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Poecilancistrium Caryophyllum And Other Trypanorhynch Cestode Plerocercoids From The Musculature Of Cynoscion Nebulosus And Other Sciaenid Fishes In The Gulf Of Mexico, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Examination of over 3,000 fish, all sciaenids, has shown that plerocercoids of Poecilancistrium caryophyllum infect Cynoscion nebulosus, Bairdiella chrysura, Sciaenops ocellata, C. arenarius, Micropogonias undulatus, and Pogonias cromis in Mississippi Sound. In addition to P. caryophyllum, a pseudophyllidean-like trypanorhynch also infected C. nebulosus and M. undulatus. Pseudogrillotia pleistacantha selectively infected large P. cromis, Pterobothrium heteracanthum infected M. undulatus, and Pterobothrium lintoni infected Menticirrhus americanus. Prevalence and intensity of infections of P. caryophyllum in C. nebulosus from Mississippi, roughly 40% of the fish each possessing an average of about two worms, …

Herpetomonas Megaseliae And Crithidia Harmosa: Growth On Blood-Agar Plates, Amy Doran Kepel, John J. Janovy Jr.

John J. Janovy, Jr. Publications

A technique for culturing two species of trypanosomatid flagellates on blood-agar plates is described. Herpetomonas megaseliae and Crithidia harmosa were grown as discrete clone colonies on plates prepared in sterile petri dishes with a medium derived from that of Tobie et al. (1950, J. Parasitol. 36: 48-54). Plates were inoculated with 0.01 ml flagellate suspension and the inoculum was spread evenly over the surface of the agar with a sterile glass spreader. Plates were incubated in a high humidity chamber at 25 C and colonies of both species, easily visible to the unaided eye, appeared within 1 week. There was …

Myxosoma Funduli Kudo 1918 (Protozoa: Myxosporida) In Fundulus Kansae: Summer Epizootiology, Stephen A. Knight, John J. Janovy Jr., William L. Current

John J. Janovy, Jr. Publications

The occurrence and distribution of the myxosporidan Myxosoma funduli on the gills of the plains killifish (Fundulus kansae) were investigated; Fundulus kansae is reported as a new host. Host samples from various sites on the South Platte River, Nebraska, were collected during the summer months of 1975 and 1976. The protozoan parasite population was shown to be overdispersed within the host population, and this distribution was similar to that described by the negative binomial equation. Demographic characteristics of the infected fish subpopulation were virtually identical to those of the whole fish population. Infection intensity was independent of gill …

Two New Zoogonid Digenea From Deep Sea Fishes In The Gulf Of Panama, Robin M. Overstreet, Mary Lou Hanson Pritchard

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Panopula cavernossa gen. et sp. n. from the intestine of the brotulid Enchelybrotula (?) paucidens,/i> differs from other members of the Steganodermatinae by having testes near the anterior of the acetabulum, a posttesticular ovary, and a postequatorial acetabulum. Two related species are transferred to the genus Brachyenteron as B. pycnorganum comb. n. and B. spinosum comb. n. Neosteganoderma gillissi sp. n. from the synaphobranchid eel Synaphobranchus bathybius differs from N. glandulosum and N. polymixiae, the most similar species, because conspicuous glandular cells envelop the esophagus and pharynx, others about the genital atrium occur in elongated sacs, epithelium is lacking …

Marine Aspidogastrids (Trematoda) From Fishes In The Northern Gulf Of Mexico, Sherman S. Hendrix, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Of the aspidogastrids Multicalyx cristata, Lobatostoma ringens, Cotylogaster basiri, and C. dinosoides sp. n., the last two had not been previously known from the Gulf of Mexico. The latter differs from other members of its genus by having relatively large equatorial marginal alveoli in comparison to those at the anterior and posterior ends of the holdfast. It also possesses extensive transverse musculature connecting opposed lateral alveoli. New host records are included for all four species, and we report preadults of what appear to be the first two species.

