Digital Commons Network^™

Chronic Stress And Well-Being: Testing Mindfulness As A Proposed Method To Decrease Stress In Parents Of Children With Autism Spectrum Disorder, Madeline M. Gillies

Theses and Dissertations

Parenting is a demanding role that encompasses many responsibilities and challenges. When a child is diagnosed with autism spectrum disorder, there are additional demands on the parent's time, energy, and financial resources, which likely leads to increased levels of chronic stress for parents. This dissertation examines the differences in chronic stress levels between parents who have children with autism and parents who do not, using hair cortisol concentration (HCC) as a benchmark. This dissertation also explores reducing parental stress by introducing a mindfulness intervention. Participants (n = 68) were recruited and divided into two groups: Parents of children who have …

Engineering Cell-Free Protein Synthesis Biosensors For Point Of Care Use, Tyler Jordan Free

Theses and Dissertations

Diagnostic tests can dramatically improve and save lives. Survival rates and patient outcomes related to cancer and other diseases can be improved when routine testing guides personalized treatment. Highly accurate laboratory tests exist for thousands of biomarkers, including those of interest in this dissertation, but cost and complexity limit access to life-saving lab tests in many cases throughout the world. To fill this need and increase accessibility of diagnostics, in this report we report our engineering advancements towards portable biosensors for colorimetric detection of glutamine, endocrine disrupting compounds (EDC), and pathogenic RNA which are key biomarkers in cancer treatment monitoring, …

Using Neural Networks To Reconstruct Temperature From Fluorescent Data For Use In Bio-Microfluidics, Jacob G. Kullberg

Theses and Dissertations

It has been theorized that Quantum Dots (QD) can solve the temperature sensor issue with microfluidic devices. Quantum Dots are nano-sized semiconducting crystals with a radius of an excited electron-hole pair. When a QD is excited by a short wavelength light, the QD produces fluorescent light. As the temperature of the dot changes, the dot expands and shifts the fluorescent spectrum. Quantum Dots are small enough to be placed inside the microfluidic devices, and a spectrometer can measure the change in fluorescent data. This fluorescent data can be used to create fluorescent images corresponding to the QD temperature. These fluorescent …

Laying Framework For A Novel In Vitro Eukaryotic System To Measure Rate Of Transcription Elongation, John Lucas Ramirez

Theses and Dissertations

Within each cell, genetic material is stored in the form of DNA. This DNA is compacted around proteins to form higher order structures called chromatin. The basic unit of chromatin is a histone protein octamer bound with DNA called a nucleosome. A nucleosome consists of eight core proteins, four pairs of histone proteins H2A, H2B, H3, and H4, wrapped by DNA. The DNA wraps around the histone protein roughly 1.7 times, helping facilitate the compaction of DNA within the nucleus of the cell. The location of nucleosomes makes genetic material that encodes various elements, such as promoters or enhancers, accessible …

Cellular Sorting Of The Dictyostelium Discoideum Slug, Erin Flowerday

Theses and Dissertations

Dictyostelium discoideum (Dd) cells are amoeba cells that feed on soil or plant leaf matter bacteria. They divide freely in the presence of food. When starved, cell division is ended, and cells transition from unicellular to multicellular organisms initiated by a chemoattractant, cyclic adenosine monophosphate (cAMP). This study intricately examines cellular sorting within the Dd slug, focusing on individual cell dynamics. Represented as oriented ellipsoids, these cells maintain constant volume through a viscoelastic structure. Applying force to specific semi-axes reveals predictable deformations, highlighting the relationship between force and deformation rate. Manipulating damping coefficients and spring constants uncovers insights into cell …

Connecting Hiv-1 Viral Protein R Polymorphisms To Changes In Inflammatory Signals And Efficacy Testing Of Essential Oils As Disinfectants Against Sars-Cov-2, J. Brandon Lopez

