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Articles 3331 - 3360 of 5498
Full-Text Articles in Entire DC Network
Using Baits To Deliver Pharmaceuticals To Feral Swine In Southern Texas, Tyler A. Campbell, Steven J. Lapidge, David B. Long
Using Baits To Deliver Pharmaceuticals To Feral Swine In Southern Texas, Tyler A. Campbell, Steven J. Lapidge, David B. Long
United States Department of Agriculture Wildlife Services: Staff Publications
Few studies have evaluated oral delivery systems of pharmaceuticals (e.g., vaccines, fertility control agents, and toxicants) to feral swine (Sus scrofa) in the United States. Our objective was to assess, through a field trial, the percentage of feral swine and non-target animals that remove and consume baits intended to transport pharmaceuticals to feral swine in southern Texas, USA. We hand-placed 1,178 iophenoxic acid (IA)–marked baits distributed over 1,721 ha (68 baits/km2) in April 2005 and monitored species-specific bait removal and consumption using track stations, automated camera systems, and serum IA values from captured animals. Ninety percent …
Anti-Capsular Antibodies Activate Killing Of Escherichia Coli O8:K87 By The Alternate Complement Pathway In Porcine Serum, N. M. Clark, E. M. Berberov, M. Wang, Rodney A. Moxley
Anti-Capsular Antibodies Activate Killing Of Escherichia Coli O8:K87 By The Alternate Complement Pathway In Porcine Serum, N. M. Clark, E. M. Berberov, M. Wang, Rodney A. Moxley
School of Veterinary and Biomedical Sciences: Faculty Publications
Enterotoxigenic Escherichia coli (ETEC) strains that produce K88 (F4) + fimbria are important causes of diarrhea and post-diarrheal septicemia in swine. ETEC O8:K87, a serotype represented by a number of these strains, is typically serum resistant. Strain-specific antibodies are known to activate alternative C pathway-mediated killing of other serum-resistant E. coli [Hill, A.W., Shears, A.L., Hibbitt, K.G., 1978. The requirement of specific antibody for the killing of E. coli by the alternate complement pathway in bovine serum. Immunology 34, 131–136], but their antigenic targets have not been determined. We tested the hypothesis that anti-K87 antibodies activate alternative pathway-mediated killing of …
Development Of A Gis-Based, Real-Time Internet Mapping Tool For Rabies Surveillance, Jesse D. Blanton, Arie Manangan, Jamie Manangan, Cathleen A. Hanlon, Dennis Slate, Charles E. Rupprecht
Development Of A Gis-Based, Real-Time Internet Mapping Tool For Rabies Surveillance, Jesse D. Blanton, Arie Manangan, Jamie Manangan, Cathleen A. Hanlon, Dennis Slate, Charles E. Rupprecht
Zoonotics and Wildlife Disease: Publications
Background: Oral rabies vaccination programs have been implemented to control the spread of wildlife rabies in the United States. However, current surveillance systems are inadequate for the efficient management and evaluation of these large scale vaccine baiting programs. With this in mind, a GIS-based rabies surveillance database and Internet mapping application was created. This surveillance system, RabID, provides a new resource for the rapid mapping and dissemination of data on animal rabies cases in relation to unaffected, enzootic, and baited areas where current interventions are underway. Results: RabID is a centralized database for diagnostic and demographic information collected by local, …
One Reserve, Three Primates: Applying A Holistic Approach To Understand The Interconnections Among Ring-Tailed Lemurs (Lemur Catta), Verreaux's Sifaka (Propithecus Verreauxi), And Humans (Homo Sapiens) At Beza Mahafaly Special Reserve, Madagascar, James E. Loudon, Michelle L. Sauther, Krista D. Fish, Mandala Hunter-Ishikawa, Youssouf Jacky Ibrahim
One Reserve, Three Primates: Applying A Holistic Approach To Understand The Interconnections Among Ring-Tailed Lemurs (Lemur Catta), Verreaux's Sifaka (Propithecus Verreauxi), And Humans (Homo Sapiens) At Beza Mahafaly Special Reserve, Madagascar, James E. Loudon, Michelle L. Sauther, Krista D. Fish, Mandala Hunter-Ishikawa, Youssouf Jacky Ibrahim
Ecological and Environmental Anthropology (University of Georgia)
