Digital Commons Network^™

Transcriptome Signature Of Virulent And Attenuated Pseudorabies Virus-Infected Rodent Brain, Christina Paulus, Patricia J. Sollars, Gary E. Pickard, Lynn W. Enquist

School of Veterinary and Biomedical Sciences: Faculty Publications

Mammalian alphaherpesviruses normally establish latent infections in ganglia of the peripheral nervous system in their natural hosts. Occasionally, however, these viruses spread to the central nervous system (CNS), where they cause damaging, often fatal, infections. Attenuated alphaherpesvirus derivatives have been used extensively as neuronal circuit tracers in a variety of animal models. Their circuit-specific spread provides a unique paradigm to study the local and global CNS response to infection. Thus, we systematically analyzed the host gene expression profile after acute pseudorabies virus (PRV) infection of the CNS using Affymetrix GeneChip technology. Rats were injected intraocularly with one of three selected …

Selachohemecus Benzi N. Sp. (Digenea: Sanguinicolidae) From The Blacktip Shark Carcharhinus Limbatus (Carcharhinidae) In The Northern Gulf Of Mexico, Stephen A. Bullard, Robin M. Overstreet, John K. Carlson

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Selachohemecus benzi Bullard & Overstreet n. sp. infects the heart and kidney of the blacktip shark Carcharhinus limbatus in the northern Gulf of Mexico off Florida and Mississippi, USA. Specimens of S. olsoni Short, 1954, the only congener and only other named blood fluke reported from a chondrichthyan in the Gulf of Mexico, were collected from the heart of the Atlantic sharpnose shark Rhizoprionodon terraenovae from two new localities, Apalachicola Bay, Florida, and Mississippi Sound, Mississippi, USA. The new species differs from S. olsoni by having a larger body (1.4–3.8 mm long), robust tegumental body spines numbering 51–63 along each …

Prevalence And Serovars Of Salmonella In The Feces Of Free-Ranging White-Tailed Deer (Odocoileus Virginianus) In Nebraska, David G. Renter, David P. Gnad, Jan M. Sargeant, Scott E. Hygnstrom

School of Natural Resources: Faculty Publications

To determine the prevalence and serovars of Salmonella in free-ranging deer, we cultured feces from white-tailed deer (Odocoileus virginianus) harvested by hunters during a regular firearm season in southeastern Nebraska (USA). We recovered Salmonella from 5 (1%; 95% confidence interval: 0.37– 2.20%) of 500 samples and identified four different Salmonella enterica serovars [Litchfield (1), Dessau (1), Infantis (2), and Enteritidis (1)]. Although the prevalence of Salmonella in free-ranging deer appears to be low, the serovars recovered are known to be pathogenic to humans and animals.

The Cattle Industry's Guidelines For The Care And Handling Of Cattle

Nebraska Beef Quality Assurance Program

Cattlemen have long recognized the need to properly care for livestock. Sound animal husbandry practices, based on decades of practical experience and research, are known to impact the wellbeing of cattle, individual animal health and herd productivity. Cattle are produced in very diverse environments and geographic locations in the United States. There is not one specific set of production practices that can be recommended for all cattle producers. Personal experience, training and professional judgment can serve as a valuable resource for providing proper animal care.

Oversight Of Dual-Use Biological Research: The National Science Advisory Board For Biosecurity, Dana A. Shea

Congressional Research Service Reports

Policymakers have addressed the threat of biological weapons and biosecurity issues for many years. An issue garnering increased attention is the potential for life sciences research intended to enhance scientific understanding and public health to generate results that could be misused to advance biological weapon effectiveness. Such research has been called “dual-use” research because of its applicability to both biological countermeasures and biological weapons.