Theses and Dissertations

Human Immunodeficiency Virus (HIV-1) is the causative agent for acquired immunodeficiency syndrome (AIDS). Different strains of HIV-1 have been linked to a long term non-progressor (LTNP) or rapid progressor (RP) form of disease depending on rate of AIDS progression. Viral protein R (Vpr) is a viral protein that has been linked to varying rates of AIDS progression depending on the sequence. In this work, the effects of two polymorphisms, a LTNP and a RP, on different cells are studied. The LTNP virus was linked to lower release of pro-inflammatory cytokines, such as TNF and IL-6, and an increase in apoptotic …

Design And Validation Of Sensing Knee Sleeve Rehabilitation Device Utilizing Large Deflection Strain Gauges, Kurt Christian Jensen

Theses and Dissertations

Total knee arthroplasty (TKA) procedures are increasingly common in the US and globally. However, rehabilitation outcomes may be negatively affected by limited access to physical therapy and patients' tendency to not fully adhere to recommended exercises between therapy sessions. This study aims to develop a remote system to aid rehabilitation, building upon recent sensor development at Brigham Young University, providing physical therapy-like biofeedback during unsupervised at-home exercises. The system developed by this research consists of three primary components: (1) a sensor array for measuring leg/knee biomechanics, (2) a machine learning algorithm to predict knee flexion angle from the sensor data, …

Acousto-Dielectric Tweezers Enable Independent Manipulation Of Multiple Particles, Liang Shen, Zhenhua Tian, Kaichun Yang, Joseph Rich, Jinxin Zhang, Jianping Xia, Wesley Collyer, Brandon Lu, Nanjing Hao, Zhichao Pei, Chuyi Chen, Tony Jun Huang

Faculty Publications

Acoustic tweezers have gained substantial interest in biology, engineering, and materials science for their label-free, precise, contactless, and programmable manipulation of small objects. However, acoustic tweezers cannot independently manipulate multiple microparticles simultaneously. This study introduces acousto-dielectric tweezers capable of independently manipulating multiple microparticles and precise control over intercellular distances and cyclical cell pairing and separation for detailed cell-cell interaction analysis. Our acousto-dielectric tweezers leverage the competition between acoustic radiation forces, generated by standing surface acoustic waves (SAWs), and dielectrophoretic (DEP) forces, induced by gradient electric fields. Modulating these fields allows for the precise positioning of individual microparticles at points where …

Nanoparticles To Deliver Stuff, William Pitt

BYU Research Development Office Research Networking Conference

Areas of Interest: Biomedical Engineering, Drug Delivery, Cancer and Infections, Polymer Science, Colloid and Surface Science

Structural Characterization Via Ion Mobility-Mass Spectrometry, Kenny Lee

BYU Research Development Office Research Networking Conference

Areas of Interest: Structural biology, Isomer/conformer characterization, Gas-phase ion chemistry

Swallowable Capsules For Precision Probiotics And Future Gut Health Monitoring, Benjamin Terry, John M. Chaston, Scott Weber

BYU Research Development Office Research Networking Conference

Areas of Interest: Gastrointestinal disease and therapy, medical devices, surgical tools

Muscle Transplants For Treating Volumetric Muscle Loss Injury, Jake Sorensen

BYU Research Development Office Research Networking Conference

Areas of Interest: Skeletal Muscle Physiology/Pathology, Stem Cell Biology, Traumatic Musculoskeletal Injuries

Personalized Medicine In Pediatric Oncology, Jared Barrott

BYU Research Development Office Research Networking Conference

Areas of Interest: Personalized medicine to treat cancer, Genomic sequencing methods, Pharmacology and drug testing, Antibody drug conjugates, 3D tumor modeling, ChIP sequencing

Liquid Biopsies For Cancer Detection And Monitoring, Amber Gonda

BYU Research Development Office Research Networking Conference

Areas of Interest: Cancer Biology, Tumor Microenvironment, Tumor Immunology, Solid Tumor Detection and Monitoring, Liquid Biopsy

Finding Therapeutic Targets From Big (Biological) Data, Brett E. Pickett

BYU Research Development Office Research Networking Conference

Areas of Interest: Cancer, Infections, RNAseq, Disease, Treatments, Diagnostics

Kairns Solutions: Stacking Molecular Insights For Epilepsy Control, Matthew H. Bailey

BYU Research Development Office Research Networking Conference

Areas of Interest: Genomics epidemiology, Cancer Biology, big data analysis, writing grants, discovery, and statistics

Electron Microscopy Facility, Felipe Rivera

BYU Research Development Office Research Networking Conference

Scanning Electron Microscopy, Transmission Electron Microscopy, Focused Ion Beam micro-machining, XEDS, CL, EBSD, EELS, etc.

