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Isolation, Purification And Partial Characterization Of Some Isoenzymes Of Human Serum Alkaline Phosphatase, M. Sue Jones

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by M. Sue Jones on July 18, 1977.

Effect Of Chitosan As A Stabilizer In Hot Sauce, Richard Arnold Schlottmann

LSU Historical Dissertations and Theses

By the use of mild demineralization in the purification of chitin from shrimp shells and optimizing the deacetylation of this chitin, chitosan capable of producing viscosities of 9600 cps were produced. This is substantially higher than the viscosity of the commercial product, whose viscosity is only 1000 cps. The effectiveness of this high quality chitosan as a stabilizer in hot sauces was then investigated. From prelim­inary studies using the commercially produced chitosan, it was concluded that the method of incorporating the gum into the hot sauces was very important. Therefore, three methods of adding the chitosan to the hot sauces …

Louisiana Agriculture Magazine, Summer 1977, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

Meeting Of The Elephant Interest Group At Michigan State University, June 21, 1977, Jeheskel Shoshani

Elephant

Digest of the minutes of the first informal meeting of the Elephant Interest Group at the 57th Annual Meeting of the American Society of Mamalogists (ASM).

The Effect Of Cv-1 Cell Geometry On The Ultraviolet Radiation Sensitivity Of Mammalian Cells In Vitro, Daniel J. Knauer

Masters Theses & Specialist Projects

The effect of cell geometry on the radiosensitivity to ultraviolet (UV) light has been studied in the CV-1 host cell-capacity system. In this system the macromolecular damage incurred by monolayers of cells cultures as the result of exposure to UV radiation is monitored by testing the ability of irradiated cells to support the replication of Herpes simplex virus. The target molecule has been shown to be DNA and is centrally located

Mammalian cells have an absolute requirement for Mg⁺⁺ and Ca⁺⁺ in order to remain attached to artificial substrata. To test the effect of cell geometry on UV …

Limnological Aspects Of Lake Mead, Nevada-Arizona, John R. Baker, James E. Deacon, Thomas A. Burke, Samuel S. Egdorf, Larry J. Paulson, Richard W. Tew, Bureau Of Reclamation

Publications (WR)

Lake Mead is a deep, subtropical, moderately productive, desert impoundment with a negative heterograde oxygen profile occurring during; the summer stratification. investigations of the Boulder Basin of Lake Mead by the University of Nevada were initiated in November 1971. The primary objective of the study was to determine what effects industrial and sewage effluent from the Las Vegas metropolitan area, discharged into Las Vegas Bay, have had on the water quality and limnological conditions of Boulder Basin. Data from the 1975-76 period are presented in detail, with earlier data included in the summaries and discussions. Measurements of water temperature, dissolved …

A Self-Instructional, Multi-Media Approach To Facilitate The Teaching Of The Index Of Nutritional Quality, Claudia Kay Probart

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A review of the literature dealing with nutrition education established the following: (a) that there is a need for nutrition education at the elementary school level; (b) that elementary teachers do not always have the background or motivation to meet this need for nutrition education; and (c) that new educational methods that are easier to understand and more highly motivating are needed.

The Index of Nutritional Quantity (INQ), a nutrient density concept, was developed at Utah State University to answer the need for easily understood and motivating nutrition information. The INQ method of nutrition education has been used and considered …

The Relationship Of Molybdenum To Iron Status In Pregnancy And Anemia In Rats And Humans, Jo Ann Mortensen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Weanling male rats were made anemic and fed diets supplemented with 20 ppm iron and/or 2 ppm molybdenum. A decrease in serum iron was observed in the rats supplemented only with iron and a significant decrease in hemoglobin was observed in rats given no supplementation.

In a second experiment, pregnant female rats were also fed diets supplemented with 20 ppm iron and/or 2 ppm molybdenum. An inverse relationship was apparent between iron and copper in both the serum and the liver of the female rats. The livers of their pups displayed an inverse relationship between molybdenum and copper. Hemoglobin in …

The Development And Evaluation Of A Nutrient Density Based Nutrition Education Program For Elementary Schools, Guendoline Brown

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A behavioral objective based nutrition education program for kindergarten through sixth grade which allowed nutrition education to be integrated into existing classroom programs was developed and evaluated. A nutrient density approach served as the conceptual framework for the program. Nutrient density evaluates the nutrient content of a food in relationship to the caloric content. Materials for student use, teacher use and teacher training were developed.

