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Bacteria In The Air Of Housed Swine Units, L.F. Elliot, T.M. Mccalla, J.A. Deshazer

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Two housed, swine-growing-finishing units were studied for numbers of total bacteria, fecal coliforms, Staphylococcus, and Salmonella in the air. At 30.5 and 122 cm from the floor, total colony-forming particles, as tested, averaged 3.4 x 10) and 1.3 x 105/m³ of air, respectively; whereas fecal coliform counts averaged 24 x 103 and 1.9 x 103/m³ of air, respectively. Only 41% of the organisms growing on Staphylococcus 110 medium tested as Staphylococcus. Of 458 Staphylococcus isolates, 5 were coagulase-positive. No Salmonella were detected in the air of the units tested.

A Comparison Of The In Vitro Phagocytic Activity Of Polymorphonuclear Leucocytes And Alveolar Macrophages From Man And Rabbits, Thomas S. Harrison

Biology ETDs

It has been thought for many years that the alveolar macrophage is the primary agent responsible for prevention or bacterial invasion or the lung. Numerous studies with Staphylococcus aureua, Staphylococcus epidermidis, Escherichia coli. Klebsiella, and Proteus have been cited to substantiate this statement. Together these organisms cause less than ten per cent of human bacterial pneumonias. Both in vivo and in vitro studies have shown that the alveolar macrophage is capable of bacterial clearance with time. Sadly lacking ss evidence that alveolar macrophages readily phagocytose Streptococcus pneumoniae which are responsible tor greater than ninety percent of all bacterial pneumonias. The …

The Isolation And Identification Of Yeast-Like Fungi In Non-Active Tuberculosis Patients In Rowan County, Kentucky, Connie Harris Baker

Morehead State Theses and Dissertations

A thesis submitted to Morehead State University in partial fulfillment of the requirements for the degree of Master of Science in Biology by Connie Harris Baker in July of 1976.

Constitutive Nature Of 5-Ketogluconate Reductase Of Gluconobacter Suboxydans, Vahag Marootkhanian

Masters Theses & Specialist Projects

NADP⁺-dependent 5-ketogluconate reductase (D-Gluconate:NADP⁺ oxidoreductase; E.C.I.I.I.69.) of Gluconoacter suboxydans was examined and determined to be constitutive in nature. Crude, cell -free enzyme extracts were prepared by sonication of cells grown on 2.0'% glucose and 2.0% ethanol in various experiments. The specific activity of 5-ketogluconate reductase in the crude enzyme extracts of cells grown under various conditions was determined. The specific activity remained relatively constant strongly indicating the constitutive nature of the enzyme.

Electrophoretic Heterogeneity Of Nicotinamide Adenine Dinucleotide Phosphate-Dependent 5-Ketogluconate Reductase Of Gluconobacter Suboxydans, Sharon Dianis

Masters Theses & Specialist Projects

NADP⁺-dependent 5-ketogluconate reductase (D-Gluconate:NAD(P)⁺ oxidoreductase E.C. 1.1.1.69) isozymes were detected in cell-free extracts of Gluconobacter suboxydans (American Type Cultures Collection, Bethesda, Maryland, strain 621). Cell-free enzyme extracts were prepared from cells grown in media which contained glucose, gluconate, glycerol, sorbitol, or mannitol as the sole carbon source in 2% concentration. The extracts were fractionated using analytical polyacrylamide disc-gel electrophoresis. Gels were stained specifically for 5-ketogluconate reductase. Three isozymes were present in each extract from cells grown on the five different carbon sources. Gels stained in a reaction mixture which contained glycerol exhibited two of the isozymes.

An …

Agricultural Experiment Station News July 1976

Agricultural Research Division: News and Annual Reports

CONTENTS:
FROM THE DIRECTOR'S DESK
PERSONNEL ACTIONS
GRANTS AND CONTRACTS
GENERAL NOTES
NEBRASKA AGRICULTURAL EXPERIMENT STATION PUBLICATIONS – June 1976
BULLETINS PRINTED

An Investigation Of The Nucleotide Cyclic 3'5' Adenosine Monophosphate In Young Hypocotyls Of Pisum Sativum L., Gary Marsh Hilton

Biological Sciences Theses & Dissertations

Concentrations of cAMP increased with time in pea hypocotyls assayed at 24 hour intervals over a period of four days. The highest concentration was 20.1 pmoles/mg protein which occurred in 96 hour hypocotyl tips. Ninety-six hour root bodies (dissected region between tip and embryo) achieved a concentration of 8.6 pmoles/mg. Concentrations of cAMP in embryos remained constant around one pmole/mg for three days and values doubled on the fourth day.

