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Pluronic Pf68 Increases Transfection Efficiency In Electroporationof Mesenchymal Stem Cells, Safa Aydin, Mehmet Emi̇r Yalvaç, Ferruh Özcan, Fi̇kretti̇n Şahi̇n
Pluronic Pf68 Increases Transfection Efficiency In Electroporationof Mesenchymal Stem Cells, Safa Aydin, Mehmet Emi̇r Yalvaç, Ferruh Özcan, Fi̇kretti̇n Şahi̇n
Turkish Journal of Biology
Electroporation is a safe and efficient method to transfect human tooth germ stem cells (hTGSCs), which are mainly mesenchymal stem cells (MSCs). In this study, pluronic F68 (PF68), a nonionic and low-foaming surfactant, was used for the first time to increase the transfection efficiency in electroporation of hTGSCs. Our results revealed that when PF68 was used in the preincubation, electroporation, and recovery medium, it dramatically increased transfection efficiency. Moreover, use of PF68 did not alter the differentiation capacity of hTGSCs, whereas cells electroporated without PF68 had significantly lower expression of Oct-4, pluripotency marker gene, and alkaline phosphatase (ALP) activity upon …
Lycium Barbarum L. (Goji Berry) Fruits Improve Anxiety, Depression-Like Behaviors, And Learning Performance: The Moderating Role Of Sex, Fatma Pehli̇van Karakaş, Hami̇t Coşkun, Kadi̇r Sağlam, Bi̇hter Gökçe Bozat
Lycium Barbarum L. (Goji Berry) Fruits Improve Anxiety, Depression-Like Behaviors, And Learning Performance: The Moderating Role Of Sex, Fatma Pehli̇van Karakaş, Hami̇t Coşkun, Kadi̇r Sağlam, Bi̇hter Gökçe Bozat
Turkish Journal of Biology
The aim of this research was to investigate the effects of the methanol extract obtained from Lycium barbarum fruits on anxiety, depression-like behaviors, and spatial memory in Wistar albino rats. A total of 28 rats were selected, randomly assigned to four experimental groups, and tested by means of the open field and elevated plus maze tests for anxiety-like behaviors, the forced swim test for depression-like behaviors, and the Morris water maze test for spatial memory. The findings demonstrated that in the open field, L. Barbarum-administered rats spent more time at the center and showed more mobility and velocity than controls. …
Purification And Properties Of Lipoxygenase From Opium Poppy Seedlings(Papaver Somniferum L.), Ivana Holková, Frantisek Bilka, Drahomíra Rauová, Lydia Bezáková
Purification And Properties Of Lipoxygenase From Opium Poppy Seedlings(Papaver Somniferum L.), Ivana Holková, Frantisek Bilka, Drahomíra Rauová, Lydia Bezáková
Turkish Journal of Biology
Lipoxygenase (linoleate:oxygen oxidoreductase; EC 1.13.11.12; LOX) is a key enzyme in the signaling pathway leading to the production of biologically active compounds involved in plant growth development and stress responses. In this study the novel LOX enzyme from opium poppy (Papaver somniferum L.) seedlings was purified to electrophoretic homogeneity and characterized. LOX activity during the germination of opium poppy seeds was analyzed, and the highest LOX activity was determined on the fourth day of germination. Opium poppy LOX was purified from 4-day-old poppy seedlings after ammonium sulfate precipitation followed by hydrophobic chromatography (Phenyl-Sepharose CL-4B), ion exchange chromatography (Q-Sepharose), and affinity …
The Effects Of Dna Methyl Transferases On Antiaging Klotho Gene Expression, Eli̇f Çağlayan, Kadi̇r Turan
The Effects Of Dna Methyl Transferases On Antiaging Klotho Gene Expression, Eli̇f Çağlayan, Kadi̇r Turan
Turkish Journal of Biology
Several intracellular metabolic pathways in which multiple genes have a role affect the aging process. One important genetic factor associated with aging is klotho gene. In some recently published studies, klotho gene has also been associated with several types of cancer and identified as a tumor suppressor gene. Therefore, elucidation of the control of klotho gene expression has become more important. Results suggesting that the promoter region of human klotho gene could be epigenetically controlled by DNA methylation have been reported. In contrast, a study revealing whether a change in the expression level of DNMT enzymes in the cells affects …
