Digital Commons Network^™

Rna Sequence Determinants Of A Coupled Termination-Reinitiation Strategy For Translation Of Downstream Orf In Helminthosporium Victoriae Virus 190s And Other Victoriviruses (Family Totiviridae), Hua Li

Theses and Dissertations--Plant Pathology

Double-stranded RNA fungal virus Helminthosporium victoriae virus 190S (genus Victorivirus, family Totiviridae) contains two large open reading frames (ORFs) that overlap in the tetranucleotide AUGA. Translation of the downstream ORF, which encodes the RNA-dependent RNA polymerase (RdRp), was previously proposed to depend on ribosomal reinitiation following termination of the upstream ORF, which encodes the capsid protein. In this study, I provided evidence to confirm that coupled termination-reinitiation (stop-restart) is indeed used. A dual-fluorescence method was established to define the RNA sequence determinants for RdRp translation. Stop-restart depends on a 32-nt stretch of RNA sequence immediately upstream of the …

Hearts Of Pine: Songs In The Lives Of Three Korean Survivors Of The Japanese "Comfort Women" By Joshua D. Pilzer (Review), Donna Lee Kwon

Music Faculty Publications

No abstract provided.

Impact Of Climate Change On Wheat Production In Kentucky, Kathleen Russell, Chad Lee, Rebecca L. Mcculley, David Van Sanford

Plant and Soil Sciences Research Report

Summary:

• From 2002 to 2012, Kentucky winter wheat ranged in value from $52 million to $209 million.
• Climate change and variability have the potential to significantly impact this important economic enterprise within our state.
• This report summarizes the current state of knowledge of the potential of climate change to impact wheat production in Kentucky and surrounding states.

Home Vegetable Gardening In Kentucky, Richard Durham, Shubin Saha, John Strang, Mark A. Williams, Shawn Wright, Ricardo Bessin, Kenneth W. Seebold, Nicole Ward

Agriculture and Natural Resources Publications

Before You Begin

Every aspiring gardener should follow seven steps to have a successful gardening season:

1. Plan your garden on paper before you begin.
2. Select a good gardening site that is:
   a. in full sun for at least eight hours each day,
   b. relatively level,
   c. well‑drained,
   d. close to a water source,
   e. not shaded.
3. Prepare the soil properly and add fertilizer and lime according to soil test recommendations.
4. Plan only as large a garden as you can easily maintain. Beginning gardeners often overplant, and then they fail because they cannot keep up with the tasks required. Weeds and …

A Life Span Approach To The Relationship Between Cholesterol, Late Onset Alzheimer’S Disease, And Cognitive Functioning Among Older Adults, Brian Downer

Theses and Dissertations--Gerontology

There is evidence that cholesterol presents an important risk factor for Alzheimer’s disease (AD), but the direction of this relationship is modified by age. High cholesterol during midlife and low cholesterol during late life are both associated with an increased risk for AD. This dissertation research engaged a life span approach to study the relationship between cholesterol, AD and cognitive functioning among older adults. The purpose of this research was to determine if trajectories of cholesterol from midlife through late life differ according to AD status and if these trajectories are associated with cognitive functioning during old age.

This research …

Virtual Coaching In Guatemala: Can It Be Effective For Changing Targeted Teacher Behaviors?, Ruby Owiny

Theses and Dissertations--Early Childhood, Special Education, and Counselor Education

The education system in Guatemala struggles to meet the needs of students with disabilities. There are often not enough trained special educators to meet the demand. In addition, general educators are often not trained to adequately meet diverse needs in their classrooms. Thus, schools are often unable to fully meet the needs of students with disabilities. This study sought to identify a method by which teachers could be virtually trained on a teacher behavior (i.e., specific praise) that could help meet diverse student needs. A multiple probe design across participants was used to determine if virtual training could be effective …

Hazard And Blight: Methamphetamine Waste's Insidious Persistence And Why The Federal Government Should Be Guided By Kentucky's Approach To Cleanse Natural Resources, Brandon Adcock

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

No abstract provided.

Decay Estimates On Trace Norms Of Localized Functions Of Schrödinger Operators, Aaron Saxton

Theses and Dissertations--Mathematics

In 1973, Combes and Thomas discovered a general technique for showing exponential decay of eigenfunctions. The technique involved proving the exponential decay of the resolvent of the Schrödinger operator localized between two distant regions. Since then, the technique has been been applied to several types of Schrödinger operators. This dissertation will show that the Combes--Thomas method works well with trace, Hilbert--Schmidt and other trace-type norms. The first result we prove shows exponential decay on trace-type norms of a resolvent of a Schrödinger operator localized between two distant regions. We build on this result by applying the Combes--Thomas method again to …

Three Essays On Cross-Border Mergers And Acquisitions, Derrick T. Jenniges

Theses and Dissertations--Economics

This dissertation consists of three essays on cross-border mergers and acquisitions (M&As). The first essay studies horizontal and vertical investments between Organization for Economic Cooperation and Development (OECD) countries, while the second essay examines how investment patterns vary by country development. The third essay estimates the effect of merger policy reform on cross-border M&A activity in Europe.