The federal government is a major source of life sciences research funding. Tension over the need to maintain homeland security and support scientific endeavor has led to renewed consideration of federal policies of scientific oversight. Balancing …

“Major Sponsored Program And Faculty Awards For Research And Creative Activity For 2005

Office of Research and Economic Development: Publications

I am pleased to present the fourth annual “Major Sponsored Program and Faculty Awards for Research and Creative Activity” report. This booklet highlights the successes of University of Nebraska–Lincoln faculty during 2005. The funding sources, projects and investigators on major sponsored program awards received during the year are listed, as well as patents issued, published books and scholarship, fellowships and other recognitions. New this year are intellectual property licenses and performances and exhibitions in the fine and performing arts. The list is impressive and continues to grow each year—further evidence the University of Nebraska–Lincoln is on the move! We work …

Distributions Of Exotic Plants In Eastern Asia And North America, Qinfeng Guo, Hong Qian, Robert E. Ricklefs, Weimin Xi

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Although some plant traits have been linked to invasion success, the possible effects of regional factors, such as diversity, habitat suitability, and human activity are not well understood. Each of these mechanisms predicts a different pattern of distribution at the regional scale. Thus, where climate and soils are similar, predictions based on regional hypotheses for invasion success can be tested by comparisons of distributions in the source and receiving regions. Here, we analyse the native and alien geographic ranges of all 1567 plant species that have been introduced between eastern Asia and North America or have been introduced to both …

Primaquine: Report From Cdc Expert Meeting On Malaria Chemoprophylaxis I, David R. Hill, J. Kevin Baird, Monica E. Parise, Linda S. Lewis, Edward T. Ryan, Alan J. Magill

United States Navy: Publications

Primaquine phosphate has been used for preventing relapse of Plasmodium vivax and P. ovale malaria since the early 1950s, based on its ability to kill latent (hypnozoite) and developing liver stages of these parasites. There are three uses for primaquine in malaria: radical cure of established infection with P. vivax or P. ovale malaria; presumptive anti-relapse therapy (PART; terminal prophylaxis) in persons with extensive exposure to these parasites; and primary prophylaxis against all malaria species. All persons for whom primaquine is being considered must have a glucose-6-phosphate dehydrogenase (G6PD) enzyme level checked before use, and persons who have a deficiency …

Early Days: Genomics And Human Responses To Infection, Minghsun Liu, Stephen J. Popper, Kathleen H. Rubins, David A. Relman

United States Department of Veterans Affairs: Publications

DNA microarray-based gene transcript-profiling of the responses of primates to infection has begun to yield new insights into host–pathogen interactions; this approach, however, remains plagued by challenges and complexities that have yet to be adequately addressed. The rapidly changing nature over time of acute infectious diseases in a host, and the genetic diversity of microbial pathogens present unique problems for the design and interpretation of functional genomic studies in this field. In addition, there are the more common problems related to heterogeneity within clinical samples, the complex, non-standardized confounding variables associated with human subjects and the complexities posed by the …

Gunnison Sage-Grouse Listing Determination Literature Cited (By Category)

United States Fish and Wildlife Service: Publications

Peer Reviewed Original Research Based on Data

Peer Reviewed Secondary Research Derived

Grey Research Based on Data

Grey Literature Based on Literature Analysis

Other

Unpublished Literature

Personal Communications

Bats: Important Reservoir Hosts Of Emerging Viruses, Charles H. Calisher, James E. Childs, Hume E. Field, Tony Schountz, Kathryn V. Holmes

Zoonotics and Wildlife Disease: Publications

The remarkable mammals known as “bats” and “flying foxes” (order Chiroptera [“hand wing”]) may be the most abundant, diverse, and geographically dispersed vertebrates (Table 1). Although a great deal is known about them, detailed information is needed to explain the astonishing variations of their anatomy, their lifestyles, their roles in ecosystems ecology, and their importance as reservoir hosts of viruses of proven or potential significance for human and veterinary health.

PrpSc Accumulation In Fetal Cotyledons Of Scrapieresistant Lambs Is Influenced By Fetus Location In The Uterus, Janet Alverson, Katherine I. O'Rourke, Timothy V. Baszler

Zoonotics and Wildlife Disease: Publications

Placentae from scrapie-infected ewes have been shown to accumulate PrP^Sc when the genotype of the fetus is of a susceptible genotype (VRQ/VRQ, ARQ/VRQ or ARQ/ARQ). Cotyledons from fetuses of genotypes ARR/ARR, ARQ/ARR and ARQ/VRR have previously been shown to be resistant to PrP^Sc accumulation. By using ewes from a naturally infected scrapie flock, cotyledons from fetuses of multiple births of different genotypes were examined. PrP^Sc was detected in fetal cotyledons of genotype ARQ/ARQ, but not in cotyledons from their dizygotic twin of genotype ARQ/ARR. This confirms earlier reports of single fetuses of these genotypes, but is the …

