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Full-Text Articles in Entire DC Network
Identification Of The Cysteine-Rich 61 (Cyr61) Gene Variations In Osteosarcoma Patients, Syed Rizwan Hussain, Sabir Ali, Ajai Singh, Vineet Kumar, Nazia Rizivi, Manish Yadav, Mohammad Kaleem Ahmad, Vijay Kumar, Abbas Ali Mahdi
Identification Of The Cysteine-Rich 61 (Cyr61) Gene Variations In Osteosarcoma Patients, Syed Rizwan Hussain, Sabir Ali, Ajai Singh, Vineet Kumar, Nazia Rizivi, Manish Yadav, Mohammad Kaleem Ahmad, Vijay Kumar, Abbas Ali Mahdi
Turkish Journal of Medical Sciences
Background/aim: Osteosarcoma requires an angiogenesis process. CYR61 is one of the extracellular signaling molecules that promote angiogenesis, tumor growth, and the malignancy of osteosarcoma. In the present study, we investigate the CYR61 gene variations in osteosarcoma and their correlations with clinicopathological findings. Materials and methods: We performed variation analysis of the CYR61 gene in 58 patients with osteosarcoma. With an aim to ascertain the variety of variations in exons 2, 3, 4, and 5 of the CYR61 gene in osteosarcoma, we did a PCR-SSCP followed by DNA sequencing. Results: In osteosarcoma the CYR61 gene variations found were 18.96% (11/58) in …
A New Angiogenesis Prognostic Index With Vegfa, Plgf, And Angiopoietin1 Predicts Survival In Patients With Advanced Gastric Cancer, Sedef Hande Aktaş, Hakan Akbulut, Ozan Yazici, Nurullah Zengi̇n, Hali̇me Nalan Akgün, Zeki̇ Üstüner, Fi̇kri̇ İçli̇
A New Angiogenesis Prognostic Index With Vegfa, Plgf, And Angiopoietin1 Predicts Survival In Patients With Advanced Gastric Cancer, Sedef Hande Aktaş, Hakan Akbulut, Ozan Yazici, Nurullah Zengi̇n, Hali̇me Nalan Akgün, Zeki̇ Üstüner, Fi̇kri̇ İçli̇
Turkish Journal of Medical Sciences
Background/aim: The role of angiogenic factors in gastric cancer is not clear. We aimed to assess the role of vascular endothelial growth factor A (VEGFA), angiopoietin 1 (Ang-1), and placental growth factor (PlGF) in the prognosis of patients with advanced gastric cancer. Materials and methods: Thirty consecutive patients treated with a modified DCF (docetaxel, cisplatin, and fluorouracil) regimen were included in the study. The plasma VEGFA, Ang-1, and PlGF levels of the patients before treatment and following two cycles of chemotherapy were measured and evaluated as prognostic factors. Results: Poor performance status and lower Ang-1 levels were correlated with poor …
Relationship Between Selected Micronutrient Deficiencies And Oxidative Stress Biomarkers In Diabetes Mellitus Patients With Foot Ulcers In Ibadan, Nigeria, Elizabeth Bosede Bolajoko, Olubayo Michael Akinosun, John Anetor, Kensese Sontin Mossanda
Relationship Between Selected Micronutrient Deficiencies And Oxidative Stress Biomarkers In Diabetes Mellitus Patients With Foot Ulcers In Ibadan, Nigeria, Elizabeth Bosede Bolajoko, Olubayo Michael Akinosun, John Anetor, Kensese Sontin Mossanda
Turkish Journal of Medical Sciences
Background/aim: Micronutrients are indispensable in the prevention of diseases and maintenance of good health. Their deficiencies have been implicated in several diseases associated with oxidative stress (OS). This study aimed at investigating the levels of some vitamins and minerals in association with OS markers in diabetic foot ulcer (DFU) patients in Ibadan, Oyo State, Nigeria. Materials and methods: Seventy DFU patients and 50 apparently healthy volunteers (controls) were recruited for the study. Blood samples of 10 mL were collected after a 10-h overnight fast from each participant after obtaining their consent. Levels of oxidative stress biomarkers such as lipid peroxide …
The Clinical Features And Outcomes Of Turkish Patients With Igg4-Related Disease:A Single-Center Experience, Ömer Karadağ, Abdulsamet Erden, Emi̇ne Arzu Ayhan, Ertuğrul Çağri Bölek, Umut Kalyoncu, Berkan Armağan, Alper Sari, Levent Kiliç, Ali̇ Akdoğan, Tuncay Hazirolan, Bülent Akdoğan, Şazi̇ye Şule Apraş Bi̇lgen, Di̇lek Baydar, Sedat Ki̇raz, Ali̇ İhsan Ertenli̇
The Clinical Features And Outcomes Of Turkish Patients With Igg4-Related Disease:A Single-Center Experience, Ömer Karadağ, Abdulsamet Erden, Emi̇ne Arzu Ayhan, Ertuğrul Çağri Bölek, Umut Kalyoncu, Berkan Armağan, Alper Sari, Levent Kiliç, Ali̇ Akdoğan, Tuncay Hazirolan, Bülent Akdoğan, Şazi̇ye Şule Apraş Bi̇lgen, Di̇lek Baydar, Sedat Ki̇raz, Ali̇ İhsan Ertenli̇