Hypocotyls, after germination for 72 hours, were dissected and incubated for various lengths of time in water and 3xl0^-8M IAA. Tips incubated for two hours showed a tenfold …

Productivity Of The Roll-Streak Method To Perform Anaerobic Bacteriology In The Routine Clinical Laboratory, Thomas R. Neblett

Henry Ford Hospital Medical Journal

Data presented are derived from one year of performing routine clinical anaerobic bacteriology by the roll-streak tube method. 826 specimens from 1508 (54.8%) contained anaerobes. 162 specimens were polymicrobic (19.6%) and contained 528 strains of a total of 1051 isolated (50.2 %). 479 blood specimens contained 525 strains of anaerobes. Propionibacterium, considered a contaminant, occurred 209/210 times in blood as the sole isolate. Bacteroides occurred alone or polymicrobically 308 times and comprised 54.7% of the blood isolates and 32.5% of the total anaerobic genera isolated. Clostridium, Peptococcus and Fusobacterium were the next most frequently occurring anaerobes. The roll-streak method is …

Distribution Of Stream Pollution In Lake Water, Richard W. Tew, Samuel S. Egdorf, James E. Deacon

Publications (WR)

Wastewater effluent-laden waters from Las Vegas Wash (LVW) form a density current that may be detected in Boulder Basin of Lake Mead at considerable distances from the wash estuary. This led to the suspicion that water from the inflowing stream [40 mgd (1.5 X 10⁵ cu m/day)] might not be rapidly diluted in the enormous volume of the lake [19 mil acre-ft (2.3 X 10¹⁰ cu m)], but might persist as a recognizable entity to the vicinity of the intake of a major water source for populous Clark County, Nev.

Because of the detection sensitivity implicit in the …

Evaluation F The Germ Tube Test & Other Tests For The Differentiation Of Candida Albicans From Candida Stellatoidea, Daniel Rogers

Masters Theses & Specialist Projects

Candida albicans is a pathogenic yeast often encountered in the medical laboratory. A survey taken by mail of 110 Kentucky hospital and clinical laboratories demonstrated that the germ tube test is the most frequently used method for the identification of this organism. Earlier workers have found that this technique does not distinguish between C. albicans and C. stellatoidea. Various modifications of the germ tube test that could possibly be used in the differentiation of these two taxa were investigated. Parameters examined included the effects of oxygen tension, CO₂, increased temperature, serum dilution, glucose concentration, and a synthetic …

The Role Of An Eyestalk Extract In The Regulation Of The Blood Sodium And Calcium Concentrations Of The Blue Crab Callinectes Sapidus, Gregory Martin Ivy

Master's Theses

The effect of bilateral eyestalk ligation or ablation on the blood Na+ and ca++ concentrations of the euryhaline blue crab, Callinectes sapidus, was measured in 10 o/oo and 5 o/oo sea water. The ablations caused a significant decrease in the blood Na+ and ca++ over a 24 hour period. The serum Na+ level increased significantly 6 hours following injections of an eyestalk extract whereas the blood total ca++ level did not change significantly. Eyestalk ligation, however, did not cause significant blood Na+ and ca++ changes. The blood Na+ level of intact crabs increased significantly in acclimating to colder water when …

Determination Of Lead Content In Apical Tissue Following Root Canal Obturation With Sargenti's Formula, G. Merle Hickok

Loma Linda University Electronic Theses, Dissertations & Projects

Four Stump-tail monkeys were used to help determine if the lead in Sargenti's formula diffuses out into the apical tissue while confined entirely within the root canal. In each animal two teeth were filled with Sargenti's formula 1-2 millimeters short of the radiographic apex, and two teeth were filled with gutta percha and Proco-Sol sealer for controls, making a total sample size of sixteen. The time ranged from 48 hours to 120 days. Lead analysis using the spectrophotometric method showed a higher lead content in the teeth filled with Sargenti's formula than with the gutta percha controls.

Role Theory Considered As An Influence On Criminal And Deviant Behavior In The Utah State Park System -- A Manager Problem, Gerald R. Grove Jr.

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Individual attitudes toward law enforcement and perceptions of camper role skills, along with a knowledge of park and campground rules, were measured with a questionnaire and compared through Partial Correlation and Pearson Product-Moment Correlations. Demographic information was also gathered and compared with the attitude and rules knowledge data for significance.

