1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine (Mptp)-Treated Adult Zebrafish As A Model For Parkinson’S Disease, Emmeline Bagwell

All Theses

The use of adult zebrafish, Danio rerio, as a model for human neurodegenerative diseases has proven valuable in pharmaceutical development and genetic disease research due to their high genetic homology to humans, cost-effective husbandry, and quick life cycle breeding times. Despite their promise, most zebrafish research has focused on embryonic stage organisms, primarily due to the ease of direct handling protocols and rapid progression through developmental stages. Recognizing the limitations of early-stage organisms in modeling adult diseases, recent efforts have shifted the applicability of zebrafish models, particularly for Parkinson's Disease, due to the nature of the disease impacting patients 55 …

Material World, Nicole Weldy

All Theses

My thesis, Material World, delves into the use of the crochet unit as a construction technique for building forms. Through this, I aim to organize different materials in a way that responds to the challenges posed by the physical world. My artistic process is centered around honoring the inherent qualities of thread and uses these qualities to create form, lightness, and linearity. At the same time, I remain receptive to transformative processes such as combining three-dimensional (3D) printed lines and lace stiffener to push the boundaries of what thread can do. By combining manual craftsmanship with technology materialized as …

Aicropcam: Deploying Classification, Segmentation, Detection, And Counting Deep-Learning Models For Crop Monitoring On The Edge, Nipuna Chamara, Geng (Frank) Bai, Yufeng Ge

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Precision Agriculture (PA) promises to meet the future demands for food, feed, fiber, and fuel while keeping their production sustainable and environmentally friendly. PA relies heavily on sensing technologies to inform site-specific decision supports for planting, irrigation, fertilization, spraying, and harvesting. Traditional point-based sensors enjoy small data sizes but are limited in their capacity to measure plant and canopy parameters. On the other hand, imaging sensors can be powerful in measuring a wide range of these parameters, especially when coupled with Artificial Intelligence. The challenge, however, is the lack of computing, electric power, and connectivity infrastructure in agricultural fields, preventing …

Determining The Presence And Size Of Shoulder Lesions In Sows Using Computer Vision, Shubham Bery, Tami M. Brown-Brandl, Bradley T. Jones, Gary A. Rohrer, Sudhendu Raj Sharma

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Shoulder sores predominantly arise in breeding sows and often result in untimely culling. Reported prevalence rates vary significantly, spanning between 5% and 50% depending upon the type of crate flooring inside a farm, the animal’s body condition, or an existing injury that causes lameness. These lesions represent not only a welfare concern but also have an economic impact due to the labor needed for treatment and medication. The objective of this study was to evaluate the use of computer vision techniques in detecting and determining the size of shoulder lesions. A Microsoft Kinect V2 camera captured the top-down depth and …

A Tessellation Of Engineers, Grace Jenkins Welsh

University Honors Theses

A capstone project is the culmination of what a student has learned while pursuing an undergraduate degree. A project of this magnitude requires a lot of time and dedication from every member of the team. This essay looks back at the experience of an electrical engineering student participating in the design of an auxiliary power and protection of a planned substation. The author describes the team's work, how they performed as a team, and their involvement with the professional sponsor and advisor. The author examines the experience with the goal of informing and encouraging prospective engineering students who may be …

Assessing The Thermodynamic Potential Of Deep Eutectic Solvents In The Absorption Of Greenhouse Gases Through Computational Methods, Thomas Michael Quaid

Theses and Dissertations

Greenhouse gas (GHG) capture is a fundamental technology in the fight against climate change. Some species of devastating GHGs like fluorinated compounds are released directly to the air from industrial processes. Some GHGs are co-produced in sustainably derived biofuels which require absorptive upgrading. The development of more efficient, cost effective, and environmentally friendly methods of capturing GHG’s from pollutant sources and industrial streams is of utmost importance in the fight against climate change. Deep eutectic solvents have entered the separations stage as potential disruptors to conventional solvent choices for a variety of applications. Deep eutectic solvents (DES) are compounds of …

Project Thetis: A Low-Cost, Low-Profile Inertial Data Logger, Braidan Duffy

Theses and Dissertations

This thesis details the design, testing, calibration, and verification of a nine degree of freedom inertial measurement data logger for use with floating bodies. The instrument was conceived to address limitations of equipment used in classes within the Ocean Engineering department at Florida Institute of Technology. By meeting with several stakeholders and end users, a series of stakeholder requirements, capabilities, and component-level requirements were de- veloped that informed the design constraints. There were several hardware iterations of the board, culminating in Revision F5 which was extensively tested and proven. The design was inspected after testing concluded to determine which capabilities …

