Digital Commons Network^™

The Effect Of Escherichia Coli Endotoxin On The Cat Periapical Tissues, Thomas G. Dwyer

Loma Linda University Electronic Theses, Dissertations & Projects

The periapical tissue reaction to three concentrations of endotoxin solutions and to a sterile saline control solution was examined in six adult cats. The maxillary and mandibular cuspids were isolated and the pulps extirpated with barbed broaches. The four solutions (1, 10, 100 μg/ml endotoxin, and saline) were then injected into the four prepared teeth for each cat and the access cavities sealed with IRM.

At two week intervals, two cats were sacrificed and the specimens removed, while the remaining animals were retreated as previously described. The block tissue sections were radiographed, fixed in 10% buffered formalin, and prepared for …

Concomitant Infections With The Parasites Trypanosoma Musculi Kendall, 1906 And Trichinella Spiralis (Owen, 1835) Raillet, 1896 In The Laboratory Mouse, Musmusculus, Joan Chew Liang Tan

Loma Linda University Electronic Theses, Dissertations & Projects

Four groups of female Swiss Webster mice were injected intraperitoneally with similar volumes of normal saline, sonicated Trypanosoma musculi and saline, sonicated trypanosomes and Freund's Complete Adjuvant (FCA) and FCA. When the mice became immune and thus responded negatively to a T. musculi challenge, sera were collected and administered to four other groups of mice to establish passive immunity in these animals.

An established dose of innoculum of Trichinella spiralis larvae (8.1 larvae/gram of body weight) was given to all the mice by gastric gavage. These mice were killed 26 days later and the musculature digested with artificial gastric juice …

The Effects Of Temperature And Ph On The Differentiation Of Trypanosoma Lewisi (Kent, 1880) Laveran & Mesnil, 1901, In A Defined Medium, Carolann R. Rosario

Loma Linda University Electronic Theses, Dissertations & Projects

Trypanosoma lewisi is an arthropod-transmitted protozoan parasite found in the bloodstream of rats. In its life cycle it undergoes cellular differentiation. It is primarily found as the trypomastigote form in the rat and as the epimastigote form in the arthropod, the rat flea. Various studies have been done on hemoflagellates to determine the factors responsible for stimulating the differentiation from one form to the other. Included in these factors are pH, temperature, and protein fractions from fetal calf serum. Recent efforts have also been aimed at developing simple or defined media for physiological or biochemical studies on hemoflagellates. The purpose …

Alveolar Hydatid Disease In Minnesota: First Human Case Acquired In The Contiguous United States, William G. Gamble, Martin Segal, Peter M. Schantz, Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A 56-year-old woman from southwestern Minnesota underwent an extended left hepatic lobectomy to remove a large multinodular mass with a necrotic central cavity. The clinical, serological, and pathological findings led to the diagnosis of alveolar hydatid disease, and specific identification of Echinococcus multilocularis was achieved by growing mature larvas in voles inoculated intraperitoneally with tissue from the hepatic lesions. The patient probably acquired her infection some years previously from pet cats or dogs that had become infected by ingesting infected rodents. In North America E. multilocularis is enzootic in the northern tundra zone of Alaska and Canada. Since 1964 the …

A Survey Of Joint Disease At The Libben Site, Ottawa County, Ohio, Marc A. Kelley

Henry Ford Hospital Medical Journal

No abstract provided.

Nueva Especie Del Género Acanthobothrium Van Benedem 1849; Cestode: Tetraphyllidea De Myliobatis Peruvianus Garman 1913, Ruperto Severino L., Luz Sarmiento B.

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Se describe Acanthobothrium gonzalesmugaburoi n.sp. (Cestoda: Tetraphyllidea: Onchobotriidae) hallado en la válvula espiral de 10 ejemplares de "raya aguila" Myliobatis peruvianus Garman, 1913, procedentes del litoral peruano. Difiere de las demás especies del género, por el número y distribución de los testículos y por la longitud total del gancho. Huésped y localidad se señalan por primera vez.

Ryegrass Toxicity Organism Found On Other Grasses, D L. Chatel, J. L. Wise, A. G. Marfleet

Journal of the Department of Agriculture, Western Australia, Series 4

Yellow slime disease caused by Corynebacterium sp. has recently been found in three grass species.

The diseased grasses were found in the field growing with each other and with affected toxic annual ryegrass.

Chromosomal Replicative Forms Of P1 And P1dlac During The Lytic Cycle, John W. Bornhoeft

Dissertations

No abstract provided.

Effect Of Traumatic Injury On Sensitivity To Insulin, Karl M. Nelson

Dissertations

No abstract provided.