Inducing Osmotic Stress Leads To Better Genetic Transformation Efficiency In Cotton (Gossypium Hirsutum L.), Surendra Barpete, Allah Bakhsh, Emi̇ne Anayol, Sancar Fati̇h Özcan, Muhammet Cağri Oğuz, Ömer Cem Karakoç, Sebahatti̇n Özcan
Inducing Osmotic Stress Leads To Better Genetic Transformation Efficiency In Cotton (Gossypium Hirsutum L.), Surendra Barpete, Allah Bakhsh, Emi̇ne Anayol, Sancar Fati̇h Özcan, Muhammet Cağri Oğuz, Ömer Cem Karakoç, Sebahatti̇n Özcan
Turkish Journal of Biology
The present study investigated the effect of different salts on cotton shoot regeneration and transformation efficiency. Two-day-old germinating embryos of a local cotton cultivar (SG-125) were pretreated with 50 mM each of NaCl, CaCl2, and KCl for 60 min. The embryo explants were transformed by cocultivation with Agrobacterium tumefaciens strain LBA 4404 harboring a binary plasmid pTF101.1 that carried the insecticidal gene (cry1Ac) under control of wound-inducible promoter (AoPR1) and bilanafos acetyl reductase (bar) gene for plant selection. The salt-pretreated embryos showed maximum response on regeneration MS medium containing 0.50 mg/L 6-benzylaminopurine (BAP) and 0.10 mg/L indole-3-butyric acid (IBA), also …
Potential Therapeutic Effects Of Silymarin And Silymarin-Loaded Solid Lipidnanoparticles On Experimental Kidney Damage In Balb/C Mice: Biochemical And Histopathological Evaluation, Mustafa Cengi̇z, Adnan Ayhanci̇, Hati̇ce Mehtap Kutlu, Ahmet Musmul
Potential Therapeutic Effects Of Silymarin And Silymarin-Loaded Solid Lipidnanoparticles On Experimental Kidney Damage In Balb/C Mice: Biochemical And Histopathological Evaluation, Mustafa Cengi̇z, Adnan Ayhanci̇, Hati̇ce Mehtap Kutlu, Ahmet Musmul
Turkish Journal of Biology
Silymarin (Sm) is widely used in treating diseases that affect organs such as the liver, kidney, and gallbladder thanks to its antioxidative, renoprotective, antihepatotoxic, and anticarcinogenic properties. However, this substance is poorly solved in water and tends to decompose in the intestine, its bioavailability decreasing before it can show real effect. With these limitations in mind, the present study aims to enhance the poor bioavailability of Sm by forming Sm-loaded solid lipid nanoparticles (Sm-SLNs) using the hot homogenization method. A characterization process was undertaken to determine possible impact of Sm on experimental kidney damage. Our biochemical and light microscopic results …
Production Of 2,3-Butanediol By Enterobacter Cloacae From Corncob-Derived Xylose, Cuiying Zhang, Wei Li, Dongsheng Wang, Xuewu Guo, Lijuan Ma, Dongguang Xiao
Production Of 2,3-Butanediol By Enterobacter Cloacae From Corncob-Derived Xylose, Cuiying Zhang, Wei Li, Dongsheng Wang, Xuewu Guo, Lijuan Ma, Dongguang Xiao
Turkish Journal of Biology
During the process of industrial production of biofuels and biochemicals from lignocellulosic biomass, large amounts of waste byproducts rich in xylose are generated, resulting in excessive wastage of natural resources and environmental pollution. In this work, xylose solution from corncob hydrolysate was utilized to produce 2,3-butanediol (2,3-BD), using the strain Enterobacter cloacae CICC 10011 to improve the utilization rate of hemicellulose and reduce environmental pollution. 2,3-BD fermentation conditions were subsequently developed. Xylose solution and (NH4)2HPO4, selected with the Plackett-Burman experiment, were determined as significant independent variables to conduct a response surface experiment. With the optimized medium, 50.02 g/L 2,3-BD production …
Germline Transformation Of The Olive Fruit Fly, Bactrocera Oleae(Rossi) (Diptera: Tephritidae), With A Piggybac Transposon Vector, Hani̇fe Genç, Marc F. Schetelig, Xavier Nirmala, Alfred M. Handler
Germline Transformation Of The Olive Fruit Fly, Bactrocera Oleae(Rossi) (Diptera: Tephritidae), With A Piggybac Transposon Vector, Hani̇fe Genç, Marc F. Schetelig, Xavier Nirmala, Alfred M. Handler
Turkish Journal of Biology
