Digital Commons Network^™

Cytological Investigations Of Eremosphaera Viridis De Bary, Richard Allen Valentino

Masters Theses

Eremosphaera viridis de Bary was studied with light microscope techniques to determine the method of cytokinesis and to explain the ontogeny of the membranous ring separating the mature daughter cells in the intact sporangium.

It is proposed that cytokinesis in this organism is phylogenetically advanced and that the separation of mature daughter cells involves the sequential deposition of two cell walls. The first wall develops centripetally by the addition of membrane subunits. The resulting cleavage furrow is oriented by transverse microtubules found along the cell division plane. The newly formed walls separate the two daughter cells and give rise to …

Saxicolous Bryophytes Of Coles And Clark Counties, Terri M. Simon

Masters Theses

No abstract provided.

Studies On The Genus Streptococcus, Janet Lee Storey

University of the Pacific Theses and Dissertations

The streptococci, as a group, are gram positive cocci which occur in pairs or chains; they are nonmotile, nonsporing, and catalase negative. Most species are facultative anaerobes, and a few are obligate anaerobes. They are chemorganotrophs producing lactic acid as an end product of glucose metabolism. The G + C (guanine + cytosine) content reported for 15 species is 33-42% (Diebel and Seeley, 1974). The genus Streptococcus includes a large number of saprophytic, pathogenic and non pathogenic species. Many of these bacteria are members of the normal body flora. However, man is very susceptible to the pathogenic members, and no …

Louisiana Agriculture Magazine, Winter 1977-1978, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

Modeling Soil Water Movement Into Plant Roots, D. C. Slack, C. T. Haan, Larry G. Wells

Biosystems and Agricultural Engineering Faculty Publications

Mathematical model was developed which describes uptake of water by plant roots as a function of leaf and soil water potentials. The model was used to estimate transpiration from corn grown in a controlled environment under soil drying conditions. The model predicted daily transpiration quite well for the period modeled.

A Morphological And Histological Study Of The Hepatitis B Antigen Treated Nicotiana Sylvestris And Nicotiana Tabaccum Variety Xanthi, Jeanne Elisabeth Moldenhauer

Master's Theses

No abstract provided.

The Fluorescence Microscopic Study Of The Infection Of Saccharomyces Cerevisae (Yeast) Protoplasts With Tobacco Mosaic Virus, Madhubhai. Patel

Master's Theses

No abstract provided.

A Biosystematic Investigation Of Medically Important Yeasts, Nancy Christiansen Ballot

University of the Pacific Theses and Dissertations

The purpose of this study is twofold: (1) conduct an epidemiological survey of yeasts found in clinical material; and (2) suggest an identification scheme that would identify yeasts of medical importance in the shortest possible time.

Molecular Mobilities Of Soluble Components In The Aqueous Phase Of Chromaffin Granules, Edward P. Richards, Robert R. Sharp

Journal Articles

NMR relaxation times have been used to characterize molecular motion and intermolecular complexes in the aqueous phase of bovine chromaffin granules. Partially relaxed '3C and proton spectra have been obtained at 3 and 25°C. T1 measurements of five protonated carbons on epinephrine (C:, Cs, C6, CHOH and NCH3) give a correlation time of 0.15 (10 -9) s at 25°C for the catechol ring and methine carbon, while the effective correlation time for the NCH3 group is somewhat shorter due to its internal degree of rotational freedom. Resonances of protonated carbons on the soluble protein chromogranin give very similar correlation times: …

Analysis Of The Carbon-13 And Proton Nmr Spectra Of Bovine Chromaffin Granules, Edward P. Richards, Robert R. Sharp

Journal Articles

Natural abundance carbon-13 and proton NMR spectra of bovine chromaffin granules have been obtained and analyzed using computer simulation techniques. High resolution spectra show the presence of a fluid aqueous phase containing epinephrine, ATP and a random coil protein. The protein spectrum conrains unusually intense resonances due to glutamic acid and proline and has been simulated satisfactorily using the known amino acid composition of chromogranin A.

