Digital Commons Network^™

Tinctorial Cartographies: Plant, Dye & Place, Anna Heywood-Jones

Textile Society of America: Symposium Proceedings

We live in a plant-dominated biosphere, and yet the relevance and meaning of vegetal life, beyond its contribution to human existence, is rarely considered. This way of thinking has led us to see nature as external to ourselves, as “other,” as that mysterious realm beyond the human sphere of being. As in visual culture, plant life possesses signifiers and coded meanings in its contextual configurations. Botanical literacy offers insight into environmental, sociocultural, and historical narratives of place, as the forests and herbaceous margins of our communities speak of complex past, a parallel history of survival and adaptation. Plants and textiles, …

Radical Access: Textiles And Museums, Michele Hardy, Joanne Schmidt

Textile Society of America: Symposium Proceedings

This presentation discusses recent initiatives at Glenbow and Nickle Galleries that endeavor to provide new, radical levels of access to textile collections as a means to build community and affect creativity. While locking textiles away in environmentally controlled rooms and minimizing handling are useful methods for preservation, they are less effective in building vibrant communities or creating future relevance form museum collections. This paper, building on Hemming’s post-colonial textile theory, as well as Onciul’s theories on decolonizing engagement, challenge the apparent dichotomy between access and preservation. It argues that preservation without radical access, without shared community meaning making, without respecting …

The Untold Story Of Inuit Printed Fabrics From Kinngait Studios, Kinngait (Cape Dorset), Nunavut, Canada, Roxane Shaughnessy, Anna Richard

Textile Society of America: Symposium Proceedings

The Textile Museum of Canada holds a collection of close to 200 printed fabrics designed by Inuit artists at Kinngait Studios in Kinngait (Cape Dorset), Nunavut, Canada in the 1950s and 1960s. The pieces are owned by the West Baffin Eskimo Co-operative (WBEC) and are on longterm loan to the Museum. Building on centuries-old Inuit graphic traditions, printmaking was introduced in Kinngait in 1957 as part of a larger initiative to encourage handicraft production for sale in the Canadian south. By the 1960s, the studio had a number of Inuit artists who contributed to the Kinngait Studios’ print program which …

The Rayed Head And Stepped Platform: A Core Symbol Of The Southern Andean Iconographic Series, Nancy B. Rosoff

Textile Society of America: Symposium Proceedings

This paper will explore various manifestations of the Rayed Head motif that is found on textiles produced by the Nasca, Sihuas, and Pucara cultures during the Early Intermediate Period (200 BCE – 600 CE), in the southern Andean region of South America. The Brooklyn Museum’s famous Nasca mangle, also known as “The Paracas Textile,” features repeating images of the Rayed Head motif on its interior cotton panel. Sihuas mantles also display distinctive manifestations of the motif in the form of a large rectangular head with highly stylized features and surrounded by radiating appendages. The late textile scholar and archaeologist Joerg …

Silk Velvets Identified As Byzantine: Were Warp-Looped Silk Pile Velvets Woven Under The Byzantine Empire?, Sumiyo Okumura

Textile Society of America: Symposium Proceedings

This paper will examine the possibility of whether warp-looped pile velvets, made of silk, were woven during the Byzantine Empire. This study is a continuation of my research for “Velvet and Patronage: the Origin and Historical Background of Ottoman and Italian Velvets.” Research has been conducted under these five themes: 1. Byzantine silk industry; 2. The terminology of velvet in Greek; 3. Velvets in Byzantine written sources; 4. Historical background: the relation between Byzantines and Latin powers, Turks and the Middle East; 5. Latin trade in the Black Sea and 6. Velvet production in Anatolia in the Byzantine period.

Warp And Weft Twining, And Tablet Weaving Around The Pacific, Tomoko Torimaru, Kathryn Rousso, Laura Filloy Nadal, Alejandro De Ávila B

Textile Society of America: Symposium Proceedings

Warp and weft twining predates loom-woven textiles in the archaeological record. Although it was displaced by other techniques to produce fabric in areas where it is recorded from early times, such as Egypt, this particular approach to building woven structures is still maintained in scattered areas around the world as part of local traditions with deep significance in ritual and festive life, as well in the heavy subsistence work of agricultural and hunting/fishing communities.