Comparison Of Two Automated Immunohistochemical Procedures For The Diagnosis Of Scrapie In Domestic Sheep And Chronic Wasting Disease In North American White-Tailed Deer (Odocoileus Virginianus) And Mule Deer (Odocoileus Hemionus), T. V. Baszler, M. Kiupel, E. S. Williams, B. V. Thomsen, T. Gidlewski, D. L. Montgomery, Katherine I. O'Rourke, S. M. Hall

Zoonotics and Wildlife Disease: Publications

Two commercially available automated immunohistochemistry platforms, Ventana NexES and DakoCytomation Autostainer Universal Staining System, were compared for diagnosing sheep scrapie and cervid chronic wasting disease. Both automated platforms used the same antiprion protein monoclonal primary antibodies, but different platform-specific linker and amplification reagents and procedures. Duplicate sections of brainstem (at the level of the obex) and lymphoid tissue (retropharyngeal lymph node or tonsil) from the same tissue block were immunostained for the comparison. Examination of 1,020 tissues from 796 sheep revealed 100% concordance of results between the Ventana NexES and DakoCytomation platforms for diagnosing sheep scrapie from lymphoid tissue (103/103 …

Preliminary Observations Of Genetic Susceptibility Of Elk (Cervus Elaphus Nelsoni) To Chronic Wasting Disease By Experimental Oral Inoculation, Amir N. Hamir, Thomas Gidlewski, Terry R. Spraker, Janice M. Miller, Lynn Creekmore, Michelle Crocheck, Thomas Cline, Katherine I. O'Rourke

Zoonotics and Wildlife Disease: Publications

To compare the genetic susceptibility of elk (Cervus elaphus nelsoni) with various alleles of the PRNP gene, which encodes the normal cellular prion protein, to chronic wasting disease (CWD), eight 8-monthold elk calves of 3 genotypes (2 132MM, 2 132LM, and 4 132LL) were orally dosed with CWD-infected brain material from elk. During postinoculation (PI) month 23, both 132MM elk had lost appetite, developed clinical signs of weight loss and central nervous system (CNS) dysfunction, and were euthanized. Two other elk (both 132LM) developed similar clinical signs of disease and were euthanized during PI month 40. All 4 …

Nebraska Behavioral Health Situation Manual – Avian Influenza, Keith F.Hansen, Denise Bulling

University of Nebraska Public Policy Center: Publications

Purpose This exercise gives participants an opportunity to evaluate current response concepts, plans, and capabilities for a behavioral health response to an Avian Influenza outbreak. The exercise will focus on key state and local coordination, critical decisions, risk communications and the integration of external assets necessary to minimize the psychological effects of an Avian Influenza outbreak.

Scope This exercise examines the role of assets from Behavioral Health, Public Health, Agricultural, and other entities in Nebraska. Processes and decision making are more important than minute details.

Design Objectives Exercise design objectives are focused on improving understanding of a response concept, identifying …

2006 Trafficking In Persons Report, U.S. Department Of State

Human Trafficking: Data and Documents

The Department of State is required by law to submit a Report each year to the U.S. Congress on foreign governments’ efforts to eliminate severe forms of trafficking in persons. This Report is the sixth annual TIP Report. It is intended to raise global awareness, to highlight the growing efforts of the international community to combat human trafficking, and to encourage foreign governments to take effective actions to counter all forms of trafficking in persons. The Report has increasingly focused the efforts of a growing community of nations on sharing information and partnering in new and important ways.

A country …

Avian Influenza: Human Pandemic Concerns, Donna K. Carver, Elizabeth A. Krushinskie

Zoonotics and Wildlife Disease: Publications

The likelihood that the next human influenza pandemic virus will emerge from the Asian strain of the H5N1 high pathogenic bird influenza virus that is causing widespread outbreaks in Eurasia remains unknown. (See Glossary for italicized terms.) Because these bird influenza outbreaks remain primarily an animal disease, there is hope that a human pandemic can be prevented. Eradication of the H5N1 high pathogenic bird influenza virus needs to occur at the farm level in the countries where it is currently circulating. Funding of prevention, surveillance, and eradication efforts in the countries where outbreaks are occurring or in at-risk countries will …

