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Quantification Of Dental Fissures Through Occlusal Topography Analysis, Duru Erkan

Biological Sciences Undergraduate Honors Theses

Narrow, deep, and elaborate dental fissures are widely believed to increase the predisposition of enamel to develop carious lesions as these surfaces are thought to be ideal for cariogenic bacteria to adhere to. Therefore, dentists prescribe sealants as a preventative measure for patients with such fissures to avoid tooth decay. Yet to date, there is no objective data supporting the notion that fissure morphology actually affects caries susceptibility, nor is there a quantifiable, clinically practical method to characterize fissure patterns expected to increase risk of caries disease.

In this study, three new methods to quantitatively characterize fissure pattern in mandibular …

Exploring Nitrogen Fixation In Methanosarcina Acetivorans, Rylee Cook

Biological Sciences Undergraduate Honors Theses

As of 2016, the genomes of over sixty species of methanogenic archaea have been sequenced.4 Research on the genomes of methanogens such as M. acetivorans led to the characterization of numerous genes with unknown functions. Genes similar to those of nitrogenase have been shown to possess functions unrelated to nitrogen fixation, such as F430 biosynthesis genes. 5,6,7 My research focused on the uncharacterized gene clusters ma2032-33 and ma1631-33 (Figure 1). These genes have conserved nitrogenase domains: ma2032 has fused nifH and nifD domains, while ma2033 has a nifD domain. The ma1631-33 gene cluster contains two nitrogenase homologs and one iron …

Structural Formation And Functionality Of The Spoiie Protein In Clostridium Difficile, Rachel Siebenmorgen

Biological Sciences Undergraduate Honors Theses

My research efforts were directed toward the structure and functionality of a protein, designated SpoIIE [11], which previously has been shown to regulate C. difficile peptide utilization in addition to sporulation and toxin release. The SpoIIE protein is found in all spore-forming gram-positive bacteria and is a crucial component in activation of transcription factors and septum formation thus affecting the regulation and timing of spore development [11,12]. A truncated form of the SpoIIE protein named SpoIIE∆TM was used in an effort to determine the structure of an important but uncharacterized linker domain of the protein. If the structure and specific …

Examining The Invasion Of Bush Honeysuckle Through A Phylogenetic Analysis, Phoebe Bruffett

Biological Sciences Undergraduate Honors Theses

The mechanisms underlying the continual spread of invasive plants within their respective non-native ranges is a major focal point to invasion research. Many theories have been proposed to understand these invasions, each with different implications for the predicted range for invasive plants. Lonicera maackii provides an exceptional opportunity to examine the effectiveness of these theories to explain the trends of invasive plants. Lonicera maackii is native to eastern Asia, but has invaded much of the eastern United States, presenting a severe threat to the health of forest and other natural areas. Recent analysis of the climatic envelopes uncovered a significant …

Investigating The Potential Role For The Nervous System In Controlling Regeneration In Nematostella Vectensis, Kristen Malir

Biological Sciences Undergraduate Honors Theses

Nematostella vectensis is a marine sea animal that has become a model for developmental and evolutionary research. Included in the phylum Cnidaria, N. vectensis’ was chosen as the model for this research. Not only can this animal go through asexual and sexual reproduction, but it also has the ability to regenerate. Although much research has been put forth in an effort to understand regeneration better, much is still unknown. The underlying mechanisms of regeneration in Cnidaria are illusive; however, studies within vertebrates have shown the substantial role of the nervous system. The objective of this experiment is to test if …

Examining Difference In Social Perceptions Between Women Using Hormonal Contraceptives And Naturally Cycling Women, Caroline B. Johnson

Biological Sciences Undergraduate Honors Theses

The term “stress” refers to a person’s psychological and physiological response to the demands and pressures of the world around them (Farlex, 2021). Past research has shown that stress can have negative side effects on a person’s well-being (Aneshensel et al., 1991; Wunsch et al., 2017; Michie, 2002). Although people experience stress, some people perceive more stress than others. Perceptions are important because the way one understands certain conditions can elicit distinct emotional and physiological responses (Kemeny, 2003). An important factor that has not received a lot of attention is women’s use of hormonal contraceptives. In the United States, 24.4% …

The Synchronization Of Nocturnal Pollinator Behavior And Apple Flower Nectar Production, Madison Jennings