Nematicidal Injection: Targeted Control Of Plant-Parasitic Nematodes Of Trees And Vines, D. R. Viglierchio, Armand R. Maggenti, R. V. Schmitt, G. A. Paxman

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Pressurized injection of nematicidal solutions was effective for control of nematodes within trees and vines. Significant (P = 0.01) control of Pratylenchus vulnus on grape was attained with four nonfumigants (carbofuran, oxamyl, phenamiphos, and sulfocarb) and one fumigant nematicide (DBCP). Pratylenchus penetrans was controlled (P = 0.05 and 0.1) in apples and walnuts with sulfocarb and oxamyl. This species also was controlled in apples with carbofuran and phenamiphos. The advantages of pressure injection over traditional methods of nematicide applications are discussed.

Monoecocestus Thomasi Sp. N. (Cestoda: Anoplocephalidae) From The Northern Flying Squirrel, Glaucomys Sabrinus (Shaw), In Oregon, Robert L. Rausch, C. Maser

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Monoecocestus thomasi sp. n. (Cestoda: Anoplocephalidae), from the northern flying squirrel, Glaucomys sabrinus (Shaw), in Oregon, is described and distinguished from its congeners. Monoecocestus thomasi is the sixth species of Monoecocestus to be described from nearctic rodents, and the first from a member of the family Sciuridae. Our comparisons of taxonomic characters of the nominal species of Monoecocestus in North American rodents have shown that M. giganticus Buhler 1970 is a synonym of M. americanus (Stiles 1895), both from the porcupine, Erethizon dorsatum (Linnaeus). Insemination in Monoecocestus spp. is discussed, with the conclusion that it takes place only by way …

Research For Connecticut By The Storrs Agricultural Experiment Station

Storrs Agricultural Experiment Station

No abstract provided.

Demonstration Of Unique Mycelial Components In Candida Albicans, David Jon Mancuso

Biological Sciences Theses & Dissertations

Mycelial (M) and blastopore (B) cell homogenates of Candida albicans strain BJ85 were compared to detect and recover unique M components. Qualitative differences in the protein and lipid content of Mand B homogenates were suggested by discontinuous polyacrylamide gel electrophoresis (DPAGE). In addition, a quantitative and possibly qualitative shift in the protein content during germ tube formation was implied by DPAGE. Unique M components were detected using both DPAGE and immunoelectrophoretic (IE) techniques. One unique M component by DPAGE, four by IE, and two by combined DPAGE and IE techniques were suggested. Of these three methods, only DPAGE and the …

Effects Of Types 1 And 2 Herpes Simplex Viruses On Several Fish Tissue Culture Systems, Raymond Charles Harry

Dissertations and Theses

The purpose of this investigation was to observe changes induced by types 1 and 2 herpes simplex viruses (HSV 1 and HSV 2) when these viruses were cultivated in fish cells. The possibility of attempting to use human strains of herpesviruses in order to transform normal fish tissue culture preparations was considered. Three different fish cell cultures were tested: CHSE-214 cell lines were derived from embryonic chinook salmon (Oncorhynchus tshawytscha), and STE-137 cell lines were obtained from embryonic steelhead trout (Salmo gairdneri). Cells isolated from immature ovaries of rainbow trout (Salmo gairdneri) were propagated …

Subtle Teratogenic Effects Of Locoweed In Rats, Benjamin K. Nelson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Locoweed, a well-known teratogenic plant affecting livestock, is prevalent in mountain regions of the Western United States. Two common species (Astragalus lentiginosus and A. wootoni), administered to pregnant rats, induced behavioral deviations in their offspring. Treated mothers consumed less feed and gained less wieght during gestation than controls when gavaged locoweed at the rate of 1 gram whole plant per day on days 7 through 17 of gestation. A. lentiginosus reduced pup weight at birth (13% less than controls) and this weight reduction (as much as 29% less than controls) continued at least four weeks. A. lentiginosus also …

The Immunizing Potential Of Sporulated Oocysts Of Eimeria Nieschulzi Exposed To Heat And Co-60 Gamma-Radiation, George A. Conder, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Sporulated oocysts of Eimeria nieschulzi Dieben 1924, a rat coccidium, were exposed to radiation, heat, or both in an effort to attenuate the parasite. Moderate levels of each treatment or combination thereof attenuated the parasite, reduced pathogenesis (as judged by oocyst discharge during primary infection), and produced immunity to challenge when the oocysts were subsequently inoculated into rats. Thus, heat- and/or radiation-treated E. nieschulzi oocysts fed to rats could reduce pathogenesis during a primary infection and yet give good homologous protection.