Turkish Journal of Medical Sciences
Background/aim: Since the majority of the IgG4-related disease (IgG4-RD) patients in the literature are from the Far East and the United States, there is a lack of large series from other parts of the world. We aimed to identify the clinical characteristics and outcome of Turkish IgG4-RD patients from a tertiary center.Materials and methods: Fifty-two patients classified as having definite IgG4-RD according to comprehensive diagnostic criteria were included in the study. Patients not fulfilling the definite criteria due to lack of pathologic specimen and/or serum IgG4 levels were excluded (n = 47). Clinical, laboratory, and histopathological features and treatment approaches …
The Relationship Between Nonsuicidal Self-Injurious Behavior And Manners Of Death: A Multicenter Autopsy Study, Sai̇t Özsoy, Hacer Yaşar Teke, Hüseyi̇n Çeti̇n Ketenci̇, Ertuğrul Gök, Kenan Karbeyaz, Taner Akar, Hani̇fe Alkurt-Alkan, Mesut Akyol, Mehmet Ayhan Cöngöloğlu, Sermet Sezi̇gen, Ahmet Zahi̇t Dursun, Alper Özkök, Serhat Sari
The Relationship Between Nonsuicidal Self-Injurious Behavior And Manners Of Death: A Multicenter Autopsy Study, Sai̇t Özsoy, Hacer Yaşar Teke, Hüseyi̇n Çeti̇n Ketenci̇, Ertuğrul Gök, Kenan Karbeyaz, Taner Akar, Hani̇fe Alkurt-Alkan, Mesut Akyol, Mehmet Ayhan Cöngöloğlu, Sermet Sezi̇gen, Ahmet Zahi̇t Dursun, Alper Özkök, Serhat Sari
Turkish Journal of Medical Sciences
Background/aim: This study aimed to determine the relationship between nonsuicidal self-injurious behavior (NSSIB) and manners of death.Materials and methods: This study retrospectively evaluated 6604 autopsy cases evaluated at forensic medicine institutes of six major cities of Turkey. The study group consisted of all cases with NSSIB findings. The control group was created from cases without signs of NSSIB. Results: We found that the numbers of possible suicide and homicide cases in the NSSIB (+) group were significantly higher than in the NSSIB (-) group. The possibility of death due to suicide was 3.213 times and homicide was 2.004 times higher …
Investigation Of Effects Of Fucoidan Polysaccharides Extracted From Twospecies Of Padina On The Wound-Healing Process In The Rat, Moazameh Kordjazi, Bahareh Shabanpour, Ebrahim Zabihi, Mohammad Ali Faramarzi, Hassan Ahmadi Gavlighi, Seyed Mohammad Amin Feghhi, Seyed Abbas Hosseini
Investigation Of Effects Of Fucoidan Polysaccharides Extracted From Twospecies Of Padina On The Wound-Healing Process In The Rat, Moazameh Kordjazi, Bahareh Shabanpour, Ebrahim Zabihi, Mohammad Ali Faramarzi, Hassan Ahmadi Gavlighi, Seyed Mohammad Amin Feghhi, Seyed Abbas Hosseini
Turkish Journal of Veterinary & Animal Sciences
Seaweeds contains different types of polysaccharides with high abilities. The aim of this study was to determine the potential of fucoidan polysaccharides for wound-healing properties. Fucoidans of two species of Padina (P. tetrastromatica and P. boergesenii), collected from the Persian Gulf northern coastal area, were extracted and analyzed using high-performance anion-exchange chromatography. Purified fucoidans were used to prepare a 2% topical ointment. Under aseptic conditions, two identical paravertebral full-thickness skin wounds (either by burn or excision) were made in 40 anesthetized male rats allocated into 4 groups. Either fucoidan ointments or the vehicle (ointment base) were applied to rats' wounds …
Effects Of Season On Semen Parameters And Relationships Between Selected Semen Characteristics In Hypor Boars, Krzysztof Górski, Stanislaw Kondracki, Anna Wysokinska
Effects Of Season On Semen Parameters And Relationships Between Selected Semen Characteristics In Hypor Boars, Krzysztof Górski, Stanislaw Kondracki, Anna Wysokinska
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to evaluate the effect of the season of the year on the quality of Hypor boar semen and to determine the correlations between selected semen characteristics in particular seasons. Ejaculates from Hypor boars were analyzed for volume, sperm concentration, percentage of spermatozoa with normal motility, total number of spermatozoa, and the number of insemination doses per ejaculate. Microscopic slides were prepared from semen samples. Each sample was evaluated for the morphology of spermatozoa. Spermatozoa with normal morphology were counted, as well as those with both major and minor abnormalities. Spermatozoa were measured for morphometric …