We applied cultural anthropological, ethological, and parasitological methodologies to investigate the interplay among three primate species, ring-tailed lemurs (Lemur catta), Verreaux’s sifaka (Propithecus verreauxi), and humans (Homo sapiens) who live within the same habitat (i.e. in sympatry) around the Beza Mahafaly Special Reserve, Madagascar. Through a fusion of these methodologies we hope to provide a holistic understanding of the advantages and disadvantages of human-nonhuman primate sympatry. Interviews and questionnaires provided us with initial insights regarding the local peoples' attitudes toward sympatric strepsirrhine primates. Origin myths indicate a close association between humans, ring-tailed lemurs, and …
Human-Nonhuman Primate Interconnections And Their Relevance To Anthropology, Agustin Fuentes
Human-Nonhuman Primate Interconnections And Their Relevance To Anthropology, Agustin Fuentes
Ecological and Environmental Anthropology (University of Georgia)
The human-nonhuman primate interface is a core component in conservation and an emerging area of discourse across anthropology. There is a growing recognition of the relevance of long-term sympatry between human and nonhuman primates. Until recently these relationships received limited attention in the anthropological literature and in the primatological construction of models for the behavior and evolution of primate societies. Most socioecological investigations into primate groups and human populations do not incorporate their interactions (beyond predation or crop raiding), potential pathogen sharing, or the role of the anthropogenically impacted environment. Current relationships between humans and nonhuman primates are generally assumed …
Human And Non-Human Primate Co-Existence In The Neotropics: A Preliminary View Of Some Agricultural Practices As A Complement For Primate Conservation, Alejandro Estrada
Human And Non-Human Primate Co-Existence In The Neotropics: A Preliminary View Of Some Agricultural Practices As A Complement For Primate Conservation, Alejandro Estrada
Ecological and Environmental Anthropology (University of Georgia)
In this paper I address the general perception that agricultural activities are the principal threat to primate biodiversity in the tropics and argue that in Neotropical landscapes some agricultural practices may favor primate population persistence, and that this situation merits attention and investigation. To explore these issues, I examined three interrelated pressures upon tropical forests for the Mesoamerican and Amazon basin regions: human population growth trends, levels of poverty and human development and deforestation rates. I also present relevant results of recent surveys completed on the presence and activities of primate populations in agroecosystems in several landscapes in Mesoamerica. I …
Sub-Typing Of Prrsv Isolates By Means Of Measurement Of Cross-Neutralization Reactions, Fernando A. Osorio
Sub-Typing Of Prrsv Isolates By Means Of Measurement Of Cross-Neutralization Reactions, Fernando A. Osorio
School of Veterinary and Biomedical Sciences: Faculty Publications
The degree of similitude or closeness between two different isolates or strains of PRRSV is very important for deciding which strains should be used for immunization, by either vaccinating with commercial vaccines or attempting to stabilize a herd with planned wt PRRSV infection. As previously known for certain important viral diseases, such as in Foot and Mouth Disease, the reciprocal (cross-) neutralization titers between two strains may be of utmost importance to establish the degree of similarity or difference between these. There is currently a void of methods that would allow distinguishing or grouping strains of PRRSV in a manner …
Wmi Outdoor News Bulletin * November 2006, Volume 60, No. 10
Wmi Outdoor News Bulletin * November 2006, Volume 60, No. 10
Wildlife Management Institute Outdoor News Bulletin