In this roundtable, we propose to describe, illustrate and compare warp and weft twined, and tablet woven textiles from Central America, Mexico, Canada, Alaska, China, Myanmar …

Triennial Reproduction Symposium: Looking Back And Moving Forward—How Reproductive Physiology Has Evolved, Deb L. Hamernik, Andrea S. Cupp, John S. Davis

Department of Animal Science: Faculty Publications

In honor of the 50th anniversary of the Society for the Study of Reproduction (SSR), a Triennial Reproduction Symposium was cosponsored by SSR and the American Society of Animal Science (ASAS). The Society for the Study of Reproduction was formed in 1967 when a group of reproductive biologists that were members of ASAS met with physician scientists and decided to organize a new scientific society. The goal of SSR was to promote the study of reproduction by fostering interdisciplinary communication among scientists, holding conferences, and publishing meritorious studies. Today after its 50th anniversary, the mission for SSR is to harness …

Fluid Administration Rate For Uncontrolled Intraabdominal Hemorrhage In Swine, Ujwal R. Yanala, Jason M. Johanning, Iraklis I. Pipinos, Robin R. High, Gustavo Larsen, William H. Velander, Mark A. Carlson

Department of Chemical and Biomolecular Engineering: Faculty Publications

Background We hypothesized that slow crystalloid resuscitation would result in less blood loss and a smaller hemoglobin decrease compared to a rapid resuscitation during uncontrolled hemorrhage.

Methods Anesthetized, splenectomized domestic swine underwent hepatic lobar hemitransection. Lactated Ringers was given at 150 or 20 mL/min IV (rapid vs. slow, respectively, N = 12 per group; limit of 100 mL/kg). Primary endpoints were blood loss and serum hemoglobin; secondary endpoints included survival, vital signs, coagulation parameters, and blood gases.

Results The slow group had a less blood loss (1.6 vs. 2.7 L, respectively) and a higher final hemoglobin concentration (6.0 …

Land Suitability Analysis For Emergency Medical Services Posts Along State Highways: A Case Study Of California, Ernest Tufuor, Laurence Rilett, Yunwoo Nam, Antonio Hurtado Beltran

Department of Civil and Environmental Engineering: Faculty Publications

The response time of Emergency Medical Services (EMS) to road accidents can be the difference between life and death. The California strategic highway safety plan highlight the need to improve the response time and recognizes that: 37% and 8% of the fatal crashes are 30 or more miles away from a trauma center in rural and urban areas respectively.

The paper seeks to: (1) demonstrate the viability of using spatial multi-criteria analysis in road safety management, and (2) provide a good scientific justification in selecting optimal counties for EMS posts. The goal is to propose areas that are close to …

National Beef Quality Audit–2016: Assessment Of Cattle Hide Characteristics, Offal Condemnations, And Carcass Traits To Determine The Quality Status Of The Market Cow And Bull Beef Industry, Mckensie K. Harris, L. Clay Eastwood, Courtney A. Boykin, Ashley N. Arnold, Kerri B. Gehring, Daniel S. Hale, Christopher R. Kerth, Davey B. Griffin, J.W. Savell, Keith E. Belk, Dale R. Woerner, Josh D. Hasty, Jennifer N. Martin, Ty E. Lawrence, Trenton J. Mcevers, Deborah L. Vanoverbeke, Gretchen G. Mafi, Morgan M. Pfeiffer, Ty B. Schmidt, Robert J. Maddock, D. Dwain Johnson, Chad C. Carr, Jason M. Scheffler, T. Dean Pringle, Alexander M. Stelzleni, Robert J. Delmore Jr.

