Digital Commons Network^™

Novel Telomere-Anchored Pcr Approach For Studying Sexual Stage Telomeres In Aspergillus Nidulans, Nengding Wang, Saajidha Rizvydeen, Mithaq Vahedi, Daysi M. Vargas Gonzalez, Amanda L. Allred, Dustin Wayne Perry, Peter M. Mirabito, Karen E. Kirk

Biology Faculty Publications

Telomere length varies between germline and somatic cells of the same organism, leading to the hypothesis that telomeres are lengthened during meiosis. However, little is known about the meiotic telomere length in many organisms. In the filamentous fungus Aspergillus nidulans, the telomere lengths in hyphae and asexual spores are invariant. No study using existing techniques has determined the telomere length of the sexual ascospores due to the relatively low abundance of pure meiotic cells in A. nidulans and the small quantity of DNA present. To address this, we developed a simple and sensitive PCR strategy to measure the telomere length …

Activation Of C-Met And Upregulation Of Cd44 Expression Are Associated With The Metastatic Phenotype In The Colorectal Cancer Liver Metastasis Model, Victoria Allison Elliott, Piotr G. Rychahou, Yekaterina Y. Zaytseva, B. Mark Evers

Markey Cancer Center Faculty Publications

Background

Liver metastasis is the most common cause of death in patients with colorectal cancer. Despite extensive research into the biology of cancer progression, the molecular mechanisms that drive colorectal cancer metastasis are not well characterized.

Methods

HT29 LM1, HT29 LM2, HT29 LM3 cell lines were derived from the human colorectal cancer cell line HT29 following multiple rounds of in vivo selection in immunodeficient mice.

Results

CD44 expression, a transmembrane glycoprotein involved in cell-cell and cell-matrix adhesions, and cancer cells adhesion to endothelial cells was increased in all in vivo selected cell lines, with maximum CD44 expression and cancer cells …

The Writing Is On The Wall: Using Padlet For Whole-Class Engagement, Beth Fuchs

Library Presentations

Peer learning and class participation are essential components of the active learning classroom, so what can you do to promote full student engagement with your tried-and-true activities in a once-and-done information literacy session? Take them to the wall! Padlet (www.padlet.com) provides a free, multimedia-friendly wall which can be used to encourage real-time, whole-class participation and assessment. No special equipment or technical know-how required. Find out how one librarian has used Padlet to help students create memorable, collaborative artifacts of classroom learning experiences. This is student engagement as scholarly graffiti. Banksy, watch out!

PARTICIPANTS WILL:

• Be able to contribute to a …

Planning Professional Learning, Thomas R. Guskey

Educational, School, and Counseling Psychology Faculty Publications

With backward planning, schools can ensure that they choose professional development activities aligned with their most important goals.

Technology And Client Communications: Preparing Law Students And New Lawyers To Make Choices That Comply With The Ethical Duties Of Confidentiality, Competence, And Communication, Kristin J. Hazelwood

Law Faculty Scholarly Articles

That the use of technology has radically changed the legal profession is beyond dispute. Through technology, lawyers can now represent clients in faraway states and countries, and they can represent even local clients through a “virtual law office.” Gone are the times in which the lawyer’s choices for communicating with clients primarily involve preparing formal business letters to convey advice, holding in-person client meetings in the office, or conducting telephone calls with clients on landlines from the confines of the lawyer’s office. Not only do lawyers have choices about how to communicate with their clients, but they also frequently choose …

Variation In Vegetation And Microbial Linkages With Slope Aspect In A Montane Temperate Hardwood Forest, Frank S. Gilliam, Radim Hédl, Markéta Chudomelová, Rebecca L. Mcculley, Jim A. Nelson

Plant and Soil Sciences Faculty Publications

Plant ecologists have long been interested in aspect-related contrasts of montane forests. Few studies have assessed correlation (linkage) among vegetation strata; fewer have included soil microbial communities. This study assessed contrasts in overstory, spring herbaceous, and soil microbial communities between northeast (NE) - and southwest (SW) -facing slopes in a second-growth West Virginia hardwood forest. We addressed three questions: (1) how do soil microbial, herbaceous layer, and overstory communities vary with slope aspect? (2) do forest vegetation strata and soil microbial communities exhibit linkage? (3) do biotic relationships and linkage vary with slope aspect? Moisture, organic matter, pH, soil NO …

Farewell, School House Rock (Understanding Legislative History Through The Lens Of The Aca), Nicole Huberfeld

Law Faculty Popular Media

In this blog post, Professor Nicole Huberfeld reviews John Cannan's article A Legislative History of the Affordable Care Act: How Legislative Procedure Shapes Legislative History, 105 L. Library J. 131 (2013).