Life History And Host Specificity Of Mediorhynchus Centurorum Nickol 1969 (Acanthocephala: Gigantorhynchidae), Brent B. Nickol

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Examination of 1905 arthropods from a swamp and surrounding pasture in southern Louisiana revealed larval specimens of Mediorhynchus centurorum in 8 of 228 woodroaches, Parcoblatta pensylvanica, but in no other species. The life cycle was confirmed by feeding eggs of M. centurorum to laboratory-reared woodroaches. Cystacanths later recovered from the woodroaches developed into mature worms when pipetted into esophaguses of red-bellied woodpeckers, Centurus carolinus; redheaded woodpeckers, Melanerpes erythrocephalus; yellow-shafted flickers, Colaptes auratus; and a hairy woodpecker, Dendrocopos villosus. Infection was achieved in all woodpeckers fed cystacanths at least 47 days old and the mean prepatent …

Aploparaksis Borealis Sp. N. (Cestoda: Hymenolepididae) From Passeriform And Charadriiform Birds In Chukotka And Alaska, S. K. Bondarenko, Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Aploparaksis borealis sp. n. (Cestoda: Hymenolepididae), from the long-tailed jaeger, Stercorarius longicaudus Vieillot, on the Seward Peninsula, Alaska, and from the red-throated pipit, Anthus cervinus (Pallas), and the Lapland longspur, Calcarius lapponicus (Linnaeus), in Chukotka, northeastern Siberia, is described. This cestode is distinguished by the structure of the cirrus and other morphologic characters from other species of Aploparaksis Clerc 1903 in which there are 10 rostellar hooks and the vitelline gland is aporal to the ovary. A. borealis is the first species of Aploparaksis to be reported from birds representing two orders (Passeriformes and Charadriiformes).

Immunization Of Rats Against Mesocestoides Corti (Cestoda) By Subcutaneous Vaccination Of Living Tetrathyridia And By Passive Transfer With Serum, Jerry Y. Niederkorn

Journal of the Arkansas Academy of Science

Laboratory rats were subcutaneously vaccinated with 100 live tetrathyridia of Mesocestoides corti (Cestoda) and subsequently intraperitoneally challenged with 50 tetrathyridia. Necropsy 30 days postinfection revealed that vaccinated rats harbored 97.4% fewer worms compared to control rats. In a second experiment, passive transfer of immunity was accomplished by immune serum from subcutaneously vaccinated rats. Rats receiving immune serum harbored 33.4% lighter worm burdens compared to normal serum recipients.

Vibriosis In Fish, G. L. Bullock

United States Fish and Wildlife Service: Publications

Fish vibriosis is a systemic disease of marine, estuarine, and some freshwater fishes, caused by bacteria of the genus Vibrio (Ross et al. 1968, Ghittino et al. 1972). The disease has been known for centuries; outbreaks along the Italian coast were recorded as early as the 1500's. Terms such as "red pest," "red boil," "red plague," or "saltwater furunculosis" have been applied to vibrio infections, but vibriosis is a more specific term and is now used by most fishery workers. With the rapid development of mariculture, vibriosis has become a major cause of fish loss--sometimes to the extent of being …

Progress In Mastitis Control : A Simple Control Programme That Works, G R. Olney, R. K. Mitchell

Journal of the Department of Agriculture, Western Australia, Series 4

Intensive Department of Agriculture work on the mastitis problem has resulted in development of a simple control programme that works in W.A. dairy herds.

Farmern receive regular advice on their herd mastitis levels, and advice is available on overcoming specific problems.

Spring Viremia Of Carp, Winfried Ahne, Ken Wolf

United States Fish and Wildlife Service: Publications

Dating back nearly SO years and possibly even to the Middle Ages, European pond culture of carp (Cyprinus carpio) and perhaps other cyprinid fishes has been plagued with a contagious disease of great importance. Variously known as infectious dropsy, infectious ascites, hemorrhagic septicemia, or rubella, the disease is probably the most serious cause of losses among these fishes; nearly sao reports have been published on the subject.