Biological Sciences Undergraduate Honors Theses

Insects perform an essential ecological service by facilitating the pollination of crops for food production worldwide. Recent declines in diurnal bee populations threaten food security and has led to growing concerns about existent pollination methods. Pollination contributions by native nocturnal insects have been documented to occur in a variety of systems, thought to be supported by the plant’s attractant and reward system. If this is the case, pollination of flowers by nocturnal vectors may be influenced by circadian cycles of nectar production and insect activity. To test this hypothesis, we recorded insect abundance of nocturnal pollinators periodically throughout the night …

The Effects Of Time Restricted Feeding On Markers Of Type 2 Diabetes, Brooke Martin

Biological Sciences Undergraduate Honors Theses

Type 2 diabetes is one of the fastest growing chronic diseases in the country, comprising 10% of the American population. Obesity rates are at an all-time high and greatly increase risk factors for developing diabetes. Insulin resistance is the main feature of type 2 diabetes and causes management of the disease to be extremely difficult. Currently there are different possible treatments for type 2 diabetes including medication and dieting. However, diets involving fasting, though high in popularity, do not have a lot of evidence regarding their ability to prevent the development of type 2 diabetes.

Time restricted feeding (TRF) is …

Characterization Of Fgf1/Fgf2 Heterodimer With Mutation R136e, Brett Haley

Biological Sciences Undergraduate Honors Theses

Fibroblast growth factors are a family of heparin binding proteins that are involved in a broad spectrum of physiological functions, such as angiogenesis, cell proliferation, cell differentiation, and wound healing. Fibroblast growth factor 1 (FGF-1) is a member of the FGF superfamily and can bind universally to fibroblast growth factor receptors. Fibroblast growth factor 2 (FGF-2) is similar in structure to FGF-1 but has been shown to have a potent effect on angiogenesis. A physiologically stable dimerization of these two growth factors could prove to induce desirable effects in the instance of wound healing. In this study, we have designed …

Regulation Of The Reaction Between Cytochrome C And Cytochrome C Oxidase In The Mitochondria, Anders Nowell

Biological Sciences Undergraduate Honors Theses

Cytochrome c (Cc) is a multifunction protein that has important life and death functions in the cell. In the electron transport chain (ETC), Cc transfers electrons from cytochrome bc₁ to cytochrome c oxidase (CcO), which helps build the electrochemical gradient that drives ATP synthase. The reaction of Cc with CcO is very important in ETC regulatory processes. Previous research shows phosphorylation sites in Cc that affect the binding with CcO, with measurable effects on k_d, k_f, and K_D. These effects result in changes in mitochondrial membrane potentials, respiration, and reactive oxygen species (ROS) …

The Effects Of Hormonal Contraceptive Use On Perceptions Of Social Stress In Women, Ashton Jones

Biological Sciences Undergraduate Honors Theses

Hormonal contraceptives are widely used due to their efficiency in preventing pregnancy. Although women are often informed of the physical effects of hormonal contraceptives, there is little emphasis on mental effects, such as increased rates of depression and changes in social perception. The current study examined perceptions of social stress among women before beginning hormonal contraceptives, and two months after using hormonal contraceptives. I hypothesized that women would experience increased social stress after beginning hormonal contraceptives, compared to a control group of women who were also assessed twice. Results did not support this hypothesis; there was no difference in interpersonal …

Using Ai To Examine Nocturnal Moth Pollination, Conor Moriarty

Biological Sciences Undergraduate Honors Theses

The importance of insect-mediated agriculture cannot be understated especially in the wake of pollinator population declines. Most research of insect-mediated pollination is focused on diurnal pollinators like the honeybee (Apis mellifera). Deepening our understanding of how other pollinators, such as nocturnal pollinators, can benefit agriculture and ecosystems will be very important as honeybee populations decline. I explored nocturnal moth interactions with flowering apple flowers during their pollination season to better understand how these nocturnal pollinators interact with the plants. To accomplish this, I used a University of Arkansas farm area with a dedicated apple orchard. I gathered my raw data …

Prospective Person Memory In The Case Of Missing Persons: A Coffee Shop Study, Cara Bascom

Psychological Science Undergraduate Honors Theses

Prospective person memory (PPM) is the process of remembering to perform some action after encountering a target individual, such as identifying and reporting a missing person sighting after viewing a missing person alert (Moore et al., 2021). Research has shown that identification rates generally tend to be low in simulated missing person studies (Lampinen & Moore, 2016b). The purpose of the current research is to determine how to improve missing person recognition rates. This project explores the potential effects of using videos in missing person reports as compared to using static images. We also consider differences between rigid and non-rigid …