Contents: • North American Bird Species Shown to be Susceptible to Avian Influenza • Longleaf pine restoration gets huge boost from Conservation Reserve Program • Controversy still stalking elk feedgrounds in Wyoming • Predator/prey workshop to be held at North American Conference • New book gets rave notice • With dove vote, Michiganders shoot science-based wildlife management in the foot • Geothermal energy issue still boiling at the Valles Caldera National Preserve • 72nd North American Wildlife and Natural Resources Conference registration • Northeast states get positively proactive on State Wildlife Actions Plans • Nominations sought for 2007 northern bobwhite …
Phylogenetic Analysis Of Buggy Creek Virus: Evidence For Multiple Clades In The Western Great Plains, United States Of America, Martin Pfeffer, Jerome E. Foster, Eric A. Edwards, Mary Bomberger Brown, Nicholas Komar, Charles R. Brown
Phylogenetic Analysis Of Buggy Creek Virus: Evidence For Multiple Clades In The Western Great Plains, United States Of America, Martin Pfeffer, Jerome E. Foster, Eric A. Edwards, Mary Bomberger Brown, Nicholas Komar, Charles R. Brown
United States Public Health Resources
We present the first detailed phylogenetic analysis of Buggy Creek virus (BCRV), a poorly known alphavirus with transmission cycles involving a cimicid swallow bug (Oeciacus vicarius) vector and cliff swallows (Petrochelidon pyrrhonota) and house sparrows (Passer domesticus) as the principal avian hosts. Nucleotide sequences of a 2,075-bp viral envelope glycoprotein-coding region, covering the entire PE2 gene, were determined for 33 BCRV isolates taken from swallow bugs at cliff swallow colonies in Nebraska and Colorado in the summer of 2001 and were compared with the corresponding region of BCRV isolates collected from Oklahoma in the …
Centre Canadian Cooperatif De La Sante De La Faune, Rapport Annuel 2005-2006 (French)
Centre Canadian Cooperatif De La Sante De La Faune, Rapport Annuel 2005-2006 (French)
Canadian Cooperative Wildlife Health Centre: Newsletters and Publications
À propos du CCCSF Nos activités Surveillance des maladies de la faune Surveillance des maladies de la faune - Points saillants en 2005 Centre technologique d'information du CCCSF Services d'information Formation Réponse aux maladies de la faune et gestion des maladies Publications et rapports Rapport financier 2005-2006 - Dépenses reliées au Programme de base Personnel et associés du Centre Canadian coopératif de la santé de la faune 2005-2006 Conseil d’administration du Centre Canadien coopératif de la santé de la fanue en 2005-06
Rapid Assessment For A New Invasive Species Threat: The Case Of The Gambian Giant Pouched Rat In Florida, Richard M. Engeman, John W. Woolard, Neil D. Perry, Gary W. Witmer, Scott Hardin, Lawrence Brashears, Henry Smith, Britta Muiznieks, Bernice Constantin
Rapid Assessment For A New Invasive Species Threat: The Case Of The Gambian Giant Pouched Rat In Florida, Richard M. Engeman, John W. Woolard, Neil D. Perry, Gary W. Witmer, Scott Hardin, Lawrence Brashears, Henry Smith, Britta Muiznieks, Bernice Constantin
United States Department of Agriculture Wildlife Services: Staff Publications
The Gambian giant pouched rat (Cricetomys gambianus) is a large rodent that has established a breeding population in the Florida Keys. Should it successfully disperse to mainland Florida, it could continue spreading through much of North America where significant negative ecological and agricultural consequences could result. We rapidly developed the information for implementing an efficient and successful eradication program before dispersal to the mainland occurs. This included development of monitoring and indexing methods and their application to define the animal’s range, the development of baits attractive to Gambian giant pouched rats, efficacy testing of toxicants, and development of …
American Society Of Parasitologists Newsletter, V. 28, No. 3, Late Fall 2006, Scott Lyell Gardner
American Society Of Parasitologists Newsletter, V. 28, No. 3, Late Fall 2006, Scott Lyell Gardner
American Society of Parasitologists: Newsletter
An issue of the American Society of Parasitologists' quarterly newsletter, also called the Journal of Parasitology Newsletter.