Extraskeletal Chondroblastic Osteosarcoma Of The Subcutaneous Tissue In A Maltese Dog, Byungjoon Seung, Junghyung Ju, Seunghee Cho, Soohyeon Kim, Hwan Choi, Junghyang Sur
Extraskeletal Chondroblastic Osteosarcoma Of The Subcutaneous Tissue In A Maltese Dog, Byungjoon Seung, Junghyung Ju, Seunghee Cho, Soohyeon Kim, Hwan Choi, Junghyang Sur
Turkish Journal of Veterinary & Animal Sciences
A 10-year-old spayed female Maltese dog presented to a local animal hospital with a subcutaneous mass (4 x 3 x 3 cm) in the right shoulder region. The mass was well circumscribed, with soft tissue opacity and variable levels of mineralization, but with no bony involvement in radiography. The mass was surgically removed. Upon histological examination, the mass consisted of malignant mesenchymal cells that had produced a chondroid matrix and osteoid. Round to polygonal neoplastic cells with large nuclei showed moderate anisokaryosis and variable numbers of mitotic figures. The tumor cells were positive for vimentin and osteocalcin and negative for …
Downregulation Of Cystathionine γ Lyase And Endothelial Nitric Oxide Synthase And Reduced Responsiveness Of α1a Adrenergic Receptors In The Kidneys Of Left Ventricular Hypertrophied Wistar Kyoto Rats, Ashfaq Ahmad, Munavvar Abdul Sattar, Hassaan Anwer Rathore, Safia Akhtar Khan, Nor Azizan Abdullah, Edward James Johns
Downregulation Of Cystathionine γ Lyase And Endothelial Nitric Oxide Synthase And Reduced Responsiveness Of α1a Adrenergic Receptors In The Kidneys Of Left Ventricular Hypertrophied Wistar Kyoto Rats, Ashfaq Ahmad, Munavvar Abdul Sattar, Hassaan Anwer Rathore, Safia Akhtar Khan, Nor Azizan Abdullah, Edward James Johns
Turkish Journal of Biology
This article explores the relationship between the renal expression of cystathionine gamma lyase (CSE) and endothelial nitric oxide synthase (eNOS) and the responsiveness of α1A adrenergic receptors in the renal vasculature following left ventricular hypertrophy (LVH) in rats. LVH was established by administering isoprenaline (5 mg/kg, 5 injections subcutaneously, 72 h apart) with 62 mg/L caffeine in drinking water for 2 weeks. Renal vasoconstrictor responses were measured using local administration of adrenergic agonists noradrenaline (NA), phenylephrine (PE), and methoxamine (ME) and the selective α1A adrenergic antagonist 5-methylurapidil (5-MeU). Mean arterial blood pressure was higher (144 ± 9 vs. 116 ± …
Cloning And Abiotic Stress Resistance Analyses Of A New Proline-Glycine-Alaninehistidine-Rich Protein Gene From Ipomoea Batatas (L.) Lam., Xiang Tao, Bin Yong, Huan-Huan Shao, Xin-Rong Ma
Cloning And Abiotic Stress Resistance Analyses Of A New Proline-Glycine-Alaninehistidine-Rich Protein Gene From Ipomoea Batatas (L.) Lam., Xiang Tao, Bin Yong, Huan-Huan Shao, Xin-Rong Ma
Turkish Journal of Biology
Sweet potato [Ipomoea batatas (L.) Lam.] is one of the top six most important crops in the world in terms of production. It has the capacity to tolerate marginal growing conditions better than most other crops. In this study, a proline- and glycine-rich protein encoding gene, IbPGAHRP1, was isolated and characterized from the transcriptome of sweet potato cultivar Xushu 18. Sequence analyses showed that proline, glycine, alanine, and histidine account for 62.6% of the total amino acid residues. Two proline-rich tandem repeats, an alanine-rich repeat, a histidine-rich tandem repeat, and five XYPP motifs were present in IbPGAHRP1. Sequence similarity analysis …
Effect Of Polyamines On Somatic Embryogenesis Via Mature Embryo In Wheat, Murat Aydin, Arash Hossein Pour, Kami̇l Hali̇loğlu, Meti̇n Tosun
Effect Of Polyamines On Somatic Embryogenesis Via Mature Embryo In Wheat, Murat Aydin, Arash Hossein Pour, Kami̇l Hali̇loğlu, Meti̇n Tosun
Turkish Journal of Biology