Relationship Between Pet And Human Separation Anxiety On Owners’ Anxiety And Physical Health, Noah Ross

Psychological Science Undergraduate Honors Theses

Pet ownership has always been thought to be beneficial, but some recent studies have not been consistent with this statement. A possible explanation for this is an unhealthy overdependence that pets and pet owners have developed as a result of the Coronavirus pandemic. In this paper, I examine whether separation anxiety from pets, people, and separation behavior of pets could be predictors of anxiety and physical health. The first prediction was that pet and non pet owners differed demographically. Also, it was hypothesized that separation anxiety from humans, separation anxiety from pets, but not pet separation behaviors predicted anxiety and …

Self-Rated Function And Delayed Onset Muscle Soreness (Doms) Responses To 6-Week Supplementation With Waters With Anti-Inflammatory Capabilities (Wac), Kristin Garner

Health, Human Performance and Recreation Undergraduate Honors Theses

Introduction: Researchers have examined the effects of sedentary lifestyles on diminished health outcomes and are pleading individuals to take on a more active lifestyle to prevent these. However, initial efforts to exercise are often dampened by delayed onset muscle soreness (DOMS) and muscle fatigue. This can greatly reduce exercise adherence, making it difficult for individuals to overcome this temporary discomfort. Background: Research has extensively evaluated nonsteroidal anti-inflammatory drugs (NSAIDs) and fruits as anti-inflammatory supplementation to treat DOMS. Berry-derived essences are more highly concentrated in anti-inflammatory substances than regular fruit juices, but there is minimal research on their effects on exercise …

Impact Of Fear On Interpersonal And Economic Decision-Making, John Wilson

Economics Undergraduate Honors Theses

Fear is one of the most basic, intrinsic, and powerful emotions an individual may experience when faced with known or unknown threats, imminent pressures, or expectations of approaching doom. Fear may allow an individual to act quickly in a fight-or-flight response. Fear can alter both physiological and psychological frameworks to avoid certain calamity. Fear provides motivation to protect oneself or to effectuate altruistic behavior towards others for the greater good. One lesser explored area of research pertaining to fear and its implications is the influence of fear on interpersonal and economic decision-making. Economic volatility can produce both immediate consequences as …

Ghosts And Ethics In The Early Works Of James Joyce, Michael Menase

Graduate Theses and Dissertations

In the last 20 years, critics have contributed new insights into the character development, overall messages, themes, and other literary aspects of James Joyce’s works by focusing on their ethical implications. By following an intertextual method and by performing close readings of Joyce’s texts, I try to fill a gap in the scholarly literature by adding a related focus on ghosts to the conversation about Joyce’s ethics and those of his characters. Focusing on Dubliners and A Portrait of the Artist as a Young Man, I hope to show that the ghosts in Joyce’s corpus motivate characters and readers to …

Reconstructing Bison And Mammoth Migration During The Late Pleistocene And Early Holocene Of Central Texas Using Strontium Isotopes, Joshua John Porter

Graduate Theses and Dissertations

During the Late Pleistocene (LP; past 130,000 years), over two-thirds of large mammal (>45kg) species went extinct globally. While the role of humans is hotly debated, the effect of these extinctions is growing clearer; the extinctions resulted in widespread and lasting faunal community reorganization. However, the impact of these extinctions on dietary and migratory behavior within faunal communities is unknown. Our study examines the impact of the megafaunal extinctions on the dietary and migratory behavior of surviving Bison individuals in Texas using carbon, oxygen, and strontium isotopes. Strontium isotopes are incorporated into mammalian enamel during their tooth development and …

Examining The Effects Of Oral Contraceptive Use On Thermoregulation, Whitley C. Atkins

Graduate Theses and Dissertations

Purpose: The purpose of this investigation was to evaluate the effects of combined (estradiol and progestin) monophasic oral contraceptive pill (OCP) use on thermoregulation. Further, we sought to evaluate OCP use on acute rehydration post-exercise in the heat using recommended rehydration guidelines. Methods: Twelve healthy, aerobically trained (VO2peak = 47.8 ± 4.7 mL/kg/min), long term female oral contraceptive users completed a familiarization trial and two experimental days separated by ≥ 7 days. The two experimental trials were identical except for the hormone dosing phase. One trial was completed during the third week of active pill dosing (ACT) and one during …