The program was evaluated in 27 classrooms in nine public elementary schools in Utah and Idaho with 806 student participants. Pre- and post tests as well as classroom evaluations were conducted.

Students, teachers and parents …

Influence Of Corynebacterium Insidiosum On The Water Relations Of Alfalfa, Rastri Dey

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The effect of Corynebacterium insidiosum (McCull) H. L. Jensen on the water relations of its host, alfalfa, was studied by field measurements made on a 6-year-old stand of Ranger alfalfa. Periodic measurements of stomatal conductance, xylem pressure potential Ψ, and root, stem, and leaf conductance were made in relation to soil moisture conditions. Water regain and electrolyte leakage studies were also made to test membrane integrity in diseased plant leaves.

At all times the xylem pressure potential Ψ was found to be much more negative in diseased plants than in healthy plants. Stomatal conductance was found to respond to the …

A Preliminary Investigation Of The Lipid Of Halophilic Bacteria As A Food Additive, Neil F. Collins

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was conducted to evaluate the feasibility of obtaining workable quantities of the unusual ether-linked phosphatidyl glycerophosphate of halophilic bacteria through batch culture of Halobacterium halobium. Once isolated and purified, physical, emulsification, and toxicological properties of the lipid were evaluated with respect to its use as a nonnutritive lipid substitute or an emulsifier by the food industry. These evaluations included physical descriptions, melting properties, hydrophile-lipophile balance (HLB) determination, and oral and intraperitoneal administration of the lipid to laboratory mice.

Seed And Cone Insects Associated With Pinus Monophylla Torr. And Frem., Michael James Jenkins

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Dissection and rearing studies of second-year cones disclosed six species of insects associated with the seeds and cones of singleleaf pinyon pine. Those most commonly encountered were: Dioryctria sp. probably albovittella Hulst, Conophthorus monophyllae Hopkins and Pineus coloradensis Gillette. The three remaining species were of lesser importance. These included the gall midge, Asynapta sp., a minor cone pest and two parasites, one of C. monophyllae, Acerocephala atroviolacea Crawford and an unidentified parasite of Dioryctria, of the family Ichneumonidae.

D. sp. probably albovittella was regarded as the major insect destroying cones and seeds of P. monophylla in …

In Vitro Morphogenesis And Mutation Of Glycine Max L., Thomas Daniel Wilson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Soybean (Glycine max L. c.v. Consoy) tissue cultures were grown in synthetic media containing various concentrations and combinations of minerals, auxins, cytokinins, pH levels, and inorganic growth factors in a systematic procedure to establish optimum media for initiation and maintenance of callus. Further modification of the hormone level was employed to initiate callus morphogenesis. Normally toxic levels of atrazine were established in soybean calli media, and ethyl methanesulfonate (EMS) and X ray-induced mutant calli were selected for atrazine tolerance.

Optimal media for initiation and maintenance of soybean calli were developed and a defined medium was determined that consistently yields …

Multiple Forms Of Deoxyribonucleic Acid-Dependent Ribonucleic Acid Polymerase In Barley Leaves (Hordeum Vulgare L.), Sachchidananda Javali

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Ribonucleic acid polymerases (E.C. 2.7.7.6) have been solubilized form whole green leaves of barley (Hordeum vulgare L.). Three major forms of the solubilized enzyme have been isolated by sonication in high ionic strength buffers, cation and anion exchange chromatography, and agarose gel permeation chromatography and designated I, II, and III. The enzyme fractions appear analogous to the Class I, II, and III ribonucleic acid polymerases defined in other eucaryotic systems. The ionic strength optima, amatoxin sensitivity, and relative activities with denatured calf thymus deoxyribonucleic acid were different for Class I, II, and III polymerases.