Feeding And Housing Of Dairy Calves, Lester Vance Schaffer

Electronic Theses and Dissertations

In an 18 mo study antibiotic containing mastitic milk was collected from infected cows and fermented naturally for a minimum of 7 days at ambient temperature. Fermentation of antibiotic containing mastitic milk increased titratable acidity from 0.209% to 0.650% and decreased pH from 7.02 to 5.48. Fermentation decreased crude protein from 3.7% to 3.5% and increased NPN (% TN) from 6.0% to 8.4%. Penicillin was detected at a concentration of 1.2 IU/ml in the first milk after treatment and 0.01 IU/ml when fermented. Species of pathogenic organisms isolated from fresh and fermented milk were Staphylococcus epidermidis, Escherichia coli, Streptococcus dysgalactiae, …

Notes On Seasonal Variation And Infectivity Of Haemogamasus Reidi, A Vector Of Hepatozoon Griseisciuri, On Eastern Gray Squirrels, Sciurus Carolinensis, Mohammed Bashiru

Masters Theses

Five hundred and twenty-two squirrels were trapped and retrapped from a population in Montgomery County, Maryland during 1974-1975. One hundred and forty-one squirrels were found to harbor an average of 10.6 mites (Range 175-1). While male squirrels outnumbered females (79 vs 62) females harbored more mites than males (829 vs 666). During the spring the average number of mites per captured squirrel increased to an average of 18.1 per squirrel. The average declined to 6.8, 4.8 and 3.3 per squirrel in summer, fall and winter respectively. Throughout the collection period adult female mites were by far more numerous on hosts …

Hei-Gro Intravaginal Devices And Synovex-H Implants For Feedlot Heifers, James P. Goodman

Electronic Theses and Dissertations

Heifers gain at a slower rate, generally require more feed per unit gain and sell at a lower market price than steers. Due to the fact that heifer carcasses are commonly fatter and contain more waste trim than steers, the value of a heifer carcass is routinely less than that of a steer carcass of comparable yield and quality grade. In the past, the ways in which the livestock operator could increase his profit from feeding heifers were limited. Differential cost of feeder heifers versus steers and feed cost were the major criteria involved in his decision of whether or …

Role Of A Nonimmunoglobulin Cell Surface Determinant In The Activation Of B Lymphocytes By Thymus-Independent Antigens*, Subbarao Bondada, Donald E. Mosier, Aftab Ahmed, James J. Mond, Irwin Scher, William E. Paul

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Lyb 5 is a B-cell alloantigen which is expressed on 50-60% of B cells. It was defined originally on the basis of cytotoxicity. We have described a new reactivity within the anti-Lyb 5 serum on the basis of selective inhibition of antibody responses in vitro by this antiserum in the absence of complement. This inhibitory activity of anti-Lyb 5.1 serum appears to be due to recognition of antigenic determinants different from the prototype antigens detected in the cytotoxicity assay. Anti-Lyb 5 serum incorporated into spleen cell cultures selectively inhibits antibody responses to a class of thymus-independent antigens (TI-2) previously characterized …

Tritium (3h) Radiolabeling Of Protein A And Antibody To High Specific Activity: Application To Cell Surface Antigen Radioimmunoassays, Ronald L. Wilder, Catherine C. Yuen, Subbarao Bondada, Virgil L. Woods, Cornelius B. Alexander, Rose G. Mage

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Staphylococcal protein A and several different immunoglobulins have been radio-labeled to high specific activities (> 106 cpm/pg) by reductive methylation with tritiated (3H) sodium borohydride. The proteins retain excellent functional and antigenic properties. The utility of these reagents in a variety of assays for cell surface antigens is illustrated. The results indicate that this radiolabeling procedure may become the method of choice for many cell surface and solution immunoassays.

The Feasibility Of Detecting Poliovirus I In Water By Radioimmunoassay, Sheril K. Charba

Retrospective Theses and Dissertations

A microtiter, solid-phase, indirect radioimmunoassay (RIA) has been developed and evaluated as a method for detecting poliovirus in water samples. Antiserum to poliovirus Type I, LSc2ab, was prepared in rabbits, and high titer, high avidity antiserum to rabbit globulins were radio- actively labeled with 125I by a modification of the Bolton-Hunter and chloramine T methods to increasing specific activities. The immunoreactivity of the labeled antibodies was assessed. After the preparation and standardization of all components of the assay. The optimum assay conditions were determined. These conditions included the method of coating the microtiter wells with antigen, the time and temperature …

Flystrike In Sheep : Secondary And Tertiary Flies Striking Sheep In Western Australia, N Monzu

Journal of the Department of Agriculture, Western Australia, Series 4

Unlike primary species of blowflies, the secondary and tertiary species of flies cannot initiate a strike, but their activity in the presence of the primary blowflies becomes significant in the death of struck sheep. This is particularily so when the hairy maggot blowfly is involved. Future management of sheep flocks to minimise deaths may require recognition of these secondary and tirtiary flies.