The first essay tests how well theories of horizontal and vertical foreign direct investment (FDI) explain observed patterns of cross-border M&As in OECD countries. Horizontal investment occurs when multinational firms produce in foreign countries to serve the foreign market, whereas vertical investment occurs …

When Cheaters Prosper: A Look At Abusive Horse Industry Practices On The Horse Show Circuit, Kjirsten Sneed

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

No abstract provided.

The Effects Of Alternate-Line Shading On Visual Search In Grid-Based Graphic Designs, Michael P. Lee

Theses and Dissertations--Psychology

Objective: The goal of this research was to determine whether alternate-line shading (zebra-striping) of grid-based displays affects the strategy (i.e., “visual flow”) and efficiency of serial search. Background: Grids, matrices, and tables are commonly used to organize information. A number of design techniques and psychological principles are relevant to how viewers’ eyes can be guided through such visual works. One common technique for grids, “zebra-striping,” is intended to guide eyes through the design, or “create visual flow” by alternating shaded and unshaded rows or columns. Method: 13 participants completed a visual serial search task. The target was embedded in a …

Role Of The "Tobacco Free Teens" Mobile Application In Adolescent Smoking, Juawanna L. Schuller

DNP Projects

Adolescent tobacco use rates have plateaued. Tobacco use education is not mandatory in all states, yet schools remain a good opportunity to reach this population. School nurses should be able to identify components of effective tobacco use prevention education programs and learn to overcome barriers to implementation of these programs. The "Tobacco Free Teens" mobile application is a novel tool that may decrease adolescent tobacco use rates. A pilot study found that the Tobacco Free Teens mobile application was satisfactory to most participants but appealed to girls more than boys. Further research is needed to determine the effectiveness of this …

Is Shakespeare Still In The Holler? The Death Of A Language Myth, Jennifer Cramer

Linguistics Faculty Publications

No abstract provided.

The Role Of Exercise In Polychlorinated Biphenyl Induced Cardiovascular Disease, Margaret O'Bryan Murphy

Theses and Dissertations--Nutritional Sciences

Cardiovascular disease remains the leading cause of death in Western societies. Endothelial dysfunction is one of the initiating steps in the development of atherosclerosis. While there is a strong correlation with a person’s genetics, lifestyle factors including smoking, physical activity, and diet can significantly increase a person’s susceptibility to the development of atherosclerosis. In addition to these lifestyle factors, there is a strong body of evidence linking exposure to environmental pollutants including persistent organic pollutants such as polychlorinated biphenyls to increased cardiovascular disease and mortality. It has been well-established that exercise protects against cardiovascular disease, but whether exercise can modulate …

Molecular Mechanism Of Human Mismatch Repair Initiation, Sanghee Lee

Theses and Dissertations--Nutritional Sciences

DNA mismatch repair (MMR) is a highly conserved pathway that maintains genomic stability primarily by correcting mismatches generated during DNA replication. MMR deficiency leads to microsatellite instability (MSI), which is a hallmark of HNPCC (Hereditary Nonpolyposis Colorectal Cancer). Human mismatch repair is initiated by MutSα, a heterodimer of MSH2 and MSH6 subunits. Mismatch binding by MutSα triggers a series of downstream MMR events including interacting and communicating with other MMR proteins. The ATPase domain of MutSα is situated in the C-termini of its both subunits, and ATP binding is required for dissociation of MutSα from a mismatch. In eukaryotic cells, …

Eigenvalue Multiplicites Of The Hodge Laplacian On Coexact 2-Forms For Generic Metrics On 5-Manifolds, Megan E. Gier

Theses and Dissertations--Mathematics

In 1976, Uhlenbeck used transversality theory to show that for certain families of elliptic operators, the property of having only simple eigenvalues is generic. As one application, she proved that on a closed Riemannian manifold, the eigenvalues of the Laplace-Beltrami operator Δ_g are all simple for a residual set of C^r metrics. In 2012, Enciso and Peralta-Salas established an analogue of Uhlenbeck's theorem for differential forms, showing that on a closed 3-manifold, there exists a residual set of C^r metrics such that the nonzero eigenvalues of the Hodge Laplacian Δ_g^(k) on k-forms are all …

Boij-Söderberg Decompositions, Cellular Resolutions, And Polytopes, Stephen Sturgeon

Theses and Dissertations--Mathematics

Boij-Söderberg theory shows that the Betti table of a graded module can be written as a linear combination of pure diagrams with integer coefficients. In chapter 2 using Ferrers hypergraphs and simplicial polytopes, we provide interpretations of these coefficients for ideals with a d-linear resolution, their quotient rings, and for Gorenstein rings whose resolution has essentially at most two linear strands. We also establish a structural result on the decomposition in the case of quasi-Gorenstein modules. These results are published in the Journal of Algebra, see [25].