The olive fruit fly, Bactrocera oleae, is a highly significant pest in olive growing countries, and controlling it may be enhanced by using genetically modified strains, especially for sterile insect technique programs. To improve and expand this technology, piggyBac-mediated germline transformation was achieved in a laboratory-adapted wild olive fruit fly strain. A piggyBac vector was used that is marked with both green (EGFP) and red (DsRed) fluorescent protein genes, with a duplicate piggyBac 5' terminal inverted repeat sequence inserted between the marker genes for subsequent immobilization of vectors integrated into the host genome. Five transformant G1 adults were selected based …
Involvement Of Amp-Activated Protein Kinase In The Protective Effectof Melatonin Against Renal Ischemia Reperfusion Injury, Kaouther Hadj Ayed Tka, Asma Mahfoudh Boussaid, Kaouther Kessabi, Rym Kammoun, Imed Messaoudi, Sonia Ghoul Mazgar, Joan Rosello Catafau, Hassen Ben Abdennebi
Involvement Of Amp-Activated Protein Kinase In The Protective Effectof Melatonin Against Renal Ischemia Reperfusion Injury, Kaouther Hadj Ayed Tka, Asma Mahfoudh Boussaid, Kaouther Kessabi, Rym Kammoun, Imed Messaoudi, Sonia Ghoul Mazgar, Joan Rosello Catafau, Hassen Ben Abdennebi
Turkish Journal of Biology
We investigated the implication of AMP-activated protein kinase (AMPK) in melatonin-induced protection in a renal ischemia/reperfusion (I/R) model. Animals were divided into four groups: rats of the sham group were not subjected to renal I/R. Rats in the I/R group received the vehicle 30 min before renal ischemia for 1 h followed by reperfusion for 6 h. Rats in the Mel+I/R group received melatonin (10 mg/kg) 30 min before the onset of I/R. Rats in the Mel+I/R+araA group received araA (AMPK inhibitor, 100 μg/kg per min for 10 min) just before melatonin administration. The results showed that melatonin treatment induced …
Identification, Characterization, And Expression Profiling Of Salt-Stress Tolerant Proton Gradient Regulator 5 (Pgr5) In Gossypium Arboreum, Muhammad Shahid, Adil Jamal, Beenish Aftab, Bahaeldeen Babiker Mohamed, Javed Wattoo, Muhammad Sarfraz Kiani, Bushra Rashid, Tayyab Husnain
Identification, Characterization, And Expression Profiling Of Salt-Stress Tolerant Proton Gradient Regulator 5 (Pgr5) In Gossypium Arboreum, Muhammad Shahid, Adil Jamal, Beenish Aftab, Bahaeldeen Babiker Mohamed, Javed Wattoo, Muhammad Sarfraz Kiani, Bushra Rashid, Tayyab Husnain
Turkish Journal of Biology
Salinity is among the significant abiotic stresses adversely affecting plant development and yield for a wide range of crops. For quantitative and qualitative expression studies of proton gradient regulator 5 (PGR5) under salt stress, differential display RT-PCR and rapid amplification of cDNA ends (RACE) were performed on salt-stress responsive Gossypium arboreum. Alignment of genomic and cDNA sequences revealed that the GPGR5 gene comprises a single open reading frame of 96 amino acids and contains no introns. Alignment of cotton GPGR5 complete amino acid sequence with PGR5 from other plants revealed the following identities: Gossypium raimondii (97%), Amaranthus hybridus (72%), Vitis …
Advancement In Protocol For In Vitro Seed Germination, Regeneration, Bulblet Maturation, And Acclimatization Of Fritillaria Persica, Derya Çakmak, Cuma Karaoğlu, Muhammad Aasim, Cengi̇z Sancak, Sebahatti̇n Özcan
Advancement In Protocol For In Vitro Seed Germination, Regeneration, Bulblet Maturation, And Acclimatization Of Fritillaria Persica, Derya Çakmak, Cuma Karaoğlu, Muhammad Aasim, Cengi̇z Sancak, Sebahatti̇n Özcan
Turkish Journal of Biology
The present study addressed the successful in vitro seed germination, bulblet regeneration, increased bulblet size, hardening, and acclimatization of Persian lily (Fritillaria persica). Seed germination rate was recorded as 86.7% and 96.7% after 2 and 3 months of cold treatment at 4 °C, respectively. Bulblet explants taken from a germination experiment were cultured on Murashige and Skoog (MS) medium supplemented with 0.5-2.0 mg/L thidiazuron (TDZ). Maximum bulblet regeneration frequency and bulblets per explants were achieved on a medium containing 2.0 mg/L TDZ. Leaf disc and leaf scale explants isolated from germinated seedlings were also cultured on MS medium containing 2-6 …