Studies On The Complement Receptor Of A Human Lymphoblastoid Cell Line, Gary John Hoffman

Student Theses and Dissertations

A human lymphoblastoid cell line, 8866, was used to investigate a surface marker, the C3d receptor. A method for cloning these cells at a relatively high efficiency was developed and C3d receptor bearing (CR+) and receptor free (CR-) subclones isolated. Receptor bearing cells were identified by their capacity to bind to complement coated sheep erythrocytes. Of the many clones isolated, only a few contained uniformly receptor positive or negative cells. Most expressed an intermediate character, not only in the percentage of CR+ cells, but also in the degree of resettability of the individual CR+ cells. Clones initially wholly CR+ remained …

Lumpy Wool May Increase By Dipping : Research Roundup, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

Lumpy wool, or mycotic dermatitis, is an infectious disease on the skin of the sheep caused by the bacterium Dermatophilus conglensis. The organism invades the wool and hair follicles, causing formation of skin lesions producing scabby material which binds the wool fibres into a hard mass.

Research shows that 'dipping' may increase the incidence of lumpy wool with some chemicals a greater risk than others.

Influence Of Season On Immunoglobulin Absorption And Status In Young Calves, Lynn Maureen Boyd

Electronic Theses and Dissertations

A high rate of calf mortality can represent a major economic loss to the dairy farmer. Despite increased knowledge in calf rearing, mortality rates of 10 to as high as 25% are not uncommon. The loss of heifer calves alone cost dairymen an estimated $81 million in 1975. This figure does not include the additional costs of extra labor, medication and veterinary expenses, and the loss of genetic potential. The most critical period for calf survival is the first 2 to 3 wk of life. During this time, the calf depends upon colostral immunoglobulins absorbed during the first 24 h …

Blood Parasites Of Squirrels From Coles And Lake Counties In Illinois, Nicholas I. Obiri

Masters Theses

Hepatozoon and microfilariae are described from the blood of fox and gray squirrels captured in Coles and Lake Counties in Illinois during 1976 and 1977. The hepatozoon species is identified as Hepatozoon griseisciuri on the basis of parasite morphology and hosts. A higher rate of infection (35.7% : 11.1%) was observed in gray squirrels than in fox squirrels and more adults were infected than juveniles. No microfilariae were found in gray squirrels but fox squirrels had a 57.1% infection rate with no difference based on age. The observed microfilariae are similar to that described by Davidson from Maryland gray squirrels …

Cotton Fireweed : Potential Poison : Research Roundup, J Dickson, R. Hill

Journal of the Department of Agriculture, Western Australia, Series 4

Although long known as a potential toxic species because of its content, and although it is widely distribuited in all Australian States, cotton fireweed has not generally been consdidered toxic because it is unpalatable and not usually eaten by stock.

In 1973 cattle deaths occured near Ravensthorpe which led to research into Senecio quadridentatus (cotton fireweed).

The plant must be seen as a potential toxic species in situations where the lack of other feed forces cattle to eat the normally unpalatable cotton fireweed.

Enucleation And Reconstruction Of Interferon-Producing Cells, D. C. Burke, G. Veomett

School of Biological Sciences: Faculty Publications

Enucleation of L cells leads to loss of the capacity to produce interferon, showing that the cell nucleus is essential for interferon formation. However, when the cells were enucleated while interferon formation was proceeding, the cytoplasts were capable of continuing to synthesize interferon by a process shown to be protein synthesis, showing that the interferon messenger RNA leaves the nucleus after synthesis. Reconstructed cells were obtained by Sendai virus fusion of karyoplasts and cytoplasts. Such reconstructed cells were capable of producing at least as much interferon (43 interferon units/10⁴ nucleated cells) as control cells (31 interferon units/10⁴ nucleated …

Absence Of Spermine In Filamentous Fungi, Kenneth W. Nickerson, Larry D. Dunkle, James L. Van Etten

Papers in Microbiology

Polyamines were examined in several yeasts and filamentous fungi. Whereas putrescine, spermidine, and spennine were present in the yeasts, spernine was not detected in any of the filamentous fungi.

