Digital Commons Network^™

Nuclear Receptor 4a3 (Nr4a3) Regulates Murine Mast Cell Responses And Granule Content, Gianni Garcia-Faroldi, Fabio R. Melo, Dennis Bruemmer, Orla M Conneely, Gunnar Pejler, Anders Lundequist

Internal Medicine Faculty Publications

Nuclear receptor 4a3 (Nr4a3) is a transcription factor implicated in various settings such as vascular biology and inflammation. We have recently shown that mast cells dramatically upregulate Nuclear receptor 4a3 upon activation, and here we investigated the functional impact of Nuclear receptor 4a3 on mast cell responses. We show that Nuclear receptor 4a3 is involved in the regulation of cytokine/chemokine secretion in mast cells following activation via the high affinity IgE receptor. Moreover, Nuclear receptor 4a3 negatively affects the transcript and protein levels of mast cell tryptase as well as the mast cell’s responsiveness to allergen. Together, these findings identify …

Proteoglycans: Road Signs For Neurite Outgrowth, Justin A. Beller, Diane M. Snow

Spinal Cord and Brain Injury Research Center Faculty Publications

Proteoglycans in the central nervous system play integral roles as "traffic signals" for the direction of neurite outgrowth. This attribute of proteoglycans is a major factor in regeneration of the injured central nervous system. In this review, the structures of proteoglycans and the evidence suggesting their involvement in the response following spinal cord injury are presented. The review further describes the methods routinely used to determine the effect proteoglycans have on neurite outgrowth. The effects of proteoglycans on neurite outgrowth are not completely understood as there is disagreement on what component of the molecule is interacting with growing neurites and …

A Review Of Air–Ice Chemical And Physical Interactions (Aici): Liquids, Quasi-Liquids, And Solids In Snow, T. Bartels-Rausch, H. -W. Jacobi, T. F. Kahan, J. L. Thomas, E. S. Thomson, J. P. D. Abbatt, M. Ammann, J. R. Blackford, H. Bluhm, C. Boxe, F. Domine, M. M. Frey, I. Gladich, M. I. Guzmán, D. Heger, Th. Huthwelker, P. Klán, W. F. Kuhs, M. H. Kuo, S. Maus, S. G. Moussa, V. F. Mcneill, J. T. Newberg, J. B. C. Pettersson, M. Roeselová, J. R. Sodeau

Chemistry Faculty Publications

Snow in the environment acts as a host to rich chemistry and provides a matrix for physical exchange of contaminants within the ecosystem. The goal of this review is to summarise the current state of knowledge of physical processes and chemical reactivity in surface snow with relevance to polar regions. It focuses on a description of impurities in distinct compartments present in surface snow, such as snow crystals, grain boundaries, crystal surfaces, and liquid parts. It emphasises the microscopic description of the ice surface and its link with the environment. Distinct differences between the disordered air–ice interface, often termed quasi-liquid …

Reducing Cancer Disparities Through Community Engagement In Policy Development: The Role Of Cancer Councils, Michael A. Preston, Glen P. Mays, Rise' D. Jones, Sharla A. Smith, Chara N. Stewart, Ronda Henry-Tillman

Health Management and Policy Faculty Publications

Cancer is the second leading cause of death in the U.S and a source of large racial and ethnic disparities in population health. Policy development is a powerful but sometimes overlooked public health tool for reducing cancer burden and disparities. Along with other partners in the public health system, community-based organizations such as local cancer councils can play valuable roles in developing policies that are responsive to community needs and in mobilizing resources to support policy adoption and implementation. This paper examines the current and potential roles played by local cancer councils to reduce cancer burden and disparities. Responsive public …

Metrics Of The Gynecologic Oncology Literature Focused On Cited Utilization And Costs, Edward J. Pavlik, John Hoff, Dylan Woolum, Yuqing Liang, Christiaan Wijers, Melissa Schwartz, Jason Lefringhouse, Lauren Baldwin

Obstetrics and Gynecology Faculty Publications

OBJECTIVE: The newest findings on literature utilization relevant to gynecologic oncology were published by Thomson Reuters during June 2013 as determinants of journal standing. Our objective was to assess the different metrics reported for relative impact and cost for journals relevant to gynecologic oncology.