Macronutrient Availability Shapes Host Response To Infection And Feeding Behavior, Weston Gerald Perrine

Graduate Theses and Dissertations

Macronutrients in the diet are vital to the physiological processes necessary for an organism to effectively clear a pathogen. Diet can be important to a host’s susceptibility to infection and severity of pathology, though results can vary across host-pathogen systems (Sen et al. 2016). Manipulating the ratio of specific macronutrients in the diet is an effective method to begin understanding how individual macronutrients, rather than food types, have on immune responses. Using an avian host-pathogen system, I explored the effects of dietary macronutrient composition, specifically lipid and protein content, on disease pathology and behavior of canaries (Serinus canaria) infected with …

Post-Obergefell V. Hodges: How Lgbt Contact Can Alter Public Lgbt Policy Positions In The U.S. And Arkansas, Briana Huett

Graduate Theses and Dissertations

The Contact Theory (CT) of attitudinal change has utilized to understand perceptions of minority-group members and the policies that surround them since the 1950s. It has further been used to specifically examine how we form our opinions of LGBT-identifying individuals, the LGBT community, and LGBT policies more generally. However, further evidence is still needed from the CT literature surrounding how this form of contact interacts with individuals’ social identities to determine and alter their LGBT policy positions, how the level of contact with LGBT persons might have differing effects on these positions, and whether LGBT contact holds the same effects …

A Mixed Methods Approach To Context Specific Curriculum Design For Very Small And Small Strawberry Growers In The Southeastern United States, Thomas A. Yeargin

Graduate Theses and Dissertations

Outbreaks of foodborne illness due to fresh produce are a continued threat to both the publichealth and the economy in the United States. Though there are many factors which influence the perpetuation of foodborne pathogens, the inability of the food industry to curtail this issue indicates systemic failure of interventions aimed at improving food safety practices. In this dissertation, we detail the efforts made over the past few decades to provide training to food producers and food handlers as well as recommendations that have been made for improvement based on these studies. By borrowing from more advanced fields of study …

Ultrafiltration Membranes Functionalized With Copper Oxide And Zwitterions For Fouling Resistance, Cannon Hackett, Mojtaba Abolhassani, Lauren Greenlee, Audie K. Thompson

Chemical Engineering Faculty Publications and Presentations

Polymeric membrane fouling is a long-standing challenge for water filtration. Metal/metal oxide nanoparticle functionalization of the membrane surface can impart anti-fouling properties through the reactivity of the metal species and the generation of radical species. Copper oxide nanoparticles (CuO NPs) are effective at reducing organic fouling when used in conjunction with hydrogen peroxide, but leaching of copper ions from the membrane has been observed, which can hinder the longevity of the CuO NP activity at the membrane surface. Zwitterions can reduce organic fouling and stabilize NP attachment, suggesting a potential opportunity to combine the two functionalizations. Here, we coated polyethersulfone …

The Effects Of Metronomic And Maximum-Tolerated Dose Chemotherapy In Colorectal Cancer Angiogenesis: A Combined Approach Using Endoscopic Diffuse Reflectance Spectroscopy And Mrna Expression, Ariel Isaac Mundo Ortiz

Graduate Theses and Dissertations

Colorectal cancer (CRC) continues to be one of the most incident and deadliest types of cancer worldwide. Chemotherapy has proven effective to reduce tumor burden for CRC patients, but there are several disadvantages associated with the use of mainstay maximtolerated dose (MTD) chemotherapeutic strategies. Metronomic chemotherapy (MET) has been developed as an alternative that addresses the shortcomings of maximum-tolerated dose chemotherapy but so far its effectiveness as a neoadjuvant strategy for CRC has not been explored.