Opioid-Ketamine And Norketamine Codrug Combinations For Pain Management, Joseph R. Holtman, Peter A. Crooks, Ujjwal Chakraborty

Pharmaceutical Sciences Faculty Patents

The present invention relates to the field of pain management, and more particularly to synergistic codrugs comprising an opioid and ketamine or norketamine which have been combined to form a single chemical codrug entity. When the codrug is administered it produces a synergistic analgesic response to pain.

Editors' Preface And Acknowledgments, Rachael Hoy, Christina Williams

disClosure: A Journal of Social Theory

No abstract provided.

Tapestry Of Space: Domestic Architecture And Underground Communities In Margaret Morton’S Photography Of A Forgotten New York, Irina Nersessova

disClosure: A Journal of Social Theory

This article addresses the impact urban space has on individuals through the use of Situationist International theory and psychogeography. Representations of homelessness in New York in Margaret Morton's photography are used to demonstrate the interconnectedness among space, people, and social issues. Social issues manifest themselves in urban decay, and the inhabitants react to this phenomenon emotionally and artistically. Some inhabitants demonstrate their relationship with space by responding with material production of housing and art, which they accomplish by building without exploiting the environment the way the manufacturing of commodities often does.

Collective Counter Cartography From Prinzessinnengarten, Berlin, Gabriel Wulff

disClosure: A Journal of Social Theory

No abstract provided.

Noncanonical Role For The Host Vps4 Aaa+ Atpase Escrt Protein In The Formation Of Tomato Bushy Stunt Virus Replicase, Daniel Barajas, Isabel Fernández De Castro Martín, Judit Pogany, Cristina Risco, Peter D. Nagy

Plant Pathology Faculty Publications

Assembling of the membrane-bound viral replicase complexes (VRCs) consisting of viral- and host-encoded proteins is a key step during the replication of positive-stranded RNA viruses in the infected cells. Previous genome-wide screens with Tomato bushy stunt tombusvirus (TBSV) in a yeast model host have revealed the involvement of eleven cellular ESCRT (endosomal sorting complexes required for transport) proteins in viral replication. The ESCRT proteins are involved in endosomal sorting of cellular membrane proteins by forming multiprotein complexes, deforming membranes away from the cytosol and, ultimately, pinching off vesicles into the lumen of the endosomes. In this paper, we show an …

Acetobixan, An Inhibitor Of Cellulose Synthesis Identified By Microbial Bioprospecting, Ye Xia, Lei Lei, Chad Brabham, Jozsef Stork, James R. Strickland, Adam Ladak, Ying Gu, Ian Wallace, Seth Debolt

Horticulture Faculty Publications

In plants, cellulose biosynthesis is an essential process for anisotropic growth and therefore is an ideal target for inhibition. Based on the documented utility of small-molecule inhibitors to dissect complex cellular processes we identified a cellulose biosynthesis inhibitor (CBI), named acetobixan, by bio-prospecting among compounds secreted by endophytic microorganisms. Acetobixan was identified using a drug-gene interaction screen to sift through hundreds of endophytic microbial secretions for one that caused synergistic reduction in root expansion of the leaky AtcesA6prc1-1 mutant. We then mined this microbial secretion for compounds that were differentially abundant compared with Bacilli that failed to mimic CBI action …

Overview Of Ablation Test-Cases, Alexandre Martin, Jean Lachaud, Tom Van Eekelen, Ioana Cozmuta, Michael Wright, Nagi N. Mansour, Daniele Bianchi

Ablation Workshop

No abstract provided.

Surface Effects And Phenomena On Ablative Materials, Alexandre Martin

Ablation Workshop

No abstract provided.

The Public Health Pbrn Program: A Status Update, Glen P. Mays

Health Management and Policy Presentations

As of 2014 the Robert Wood Johnson Foundation's Public Health Practice-Based Research Networks Program has engaged more than 1600 U.S. public health agencies in research production and application, generating more than 60 ongoing and completed empirical studies, 70 peer-reviewed publications, and 200 presentations and professional and scientific meetings.

Creating Learning Systems: Lessons From Public Health Practice-Based Research Networks, Glen P. Mays

Health Management and Policy Presentations

Practice-based research networks (PBRNs) provide powerful mechanisms for implementing research studies that evaluate the health and economic effects of delivery system innovations. This presentation reviews strategies, emerging findings, and lessons learned from PBRNs and related studies conducted in U.S. public health settings. We give special focus to studies that examine multi-organizational and cross-sectoral strategies for population health improvement.