METHODS: 55 journals were evaluated for Impact Factor (IF), 5Year IF, Immediacy Index, Cited Half Life, Eigenfactor (EF) Score, Article Influence (AI) scores and subscription costs obtained from publisher information.

RESULTS: CA-A Cancer Journal for Clinicians had the highest IF (101.78) & AI (24.502). The top EF cancer-specific journals were the Journal of Clinical Oncology, Cancer …

Ether Bridge Formation In Loline Alkaloid Biosynthesis, Juan Pan, Minakshi Bhardwaj, Jerome Ralph Faulkner, Padmaja Nagabhyru, Nikki D. Charlton, Richard M. Higashi, Anne-Frances Miller, Carolyn A Young, Robert B. Grossman, Christopher L. Schardl

Toxicology and Cancer Biology Faculty Publications

Lolines are potent insecticidal agents produced by endophytic fungi of cool-season grasses. These alkaloids are composed of a pyrrolizidine ring system and an uncommon ether bridge linking carbons 2 and 7. Previous results indicated that 1-aminopyrrolizidine was a pathway intermediate. We used RNA interference to knock down expression of lolO, resulting in the accumulation of an alkaloid identified as exo-1-acetamidopyrrolizidine based on high-resolution MS and NMR. Genomes of endophytes differing in alkaloid profiles were sequenced, revealing that those with mutated lolO accumulated exo-1-acetamidopyrrolizidine but no lolines. Heterologous expression of wild-type lolO complemented a lolO mutant, resulting in …

H-Diplo Roundtable On Paul Thomas Chamberlin. The Global Offensive: The United States, The Palestine Liberation Organization And The Making Of The Post-Cold War Order, Douglas Little, Jeffrey James Byrne, Craig Daigle, William B. Quandt, Brad Simpson, Paul Chamberlin

History Faculty Publications

No abstract provided.

Regulation Of The Proapoptotic Functions Of Prostate Apoptosis Response-4 (Par-4) By Casein Kinase 2 In Prostate Cancer Cells, A De Thonel, A Hazoumé, V Kochin, K Isoniemi, G Jego, E Fourmaux, A Hammann, H Mjahed, O Filhol, O Micheau, P Rocchi, V Mezger, J E Eriksson, Vivek M. Rangnekar, C Garrido

Radiation Medicine Faculty Publications

The proapoptotic protein, prostate apoptosis response-4 (Par-4), acts as a tumor suppressor in prostate cancer cells. The serine/threonine kinase casein kinase 2 (CK2) has a well-reported role in prostate cancer resistance to apoptotic agents or anticancer drugs. However, the mechanistic understanding on how CK2 supports survival is far from complete. In this work, we demonstrate both in rat and humans that (i) Par-4 is a new substrate of the survival kinase CK2 and (ii) phosphorylation by CK2 impairs Par-4 proapoptotic functions. We also unravel different levels of CK2-dependent regulation of Par-4 between species. In rats, the phosphorylation by CK2 at …

Ivivc From Long Acting Olanzapine Microspheres, Susan D'Souza, Jabar A. Faraj, Stefano Giovagnoli, Patrick P. Deluca

Pharmaceutical Sciences Faculty Publications

In this study, four PLGA microsphere formulations of Olanzapine were characterized on the basis of their in vitro behavior at 37°C, using a dialysis based method, with the goal of obtaining an IVIVC. In vivo profiles were determined by deconvolution (Nelson-Wagner method) and using fractional AUC. The in vitro and in vivo release profiles exhibited the same rank order of drug release. Further, in vivo profiles obtained with both approaches were nearly superimposable, suggesting that fractional AUC could be used as an alternative to the Nelson-Wagner method. A comparison of drug release profiles for the four formulations revealed that the …