Department of Animal Science: Faculty Publications

To continue the series that began in 1994, the National Beef Quality Audit (NBQA) – 2016 was conducted to quantify the quality status of the market cow and bull beef sector, as well as determine improvements made in the beef and dairy industry since 2007. The NBQA-2016 was conducted from March through December of 2016, and assessed hide-on carcasses (n = 5,278), chilled carcasses (n = 4,285), heads (n = 5,720), and offal items (n = 4,800) in 18 commercial processing facilities throughout the United States. Beef cattle were predominantly black-hided; 68.0% of beef cows and 67.2% of beef bulls …

Teens Implementing A Childhood Obesity Prevention Program In The Community: Feasibility And Perceptions Of A Partnership With Hsta And Icook 4-H, Rebecca L. Hagedorn, Jade A. White, Lisa Franzen-Castle, Sarah E. Colby, Kendra K. Kattelmann, Adrienne A. White, Melissa D. Olfert

Department of Nutrition and Health Sciences: Faculty Publications

High school student researchers and teen leaders from the Health Science Technology Academy (HSTA), under the supervision of HSTA teachers, led a childhood obesity prevention (COP) program (iCook 4-H). The objective was to evaluate the feasibility and perceptions of having teen leaders implement a COP program for dyads of youth (9–10 years old) and their primary adult food preparer. Behavior change and perceptions were assessed through surveys and open-ended interviews. Across eight HSTA organizations, 43 teen leaders participated in teaching the iCook 4-H program to 24 dyads. Increased frequency of culinary skills, physical activity and mealtime behavior were reported by …

Hidden Hunger: Solutions For America’S Aging Populations, Manfred Eggersdorfer, Ucheoma Akobundu, Regan L. Bailey, Julie Shlisky, Amy R. Beaudreault, Gilles Bergeron, Robert B. Blancato, Jeffrey B. Blumberg, Megan W. Bourassa, Filomena Gomes, Gordon Jensen, Mary Ann Johnson, Douglas Mackay, Keri Marshall, Simin Nikbin Meydani, Katherine L. Tucker

Department of Nutrition and Health Sciences: Faculty Publications

The global population, including the United States, is experiencing a demographic shift with the proportion of older adults (aged ≥ 65 years) growing faster than any other age group. This demographic group is at higher risk for developing nutrition-related chronic conditions such as heart disease and diabetes as well as infections such as influenza and pneumonia. As a result, an emphasis on nutrition is instrumental for disease risk reduction. Unfortunately, inadequate nutrient status or deficiency, often termed hidden hunger, disproportionately affects older adults because of systematic healthcare, environmental, and biological challenges. This report summarizes the unique nutrition challenges facing the …

An Impact Mapping Method To Generate Robust Qualitative Evaluation Of Community-Based Research Programs For Youth And Adults, Melissa D. Olfert, Rebecca L. Hagedorn, Jade A. White, Barbara A. Baker, Sarah E. Colby, Lisa Franzen-Castle, Kendra Kattelmann, Adrienne A. White

Department of Nutrition and Health Sciences: Faculty Publications

Ripple Effect Mapping (REM) is an evaluation approach that has traditionally been used in community settings to visually map the impact of programming and community interventions. This manuscript utilizes the Community Capitals Framework (CCF) to inform REM and to better highlight the changes and impact between various levels of a community, following a childhood obesity prevention intervention. The addition of in-depth qualitative analyses makes this approach particularly useful for the evaluation of interventions with a research–community partnership focus. The objective of this study was to describe a CCF-informed REM approach with detailed protocol, training, and application to the community-based, childhood …

Development Of Igrow: A Curriculum For Youth/Adult Dyads To Increase Gardening Skills, Culinary Competence, And Family Meal Time For Youths And Their Adult Caregivers, Jade A. White, Rebecca L. Hagedorn, Nicole L. Waterland, Makenzie L. Barr, Oluremi A. Famodu, Amy E. Root, Adrienne A. White, Sarah E. Colby, Lisa Franzen-Castle, Kendra Kattelmann, Melissa D. Olfert

Department of Nutrition and Health Sciences: Faculty Publications

This manuscript describes the development of a “learn by actively participating” curriculum for youth and their adult caregivers (dyad pair) to increase gardening skills, culinary competence, and family meal time. The curriculum was developed by integrating “iCook 4-H” and Junior Masters Gardener “Health and Nutrition from the Garden”, and “Essential Elements of 4-H Youth Development” curriculums with additional resources for gardening activities from the USDA’s My Plate and garden-based recipes. Expert reviewers (n = 11) provided feedback on the curriculum content, session structure, dosage, age appropriateness, and balance of the three focused areas. Seven family dyads (n = …