In chapter 3 we provide some further results about Boij-Söderberg decompositions. We …

Post-Myriad Genetics Copyright Of Synthetic Biology And Living Media, Michael D. Murray

Law Faculty Scholarly Articles

This Article addresses copyright as a viable form of intellectual property protection for living, organic creations of science and art. The United States Supreme Court's decision in Association for Molecular Pathology v. Myriad Genetics, Inc. narrowed patent-eligible protection over living components of humans or other organisms. Synthetic biologists are expected to look with renewed focus on copyright law for the intellectual property protection of biological creations. The contribution of this Article is to reveal that the same issues are raised with regard to the copyrightability of the works of synthetic biology as are raised by pictorial, graphic, and sculptural arts …

Order Matters: Typology Of Dual-Degreed Law Librarians, James M. Donovan

Law Faculty Scholarly Articles

To a great extent law librarianship has regarded the dual-degreed librarian as too familiar and uncomplicated to merit extended attention. The present discussion challenges this assumed simplicity. The nature of professional education is to work on deeper personal levels to create a particular identity and to inculcate specific values necessary to the successful practice of the vocation. Such fundamental effects are neither easily erased nor superseded by a later professional indoctrination. Understood in this way, professional education produces an outcome that defies the commutative property. Order matters. Librarians who go to law school (i.e., “libyers”) should be discernible from lawyers …

The Writing Is On The Wall: Using Padlet For Whole-Class Engagement, Beth Fuchs

Library Faculty and Staff Publications

Many of us define success in the classroom by the quality and quantity of student participation, but despite our best efforts, there are substantial barriers in place that discourage students outside of the vocal minority from getting more involved in our classes. This paper describes the use of a "graffiti wall" as found on Padlet (padlet.com) to overcome some of the challenges involved in class participation in order to encourage whole-class collaboration and engagement.

Kentucky Geological Survey Landslide Inventory: From Design To Application, Matthew M. Crawford

Information Circular--KGS

The Kentucky Geological Survey is compiling a landslide inventory database to better document the distribution and geologic context of Kentucky’s landslides. The database provides users with easy access to landslide information, raises awareness of landslide causes, and will help prevent property damage or injury. The database was used to create an online landslide information map, which provides online access to landslide data and gives users the ability to customize the map using other data layers pertinent to landslides. The database design is based on common attributes collected by other states with active inventories and landslide hazard programs, as well as …

Children, Violence And Bullying, Joav Merrick, Isack Kandel, Hatim A. Omar

Pediatrics Faculty Publications

Adolescent violence is a very visible phenomenon in our modern society, where you just have to open the newspaper or the television and you find yourself right in the middle of it. In order to understand the scope of the problem, we need to look at the epidemiology of global violence. Each year, more than 1.6 million people worldwide lose their lives to violence and for every person who dies as a result of violence, many more are injured and suffer from a range of physical, sexual, reproductive and mental health problems. Violence places a massive burden on national economies …

Adolescent Violence Prevention, Hatim A. Omar

Pediatrics Faculty Publications

Violence in adolescence has seen an increase since the 1990s with dramatic statistics on violent death and risk behaviors. School violence has been focused upon by a huge media coverage of especially violent cases that could have had some endemic consequences worldwide. We present a case of a 14 year old white male with change in school behavior, strategies for the case investigation, its results and long term prevention. Other research has shown that preventive measures during pregnancy, infancy and childhood can prevent adolescent and adult delinquency.