Short-Term Diabetes Decreases Ischemia Reperfusion-Induced Arrhythmia: The Effect Of Alpha-2 Blocker Yohimbine And Glibenclamide, Ömer Bozdoğan, Sali̇h Tunç Kaya, Selçuk Yaşar, Erçi̇n Çağdaş Baş, Talat Oğulcan Özarslan, Di̇dem Ekşi̇oğlu, Fi̇rdevs Eri̇m
Short-Term Diabetes Decreases Ischemia Reperfusion-Induced Arrhythmia: The Effect Of Alpha-2 Blocker Yohimbine And Glibenclamide, Ömer Bozdoğan, Sali̇h Tunç Kaya, Selçuk Yaşar, Erçi̇n Çağdaş Baş, Talat Oğulcan Özarslan, Di̇dem Ekşi̇oğlu, Fi̇rdevs Eri̇m
Turkish Journal of Biology
This study examined the effect of yohimbine alone and in combination with glibenclamide on ischemia and reperfusion-induced arrhythmias in diabetes. Six minutes of ischemia were produced in 1-week diabetic rats by ligation of the left coronary artery, and 6 min of reperfusion were produced by releasing the artery. A dose of 5 mg/kg of yohimbine and glibenclamide was administered for 7 days prior to the coronary ligation. The blood pressure, heart rate, and arrhythmia were determined from the recorded ECG during the 6 min of ischemia and reperfusion and then compared using a one-way ANOVA statistical program and the chi-square …
Sodium Borate Treatment Induces Metabolic Reprogramming In Hepatocellular Carcinoma Through Sirt3 Activation, Berna Üstüner, Hüseyi̇n Çi̇men
Sodium Borate Treatment Induces Metabolic Reprogramming In Hepatocellular Carcinoma Through Sirt3 Activation, Berna Üstüner, Hüseyi̇n Çi̇men
Turkish Journal of Biology
Sirtuins are NAD+-dependent deacetylases and ADP ribosyltransferases that are activated under stress conditions such as calorie restriction and starvation. Boron, which is mostly found in the form of boric acid (BA) or sodium borate (NaB), is known to bind NAD+. In this study, the effect of NaB on hepatoma cell line HEP3B was investigated by analyzing the proteins harvested from NaB-treated and serum-starved HEP3B cells. The NaB treatment (15 μg/mL) led to a decrease in the overall proteome acetylation and particularly in mitochondria, the synthesis rate of respiratory complexes, the amount of cellular reactive oxygen species, and the proliferation rate …
Prokaryotic Expression And Purification Of Porcine Sox6, Wanxue Wen, Xiaoling Chen, Zhiqing Huang, Meng Xu, Daiwen Chen, Bing Yu, Jun He, Junqiu Luo, Xiangbing Mao, Jie Yu, Ping Zheng, Hong Chen
Prokaryotic Expression And Purification Of Porcine Sox6, Wanxue Wen, Xiaoling Chen, Zhiqing Huang, Meng Xu, Daiwen Chen, Bing Yu, Jun He, Junqiu Luo, Xiangbing Mao, Jie Yu, Ping Zheng, Hong Chen
Turkish Journal of Biology
Sox6, a member of the Sox (Sry-related HMG box) family of transcription factors, plays an important role in embryonic muscle development and adult fast myofiber maintenance. Here we report the expression and purification of a His-tagged version of recombinant porcine Sox6 (pSox6) in Escherichia coli. The recombinant pSox6 was expressed as a C-terminal fusion protein with His6 tag in the E. Coli Rosetta (DE3) host strain. The protein was purified by Ni affinity chromatography, yielding approximately 5 μg/mL. The protein was identified by western blot analysis and confirmed by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) mass spectrometer analysis. In vitro biological …
Comparison Of Different Androgenesis Protocols For Doubled Haploid Plant Production In Ornamental Pepper (Capsicum Annuum L.), Esi̇n Ari, Tolga Yildirim, Nedi̇m Mutlu, Saadet Büyükalaca, Ümmü Gökmen, Ecehan Akman
Comparison Of Different Androgenesis Protocols For Doubled Haploid Plant Production In Ornamental Pepper (Capsicum Annuum L.), Esi̇n Ari, Tolga Yildirim, Nedi̇m Mutlu, Saadet Büyükalaca, Ümmü Gökmen, Ecehan Akman
Turkish Journal of Biology
Doubled haploid (DH) plant production plays an important role in pepper (Capsicum annuum L.) breeding for development of new varieties. However, information is lacking for DH plant production in pepper genotypes carrying ornamental value. The major goal of this study was to produce DH ornamental pepper lines having the mentioned value. Androgenic responses of 48 genotypes at the F2 or F3 generation were compared in each of three androgenesis protocols to determine the most effective method. Of the three protocols tested, anthers were placed on two different semisolid culture media and on a double-layer medium also called shed-microspore culture medium. …