Effects Of Kernel Processing At Harvest Of Brown Midrib Corn Silage On Finishing Performance Of Steers, Lauren A. Ovinge, F. Henry Hilscher, Curtis J. Bittner Bittner, Bradley M. Boyd, John N. Anderson, Galen E. Erickson

Nebraska Beef Cattle Reports

A 2 × 3 factorial finishing study evaluated kernel processing in three corn silage hybrids on finishing performance of yearling steers fed 40% silage. The three hybrids included a control corn silage (CON), a brown midrib (bm3), and a brown midrib with a softer endosperm (bm3-EXP). No interactions were observed between hybrids and kernel processing (P > 0.45). Feeding both bm3 hybrids increased dry matter intake and average daily gain over CON (P < 0.01). Cattle fed bm3-EXP and bm3 had lower feed to gain than CON (P = 0.04), with no differences between the two brown midrib hybrids. Feeding silage that has undergone kernel processing decreased dry matter intake with similar average daily gain, which decreased feed to gain by 2.6% at 40% inclusion compared to non-processed silage (P = 0.10). The improvement in silage is calculated to be 6.5% (2.6/40) when kernel processing was utilized as compared to not kernel processing the corn silage hybrids.

Total Mercury Determination In Muscle And Liver Tissue Samples From Brazilian Amazon Fish Using Slurry Sampling, João Vitor De Queiroz, José Cavalcante Souza Vieira, Izabela Da Cunha Bataglioli, Alis Correia Bittarello, Camila Pereira Braga, Grasieli De Oliveira, Cilene Do Carmo Federici Padilha, Pedro De Magalhães Padilha

Department of Biochemistry: Faculty Publications

This paper presents a slurry sampling method for total mercury determination by graphite furnace atomic absorption spectrometry (GFAAS) in tissue of fish from the Amazon. The tissue samples were lyophilized and macerated, and then the slurry samples were prepared by putting 20 mg of tissue, added to a solution containing Triton X-100, Suprapur HNO₃, and zirconium nitrate directly in sampling vials of a spectrometer. Mercury standard solutions were prepared under the same conditions as the slurry samples. The slurry samples and the mercury standard solutions were sonicated for 20 s. Twenty microliters of slurry samples were injected into …

Towards The Synthetic Design Of Camelina Oil Enriched In Tailored Acetyl-Triacylglycerols With Medium-Chain Fatty Acids, Sunil Bansal, Hae Jin Kim, Gunnam Na, Megan E. Hamilton, Edgar B. Cahoon, Chaofu Lu, Timothy P. Durrett

Department of Biochemistry: Faculty Publications

The ability to manipulate expression of key biosynthetic enzymes has allowed the development of genetically modified plants that synthesise unusual lipids that are useful for biofuel and industrial applications. By taking advantage of the unique activities of enzymes from different species, tailored lipids with a targeted structure can be conceived. In this study we demonstrate the successful implementation of such an approach by metabolically engineering the oilseed crop Camelina sativa to produce 3-acetyl-1,2-diacyl-sn-glycerols (acetyl-TAGs) with medium-chain fatty acids (MCFAs). Different transgenic camelina lines that had been genetically modified to produce MCFAs through the expression of MCFA-specific thioesterases and …

Purification Of Hepatocytes And Sinusoidal Endothelial Cells From Mouse Liver Perfusion, Fatima Cabral, Colton M. Miller, Katrina M. Kudrna, Blake E. Hass, Jocelyn G. Daubendiek, Brianna M. Kellar, Edward N. Harris

Department of Biochemistry: Faculty Publications

This protocol demonstrates a method for obtaining high yield and viability for mouse hepatocytes and sinusoidal endothelial cells (SECs) suitable for culturing or for obtaining cell lysates. In this protocol, the portal vein is used as the site for catheterization, rather than the vena cava, as this limits contamination of other possible cell types in the final liver preparation. No special instrumentation is required throughout the procedure. A water bath is used as a source of heat to maintain the temperature of all the buffers and solutions. A standard peristaltic pump is used to drive the fluid, and a refrigerated …