Wmi Outdoor News Bulletin * October 2006, Volume 60, No. 10
Wmi Outdoor News Bulletin * October 2006, Volume 60, No. 10
Wildlife Management Institute Outdoor News Bulletin
Contents: • Hunting/conservation organizations line up to urge expanded use of Coop Units • Suspected culprit identified in CWD transmission • Prairie Grouse Management Plan making progress • Great Lakes Fish and Wildlife Restoration Act is reauthorized • Worth reading: The Final Frontiersman
Southeastern Cooperative Wildlife Disease Study, Volume 22, October 2006, Number 3
Southeastern Cooperative Wildlife Disease Study, Volume 22, October 2006, Number 3
Southeastern Cooperative Wildlife Disease Study: Publications
The final rule on Chronic Wasting Disease (CWD) Herd Certification Program and Interstate Movement of Farmed or Captive Deer, Elk, and Moose was published in the Federal Register by the U.S. Department of Agriculture, Animal Plant and Health Inspection Service (APHIS) on July 21, 2006 (SCWDS BRIEFS, Vol. 22, No. 2). SCWDS researchers recently received research support from the U.S. Centers of Disease Control and Prevention (CDC) for a project entitled “Avian Influenza Viruses in the Environment: What is the Probability of Human Contact and Transmission?” We have had a large number of isolations of bluetongue virus (BTV) and epizootic …
Indiana Wildlife Disease News; Volume 1, Issue 4, October 2006
Indiana Wildlife Disease News; Volume 1, Issue 4, October 2006
Indiana Wildlife Disease News
Epizootic hemorrhagic disease (EHD) is an acute, infectious, often fatal viral disease of some wild ruminants. Epizootic Hemorrhagic Disease Confirmed in Free-ranging Deer From West-Central Indiana. Bluetongue is an insect-borne, viral disease primarily of sheep, occasionally goats and deer and, very rarely, cattle. The Indiana State Department of Health (ISDH) has had a sophisticated disease surveillance system in place since 2000 to monitor mosquitoes and birds that can potentially carry West Nile virus (WNV). Testing for chronic wasting disease (CWD) in Indiana’s deer herd will continue this fall. Chronic Wasting Disease Herd Certification Petitions and Request for Comments. For updates …
Canadian Cooperative Wildlife Health Centre, Annual Report For 2005-2006 (English)
Canadian Cooperative Wildlife Health Centre, Annual Report For 2005-2006 (English)
Canadian Cooperative Wildlife Health Centre: Newsletters and Publications
About the CCWHC What We Do Wildlife Disease Surveillance Wildlife Disease Surveillance - Highlights from 2005 CCWHC Information Technology Centre Information Services Education Wildlife Disease Response and Management Publications and Reports CCWHC Information Technology Centre Financial Report for 2005-2006 - Core Program Revenues Financial Report for 2005-2006 - Core Program Expenses Staff and Associates of the Canadian Cooperative Wildlife Health Centre 2005-06 Canadian Cooperative Wildlife Health Centre Board of Directors—2005-2006
Most Popular Downloads -- August 2006
Most Popular Downloads -- August 2006
Institutional Repository Information
This document lists the 150 (or so) most often downloaded documents from the UNL Digital Commons for the month of August 2006.
Fences And Deer-Damage Management: A Review Of Designs And Efficacy, Kurt C. Vercauteren, Michael J. Lavelle, Scott Hygnstrom
Fences And Deer-Damage Management: A Review Of Designs And Efficacy, Kurt C. Vercauteren, Michael J. Lavelle, Scott Hygnstrom
United States Department of Agriculture Wildlife Services: Staff Publications
White-tailed deer (Odocoileus virginianus) may cause more damage than any other species of wildlife. These damages include crop loss, automobile and aviation collisions, disease transmission, environmental degradation, and destruction of ornamental plantings. One practical method of controlling deer damage is the use of exclusionary fences. The relatively high cost of labor and materials required to build effective fences has limited most applications to the protection of orchards, vegetable farms, other high-value resources, and mitigation of human health and safety risks. Improvements in fence technology resulting in less expensive, yet effective fences have expanded the use of fences to manage damage …
Avian Influenza In Wild Birds: Status As Reservoirs, And Risks To Humans And Agriculture , Larry Clark, Jeffrey Hall
Avian Influenza In Wild Birds: Status As Reservoirs, And Risks To Humans And Agriculture , Larry Clark, Jeffrey Hall
United States Department of Agriculture Wildlife Services: Staff Publications
Influenza A viruses are naturally reservoired in wild bird populations, in which they generally exist as low-pathogenic subtypes. Historically, concern about avian influenza virus (AIV) in wild birds is related to its potential effects on agriculture and human health, and not to health issues of wild bird populations. Several subtypes (primarily H5 and H7) have caused severe outbreaks of disease in domestic bird populations. In some cases, genetic and spatial temporal analyses suggest that these high-pathogenic subtypes may originate in wild bird populations that transmit low-pathogenic forms to domestic birds; after passage in domestic birds, the low-pathogenic subtypes convert to …
Immediate And Longer Term Impact Of The Varicella Shortage On Children 18 And 24 Months Of Age In A Community Population, Barbara P. Yawn, Clayton Schroeder, Peter Wollan, Liliana Rocca, Rick Zimmerman, Barbara Bardenheier
Immediate And Longer Term Impact Of The Varicella Shortage On Children 18 And 24 Months Of Age In A Community Population, Barbara P. Yawn, Clayton Schroeder, Peter Wollan, Liliana Rocca, Rick Zimmerman, Barbara Bardenheier
United States Public Health Resources
Background: Little is known about the impact of the recent varicella vaccine shortage. To assess the temporal trend in varicella vaccine administration before 18 and 24 months of age in a community cohort of children prior to, during and after the recent varicella vaccine shortage. And to compare the temporal trends in varicella vaccinations to trends of an older, more widely accepted vaccine, the MMR.