Superoxide Signaling In Perivascular Adipose Tissue Promotes Age-Related Artery Stiffness, Bradley S. Fleenor, Jason S. Eng, Amy L. Sindler, Bryant T. Pham, Jackson D. Kloor, Douglas R. Seals

Graduate Center for Nutritional Sciences Faculty Publications

We tested the hypothesis that superoxide signaling within aortic perivascular adipose tissue (PVAT) contributes to large elastic artery stiffening in old mice. Young (4-6 months), old (26-28 months), and old treated with 4-Hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPOL), a superoxide scavenger (1 mm in drinking water for 3 weeks), male C57BL6/N mice were studied. Compared with young, old had greater large artery stiffness assessed by aortic pulse wave velocity (aPWV, 436 ± 9 vs. 344 ± 5 cm s(-1)) and intrinsic mechanical testing (3821 ± 427 vs. 1925 ± 271 kPa) (both P < 0.05). TEMPOL treatment in old reversed both measures of arterial stiffness. Aortic PVAT superoxide production was greater in old (P < 0.05 vs. Y), which was normalized with TEMPOL. Compared with young, old controls had greater pro-inflammatory proteins in PVAT-conditioned media (P < 0.05). Young recipient mice transplanted with PVAT from old compared with young donors for 8 weeks had greater aPWV (409 ± 7 vs. 342 ± 8 cm s(-1)) and intrinsic mechanical properties (3197 ± 647 vs. 1889 ± 520 kPa) (both P < 0.05), which was abolished with TEMPOL supplementation in old donors. Tissue-cultured aortic segments from old in the presence of PVAT had greater mechanical stiffening compared with old cultured in the absence of PVAT and old with PVAT and TEMPOL (both, P < 0.05). In addition, PVAT-derived superoxide was associated with arterial wall hypertrophy and greater adventitial collagen I expression with aging that was attenuated by TEMPOL. Aging or TEMPOL treatment did not affect blood pressure. Our findings provide evidence for greater age-related superoxide production and pro-inflammatory proteins in PVAT, and directly link superoxide signaling in PVAT to large elastic artery stiffness.

Ectopic Expression Of The Phosphomimic Mutant Version Of Arabidopsis Response Regulator 1 Promotes A Constitutive Cytokinin Response Phenotype, Jasmina Kurepa, Yan Li, Sharyn E. Perry, Jan A. Smalle

Plant and Soil Sciences Faculty Publications

Background

Cytokinins control numerous plant developmental processes, including meristem formation and activity, nutrient distribution, senescence timing and responses to both the abiotic and biotic environments. Cytokinin signaling leads to the activation of type-B response regulators (RRBs), Myb-like transcription factors that are activated by the phosphorylation of a conserved aspartate residue in their response receiver domain. Consistent with this, overexpression of RRBs does not substantially alter plant development, but instead leads to cytokinin hypersensitivity.

Results

Here we present comparative analysis of plants overexpressing Arabidopsis RRB 1 (ARR1) or a phosphomimic ARR1^D94E mutant in which the conserved aspartate-94 (D94) is replaced …

Soluble Apoe/Aβ Complex: Mechanism And Therapeutic Target For Apoe4-Induced Ad Risk, Leon M. Tai, Shipra Mehra, Varsha Shete, Steven Estus, G. William Rebeck, Guojun Bu, Mary Jo Ladu

Sanders-Brown Center on Aging Faculty Publications

The APOE4 allele of apolipoprotein E (apoE) is the greatest genetic risk factor for Alzheimer's disease (AD) compared to APOE2 and APOE3. Amyloid-β (Aβ), particularly in a soluble oligomeric form (oAβ), is considered a proximal cause of neurodegeneration in AD. Emerging data indicate that levels of soluble oAβ are increased with APOE4, providing a potential mechanism of APOE4-induced AD risk. However, the pathway(s) by which apoE4 may increase oAβ levels are unclear and the subject of continued inquiry. In this editorial review, we present the hypothesis that apoE isoform-specific interactions with Aβ, namely apoE/Aβ complex, modulate Aβ levels. Specifically, we …