Plant tissue culture via somatic embryogenesis plays a key role in wheat genetic transformation studies. Therefore, the production of embryogenic calli with a high regeneration capacity is a prerequisite for efficient plant regeneration. Although immature embryos are the best type of explants for plant regeneration via somatic embryogenesis, the use of mature embryos has remarkable advantages compared to immature embryos as explants. However, the regeneration capacity of mature embryos is still lower than that of immature embryos. Plant regeneration from somatic embryos can only occur when they are mature enough. Moreover, the maturation of somatic embryos and their conversion into …
High Levels Of Polyphosphate Kinase Affect Not Only Endotoxin Production But Also Acid Tolerance Of Bacillus Thuringiensis, Tuğrul Doruk, Zeynep Gi̇rgi̇n Ersoy, Mehmet Sali̇m Öncel, Sedef Tunca Gedi̇k
High Levels Of Polyphosphate Kinase Affect Not Only Endotoxin Production But Also Acid Tolerance Of Bacillus Thuringiensis, Tuğrul Doruk, Zeynep Gi̇rgi̇n Ersoy, Mehmet Sali̇m Öncel, Sedef Tunca Gedi̇k
Turkish Journal of Biology
Inorganic phosphate is stored as a polyphosphate (polyP) polymer inside every living cell. This polymer is synthesized by the polyP kinase (PPK) enzyme using the terminal phosphate of ATP as substrate and it performs important functions in the cell. In this study, effects of high levels of PPK on Bacillus thuringiensis subsp. israelensis were analyzed. Recombinant Bti ppk, a PPK overproducer, was found to uptake more phosphate into the cell and produce a consistently higher amount of endotoxin than the wild type under culture conditions including a range of temperatures (25 °C, 30 °C, and 37 °C), pH values (pH …
Copper Enriches Efficacy Of Dp44mt In Breast Cancer Cells, Ufuk Özer
Copper Enriches Efficacy Of Dp44mt In Breast Cancer Cells, Ufuk Özer
Turkish Journal of Biology
Neoplastic cells need essential metals, such as iron and copper, for cellular functions and rapid growth. Metal chelation and changes in their redox cycle in favor of oxidative stress may be critical for making these cells vulnerable to cell death. The HER2-overexpressing breast cancer cell line, MCF7-HER2, and its vehicle control, MCF7-vec cells, were treated with CuCl2, FeSO4, and Dp44mT. Reactive oxygen species (ROS) production in response to these chemicals was detected with flow cytometry, and cell viability was measured by MTT assay in the cells. ROS levels were relatively increased by Dp44mT in the cells, and this was reversed …
Identification And Cloning Of Highly Epitopic Regions Of Clostridium Novyi Alpha Toxin, Najme Gord Noshahri, Mohsen Fathi Najafi, Ali Makhdoumi Kakhki, Behjat Majidi, Mohsen Mehrvarz
Identification And Cloning Of Highly Epitopic Regions Of Clostridium Novyi Alpha Toxin, Najme Gord Noshahri, Mohsen Fathi Najafi, Ali Makhdoumi Kakhki, Behjat Majidi, Mohsen Mehrvarz
Turkish Journal of Biology
The aim of the present study is to provide a strategy for predicting the production of antigenic regions of Clostridium novyi alpha toxin. The selection is based on B-cell epitopes and MHCII binding protein by immunoinformatics tools. The study resulted in identifying antigenic regions in the beginning and middle (3-17 and 965-997 amino acid residues) as suitable binders to MHCII and the carboxyl terminal of the protein (1800-1958 amino acid residues) in B-cell epitopes. The appropriate region in B-cell epitopes was chosen for cloning. The presence of recombinant protein was detected with immunological methods. Subsequently, BALB/C mice were immunized with …
Plantlet Regeneration From Young Leaf Segments Of Curaua (Ananas Erectifolius), An Amazon Species, Carolina Moreira, Helena Andrade, Suzan Bertolucci, Osmar Lameira, Aliyu Mohammed, José Eduardo Pinto
Plantlet Regeneration From Young Leaf Segments Of Curaua (Ananas Erectifolius), An Amazon Species, Carolina Moreira, Helena Andrade, Suzan Bertolucci, Osmar Lameira, Aliyu Mohammed, José Eduardo Pinto
Turkish Journal of Biology
The present study reports the first efficient in vitro regeneration of Ananas erectifolius via indirect organogenesis. Leaf segments (leaf base, middle, and apex) excised from 3- or 5-week-old in vitro plantlets were cultured on 1/4 strength MS medium supplemented with different concentrations and combinations of plant growth regulators. The explants were also exposed to pulse treatment with thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) for different periods of time. The results showed that using juvenile rather than old explants enhanced the frequency of callus induction (35.0% and 16.0%, respectively). Among the explant types tested, only leaf base segments induced calli; the …
