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Full-Text Articles in Entire DC Network
Colchicine-Induced Tetraploidy And Changes In Allele Frequencies In Colchicine-Treated Populations Of Diploids Assessed With Rapd Markers In Gossypium Arboreum L., Saeed Rauf, Iftikhar Ahmad Khan, Faroog Ahmad Khan
Colchicine-Induced Tetraploidy And Changes In Allele Frequencies In Colchicine-Treated Populations Of Diploids Assessed With Rapd Markers In Gossypium Arboreum L., Saeed Rauf, Iftikhar Ahmad Khan, Faroog Ahmad Khan
Turkish Journal of Biology
Two cultivars of cotton G. arboreum L. cv. FDH-228 and HK-113 were treated with colchicine solution (1%) with 3 methods of application. Colchicine application at the seedling stage on shoot apex was successful. Tetraploid plants of arboreum (4x = 2n = 52) had larger vegetative, reproductive parts, but less frequent stomata and higher optical density of DNA than their normal diploid counterparts (2n = 26). Results also showed that individuals that escaped the duplicating effects of colchicine although treated, presented significant point mutagenic effects. Randomly Amplified Polymorphic DNA markers were used to evaluate diversity and similarities in populations treated with …
Nucleic Acid Extraction From Clinical Specimens For Pcr Applications, Ashabil Aygan
Nucleic Acid Extraction From Clinical Specimens For Pcr Applications, Ashabil Aygan
Turkish Journal of Biology
Polymerase chain reaction (PCR) has been widely used due to its high specificity, sensitivity, and rapid turn-around time. However, the major limitation of PCR based diagnosis is inhibition of the reaction caused by a variety of components within clinical specimens. The demand for PCR diagnosis in medical microbiology has highlighted the need for efficient methods of nucleic acid extraction. Although there has been progress in the simplification and purification of microbial nucleic acids, many researchers' procedures are still inconvenient for routine use with any specimen and a universal method has not been devised yet. In this review, some of the …
Acute Effects Of Trichloroacetic Acid On Serum Enzyme Levels And Erythrocyte Carbonic Anhydrase Activity In Rats, Hali̇t Demi̇r, İsmai̇l Çeli̇k
Acute Effects Of Trichloroacetic Acid On Serum Enzyme Levels And Erythrocyte Carbonic Anhydrase Activity In Rats, Hali̇t Demi̇r, İsmai̇l Çeli̇k
Turkish Journal of Biology
The aim of the present study was to investigate the effects of a sublethal dose of trichloroacetic acid (TCA) on serum enzyme levels and erythrocyte carbonic anhydrase (CA) activity in rats under laboratory conditions. Ten Sprague-Dawley albino rats were divided in 2 groups of 5 rats. A 200 mg/kg dose of TCA was administered intraperitoneally to the 5 rats in the treatment group. An equal amount of saline solution was injected into the control group. Serum enzyme levels and erythrocyte CA activity of the rats were measured 1, 3, and 6 h after treatment. According to the results, while the …
Characterization Of Rhizobium Sp. Isolated From Bean, Çi̇ğdem Küçük, Meri̇h Kivanç, Engi̇n Kinaci
Characterization Of Rhizobium Sp. Isolated From Bean, Çi̇ğdem Küçük, Meri̇h Kivanç, Engi̇n Kinaci
Turkish Journal of Biology
Thirty nodule isolates from bean (Phaseolus vulgaris L.) grown in Eskişehir were studied for their physiological and biochemical characteristics. Most isolates produced abundant extracellular polysaccharides, tolerated high salt concentration (5% NaCl), grew at a temperature of 42 °C, and synthesized melanin. They were able to grow at pHs ranging from 3.5 to 9.0. The majority of the isolates showed an intrinsic resistance to the antibiotics chloramphenicol (20 and 50 µg ml^{-1}), erythromycin (30 µg ml^{-1}), kanamycin (10 µg ml^{-1}), and streptomycin (40, 80, and 100 µg ml^{-1}). Plasmid DNA profiles of the isolates were identified and it was determined that …
Evidence For The Presence Of A Second Electron Donor For The Cytoplasmic Thioredoxins In The Yeast Saccharomyces Cerevisiae, Ahmet Koç, Hüseyi̇n Ç. Karakaya, Ercan S. Ünlü
Evidence For The Presence Of A Second Electron Donor For The Cytoplasmic Thioredoxins In The Yeast Saccharomyces Cerevisiae, Ahmet Koç, Hüseyi̇n Ç. Karakaya, Ercan S. Ünlü
Turkish Journal of Biology
In yeast, the cytoplasmic thioredoxin system is composed of NADPH, thioredoxin reductase-1 (TRR1) and 2 thioredoxin genes (TRX1, TRX2). In this study, using yeast knockout mutants for TRR1, TRX1 and TRX2 genes, the role of the thioredoxin system in methionine sulfoxide reduction was investigated. Cells lacking both TRX1 and TRX2 genes simultaneously were not able to reduce methionine sulfoxides to methionine; however, mutants missing the TRR1 gene were able to reduce methionine sulfoxides to methionine, which showed that electrons could be transferred from NADPH to thioredoxins in the absence of TRR1. Similar results were observed for 3-phosphoadenosine 5-phosphosulfate reduction in …
Rnf Genes In Purple Sulfur Bacterium Allochromatium Vinosum, H. Benan Di̇nçtürk, Volkan Demi̇r
Rnf Genes In Purple Sulfur Bacterium Allochromatium Vinosum, H. Benan Di̇nçtürk, Volkan Demi̇r
Turkish Journal of Biology
Allochromatium vinosum is a photosynthetic, diazotrophic purple sulfur bacterium that oxidizes reduced sulfur compounds hydrogen sulfide, elemental sulfur and thiosulfide. In this article, we report the presence of rnf genes in Allochromatium vinosum, some of which have been reported to take part in nitrogen fixation in some species.