An Intersectional Approach To Latino Anti-Violence Engagement, M. Cristina Alcalde

Gender and Women's Studies Faculty Publications

Men’s perspectives on nonviolence is a relatively rare topic of feminist research. This article examines Latinos’ pathways to a commitment to nonviolent masculinities within intimate relationships. Examining literature on nonviolence and drawing on interviews with six Latinos the article uses a feminist ecological approach to identify and examine contributing factors to “emergent masculinities” characterized by a commitment to nonviolence in intimate relationships. It examines experiences of marginalization, exposure to domestic violence, and the influence of a close woman mentor as factors that contribute to a commitment to nonviolence. It also critiques the common equation of the absence of physical violence …

Big Data, Social Physics, And Spatial Analysis: The Early Years, Trevor J. Barnes, Matthew W. Wilson

Geography Faculty Publications

This paper examines one of the historical antecedents of Big Data, the social physics movement. Its origins are in the scientific revolution of the 17th century in Western Europe. But it is not named as such until the middle of the 19th century, and not formally institutionalized until another hundred years later when it is associated with work by George Zipf and John Stewart. Social physics is marked by the belief that large-scale statistical measurement of social variables reveals underlying relational patterns that can be explained by theories and laws found in natural science, and physics in particular. This larger …

A Marriage Of Convenience: The Role Of The Russian Orthodox Church In Contemporary Russian Politics, S. Elliot Estebo

Ex-Patt Magazine

Since 2000, the roles of the Russian Orthodox Church (ROC) in society and its ties to the Russian government have grown.

Reconstructing The Contours Of The Copyright Originality And Idea-Expression Doctrines Regarding The Right To Deny Access To Works, Michael D. Murray

Law Faculty Scholarly Articles

Access to innovative scientific, literary, and artistic content has never been more important to the public than now, in the digital age. Thanks to the digital revolution carried out through such means as super-computational power at super-affordable prices, the Internet, broadband penetration, and contemporary computer science and technology, the global, national, and local public finds itself at the convergence of unprecedented scientific and cultural knowledge and content development, along with unprecedented means to distribute, communicate, and access that knowledge. This Article joins the conversation on the Access-to-Knowledge, Access-to-Medicine, and Access-to-Art movements by asserting that the copyright restrictions affecting knowledge, innovation, …

Contractor Evaluations In The Contractor Selection Process, Timothy R. B. Taylor, Roy E. Sturgill, Maegan Mcdowell, Alexa Deep, Paul M. Goodrum

Kentucky Transportation Center Research Report

The current contractor evaluation system in use within the Kentucky Transportation Cabinet is based on the contractor evaluation system developed as part of SPR 212-00 “Quality Based Prequalification of Contractors.” This system relies on average performance ratings from the completion of the Contractor’s Performance Report at the conclusion of every project or annually for multi-year projects. The contractor’s average performance rating is weighted based on the dollar value of work performed across all projects for the year. The average performance rating is used in the computation of the Contractor’s Maximum Eligibility Amount in accordance with 603 KAR 2:015, Section 5. …

From Blue To Green: The Development And Implementation Of A Horticultural Therapy Program For Residents Of A Battered Women’S Shelter, Claire M. Renzetti, Diane R. Follingstad, Diane Fleet

CRVAW Faculty Presentations

No abstract provided.

Co2 Recycling Using Microalgae For The Production Of Fuels, Michael H. Wilson, John Groppo, Andrew Placido, S. Graham, S. A. Morton Iii, Eduardo Santillan-Jimenez, Aubrey Shea, Mark Crocker, Czarena Crofcheck, Rodney Andrews

Center for Applied Energy Research Faculty and Staff Publications

CO₂ capture and recycle using microalgae was demonstrated at a coal-fired power plant (Duke Energy’s East Bend Station, Kentucky). Using an in-house designed closed loop, vertical tube photobioreactor, Scenedesmus acutus was cultured using flue gas as the CO₂ source. Algae productivity of 39 g/(m² day) in June–July was achieved at significant scale (18,000 L), while average daily productivity slightly in excess of 10 g/(m² day) was demonstrated in the month of December. A protocol for low-cost algae harvesting and dewatering was developed, and the conversion of algal lipids—extracted from the harvested biomass—to diesel-range hydrocarbons via catalytic …

Characterization Of Glomerella Strains Recovered From Anthracnose Lesions On Common Bean Plants In Brazil, Quélen L. Barcelos, Joyce M.A. Pinto, Lisa J. Vaillancourt, Elaine A. Souza