High Dietary Fructose Load Aggravates Lipid Metabolism In The Liver Of Wistar Rats Through Imbalance Between Lipogenesis And Fatty Acid Oxidation, Ana Teofilovic, Biljana Bursac, Ana Djordjevic, Danijela Vojnovic Milutinovic, Gordana Matic, Natasa Velickovic
High Dietary Fructose Load Aggravates Lipid Metabolism In The Liver Of Wistar Rats Through Imbalance Between Lipogenesis And Fatty Acid Oxidation, Ana Teofilovic, Biljana Bursac, Ana Djordjevic, Danijela Vojnovic Milutinovic, Gordana Matic, Natasa Velickovic
Turkish Journal of Biology
Fructose ingestion is often associated with hepatic steatosis and hypertriglyceridemia. The homeostasis of hepatic lipids is mainly determined by the interplay of lipogenesis and fatty acid β-oxidation. In this study, we hypothesized that high fructose intake disturbs hepatic lipid metabolism through an imbalance between these processes. Therefore, we analyzed the effects of a 9-week-long consumption of a 60% fructose solution on physiological parameters, glycemia, and blood lipid profiles in male Wistar rats. The expression of key regulators of fatty acid oxidation (FAO) and lipogenesis in the liver were assessed by western blot and quantitative polymerase chain reaction. The results showed …
Preparation And Characterization Of Acellular Porcinepericardium For Cardiovascular Surgery, Ha Le Bao Tran, Trang Thi Huyen Dinh, My Thi Ngoc Nguyen, Quan Minh To, Anh Tho Tuan Pham
Preparation And Characterization Of Acellular Porcinepericardium For Cardiovascular Surgery, Ha Le Bao Tran, Trang Thi Huyen Dinh, My Thi Ngoc Nguyen, Quan Minh To, Anh Tho Tuan Pham
Turkish Journal of Biology
The aim of this study was to fabricate and characterize acellular porcine pericardium that can be used to patch cardiovascular repair. Porcine pericardial tissues were treated with 10 mM Tris-HCl and sodium dodecyl sulfate (SDS) at different concentrations and time intervals. The optimal decellularization protocol was determined according to elimination of cellular components and DNA via histology and DNA quantification, respectively. There were no significant changes in stress and fracture strain after decellularization. Liquid extracts from the decellularized pericardium caused no cytotoxicity towards human fibroblasts, were capable of supporting an appropriate attachment of human endothelial progenitor cells, and did not …
Cytotoxic Effects Of Peanut Phenolic Compounds Possessing Histone Deacetylase Inhibitory Activity On Human Colon Cancer Cell Lines, Somprasong Saenglee, Sanun Jogloy, A. Patanothai, Thanaset Senawong
Cytotoxic Effects Of Peanut Phenolic Compounds Possessing Histone Deacetylase Inhibitory Activity On Human Colon Cancer Cell Lines, Somprasong Saenglee, Sanun Jogloy, A. Patanothai, Thanaset Senawong
Turkish Journal of Biology
Phenolic compounds present in our diet play an important role in colon cancer chemoprevention. Previous results demonstrated that peanut testa extract inhibited both histone deacetylase (HDAC) activity and the growth of colon cancer cells. In this study, four identified phenolic compounds in peanut testae (resveratrol, p-coumaric acid, ferulic acid, and sinapinic acid) were investigated for their HDAC inhibitory and anticancer activities against colon cancer cell lines. In vitro study revealed that resveratrol exhibited the greatest HDAC inhibitory activity. Molecular docking studies demonstrated that all four compounds could bind both HDAC1 and HDAC2. Resveratrol exhibited the most effective antiproliferative activity against …
The Sensitivity Of 5-Formylcytosine To Doxorubicin Regardless Of Dna Damage, Selcen Çeli̇k Uzuner, Chris O'Neill
The Sensitivity Of 5-Formylcytosine To Doxorubicin Regardless Of Dna Damage, Selcen Çeli̇k Uzuner, Chris O'Neill
Turkish Journal of Biology