A Tlr/Akt/Foxo3 Immune Tolerance–Like Pathway Disrupts The Repair Capacity Of Oligodendrocyte Progenitors, Taasin Srivastava, Parham Diba, Justin M. Dean, Fatima Banine, Daniel Shaver, Matthew Hagen, Xi Gong, Weiping Su, Ben Emery, Daniel L. Marks, Edward N. Harris, Bruce Baggentoss, Paul H. Weigel, Larry S. Sherman, Stephen A. Back

Department of Biochemistry: Faculty Publications

Cerebral white matter injury (WMI) persistently disrupts myelin regeneration by oligodendrocyte progenitor cells (OPCs). We identified a specific bioactive hyaluronan fragment (bHAf) that downregulates myelin gene expression and chronically blocks OPC maturation and myelination via a tolerance-like mechanism that dysregulates pro-myelination signaling via AKT. Desensitization of AKT occurs via TLR4 but not TLR2 or CD44. OPC differentiation was selectively blocked by bHAf in a maturation-dependent fashion at the late OPC (preOL) stage by a noncanonical TLR4/TRIF pathway that induced persistent activation of the FoxO3 transcription factor downstream of AKT. Activated FoxO3 selectively localized to oligodendrocyte lineage cells in white matter …

Reactive Oxygen Species In Chronic Obstructive Pulmonary Disease, Samia Boukhenouna, Mark A. Wilson, Karim Bahmed, Beata Kosmider

Department of Biochemistry: Faculty Publications

Chronic obstructive pulmonary disease (COPD) includes chronic bronchitis and emphysema. Environmental exposure, primarily cigarette smoking, can cause high oxidative stress and is the main factor of COPD development. Cigarette smoke also contributes to the imbalance of oxidant/antioxidant due to exogenous reactive oxygen species (ROS). Moreover, endogenously released ROS during the inflammatory process and mitochondrial dysfunction may contribute to this disease progression. ROS and reactive nitrogen species (RNS) can oxidize different biomolecules such as DNA, proteins, and lipids leading to epithelial cell injury and death. Various detoxifying enzymes and antioxidant defense systems can be involved in ROS removal. In this review, …

Role Of Selenof As A Gatekeeper Of Secreted Disulfide-Rich Glycoproteins, Sun Hee Yim, Robert A. Everley, Frank A. Schildberg, Sang-Goo Lee, Andrea Orsi, Zachary R. Barbati, Kutay Karatepe, Dmitry E. Fomenko, Petra A. Tsuji, Hongbo R. Luo, Steven P. Gygi, Roberto Sitia, Arlene H. Sharpe, Dolph L. Hatfield, Vadim N. Gladyshev

Department of Biochemistry: Faculty Publications

Selenof (15-kDa selenoprotein; Sep15) is an endoplasmic reticulum (ER)-resident thioredoxin-like oxidoreductase that occurs in a complex with UDPglucose: glycoprotein glucosyltransferase. We found that Selenof deficiency in mice leads to elevated levels of non-functional circulating plasma immunoglobulins and increased secretion of IgM during in vitro splenic B cell differentiation. However, Selenof knockout animals show neither enhanced bacterial killing capacity nor antigen-induced systemic IgM activity, suggesting that excess immunoglobulins are not functional. In addition, ER-to-Golgi transport of a target glycoprotein was delayed in Selenof knockout embryonic fibroblasts, and proteomic analyses revealed that Selenof deficiency is primarily associated with antigen presentation and ER-to-Golgi …

Dietary Omega-3 And Omega-6 Polyunsaturated Fatty Acids Modulate Hepatic Pathology, Saraswoti Khadge, J. Graham Sharp, Geoffrey M. Thiele, Timothy R. Mcguire, Lynell W. Klassen, Michael J. Duryee, Holly C. Britton, Jordan Beck, Paul N. Black, Concetta Dirusso, James Talmadge