Interrelationships Of Hypera Postica (Gyllenhal) (Coleoptera: Curculionidae) And Bathyplectes Curculionis (Thomson) (Hymenoptera: Ichneumonidae) In Illinois With Emphasis On Host-Parasite Densities, Distribution Of Parasites Within Available Hosts And Estimates Of Percent Parasitism, Robert John Barney

Masters Theses

In an attempt to better understand the host-parasite relationship of the alfalfa weevil, Hypera postica (Gyllenhal) (Coleoptera: Curculionidae) and Bathyplectes curculionis (Thomson) (Hymenoptera: Ichneumonidae), all life stages of the parasite were monitored throughout the spring. Sampling was conducted on a degree day basis from early March to late May in an alfalfa field in Mason County, Illinois.

Dissections of host alfalfa weevil larvae revealed 2nd and 3rd host instars were preferred larvae for oviposition. Peak parasite densities were found to precede peak total host larval densities, but coincided with peak densities of the preferred 2nd and 3rd instars. Larger host …

Susceptibility At High Inoculum Of Penicillin-Resistant Staphylococcus Aureus To Six Cephalosporins, Andres Suarez, Ramon Del Busto, Edward L. Quinn, Donald Pohlod

Henry Ford Hospital Medical Journal

Six cephalosporins (currently available for clinical use or undergoing clinical trials) were tested by tube dilution method against high inoculum (10⁷ colony forming units) of 37 strains of penicillin resistant Staphylococcus aureus. 3.1 μg/ml of cephalothin inhibited 100% of strains while higher concentrations of cefoxitin, cephapirin, cefamandole, cephalexin and cephradine were required. The percent of strains with minimal inhibitory concentration higher than 3.1 μg/ml ranged between 10.8% (cefoxitin) and 100% (cephradine). These findings support the observation that cephalothin is less susceptible to inactivation than the other cephalosporins. Although the exact clinical implication of these findings has not been established, …

Ultrastructure Of Interlamellar Henneguya Exilis In The Channel Catfish, William L. Current, John J. Janovy Jr.

John J. Janovy, Jr. Publications

Ultrastructural aspects of interlamellar Henneguya exilis infections in channel catfish are reported. The plasmodium wall of this form differs from that of other species in that it is composed of two outer unit membranes which give rise to a zone of numerous pinocytic canals. Single-membraned canals appeared to be a stable feature of the wall while double-membraned canals are interpreted as those actively carrying out pinocytosis. Evidence suggests that host cellular cytoplasm as well as interstitial material is taken in by plasmodia. Plasmodium wall integrity, aggregation of parasite ectoplasmic components, numbers of pinocytic canals, and number of mitochondria proximal to …

Ultrastructure Of The Sporocyst Wall During Excystation Of Isospora Endocallimici, Clarence A. Speer, Alan A. Marchiondo, Donald W. Duszynski, Sharon K. File

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Sporocysts of Isospora endocallimici, a parasite of marmosets, were exposed to minimal essentials medium (MEM) or a trypsin-bile salt solution (TBS) and then fixed and prepared for trans- mission electron microscopy. Excystation occurred in TBS but not MEM. The sporocyst wall has 2 layers, a thin outer layer (15 to 110 nm thick) and a thick inner layer (65 to 180 nm thick), which is composed of 4 separate curved plates. The outer layer consists of 1 to 3 membranes interspersed with lipid droplets. In the inner layer, a thin layer of material connects the peripheral margins of 2 …

Telorchis Gutturosi Sp. N. (Trematoda: Telorchiidae) From Graptemys Pseudogeographica Gray In Nebraska, With Reports Of Additional Species Of Trematodes From Nebraska Turtles, Daniel R. Brooks, Monte A. Mayes

Harold W. Manter Laboratory of Parasitology: Faculty Publications

One species of digenean is described and 8 others reported from turtles in Nebraska. Telorchis gutturosi, described from Graptemys pseudogeographica, resembles T. stossichi, T. pseudoaculeatus, and T. pleroticus in having an acetabulum significantly smaller than the oral sucker, but differs by possessing a pharynx which is as large as the acetabulum and having the ovary only 1/10 the body length from the acetabulum. Telorchis necturi is considered the senior synonym of T. cryptobranchi; Graptemys pseudogeographica is a new host. Hapalorhynchus stunkardi is reported for the first time since its description. Based on new morphological evidence, …