A simple, rapid, reproducible method …

Surfactants As An Aid In The Kraft Pulping Of Hardwood, Bradley A. Veld

Paper Engineering Senior Theses

Surfactants were used in the pulping of Kraft hardwood chips in an attempt to decrease cooking time while attaining ·the same Kappa Number. It was found that surfactants could not be used to decrease cooking time, but anthraquinone and nonylphenol could be used to increase both yield and bond strength.Dimethylamides and quaternary ammonium salt surfactants were found to be detrimental to the pulping of hardwood resulting in hard uncooked chips

Ua77/1 Western Alumnus, Vol. 47, No. 4, Wku Alumni Relations

WKU Administration Documents

Quarterly magazine created for WKU alumni. Regular features are Hilltopics, Sports, Alumni Newsgrams and Notes and In Memoriam. This issue includes:

• Energy: Campus Conservation
• The Quilting Bee
• Old-Timers Take the Court
• Students and the Job Hunt
• Behind the Scenes
• Living and Learning Laboratories
• Rx for Ailing Indoor Plants
• Mardi Gras! ROTC on Parade
• Following the Yellow Brick Road
• Ford: Words of a President
• Photo Feature - President Downing
• Lights, Camera, Actioin!

Louisiana Agriculture Magazine, Spring 1977, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

Isolation, Purification, Scanning Electron Microscopy And Bacterial Dna Uptake Of Plant Protoplasts, Bronwyn G. Hughes

Theses and Dissertations

Protoplasts were isolated from tobacco and barley leaves in sucrose or mannitol using commercially available cellulases and macerozymes. Barley growth and protoplast isolation and purification conditions were optimized so that protoplasts were obtained in high yields free of unwanted debris and organelles. A technique for processing barley and tobacco protoplasts for examination by scanning electron microscopy was developed in which protoplasts seem to have maintained their structural integrity. Barley and tobacco protoplasts took up 3H-B. subtilis DNA, 125I-B. subtilis DNA or 125I-M. luteus DNA as a linear function of time (0-6 hr) and DNA concentration (0-200 μg/ml). Up to 16 …

A Bioassay Study Of Portage Creek Using Tetrahymena Pyriformis As An Indicator Organism, George T. Mimidis

Masters Theses

No abstract provided.

A Study Of Dietary Calcium And Phosphorus Intakes In Relation To Residual Ridge Resorption, Rita L. Sørensen

Loma Linda University Electronic Theses, Dissertations & Projects

Resorption of alveolar bone, a characteristic observed in periodontal disease, in the totally edentulous mouth and in generalized osteoporosis, is a common world-wide phenomenon in man. Calcium deficiency and calcium-phosphorus imbalance are implicated as plausible factors in all three instances.

A compilation of recent investigations strongly suggests that oral bone loss, whether in periodontal disease or in the residual ridges, is an early manifestation of osteoporosis; and that osteoporosis, in general, is associated with disturbed calcium homeostasis.

This study, patterned after that of Wical and Swoope, was conducted on 55 subjects who had been totally edentulous for at least three …

Apical Closure Induction Using Bone Growth Factors And Mineral Trioxide Aggregate, Kenneth W. Tittle

Loma Linda University Electronic Theses, Dissertations & Projects

This study compared the ability of three different bone growth factor combinations with that of Ca(OH)₂ to stimulate apical closure as well as the effectiveness of Mineral Trioxide Aggregate (MTA) as an apical barrier. Fifty-six roots of second and fourth mandibular premolars of seven six- month old beagle dogs were used. Periradicular lesions were created over a four week period. The canals were cleaned and shaped and filled with Ca(OH)₂ for one week. They were then irrigated and received one of seven different treatments in a balanced design. Growth factors were placed in a carboxymethylcellulose carrier and delivered …

Fine-Structure Map Of The Histidine Transport Genes In Salmonella Typhimurium, Giovanna Ferro-Luzzi Ames, K. Dale Noel, H. Taber, E. Negri Spudich, Kishiko Nikaido, J. Afong, F. Ardeshir