The lipid phase of chromaffin granules gives rise to intense, but very broad, resonances in the carbon-13 spectrum. Protons in the lipid phase are also observable as a very rapid component of the proton-free …

Inseticidas Sistémicos E Endrin Aplicados No Controle De Elasmopalpus Lignosellus, E Seus Efeitos Sobre A Soja, Elvis A. Heinrichs

Department of Entomology: Faculty Publications

Foi conduzido um experimento a campo, na Estação Experimental Agronômica da UFRGS, em Guaíba, RS, com o objetivo de determinar o efeito de vários inseticidas no controle da “broca-do-colo,” Elasmopalpus lignosellus (Zeller, 1848). Os tratamentos aplicados foram: 2 dosagens de Carbofuran no tratamento de sementes; 2 dosagens de Carbofuran, Aldicarb e Disulfoton granulado, aplicado no sulco na época do plantio; e 2 tratamentos de Endrin de 1 e 3 pulverizações no período de pós-emergência. Os resultados indicaram que apenas dois tratamentos com Endrin controlaram a broca. Rendimentos altos nos tratamentos com Endrin e Carbofuran granulado foram atribuídos ao controle da …

Hormdne-Inducible Casein Messenger Rna In A Serum-Free Organ Culture Of Whole Mammary Gland, P. M. Terry, M. R. Banerjee, R. M. Lui

School of Biological Sciences: Faculty Publications

The whole second thoracic mammary gland of estradiol-17β + progesterone primed 3- to 4-week-old BALB/c female mice was induced to pregnancy-like lobulo-alveolar morphogenesis after 6-day cultivation in a serum-free culture medium containing a "growth promoting" hormone mixture, insulin + prolactin + growth hormone (somatotropin) + estradiol + progesterone. No radioimmunologically detectable casein was present in these glands. Subsequent cultivation for another 6 days in a "lactogenic" medium with the hormones insulin + prolactin + cortisol produced abundant milk-like secretory material in the alveolar lumen. RNA of the mammary gland after estradiol + progesterone priming or cultivation in the "growth-promoting" medium …

Absence Of Spermine In Filamentous Fungi, Kenneth W. Nickerson, Larry D. Dunkle, James L. Van Etten

Papers in Microbiology

Polyamines were examined in several yeasts and filamentous fungi. Whereas putrescine, spermidine, and spennine were present in the yeasts, spernine was not detected in any of the filamentous fungi.

The Orientation Of Asplanchna Brightwelli In A Weak Horizontal Magnetic Field, Priscilla Kathleen Dale

Master's Theses

No abstract provided.

The Partial Purification And Characterization Of A Soluble Activator Of The Cation Transport Adenosinetriphosophatase In Bovine Caudate Nucleus, Paul Culver

University of the Pacific Theses and Dissertations

To many investigators, these observations suggested the existence of a molecular “pump” mechanism responsible for the maintenance of the ionic gradients. Extensive research over the last twenty years has resulted in the assignment of this role to the magnesium-dependent, sodium- and potassium-stimulated adenosine triphosphatase, first reported by Skou in 1957. This membrane-bound enzyme is the subject of the research presented in this thesis.

The Biosynthesis Of Mannitol And Mannitol 1-Phosphate In Staphylococci, Kenneth Edwards

Master's Theses

No abstract provided.

Direct Chemical Evidence For A Supramolecular Protein Structure In The Nuclear Envelope, David Lee Cochran

Theses and Dissertations

Protein subunit structure in the nuclear envelope has not been previously described. Covalent crosslinking of polypeptides provides a method for studying the subunit structure of protein-rich systems. In this study, several polypeptide crosslinking methods have been used to determine associations among polypeptides in isolated chicken erythrocyte nuclear envelope. Interchain associations that exist in the isolated membrane were fixed by crosslinking the polypeptides in the isolated nuclear envelope. Crosslinked and uncrosslinked polypeptides were resolved by electrophoresis in a dissociating detergent system. Each agent caused distinctive alterations in the gel pattern. Certain bands diminished and disappeared while new bands of two or …

Characterization Of The Myosin-Phosphorylating System In Normal Murine Astrocytes And Derivative Sv40 Wild-Type And A-Mutant Transformants, S. P. Scordilis, J. L. Anderson, R. Pollack, R. S. Adelstein