Assessing The Thermodynamic Potential Of Deep Eutectic Solvents In The Absorption Of Greenhouse Gases Through Computational Methods., Thomas Michael Quaid

Theses and Dissertations

Greenhouse gas (GHG) capture is a fundamental technology in the fight against climate change. Some species of devastating GHGs like fluorinated compounds are released directly to the air from industrial processes. Some GHGs are co-produced in sustainably derived biofuels which require absorptive upgrading. The development of more efficient, cost effective, and environmentally friendly methods of capturing GHG’s from pollutant sources and industrial streams is of utmost importance in the fight against climate change. Deep eutectic solvents have entered the separations stage as potential disruptors to conventional solvent choices for a variety of applications. Deep eutectic solvents (DES) are compounds of …

Band Motor Crankshaft For Hydrogen Combustion Engine, Lincoln Hofer, Jacqueline Mendoza Cortes, Austin Rhee, Roze Raymond

Mechanical Engineering

This report outlines the steps in which a group of four mechanical engineers at California Polytechnic State University California, San Luis Obispo, addressed an issue brought up by Professor Roger Benham from the Materials Department. The task included developing an automated and modular testing platform for a revolutionary concept crankshaft prototype – meant for a hydrogen combustion engine - developed and produced by the sponsor: Roger Benham. A testing platform is necessary to measure the performance of the shaft design created to eventually implement this subsystem into a complete hydrogen engine design. Values being tested include geometric validation, maximum static …

Synthesis Of Glucomannan-Xylan Composite Biofilms: Characterization And Applications For Food Packaging, Kholoud Al-Ajlouni

Dissertations

Scientists are most concerned about the need for green alternatives for efficient food packaging polymers to replace petroleum-based packaging products. The waste of corn cobs and the roots of the konjac plant contain hemicellulose, xylan and glucomannan respectively, and can be used to produce biofilms for food packaging.

The aim of these studies is to formulate biofilms from glucomannan-xylan blends that compete with petroleum-based plastic wrap. The biofilms must be strong, smooth, transparent, have a water vapor barrier, have the required mechanical properties and be cheap. Biofilms from glucomannan were stiff, lacked mechanical properties, and absorbed moisture. Therefore, it reacts …

Structural Characterization Of Cardiomyocyte Microenvironment In Murine Myocardium By Multimodal Stimulated Emission Depletion Microscopy, Zhao Zhang

All Dissertations

Cardiomyopathy, a disease of the heart muscle, is characterized by structural and functional abnormalities of the myocardium, in the absence of coronary artery disease, hypertension, valvular disease or congenital heart disease. In 2020, there were approximately 6.11 million prevalent cases of cardiomyopathy and myocarditis, and 0.37 million related deaths worldwide. Many affected individuals are asymptomatic, and chronically treated patients are at risk of heart failure. Within the myocardium, cardiomyocytes reside in a complex and dynamic extracellular matrix (ECM), consisting of basement membrane and interstitial matrix. The interactions between cardiomyocytes and myocardial ECM play a critical role in maintaining cardiac geometry …

A Digital Triplet For Utilizing Offline Environments To Train Condition Monitoring Systems For Rolling Element Bearings, Ethan Wescoat

All Dissertations

Manufacturing competitiveness is related to making a quality product while incurring the lowest costs. Unexpected downtime caused by equipment failure negatively impacts manufacturing competitiveness due to the ensuing defects and delays caused by the downtime. Manufacturers have adopted condition monitoring (CM) techniques to reduce unexpected downtime to augment maintenance strategies. The CM adoption has transitioned maintenance from Breakdown Maintenance (BM) to Condition-Based Maintenance (CbM) to anticipate impending failures and provide maintenance actions before equipment failure. CbM is the umbrella term for maintenance strategies that use condition monitoring techniques such as Preventive Maintenance (PM) and Predictive Maintenance (PdM). Preventive Maintenance involves …

Engineering Next-Generation Cardiovascular Therapies, Ryan Walker Barrs

All Dissertations

Cardiovascular diseases are the leading cause of death worldwide. Heart disease, in particular, accounted for 1 in every 5 deaths in the United States in 2021, representing a substantial public health burden that will require more effective treatment options to alleviate. Heart transplant surgery is currently the only option to completely restore cardiac function, but many patients die while waiting for a transplant due to a longstanding organ donor shortage. Thus, there is a dire need for accessible treatment options that can repair damaged hearts. Recent advances in tissue engineering and regenerative medicine have accelerated the development of therapies for …

