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Development Of Quaternized Poly(Vinyl Alcohol)/Chitosan/Two-Dimensional Molybdenum Disulfide Anion Exchange Membrane As A Separator For Electrochemical Processes, Nuttapon Suppanucroa

Chulalongkorn University Theses and Dissertations (Chula ETD)

In response to the global shift toward the expansion of large-scale energy storage systems (ESSs), secondary zinc-air batteries (ZABs) have attracted significant attention as promising candidates for next-generation batteries due to their high energy density, cost-effectiveness, and safety. However, their long-term practical application is hindered by several challenges associated with the membrane separator, including the formation of resistive zinc oxide layers and low hydroxide-ion conductivity. These limitations are attributed to poor ion-selectivity, which impedes electrochemical reactions and degrades battery performance. To address these issues, this study developed a series of innovative membrane separators featuring quaternary ammonium (QA)-functionalized polyvinyl alcohol (PVA), …

ผลกระทบของไมโครพลาสติกต่อประสิทธิภาพการทำงานของระบบบำบัดน้ำชะขยะมูลฝอยและจุลินทรีย์ในระบบ, ธนวัฒน์ พงษ์ทองเจริญ

Chulalongkorn University Theses and Dissertations (Chula ETD)

งานวิจัยนี้เป็นงานวิจัยเพื่อศึกษาผลกระทบของไมโครพลาสติกต่อประสิทธิภาพการทำงานของระบบบำบัดน้ำชะขยะมูลฝอยและจุลินทรีย์ในระบบโดยใช้ระบบบำบัดจำลองแบบเอสบีอาร์ในการทดลองครั้งนี้ โดยงานวิจัยครั้งนี้มีจุดประสงค์เพื่อ 1.ศึกษาผลกระทบของไมโครพลาสติกต่อประสิทธิภาพการกำจัดซีโอดีและไนโตรเจนของระบบบำบัดแบบเอสบีอาร์ 2. เพื่อศึกษาผลกระทบของไมโครพลาสติกต่อการเปลี่ยนแปลงขององค์ประกอบชุมชนจุลินทรีย์ในระบบบำบัดแบบเอสบีอาร์ และ 3. เพื่อศึกษาเส้นทางของไมโครพลาสติกในระบบและความสามารถในการกำจัดไมโครพลาสติกผ่านระบบบำบัดน้ำเสียแบบแบบเอสบีอาร์ โดยงานวิจัยนี้ได้ทำการทดลองดำเนินระบบบำบัดน้ำเสียจำลองแบบเอสบีอาร์เป็นจำนวน 3 ปฏิกรณ์ที่ใส่ไมโครพลาสติกในปริมาณที่แตกต่างกันคือ 0 มก./ล., 10 มก./ล. และ 1,000 มก./ล. ดำเนินระบบเป็นระยะเวลา 94 วัน พบว่าประสิทธิภาพของการบำบัดซีโอดีโดยเฉลี่ยของทั้งสามระบบคือ 61.5%, 57.9% และ 50.8% ตามลำดับซึ่งประสิทธิภาพของการบำบัดซีโอดีลดลงอย่างมีนัยยะสำคัญเมื่อไมโครพลาสติกในระบบเพิ่มขึ้น เช่นเดียวกับพีเอชที่แปรผกผันกับปริมาณไมโครพลาสติกและความสัมพันธ์นี้ยังสอดคล้องกับสัดส่วนของชุมชนจุลินทรีย์ที่แตกต่างกันในแต่ละระบบอีกด้วย แต่ยังไม่สามารถสรุปได้ว่าปริมาณไมโครพลาสติกที่เพิ่มขึ้นนั้นส่งผลกลับประสิทธิภาพการบำบัดไนโตรเจนและฟอสฟอรัสได้ นอกจากไมโครพลาสติกที่เพิ่มขึ้นจะส่งผลให้ประสิทธิภาพของระบบบำบัดน้ำเสียแบบเอสบีอาร์ลดลงแล้วไมโครพลาสติกยังสามารถสะสมตกค้างในระบบและมีบางส่วนสามารถออกจากระบบบำบัดไปสู่สิ่งแวดล้อมได้ ดังนั้นแล้วระบบบำบัดที่ต้องรับน้ำเสียที่มีไมโครพลาสติกเจือปนเยอะเช่นน้ำชะขยะควรจะมีการกำจัดไมโครพลาสติกก่อนเข้าระบบ

Hydrogen Production From Glycerol Using Sorption Enhanced Chemical Looping Steam Reforming With Multifunctional Pellet Catalyst, Talita Nimmas

Chulalongkorn University Theses and Dissertations (Chula ETD)

In this study, the synthesis of a pellet-scale material for hydrogen (H₂) production from glycerol via the Sorption Enhanced Chemical Looping Steam Reforming (SE-CL-SGR) process was investigated. The research focuses on material synthesis, performance enhancement, and the economic and environmental advantages of the developed material. Among the synthesis methods compared, the Stepwise Calcination process demonstrated the best performance, producing structurally stable and mechanically robust pellets. The optimal CaO/support mass ratio for the Ni-supported material (15Ni(70Ca.30Support)) was found to achieve 90% v/v H₂ purity with a pre-breakthrough time of approximately 75 minutes. Rare-earth metal oxides (CeO₂, La₂O₃) addition also improved mechanical …

