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Remote Sensing And Artificial Intelligence-Based Modeling And Prediction Of Harmful Algal Blooms In Lake Pontchartrain, Ian Smith May 2023

Remote Sensing And Artificial Intelligence-Based Modeling And Prediction Of Harmful Algal Blooms In Lake Pontchartrain, Ian Smith

LSU Master's Theses

Harmful Algal Blooms (HABs) and particularly toxic cyanobacterial harmful algal blooms (CyanoHABs) have become a growing threat to the environment, economy, communities, and human and animal health. This is particularly true for Lake Pontchartrain. Forecasting the occurrence of cyanobacterial HABs in Lake Pontchartrain is a process that currently does not exist, as nowcasting by the NCCOS Algal Bloom Monitoring System or the similar system implemented by the U.S. EPA is currently the only method to monitor HAB production in the Lake. This thesis made this process possible by identifying antecedent environmental conditions controlling CyanoHABs, describing the conditions using NASA satellite …


A Study On Image Processing Techniques And Deep Learning Techniques For Insect Identification, Vinita Abhishek Gupta, M.V. Padmavati, Ravi R. Saxena, Pawan Kumar Patnaik, Raunak Kumar Tamrakar May 2023

A Study On Image Processing Techniques And Deep Learning Techniques For Insect Identification, Vinita Abhishek Gupta, M.V. Padmavati, Ravi R. Saxena, Pawan Kumar Patnaik, Raunak Kumar Tamrakar

Karbala International Journal of Modern Science

Automatic identification of insects and diseases has attracted researchers for the last few years. Researchers have suggested several algorithms to get around the problems of manually identifying insects and pests. Image processing techniques and deep convolution neural networks can overcome the challenges of manual insect identification and classification. This work focused on optimizing and assessing deep convolutional neural networks for insect identification. AlexNet, MobileNetv2, ResNet-50, ResNet-101, GoogleNet, InceptionV3, SqueezeNet, ShuffleNet, DenseNet201, VGG-16 and VGG-19 are the architectures evaluated on three different datasets. In our experiments, DenseNet 201 performed well with the highest test accuracy. Regarding training time, AlexNet performed well, …


Assessing The Release Of Microplastics And Nanoplastics From Plastic Containers And Reusable Food Pouches: Implications For Human Health, Kazi Albab Hussain, Svetlana Romanova, Ilhami Okur, Dong Zhang, Jesse Kuebler, Xi Huang, Bing Wang, Lucia Fernandez-Ballester, Yongfeng Lu, Mathias Schubert, Yusong Li May 2023

Assessing The Release Of Microplastics And Nanoplastics From Plastic Containers And Reusable Food Pouches: Implications For Human Health, Kazi Albab Hussain, Svetlana Romanova, Ilhami Okur, Dong Zhang, Jesse Kuebler, Xi Huang, Bing Wang, Lucia Fernandez-Ballester, Yongfeng Lu, Mathias Schubert, Yusong Li

Department of Civil and Environmental Engineering: Faculty Publications

This study investigated the release of microplastics and nanoplastics from plastic containers and reusable food pouches under different usage scenarios, using DI water and 3% acetic acid as food simulants for aqueous foods and acidic foods. The results indicated that microwave heating caused the highest release of microplastics and nanoplastics into food compared to other usage scenarios, such as refrigeration or room-temperature storage. It was found that some containers could release as many as 4.22 million microplastic and 2.11 billion nanoplastic particles from only one square centimeter of plastic area within 3 min of microwave heating. Refrigeration and room-temperature storage …


Biologically Inspired Multi-Robot System Based On Wolf Hunting Behavior, Zachary Hinnen, Chance Hamilton, Alfredo Weitzenfeld May 2023

Biologically Inspired Multi-Robot System Based On Wolf Hunting Behavior, Zachary Hinnen, Chance Hamilton, Alfredo Weitzenfeld

Florida Conference on Recent Advances in Robotics

Studies involving the group predator behavior of wolves have inspired multiple robotic architectures to mimic these biological behaviors in their designs and research. In this work, we aim to use robotic systems to mimic wolf packs' single and group behavior. This work aims to extend the original research by Weitzenfeld et al [7] and evaluate under a new multi-robot robot system architecture. The multiple robot architecture includes a 'Prey' pursued by a wolf pack consisting of an 'Alpha' and 'Beta' robotic group. The Alpha Wolf' will be the group leader, searching and tracking the 'Prey.' At the same time, the …


