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The Role Of Cuticular Strata Nomenclature In The Systematics Of Nemata, Armand R. Maggenti Jan 1979

The Role Of Cuticular Strata Nomenclature In The Systematics Of Nemata, Armand R. Maggenti

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A system of cuticular nomenclature based on the strata observed in Enoplia is proposed. Nematode cuticle is divided into four fundamental strata: epicuticle, exocuticle, mesocuticle, and endocuticle. Application of this system allows the correlation of complementary strata throughout Nemata. The major taxonomic categories within Nemata are differentiated on the basis of their cuticular strata as compared with the Enoplia model cuticle.


Review Of Fish Pathology, Glenn L. Hoffman Jan 1979

Review Of Fish Pathology, Glenn L. Hoffman

United States Fish and Wildlife Service: Publications

This excellent, somewhat Scottish text (the editor and most contributors live in Scotland), consists of 14 well-illustrated and informative chapters: 1. The Aquatic Environment by A. L. S. Munro; 2. The Anatomy and Physiology of Teleosts by A. E. Ellis, R. J. Roberts, and P. Tytler; 3. The Pathophysiology and Systematic Pathology of Teleosts by R. J. Roberts; 4. The Immunology of Teleosts by A. E. Ellis; 5. Neoplasia of Teleosts by Joan Budd and R. J. Roberts; 6. The Virology of Teleosts by Janet Liversidge and A. L. S. Munro; 7. The Parasitology of Teleosts by Ted Needham and …


Coccidian Parasites Of Wild Turkeys, Meleagris Gallopavo Silvestris, In Illinois, D. Michael Watkins Jan 1979

Coccidian Parasites Of Wild Turkeys, Meleagris Gallopavo Silvestris, In Illinois, D. Michael Watkins

Masters Theses

Fecal samples from 124 wild turkeys (Meleagris gallopavo silvestris), collected in three southern Illinois counties, were examined for coccidia. Three and two tenths percent (4) of the birds sampled were infected with Eimeria of apparently four different species. These species were tentatively designated as: E. meleagridis, E. adendeides, E. meleagrimitis and E. subrotunda.


In Vitro And Clinical Studies Of Cefaclor, A New Cephalosporin, Daria Kiani, Tom Madhavan, Keith Burch, Don Pohlod, Evelyn Fisher, Edward L. Quinn Dec 1978

In Vitro And Clinical Studies Of Cefaclor, A New Cephalosporin, Daria Kiani, Tom Madhavan, Keith Burch, Don Pohlod, Evelyn Fisher, Edward L. Quinn

Henry Ford Hospital Medical Journal

In vitro susceptibility studies of 246 clinical isolates demonstrated that this antibiotic was effective against Group A beta hemolytic streptococci, alpha hemolytic streptococci, S. pneumoniae, both penicillinase producing and nonpenicillinase producing Staphylococci, Klebsiella pneumoniae, and Proteus mirabilis. Cephalothin susceptible E. coii were also susceptible to cefaclor. Pseudomonas aeruginosa, Serratia sp., Enterobacter Sp., and Streptococcus faecalis were uniformly resistant to cefaclor. The efficacy and safety of this antibiotic were studied in 27 patients with urinary tract, soft tissue, and respiratory infections. Patients with urinary tract infections became abacteriuric after 48 hours. Patients with soft tissue infections responded well within the first …


Review Of Parasitic Protozoa. Volume Iii: Gregarines, Haemogregarines, Coccidia, Plasmodia, And Haemoproteids. Edited By Julius P. Kreier (Academic Press, 1977), Donald W. Duszynski Dec 1978

Review Of Parasitic Protozoa. Volume Iii: Gregarines, Haemogregarines, Coccidia, Plasmodia, And Haemoproteids. Edited By Julius P. Kreier (Academic Press, 1977), Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Review of Parasitic Protozoa. Volume III: Gregarines, Haemogregarines, Coccidia, Plasmodia, and Haemoproteids. Edited by Julius P. Kreier. New York : Academic Press, 1977.