Department of Biochemistry: Faculty Publications

Recent evidence has suggested that dietary polyunsaturated fatty acids (PUFAs) modulate inflammation; however, few studies have focused on the pathobiology of PUFA using isocaloric and isolipidic diets and it is unclear if the associated pathologies are due to dietary PUFA composition, lipid metabolism or obesity, as most studies compare diets fed ad libitum. Our studies used isocaloric and isolipidic liquid diets (35% of calories from fat), with differing compositions of omega (ω)-6 or long chain (Lc) ω-3 PUFA that were pair-fed and assessed hepatic pathology, inflammation and lipid metabolism. Consistent with an isocaloric, pair-fed model we observed no significant difference …

Long-Chain Omega-3 Polyunsaturated Fatty Acids Modulate Mammary Gland Composition And Inflammation, Saraswoti Khadge, Geoffrey M. Thiele, J. Graham Sharp, Timothy R. Mcguire, Lynell W. Klassen, Paul N. Black, Concetta Dirusso, James Talmadge

Department of Biochemistry: Faculty Publications

Studies in rodents have shown that dietary modifications as mammary glands (MG) develop, regulates susceptibility to mammary tumor initiation. However, the effects of dietary PUFA composition on MGs in adult life, remains poorly understood. This study investigated morphological alterations and inflammatory microenvironments in the MGs of adult mice fed isocaloric and isolipidic liquid diets with varying compositions of omega (ω)-6 and long-chain (Lc)-ω3FA that were pair-fed. Despite similar consumption levels of the diets, mice fed the ω-3 diet had significantly lower body-weight gains, and abdominal-fat and mammary fat pad (MFP) weights. Fatty acid analysis showed significantly higher levels of Lc-ω-3FAs …

Adenosine Has Two Faces: Regionally Dichotomous Adenosine Tone In A Model Of Epilepsy With Comorbid Sleep Disorders, Ted J. Warren, Timothy A. Simeone, D. David Smith, Ryan Grove, Jiri Adamec, Kaeli K. Samson, Harrison M. Roundtree, Deepak Madhavan, Kristina A. Simeone

Department of Biochemistry: Faculty Publications

Objective: Adenosine participates in maintaining the excitatory/inhibitory balance in neuronal circuits. Studies indicate that adenosine levels in the cortex and hippocampus increase and exert sleep pressure in sleep-deprived and control animals, whereas in epilepsy reduced adenosine tone promotes hyperexcitability. To date, the role of adenosine in pathological conditions that result in both seizures and sleep disorders is unknown. Here, we determined adenosine tone in sleep and seizure regulating brain regions of Kv1.1 knockout (KO) mice, a model of temporal epilepsy with comorbid sleep disorders.

Methods: 1) Reverse phase-high performance liquid chromatography (RP-HPLC) was performed on brain tissue to determine levels …

Influenza D Virus M2 Protein Exhibits Ion Channel Activity In Xenopus Laevis Oocytes, Evan Kesinger, Jianing Liu, Aaron Jensen, Catherine P. Chia, Andrew Demers, Hideaki Moriyama

School of Biological Sciences: Faculty Publications

A new type of influenza virus, known as type D, has recently been identified in cattle and pigs. Influenza D virus infection in cattle is typically asymptomatic; however, its infection in swine can result in clinical disease. Swine can also be infected with all other types of influenza viruses, namely A, B, and C. Consequently, swine can serve as a "mixing vessel" for highly pathogenic influenza viruses, including those with zoonotic potential. Currently, the only antiviral drug available targets influenza M2 protein ion channel is not completely effective. Thus, it is necessary to develop an M2 ion channel blocker capable …

Energy Demand And The Context-Dependent Effects Of Genetic Interactions Underlying Metabolism, Luke A. Hoekstra, Cole R. Julick, Katelyn M. Mika, Kristi L. Montooth

School of Biological Sciences: Faculty Publications

Genetic effects are often context dependent, with the same genotype differentially affecting phenotypes across environments, life stages, and sexes.We used an environmental manipulation designed to increase energy demand during development to investigate energy demand as a general physiological explanation for context-dependent effects of mutations, particularly for those mutations that affect metabolism. We found that increasing the photoperiod during which Drosophila larvae are active during development phenocopies a temperature-dependent developmental delay in a mitochondrial-nuclear genotype with disrupted metabolism. This result indicates that the context-dependent fitness effects of this genotype are not specific to the effects of temperature and may generally result …