Extracellular-Vesicle-Based Therapeutics In Neuro-Ophthalmic Disorders, Hamed Massoumi, Sohil Amin, Mohammad Soleimani, Bita Momenaei, Mohammad Javad Ashraf, Victor H Guaiquil, Peiman Hematti, Mark I Rosenblatt, Ali R Djalilian, Elmira Jalilian May 2023

Extracellular-Vesicle-Based Therapeutics In Neuro-Ophthalmic Disorders, Hamed Massoumi, Sohil Amin, Mohammad Soleimani, Bita Momenaei, Mohammad Javad Ashraf, Victor H Guaiquil, Peiman Hematti, Mark I Rosenblatt, Ali R Djalilian, Elmira Jalilian

Wills Eye Hospital Papers

Extracellular vesicles (EVs) have been recognized as promising candidates for developing novel therapeutics for a wide range of pathologies, including ocular disorders, due to their ability to deliver a diverse array of bioactive molecules, including proteins, lipids, and nucleic acids, to recipient cells. Recent studies have shown that EVs derived from various cell types, including mesenchymal stromal cells (MSCs), retinal pigment epithelium cells, and endothelial cells, have therapeutic potential in ocular disorders, such as corneal injury and diabetic retinopathy. EVs exert their effects through various mechanisms, including promoting cell survival, reducing inflammation, and inducing tissue regeneration. Furthermore, EVs have shown …


Noninvasively Monitoring Of Cerebral Blood Flow In Piglet Models Of Graded Hemorrhage And Hypoxic Ischemic Brain Injury Using Diffuse Correlation Spectroscopy And Near-Infrared Spectroscopy, Randolph Sinahon, Danielle Shoshany, Shadi Malaeb, Mert Deniz Polat, Meltem Izzetoglu, Kurtulus Izzetoglu May 2023

Noninvasively Monitoring Of Cerebral Blood Flow In Piglet Models Of Graded Hemorrhage And Hypoxic Ischemic Brain Injury Using Diffuse Correlation Spectroscopy And Near-Infrared Spectroscopy, Randolph Sinahon, Danielle Shoshany, Shadi Malaeb, Mert Deniz Polat, Meltem Izzetoglu, Kurtulus Izzetoglu

St. Chris Research Day

No abstract provided.


Cerebral Blood Flow Measured By Diffuse Correlation Spectroscopy For Monitoring Depth Of Anesthesia In Piglets, Mert Deniz Polat, Kurtulus Izzetoglu, Randolph Sinahon, Meltem Izzetoglu, Shadi Malaeb May 2023

Cerebral Blood Flow Measured By Diffuse Correlation Spectroscopy For Monitoring Depth Of Anesthesia In Piglets, Mert Deniz Polat, Kurtulus Izzetoglu, Randolph Sinahon, Meltem Izzetoglu, Shadi Malaeb

St. Chris Research Day

No abstract provided.


Tech Time May 2023

Tech Time

DePaul Magazine

DePaul is embracing tech more than ever, incorporating innovative devices and approaches into education in all corners of the university. Here are seven ways DePaul provides hands-on experiences with cutting-edge tools that position students and faculty in the forefront of their industries and disciplines.


Enhanced Throughput Of Aligned Polyacrylonitrile Nanofiber Manufacturing Via High-Speed Centrifugal Spinning, Jason Glen Ippolito May 2023

Enhanced Throughput Of Aligned Polyacrylonitrile Nanofiber Manufacturing Via High-Speed Centrifugal Spinning, Jason Glen Ippolito

Theses and Dissertations

Centrifugal spinning is noteworthy due to its high fiber throughput rate compared to other nanofiber manufacturing platforms. It is capable of producing fibers in the nanoscale diameter range from a multitude of polymers, such as polyacrylonitrile (PAN). With a traditional centrifugal spinner, fiber can be rapidly spun and collected on static posts. However, the use of static posts inevitably forms a dense ring of fiber after a short period of time, and this fiber build up can halt the spinning process. In this work, the factors that influence the throughput and scalability of highly aligned centrifugally spun PAN fibers are …