Chronic Wasting Disease Of Elk And Deer And Creutzfeldt-Jakob Disease Comparative Analysis Of The Scrapie Prion Protein, Zhiliang Xie, Katherine I. O'Rourke, Zhiqian Dong, Allen L. Jenny, Julie A. Langenberg, Ermais D. Belay, Lawrence B. Schonberger, Robert B. Petersen, Wenquan Zou, Quingzhong Kong, Pierluigi Gambetti, Shu G. Chen

Zoonotics and Wildlife Disease: Publications

Chronic wasting disease (CWD), a transmissible prion disease that affects elk and deer, poses new challenges to animal and human health. Although the transmission ofCWDto humans has not been proven, it remains a possibility. If this were to occur, it is important to know whether the “acquired” human prion disease would show a phenotype including the scrapie prion protein (PrP^Sc) features that differ from those associated with human sporadic prion disease. In this study, we have compared the pathological profiles and PrP^Sc characteristics in brains of CWD-affected elk and deer with those in subjects with sporadic Creutzfeldt-Jakob …

Differential Expression Of Immune Response Genes In Steller Sea Lions (Eumetopias Jubatus): An Indicator Of Ecosystem Health?, Lizabeth Bowen, Brian Aldridge, Kimberlee Beckmen, Tom Gelatt, Lorrie Rea, Kathy Burek, Ken Pitcher, Jeffrey L. Stott

United States Department of Commerce: Staff Publications

Characterization of the polygenic and polymorphic features of the Steller sea lion major histocompatibility complex (MHC) provides an ideal window for evaluating immunologic vigor of the population and identifying emergence of new genotypes that reflect ecosystem pressures. MHC genotyping can be used to measure the potential immunologic vigor of a population. However, since ecosystem-induced changes to MHC genotype can be slow to emerge, measurement of differential expression of these genes can potentially provide real-time evidence of immunologic perturbations. MHC DRB genes were cloned and sequenced using peripheral blood mononuclear leukocytes derived from 10 Steller sea lions from southeast Alaska, Prince …

Preclinical And Clinical Progress Of Particle-Mediated Dna Vaccines For Infectious Diseases, Deborah Fuller, Peter Loudon, Connie Schmaljohn

United States Army: Publications

This review provides an overview of studies employing particle-mediated epidermal delivery (PMED) or the gene gun to administer DNA vaccines for infectious diseases in preclinical studies employing large animal models and in human clinical trials. It reviews the immunogenicity and protective eYcacy of PMED DNA vaccines in nonhuman primates and swine and studies that have directly compared the eVectiveness of PMED in these large animal models to existing licensed vaccines and intramuscular or intradermal delivery of DNA vaccines with a needle. Various clinical trials employing PMED have been completed and an overview of the immunogenicity, safety, and tolerability of this …

Human Transcriptome Corresponding To Human Oocytes And Use Of Said Genes Or The Corresponding Polypeptides To Trans-Differentiate Somatic Cells, Jose Bernardo Cibelli, Arif Murat Kocabas, Bing Lim, Hasan H. Otu

Department of Electrical and Computer Engineering: Faculty Publications

The identification of 101 genes upregulated or differentially expressed by mature human oocytes is provided herein. These genes and the corresponding gene products will facilitate a greater understanding of oogenesis, folliculogenesis, fertilization, and embryonic development. In addition these genes and the corresponding gene products can be used to effect dedifferentiation and/or transdifferentiation of desired somatic cells. The resultant dedifferentiated cells and somatic cells derived therefrom can be used in cell therapies such as in the treatment of cancer, autoimmunity, and other diseases wherein specific types of cells such as hematopoietic cells may be depleted because of the underlying disease or …