Methods: Community population-based birth cohorts were identified who were eligible for the varicella vaccination before, during and after the 2001 to 2002 varicella vaccine shortage. Only children (84% of all) who remained in …
Most Popular Downloads -- July 2006
Most Popular Downloads -- July 2006
Institutional Repository Information
The 200 most-often downloaded documents from the UNL Digital Commons during July 2006. With links.
Ard News August 2006
Agricultural Research Division: News and Annual Reports
Contents:
Comments from the Dean
Hardin Distinguished Graduate Fellowship 2006-2007
Widaman Trust Distinguished Graduate Assistant Award 2006-2007
Shear-Miles Fellowship 2006-2007
John and Louise Skala Fellowship 2006-2007
Al Moseman International Studies Fund 2006-2007
New or Revised Projects May and June 2006
Proposals Submitted for Federal Grants May and June 2006
Grants and Contracts Received for May and June 2006
New Satellite Image Crop Maps Available for Nebraska
Aspidoderidae From North America, With The Description Of A New Species Of Aspidodera (Nematoda: Heterakoidea), F. Agustin Jimenez-Ruiz, Scott Lyell Gardner, Andrea S. Varela-Stokes
Aspidoderidae From North America, With The Description Of A New Species Of Aspidodera (Nematoda: Heterakoidea), F. Agustin Jimenez-Ruiz, Scott Lyell Gardner, Andrea S. Varela-Stokes
Harold W. Manter Laboratory of Parasitology: Faculty Publications
Aspidodera sogandaresi n. sp. (Heterakoidea: Aspidoderidae) from Dasypus novemcinctus Linnaeus, 1758 is herein described. This nematode occurs in armadillos from as far south as the canal zone of Panama, north through central Mexico, and into the southern United States. Previously identified as Aspidodera fasciata (Schneider, 1866), this new species has blunt projections on the lips and lateral expansions at the distal tips of the spicules, whereas A. fasciata has conspicuous digitiform projections on the lips, and a terminal round expansion at the tips of the spicules. Other species of the family present in North America include Aspidodera binansata Railliet and …
Susceptibility Of Greater Sage-Grouse To Experimental Infection With West Nile Virus, Larry Clark, Jeffrey Hall, Robert Mclean, Michael Dunbar, Kaci Klenk, Richard Bowen, Cynthia A. Smeraski
Susceptibility Of Greater Sage-Grouse To Experimental Infection With West Nile Virus, Larry Clark, Jeffrey Hall, Robert Mclean, Michael Dunbar, Kaci Klenk, Richard Bowen, Cynthia A. Smeraski
United States Department of Agriculture Wildlife Services: Staff Publications
Populations of greater sage-grouse (Centrocercus urophasianus) have declined 45– 80% in North America since 1950. Although much of this decline has been attributed to habitat loss, recent field studies have indicated that West Nile virus (WNV) has had a significant negative impact on local populations of grouse. We confirm the susceptibility of greater sage-grouse to WNV infection in laboratory experimental studies. Grouse were challenged by subcutaneous injection of WNV (103.2 plaque-forming units [PFUs]). All grouse died within 6 days of infection. The Kaplan-Meier estimate for 50% survival was 4.5 days. Mean peak viremia for nonvaccinated birds was 106.4 PFUs/ml (6100.2 …
Bovine Respiratory Disease In Feedlot Cattle: Environmental, Genetic, And Economic Factors, G. D. Snowder, L. Dale Van Vleck, L. V. Cundiff, G. L. Bennett
Bovine Respiratory Disease In Feedlot Cattle: Environmental, Genetic, And Economic Factors, G. D. Snowder, L. Dale Van Vleck, L. V. Cundiff, G. L. Bennett
Department of Animal Science: Faculty Publications