A Neutrosophic Framework For Assessment Of Distributed Circular Water To Give Neighborhoods Analysis To Prepare For Unexpected Stressor Events, Mohamed Abouhawwash, Mohammed Jameel, Sameh S. Askar May 2023

A Neutrosophic Framework For Assessment Of Distributed Circular Water To Give Neighborhoods Analysis To Prepare For Unexpected Stressor Events, Mohamed Abouhawwash, Mohammed Jameel, Sameh S. Askar

Neutrosophic Systems with Applications

The global water issue is caused by a number of factors, including extreme weather, population increase, and industrial activity. One of the reasons we're running out of water as a planet is because of the outdated take-make-use-throw-away linear paradigm of handling water. It has been suggested that the circular economy may help alleviate water shortages by inspiring a fundamental change in municipal water infrastructure. Reduced consumption, recovered natural resources, and minimal waste are the three pillars of a circular water supply. A dispersed water supply is more adaptable and robust than a centralized one because it gives communities more time …


Modern Practices For Responsive Web Design And Web Accessibility, Keyaun Washington May 2023

Modern Practices For Responsive Web Design And Web Accessibility, Keyaun Washington

Honors Program: Senior Projects (Public)

Responsive web design and web accessibility play crucial roles in ensuring an optimal user experience on the web. By designing websites with responsiveness and accessibility in mind, more opportunities are opened up for a wider audience to access and interact with our content. Through modern practices, responsive web design allows websites to reach several different devices ranging from compact smartwatches to expansive television screens. Designing for accessibility provides accommodations for individuals with impairments while also providing benefits for individuals without impairments. However, designing for responsiveness and accessibility can present challenges; a poor attempt at providing accessibility features can worsen a …


Columbus, Nebraska: Southern Housing Development Design Proposal, Ryan Weyers, Caiden Dynek, Alex Papa, Brexton Robertson, Kaden Perala May 2023

Columbus, Nebraska: Southern Housing Development Design Proposal, Ryan Weyers, Caiden Dynek, Alex Papa, Brexton Robertson, Kaden Perala

Honors Program: Senior Projects (Public)

The following paper proposes the design and creation of a new housing development in the city of Columbus, Nebraska in a lot to the south of the city and directly north of the Columbus wastewater treatment plant. The goal of this proposal is to provide readers with confidence that our company is the most qualified to complete the project and will create the safest, most comfortable, most efficient, and most cost-effective final design. This proposal seeks to offer a solution to the lack of affordable housing for the growing workforce in Columbus. In order to arrive at this solution, each …


Piezoelectric And Conductive Polymer Based Flexible Devices Enabling Cardiovascular Health Sensing And Energy Harvesting, Andrew Closson May 2023

Piezoelectric And Conductive Polymer Based Flexible Devices Enabling Cardiovascular Health Sensing And Energy Harvesting, Andrew Closson

Dartmouth College Ph.D Dissertations

Piezoelectric materials show great promise for low-power wearable and implantable sensing, but their rigidity makes it challenging to integrate them with biological tissue. To address this, researchers have started exploring polymer-based functional materials that offer flexibility and are suitable for interfacing with the human body. However, these materials are still in their early stages, and a framework is necessary to illustrate how these materials, in conjunction with novel fabrication techniques and device designs, can enable the development of multi-functional sensing and energy harvesting devices.

This thesis utilizes highly scalable fabrication methods for functional polymers to build and test a flexible …


The Efficacy Of Conservation Practices In Reducing Floods And Improving Water Quality, Shivendra Srivastava, Andrea D. Basche, Elbert Traylor, Tirthankar Roy May 2023

The Efficacy Of Conservation Practices In Reducing Floods And Improving Water Quality, Shivendra Srivastava, Andrea D. Basche, Elbert Traylor, Tirthankar Roy

Department of Civil and Environmental Engineering: Faculty Publications

Conservation practices such as crop rotation, filter strips, and constructed wetlands are nature-based approaches intended to safeguard natural resources in agricultural landscapes. In this study, we reviewed the literature on how conservation practices, both at watershed and field scales, have been proven to subdue flood peaks, surface runoff, soil erosion, sediment transport, and nutrient loss. We classified different conservation practices based on the mode of their application (i.e., in-field, edge-of-field, and structural practices) and described what prior research efforts have concluded about the efficacy of different practices. At the field scale, practices such as reduced or no-till farming, grassed waterways, …