Biological Sciences: Faculty Publications

Myosin and myosin light-chain kinase have been isolated and characterized from small quantities of normal and SV40-transformed, murine astrocytic neuroglial cells in culture and from intact normal mouse brain. Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of the astrocyte myosins revealed a heavy chain of 200,000 daltons and two light chains of 20,000 and 15,000 daltons. These myosins are similar to other cytoplasmic myosins. The astrocyte 20,000-dalton light chain can be phosphorylated by an endogenous myosin light- chain kinase which has properties similar to those of the myosin light-chain kinase found in human platelets. No differences were detected in either the …

Duck Plague Virus An Investigation Of Cytopathic Effects, In-Vitro Host Range, And Inhibition By Phosphonoacetic Acid, Ronald Attanasio

Biological Sciences Theses & Dissertations

Duck plague is a hemorrhagic, necrotic disease found exclusively in the order Anseriformes, family Anatidae (ducks, geese, swans). The etiologic agent has been reported to be a herpes-like virus. The Holland strain of duck plague was found to induce cytopathic effects in cell culture (perinuclear vacuolation followed by lysis). A simple reproducible plaque assay for quantitating the virus was developed. Under conditions of the plaque assay, one-hit kinetics were observed. The host range of duck plague virus in cell culture was found to include the order Galliformes, family Phasianidae (pheasants, quails and partridges). Duck plague virus-induced cell culture cytopathic effects …

Susceptibility At High Inoculum Of Penicillin-Resistant Staphylococcus Aureus To Six Cephalosporins, Andres Suarez, Ramon Del Busto, Edward L. Quinn, Donald Pohlod

Henry Ford Hospital Medical Journal

Six cephalosporins (currently available for clinical use or undergoing clinical trials) were tested by tube dilution method against high inoculum (10⁷ colony forming units) of 37 strains of penicillin resistant Staphylococcus aureus. 3.1 μg/ml of cephalothin inhibited 100% of strains while higher concentrations of cefoxitin, cephapirin, cefamandole, cephalexin and cephradine were required. The percent of strains with minimal inhibitory concentration higher than 3.1 μg/ml ranged between 10.8% (cefoxitin) and 100% (cephradine). These findings support the observation that cephalothin is less susceptible to inactivation than the other cephalosporins. Although the exact clinical implication of these findings has not been established, …

Some Platyhelminth Parasites Of Sirens (Amphibia: Sirenidae) From North America, Daniel R. Brooks, Richard L. Buckner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A new genus and species of digenean and a new species of cestode are described and a previously described species of digenean reported for the first time from Siren intermedia from Illinois. A new genus is proposed for a previously described digenean, and another known species of digenean reported from Siren lacertina in Florida. Progorgodera foliata from S. intermedia has a foliate hind- body characteristic of members of Phyllodistomum and multiple testes arranged in 2 longitudinal rows characteristic of members of Gorgodera. Ophiotaenia sireni from the same host resembles O. filaroides in number of testes, O. amphiumae and O. …

A Review Of The Limnology Of And Water Quality Standards For Lake Mead, Charles R. Goldman

Publications (WR)

1. The waters of Las Vegas Bay, a heavily utilized recreational resource, receive discharges from a variety of municipal and industrial waste sources. The U.S. Environmental Protection Agency has determined, on the basis of numerous studies, that the present water quality violates state and federal standards and constitutes a public nuisance. Consultants have advised the Sewage and Wastewater Advisory Committee that rapid abatement of the alleged pollution conditions can be achieved by an advanced wastewater treatment (AWT) plant.

2. The major problems in Las Vegas Bay are an objectionable water color, excessive turbidity, noxious odors, and oxygen depletion in certain …

Animal Rights: Nih Cat Sex Study Brings Grief To New York Museum, Nicholas Wade

Popular Press Items

No abstract provided.