Experimental And Computational Platforms For Studying Systems Mechanobiology, Brendyn Miller

All Dissertations

Mechanical stimulation through physical activity has been shown to play an important role in treating and preventing several non-communicable diseases such as hypertension, lower back pain (LBP), type-2 diabetes mellitus, and several cancers. This is accomplished through the regulation of cellular behavior and tissue remodeling within the body at both the micro- and macro-scale levels. The goal of mechanobiology research is to gain in-depth knowledge and understanding of how cells sense physical forces in conjunction with other biochemical cues and translate those factors into important biological functions that either maintain tissue homeostasis or lead to pathological states. Understanding these processes …

Addressing Mercury Contamination From Artisanal Gold Mining In Colombia: Pollution Exposure, Health Risk, And Educational Efforts In The Communities Of La Toma, Colombia, Kelli Mccourt

All Dissertations

Artisanal and small-scale gold mining (ASGM) has become a primary environmental and public health concern in the region of Colombia known as Alto Cauca. The predominantly Afro-descendent communities of Yolombó and La Toma in Alto Cauca have experienced pollution linked to the use of mercury in the ASGM process. For the past decade, mercury has emerged as a contaminant of increasing concern in the communities due to its toxicity and ability to bioaccumulate and biomagnify. Likewise, mining in the region raises concerns over the leaching of metals into local waters. Given the complexity of risk and pollution in the communities, …

Influence Of Photoreactor Design Parameters On The Performance Of Ultraviolet Light-Induced Degradation Of Per-And Polyfluoroalkyl Substances By Bismuth Oxy Hydroxy Phosphate And Hexagonal Boron Nitride Photocatalystsinfluence Of Photoreactor Design Parameters On The Performance Of Ultraviolet Light-Induced Degradation Of Per-And Polyfluoroalkyl Substances By Bismuth Oxy Hydroxy Phosphate And Hexagonal Boron Nitride Photocatalysts, Mojtaba Qanbarzadeh

All Dissertations

The main objective of this study is to evaluate the practical applicability of heterogeneous photocatalysis technology powered by ultraviolet (UV) photons for the degradation of per and -polyfluoroalkyl substances (PFAS) in contaminated groundwater samples. For this purpose, bismuth oxyhydroxy phosphate (BOHP) and hexagonal boron nitride (hBN) were utilized as the main photocatalysts in slurry photodegradation systems. BOHP (Bi₃O(OH)(PO₄)₂) is a wide bandgap semiconductor with high activity for degrading long-chain perfluorocarboxylic acids (PFCAs) in mild operational conditions. Boron nitride (BN) is another wide bandgap semiconductor material that was used in this study due to its …

Citrate Hydrogels For Regenerative Medicine Applications, Mark Pitman

All Dissertations

Regenerative medicine focuses on restoring cells and tissues to re-establish proper function. Hydrogels are a customizable and adaptable set of materials that can be engineered as tools for regenerative medicine. My research has focused on developing and applying biomaterials to spinal cord injury and chronic wounds. Spinal cord injury and chronic wounds share pathophysiological issues with oxidative stress, excess free, and macrophage polarization. Furthermore, both conditions lack robust, effective treatments. We hypothesized that citrate hydrogels – hydrogels that contain citric acid as a functional component – would provide radical scavenging and iron chelation that would benefit both conditions. The hydrogels …

Gravel Conveyance System: An Evaluation On E. Coli Removal Capabilities, Madison Grace

Graduate Theses and Dissertations (2019 - present)

In many rural, low-income communities such as the Black Belt of Alabama, there is a lack of wastewater infrastructure and solutions leaving homeowners non-compliant to state and federal law and with potential health risks on their property. New regulations have allowed for onsite, surface discharge for single family homes that are not connected to a centralized sewer or large flow development. These new regulations also contain strict disinfection criteria for the treated wastewater that will be discharged. A low-cost and low-maintenance treatment system that provides adequate disinfection is necessary to allow homeowners to take advantage of the new opportunity for …

An Analysis On Wildfire Mitigations Employed By Utilities In California, Evan Luu