Dual Color Optogenetic Control For Analyzing Cardiac Function In Drosophila, Jiantao Zhu May 2023

Dual Color Optogenetic Control For Analyzing Cardiac Function In Drosophila, Jiantao Zhu

McKelvey School of Engineering Graduate Student Theses & Dissertations

Prolonged consumption of carbohydrate-rich diets and immobile lifestyles frequently cause metabolic disorders and obesity and, as a result, may lead to progressive heart dysfunction among broad social groups of the population. Drosophila melanogaster serves as an essential model organism in cardiovascular disease research due to conserved physiological and genomic traits shared with humans, its genetic and molecular toolbox versatility, and cost-effective maintenance. Here, we combine optogenetics and optical coherence tomography to study cardiovascular function in D. melanogaster. A new optogenetic pacing system has been developed, employing a transgenic line carrying two opsins: ChR2 and NpHR2.0. A custom-built hardware setup …


3-Dimensional Visualization Of Cardiac Plaque Mapping Data, Phan Ly Vy Nguyen May 2023

3-Dimensional Visualization Of Cardiac Plaque Mapping Data, Phan Ly Vy Nguyen

McKelvey School of Engineering Graduate Student Theses & Dissertations

Atrial Fibrillation (AF) of one of the most prevalent cardiac arrythmia in humans, and also the most studied arrythmias due to its high association with cardiovascular morbidity and mortality. Diagnosis of AF, which is highly dependent on the observation of the irregular signal in the atria, is often challenging since AF is often asymptomatic at the onset. There has been a lot of effort in exploring different cardiac mapping techniques to understand the dynamics of AF for better intervention. This study aims at developing a MATLAB interface that assists the development of a cardiac plaque mapping data acquisition system in …


Preparing Non-Human Primates To Study Hand-Eye Coordination In Frontal Eye Fields (Fef) During Delayed Movement Task, Juliusz Cydzik May 2023

Preparing Non-Human Primates To Study Hand-Eye Coordination In Frontal Eye Fields (Fef) During Delayed Movement Task, Juliusz Cydzik

McKelvey School of Engineering Graduate Student Theses & Dissertations

ABSTRACT OF THE THESIS Preparing Non-Human Primates to Study Hand-Eye Coordination in Frontal Eye Fields (FEF) During Delayed Movement Task by Juliusz Cydzik Master of Science in Biomedical Engineering Washington University in St. Louis, 2023 Professor Lawrence Snyder, Chair Hand-eye coordination enables humans and non-human primates to use their hands and eyes to perform various tasks. We are interested in coordination at the systems level, where saccades and reaches are encoded. The parietal reach region (PRR), situated at the posterior end of the intraparietal sulcus (IPS) and overlapping portions of the medial intraparietal area (MIP) and V6a, is commonly attributed …


The Biological Activity Of Dual-Responsive Glycopolymers For Intracellular Codelivery Of Antigen And Lipophilic Adjuvant, Micaela Shields May 2023

The Biological Activity Of Dual-Responsive Glycopolymers For Intracellular Codelivery Of Antigen And Lipophilic Adjuvant, Micaela Shields

Honors Theses

Subunit vaccines present themselves as an attractive alternative to traditional approaches to vaccines, which utilizes whole organisms to trigger an immune response. While these traditional approaches have proven themselves reliable within the history of vaccinations, they generally deliver subpar robust cellular-mediated immunity and pose many safety risks. Composed of proteins and /or peptides, subunit vaccines are safer and more precise alternatives to traditional approaches. However, the pharmacokinetic properties of the macromolecules that make up these vaccines hinder the subunit vaccine’s level of immunogenicity [d1] Examples of this hindrance include but are not limited to aggregation at the injection site and …


Continuous Bioprocessing: Technology For Next-Generation Biopharmaceutical Manufacturing Development Of A Python-Coded Bench-Scale Raman-Based Continuous Bioprocess Platform, Christine Urrea May 2023

Continuous Bioprocessing: Technology For Next-Generation Biopharmaceutical Manufacturing Development Of A Python-Coded Bench-Scale Raman-Based Continuous Bioprocess Platform, Christine Urrea