Department Of Veterinary And Biomedical Sciences: 2006 Annual Report

School of Veterinary and Biomedical Sciences: Information and History

Department of Veterinary and Biomedical Sciences Personnel . 9-16

Faculty .. 9

VBMS Researchers, Postdoctoral and Senior Research Associates . 10

VBMS Adjunct and Courtesy Faculty .... 11

Emeriti Faculty .. 11

VBMS Faculty and Staff by Function and Unit .. 9-16

VBMS Honors, Awards and Recognitions, 2006 17-19

VBMS Committee Assignments, 2006/2007 . 20-22

Faculty Profiles . 23-45

Raul G. Barletta, BS, MS, PhD .. 23

Bruce W. Brodersen, BS, DVM, MS, PhD .... 24

Michael P. Carlson, BS, MS, PhD ... 25

Subash Das, BSV c, MVSc, PhD .... 26

Alan R. Doster, DVM, MS, PhD, ACVP . …

Rapid Onset Of Transmission-Reducing Antibodies In Javanese Migrants Exposed To Malaria In Papua, Indonesia, J. Teun Bousema, Will Roeffen, Mike Van Der Kolk, Sake J. De Vlas, Marga Van De Vegte-Bolmer, Michael J. Bangs, Karina Teelen, Liliana Kurniawan, Jason D. Maguire, J. Kevin Baird, Robert W. Sauerwein

United States Public Health Resources

Transmission of Plasmodium falciparum malaria is initiated by sexual stages in the mosquito. Anti-Pfs48/45 and anti-Pfs230 sexual stage antibodies that are ingested together with parasites can reduce parasite development and subsequently malaria transmission. Acquisition of sexual stage immunity was studied in a cohort of 102 non-immune Javanese individuals migrating to hyperendemic Papua Indonesia. Seroprevalence of antibodies against Pfs48/45 and Pfs230 and functional transmission-reducing activity (TRA) were measured upon arrival and at 6, 12, and 24 months. Asexual parasitemia and gametocytemia were assessed every two weeks. The TRA and seroreactivity increased with the number of P. falciparum infections. The longitudinally sustained …

Implication Of A Retrovirus-Like Glycoprotein Peptide In The Immunopathogenesis Of Ebola And Marburg Viruses, Kavitha Yaddanapudi, Gustavo F. Palacios, Jonathan S. Towner, Ivy Chen, Carlos A. Sariol, Stuart T. Nichol, W. Ian Lipkin

United States Public Health Resources

Ebola and Marburg viruses can cause hemorrhagic fever (HF) outbreaks with high mortality in primates. Whereas Marburg (MARV), Ebola Zaire (ZEBOV), and Ebola Sudan (SEBOV) viruses are pathogenic in humans, apes, and monkeys, Ebola Reston (REBOV) is pathogenic only in monkeys (1–3). Early immunosuppression may contribute to pathogenesis by facilitating viral replication (4–6). Lymphocyte depletion, intravascular apoptosis, and cytokine dysregulation are prominent in fatal cases (7). Here we functionally characterize a 17 amino acid domain in filoviral glycoproteins that resembles an immunosuppressive motif in retroviral envelope proteins (8, 9). Activated human or rhesus peripheral blood mononuclear cells (PBMC) were exposed …

Norovirus Classification And Proposed Strain Nomenclature, Du-Ping Zheng, Tamie Ando, Rebecca L. Fankhauser, R. Suzanne Beard, Roger I. Glass, Stephan S. Monroe

United States Public Health Resources

Without a virus culture system, genetic analysis becomes the principal method to classify norovirus (NoV) strains. Currently, classification of NoV strains beneath the species level has been based on sequences from different regions of the viral genome. As a result, the phylogenetic insights of some virus were not appropriately interpreted, and no consensus has been reached to establish a uniform classification scheme. To provide a consistent and reliable scientific basis for classifying NoVs, we analyzed the amino acid sequences for the major capsid protein of 164 NoV strains by first using an alignment based on the predicted 3D structures. A …

Hla Alleles Associated With Delayed Progression To Aids Contribute Strongly To The Initial Cd8+ T Cell Response Against Hiv-1, Marcus Altfeld, Elizabeth T. Kalife, Ying Qi, Hendrik Streeck, Mathais Lichterfeld, Mary N. Johnston, Nicole Burgett, Martha E. Swartz, Amy Yang, Galit Alter, Xu G. Yu, Angela Meier, Juergen K. Rockstroh, Todd M. Allen, Heiko Jessen, Eric S. Rosenberg, Mary Carrington, Bruce D. Walker

United States Public Health Resources

Background

Very little is known about the immunodominance patterns of HIV-1-specific T cell responses during primary HIV-1 infection and the reasons for human lymphocyte antigen (HLA) modulation of disease progression.