Application Of Low Intensity Mechanical Vibrations For Bone Tissue Maintenance And Regeneration, Meli̇s Olçum Uzan, Öznur Baskan, Özge Karadaş, Engi̇n Özçi̇vi̇ci̇
Application Of Low Intensity Mechanical Vibrations For Bone Tissue Maintenance And Regeneration, Meli̇s Olçum Uzan, Öznur Baskan, Özge Karadaş, Engi̇n Özçi̇vi̇ci̇
Turkish Journal of Biology
Physical exercise is beneficial for bone tissue health, yet its usage is limited for preventing osteoporosis. Even though natural for the bone tissue from development to homeostasis, mechanical loads present with a multitude of physical parameters, including amplitude, duration, frequency, and distribution. Utilizing the most beneficial parameters of mechanical loads may potentiate a nonpharmaceutical tool for biotechnology to prevent and treat bone loss related to aging, bedrest, sedentary lifestyles, weightlessness, and other diseases. Low intensity vibrations (LIVs) consist of mechanical loads with amplitudes smaller than loads prescribed by habitual activity, with a higher frequency. In this review, literature covering LIV …
Cellular And Molecular Basis Of Cardiac Regeneration, Justin Judd, Wanling Xuan, Guo N. Huang
Cellular And Molecular Basis Of Cardiac Regeneration, Justin Judd, Wanling Xuan, Guo N. Huang
Turkish Journal of Biology
Heart disease is one of the largest causes of death in humans throughout the developed world. Despite its critical role in maintaining physiologic homeostasis, the adult mammalian heart does not exhibit a significant capacity for regeneration. By contrast, several lower vertebrates exhibit a remarkable ability to regenerate the aging myocardium in response to severe injury. Additionally, recent evidence has shed light on a transient regenerative window in postnatal mammals. In this review, we discuss key findings that help to unravel the differences in cardiac regeneration across phylogeny and ontogeny. Furthermore, we highlight recent significant progress in the pursuit of adult …
Regeneration And Healing Of Bone And Cartilage In Type-1 And Type-2 Diabetes: The Effects Of Insulin, Sümeyya Akyol, Mehmet Aki̇f Güleç, Hi̇lmi̇ Demi̇ri̇n, Ömer Akyol
Regeneration And Healing Of Bone And Cartilage In Type-1 And Type-2 Diabetes: The Effects Of Insulin, Sümeyya Akyol, Mehmet Aki̇f Güleç, Hi̇lmi̇ Demi̇ri̇n, Ömer Akyol
Turkish Journal of Biology
Increased fragility of long bones and delay in fracture healing in adults as well as skeletal development disorders in children are thought to be associated with endochondral ossification problems. The extracellular matrix (ECM) of cartilage is absorbed during endochondral ossification. During the recovery process from damage, chondrocytes undergo hypertrophy and increase their cell volume; on the other hand, it is also necessary to digest the ECM elements around the cells. Recent research on the proteolytic enzymes in ECM assumed to be responsible for the digestion is connected to proteinases digesting type II collagen and aggrecan. ADAMTS1, 4, and 5 are …
Underlying Mechanisms And Prospects Of Heart Regeneration, Gali̇p Servet Aslan, Dudu Gonca Misir, Fati̇h Kocabaş
Underlying Mechanisms And Prospects Of Heart Regeneration, Gali̇p Servet Aslan, Dudu Gonca Misir, Fati̇h Kocabaş
Turkish Journal of Biology
Findings in the last decade suggest that there is a considerable amount of cardiomyocyte turnover in the human heart throughout life, albeit not sufficient for heart regeneration following myocardial infarctions. Only a few species are known to be remarkably efficient in cardiac regeneration. They restore lost cardiomyocytes via a process of cardiomyocyte dedifferentiation, which is followed by robust proliferation of cardiomyocytes and incorporation into the myocardium. Similarly, neonatal mice have been recently shown to regenerate their heart following myocardial injuries. Studies with a neonatal cardiac regeneration mouse model suggest that the major source of new cardiomyocytes is likely to be …