A Grassroots Program For Youth Suicide Prevention, Stephanie Stockburger, Hatim A. Omar

Pediatrics Faculty Publications

Suicide is a major public health problem. It affects people of all ages. Youth, in particular, have a disturbingly high suicide rate. Suicide is the third leading cause of death for young people between the ages of 10 and 24 (1). Approximately 4600 young people die each year from a suicide attempt (1). The most common methods used in suicides are firearms (45%), suffocation (which may be in the form of hanging) (40%), and poisoning (8%) (1). However, more young people survive suicide attempts than actually die from the attempt. A national survey of youth in both private and public …

Soil And Biosolid Nano- And Macro-Colloid Properties And Contaminant Transport Behavior, Jessique L. Ghezzi

Theses and Dissertations--Plant and Soil Sciences

Despite indications that they are potential contaminant transport systems and threats to groundwater quality, very little effort has been invested in comparing contaminant transport behavior of natural environmental nanocolloids and their corresponding macrocolloid fractions in the presence of As, Se, Pb, and Cu contaminants. This study involved physico-chemical, mineralogical, stability and contaminant-transport characterizations of nano- (< 100 nm) and macro-colloids (100-2000 nm) fractionated from three Kentucky soils and one biosolid waste. Particle size was investigated with SEM/TEM and dynamic light scattering. Surface reactivity was estimated using CEC and zeta potential. Mineralogical composition was determined by XRD, FTIR, and thermogravimetric analyses. Sorption isotherms assessed affinities for Cu²⁺, Pb²⁺, AsO₃^-, and SeO₄^-2 contaminants, while settling kinetics experiments of suspensions at 0, 2 and 10 mg/L contaminants determined stability and transportability potential. Undisturbed 18x30 cm KY Ashton Loam soil monoliths were also used for …

Using Inoculation Messages To Protect “Stay In The Market” Beliefs During Financial Crises, Lindsay Lyles Dillingham

Theses and Dissertations--Communication

This paper focuses on the problem of collapsed “stay in the market” (SIM) beliefs during financial crises. The primary purpose of this investigation was to ascertain whether or not inoculation messages represent a viable communication strategy to preemptively protect SIM beliefs during forthcoming financial crises. Ancillary purposes of this study were to further investigate the role of print and video crises, explicit instructions regarding post-inoculation talk (PIT), and gain and loss frame inoculation messages on the inoculation process. This study used a between subjects factorial design (3 x 2 plus four additional conditions) to explore ten hypotheses. Data collected from …

The Effect Of Mediated Immediacy Upon State Motivation And Cognitive Learning In An Online Lesson, Gary K. Hughes

Theses and Dissertations--Communication

The role of teacher immediacy and its impact upon student learning within the traditional classroom has been established within the instructional communication discipline in the past 30 years. In recent years, with the advent of computer-mediated distance education (i.e. online courses), some researchers have attempted to apply the same theories and measures of concepts without making the distinction between actual teacher behavioral indicants of immediacy and student perceptions of immediacy, nor recognizing that there may be a different number of variables involved between a single lesson presented online and an entire course presented over a period of time. Building upon …

Examining Cyberbullying Bystander Behavior Using A Multiple Goals Perspective, Sarah E. Jones

Theses and Dissertations--Communication

Cyberbullying, defined as any behavior performed through electronic or digital media by individuals or groups that repeatedly communicates hostile or aggressive messages intended to inflict harm or discomfort on others, is a widespread problem. Bystanders play an integral role in the initiation, maintenance, and prolonged presence of such aggressive behaviors, but have thus far been overlooked in cyberbullying literature. Cyberbullying bystanders are defined in this study as those who witness cyberbullying, either within or outside their personal social network(s) and whose available responses range from inaction to intervention. Operating from a social-ecological perspective and guided by multiple goals theories, this …

Clearing The Smoke: Understanding Organizational Change Communication And Misalignment In High-Risk Contexts, Laura Elizabeth Young

Theses and Dissertations--Communication

Recent economic turbulence in the United States has resulted in budget cuts for many city-funded organizations, including high-risk organizations such as local fire departments. Budget cuts trigger organizational change and create uncertainty among employees, which is a major concern for high-risk organizations. This dissertation examined internal communication practices used during organizational change in an urban fire department and the influence of organizational structure and culture on communication satisfaction. This robust case study used a multi-method approach including interviews with middle managers (i.e., district majors), and focus groups and channel preference surveys with full-time firefighters from lower level ranks (i.e., firefighters, …

The Development And Validation Of The Online Learning Climate Scale: A Three-Phase Study, Renee Monique Kaufmann

Theses and Dissertations--Communication

With the increasing popularity of and demand for online learning in higher education (Konetes, 2011) comes a need to examine students’ perceptions about classroom climate in these environments. Using the Instructional Beliefs Model (IBM) as a theoretical framework, this dissertation proposes the online learning climate (OLCS) scale for doing so. Informed by both organizational and instructional communication, as well as education, the scale consists of several variables related to instructor role(s) and behaviors, student characteristics, and course-specific structural issues to explain how students perceive climate within a computer-mediated classroom. Ultimately, this three-phase study consisted of: (a) constructing the OLCS, (b) …