Methods and Findings

In a cohort of 104 individuals with primary HIV-1 infection, we demonstrate that a subset of CD8⁺ T cell epitopes within HIV-1 are consistently targeted early after infection, while other epitopes subsequently targeted through the same HLA class I alleles are rarely recognized. Certain HLA alleles consistently contributed more than others to the total virus-specific CD8⁺ T cell response during primary infection, and also reduced …

Transfusion-Associated Transmission Of West Nile Virus, United States 2003 Through 2005, Susan P. Montgomery, Jennifer A. Brown, Matthew Kuehnert, Theresa L. Smith, Nicholas Crall, Robert S. Lanciotti, Alexandre Macedo De Oliveira, Thomas Boo, Anthony A. Marfin

United States Public Health Resources

BACKGROUND: National blood donation screening for West Nile virus (WNV) started in June 2003, after the documentation of WNV transfusion-associated transmission (TAT) in 2002.

STUDY DESIGN AND METHODS: Blood donations were screened with investigational nucleic acid amplification assays in minipool formats. Blood collection agencies (BCAs) reported screening results to state and local public health authorities. Donor test results and demographic information were forwarded to CDC via ArboNET, the national electronic arbovirus surveillance system. State health departments and BCAs also reported suspect WNV TATs to CDC, which investigated these reports to confirm WNV infection in blood transfusion recipients in …

Severe Acute Respiratory Syndrome (Sars): Knowledge, Attitudes, Practices And Sources Of Information Among Physicians Answering A Sars Fever Hotline Service, J.-F. Deng, B. Olowokure, S.C. Kaydos-Daniels, H.-J. Chang, R.S. Barwick, M.-L. Lee, C.-Y. Deng, S.H. Factor, C.-E. Chiang, S.A. Maloney, The Sars International Field Team

United States Public Health Resources

In June 2003, Taiwan introduced a severe acute respiratory syndrome (SARS) telephone hotline service to provide concerned callers with rapid access to information, advice and appropriate referral where necessary. This paper reports an evaluation of the knowledge, attitude, practices and sources of information relating to SARS among physicians who staffed the SARS fever hotline service. A retrospective survey was conducted using a self-administered postal questionnaire. Participants were physicians who staffed a SARS hotline during the SARS epidemic in Taipei, Taiwan from June 1 to 10, 2003. A response rate of 83% was obtained. All respondents knew the causative agent of …

Wildlife Tuberculosis In South African Conservation Areas: Implications And Challenges, A.L. Michel, R.G. Bengis, D.F. Keet, M. Hofmeyr, L.M. De Klerk, P.C. Cross, A.E. Jolles, D. Cooper, I.J. Whyte, P. Buss, J. Godfroid

Michigan Bovine Tuberculosis Bibliography and Database

Tuberculosis, caused by Mycobacterium bovis, was first diagnosed in African buffalo in South Africa’s Kruger National Park in 1990. Over the past 15 years the disease has spread northwards leaving only the most northern buffalo herds unaffected. Evidence suggests that 10 other small and large mammalian species, including large predators, are spillover hosts. Wildlife tuberculosis has also been diagnosed in several adjacent private game reserves and in the Hluhluwe-iMfolozi Park, the third largest game reserve in South Africa.

The tuberculosis epidemic has a number of implications, for which the full effect of some might only be seen in the long-term. …

Glucocorticoid Hormone Levels Increase With Group Size And Parasite Load In Cliff Swallows, Samrrah A. Raouf, Linda C. Smith, Mary Bomberger Brown, John C. Wingfield, Charles R. Brown

School of Biological Sciences: Faculty Publications

Animals often cope with adverse events by releasing glucocorticoid hormones, which in turn promote increased energy assimilation. In captive animals, crowding also leads to increased glucocorticoid activity, probably because of increased levels of social competition. We investigated how group size and ectoparasite infestations affected endogenous levels of the glucocorticoid hormone, corticosterone, in colonial cliff swallows, Petrochelidon pyrrhonota, in southwestern Nebraska, USA. Parasites were removed from some colonies by fumigating nests. Baseline levels of corticosterone in breeding adults varied significantly with whether parasites were present, colony size (measured by total number of active nests at a site), and nesting stage. …