Digital Commons Network^™

The Toxoplasma Gondii Protein Rop2 Mediates Host Organelle Association With The Parasitophorous Vacuole Membrane, Anthony P. Sinai, Keith A. Joiner

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Toxoplasma gondii replicates within a specialized vacuole surrounded by the parasitophorous vacuole membrane (PVM). The PVM forms intimate interactions with host mitochondria and endoplasmic reticulum (ER) in a process termed PVM–organelle association. In this study we identify a likely mediator of this process, the parasite protein ROP2. ROP2, which is localized to the PVM, is secreted from anterior organelles termed rhoptries during parasite invasion into host cells. The NH₂-terminal domain of ROP2 (ROP2hc) within the PVM is exposed to the host cell cytosol, and has characteristics of a mitochondrial targeting signal. In in vitro assays, ROP2hc is …

Protection Against Traumatic Brain Injury, Stephen William Scheff

Sanders-Brown Center on Aging Faculty Patents

The present invention relates to therapeutic uses of cyclosporin A to reduce adverse effects of neural injury.

Forage News [2001-07], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Robinson Station Celebrates 75^TH Anniversary
• Economic Benefits of Stockpiled Fescue on Two Northeast Arkansas Farms
• Performance of Cattle on “HIMAG” Tall Fescue Infected with Novel Endophytes
• Determination of Pregnancy Outcome of Mares Grazed on a Toxic-Free Endophyte-Infected Tall Fescue (MaxQ)
• Cutting Height to Maximize Yield and Quality
• Sampling Medium Rectangular Bales: How Many Cores are Enough?
• Upcoming Events

Influence Of Inoculum Size On Phytase Production And Growth In Solid-State Fermentation By Aspergillus Niger, Chundakkadu Krishna, Sue E. Nokes

Biosystems and Agricultural Engineering Faculty Publications

Solid–state fermentation is experiencing renewed interest for industrial enzyme production. Previous studies on the effects of fungal inoculum size on product yield have focused on spore inoculum. However, some organisms require vegetative inocula. This study investigated the effects of initial inoculum colony age, vegetative inoculum size, and duration of fermentation on the production of fungal biomass and phytase in solid–state fermentation using Aspergillus niger grown on wheat bran and soy meal. Initial inocula from 7– and 14–day–old potato dextrose agar (PDA) plates were used to study the effect of inoculum colony age in liquid culture and its further influence on …

Public Involvement In Highway Improvement: A Comparison Of Three Different Visualization Modes For A Case Study In Central Kentucky, Joel Brumm, Keiron Bailey, Ted H. Grossardt

Kentucky Transportation Center Faculty and Researcher Publications

Public involvement in highway improvement presents a set of complex problems involving many stakeholders.Since visualizations are increasingly regarded as essential tools in this process, a clearer understanding of the specific merits of advanced visualization techniques and their potential contributions must be developed.As a step towards integrating visualizations into an improved public planning paradigm termed Structured Public Involvement (SPI), this paper investigates the utility and performance of three visualization modes, termed 2D, 3D and VR (Virtual Reality), for a case study highway in Central Kentucky.

Visualization scenarios were designed and engineered according to the principle of elemental decomposition.Using a combination …

Isolated Spinach Ribulose-1,5-Bisphosphate Carboxylase/Oxgenase Large Subunit Ε N-Methyltransferase And Method Of Inactivating Ribulose-1,5-Bishosphatase Ε N-Methyltransferase Activity, Robert L. Houtz

Horticulture Faculty Patents

The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS)^εN-methyltansferase (protein methylase III or Rubisco LSMT) from a plant which has a des(methyl) lysyl residue in the LS is disclosed. In addition, the full-length cDNA clones for Rubisco LSMT are disclosed. Transgenic plants and methods of producing same which have the Rubisco LSMT gene inserted into the DNA are also provided. Further, methods of inactivating the enzymatic activity of Rubisco LSMT are also disclosed.