Institutional Response To The Changing Legal Environment Regarding Student Safety: A Multi-Campus Case Study, Joy Blanchard

Kentucky Journal of Higher Education Policy and Practice

Confusion regarding liability for student safety and whether federal regulations prohibit information sharing have become a concern on campuses. Do current policies mitigate liability yet still serve the best interest of students? Based on interviews of nearly 30 administrators at three campuses in 2008 and 2011, this case study examines the legal considerations used when responding to such concerns, particularly alcohol and mental health. Organizational capacity and culture structure are discussed; recommendations for practice are provided.

Allocating Higher-Education Stimulus Funds In New Jersey: A Multiple-Streams Case Study, Michael W. Klein

Kentucky Journal of Higher Education Policy and Practice

This case study examines the public policy process in New Jersey and how it influenced the distribution of American Recovery and Reinvestment Act (ARRA) funds for higher education. New Jersey originally proposed to appropriate ARRA funds only to state-administered financial-aid programs in the FY2010 budget. Applying Kingdon’s (2003) public policy framework, this study explains how higher education advocates provided feedback, presented a successful alternative, and secured $39.6 million for public college and universities.

Aortic Aneurysms In Loeys-Dietz Syndrome - A Tale Of Two Pathways?, Frank Davis, Debra L. Rateri, Alan Daugherty

Saha Cardiovascular Research Center Faculty Publications

Loeys-Dietz syndrome (LDS) is a connective tissue disorder that is characterized by skeletal abnormalities, craniofacial malformations, and a high predisposition for aortic aneurysm. In this issue of the JCI, Gallo et al. developed transgenic mouse strains harboring missense mutations in the genes encoding type I or II TGF-β receptors. These mice exhibited several LDS-associated phenotypes. Despite being functionally defective, the mutated receptors enhanced TGF-β signaling in vivo, inferred by detection of increased levels of phosphorylated Smad2. Aortic aneurysms in these LDS mice were ablated by treatment with the Ang II type 1 (AT1) receptor antagonist losartan. The results from this …

Prolyl-4-Hydroxylase Α Subunit 2 Promotes Breast Cancer Progression And Metastasis By Regulating Collagen Deposition, Gaofeng Xiong, Lei Deng, Jieqing Zhu, Piotr G. Rychahou, Ren Xu

Markey Cancer Center Faculty Publications

BACKGROUND: Increased collagen deposition provides physical and biochemical signals to support tumor growth and invasion during breast cancer development. Therefore, inhibition of collagen synthesis and deposition has been considered a strategy to suppress breast cancer progression. Collagen prolyl-4-hydroxylase α subunit 2 (P4HA2), an enzyme hydroxylating proline residues in -X-Pro-Gly- sequences, is a potential therapeutic target for the disorders associated with increased collagen deposition. However, expression and function of P4HA2 in breast cancer progression are not well investigated.

METHODS: Gene co-expression analysis was performed in the published microarray datasets to identify potential regulators of collagen I, III, and IV in human …

Experimental And Numerical Study Of Carbon Fiber Oxidation, Francesco Panerai, Nagi N. Mansour, Jean Lachaud, Alexandre Martin

Mechanical Engineering Faculty Publications

The oxidation at high Knudsen number of FiberForm® , the matrix material of NASA's Phenolic Impregnated Carbon Ablator, is investigated both experimentally and numerically. The experimental setup consists of a quartz tube through a clamshell heater. Mass loss and recession of carbon preform samples are measured at temperatures between 700 and 1300 K and pressures around 2000 Pa. A volume average fiber-scale oxidation model is used to model the setup and extract the effective reactivity of the material. New values for carbon fiber reactivity are suggested and discussed.

Global Kentucky (Winter 2014), University Of Kentucky International Center

Global Kentucky

No abstract provided.