Results showed that attitude toward law enforcement and attitude toward camper role skills are significantly related. Attitude toward law enforcement and knowledge of rules also proved to have a significant relationship. However, attitude toward camper role skills had no statistical relationship to rules knowledge. The demographic data was found to …

Socioecology Of The Vicuna, William L. Franklin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The social organization of the vicuna (Vicugna vicugna) and its relationship to the environment were studied at the Pampa Galeras National Vicuna Reserve in southern Peru from May 1968 to April 1971. Behavioral data were collected on groups within a grid of 12.4 km² during 3,800 hours of observation. The vicuna's alpine grassland environment was characterized by greatly fluctuating and freezing temperatures, light precipitation, high evaporation, wind, short growing seasons, shallow and infertile soils and low plant production. Vicuna habitat quality was dependent upon high producing, preferred vegetation types and close access to permanent water and a …

Nitrogen Cycling In A Microcosm Simulation Of The Northern Arm Of The Great Salt Lake, John C. Stube

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Nitrogen cycling was studied in the hypersaline northern arm of the Great Salt Lake (332,480 mg/l total dissolved solids) using a sediment-water microcosm simulation of that system. The study demonstrated that nitrate, ammonia, and urea were not stimulating to the halophilic bacteria in the microcosm. The bacteria were simulated only indirectly simulated bacterial growth. In addition, no nitrification could be demonstrated during the study.

Weekly analyses of water samples from the north arm of the lake itself showed that nitrate and nitrite were absent, leaving ammonia as the only significant form of inorganic nitrogen in the northern lake basin. Other …

Abstracts Of Papers, Iowa Academy Of Science [88th Meeting], April 10, 1976, Iowa Academy Of Science

Iowa Academy of Science Documents

Presentation abstracts from the annual meeting of the Iowa Academy of Science

Ua12/2/19 Earth Week, Energy For Student Awareness

WKU Administration Documents

Newspaper created by the Energy for Student Awareness group regarding Earth Week and environmental issues.

The Nature And Distribution Of Enteric Bacteria In Las Vegas Bay, Samuel S. Egdorf

Publications (WR)

The distribution of water and of enteric bacteria of possible fecal origin into Las Vegas Bay from Las Vegas Wash was determined. Determination of distribution patterns was attained by applying the concept of the population component ratio of enteric bacteria. The development and application of new techniques and methods implicit in the application of the "component ratio" concept are discussed. The unreliability of thoroughly referenced techniques and methods generally accepted as standard are treated in detail. Physical factors affecting the distribution and deposition of enteric bacteria (including those of special public health importance) in Las Vegas Bay are also discussed.

Fixation Of Dormant Tilletia Teliospores For Thin Sectioning, John Scott Gardner

Theses and Dissertations

Dormant Tilletia caries teliospores in fixative solution or distilled water were frozen onto specimen chucks of an FTS Sorvall- Christensen frozen thin sectioner and cut or fractured at various temperatures (-20 to -75 C) and thickness settings (10, 15, 20, and 25 um). Cytoplasm of dormant spores was well preserved and organelles were found to differ from those of germinated spores in morphology. Irregular electron dense and electron opaque areas of lipid bodies were sometimes evident in dormant and germinating Tilletia caries teliospores. When dormant teliospores were sectioned in buffered fixative, lipids had an appearance similar to lipids in thin …

Test Variation In The Sand Dollar, Mellita Quinquiesperforata (Leske), George Glynn Rountree

OES Theses and Dissertations

Mellita quinguiesperforata is a common member of the benthos along the eastern coast of North America. This study considers variation of the test within the species, specifically: 1) variation in test weight, 2) variation in the length-width relationship, and 3) the average number of pore pairs in the ambulacral petals.

A total of 844 sand dollars from three localities was utilized: 1) Pass-a-Grill Beach, St. Petersburg, Florida,2) Bird Shoal, Beaufort, North Carolina, and 3) Fisherman Island, Virginia.