Comparison Of The Quantification Of Caffeine In Human Plasma By Gas Chromatography And Elisa, A. B. Carregaro, W. E. Woods, Thomas Tobin, A. Queiroz-Neto

Veterinary Science Faculty Publications

In the present study we evaluated the precision of the ELISA method to quantify caffeine in human plasma and compared the results with those obtained by gas chromatography. A total of 58 samples were analyzed by gas chromatography using a nitrogen-phosphorus detector and routine techniques. For the ELISA test, the samples were diluted to obtain a concentration corresponding to 50% of the absorbance of the standard curve. To determine whether the proximity between the I₅₀ of the standard curve and that of the sample would bring about a more precise result, the samples were divided into three blocks according …

Forage News [2001-05], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Mailing List Update
• KFGC Summer Field Day Set for June 19
• Hackley Wins National Forage Spokesperson Contest
• Special Dairy-Beef Grazing School Set for June 12-14
• National Forage Testing Association Listing of Hay Probes
• Reducing the Risk of Hay Fires
• Upcoming Events

Pride Water Quality Assessment Report: Iv. Nutrient Assessment, Kentucky Water Resources Research Institute, University Of Kentucky

KWRRI Research Reports

The efficient utilization of federal funds in improving the water quality and aquatic habitat of the region requires a mechanism for assessing and evaluating the impacts of the proposed and ongoing projects as well as some mechanism for prioritizing the allocation of additional funds. In order to evaluate the effectiveness of these projects it is important to provide a formal monitoring and assessment program based on sound scientific principles. This report provides an initial 10 year baseline assessment of the existing water quality conditions in the 40 county PRIDE region for the purpose of evaluating the impacts of the PRIDE …

Use Of The Naturally-Occuring Quinones Thymoquinone And Dithymoquinone As Antineoplastic And Cytotoxic Agents, Peter A. Crooks, David R. Worthen, Omar A. Ghosheh

Pharmaceutical Sciences Faculty Patents

Nigella sativa derivatives, thymoquinone (TM) and dithymoquinone (DIM) are used in treatment of parental and multi-drug resistant human cancers.

Childhood And Child Life. Disclosure Interviews Jo Boyden, Margo Kleinfeld

disClosure: A Journal of Social Theory

No abstract provided.

Cyber Children: Discursive And Subjective Practices In The Palace, Angela Thomas

disClosure: A Journal of Social Theory

No abstract provided.

Forage News [2001-04], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Sign Up Now for 2001 Spring Kentucky Grazing School
• Clover Selection Guidelines
• Alfalfa Seedings up in 2000
• Roundup Ready Alfalfa-A Progress Report
• Distance Cattle Travel to Water Affects Pasture Utilization Rate
• Hybrid Alfalfa Promoted in Popular Press
• Disk vs. Sicklebar Mowers
• Controlled Grazing
• Upcoming Events

[Review Of] The British Beat Boom, 1963-66, Robert A. Aken

Library Faculty and Staff Publications

No abstract provided.

Aluminium Toxicokinetics: An Updated Minireview, Robert A. Yokel, Patrick J. Mcnamara

Pharmaceutical Sciences Faculty Publications

This MiniReview updates and expands the MiniReview of aluminium toxicokinetics by Wilhelm et al. published by this journal in 1990. The use of ²⁶Al, analyzed by accelerator mass spectrometry, now enables determination of Al toxicokinetics under physiological conditions. There is concern about aluminium in drinking water. The common sources of aluminium for man are reviewed. Oral Al bioavailability from water appears to be about 0.3%. Food is the primary common source. Al bioavailability from food has not been adequately determined. Industrial and medicinal exposure, and perhaps antiperspirant use, can significantly increase absorbed aluminium. Inhalation bioavailability of airborne soluble Al …

Alfalfa As A Cash Hay Crop, John Nowak

Kentucky Alfalfa and Stored Forage Conference

Over the years we have all learned of the great potential alfalfa has as a forage crop in Kentucky. We have seen it used as hay, haylage and even pasture. Many aspects of our agri-business community have alfalfa as an important part of their business.