Drug Discovery And Pig Kidney Transplants, Dario Mizrachi

BYU Research Development Office Research Networking Conference

Areas of Interest: Protein Engineering, Biotechnology Applications

Uncertainty Quantification & Radical Interdisciplinarity, Douglas Cook

BYU Research Development Office Research Networking Conference

Areas of interest: Limitations of scientific models; sensitivity analyses; theory-based and statistical modeling; systems thinking, systems analysis

Improving Battery Electrolytes With Operando Optical Diagnostics, Jason M. Porter

BYU Research Development Office Research Networking Conference

Areas of Interest: Optical diagnostics, energy conversion and storage, heat transfer

Optimization Of Mass Spectrometry-Based Methods For Low-Input And Spatial Proteomics, Andikan Jones Nwosu

Theses and Dissertations

Eukaryotic cells are highly heterogeneous. These cells are arranged into different compartments, carrying out separate functions and facilitating biological processes. Proteins are the effector biomolecules targeted to subcellular locations that help fulfill specific tasks in living organisms. Spatial proteomics can help unravel molecularly how protein abundance and localization are altered in cells, which is not feasible in traditional bulk-scale proteomics. To achieve this, our lab has developed a miniaturized sample processing platform called nanoPOTS, reduced separation columns' inner diameter to increase ionization efficiency and concentrate analytes for mass spectrometers and optimized data acquisition modes for increasing proteome coverage in spatial …

Peer Mentoring Program For Refugee And Newcomer Children To Increase Resilience, Chloe Skyla Cooksey

Theses and Dissertations

The refugee and newcomer population faces many challenges as they arrive in their host country. Many individuals experience varying degrees of trauma in their country of origin. Trauma can lead to poor mental health outcomes and poor adjustment in host countries. Refugee children in particular may experience post-traumatic stress disorder, depression, and anxiety as a result of traumatic experiences. Children can be resilient when given opportunities to create relationships and gain confidence. These opportunities can be fostered through various avenues, one of which is the school environment. Refugee students can benefit from various supports provided to them in the school …

Impacts Of Managed Honeybee Apiaries On Native Bee Populations, Trapping Efficiencies Of Bee Sampling Methods, And Drone Based Floral Cover Analysis In High Elevation Tall Forb Communities In Central Utah, Nicholas Val Anderson

Theses and Dissertations

This dissertation investigates the impact of honeybee introductions on native bee populations, evaluates the efficiency of traditional bee sampling and monitoring methods, and explores the use of drone technology for assessing floral resources on federal lands in the United States. Federal lands, managed for multiple uses, present a valuable opportunity for the summer pasturing of honeybees. These lands produce vast floral resources and are largely free from pesticides and other pollutants that could weaken honeybee populations. The potential use of this resource is significant given the economic and agricultural concerns presented by declining pollinator trends and Colony Collapse Disorder (CCD). …

Functional & Evolutionary Analysis Of Tbx2a And Tbx2b In Zebrafish Heart Development, Maliha Tasnim

Theses and Dissertations

Congenital Heart Disease (CHD) is the most common birth defect, affecting approximately 1% of live births. This dissertation explores the genetic mechanisms underlying CHD, focusing on the T-box transcription factors Tbx2a and Tbx2b in zebrafish. Utilizing advanced bioinformatics techniques, we reconstructed the ancestral sequences of these genes to understand their evolutionary trajectory and functional divergence. Our analysis highlights the evolutionary conservation of the DNA-binding domain in tbx2a and tbx2b, suggesting its crucial role in maintaining correct gene expression patterns during development. We observed unique and shared amino acid substitutions in these proteins, indicating potential adaptive changes and conserved functions. Comparative …