A Study Of Lymphocyte Subpopulations In Allergic And Asthmatic Patients, Cleora W. Barnwell Oct 1978

A Study Of Lymphocyte Subpopulations In Allergic And Asthmatic Patients, Cleora W. Barnwell

Retrospective Theses and Dissertations

The role of T and B lymphocytes in the human immune response has been studied in allergic and asthmatic patients by many investigators. Theories of suppressed cellular immunity have been reported showing decreased levels of T cells in peripheral blood. Elevated levels of IgE, an antibody, have been reported in these conditions, but not shown to be significant in distinguishing the different types of asthma. A double blind study of 19 allergic and 42 asthmatic individuals was conducted measuring T and B cell levels and IgE levels. The asthmatic group was subdivided into three types, intrinsic, extrinsic and mixed. The …


Two New Species Of Acanthobothrium Van Beneden 1849 (Cestoidea: Tetraphyllidea) From Freshwater Stingrays In South America, Monte A. Mayes, Daniel R. Brooks, Thomas B. Thorson Oct 1978

Two New Species Of Acanthobothrium Van Beneden 1849 (Cestoidea: Tetraphyllidea) From Freshwater Stingrays In South America, Monte A. Mayes, Daniel R. Brooks, Thomas B. Thorson

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Two new species of Acanthobothrium are reported from freshwater stingrays in northern South America. Acanthobothrium quinonesi sp. n. is described from Potamotrygon magdalenae Dumeril collected from the Magdalena River in northern Colombia and A. amazonensis sp. n. from P. circularis Garman collected in the Itacuai River of northwestern Brazil. Acanthobothrium quinonesi differs from A. amazonensis by having a smaller and anteriorly-curved cirrus sac, 43-60 rather than 50-72 testes, and bothridial hooks 100-142 μm long rather than 145-184 μm long. Characters used in the taxonomy of Acanthobothrium species are listed to show that the new species resemble each other and A. …


Isolation And Chemico-Physiologic Characterization Of A Parotid Hormone Activity From Porcine Glands, Jean-Marc Tieche Sep 1978

Isolation And Chemico-Physiologic Characterization Of A Parotid Hormone Activity From Porcine Glands, Jean-Marc Tieche

Loma Linda University Electronic Theses, Dissertations & Projects

For the past decade, evidence has accumulated which suggests that the resistance of the teeth to dental decay is in part under systemic control. It was shown that the resistance is accomplished through the optimal operation of a dentinal fluid transport mechanism which is believed to be hormonally controlled by the hypothalamic-parotid gland endocrine axis. Although circumstantial evidence for such an endocrine function was strong, the isolation of a parotid hormone which stimulates the dentinal fluid transport mechanism remained to be accomplished.

Using modern biochemical techniques, a methodology was designed to isolate and purify the hormone from porcine parotid glands. …


Experimental Transmission Of Sarcocystis From Icterid Birds To Sparrows And Canaries By Sporocysts From The Opossum, Edith D. Box, Donald W. Duszynski Aug 1978

Experimental Transmission Of Sarcocystis From Icterid Birds To Sparrows And Canaries By Sporocysts From The Opossum, Edith D. Box, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Cowbirds (Molothrus ater) and grackles (Cassidix mexicanus) infected with muscle cysts of Sarcocystis were fed to opposums (Didelphis virginiana) and fecal sporocysts from the latter were given to sparrows (Passer domesticus, Family Ploceidae), canaries (Serinus canarius, Family Fringillidae) and ducks (Anas platyrhynchos, Family Anatidae). Asexual parasites were found in the endothelium of sparrows and canaries but not in ducks. When birds were kept 10 weeks or more after infection, muscle cysts were found grossly and microscopically in the majority of sparrows, and in 1 canary, but not in …


Acanthobothrium Electricolum Sp. N. And A. Lintoni Goldstein, Henson, And Schlicht 1969 (Cestoda: Tetraphyllidea) From Narcine Brasiliensis (Olfers) (Chondrichthyes: Torpedinidae) In Colombia, Daniel R. Brooks, Monte A. Mayes Aug 1978

Acanthobothrium Electricolum Sp. N. And A. Lintoni Goldstein, Henson, And Schlicht 1969 (Cestoda: Tetraphyllidea) From Narcine Brasiliensis (Olfers) (Chondrichthyes: Torpedinidae) In Colombia, Daniel R. Brooks, Monte A. Mayes