Alfalfa For Beef Cattle, W. Roy Burris

Kentucky Alfalfa and Stored Forage Conference

Alfalfa is sometimes said to be "too good" for beef cattle. Although it's nutritional value may sometimes exceed beef cows' needs, to dismiss it's potential for beef cattle would be a mistake. Alfalfa can be useful as a harvested feed or as a grazing crop.

Alfalfa For Dairy Cattle, Donna M. Amaral-Phillips

Kentucky Alfalfa and Stored Forage Conference

Alfalfa is known as the queen of the forage crops and for good reasons. Alfalfa is an excellent forage for dairy cattle because it provides nutrients needed by dairy cows in a package which is highly digestible and cost effective. Alfalfa can be grazed directly by cattle or harvested as hay, haylage, or batage. Dairy farmers will purchase locally-grown hay if it is high quality (RFV greater than 150). When marketing cash hay one needs to understand that the quality of alfalfa greatly impacts milk production and economics of a dairy operation. This impact is felt even when as little …

Alfalfa Hay For Horses, Laurie Lawrence

Kentucky Alfalfa and Stored Forage Conference

Horse owners frequently mention cleanliness, color and cutting when it comes to choosing hay. Cleanliness should be the most important factor in selecting horse hay. Hay that contains dust or mold can inflame the respiratory tract. It is also possible for hay mold to affect other systems in the horse such as the digestive system and liver. Some horses suffer from a chronic respiratory condition that seriously impairs their ability to breathe normally. This condition, commonly known as heaves, worsens when horses are fed moldy or dusty hay. Horses with heaves are exercise intolerant and thus have little value except …

Reducing The Risk Of Bloat, Patty Scharko

Kentucky Alfalfa and Stored Forage Conference

Bloat refers to excessive accumulation of gas in the rumen. Bloat results when an animal can not eructate or "belch up" gases produced in the process of rumen fermentation. The gas may be in the free form or may be mixed with rumen contents in the form of froth. It occurs both on pasture and in feedlots and can be a major cause of death in cattle wherever intensive farming is practiced. Additional losses can include decreased milk production and reduced rate of gain. Although legumes may increase the opportunity for bloat to occur, fear of bloat should not keep …

Grazing Alfalfa: Is It Right For You?, Jimmy C. Henning

Kentucky Alfalfa and Stored Forage Conference

Grazing alfalfa can be a very profitable and valuable practice for many Kentucky farmers. How do you know if this practice will work for you? First, you must be able to grow alfalfa. Second, you must address the concerns that are specific to grazing alfalfa.

Am-Pm Cutting Of Alfalfa, Garry D. Lacefield, Michael Collins, Jimmy C. Henning

Kentucky Alfalfa and Stored Forage Conference

Over the past twenty years of this Conference we have spent considerable time discussing "Alfalfa Harvest Management." We have discussed the importance of stage of maturity and its impact on quality on many occasions. We have presented research on many aspects of hay and haylage management. An overall theme for all the discussions has been "harvest for quality," with emphasis on saving the leaves during the entire harvesting process. Our recommendations have, are, and will continue to be centered around those management practices that will result in the greatest opportunity for preserving alfalfa quality from standing crop to feeding. This …

Safety Impacts Of Rural Road Construction, Kenneth R. Agent, Jerry G. Pigman

Kentucky Transportation Center Research Report

Crash data in Kentucky show that the fatal crash rate on two-lane rural roads is substantially higher than on any other type of road. Improvements have been proposed at some locations on this type of road which involve either upgrading the existing two-lane road or adding lanes resulting in a four-lane road. As part of the public information process, highway officials have been asked to document the previous results of this type of construction. The objectives of this study were to: 1) identify sections of two-lane rural roadways where either the two-lane road had been realigned and reconstructed or additional …

And They're Off: The Legality Of Interstate Pari-Mutuel Wagering And Its Impact On The Thoroughbred Horse Industry, M. Shannon Bishop

Kentucky Law Journal

No abstract provided.