Animals were measured for length and width and the dry weight determined. Prediction equations for the size-weight relat1onships were computed for the three …

Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoretic Studies Demonstrating Cytoplasmic Protein Components Unique To The Mycelial Phase And To The Yeast Phase Of Candida Albicans, Theresa Catherine Burgess

Biological Sciences Theses & Dissertations

Blastospore and mycelial cytoplasmic extracts from four strains of Candida albicans, varying in virulence, were compared by sodium dodecyl sulfate (SDS) polyacrylamide disc gel electrophoresis Cytoplasmic extracts were made by disrupting the cultures in a Braun homogenizer followed by ultracentrifugation. The supernatant fractions were then resolved on polyacrylamide gels containing SDS. Each of the preparations yielded a distinct pattern. There were mycelial specific and blastospore specific protein bands. There were more components resolved from the blastospore phase cytoplasmic extracts than from the mycelial phase cytoplasmic extracts. There were bands common to both phases. These results demonstrate protein components unique …

Phytoseiid Predators Of Mite Pests In Utah Apple Orchards, Vichitra Mongkolprasith

Theses and Dissertations

Recent efforts to integrate certain Phytoseiid predators in pest (mite) management programs for apples have been somewhat successful and economical in many fruit growing areas. Convenient descriptions and reviews of the literature for species which have been collected from the state are provided to stimulate further work with Utah Phytoseiids. Seven species of Amblyseius and five Typhlodromus are described and notes provided concerning their respective geographic distributions and biologies.

"Nuclear Power: Its Role In Our Future", Betsy Macinness, Dave Ferg, Rudi Nussbaum, Tom Davis, Sandra Kiefer

Special Collections: Oregon Public Speakers

No abstract provided.

"Biological Determinism As A Social Weapon", Richard C. Lewontin

Special Collections: Oregon Public Speakers

No abstract provided.

Nitrogen Dynamics In Stands Dominated By Some Major Cool Desert Shrubs. V. Studies On Denitrification And Nitrogen Fixation: Comparison Of Biological Process In Western Deserts, J. J. Skujins

Memorandum

No abstract provided.

Columnaris Disease Of Fishes, S. F. Snieszko, G. L. Bullock

United States Fish and Wildlife Service: Publications

Columnaris disease is a chronic to acute infection that affects salmonids and many species of warmwater fishes. The first description of the disease was given by Davis (1922) who named the disease and bacterium from the columnar arrangement of cells as seen in wet mounts. The bacterium causing columnaris disease was first isolated by Ordal and Rucker (1944). They identified the organism as belonging to the group known as slime bacteria or myxobacteria; because it produced fruiting bodies and microcysts, they named it Chondrococcus columnaris. Garnjobst (1945), who was unable to find fruiting bodies, renamed it Cytophaga columnaris. …

Evaluation Of Critical Soil Properties Needed To Predict Soil Water Flow Under Desert Conditions, W. A. Jury, L. H. Stolzy, J. Letey Jr., L. V. Weeks

Memorandum

No abstract provided.

Farm, Ranch And Home Quarterly Institute Of Agriculture And Natural Resources, University Of Nebraska- Lincoln

Farm, Ranch and Home Quarterly

In this issue:

2 Scientists Trace Ancient Nitrogen in Deep Nebraska Soils

4 Backyard Farmer

5 Children's Imaginary Playmates

7 New Landscape Design Courses

9 New Wildflower Varieties Released

11 Fabric Softener Stains

12 4.-H'ers Become Trained Communication

14 A New Look at Animal Wastes as Fertilizer

16 Black Layer Signals Maturity

18 Roadside Hay Harvests

19 Protein and Lysine Enriched Bread

Limnology Of The Great Lakes Of Nicaragua, Gerald A. Cole

Investigations of the Ichthyofauna of Nicaraguan Lakes

For more than a century, the two largest bodies of water in Nicaragua, Lake Nicaragua and its smaller companion, Managua, have engaged naturalists from several disciplines. Perhaps their appeal has been as curiosities because of the occurrence of fish with marine affinities in the larger lake, especially a dangerous shark. The charm has been in Nicaragua or Cocibolca, rather than Managua, properly called Xolotlán. Even though the latter is saltier, it has no elasmobranchs or tarpons.
The lakes, and specifically the larger, cover the greatest expanse of any body of water between Lake Titicaca, Bolivia-Peru, and the Laurentian …

The Status Of The Lake Nicaragua Shark: An Updated Appraisal, Thomas B. Thorson

Investigations of the Ichthyofauna of Nicaraguan Lakes

Since 1966, an extensive tagging program has demonstrated beyond any further doubt (Thorson, 1971) that the sharks move from the Caribbean Sea to Lake Nicaragua and vice versa. At the time of this writing, of 1450 post-juvenile sharks tagged at the various river mouths on the Caribbean Coast, ten have been recovered in Lake Nicaragua; and of 146 tagged at San Carlos, where the river leaves the lake, 28 have been recovered along the Caribbean Coast, most of them at the various outlets of the Rio San Juan. Except for these basic facts, the results of the tagging program have …