Antimicrobial Activity Of Palustriella Commutata (Hedw.) Ochyra Extracts (Bryophyta), Semra İlhan, Fi̇li̇z Savaroğlu, Ferdağ Çolak, Cansu Fi̇li̇k İşçen, Fatma Zerri̇n Erdemgi̇l
Antimicrobial Activity Of Palustriella Commutata (Hedw.) Ochyra Extracts (Bryophyta), Semra İlhan, Fi̇li̇z Savaroğlu, Ferdağ Çolak, Cansu Fi̇li̇k İşçen, Fatma Zerri̇n Erdemgi̇l
Turkish Journal of Biology
The present study describes the antimicrobial activity of 2 extracts of Palustriella commutata (Hedw.) Ochyra, collected from the Sündiken Mountains, Eskişehir, Turkey. These extracts were first prepared with acetone and methanol solvents under reflux. The antimicrobial activity of the extracts was then assessed using the disc diffusion method against 11 bacteria, 1 yeast, and 8 moulds. The antimicrobial test results revealed that the acetone extract had a potential activity against 9 test bacteria. While some gram-positive bacteria tested were sensitive (Bacillus mycoides, B. cereus, B. subtilis, and Micrococcus luteus), all gram-negative bacteria tested (Klebsiella pneumoniae, Yersinia enterocolitica, Pseudomonas aeruginosa, Escherichia …
Hill Reaction, Photosynthesis And Chlorophyll Content In Non-Sugar-Producing (Turnip, Brassica Rapa L.) And Sugar-Producing (Sugar Beet, Beta Vulgaris L.) Root Crop Plants, Manzer H. Siddiqui, M.Masroor A. Khan, M. Nasir Khan, Firoz Mohammad, M. Naeem
Hill Reaction, Photosynthesis And Chlorophyll Content In Non-Sugar-Producing (Turnip, Brassica Rapa L.) And Sugar-Producing (Sugar Beet, Beta Vulgaris L.) Root Crop Plants, Manzer H. Siddiqui, M.Masroor A. Khan, M. Nasir Khan, Firoz Mohammad, M. Naeem
Turkish Journal of Biology
A pot experiment was conducted at the Botany Department, A.M.U., Aligarh, on 2 root crop plants, viz. turnip (Brassica rapa L.), non-sugar-producing and sugar beet (Beta vulgaris L.), sugar-producing, to compare their physiological activity (Hill activity, photosynthesis and chlorophyll content). The seeds of each plant were sown in 20 pots (80% soil + 20% FYM) separately. Two samplings were done at 125 and 140 days (after sowing). Hill activity was measured spectrophotometrically using DCPIP indicator and photosynthesis was assayed by LICOR-6200 Photosynthesis System. Sugar beet gave the maximum leaf-area, Chl a/b ratio, stomatal conductance and photosynthesis rate and Hill activity …
Non-Histone Proteins And The Mediator Complex Are Essential For The Transcription Of Hxt2 And Hxt4 Genes In Saccharomyces Cerevisiae, Si̇nem Güzelvardar, Sezai̇ Türkel
Non-Histone Proteins And The Mediator Complex Are Essential For The Transcription Of Hxt2 And Hxt4 Genes In Saccharomyces Cerevisiae, Si̇nem Güzelvardar, Sezai̇ Türkel
Turkish Journal of Biology
Transcriptional activation of HXT2 and HXT4 genes require rapid reorganization of the chromatin structure on the promoter regions of these genes. In this study, it was found that the transcription of HXT2 and HXT4 genes decreased 4- to 7-fold in the yeast strain that does not contain the non-histone proteins 6A and 6B (Nhp6A/B). In addition, results of this research indicated that Nhp6A/B are required for the rapid derepression and activation of HXT2 and HXT4 transcription upon glucose signaling. Furthermore, it was found that Med2p, which provides the connection between the transcriptional activators and the basal transcription factors, is also …
Antioxidant And Free Radical Scavenging Properties Of Twelve Traditionally Used Indian Medicinal Plants, Farrukh Aqil, Iqbal Ahmad, Zafar Mehmood
Antioxidant And Free Radical Scavenging Properties Of Twelve Traditionally Used Indian Medicinal Plants, Farrukh Aqil, Iqbal Ahmad, Zafar Mehmood
Turkish Journal of Biology
The methanolic crude extracts of 12 traditionally used Indian medicinal plants were screened for their antioxidant and free radical scavenging properties using a-tocopherol and butylated hydroxy toluene (BHT) as standard antioxidants. Antioxidant activity was measured by ferric thiocyanate (FTC) assay and compared with the thiobarbituric acid (TBA) method. Free radical scavenging activity was evaluated using diphenyl picryl hydrazyl (DPPH) radicals. The overall antioxidant activity of Lawsonia inermis was the strongest, followed in descending order by Ocimum sanctum, Cichorium intybus, Piper cubeba, Punica granatum, Allium sativum, Delonix regia, Terminalia chebula, Terminalia bellerica, Mangifera indica, Camellia sinensis, and Trigonella foenum-graecum. Seven plants, …