KGI Theses and Dissertations

Current industrial practices for producing biopharmaceuticals include fed-batch production with batch isolation and purification. Nonproductive hold-up steps and manual offline measurements are common in batch processing which increases processing time and contributes to a high cost of production. Current market trends and cost pressures in the biopharmaceutical industry are creating a push to innovate bioprocessing platforms. Continuous bioprocessing has been considered a solution to the current limitations of batch production of biopharmaceuticals. Continuous bioprocessing involves intensifying individual processing steps by eliminating hold-up steps through a continuous operation to increase productivity, which results in advantages such as lower capital and production …


Antimicrobial Activity And Mechanism Of Amyloid Proteins And Synthetic Conjugated Polyelectrolytes, Fahimeh Maghsoodi May 2023

Antimicrobial Activity And Mechanism Of Amyloid Proteins And Synthetic Conjugated Polyelectrolytes, Fahimeh Maghsoodi

Nanoscience and Microsystems ETDs

Although the exact cause of Alzheimer’s disease (AD) is still unknown, it is widely considered that the accumulation of amyloid plaques composed of the amyloid-β (Aβ) peptide in the brain is linked to neurodegeneration. Co-localization of viral DNA with Aβ plaques, the association of brain infection and AD, and research indicating the protective effect of Aβ against bacteria and fungi in mice and human cells have led to the hypothesis that Aβ expression and deposition may be central to its function as an antimicrobial peptide (AMP). In my thesis research, we seek to elucidate how Aβ functions as an AMP …


3d Printing Of Earthen Materials: Toward The Carbon-Zero Construction, Shiva Bhusal May 2023

3d Printing Of Earthen Materials: Toward The Carbon-Zero Construction, Shiva Bhusal

Civil Engineering ETDs

In recent years, digital manufacturing techniques in the architecture and construction industry have rapidly gained attention, especially with 3D printing, also called additive manufacturing. The use of cement has been common in modern construction and is now being explored for 3D printing. However, due to cement's contribution to approximately 7 percent of global carbon emissions and its high cost, research on sustainable and greener materials has gained attention. Previous studies on alternative construction have examined the mechanical and rheological properties of soil mixes, but not much attention has been given to printability evaluation, shrinkage, and cracking, which can affect the …


Design Of University Small-Scale Dairy Processing Facility, Philip J. Hildebrand, Joshua Arejola, Alicia Andersen, Terry Howell Jr, Tami M. Brown-Brandl May 2023

Design Of University Small-Scale Dairy Processing Facility, Philip J. Hildebrand, Joshua Arejola, Alicia Andersen, Terry Howell Jr, Tami M. Brown-Brandl

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This project was assigned to research the feasibility and value of implementing a dairy processing facility on the campus of the University of Nebraska-Lincoln. The facility would process milk produced from cows under the same roof and will serve as an educational experience for Nebraska dairy farmers, UNL students, and K-12 students in the Lincoln-Lancaster County area. If the project is successful and replicated across the state, this facility could have a significant impact on the reduction of milk transportation costs in the Nebraska dairy industry.

The project began with researching milk processing methods and steps from production to consumption. …


An Investigation Into The Common Problems Of Lagoon Wastewater Treatment In Kentucky, Kaylee A. Jones May 2023

An Investigation Into The Common Problems Of Lagoon Wastewater Treatment In Kentucky, Kaylee A. Jones

Honors Theses

Lagoon wastewater treatment systems are commonly used in areas that do not have the available resources for typical mechanical wastewater treatment. Lagoon systems consist of multiple preliminary lagoons, but mainly rely on long-term facultative lagoons for most analyte removal. In this study, one particular lagoon wastewater treatment system (named Municipality A Wastewater Treatment Plant) is investigated due to noncompliance with governmental standards provided by the National Pollutant Discharge Elimination System (NPDES). Samples were collected by researchers at 4 different instances inside of the treatment systems. These samples were sent to an analytical laboratory for testing. The readings from these samples …


Evaluation Of Conservation Practices Effect On Water Quality Using The Swat Model, Vivek Venishetty May 2023

Evaluation Of Conservation Practices Effect On Water Quality Using The Swat Model, Vivek Venishetty

Theses and Dissertations

The deterioration of water quality due to human-driven alternations has an adverse effect on the environment. More than 50% of surveyed surface water bodies in the United States (US) are classified as impaired waters as per the Clean Water Act. The pollutants affecting the water quality in the US are classified as point and non-point sources. Pollutant mitigation strategies such as the selective implementation of best management practices (BMPs) based on the severity of the pollution could improve water quality by reducing the amounts of pollutants. Quantifying the efficiency of a specific management practice can be difficult for large watersheds. …