This article presents some basic facts about these flies.

Saving The State From Rabbits, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

A thoughtful look at the rise and fall of rabbit populations in Western Astralia and the work done to achive the current levels.

Humanities In Medical Education- The Past Ten Years, James J. Quinn

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

What has always fascinated me is an historical account that ends triumphantly but whose start-to-finish is riddled with reversals and hostile obstacles and haunted by anguishing circumstances. The history of "humanities in medical education- the past ten years" in the United States is just such a story. In the last ten years there has been a continually wider acceptance of humanities in United States medical schools, i.e., a growing number of schools introducing the humanities, a growth in the quality and scope of programs being offered, and a greater acceptance of the humanities as an integral part of the medical …

Scanning And Transmission Electron Microscopy Of The Oocyst Wall Of Isospora Lacazei, Clarence A. Speer, Alan A. Marchiondo, Bodo Mueller, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The oocyst wall of Isospora lacazei from sparrows was studied with scanning (SEM) and transmission (TEM) electron microscopy. In TEM, t he oocyst wall consisted of four distinct layers (Ll-4). The innermost layer, Ll, was moderately electron-lucent and 240-285 nm thick; L2 was electronJense and 210-240 nm thick; L3 was moderately electron-lucent and 15-150 nm thick; L4, the outer most layer, was discontinuous and consisted of electron-dense discoid bodies which measured 180-220 nm x 320-840 nm. The discoid bodies of L4 as seen by TEM appeared spheroid in shape when observed by SEM. One or two membranes were situated on …

Endoparasites, John E. Ubelaker, Robert D. Specian, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The leaf-nosed bats of the New World family Phyllostomatidae occur from the southwestern United States through tropical Central and South America. Members of this family are also found throughout the Antilles. The ecological associations of the species in this family seem to be rather broad; species are found in humid tropical to semiarid and arid subtropical environments. Diversity in feeding is readily apparent ranging from nectivores (Glossophaga), frugivores (Artibeus), sanguivores (Desmodus), to omnivores (Phyllostomus) (see review by Glass, 1970; Gardner, this volume).

To understand better the biology of phyllostomatid bats, it is …

Differentiation Of Fourth And Early Fifth Stages Of Parascaris Equorum (Goeze, 1782) Nematoda: Ascaridoidea, P. A. Pilitt, J. R. Lichtenfels, P. A. Madden

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The morphology of Parascaris equorum of horses was studied; light and scanning electron microscopy were used. Late fourth-stage larvae have narrow, rectangular lips bearing few, large. triangular, irregularly spaced denticles and the lips are not markedly set off from the body. The cutick of the late fourth stage is completely transversely striated, with incomplete longitudinal ridges creating a brickwork pattern; longitudinal alae are present. Total body lengths of fourth-stage larvae range from 10 to 32 mm. A single molting fourth-stage male was found to be 33 mm long. Early fifthstage nematodes have wide, trilobate lips with a deep transverse groove …

Paravitellotrema Overstreeti Sp. N. (Digenea: Hemiuridae) From The Colombian Freshwater Stingray Potamotrygon Magdalenae Dumeri, Daniel R. Brooks, Monte A. Mayers, Thomas B. Thorson

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Paravitellotrema overstreeti from the freshwater stingray Potamotrygon magdalenae in northern Colombia most closely resembles P. thorsoni by possessing a muscular sinus organ and sinus sac as well as exhibiting a saccate rather than elongate prostatic vesicle, It differs by possessing lobate rather than spherical vitellaria, a smaller sinus organ and sinus sac, elongate rather than diamond-shaped prostatic cells enclosed in a delicate membrane rather than free in the parenchyma, and a metraterm joining the hermaphroditic duct immediately anterior to the prostatic vesicle rather than at the base of the sinus organ,

Neopronocephalus Orientalis Sp. N. (Digenea: Pronocephalidae) And Spirhapalum Elongatum Rohde, Lee, And Lim, 1968 (Digenea: Spirorchiidae) From Cuora Amboinensis (Daudin) In Malaysia, Daniel R. Brooks, James R. Palmieri

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Neopronocephalus orientalis from Cuora amboinensis in Malaysia most closely resembles N. spinometraterminis from Kachuga tectum tentoria in India by possessing postovarian cecal tips and an average of more than 30 vitelline follicles, but differs by having equatorial rather than preequatorial testes which are mostly intercecal rather than extracecal and a slightly smaller cirrus sac, Neopronocephaius spinometraterminis purportedly has spines in the metraterm and a common genital pore, whereas N . orientalis exhibits nonstaining wrinkled epithelium lining the metraterm and separate genital pores. Spirhapalum elongatum was also collected from its type host near the type locality.