From Cardiac Optical Imaging Data To Body Surface Ecg: A Three Dimensional Ventricle Model, Yihua Zhao

Theses and Dissertations--Biomedical Engineering

Understanding the mechanisms behind unexplained abnormal heart rhythms is important for diagnosis and prevention of arrhythmias. Many studies have investigated the mechanisms at organ, tissue, cellular and molecular levels. There is considerable information available from tissue level experiments that investigate local action potential properties and from optical imaging to observe activity propagation properties at an organ level. By combining those electrophysiological properties together, in the present study we developed a simulation model that can help in estimation of the resulting body surface potentials from a specific electrical activity pattern within the myocardium. Some of the potential uses of our model …

Teens Texting, Archana Srinivas, Megan White, Hatim A. Omar

Pediatrics Faculty Publications

In this chapter we summarize the current literature on texting use amongst adolescents. A brief overview will be presented on the prevalence of texting among teenagers, the use of texting for sexting and cyberbullying as well as the dangers of texting while driving. This chapter will serve as a brief overview of these topics in order to bring to light the emerging challenges that texting presents to the mental and physical well-being of adolescents in an effort to evoke further discussion regarding the need for increasing awareness and education to parents, educators, law makers and health care providers concerning these …

Adolescence And Human Sexuality, Donald E. Greydanus, Hatim A. Omar

Pediatrics Faculty Publications

Human sexuality is a complex phenomenon involving the interaction of biology, sex, core gender identity, and gender role behavior. Successful completion of normal stages of sexuality development is important for children and adolescents to allow for optimal life as an adult. Controversies arise for clinicians as they work with their pediatric patients regarding health care sexuality issues. It is important that clinicians help these patients in an unbiased and neutral manner. As adults, these children and adolescents will function in a number of sexuality roles, whether heterosexual, homosexual, or bisexual. This chapter reviews many of these complex and critical issues …

Microsphere Spray System For Wound Coverage, Nicholas J. Andersen

Theses and Dissertations--Biomedical Engineering

Spinal fusion is used to treat diseases or disorders of the spine by fusing together two or more vertebrae. Two associated risks with spinal fusion are infection and blood loss. Administration of tranexamic acid is used to prevent blood loss, and transfusions are given following blood loss. Surgical site infections are prevented with vancomycin powder spread into the surgical wound, while established infections are treated by debridement and delivery of antibiotics for 4 to 6 weeks. The present research explored an alternate method to prevent and treat blood loss or infection in spinal fusion. Poly(lactic-co-glycolic acid) (PLGA) microspheres was used …

The New Regulation Of Small Business Capital Formation: The Impact—If Any—Of The Jobs Act, Rutheford B. Campbell Jr.

Law Faculty Scholarly Articles

The Jumpstart Our Business Startups Act (JOBS Act) was—at least apparently—driven by the desire to promote job creation by facilitating small business capital formation. The legislation was premised on the correct assumptions that small businesses create jobs and that an efficient access to capital is essential for small businesses to emerge, compete, and survive in our competitive, market economy. It is certain that the JOBS Act will have an effect on businesses’ access to external capital. With regard, however, to the capital formation efforts of small businesses—businesses that may account for more than 25% of our national economy—the analysis offered …

Menarche, Stephanie Stockburger, Hatim A. Omar

Pediatrics Faculty Publications

Menarche is a female’s first menstrual period and a landmark event for females during puberty. Historically, menarche has been thought of as a time when women were “unclean.” However, cultural acceptance and scientific knowledge of menarche has greatly increased. Menarche occurs as part of the typical progression of puberty caused by rises in luteinizing hormone (LH), follicle stimulating hormone (FSH), and estrogen. The achievement of menstruation is associated with reproductive capability. Menarche that occurs earlier or later than expected should be evaluated by an experienced clinician and may signal a serious underlying problem. Currently, researchers are evaluating whether menarche is …