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Acanthobothrium lintoni and a new species of Acanthobothrium are reported from the lesser electric ray Narcine brasiliensis in Colombia. The new species resembles all other members of the genus parasitizing torpedinids by having a relatively small scolex, long and spinose cephalic peduncle, fewer than 75 testes per proglottid, an indistinct genital atrium, and V-shaped ovaries with ovarian lobes of equal length which do not reach the posterior margin of the cirrus sac. The new species differs from all known species by having bothridial hooks only 46 to 64 μm in total length. A key to the species of Acanthobothrium parasitizing …


Effects Of Trypanosoma Musculi Kendall, 1906 Immune Serum On The Reproduction Of Trichinella Spiralis (Owen, 1835) Railliet, 1896 In Mice, Samuel L. Chafin Jun 1978

Effects Of Trypanosoma Musculi Kendall, 1906 Immune Serum On The Reproduction Of Trichinella Spiralis (Owen, 1835) Railliet, 1896 In Mice, Samuel L. Chafin

Loma Linda University Electronic Theses, Dissertations & Projects

The possible effects of ablastin, a reproduction-inhibiting antibody which is produced during the infection of the blood parasite, Trypanosoma musculi in the mouse, were investigated by fractionating the parasite into a supernatant fraction (designated as Group A test mice) and a pellet fraction (Group B test mice). These groups A and B mice were injected with the respective fractions, plus Freund’s Complete Adjuvant (FCA). Control mice were injected with FCA only. Sera from these three groups of mice were removed after the Group A and Group B mice were shown to be negative to T. musculi challenge, and Group A …


Adult Cestodes From The Coelomic Cavity Of The Teid Lizard, Cnemidophorus Sexlineatus, Wesley L. Shoop, John J. Janovy Jr. Jun 1978

Adult Cestodes From The Coelomic Cavity Of The Teid Lizard, Cnemidophorus Sexlineatus, Wesley L. Shoop, John J. Janovy Jr.

John J. Janovy, Jr. Publications

In a survey of 64 Cnemidophorus sexlineatus, adult cestodes identified as Oochoristica bivitellobata with gravid proglottids were found in the coelomic cavity of two hand caught specimens at Cedar Point Biological Station, Ogallala, Nebraska, during June and July 1977. Two O. bivitellobata were found free in the coelom of a male lizard, whereas four were found in the coelom and one in the intestine of a female. No ruptures were found in the walls of the intestines of all lizards examined. In addition, 61 O. bivitellobata were found in the intestine of nine (14%) of the C. sexlineatus with …


Effect Of Time And Diet On The Immune Status Of Mice Challenged With Herpes-Transformed Cells, Daila S. Gridley May 1978

Effect Of Time And Diet On The Immune Status Of Mice Challenged With Herpes-Transformed Cells, Daila S. Gridley

Loma Linda University Electronic Theses, Dissertations & Projects

Human Herpes simplex virus type 2 (HSV 2)-transformed Balb/c mouse cells (H238 cells) are malignant in Balb/c mice. The immune stratus of mice bearing H238 cell-induced tumors was studied and changes in immune response with time and with altered diet of the mice were measured.

In the experiments dealing with the status of immunity with time after tumor cell injection, mice were fed Purina Laboratory Chow (P.L.C.), injected with H238 cells, and sacrificed three to eight weeks post-injection. In the diet experiments, groups of mice were fed either P.L.C. or one of six equicaloric diets, consisting of low or high …


The Wavelength Dependence Of Ultraviolet Enhanced Reactivation In A Mammalian Cell-Virus System, Leslie C. James Apr 1978

The Wavelength Dependence Of Ultraviolet Enhanced Reactivation In A Mammalian Cell-Virus System, Leslie C. James

Masters Theses & Specialist Projects

The effect of ultraviolet (UV) radiation in the wavelength region 230 nm to 302 nm on the ability of an irradiated mammalian cell to reactivate UV-irradiated mammalian virus was tests. An action spectrum for radiation enhanced reactivation (RER) Is presented. The shape of the action spectrum points to a combined nucleic acid protein target for UV radiation effects on this cellular parameter. An analysis of the results of others involving the biochemical and photobiological events involved in the RER does not allow the identification of the macromolecule which is the major contributor to this effect. Studies involving an analogous phenomenon …