This dissertation uses a combined optics and molecular biology approach (using diffuse reflectance spectroscopy and mRNA expression) to study the changes in angiogenesis and …

Characterization Of The Influence Of A Small Molecule Inhibitor On Ras-Related Proteins Interactions, Emilio Duverna

Graduate Theses and Dissertations

The Ras superfamily of small G proteins are involved in cell-signaling processes that, if not regulated, may lead to cell multiplication, apoptosis inhibition, and tumorigenesis. They function as molecular switches, which through GTP/GDP exchange cycle, switch on or off cellular activities. Overexpression and/or hyperactivity of these proteins have been linked to many diseases including various cancers. CDC42, a member of the Rho subfamily of the Ras superfamily of small G proteins, participates in the regulation of many cellular processes including cell adhesion, mitosis, and cytoskeletal rearrangements. CDC42 binds to and activates many effector proteins including CDC42-activated kinase (ACK). Abnormal activities …

Diffusive Dynamics Of Biological Macromolecules In The Cytoplasm Of Live Bacteria, Asmaa Sadoon

Graduate Theses and Dissertations

Bacteria are small organisms that play important roles in our bodies and in maintaining our surrounding environment. However, bacterial infections are considered as one of the health diseases that threatens the lives of humans. Resistance of bacteria to antibiotics has made treating bacterial infections more challenging. With the emergence of many developed optical techniques, it has become possible to study various biological mechanisms of molecules inside these small organisms with high resolution and precise localization. In this research, single particle tracking photoactivation localization microscopy (sptPALM) technique was used to investigate the dynamics of fluorescent histonelike nucleoid-structuring proteins (H-NS) in live …

Cyanobacterial And Microcystin Response To Nutrient Additions At Lake Fayetteville Throughout The 2021 Growing Season, Lillie Haddock

Graduate Theses and Dissertations

Harmful Algal Blooms (HABs) are becoming a global concern due to their increasing distribution, frequency, intensity, and the occurrence of toxins. While it is known that eutrophication influences algal blooms, there is less known about what triggers these HABs to produce toxins, especially microcystin. In this study, we conducted 21 community bioassays at Lake Fayetteville, a hypereutrophic reservoir in Fayetteville, Arkansas, from April-November 2021 to examine how the addition of phosphorous and nitrogen influence cyanobacteria concentrations, microcystin concentrations, and microcystin toxin production. These experiments included a control, nitrogen (1.0 mg/L as KNO3), low phosphorus (0.025 mg/L as K2HPO4), high phosphorus …

Multi-Trophic Biodiversity Increases With Increasing Structural Complexity Of Forest Canopy, Ayanna St. Rose

Graduate Theses and Dissertations

Understanding the effects of forest canopy structural complexity on multi-trophic diversity is critical for conserving biodiversity and managing land sustainably. But multi-trophic diversity is often ignored when making decisions about land management due to lack of cost- and time-effective methods to evaluate it. Here, we explored a new method based on widely available remote sensing data to quantify canopy structural complexity and its relationships with multi-trophic biodiversity at landscape scale using 32 forested sites of the National Ecological Observatory Network. We investigated the influence of vertical and horizontal structural complexity of forest canopy on multi-trophic (primary producers, herbivores (beetles), omnivores …

Development Of A Long-Read Sequencing Protocol To Assess The Precision And Efficacy Of Gene Editing For Duchenne Muscular Dystrophy, Landon Andrew Burcham

Graduate Theses and Dissertations

This work establishes a method for assessing on-target precision due to CRISPR-Cas9 gene editing, especially within the context of exon skipping therapy for Duchenne Muscular Dystrophy. The proposed method utilizes an Oxford nanopore long-read sequencing approach to sequence amplified regions of DNA that have been edited using CRISPR-Cas9. NIH3T3 and C2C12 cell lines were treated with a dual-guide CRISPR-Cas9 system, that targets and deletes exon 23 from the DMD gene in mouse samples. Deletion PCR revealed deletion of exon 23 in both DNA and cDNA samples. Additionally, sequencing using Oxford Nanopore revealed targeted exon 23 deletion as the most prevalent …

Tooth Crown Morphology And Testosterone Transfer In Female Members Of Opposite-Sex Dizygotic Twin Pairs, Randall Feezell

Graduate Theses and Dissertations

The twin testosterone transfer (TTT) hypothesis posits that females gestated with male co-twins develop more masculine phenotypes due to in-utero androgen exposure. Research has shown co-gestational effects to be associated with increased deciduous and permanent tooth size in females belonging to opposite-sex dizygotic twin pairs (OSF) as compared with females belonging to same-sex monozygotic (MZF) and dizygotic (SSF) twin pairs and female siblings. This study evaluates whether the TTT hypothesis explains patterns of dental morphological variation, namely differences between OSF and other females (SSF, MZF, female siblings) in a contemporary sample that includes both deciduous and permanent data. This work …