Cardiomyogenic Differentiation Potential Of Human Lipoaspirate-Derivedstem Cells On Hyaluronic Acid/Gelatin Plasma Gels, Esra Göv, Hali̇me Kenar, Zehra Seda Halbutoğullari, Kazim Yalçin Arğa, Erdal Karaöz
Cardiomyogenic Differentiation Potential Of Human Lipoaspirate-Derivedstem Cells On Hyaluronic Acid/Gelatin Plasma Gels, Esra Göv, Hali̇me Kenar, Zehra Seda Halbutoğullari, Kazim Yalçin Arğa, Erdal Karaöz
Turkish Journal of Biology
Cardiomyogenic differentiation from mesenchymal stem cells has emerged as a novel approach for repair of damaged myocardium. Cell transplantation through direct cell injection is not an optimal method due to the lack of cell-extracellular matrix interactions. In the present study, differentiation potential of human adipose-derived stem cells (ASCs) to cardiomyocytes has been investigated by growing them on hyaluronic acid/gelatin (HA/G) plasma gels and coverslips and supplementing the growth medium with chemical modifiers (activin-a, BMP-4, insulin, valproic acid, and 5-azacytidine) in various combinations. The HA/G plasma gels were produced from human blood plasma-derived fibrinogen, gelatin, and human umbilical cord-derived hyaluronic acid. …
A Matter Of Regeneration And Repair: Caspases As The Key Molecules, Şehnaz Bolkent, Füsün Öztay, Selda Oktayoğlu, Serap Sancar Baş, Ayşe Karatuğ
A Matter Of Regeneration And Repair: Caspases As The Key Molecules, Şehnaz Bolkent, Füsün Öztay, Selda Oktayoğlu, Serap Sancar Baş, Ayşe Karatuğ
Turkish Journal of Biology
Researchers have been focused on understanding the pathogenesis of human diseases. They have been working to find the roles of caspases in the balance between apoptosis, autophagy, pyroptosis, and necroptosis, and also in the regeneration of damaged tissue. At this point, besides their death-inducing roles, new findings indicate the role of caspases in proliferation for maintaining the viability of cells in response to signals from apoptotic cells. Recently, determining cell fate has also been identified among other functions of caspases. Findings indicate that caspases direct cellular pathways to cell differentiation by suppressing stem cell self-renewal. The common opinion about the …
Collagen/Peo/Gold Nanofibrous Matrices For Skin Tissue Engineering, Ömer Aktürk, Di̇lek Keski̇n
Collagen/Peo/Gold Nanofibrous Matrices For Skin Tissue Engineering, Ömer Aktürk, Di̇lek Keski̇n
Turkish Journal of Biology
As a novel approach in skin tissue engineering, gold nanoparticles (AuNPs) were synthesized and incorporated at different concentrations into collagen/PEO nanofibrous matrices in this study. The group containing 14.27 ppm AuNPs (CM-Au) had the best nanofibrous morphology. CM-Au was cross-linked with glutaraldehyde vapor (CM-AuX). All groups were disrupted in collagenase in 2 h, but cross-linked groups and Matriderm1397904493 resisted hydrolytic degradation for 7 and 14 days, respectively. Due to its small pores and dense structure, lower water swelling results (7.26 ± 2.62 g/g) were obtained for CM-AuX than Matriderm (17.51 ± 1.97 g/g). CM-Au and Matriderm had statistically similar tensile …
Triterpenoid Saponin-Rich Fraction Of Centella Asiatica Decreases Il-1ss Andnf-?B, And Augments Tissue Regeneration And Excision Wound Repair, Amena Mahmood, Ambrish Tiwari, Kazi̇m Şahi̇n, Ömer Küçük, Shakir Ali
Triterpenoid Saponin-Rich Fraction Of Centella Asiatica Decreases Il-1ss Andnf-?B, And Augments Tissue Regeneration And Excision Wound Repair, Amena Mahmood, Ambrish Tiwari, Kazi̇m Şahi̇n, Ömer Küçük, Shakir Ali
Turkish Journal of Biology
Wound healing is an orderly process driven by numerous cellular mediators that aim to accelerate it. In skin conditions such as leprosy and psoriasis, Centella asiatica, which contains high amounts of triterpenoid saponins (TSs), has been reported to have beneficial effects. This study examined the effects of TS fraction of the herb on tissue regeneration and excision wound repair in rat skin. Briefly, TS fraction was prepared from C. Asiatica and applied on the wound area for 7 consecutive days. The IL-1β, NF-κB, and other biochemical parameters were then estimated, followed by examination of inflammatory cell infiltration, epithelialization, angiogenesis, and …