Spirocamallanus Cricotus Sp. N. And S. Halitrophus Sp. N. (Nematoda: Camallanidea) From Fishes In The Northern Gulf Of Mexico, Alan C. Fusco, Robin M. Overstreet Apr 1978

Spirocamallanus Cricotus Sp. N. And S. Halitrophus Sp. N. (Nematoda: Camallanidea) From Fishes In The Northern Gulf Of Mexico, Alan C. Fusco, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Spirocamallanus cricotus sp. n. (= S. pereirai, in part) and S. halitrophus sp. n. are described from marine fishes of the northern Gull of Mexico. Spirocamallanus cricotus has a ledge anterior to the basal ring in the buccal capsule, similar spicules with a ratio of 1:1.4 to 2.1, 3 pre- and 5 postcloacal papillae, and 8 rectal glands in the female; S. halitrophus lacks the ledge and possesses dissimilar spicules with a ratio of 1:1.3 to 1.8, 3 pre- and 6 postcloacal papillae, and 4 rectal glands in the female.


The Opossum (Didelphis Virginiana) As A Host For Sarcocystis Debonei From Cowbirds (Molothrus Ater) And Grackles (Cassidix Mexicanus, Quiscalus Quiscula), Donald Duszynski, Edith D. Box Apr 1978

The Opossum (Didelphis Virginiana) As A Host For Sarcocystis Debonei From Cowbirds (Molothrus Ater) And Grackles (Cassidix Mexicanus, Quiscalus Quiscula), Donald Duszynski, Edith D. Box

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Sarcocystis-infected muscles from ducks, cowbirds, and grackles were fed to cats, opossums, rats, and a dog. Only the opossum (Didelphis virginiana) was a suitable definitive host. All opossums that were fed Sarcocystis-infected cowbirds (Molothrus ater) and grackles (Cassidix mexicanus and Quiscalus quiscula) passed sporocysts in their feces. Opossums that ate the cowbirds had prepatent periods of 5 and 10 days and remained patent for at least 105 days. Opossums that ate the grackles became patent on day 10 after the infective meal and remained patent for over 90 (Quiscalus) and …


Simple Procedure For Disruption Of Fungal Spores, James L. Van Etten, Shelby Freer Mar 1978

Simple Procedure For Disruption Of Fungal Spores, James L. Van Etten, Shelby Freer

Department of Plant Pathology: Faculty Publications

A simple, inexpensive method for disrupting dormant fungal spores is described.


Implication Of An Environmentally Induced Hamartoma In Commercial Shrimps, Robin M. Overstreet, Thomas Van Devender Mar 1978

Implication Of An Environmentally Induced Hamartoma In Commercial Shrimps, Robin M. Overstreet, Thomas Van Devender

Harold W. Manter Laboratory of Parasitology: Faculty Publications

An overgrowth of muscle protruded through the ventral portion of the sixth abdominal segment of Penaeus aztecus and P. setiferus. This hamartoma was observed in 33 postlarval shrimp and is the first reported from a crustacean. Even though afflicted individuals represented a small proportion of the shrimp examined, nearly all such individuals were collected from the presumed most heavily polluted site. We suggest an unidentified pollutant as the cause of the abnormality.


Permanent Light Microscopy Slides Of Eimeria Nieschulzi Oocysts, Alan A. Marchiondo, Donald W. Duszynski Feb 1978

Permanent Light Microscopy Slides Of Eimeria Nieschulzi Oocysts, Alan A. Marchiondo, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The methods described here allow for the preparation of light microscopy slides of coccidian oocysts and thus, provide a durable sample and record for research and teaching. These methods used only one species, Eimeria nieschulzi; however, it is likely that use of other coccidian oocysts would produce similar results.


G78-417 Leptospirosis Of Domestic Animals, Donald B. Hudson Jan 1978

G78-417 Leptospirosis Of Domestic Animals, Donald B. Hudson

University of Nebraska-Lincoln Extension: Historical Materials

Leptospirosis of domestic animals is a very complex disease. This NebGuide examines its diagnosis, treatment, and prevention.