The objective of this study was to characterize genetic, environmental, and economic factors related to the incidence of bovine respiratory disease (BRD) in feedlot calves. Records from 18,112 calves representing 9 breeds (Angus, Braunvieh, Charolais, Gelbvieh, Hereford, Limousin, Pinzgauer, Red Poll, and Simmental) and 3 composite types (MARC I, MARC II, and MARC III) over a 15-yr period (1987 to 2001) were evaluated. Disease incidence was observed and recorded by station veterinary and technical staff. The incidence of BRD varied across years, with the annual observed incidence ranging from 5 to 44%. From 1987 to 1992, the annual average incidence …
Disease Risks Associated With Increasing Feral Swine Numbers And Distribution In The United States, Thomas Hutton, Thomas J. Deliberto, Sheldon Owen, Bruce Morrison
Disease Risks Associated With Increasing Feral Swine Numbers And Distribution In The United States, Thomas Hutton, Thomas J. Deliberto, Sheldon Owen, Bruce Morrison
Michigan Bovine Tuberculosis Bibliography and Database
Feral hogs or “wild boars” come from several sources and include released or escaped domestic swine and the truly wild European boar. When free-roaming in North America, all are included in the term “feral swine,” as are hybrids of the two types. Although morphologically distinct, both the feral swine and European wild swine are recognized as Sus scrofa. The physical damage caused by feral swine has been well documented and includes damage to vehicles, vineyards, tree plantings, archaeological sites, agricultural crops, turf, soils, rare plant communities, and wildlife habitat (Seward et al. 2004). In addition they compete with livestock …
Most Popular Downloads -- June 2006
Most Popular Downloads -- June 2006
Institutional Repository Information
The 100 most-downloaded documents for June 2006, from the University of Nebraska-Lincoln Digital Commons. With links.
American Society Of Parasitologists Newsletter, V. 28, No. 2, Late Spring 2006., Scott Lyell Gardner
American Society Of Parasitologists Newsletter, V. 28, No. 2, Late Spring 2006., Scott Lyell Gardner
American Society of Parasitologists: Newsletter
An issue of the American Society of Parasitologists' quarterly newsletter, also called the Journal of Parasitology Newsletter.
Scwds Briefs: Volume 22, Number 2 (July 2006 )
Scwds Briefs: Volume 22, Number 2 (July 2006 )
Southeastern Cooperative Wildlife Disease Study: Publications
SCWDS BRIEFS
July 2006
USDA Publishes Final CWD Rule
Chronic Wasting Disease (CWD) Herd Certification Program and Interstate Movement of Farmed or Captive Deer
International Avian Influenza Conferences
Highly pathogenic avian influenza (HPAI) H5N1 viruses
HPAI H5N1
Two New NIH Grants & Three New Grad Students
Trypanosoma cruzi
Chagas disease (American trypanosomiasis)
Dr. Susan Little
Borrelia lonestari
Southern Tick- Associated Rash Illness (STARI)
Amblyomma americanum, the lone star tick
Dr. Michael Yabsley
Protozoal Epidemics Among Frogs in Georgia
Rana heckscheri
Perkinsus-like protozoan
Polymerase chain reaction (PCR)
Parasite & Disease Problems in Mourning Doves
West Nile virus
Ascardia columbae
Trichomonas gallinae …
Research Note: First Record Of Ancylostoma Malayanum (Alessandrini, 1905) From Brown Bears (Ursus Arctos L.), Mitsuhiko Asakawa, Scott Lyell Gardner, Tsutomu Mano
Research Note: First Record Of Ancylostoma Malayanum (Alessandrini, 1905) From Brown Bears (Ursus Arctos L.), Mitsuhiko Asakawa, Scott Lyell Gardner, Tsutomu Mano
Harold W. Manter Laboratory of Parasitology: Faculty Publications
This is the first report of Ancylostoma malayanum (Alessandrini, 1905), subgenus Ceylancylostoma (Lichtenfels, 1980) from wild brown bears Ursus arctos L. The bears were collected by shooting in Wassamu-Cho province (44 [degrees] 80 [minutes] 59 [seconds] N, 142 [degrees] 82 [minutes] 99 [seconds] E), Hokkaido, Japan, November, 2003.