We have previously shown that DNA damage induced by doxorubicin was associated with an increase in the level of DNA methylation, 5meC, in mouse embryonic fibroblasts using a developed immunostaining methodology. Doxorubicin (also known as adriamycin), which induces double-strand DNA breaks, has been widely used in cancer therapy. Cytosine modifications are dynamically regulated in the genome during lifespan, but abnormal changes in the 5meC pattern can be associated with diseases in particular cancer progressions and DNA damage. One of the side effects of cancer therapy is that chemotherapeutic agents can damage healthy cells as well as cancerous cells. In this …
Selective Suppression Of Tumor Cells By A Tumor-Specific Bicistronic Lentiviral Vector, Saeed Ranjbar, Mohammad Sadegh Hashemzadeh, Shahryar Khoshtinat Nikkhoi, Alireza Farasat, Mahdi Tat, Majdedin Ghalavand, Ruhollah Dorostkar
Selective Suppression Of Tumor Cells By A Tumor-Specific Bicistronic Lentiviral Vector, Saeed Ranjbar, Mohammad Sadegh Hashemzadeh, Shahryar Khoshtinat Nikkhoi, Alireza Farasat, Mahdi Tat, Majdedin Ghalavand, Ruhollah Dorostkar
Turkish Journal of Biology
Lentiviral gene delivery is now considered as a major candidate in the future of cancer therapy. To avoid common side-effects associated with methods of cancer therapy, the survivin promoter shows great promise due to its high expression level in multiple cancers. In this research, using a 2A peptide, Noxa was coexpressed with hemagglutinin neuraminidase (HN) driven by the survivin promoter (Surp). Coding genes of Noxa and HN were connected to the upstream and downstream of a 2A peptide using SOE PCR. After cloning of Noxa-2A-HN and Surp into the lentiviral vector, the final construct was transfected to SK-BR3 tumor cells …
Changes In The Levels Of Liver Hsp70, Plasma Nitric Oxide, And The Antioxidative System In An Experimentally Induced Endotoxemia Mouse Model And The Role Of Reduced Glutathione, Temel İlgün, Kezban Yildiz Dalginli, Canan Gülmez, Onur Ataki̇şi̇
Changes In The Levels Of Liver Hsp70, Plasma Nitric Oxide, And The Antioxidative System In An Experimentally Induced Endotoxemia Mouse Model And The Role Of Reduced Glutathione, Temel İlgün, Kezban Yildiz Dalginli, Canan Gülmez, Onur Ataki̇şi̇
Turkish Journal of Biology
Endotoxin molecules in lipopolysaccharides are among most important molecules that initiate a cascade of events in sepsis/endotoxemia. Lipopolysaccharide exposure may result in strong immune responses, disrupt the intracellular oxidant/antioxidant balance, and cause excessive reactive oxygen species generation. The purpose of the study was to examine if reduced glutathione (GSH) has a protective role against lipopolysaccharides. The effects of lipopolysaccharide (LPS) or GSH alone or in combination on the levels of the plasma antioxidant system, NO, and liver HSP70 were investigated. A total of 100 Swiss albino mice were divided into 4 groups as Group I (control), Group II (20 μg/kg …
Cytotoxicity And Antibiofilm Activity Of Sio2/Cellulose Derivative Hybrid Materials Containing Silver Nanoparticles, Tsvetelina Angelova, Nadezhda Rangelova, Veselina Uzunova, Nelly Georgieva, Tonya Andreeva, Albena Momchilova, Rumiana Tzoneva, Rudolf Müller
Cytotoxicity And Antibiofilm Activity Of Sio2/Cellulose Derivative Hybrid Materials Containing Silver Nanoparticles, Tsvetelina Angelova, Nadezhda Rangelova, Veselina Uzunova, Nelly Georgieva, Tonya Andreeva, Albena Momchilova, Rumiana Tzoneva, Rudolf Müller
Turkish Journal of Biology
Hybrid materials based on tetraethyl orthosilicate (TEOS), hydroxypropyl cellulose (HPC), or hydroxypropyl methyl cellulose (HPMC) with silver nanoparticles were synthesized. They were analyzed and characterized using differential thermal analysis and thermogravimetry, atomic force microscopy, and static contact angle measurements. It was experimentally demonstrated that the silver-doped hybrid materials have pronounced antibacterial behavior by studying the reduction of Pseudomonas aeruginosa PAO1 biofilm formation on the tested materials. The results revealed biofilm reduction of 35.7% and 30% by SiO2/HPC/2.5% Ag and SiO2/HPMC/2.5% Ag hybrid materials, respectively, compared to the control. Cytotoxicity of examined materials and actin cytoskeleton organization of fibroblasts seeded on …
Comparison Of Phototoxic Effects Of Hypericin-Mediated Photodynamictherapy In Ht-29 And Caco-2 Colon Cancer Cells, Aysun Kiliç Süloğlu, Güldeni̇z Selmanoğlu, Şükran Yilmaz, Hande Canpinar