Introduction

Leptospirosis is an infectious disease of animals and man caused by a spiral-shaped organism (spirochete) of the genus Leptospira. The important serotypes recognized in livestock in the United States include Leptospira pomona, L. canicola, L. icterohaemorrhagiae, L. grippotyphosa and L. hardjo. These organisms have a wide host range, including man. Among domestic animals, swine, cattle, dogs, and horses are most frequently affected. Known wildlife hosts include many of the small rodents, raccoons, foxes, opossums, skunks, deer, and moose.

Because of the …


New Policy Hits Hard At Sheep Lice, F C. Wilkinson Jan 1978

New Policy Hits Hard At Sheep Lice, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

In March 1977 the regulation requiring compulsory routine dipping of all sheep within 30 days of shearing changed, and only infested flocks were required to be dipped. This change has allowed concentrated eradication efforts.


Reasons Why Cows Were Removed From The Sdsu Fairy Herd 1. Discriminant Analysis To Classify Cows With Or Without Reproductive Problems, Fred Tidemann Jan 1978

Reasons Why Cows Were Removed From The Sdsu Fairy Herd 1. Discriminant Analysis To Classify Cows With Or Without Reproductive Problems, Fred Tidemann

Electronic Theses and Dissertations

One of the keys to financial success in the dairy business is the ability of management to detect herd problems and correct them instead of culling cows. It is well known that the average productive life of the dairy cow is less than 4 yr, which is short compared with her potential life. Involuntary removal of cows causes economic loss directly as a result of its effect on yearly milk production, increased replacement cost, and indirectly because the potential selection differential is reduced with premature loss of high producing cows. The degree of culling is related to important economic considerations …


Prehistoric Cremations From Nogales, Arizona *, Karl J. Reinhard, Jeff H. Shipman Jan 1978

Prehistoric Cremations From Nogales, Arizona *, Karl J. Reinhard, Jeff H. Shipman

Karl Reinhard Publications

In October, 1969, the Highway Salvage Section of the Arizona State Museum conducted emergency salvage excavations in conjunction with the construction of the Tucson-Nogales Highway. Ten cremations were recovered from a backhoe trench which had been placed within the city limits of Nogales, Arizona. Analysis of the cremations indicated cultural contact between the Trincheras culture of Sonora, Mexico, and the Hohokam culture of the Santa Cruz River Valley in southern Arizona.


Food Of The Atlantic Croaker, Micropogonias Undulatus, From Mississippi Sound And The Gulf Of Mexico, Robin M. Overstreet, Richard W. Heard Jan 1978

Food Of The Atlantic Croaker, Micropogonias Undulatus, From Mississippi Sound And The Gulf Of Mexico, Robin M. Overstreet, Richard W. Heard

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The diet of the Atlantic croaker from Mississippi Sound has been examined for the first time. Over 83 taxa were encountered, or more than were reported from croaker in any other region. We also found 60 taxa, 36 of which overlapped with the above, in croaker from various offshore stations in the Gulf of Mexico. In Mississippi Sound the frequency of occurrcnce of items revealed primarily crustaceans followed by polychaetes, molluscs, fishes, and less common items, and, in the open Gulf, molluscs appeared slightly more often than in inshore croaker and than polychaetes in offshore fish. The diets were assessed …


Food Of The Red Drum, Sciaenops Ocellata, From Mississippi Sound, Robin M. Overstreet, Richard W. Heard Jan 1978

Food Of The Red Drum, Sciaenops Ocellata, From Mississippi Sound, Robin M. Overstreet, Richard W. Heard

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Examined digestive tracts of the red drum in Mississippi Sound contained mostly decapod crustaceans. Crustaceans accounted for 34 of 59 encountered taxa, more than reported from any other region. Nevertheless, the general diet for 104 fish with food contents out of the 107 examined is similar to that reported for red drum in several other studies from other areas. In addition to crustaceans, fishes followed by polychaetes occurred as the most important items (in 99, 43, and 15% of the drum with food, respectively). Blue crabs occurred in even more drum than the frequently encountered penaeid shrimps. Other commercial species …