Biological Sciences Faculty Research and Publications

Afine-structure genetic map of the histidine transport region of the Salmonella typhimurium chromosome was constructed. Twenty-five deletion mutants were isolated and used for dividing the hisJ and hisP genes into 8 and 13 regions respectively. A total of 308 mutations, spontaneous and mutagen induced, have been placed in these regions by deletion mapping. The histidine transport operon is presumed to be constituted of genes dhuA, hisJ, and hisP, and the regulation of the hosP and hisJ genes by dhuA is discussed. The orientation of this operon relative to purF has been established by three-point crosses as being: purF …

Proceedings From The 14th Annual Marschall Invitational Italian Cheese Seminar 1977, Various Authors

Cheese Industry Conference

No abstract provided.

G77-355 A Guide For The Control Of Flies In Nebraska Feedlots And Dairies (Revised March 1990), John B. Campbell

University of Nebraska-Lincoln Extension: Historical Materials

Flies, especially stable and house flies, can create serious and costly problems for feedlot and dairy operations. This publication describes several methods for control.

Several species of flies may be in confined livestock facilities during summer. The stable and house fly are the most serious pests. Blow flies also may be present if molasses is in the diet. Horn flies--small blood-feeding flies--may be present in early spring. These flies overwinter as pupae in or near manure pats in range or pasture. If cattle are not present in the grassland when horn flies emerge, they will migrate to confinement cattle. Normally, …

Hemoglobin Alc: Structure, Biosynthesis And Clinical Significance In Diabetes Mellitus, Ronald J. Koenig

Student Theses and Dissertations

Diabetes mellitus is a chronic metabolic disorder characterized by abnormally high blood glucose concentrations and a relative or absolute deficiency of insulin. Approximately 5 per cent of the American population are afflicted with this disease, which is a leading cause of morbidity and mortality in the United States today. Diabetes may result in the dysfunction of many different organ systems, yet the biochemical mechanism(s) underlying these dysfunctions is unknown. Even the importance of hyperglycemia in the development of the sequelae of diabetes is unsettled. One of the metabolic abnormalities known to characterize this disease is an increase in the peripheral …

The Gross Morphology Of The Internal Tendons Of The Muscles Of The Human Lower Extremity, Nadhi Gagnantadilok

Dissertations

No abstract provided.

The Effects Of Enzymes On Activated Sludge Floc, Terry Lee Stoddart

Retrospective Theses and Dissertations

Dialysed activated sludge was used as a substrate to test for enzymes which can hydrolyse activated sludge floc. Two hundred and fifty aerobic and anaerobic microorganisms were tested against activated sludge for the presence of hydrolytic enzymes. These included known genera and organisms obtained by various enrichment procedures. Anaerobic digester mixed liquor was contacted with activated sludge agar under anaerobic conditions. None of the microorganisms present in the digester liquor hydrolysed the floc. The following commercial enzymes were contacted with activated sludge singly, in combination, and sequentially under various physical and chemical conditions: protease, lipase, cellulose, pectinase, phospholipase C, trypsin, …

G77-337 Propagating House Plants, Dale T. Lindgren, Don Steinegger

University of Nebraska-Lincoln Extension: Historical Materials

Propagating house plants in the home is an inexpensive and enjoyable hobby. The home is not always the ideal place, but most house plants can be propagated there satisfactorily with a minimum of special equipment.

Methods of Propagation

House plants may be propagated asexually, in which all new plants will be identical, in most cases, to the parent plant, or sexually, where the new plants will not necessarily be identical to the parent plants. Plants are propagated sexually by seeds. Cuttings, air-layering, division and runners are asexual methods of propagation.

Annual Report: 1977

Cherry County (Nebraska) Extension: Historical Documents

Foreward ..................... 1

Extension Organization and Planning ......................... 2-5

Reports of Accomplishments ......................... 6-14

Budget and Expenditures ......................... 15

Inventories ......................... 16-24

Vertical Drainage In Field Cores, Larry G. Wells, R. W. Skaggs

Biosystems and Agricultural Engineering Faculty Publications

No abstract provided.