The Sca-1+ Mesenchymal Stromal Cells Modulate Macrophage Commitment And Function, Yaozhen Chen, Yan Chen, Dandan Yin, Yazhou Wang, Zheng Liu, Ning An, Fan Wen, Na Li, Jiajia Xin, Xingbin Hu, Huijie Zhang, Wen Yin
The Sca-1+ Mesenchymal Stromal Cells Modulate Macrophage Commitment And Function, Yaozhen Chen, Yan Chen, Dandan Yin, Yazhou Wang, Zheng Liu, Ning An, Fan Wen, Na Li, Jiajia Xin, Xingbin Hu, Huijie Zhang, Wen Yin
Turkish Journal of Biology
Mesenchymal stromal cells (MSCs) have been characterized as an important component of the hematopoietic niche that plays a role in regulating hematopoietic stem cell (HSC) quiescence, self-renewal, and lineage commitment. Macrophages, one kind of innate immune cells, are mainly developed from HSCs in niche. However, how MSCs and their subpopulations regulate macrophage commitment is still unknown. The current study compared the contribution of MSCs and Sca-1+MSCs to macrophage commitment and further modulation on the function of macrophages. We found that Sca-1+MSCs could promote macrophage commitment through the M-CSFR gene, and Sca-1+MSCs led macrophage polarization towards the M2 phenotype. Further functional …
Role Of Melatonin On Differentiation Of Mesenchymal Stem Cells Derived From Third Molar Germ Tissue, Nurullah Aydoğdu, Paki̇ze Nesli̇han Taşli, Hati̇ce Burcu Şi̇şli̇, Mehmet Emi̇r Yalvaç, Fi̇kretti̇n Şahi̇n
Role Of Melatonin On Differentiation Of Mesenchymal Stem Cells Derived From Third Molar Germ Tissue, Nurullah Aydoğdu, Paki̇ze Nesli̇han Taşli, Hati̇ce Burcu Şi̇şli̇, Mehmet Emi̇r Yalvaç, Fi̇kretti̇n Şahi̇n
Turkish Journal of Biology
Stem cell-based applications have become a popular and promising approach for therapy for a number of disorders including neurodegenerative diseases, degenerative muscle diseases, and osteoporosis, as well as trauma, inflammations, burns, and injuries. Human tooth germ stem cells are an adult stem cell source; they have mesenchymal stem cell properties and show high proliferative and differentiation capacity. Melatonin has been demonstrated to regulate differentiation of human and mouse mesenchymal stem cells into various cell lineages in addition to its other functions in the body. In the current study, the effects of melatonin on osteogenic, neurogenic, adipogenic, chondrogenic, myogenic, and odontogenic …
The Restorative Effect Of Ascorbic Acid On Liver Injury Inducedby Asymmetric Dimethylarginine, Ali̇ Okuyucu, Osman Şaliş, Ömer Alici, Abdullah Güvenli̇, Yüksel Terzi̇, Muhammed Emi̇n Keleş, Fati̇h İlkaya, İbrahi̇m Gören, Hasan Alaçam
The Restorative Effect Of Ascorbic Acid On Liver Injury Inducedby Asymmetric Dimethylarginine, Ali̇ Okuyucu, Osman Şaliş, Ömer Alici, Abdullah Güvenli̇, Yüksel Terzi̇, Muhammed Emi̇n Keleş, Fati̇h İlkaya, İbrahi̇m Gören, Hasan Alaçam
Turkish Journal of Biology
We aimed to determine whether increased levels of asymmetric dimethylarginine (ADMA) directly cause hepatic deterioration and whether it is decreased by ascorbic acid (AA) as an antioxidant. The study included three groups: Control (n = 10), ADMA (n = 10), and ADMA + AA (n = 10). ADMA was administered at 2 mg kg-1 day-1 and AA was given at 50 mg kg-1 day-1 for 10 days. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase, and alkaline phosphatase activities were analyzed. Histomorphological evaluation of the liver was performed based on the Ishak scoring system. When compared with the control, …
Construction Of A Tissue-Engineered Human Corneal Endothelium And Its Transplantation In Rabbit Models, Jun Zhao, Xiya Ma, Tingjun Fan
Construction Of A Tissue-Engineered Human Corneal Endothelium And Its Transplantation In Rabbit Models, Jun Zhao, Xiya Ma, Tingjun Fan
Turkish Journal of Biology
Human corneal endothelium (HCE) is vital in maintaining corneal transparency and thickness, and damages to it may result in endotheliopathy and even corneal blindness, which can only be cured by keratoplasty. Due to the shortage of donor corneas, tissue-engineered HCE (TE-HCE) has become an equivalent to HCE. This study constructs a high endothelial cell density (ECD) TE-HCE and evaluates its functions by corneal transplantation in rabbits. A TE-HCE was constructed by culturing DiI-labeled nontransfected HCE cells on modified denuded amniotic membrane (mdAM) using collagen IV, fibronectin, and laminin, and rabbit corneas were characterized both in vivo and ex vivo after …