Antibacterial Activity Of Four Plant Extracts Used In Palestine In Folkloric Medicine Against Methicillin-Resistant Staphylococcus Aureus, Bassam Abu-Shanab, Ghaleb Mohammad Adwan, Naser Jarrar, Awni Abu-Hijleh, Kamel Adwan
Antibacterial Activity Of Four Plant Extracts Used In Palestine In Folkloric Medicine Against Methicillin-Resistant Staphylococcus Aureus, Bassam Abu-Shanab, Ghaleb Mohammad Adwan, Naser Jarrar, Awni Abu-Hijleh, Kamel Adwan
Turkish Journal of Biology
Ethanolic and hot water extracts from 4 different plant species used in Palestine in popular medicine for the treatment of several ailments of microbial and non-microbial origin were evaluated for potential antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA). Both water and ethanol extracts of Mentha longifolia, Melissa officinalis and Rosa damascena were effective on MRSA. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of the ethanolic extract of M. longifolia and M. officinalis were in the range of 3.125 to 12.50 mg/ml and 12.50 to 25.00 mg/ml, respectively. The ethanolic extract with the greatest antimicrobial activity was …
Effectiveness Of The Entomopathogenic Nematodes Heterorhabditis Bacteriophora And Steinernema Feltiae Against Tenebrio Molitor (Yellow Mealworm) Larvae In Different Soil Types At Different Temperatures, Alper Susurluk
Turkish Journal of Biology
The efficiency of the entomopathogenic nematodes Steinernema feltiae Tur-S3 and Heterorhabditis bacteriophora Tur-H2, isolated in Turkey, against larvae of Tenebrio molitor L. was investigated in different soil type and temperature conditions. Sterilized and non-sterilized silver sand, clay-loam soil, and compost soil were tested, each at 12, 18, and 24 ºC. Temperature had the greatest effect on the mortality of T. molitor larvae caused by both nematode species. The efficiency of the 2 nematodes was greater in sterile than in nonsterile conditions, and was greater in sandy soils than in clay soils. The results showed that Steinernema feltiae was more efficient …
The Relationship Between Seasonal Variation In Phytoplankton And Zooplankton Densities In Hirfanlı Dam Lake (Kırşehir, Turkey), Tülay Baykal, Selahatti̇n Salman, İlkay Açikgöz
The Relationship Between Seasonal Variation In Phytoplankton And Zooplankton Densities In Hirfanlı Dam Lake (Kırşehir, Turkey), Tülay Baykal, Selahatti̇n Salman, İlkay Açikgöz
Turkish Journal of Biology
Phytoplankton and zooplankton densities in Hirfanlı Dam Lake were studied between July 1999 and June 2000. In addition, phytoplankton biomass was measured using chlorophyll a concentration. The density of Cladocera at the surface and that of Rotifera at the bottom were low in the summer months. For phytoplankton, Cyclostephanos dubius (Fricke) Round was dominant. The variation in the density of organisms did not follow a known pattern. Species composition, seasonal distribution and species density variations show that the lake was advanced mesotrophic. The lake was alkaline and, from time to time, there was an odor in the littoral regions where …
Karyological Observation Of Red Mullet (Mullus Barbatus Linnaeus, 1758), Serkan Saygun, İsmi̇han Karayücel, Recep Bi̇rcan
Karyological Observation Of Red Mullet (Mullus Barbatus Linnaeus, 1758), Serkan Saygun, İsmi̇han Karayücel, Recep Bi̇rcan
Turkish Journal of Biology
The number and structure of red mullet chromosomes (Mullus barbatus Linnaeus, 1758) were investigated and the species karyotype was analyzed. Specimens were obtained by trawl from the coast of Zonguldak, Turkey. A colchicine solution, 0.1 mg/g of body weight, was intramuscularly injected dorsally, and intraperitoneally injected ventrally. Slides were prepared from gill and fin epithelial tissues. Results of the conventional staining method showed that this species has 2n = 44 diploid chromosomes.