Master's Theses

As climate change continues to worsen, environmental effects are felt by many people around the world. In California, some of its most damaging wildfires have been found to be started by utilities. As the state continues to suffer from worsening wildfire conditions, the utilities need to implement a variety of wildfire mitigations to help reduce the risk of wildfires that can affect the state and its residents. This paper analyzes the effectiveness of four mitigations employed across three California utilities and suggests potential ways for the mitigations to be used together. The technologies evaluated are covered conductor, rapid earth fault …

Classification Of Large Scale Fish Dataset By Deep Neural Networks, Priyanka Adapa

Electronic Theses, Projects, and Dissertations

The development of robust and efficient fish classification systems has become essential to preventing the rapid depletion of aquatic resources and building conservation strategies. A deep learning approach is proposed here for the automated classification of fish species from underwater images. The proposed methodology leverages state-of-the-art deep neural networks by applying the compact convolutional transformer (CCT) architecture, which is famous for faster training and lower computational cost. In CCT, data augmentation techniques are employed to enhance the variability of the training data, reducing overfitting and improving generalization. The preliminary outcomes of our proposed method demonstrate a promising accuracy level of …

Rabrs For Use In Biologically Enhanced Precipitation Of Struvite In Anaerobic Digester Effluent (Municipal Wastewater), Parker R. Goldsberry

All Graduate Theses and Dissertations, Fall 2023 to Present

Rotating Algae Biofilm Reactor (RABR) technology has been researched in studies over the past decade directed at nutrient management for water resource recovery facilities (WRRFs). This study investigated the growth of slow-release fertilizer crystals, referred to as struvite, in the algae biofilms of Rotating Algae Biofilm Reactors (RABRs) at the Central Valley Water Reclamation Facility, the largest water resource recovery facility in the State of Utah. RABRs used anaerobic digester (AD) filtrate as their nutrient source. AD effluent is high in nutrients like nitrogen and phosphorus. The levels of both phosphorus and nitrogen (in the form of ammonia) are regulated …

Effects Of Indole Derivative Idc16 On Cell Proliferation, Transfection, And Alternative Splicing In Production Of Lentiviral Vectors By Transient Co-Transfection, Nataly Mier

All Graduate Theses and Dissertations, Fall 2023 to Present

Viruses can efficiently introduce genetic material into a variety of cells through cell infection. Therefore, viral vectors, defined as virus-based gene delivery systems, effectively transfer functional gene constructs to target cells. Among different viral vectors, lentiviral vectors are of interest due to the advantages offered to research and therapeutic applications. Low productivity of lentiviral vectors in mammalian producer cells and high production cost, however, remain barriers to the vectors’ routinary use, particularly in clinical settings.

Since most lentiviral vectors are derived from human immunodeficiency virus type I, their genome has been engineered to increase safety. Nevertheless, modifications of the lentiviral …

Upcyclying Of Polyethylene Terephtalate By Addition Of Thermoplastic Elastomer, Diego Francisco Bermudez

Open Access Theses & Dissertations

Continual overconsumption of single-use plastics has generated challenges of solid waste management across the United States. Common plastic waste management solutions, such as landfill, have caused the migration of contaminants into the environment consequently affecting not only the health of wildlife, but also that of human beings. Alternative strategies for the handling of single-use plastic such as polyethylene terephthalate (PET), used in the food packaging industry, can ultimately help mitigate the noxious consequences of single-use plastics affecting entire ecosystems. This study demonstrates a potential avenue of materials upcycling by studying the effects of coupling PET with the thermoplastic elastomer styrene-ethylene-butylene-styrene …

An Optimization Procedure To Design Nozzle Contours For Hypersonic Wind Tunnels, Omar Antonio Dominguez

Open Access Theses & Dissertations

Supersonic wind tunnels allow scientists and researchers to evaluate and analyze the behaviors of objects under real-life conditions when subjected to supersonic speeds. One of the main complexities when building a wind tunnel is the design of the convergent-divergent nozzle that is used to produce high-speed and high-quality flows. To achieve supersonic speeds, this nozzle adopts a specialized approach that incorporates the complexities of flow compressibility. The compressible effect is accurately evaluated using isentropic relations, allowing for precise determination of stagnation pressure and temperature, and static pressure and temperature relevant to the desired Mach number. Isentropic equations used to define …

Tissue-Engineered Models For Investigating Nerve Infiltration In Fibrotic Microenvironments, Nikolas Ala-Kokko