Plant Pathology Faculty Publications

Anthracnose caused by Colletotrichum lindemuthianum is an important disease of common bean, resulting in major economic losses worldwide. Genetic diversity of the C. lindemuthianum population contributes to its ability to adapt rapidly to new sources of host resistance. The origin of this diversity is unknown, but sexual recombination, via the Glomerella teleomorph, is one possibility. This study tested the hypothesis that Glomerella strains that are frequently recovered from bean anthracnose lesions represent the teleomorph of C. lindemuthianum. A large collection of Glomerella isolates could be separated into two groups based on phylogenetic analysis, morphology, and pathogenicity to beans. Both …

Plenary Session, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Using Microbial-Source Tracking to Investigate Sources of Fecal Contamination in the South Fork Little River Basin, Hopkinsville, Kentucky, Angie Crain and Mac Cherry, USGS Kentucky Water Science Center, Louisville, KY
• Application of Nitrogen and Oxygen Isotopes in Tracing Nitrate Sources in the South Fork Little River Basin, Hopkinsville, Kentucky, Angie Crain and Mac Cherry, USGS Kentucky Water Science Center, Louisville, KY
• Kentucky StreamStats Water-Resources Web Application: Enhancements and Implementation of Selected Low- and Mean-Flow Estimating Equations, Gary Martin, USGS Kentucky Water Science Center, Louisville, KY
• Kentucky Division of Water's Risk Communication Toolbox, Annemarie Cason and Kristen …

Proceedings Of 2014 Kentucky Water Resources Annual Symposium, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

This symposium was planned and conducted as a part of the state water resources research institute annual program that is supported by Grant/Cooperative Agreement Number G11AP20081 from the United States Geological Survey. The contents of this proceedings document and the views and conclusions presented at the symposium are solely the responsibility of the individual authors and presenters and do not necessarily represent the official views of the USGS or of the symposium organizers and sponsors. This publication is produced with the understanding that the United States Government is authorized to reproduce and distribute reprints for government purposes.

Community Health Workers: Implementing A System In Kentucky, S. Makwana, Frances J. Feltner, J. Chamness, C. White, S. Mayfield Gibson

Center of Excellence in Rural Health Presentations

A Community Health Worker (CHW) is a frontline public health worker who is a trusted member of and/or has an unusually close understanding of the community served. This trusting relationship enables the CHW to serve as a liaison/link/intermediary between health/social services and the community to facilitate access to services and improve the quality and cultural competence of service delivery. A CHW also builds individual and community capacity by increasing health knowledge and self-sufficiency through a range of activities such as outreach, community education, informal counseling, social support and advocacy. (American Public Health Association)

Recognized as professional members of the health …

Use Of Synthetic Isoprenoids To Target Protein Prenylation And Rho Gtpases In Breast Cancer Invasion, Min Chen, Teresa Knifley, Thangaiah Subramanian, H. Peter Spielmann, Kathleen L. O'Connor

Molecular and Cellular Biochemistry Faculty Publications

Dysregulation of Ras and Rho family small GTPases drives the invasion and metastasis of multiple cancers. For their biological functions, these GTPases require proper subcellular localization to cellular membranes, which is regulated by a series of post-translational modifications that result in either farnesylation or geranylgeranylation of the C-terminal CAAX motif. This concept provided the rationale for targeting farnesyltransferase (FTase) and geranylgeranyltransferases (GGTase) for cancer treatment. However, the resulting prenyl transferase inhibitors have not performed well in the clinic due to issues with alternative prenylation and toxicity. As an alternative, we have developed a unique class of potential anti-cancer therapeutics called …

Inactivation Of The Host Lipin Gene Accelerates Rna Virus Replication Through Viral Exploitation Of The Expanded Endoplasmic Reticulum Membrane, Chingkai Chuang, Daniel Barajas, Jun Qin, Peter D. Nagy

Plant Pathology Faculty Publications

RNA viruses take advantage of cellular resources, such as membranes and lipids, to assemble viral replicase complexes (VRCs) that drive viral replication. The host lipins (phosphatidate phosphatases) are particularly interesting because these proteins play key roles in cellular decisions about membrane biogenesis versus lipid storage. Therefore, we examined the relationship between host lipins and tombusviruses, based on yeast model host. We show that deletion of PAH1 (phosphatidic acid phosphohydrolase), which is the single yeast homolog of the lipin gene family of phosphatidate phosphatases, whose inactivation is responsible for proliferation and expansion of the endoplasmic reticulum (ER) membrane, facilitates robust RNA …