Wildfire-Altered Geotechnical Soil Properties And Their Impact On Subsequent Landslides, James M. Kellogg May 2023

Wildfire-Altered Geotechnical Soil Properties And Their Impact On Subsequent Landslides, James M. Kellogg

Theses and Dissertations

Wildfires result in altered landscape as well as altered soil properties. Wildfires result in conditions that are more susceptible to geotechnical hazards introducing a potential for cascading events in the surrounding area. Shallow landslides are common geohazards that result in a post-wildfire landscape. With an ever-increasing trend in wildfires, the need to better understand the impact wildfire-altered soil properties play on shallow slope instability is critical to evaluating risks to existing or planned development, mitigation of potentially hazardous conditions, prioritization of post-wildfire planning, and post-wildfire response preparation. The primary objective of this study is to provide a comprehensive overview of …


Integrated Neutrosophic Best-Worst Method For Comprehensive Analysis And Ranking Of Flood Risks: A Case Study Approach From Aswan, Egypt, Safa Al-Saeed, Nabil M. Abdelaziz May 2023

Integrated Neutrosophic Best-Worst Method For Comprehensive Analysis And Ranking Of Flood Risks: A Case Study Approach From Aswan, Egypt, Safa Al-Saeed, Nabil M. Abdelaziz

Neutrosophic Systems with Applications

This paper presents a comprehensive framework for the analysis and ranking of flood risks with respect to regional variations and data uncertainties under a Neutrosophic environment. The research introduces a novel approach to flood risk mitigation and management, bringing together the scientifically robust Best-Worst Method (BWM) and single-valued Neutrosophic set for the first time. The unique application of a single-valued Neutrosophic set allows us to better illustrate and manage uncertainty, imprecision, and vagueness in data. Additionally, we employ BWM, a multi-factor decision-making method, for discerning and ranking the most influential flood risk factors. Together, the integrated methodologies provide a balanced, …


Introduction To Data-Driven Systems For Plastics And Composites Manufacturing, Saeed Farahani, Srikanth Pilla, Yun Zhang, Fausto Tucci May 2023

Introduction To Data-Driven Systems For Plastics And Composites Manufacturing, Saeed Farahani, Srikanth Pilla, Yun Zhang, Fausto Tucci

Mechanical Engineering Faculty Publications

Applications of high-performance plastics and composites have widely been expanded to various industries due to their superior properties, such as high strength-to-weight ratio, chemical resistance, and thermal/electrical insulation. However, the numerous possible combinations of polymers and reinforcements/fillers, the variability of these materials, and their complex manufacturing processes pose challenges in terms of efficiently developing new plastics and composites, accurately modeling their properties, and effectively monitoring and controlling their manufacturing processes. Integrating data-driven techniques, such as machine learning, artificial intelligence, and big data analytics, is a promising pathway to overcome these challenges as it is demonstrated by the state-of-the-art research works …


Soil Respiration Measurements Reveal High Retention Of Organic Carbon From Corn Residue Derived High-Lignin Fermentation Byproduct Enabling Sustainable Lignocellulosic Biofuel Production, Michelle Sun Wang May 2023

Soil Respiration Measurements Reveal High Retention Of Organic Carbon From Corn Residue Derived High-Lignin Fermentation Byproduct Enabling Sustainable Lignocellulosic Biofuel Production, Michelle Sun Wang

Dartmouth College Master’s Theses

While 2G biofuel production can utilize non-edible, lignocellulosic feedstocks such as agricultural residues to produce liquid fuel, harvesting crop residues is unsustainable without careful management of the soil underneath. By harvesting a fraction of the crop residues left in the field after harvest, soil health can diminish and critically, the soil organic carbon (SOC) stored in agricultural fields can decrease. Currently, in the most popular 2G process models published, the issue of soil degradation remains unresolved with residue harvest strategies receiving considerable attention in the literature and other SOC management strategies receiving far less. Specifically, the strategy of returning the …