Suppressed Rejection Of Trichinella Spiralis In Immunized Rats Concurrently Infected With Eimeria Nieschulzi, Donald W. Duszynski, Deborah Russell, Shirley A. Roy, Gilbert A. Castro Jan 1978

Suppressed Rejection Of Trichinella Spiralis In Immunized Rats Concurrently Infected With Eimeria Nieschulzi, Donald W. Duszynski, Deborah Russell, Shirley A. Roy, Gilbert A. Castro

Harold W. Manter Laboratory of Parasitology: Faculty Publications

This study deals with the effect of Eimeria nieschulzi infection on the host immune response to Trichinella spiralis. Six male Sprague-Dawley rats were inoculated with 104 and six with 106; sporulated E. nieschulzi oocysts. On days 2, 8, and 16 post-inoculation (PI), two rats from each infected group, and their paired uninfected controls, were killed and mucosal scrapings from their small intestines were assayed for peroxidase activity. Peroxidase levels were higher than corresponding control values on days 2 and 16 PI and significantly lower than the controls on day 8 PI. These data led us to …


Capillaria Sp. From Cryptotis Parva, Joseph N'Dong Jan 1978

Capillaria Sp. From Cryptotis Parva, Joseph N'Dong

Masters Theses

Twenty-nine Cryptotis parva were trapped at Lincoln Log Cabin State Park in Coles County Illinois between 22 December 1976 and 31 January 1978. Trapped specimens were necropsied and examined for the presence of helminth parasites. Nematode infections were found to be present in eight (27.2%) of the shrews collected with five (17.2%) of the males and three (10.3%) of the females infected. Three adult female nematodes were recovered from the small intestines of the host specimens, described and identified as the genus Capillaria. No male Capillaria were obtained and therefore species identification was impossible. However, the general appearance of …


Epizoic Protozoa Of Planktonic Copepoda And Cladocera From A Small Eutrophic Lake, And Their Possible Use As Indicators Of Organic Water Pollution, Michael Stevens Henebry Jan 1978

Epizoic Protozoa Of Planktonic Copepoda And Cladocera From A Small Eutrophic Lake, And Their Possible Use As Indicators Of Organic Water Pollution, Michael Stevens Henebry

Masters Theses

The epizoic protozoa of planktonic copepoda and cladocera from a eutrophic lake (Ashmore Lake, Coles County, Illinois) were studied over a 13-week period in the summer and fall of 1975. All organisms were collected with a 125 micron mesh plankton net. Epizoans (all peritrichs and suctorians) were hosted by three of four species of copepods and four of the ten species of cladocerans. The percentage of total organisms hosting epizoans varied significantly (0.001 alpha level) by month, but averaged 11.6 percent. This high occurrence of epizoans was thought to be related to the highly-eutrophic conditions existing in the lake. If …


Biochemical And Antibiogram Characteristics Of Certain Enterobacteriaceae, John Shaffer Jan 1978

Biochemical And Antibiogram Characteristics Of Certain Enterobacteriaceae, John Shaffer

University of the Pacific Theses and Dissertations

This study is a continuation of the investigations of Hall (1976), Storey (1977), and Berkowitz (1978) on antimicrobial susceptibility patterns of bacteria isolated from local hospitals in Stockton, California. It extends the work of Hall (1976) on the K-E-S group to include three species of Proteus and Escherichia coli. New antibiotics (tobramycin, amikacin and cefamandole) are also added to the antibiogram.


Legionnaires' Disease: Description Of A Sporadic Case, Louis D. Saravolatz, Gene Tilchen Dec 1977

Legionnaires' Disease: Description Of A Sporadic Case, Louis D. Saravolatz, Gene Tilchen

Henry Ford Hospital Medical Journal

This first case of Legionnaires' disease seen at Henry Ford Hospital is described. A previously healthy 72-year-old man developed progressive pneumonia in September, 1977; it was unresponsive to treatment with cephalothin. However, he rapidly recovered when the treatment was changed to oral erythromycin. Diagnosis was established by serologic studies done at the Center for Disease Control. This pneumonia occurred in a sporadic form with no evidence of secondary cases.