Bone Cutting, Placement, And Cannibalism? Middle Preceramic Mortuary Patterns Of Nanchoc, Northern Peru, Jack Rossen, Tom D. Dillehay

Anthropology Faculty Publications

Mortuary practices of the Middle Preceramic period (ca. 8500-4000 B.P.) are discussed for the Nanchoc region of the upper Zaña Valley, northern Peru. Careful breaking, cutting, and placement of human bones from adult males during the Las Pircas Phase (8500-6000 B.P. ) gave way to more haphazard breakage and discard during the subsequent Tierra Blanca Phase (6000-5000 B.P.). The evidence of cannibalism is considered. Bone breakage, cutting, and possibly cannibalism is believed to have been part of a broader process of ritualization that mitigated the spiritual danger of the transition from hunting-gathering to horticulture.

Este trabajo discute las prácticas mortuorias …

Submerged And Solid-State Phytase Fermentation By Aspergillus Niger: Effects Of Agitation And Medium Viscosity On Phytase Production, Fungal Morphology And Inoculum Performance, Maria Papagianni, Sue E. Nokes, Keith Filer

Biosystems and Agricultural Engineering Faculty Publications

Qualitative relationships between agitation levels and medium viscosity, Aspergillus niger morphology and phytase production were investigated in submerged and solid-state fermentations. Overall phytase production increased with increasing shaker speeds from 150 to 300 rpm, although specific growth rates and phytase production rates were higher at 150 rpm for 72 h from inoculation. Fungal morphology was greatly influenced by agitation with the morphological forms of small pellets and entangled mycelia predominating at 150 rpm, while the free filamentous form was obtained at 300 rpm. Upon inoculation of SSF, increased productivities were obtained from inocula grown at 150 rpm. A shift towards …

Clenbuterol In The Horse: Confirmation And Quantitation Of Serum Clenbuterol By Lc-Ms-Ms After Oral And Intratracheal Administration, A. F. Lehner, J Daniel Harkins, W. Karpiesiuk, W. E. Woods, N. E. Robinson, L. Dirikolu, M. Fisher, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

Clenbuterol is a β₂ agonist/antagonist bronchodilator, and its identification in post-race samples may lead to sanctions. The objective of this study was to develop a specific and highly sensitive serum quantitation method for clenbuterol that would allow effective regulatory control of this agent in horses. Therefore, clenbuterol-d₉ was synthesized for use as an internal standard, an automated solid-phase extraction method was developed, and both were used in conjunction with a multiple reaction monitoring liquid chromatography-tandem mass spectrometry (LC-MS-MS) method to allow unequivocal identification and quantitation of clenbuterol in 2 mL of serum at concentrations as low as 10 …

Basic Blue In East Slavonic, Andrew R. Hippisley

Linguistics Faculty Publications

Russian’s second BLUE term goluboj ‘light blue’ constitutes a well-known exception to the Berlin and Kay basic color-term typology. If other Slavonic languages do not have a second BLUE term, then the special position of Russian requires explanation; if there is evidence pointing to two basic terms for BLUE, we have a second set of data for investigating the evolution of this unusual color system. The languages genetically closest to Russian, Ukrainian and Belarusian, are examined. Findings of the list task, a simple and elegant test for psychological salience of color terms, provide strong evidence that Ukrainian and Belarusian have …

From Sheet Music To Mp3 Files--A Brief Perspective On Napster, Harold R. Weinberg

Kentucky Law Journal

No abstract provided.

Vermont Civil Unions, Full Faith And Credit, And Marital Status, Lewis A. Silverman

Kentucky Law Journal

No abstract provided.