Graduate Theses and Dissertations

Neuron infiltration is involved in a multitude of diseased states and is critical in the regeneration of physiologic conditions. Modeling of innervation in three-dimensional, heterogenous, environments would allow early investigation in the crosstalk of neurons and native cells in normal and pathophysiologic microenvironments to gain an understanding of mechanistic functions. This would allow for greater insight into the role of metabolism, cell adhesion, microenvironmental alternations, cellular crosstalk, and signaling pathways that play a role in the promotion and prevention of neurite growth. In this thesis, with the construction of collagen-based hydrogels as the foundation for cell culture, we investigate cell …

An Integrated Neutrosophic Approach For The Urban Energy Internet Assessment Under Sustainability Dimensions, Walaa M. Sabry

Neutrosophic Systems with Applications

The urgent issue of climate change has resulted in an increasing preoccupation with the transition to low-carbon energy. The urban energy internet (UEI) enables the efficient utilization of renewable energy via the integration of contemporary energy grids, smart energy services, and cyber-physical systems. Consequently, the assessment of urban energy is vital for the construction of low-carbon cities. This study intends to provide an integrated decision-making approach for addressing assessment challenges related to UEI, with a focus on sustainability. The introduced approach adopts the opinions of three experts to express their opinions through semantic terms in evaluating sustainability factors and evaluating …

Experimental Analysis Of Nonlinear Wave Propagation In Bistable Mechanical Metamaterials With A Defect, Samuel R. Harre

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

Mechanical metamaterials built up of compliant units can support the propagation of linear and nonlinear waves. A popular architecture consists of a one-dimensional chain of bistable elements connected by linear springs. This type of chain can support nonlinear transition waves that switch each element from one stable state to the other as they propagate along the chain. One way to manipulate the propagation of such waves is via introduction of a local inhomogeneity, i.e., a defect in the otherwise periodic chain. Recent analytical and numerical work has shown that based on its initial velocity, a transition wave may be reflected, …

Assessing Chondrocyte Viability Of Articular Cartilage Using Label-Free Imaging Methods, Hongming Fan

All Dissertations

ABSTRACT

Articular cartilage covers the ends of bones, providing a protective layer that can endure forces during movement. Chondrocytes, the only cell type found in articular cartilage, produce collagen fibers and proteoglycans that maintain the extracellular matrix (ECM). Cartilage damage impairs chondrocytes and their ability to maintain tissue stability. There are various factors that can cause cell death, which can be broadly classified into two categories: programmed cell death (apoptosis) and non-programmed cell death (necrosis). The consequences of cell death can have significant impacts on the extracellular matrix (ECM). The ECM is a complex network of proteins and carbohydrates that …

An In Vitro Platform To Characterize Myocardial Wound Remodeling, Jonathan Heywood

All Dissertations

Heart Failure, sometimes called Congestive Heart Failure, is a collection of pathological disruptions in which the heart is unable to pump blood properly. It affects over 6 million people in the United States, with the cost-per-patient at approximately $30,000 and the total cost estimated to be $160 billion by 2030. There is no single cause of heart failure, as it can manifest as a varying array of diseases, disorders, and syndromes. However, many of these underlying diseases result specifically from dysregulation of mechanically active cells called cardiac fibroblasts, which play a critical role in the remodeling of the extracellular matrix …

Evaluating Differences In Optical Properties Of Indolent And Aggressive Murine Breast Tumors Using Quantitative Diffuse Reflectance Spectroscopy, Joel Rodriguez Troncoso, Umme Marium Mim, Jesse D. Ivers, Santosh K. Paidi, Mason G. Harper, Khue G. Nguyen, Sruthi Ravindranathan, Lisa Rebello, David E. Lee, David A. Zaharoff, Ishan Barman, Narasimhan Rajaram

Biomedical Engineering Faculty Publications and Presentations

We used diffuse reflectance spectroscopy to quantify tissue absorption and scattering-based parameters in similarly sized tumors derived from a panel of four isogenic murine breast cancer cell lines (4T1, 4T07, 168FARN, 67NR) that are each capable of accomplishing different steps of the invasion-metastasis cascade. We found lower tissue scattering, increased hemoglobin concentration, and lower vascular oxygenation in indolent 67NR tumors incapable of metastasis compared with aggressive 4T1 tumors capable of metastasis. Supervised learning statistical approaches were able to accurately differentiate between tumor groups and classify tumors according to their ability to accomplish each step of the invasion-metastasis cascade. We investigated …