Antibacterial Effects Of Nutraceutical Plants Growing In Palestine On Pseudomonas Aeruginosa, Ghaleb Mohammad Adwan, Bassam Abu-Shanab, Kamel Adwan, Farah Abu-Shanab
Antibacterial Effects Of Nutraceutical Plants Growing In Palestine On Pseudomonas Aeruginosa, Ghaleb Mohammad Adwan, Bassam Abu-Shanab, Kamel Adwan, Farah Abu-Shanab
Turkish Journal of Biology
The antibacterial effects of single and combined plant extracts of water, ethanol and methanol for two nutraceuticals utilized in Palestine were studied against multiple drug resistances Pseudomonas aeruginosa using well diffusion method. These plants are Rhus coriaria and Thymus vulgaris. Combinations of these extracts showed an additive action against this pathogen.
Primary Structure Of The Carbohydrate-Binding Modules In Various Cellulolytic, Thermophilic, Anaerobic, Ethanol-Producing Isolates, Melek Özkan, Gülay Özcengi̇z
Primary Structure Of The Carbohydrate-Binding Modules In Various Cellulolytic, Thermophilic, Anaerobic, Ethanol-Producing Isolates, Melek Özkan, Gülay Özcengi̇z
Turkish Journal of Biology
In the present study, the carbohydrate-binding module (CBM) coding sequences of the cellulosomes of 13 thermophilic, cellulolytic, anaerobic, ethanol-producing bacterial isolates having some variations in their growth and cellulose degradation capacities were amplified by PCR and then sequenced. The sequence analysis of the amplicons revealed that CBMs of 7 of the isolates including the isolate 7-9-1 with the highest capacity of cellulose degradation in solid medium have 100% identity in both nucleotide and amino acid sequences to CipB of Clostridium thermocellum in the compared regions. On the other hand, CBM of the isolate 7-1-2, also having a high cellulolytic activity, …
Effects Of Inoculation With Rhizobium On Seed Yield And Yield Components Of Common Vetch (Vicia Sativa L.), Sebahatti̇n Albayrak, Cafer Sirri Sevi̇may, Özgür Töngel
Effects Of Inoculation With Rhizobium On Seed Yield And Yield Components Of Common Vetch (Vicia Sativa L.), Sebahatti̇n Albayrak, Cafer Sirri Sevi̇may, Özgür Töngel
Turkish Journal of Agriculture and Forestry
The effects of inoculation with Rhizobium leguminosarum on seed yield and yield components of common vetch (Vicia sativa L.) were evaluated under Black Sea Coastal Region conditions, Turkey, in the 2001-2002 and 2002-2003 growing seasons using common vetch cultivars Kubilay, Ürem, Karaelçi, Uludağ, Emir, Çubuk and Nilüfer. The experiment was established as a split block design with 3 replications. Inoculation increased seed yield and all yield components of common vetch cultivars. It was observed that inoculated common vetch cultivars gave higher biological yield (8.5%), seed yield (7.6%), straw yield (10.4%), pod length (25.5%), number of seed per pod (16.2%), number …
Effects Of Different Nitrogen Doses And Row Spacing Applications On Yield And Quality Of Oenothera Biennis L. Grown In Irrigated Lowland And Unirrigated Dryland Conditions, Nazim Şekeroğlu, Menşure Özgüven
Effects Of Different Nitrogen Doses And Row Spacing Applications On Yield And Quality Of Oenothera Biennis L. Grown In Irrigated Lowland And Unirrigated Dryland Conditions, Nazim Şekeroğlu, Menşure Özgüven
Turkish Journal of Agriculture and Forestry
Common evening primrose (Oenothera biennis L., Onagraceae) is a biennial herbaceous forb. It is commercially cultivated in over 15 countries for its seed oil, which contains the essential fatty acids, linoleic and gamma-linolenic acid. The present study was carried out to determine the effects of different nitrogen doses (0, 60, 120 and 180 kg ha^{-1}) and row spacing applications (20, 40 and 60 cm) on yield and quality of Oenothera biennis L. grown in irrigated lowland and unirrigated dryland in the Gukurova conditions region in 2000 and 2001. The field trials were arranged in split-split-plot design with 3 replicates. Some …
Cover And Contents
Turkish Journal of Veterinary & Animal Sciences
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