Comparison Of Phototoxic Effects Of Hypericin-Mediated Photodynamictherapy In Ht-29 And Caco-2 Colon Cancer Cells, Aysun Kiliç Süloğlu, Güldeni̇z Selmanoğlu, Şükran Yilmaz, Hande Canpinar
Turkish Journal of Biology
Hypericin (HYP) is a plant-derived photosensitizer. HYP is preferentially taken up by tumor cells. We designed this study to compare HYP-mediated photodynamic therapy (PDT) in HT-29 and Caco-2 colon cell lines. Cells were treated with 0.04, 0.08, and 0.15 μM HYP concentrations and irradiated. The effect of HYP on metabolic profiles, alterations in lactate dehydrogenase (LDH) leakage, and cell cycle progression was investigated for the first time. Changes in glucose consumption, lactate production, and LDH leakage were analyzed. HYP-induced cell death was quantified by double staining (acridine orange/propidium iodide) and alterations in cell cycle regulation were analyzed with flow cytometry …
Enhancement Of Direct Shoot Regeneration And Determination Of Bioactivesecondary Metabolites In Leaves Of Galega Officinalis L., Fatma Pehli̇van Karakaş, Günce Ci̇ngöz, Arzu Türker
Enhancement Of Direct Shoot Regeneration And Determination Of Bioactivesecondary Metabolites In Leaves Of Galega Officinalis L., Fatma Pehli̇van Karakaş, Günce Ci̇ngöz, Arzu Türker
Turkish Journal of Biology
Galega officinalis L. is a medicinal plant being used as a galactagogue and an antidiabetic in folk medicine. A new effective micropropagation protocol was established from leaf, stem, node, and root explants of G. officinalis for in vitro plant regeneration and callus induction systems. Murashige and Skoog (MS) medium supplemented with 55 various concentrations and 4 different combinations [benzyl adenine (BA)/naphthalene acetic acid (NAA), BA/indole-3-acetic acid (IAA), thidiazuron (TDZ)/indole-3-butyric acid (IBA), and TDZ/IAA] of plant growth regulators was used for the regeneration systems. The regenerated shoots were observed on only nodal explants through direct organogenesis at the 35th day. The …
The Effects Of Nutrient And Macronutrient Stress On Certain Secondary Metabolite Accumulations And Redox Regulation In Callus Cultures Of Bellis Perennis L., Günce Ci̇ngöz, Fatma Pehli̇van Karakaş
The Effects Of Nutrient And Macronutrient Stress On Certain Secondary Metabolite Accumulations And Redox Regulation In Callus Cultures Of Bellis Perennis L., Günce Ci̇ngöz, Fatma Pehli̇van Karakaş
Turkish Journal of Biology
The main purpose of this study was to investigate the effects of nutrient and macronutrient stress on complex variations in the phenolic profile and antioxidant status of Bellis perennis L. callus cultures. To obtain stress conditions, callus cultures were grown under insufficient nutrient conditions (different strengths of MS medium) or under conditions of limiting calcium (-Ca), magnesium (-Mg), or both (-Ca & -Mg). Phenolic content of callus cultures obtained from different stress treatments was detected quantitatively by LC-ESI-MS/MS analysis. The major compound was chlorogenic acid in all treatments. The accumulation of hesperidin, rutin hydrate, and chlorogenic acid was significantly induced …
Carbohydrate Deprivation Upsurges The Expression Of Genes Responsible For Programmed Cell Death In Inflorescence Tissues Of Oil Palm (Elaeis Guineensis Jacq.), Walter Ajambang, Hugo Volkaert, Sudarsono Sudarsono
Carbohydrate Deprivation Upsurges The Expression Of Genes Responsible For Programmed Cell Death In Inflorescence Tissues Of Oil Palm (Elaeis Guineensis Jacq.), Walter Ajambang, Hugo Volkaert, Sudarsono Sudarsono
Turkish Journal of Biology
The aim of this present study was to analyze global gene expression from a developing inflorescence of oil palm under complete defoliation stress. We used the Illumina platform to sequence six cDNA libraries of defoliated and nondefoliated tissues. The frequency of differentially expressed genes (DEGs) decreased with increasing fold change at P ≤ 0.05. Gene ontology analysis revealed that about 15% of all DEGs were responsible for stress response while 13.9% of genes were responsible for cell organization and developmental processes. DAVID functional analysis clustering of DEGs identified a higher enrichment score (ES) for cellular response to stress (ES = …