Evolutionary Origins Of Vocal Mimicry In Songbirds, Maria Goller, Daizaburo Shizuka

School of Biological Sciences: Faculty Publications

Vocal learning is an important behavior in oscines (songbirds). Some songbird species learn heterospecific sounds as well as conspecific vocalizations. The emergence of vocal mimicry is necessarily tied to the evolution of vocal learning, as mimicry requires the ability to acquire sounds through learning. As such, tracking the evolutionary origins of vocal mimicry may provide insights into the causes of variation in song learning programs among songbirds. We compiled a database of known vocal mimics that comprised 339 species from 43 families. We then traced the evolutionary history of vocal mimicry across the avian phylogeny using ancestral trait reconstruction on …

Cd32 Is Expressed On Cells With Transcriptionally Active Hiv But Does Not Enrich For Hiv Dna In Resting T Cells, Mohamed Abdel-Mohsen, Leticia Kuri-Cervantes, Judith Grau-Exposito, Adam M. Spivak, Racheal A. Nell, Costin Tomescu, Surya Kumari Vadrevu, Leila B. Giron, Carla Serra-Peinado, Meritxell Genesca, Josep Castellvi, Guoxin Wu, Perla M. Del Rio Estrada, Mauricio Gonzalez-Navarro, Kenneth Lynn, Collin T. King, Sai Vemula, Kara Cox, Yanmin Wan, Qingsheng Li, Karam Mounzer, Jay Kostman, Ian Frank, Mirko Paiardini, Daria Hazuda, Gustavo Reyes-Teran, Douglas Richman, Bonnie Howell, Pablo Tebas, Javier Martinez-Picado, Vicente Planelles, Maria J. Buzon, Michael R. Betts, Luis J. Montaner

School of Biological Sciences: Faculty Publications

The persistence of HIV reservoirs, including latently infected, resting CD4⁺ T cells, is the major obstacle to cure HIV infection. CD32a expression was recently reported to mark CD4⁺ T cells harboring a replication-competent HIV reservoir during antiretroviral therapy (ART) suppression. We aimed to determine whether CD32 expression marks HIV latently or transcriptionally active infected CD4⁺ T cells. Using peripheral blood and lymphoid tissue of ART-treated HIV⁺ or SIV⁺ subjects, we found that most of the circulating memory CD32⁺ CD4⁺ T cells expressed markers of activation, including CD69, HLA-DR, CD25, CD38, and Ki67, and …

The Open Source Gaitor Suite For Rodent Gait Analysis, Brittany Y. Jacobs, Emily H. Lakes, Alex J. Reiter, Spencer P. Lake, Trevor R. Ham, Nic D. Leipzig, Stacy L. Porvasnik, Christine E. Schmidt, Rebecca A. Wachs, Kyle D. Allen

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Locomotive changes are often associated with disease or injury, and these changes can be quantified through gait analysis. Gait analysis has been applied to preclinical studies, providing quantitative behavioural assessment with a reasonable clinical analogue. However, available gait analysis technology for small animals is somewhat limited. Furthermore, technological and analytical challenges can limit the effectiveness of preclinical gait analysis. The Gait Analysis Instrumentation and Technology Optimized for Rodents (GAITOR) Suite is designed to increase the accessibility of preclinical gait analysis to researchers, facilitating hardware and software customization for broad applications. Here, the GAITOR Suite’s utility is demonstrated in 4 models: …

Greenhouse Gas Emissions From Beef Feedlot Surface Materials As Affected By Diet, Moisture, Temperature, And Time, Bryan L. Woodbury, John E. Gilley, David B. Parker, Bobbi S. Stomer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

A laboratory study was conducted to measure the effects of diet, moisture, temperature, and time on greenhouse gas (GHG) emissions from feedlot surface materials (FSM). The FSM were collected from open-lot pens where beef cattle were fed either a dry-rolled corn (DRC) diet containing no wet distillers grains with solubles (WDGS) or a DRC diet containing 35% WDGS. The FSM were collected, air-dried or mixed with 3.0 L of water to represent dry or wet conditions, and then incubated at temperatures of 5°C, 15°C, 25°C, or 35°C. Static flux chambers were used to quantify GHG emissions over a 14-day period. …