod Intermediate Hosts, Emily J. Jenkins, S. J. Kutz, Eric P. Hoberg, L. Polley

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Parelaphostrongylus odocoilei is a protostrongylid parasite that has recently been recognized at several locations in sub-Arctic, but not Arctic, North America. We investigated factors that may determine the distribution of P. odocoilei, including suitable gastropod intermediate hosts, temperature requirements for larval development in gastropods, and larval emergence facilitating overwinter transmission. We collected and experimentally infected gastropods from a site in the sub- Arctic where P. odocoilei is at the northern limit of its distribution. Deroceras laeve, Catinella sp., and Euconulus cf. fulvus, but not members of the Pupillidae, were suitable intermediate hosts. We describe bionomics of larvae …

Is This Safe To Eat?

United States Department of Agriculture Wildlife Services: Staff Publications

The harvest and consumption of wildlife is as old as humankind and often has sustained human exploration into unsettled areas. Wildlife still remain a primary foodbase for many native peoples throughout the world. From shellfish to bear, humans today continue to hunt, fish, and otherwise harvest wildlife for recreation, social and cultural needs, dietary supplementation, subsistence, and other purposes that result in the consumption of game meat (Fig. 5.1).

Over time, experience has taught people what food is safe to eat and how it should be prepared. This is especially true for those who subsist upon wildlife. Fortunately, the meat …

Geographic Factors Contributing To A High Seroprevalence Of West Nile Virus-Specific Antibodies In Humans Following An Epidemic, Beth K. Schweitzer, Wayne L. Kramer, Anthony R. Sambol, Jane L. Meza, Steven H. Hinrichs, Peter C. Iwen

United States Public Health Resources

Sera of 624 blood donors were evaluated to determine seroprevalence of West Nile virus (WNV) antibodies following the 2003 WNV epidemic in Nebraska. Geographic factors contributing to differences in WNV seropositivity were evaluated. The overall prevalence of WNV in Nebraska was higher than reported previously in other U.S. locations (9.5% WNV immunoglobulin G seroprevalence rate), with the highest prevalence identified in the western part of the state (19.7%), followed by the central (13.8%) and the eastern (4.2%) parts. Regions of the state with the highest WNV-positive mosquito rates correlated with the highest human WNV seroprevalence rates. The results showed that …

Vaccines Against Sexually Transmitted Diseases, Lynette B. Corbeil, Carlos M. Campero, Jack C. Rhyan, Robert H. Bondurant

United States Department of Agriculture Wildlife Services: Staff Publications

Human sexually transmitted infections are prevalent throughout the world. Several have been associated with adverse pregnancy outcome and increased susceptibility to HIV infection, in addition to the discomfort of inflammation of the genital tract. Yet vaccines to protect against the infection at the genital mucosa are not available. Hepatitis B is an exception, but this virus becomes systemic and protection may be at the systemic level. Sexually transmitted diseases (STDs) have long been associated with reproductive failure in cattle. These infections cause considerable economic loss, which has been a stimulus to investigation. Consequently, vaccines and mechanisms of immune protection have …

Use Of Infrared Thermography To Detect Signs Of Rabies Infection In Raccoons (Procyon Lotor), Mike R. Dunbar, Kathleen A. Maccarthy B.S.

United States Department of Agriculture Wildlife Services: Staff Publications

Infrared thermography was evaluated as a technique to determine if raccoons (Procyon lotor) experimentally infected with rabies virus could be differentiated from non-infected raccoons. Following a 10-day adjustment period, raccoons (n = 6) were infected with a virulent rabies street strain raccoon variant by injection into the masseter muscle at a dose of 2 x 10⁴ tissue-culture infectious dose (TCID₅₀) in 0.2 ml (n = 4) or 10⁵ TCID₅₀ in 1 ml (n = 2). Five of the six raccoons developed prodromal signs of rabies 17 to 22 days post-inoculation …

Feral Pigs In Queensland - Distribution, Ecology And Impact

Other Publications in Wildlife Management

Domestic pigs (Sus scrofa) were introduced to Australia by early settlers. Subsequent accidental and deliberate releases resulted in the wild (feral) population establishing throughout Australia. Feral pigs damage crops, stock and property, spread weeds and transmit diseases such as Leptospirosis and Foot and Mouth. They also cause environmental damage, digging up large areas of native vegetation and spreading weeds.

Feral pigs are declared Class 2 pests under Land Protection (Pest and Stock Route Management) Act 2002. Declaration requires landholders to control declared pest on the land under their control. A local government may serve a notice …