The Influence Of Video Games On Adolescent Brain Activity, Joann Lianekhammy

Theses and Dissertations--Family Sciences

The current study examined electrical brain activation in adolescent participants playing three different video games. Forty-five school aged children (M=14.3 years, SD=1.5) were randomly assigned to play either a violent game, non-violent game, or a non-violent game specifically designed to "train" the brain. Electroencephalography (EEG) was recorded during video game play. Results revealed an asymmetric right hemisphere activation in the alpha band for participants in violent game group, while those in the non-violent groups exhibited left hemispheric activation. Greater right activation in emotion literature denotes signs of withdrawal or avoidance from undesired stimulus. Implications of this finding as well as …

Key Roles Of Sub-Cellular Membranes And Co-Chaperone In Tombusvirus Replication, Kai Xu

Theses and Dissertations--Plant Pathology

Positive strand RNA viruses, inculding tombusviruses, are known to utilize cellular membranes to assemble their replicase complexes (VRCs). Two tombusviruses , Tomato bushy stunt virus (TBSV) and Carnation Italian ringspot virus (CIRV), replicate on different organellar membranes, peroxisomes or endoplasmic reticulum (ER) for TBSV and mitochodria outer membranes in case of CIRV. I showed that both TBSV and CIRV replicase proteins could assemble VRCs and replicate viral RNA on purified microsomes (ER) and mitochondria. Different efficiencies of assembly was shown determined by multiple domains on TBSV or CIRV replication proteins.

To study why VRC assembly could occur on an alternative …

Awareness Of Privilege And Oppression Scale-2: Construction And Initial Validation, Michael J. Mcclellan

Theses and Dissertations--Educational, School, and Counseling Psychology

The purpose of this study was to revise the Awareness of Privilege and Oppression Scale (Montross, 2003) and to improve upon the psychometric properties of the original instrument. The APOS-2 is a diversity training outcome measure that is designed to measure the social justice-related construct awareness of privilege and oppression. I retained 26 items from the original APOS (Montross, 2003) and utilized an expert focus group to generate new test items for the APOS-2. Feedback from an expert rater group was solicited and then incorporated into the APOS-2 to help reduce the number of items, improve item content, and evaluate …

A Mixed Methods Study Of Technological Influences On Communication And Media Exposure In Military Children Experiencing Parental Deployment, Rebecca Goodney

Theses and Dissertations--Educational, School, and Counseling Psychology

Military children have been a population of interest and research speculation for several decades. Despite the research base built studying this population, many questions remain regarding their specific experiences and mental health outcomes. To accommodate the nation's needs when fueling the armed forces by the all volunteer force currently comprising the service branches, many military personnel have found themselves in circumstances including multiple deployments and deployments of lengths approximately equal to one year. With family relationships now a more prominent issue for military members, the necessity of considering the effects of deployment on these family members has become especially pertinent. …

A Multivariate Analysis Of Consumers’ Beliefs, Attitudes, And Behaviors Associated With Locally Produced Food And Farmers’ Market Patronage, Sara Williamson

Theses and Dissertations--Agricultural Economics

While farmers’ market vendors rely on loyal and frequent patrons to purchase their products, it is unclear how the intrinsic differences among farmers’ market shoppers serve as indicators of potential shopping frequency at farmers’ markets. The objectives of this thesis are to identify consumers’ intrinsic values associated with characteristics of local foods, examine how these values are reflected in consumption behaviors among farmers' market shoppers, and explore the relationship between consumption activities and shopping frequency at farmers' markets. Results suggest that the differences between frequent and infrequent farmers’ market shoppers could be explained by the individual’s levels of high and …

Boycotts To Bombs: History, Causes, And Comparison Of The Palestinian Intifadas, Leah Wilson

Lewis Honors College Capstone Collection

One of the foremost geopolitical issues of the twenty first century, the conflict between Israel and Palestine has a complicated, ominous history, and limited signs of hope for resolution in the near future. Darkened by particularly bloody and significant events such as the 1948 Arab-Israel dispute, the 1967 Six Day War, and the continuously violent actions taken near the turn of the century, the conflict continues to detrimentally impact the security of the region, the socioeconomic livelihood of Palestinians, and the dwindling influence of international actors to curb recognizably illegal settlements and flagrant human rights violations. Particularly significant to the …