3d Printed Mounts For Liquid Microdroplet Resonators, Parker A. Awerkamp

Theses and Dissertations

Liquid microdroplet resonators provide an excellent tool for optical studies due to their innate smoothness and high quality factors, but they can be difficult to control. By using 3D printed mounts to support the droplets, we can obtain precise control over the droplet geometries and positions. We here present our work with oil, water, and ice microdroplets, as well as tools required to enable their study. We first present methods for creating 3D printed mounts for oil microdroplet resonators. The mounts enable precise positioning of the droplets relative to a tapered optical fiber. The oil microdroplet resonators exhibited quality factors …

Eugenics And Feminism: The Reflections Of Two Ideologies In The Reproductive Policies Of The Nazi Regime, The Reunification Period, And Present-Day Germany, Sydney Reil

Undergraduate Honors Theses

German abortion law is restrictive for a Western European nation of strong economic standing and with relatively liberal social policy. Additionally, Germany is one of few Western European nations without an embryopathy indication, which allows for exceptional abortions in cases of fetal defects. The law (as it stands in 2024), has been immeasurably shaped by two ideologies, eugenics and feminism, and by modern Germany’s consideration of both the ideologies themselves, and of how they influenced the reproductive policies of the twentieth century. The purpose of this study is to demonstrate the influences of eugenics and feminism on German reproductive law …

An Applied Mental Health Course And Student Well-Being, Alison Nicole Anglen

Theses and Dissertations

Addressing mental health concerns and promoting well-being is imperative for university students to function optimally and to succeed academically. The demand for mental health care is exceeding the capacity of traditional counseling center resources at universities, suggesting the need for innovative interventions that can serve a broader scope of students. There is emerging evidence suggesting that mental health can be addressed, and well-being improved through psychoeducation and applied skills taught in classroom settings. Objectives: To examine a Mental Health and Well-Being course at a large, private religious university and its relationship to students’ well-being. This will be measured by assessing …

The Impact Of The Covid-19 Pandemic On The Incidence Of Sexual Assaults And Victim Services, Jessica Hunter

Student Works

The Impact of the Covid-19 Pandemic on the Incidence of Sexual Assaults and Victim Services

Purpose: The purpose of this literature review is to discuss incidents/rates of sexual assaults (SA), access to or accessibility of SA victim services, and the utilization of SA services during the COVID-19 pandemic.

Background: In the U.S., 1 in 5 women experience rape or attempted rape, while 1 in 4 men experience some form of sexual violence in their lifetime. Additionally, SAs affect roughly one-third of the world's population, making SA a prominent problem worldwide.

Methods: Electronic searches in CINHAL and MEDLINE were performed using …

High School Sport Participation: Effects On Students' Grit And Resilience, Rory Kekoa-Israel Waldman

Theses and Dissertations

Adolescence is a time of transition, growth, and identity exploration. Often this great time for growth is accompanied with an increase in stressors that can be detrimental to success and health, without the necessary coping mechanisms. It is a period of life where resilience and grit are extremely important. A post-traumatic growth definition for resilience allows for the possibility of a steeling effect, in which the resilience of an adolescent can grow and develop. Sport provides an ideal environment for the development of both grit and resilience by providing a safe environment for adversity to occur without detrimental consequences. In …

Takiri Ake Nga Haeata: Lighting A Path To Increased Indigenous Leadership In Aotearoa, Kaylee Ihimaera

Theses and Dissertations

This thesis addresses the underrepresentation of indigenous Māori people in leadership positions and pursues the need to develop a Māori leadership framework aimed at empowering the next generation of indigenous people. Several frameworks have attempted to exhibit Māori leadership dynamics, each offering unique insights into the cultural and relational aspects of Māori leadership. However, existing frameworks often exhibit limitations in fully encapsulating the breadth of Māori leadership values and practices, necessitating the development of a comprehensive Māori leadership model independent of Western frameworks. Through an extensive review of existing literature and engaging with Māori experts, I synthesized key insights to …