Louisiana Agriculture Magazine, Fall 1976, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

Observations On Thamnidiaceae (Mucorales). Ii. Chaetocladium, Cokeromyces, Mycotypha, And Phascolomyces., Gerald L. Benny, R. K. Benjamin

Aliso: A Journal of Systematic and Floristic Botany

Four previously established genera of Thamnidiaceae and their recognized species are described and illustrated. Seven species are treated as follows: Chaetocladium jonesii, C. brefeldii, Cokeromyces recurvatus, Mycotypha africana, M. microspora, M. poitrasii (a new combination based on Cokeromyces poitrasii), and Phascolomyces articuloms. Phascolomyces articuloms, the type species of Phascolomyces, was described in 1959 without the preservation of a nomenclatural type as required by the International Code of Botanical Nomenclature, and original material is unknown. A neotype specimen is designated (preserved in RSA) for validation of the genus and species.

Prolonging Oxygen Consumption During Preservation Of Canine Kidneys By The Addition Of Continuous Dialysis, Daniel Dall'olmo, Stanley G. Dienst

Henry Ford Hospital Medical Journal

Kidneys were preserved by continuous pulsatile perfusion and their metabolic rate monitored by O2 consumption. Experiments were carried out to determine if dialyzing the perfusate to supply metabolic substrates would aid in kidney preservation. A control group was compared against two experimental groups at 25°C. Average preservation time before fall off of oxygen consumption differed for each group: control - 8 hours, dialysis with amino acids - 6 hours, dialysis with amino acids and cofactors - 11 hours. Results indicated that, if suitable concentrations of metabolic substrates and cofactors were used, the preserved kidneys maintained higher levels of O2 consumption …

Oral Contraceptives And Myocardial Infarction: Report Of Three Cases And Review Of The Literature, Mohsin Alam, Remigio Garcia, Ellet H. Drake

Henry Ford Hospital Medical Journal

Three cases are presented of young women who had myocardial infarctions possibly due to coronary thrombosis related to oral contraceptive therapy. These patients had none or very few risk factors for coronary artery disease. In two of the patients, selective coronary arteriograms showed normal results on the third and tenth week after the diagnosis was established. The pathogenesis of coronary artery thrombosis in patients receiving oral contraceptives containing estrogen is still controversial, but it appears to be the result of its multifactorial effect on the platelets, coagulation factors, vessel wall, blood pressure, postprandial blood sugar and abnormalities of lipid metabolism.

Cell-Mediated Cytotoxicity Of Jensen Tumor In Sprague-Dawley Rats, Theodore D. Masek

Loma Linda University Electronic Theses, Dissertations & Projects

An in vitro system for the study of the cell-mediated immune response to Jensen Sarcoma (JS) is described. The tumor grew unrestricted and lead to the demise of 80% of successfully challenged Sprague-Dawley rats (persistors) while 20% of challenged rats (regressors) destroyed the tumor by an unknown mechanism(s). Lymphocytes from both persistors and regressors were shown to be cytotoxic for tumor cells in vitro. Pretreatment of tumor target cells with "immune" serum from persistors or regressors had no effect on the in vitro cytotoxicity of lymphocytes from tumor-challenged rats. Pretreatment of lymphocytes with irmnune serum, however, was shown to reduce …

Effect Of Matallic Ions, B-Vitamins, & Amino Acids On The Production Of Trans-2, 3-Epoxysuccinic Acid By Paecilomyces Varioti, Eleanor Ling

Masters Theses & Specialist Projects

The nutritional conditions which permit maximum yield of trans-2,3- epoxysuccinic acid by Paecilomyces varioti NPRL 1123 were investigated. Copper and iron ions were added to decationized fermentation media to determine the optimum concentration and ratio of the ions for the accumulation of trans-2,3-epoxysuccinic arid. P-vitamin and amino acid studies were done by single omission of the growth factors in culture media. Organic acids in the cultures were recovered by ether extraction and identified by gas chromatography.

In addition to t-ans-2,3-epoxysuccinic acid, succinic acid, malic acid, and tartaric acid were also found in culture filtrates. The results indicated that with a …

Biota And Environment Of The Muskegon, Michigan, Combined Industrial And Municipal Wastewater Storage Lagoons, W. Randolph Frykberg

Dissertations

No abstract provided.