Trpc1 Ion Channel Gene Regulates Store-Operated Calcium Entryand Proliferation In Human Aortic Smooth Muscle Cells, Yasemi̇n Erac, Cigdem Selli, Meti̇ner Tosun
Trpc1 Ion Channel Gene Regulates Store-Operated Calcium Entryand Proliferation In Human Aortic Smooth Muscle Cells, Yasemi̇n Erac, Cigdem Selli, Meti̇ner Tosun
Turkish Journal of Biology
This study investigates whether the reciprocal changes in transient receptor potential canonical (TRPC)1 and TRPC6 expressions, which have previously been observed in aging rat aorta, are functional in store-operated calcium (SOC) entry and proliferation in human vascular smooth muscle cells. TRPC1 levels were modulated via silencing and overexpression vectors in human primary aortic smooth muscle cells. Following TRPC1 gene modulation, TRPC1 and TRPC6 expression levels were measured using quantitative real-time RT-PCR. In functional analyses, real-time changes in intracellular calcium levels and cell proliferation were determined. Microarray analysis was performed to identify genes associated with functional alterations following TRPC1 silencing. TRPC1 …
Pepper (Capsicum Annuum L.) Anther Culture: Fundamentalresearch And Practical Applications, Teodora Popova Irikova, Spyridon Kintzios, Stanislava Grozeva, Velichka Rodeva
Pepper (Capsicum Annuum L.) Anther Culture: Fundamentalresearch And Practical Applications, Teodora Popova Irikova, Spyridon Kintzios, Stanislava Grozeva, Velichka Rodeva
Turkish Journal of Biology
To create new varieties resistant to biotic and abiotic stress factors that possess high productivity and improved fruit quality, breeders require modern breeding methods and techniques. Double haploid (DH) lines are an invaluable breeding element due to their guaranteed, completely homozygous nature. One of the fastest methods for obtaining DH lines is anther culture. Microspore embryogenesis provides considerable opportunities in the areas of breeding and biotechnology and represents a model system for fundamental biological research. The effectiveness of pepper anther culture is still low, which affects the application of DH lines in breeding programs. A detailed study of the anther …
A New Reliable And Sensitive Nested Pcr Assay Based On The Human Sry Gene For Detection Of Interspecific Chimeras, Jie Yu, Kaijing Li, Main Huang, Liping Guan, Min Zhang, Weihua Li, Jianfa Huang, Ting Luo, Wencong Wang, Bikun Xian, Xing Liu, Bing Huang
A New Reliable And Sensitive Nested Pcr Assay Based On The Human Sry Gene For Detection Of Interspecific Chimeras, Jie Yu, Kaijing Li, Main Huang, Liping Guan, Min Zhang, Weihua Li, Jianfa Huang, Ting Luo, Wencong Wang, Bikun Xian, Xing Liu, Bing Huang
Turkish Journal of Biology
Although interspecific chimeras can be identified using laborious techniques, more accurate and rapid detectable methods need to be established. The present study develops a nested polymerase chain reaction (nPCR) assay to detect human-mouse chimeras. A set of previously validated outer primers, specific to the human SRY gene, was used for conventional one-step PCR, while the inner primers for nPCR were designed. The specificities of PCR and nPCR assays were examined; both primer sets yielded PCR amplification products from male human epidermis-derived mesenchymal stem cell-like pluripotent cell DNA but no amplification products from negative control DNA. The sensitivity of this nPCR …
Agrobacterium-Mediated Transformation Of Five Inbred Maize Lines With The Brittle 2 Gene, Thuy Ninh Nguyen, Thi Luong Tran, Duc Thanh Nguyen
Agrobacterium-Mediated Transformation Of Five Inbred Maize Lines With The Brittle 2 Gene, Thuy Ninh Nguyen, Thi Luong Tran, Duc Thanh Nguyen
Turkish Journal of Biology
The Brittle 2 (Bt2) gene encodes a small subunit of ADP-glucose pyrophosphorylase (AGPase) enzyme, one of the enzymes that play an important role in endosperm starch biosynthesis. We carried out a study to transfer the Bt2 gene into several inbred maize lines and regenerate transgenic maize plants possessing the transgene, which can be used to further study the role of the Bt2 gene in starch biosynthesis. Agrobacterium tumefaciens strains C58 and EHA105 harboring the transformation vector pCambia2300/Ubi-Bt2-Nos were used to transfer the Bt2 gene into five inbred maize lines, H14, H26, H240, H95, and N18. Transformed calli were selected on …