New Records For Amphibian And Reptile Trematodes, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

From 1975 to 1978, 200 amphibians and reptiles from localities in Nebraska, Arkansas, Oklahoma, Louisiana, Mississippi, Florida, North Carolina, and West Virginia were examined for trematode parasites. This report provides additional information concerning the geographical distribution of North American amphibian and reptile trematodes. Some results have already been published (Brooks and Fusco, 1978, J. Miss. Acad. Sci. 23:95-99). Worms, removed from their hosts, were examined alive when possible, and fixed with AFA after flattening with slight coverslip pressure. Specimens were stored in 7{)1lO ethanol, then stained with Mayer's hematoxylin and mounted in Histoclad or Canada balsam for study as whole …

Differentiation Of Fourth And Early Fifth Stages Of Parascaris Equorum (Goeze, 1782) Nematoda: Ascaridoidea, P. A. Pilitt, J. R. Lichtenfels, P. A. Madden

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The morphology of Parascaris equorum of horses was studied; light and scanning electron microscopy were used. Late fourth-stage larvae have narrow, rectangular lips bearing few, large . triangular, irregularly spaced denticles and the lips are not markedly set off from the body. The cuticle of the late fourth stage is completely transversely striated, with incomplete longitudinal ridges creating a brickwork pattern; longitudinal alae are present. Total body lengths of fourth-stage larvae range from 10 to 32 mm. A single molting fourth-stage male was found to be 33 mm long. Early fifthstage nematodes have wide, trilobate lips with a deep transverse …

Oligacanthorhynchus Lamasi (Freitas And Costa, 1964) Comb. N. From Domestic Cats Of Brazil, J. F.R. Amato, Brent B. Nickol, O. M. Froes

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Freitas and Costa (1964, Arq. Esc. Vet. Univ. Fed. Minas Gerais 16:231-234) described a new species, Echinopardalis lamasi, from two acanthocephalans (1 male, 1 female) of a cat, Felis domestica (=F. catus), captured in Belo Horizonte, Minas Gerais. These specimens have been lost (Freitas, 1975, personal communication). Until two of 127 domestic cats, F. catus, from Porto Alegre, Rio Grande do SuI, captured between October 1973 and July 1976, were found to harbor respectively two and nine acanthocephalans conspecific with those described by Freitas and Costa, no additional specimens of this species were available for study. These new specimens assist …

A Filamentous Bacterium On The Brine Shrimp And Its Control, Mobashir A. Solangi, Robin M. Overstreet, Ann L. Gannam

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A strain of a colorless, filamentous bacterium (tentatively identified as Leucothrix mucor) heavily infests the brine shrimp, Artemia salina. Its ultrastructure, unlike that of some other strains, does not reveal a distinct middle layer between its outer cell wall layer and cytoplasmic membrane, irregular blebs extending from the cell layers, or an external sheath. An entire infestation, represented as a mat of the bacterium with associated debris and microorganisms, sloughs from the shrimp when exposed to a variety of treatments. Primarily because most effective treatments are toxic to the shrimp, 100 ppm terramycin provides the treatment of choice.

Introduction: Harold W. Manter And Parasitology, Franklin Sogandares-Bernal

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Introductory remarks given by Franklin Sogandares-Bernal of Southern Methodist University at a symposium held at the University of Nebraska-Lincoln, Lincoln, Nebraska, U.S.A., October 5-7, 1977, on host-parasite interfaces. An overview of the career of American parasitologist Harold Winfred Manter, whose career was spent as a professor at the University of Nebraska-Lincoln, is presented.

Preface To Host-Parasite Interactions, Edited By Brent B. Nickol, Brent B. Nickol

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Preface to Host-Parasite Interactions, edited by Brent B. Nickol, proceedings of a symposium held at the University of Nebraska-Lincoln, Lincoln, Nebraska, U.S.A., October 5-7, 1977.

Dedication In Host-Parasite Interfaces, Edited By Brent B. Nickol, Brent B. Nickol

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Dedication in Host-Parasite Interfaces, edited by Brent B. Nickol, proceedings of a symposium held at the University of Nebraska-Lincoln, Lincoln, Nebraska, U.S.A., October 5-7, 1977.