Evaluation Of Biocompatibility Of Random Or Aligned Electrospun Polyhydroxybutyrate Scaffolds Combined With Human Mesenchymal Stem Cells, Sevi̇l Köse, Fati̇ma Aerts Kaya, Emi̇r Baki̇ Denkbaş, Petek Korkusuz, Fahri̇ye Duygu Çeti̇nkaya
Evaluation Of Biocompatibility Of Random Or Aligned Electrospun Polyhydroxybutyrate Scaffolds Combined With Human Mesenchymal Stem Cells, Sevi̇l Köse, Fati̇ma Aerts Kaya, Emi̇r Baki̇ Denkbaş, Petek Korkusuz, Fahri̇ye Duygu Çeti̇nkaya
Turkish Journal of Biology
Polyhydroxybutyrate (PHB) is a polymer used to restore tissues or regenerate bones. However, its compatibility with human bone marrow-derived mesenchymal stem cells (MSCs) has not been examined. PHB membranes of random (r-PHB) or aligned (a-PHB) electrospun nanofibers that generate bone and spinal axon scaffolds were combined with human MSCs. The adhesion and proliferation of cells on these membranes were examined. The orientation of cells on PHB membranes was analyzed by confocal microscopy and scanning electron microscopy (SEM). The MSCs maintained their characteristic properties on the membranes, adhered to the membranes, and preserved their viability. The cell morphology was different when …
Third-Party Wharton?S Jelly Mesenchymal Stem Cells For Treatment Of Steroid-Resistant Acute And Chronic Graft-Versus-Host Disease: A Report Of 10 Cases, Dariusz Boruczkowski, Dominika Gladysz, Slawomir Ruminski, Iwona Czaplicka-Szmaus, Magdalena Murzyn, Artur Olkowicz, Krzysztof Kalwak, Monika Mielcarek, Katarzyna Drabko, Jan Styczynski, Miroslaw Markiewicz, Katarzyna Pawelec, Maciej Boruczkowski, Tomasz Oldak
Third-Party Wharton?S Jelly Mesenchymal Stem Cells For Treatment Of Steroid-Resistant Acute And Chronic Graft-Versus-Host Disease: A Report Of 10 Cases, Dariusz Boruczkowski, Dominika Gladysz, Slawomir Ruminski, Iwona Czaplicka-Szmaus, Magdalena Murzyn, Artur Olkowicz, Krzysztof Kalwak, Monika Mielcarek, Katarzyna Drabko, Jan Styczynski, Miroslaw Markiewicz, Katarzyna Pawelec, Maciej Boruczkowski, Tomasz Oldak
Turkish Journal of Biology
Severe graft-versus-host disease (GvHD) is a life-threatening complication after allogeneic hematopoietic stem cell transplantation (HSCT). Bone marrow mesenchymal stem cells (BM-MSCs) display immunosuppressive activity and have been previously shown to improve the outcome of GvHD therapy. We describe the use of umbilical cord mesenchymal stem cells (WJ-MSCs) expanded in serum-free medium for the treatment of acute GvHD (aGvHD) and chronic GvHD (cGvHD) in 10 patients. After 1 to 3 WJ-MSC infusions at a median dose of 1.5 × 106 cells/kg recipient body weight, a complete or partial response was observed in 4/7 patients with aGvHD and in 2/3 patients with …
Osteogenic Differentiation Of Electrostimulated Human Mesenchymal Stem Cells Seeded On Silk-Fibroin Films, Anil Sera Çakmak, Soner Çakmak, James White, Waseem Raja, David Kaplan, Menemşe Gümüşdereli̇oğlu
Osteogenic Differentiation Of Electrostimulated Human Mesenchymal Stem Cells Seeded On Silk-Fibroin Films, Anil Sera Çakmak, Soner Çakmak, James White, Waseem Raja, David Kaplan, Menemşe Gümüşdereli̇oğlu
Turkish Journal of Biology
Electric field is known as an important regulator to guide the development and regeneration of many tissues. The aim of this study was to investigate the osteogenic differentiation potential of human mesenchymal stem cells (hMSCs) cultivated on silk-fibroin films in response to different parameters, i.e. Frequency, voltage, distance between electrodes, and/or culture conditions (growth medium or osteogenic medium). Silk films were prepared in the presence of platinum wires to study the impact of exogenous electrostimulation on the cells for up to 14 days. The experimental groups can be defined as high voltage in osteogenic differentiation medium, low voltage in osteogenic …