Du Undergraduate Showcase: Research, Scholarship, And Creative Works, Caitlyn Aldersea, Justin Bravo, Sam Allen, Anna Block, Connor Block, Emma Buechler, Maria De Los Angeles Bustillos, Arianna Carlson, William Christensen, Olivia Kachulis, Noah Craver, Kate Dillon, Muskan Fatima, Angel Fernandes, Emma Finch, Colleen Cassidy, Amy Fishman, Andrea Francis, Stacia Fritz, Simran Gill, Emma Gries, Rylie Hansen, Shannon Powers, Jacqueline Martinez, Zachary Harker, Ashley Hasty, Mykaela Tanino-Springsteen, Kathleen Hopps, Adelaide Kerenick, Colin Kleckner, Ci Koehring, Elijah Kruger, Braden Krumholz, Maddie Leake, Lyneé Alves, Seraphina Loukas, Yatzari Lozano Vazquez, Haley Maki, Emily Martinez, Sierra Mckinney, Audrey Mitchell, Kipling Newman, Audrey Ng, Megan Lucyshyn, Andrew Nguyen, Stevie Ostman, Casandra Pearson, Alexandra Penney, Julia Gielczynski, Tyler Ball, Anna Rini, Christina Rorres, Simon Ruland, Helayna Schafer, Emma Sellers, Sarah Schuller, Claire Shaver, Kevin Summers, Isabella Shaw, Madison Sinar, Claudia Pena, Apshara Siwakoti, Carter Sorensen, Madi Sousa, Anna Sparling, Alexandra Revier, Brandon Thierry, Dylan Tyree, Maggie Williams, Lauren Wols May 2023

Du Undergraduate Showcase: Research, Scholarship, And Creative Works, Caitlyn Aldersea, Justin Bravo, Sam Allen, Anna Block, Connor Block, Emma Buechler, Maria De Los Angeles Bustillos, Arianna Carlson, William Christensen, Olivia Kachulis, Noah Craver, Kate Dillon, Muskan Fatima, Angel Fernandes, Emma Finch, Colleen Cassidy, Amy Fishman, Andrea Francis, Stacia Fritz, Simran Gill, Emma Gries, Rylie Hansen, Shannon Powers, Jacqueline Martinez, Zachary Harker, Ashley Hasty, Mykaela Tanino-Springsteen, Kathleen Hopps, Adelaide Kerenick, Colin Kleckner, Ci Koehring, Elijah Kruger, Braden Krumholz, Maddie Leake, Lyneé Alves, Seraphina Loukas, Yatzari Lozano Vazquez, Haley Maki, Emily Martinez, Sierra Mckinney, Audrey Mitchell, Kipling Newman, Audrey Ng, Megan Lucyshyn, Andrew Nguyen, Stevie Ostman, Casandra Pearson, Alexandra Penney, Julia Gielczynski, Tyler Ball, Anna Rini, Christina Rorres, Simon Ruland, Helayna Schafer, Emma Sellers, Sarah Schuller, Claire Shaver, Kevin Summers, Isabella Shaw, Madison Sinar, Claudia Pena, Apshara Siwakoti, Carter Sorensen, Madi Sousa, Anna Sparling, Alexandra Revier, Brandon Thierry, Dylan Tyree, Maggie Williams, Lauren Wols

DU Undergraduate Research Journal Archive

DU Undergraduate Showcase: Research, Scholarship, and Creative Works


Consolidated Chamber Design And Protocol For Olfactory Conditioning Assay With Drosophila Melanogaster, Sasha Bronovitskiy, Andres Castillo, Michael Yan, Fang Ju Lin May 2023

Consolidated Chamber Design And Protocol For Olfactory Conditioning Assay With Drosophila Melanogaster, Sasha Bronovitskiy, Andres Castillo, Michael Yan, Fang Ju Lin

Journal of the South Carolina Academy of Science

The olfactory conditioning assay is widely used in Alzheimer’s disease research to quantify learning and memory in Drosophila melanogaster. The assay tests ability to recall an aversive conditioned stimulus of scent paired with electrical shock when presented a choice between shock-associated and unrelated scents. The T-maze, a commonly used apparatus for olfactory conditioning assays, employs an elevator mechanism to transfer live flies from the shock-delivering training chamber to the scent selection point. This elevator mechanism is known to cause fly casualty. T-mazes are not commercially available and often difficult to reproduce. Other existing variations of olfactory conditioning apparatuses use …