Development Of A Forest Fire Detection Device Based On Agent-Based Simulation, Teerapat Tappanom

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study had three main objectives: to identify the factors influencing forest fire spread, to design and develop a forest fire spread model, and to design and develop a forest fire detection device. The researcher utilized scatter plots, correlation analysis, and multiple linear regression analysis based on climatic data collected from the Chiang Mai Meteorological Station between 1998 and 2021 to investigate the parameters affecting fire spread. A forest fire spread model was developed using an Agent-Based Model (ABM) in AnyLogic 8.8.3 Personal Learning Edition to estimate the burned area under different scenarios with wind directions set to the north …

Analysis Of Shoreline Erosion At Cumberland Island Using Advanced Circulation (Adcirc) Modeling, Christopher Harrigan

UNF Graduate Theses and Dissertations

This study investigates shoreline erosion at Brickhill Bluff along the Brickhill River in Cumberland Island National Seashore using ADCIRC. Two scenarios were modeled during this research: bed stresses during regular tidal cycles and bed stresses during worst-case storm (i.e., hurricane) conditions. Velocities from these models were used to compute bed shear stresses and these stresses were compared to site’s sediment critical shear stress. Results appear to show that a meander in the Brickhill River causes relatively high (i.e., on the order of 0.27 ��/��² ) shear stresses during regular tidal cycles and even higher (i.e., on the order of …

A Simple, Inexpensive Alkaline Method For Bacterial Dna Extraction From Environmental Samples For Pcr Surveillance And Microbiome Analyses, Abdulkarim Shwani, Bin Zuo, Adnan Alrubaye, Jiangchao Zhao, Douglas D. Rhoads

Poultry Science Faculty Publications and Presentations

DNA extraction for downstream molecular diagnostic applications can be an expensive, time-consuming process. We devised a method to quickly extract total bacterial DNA from environmental samples based on the sodium hydroxide lysis of cells with or without capture by magnetic beads for subsequent PCR or quantitative PCR. The final DNA extraction method using NaOH is extremely low-cost and can be completed in as little as 10 min at room temperature with dilution, or the DNA can be further purified using silica-coated paramagnetic beads. NaOH extraction was effective for Gram-negative and Gram-positive bacteria in samples from air, soil, sewage, food, laboratory …

Barriers And Variable Spacing Enhance Convective Cooling And Increase Power Output In Solar Pv Plants, Brooke Stanislawski, Todd Harman, Raul Bayoan Cal, Marc Calaf

Mechanical and Materials Engineering Faculty Publications and Presentations

When the temperature of solar photovoltaic (PV) modules rises, efficiency drops and module degradation accelerates. Thus, it is beneficial to reduce module operating temperatures. Previous studies of solar power plants have illustrated that incoming flow characteristics, turbulent mixing, and array geometry can strongly impact convective cooling, as measured by the convective heat transfer coefficient h. In the fields of heat transfer and plant canopy flow, previous work has shown that system-scale arrangement modifications—e.g., variable spacing, barriers, or windbreaks—can passively alter the flow, enhance turbulent mixing, and influence convection. However, researchers have not yet explored how variable spacing or barriers …

Detection Of Tooth Position By Yolov4 And Various Dental Problems Based On Cnn With Bitewing Radiograph, Kuo Chen Li, Yi-Cheng Mao, Mu-Feng Lin, Yi-Qian Li, Chiung-An Chen, Tsung-Yi Chen, Patricia Angela R. Abu

Department of Information Systems & Computer Science Faculty Publications

Periodontitis is a high prevalence dental disease caused by bacterial infection of the bone that surrounds the tooth. Early detection and precision treatment can prevent more severe symptoms such as tooth loss. Traditionally, periodontal disease is identified and labeled manually by dental professionals. The task requires expertise and extensive experience, and it is highly repetitive and time-consuming. The aim of this study is to explore the application of AI in the field of dental medicine. With the inherent learning capabilities, AI exhibits remarkable proficiency in processing extensive datasets and effectively managing repetitive tasks. This is particularly advantageous in professions demanding …

Improveing F-Beta Score In Classifying Shark Data Into Shark Behaviors, Ibrahim M. Ali

CGU Theses & Dissertations

One metric used to measure classification performance in machine learning is F-beta score. The objective in this thesis is to improve the average F-b score computed in classifying shark data into shark behaviors, namely; Resting, Swimming, Feeding, and Non-Directed Motion (NDM). Synthetic Minority Oversampling Technique (SMOTE) and Adaptive Synthetic Sampling (ADASYN) are utilized to balance the data, from which pre-processed Fast Fourier Transform (FFT), Walsh-Hadamard Transform (WHT), and Autocorrelation (AC) features are extracted then classified using Convolutional Neural Network (CNN) and K-Nearest Neighbors (K-NN). All the combinations of the two balancing techniques, the three feature types, and the two machine …

Type-B Energetic Processes: Their Identification And Implications, James Weifu Lee

Chemistry & Biochemistry Faculty Publications

We have now identified two thermodynamically distinct types (A and B) of energetic processes naturally occurring on Earth. Type-A energy processes, such as classical heat engines, apparently well follow the second law of thermodynamics; Type-B energy processes, such as the newly discovered thermotrophic function that isothermally utilizes environmental heat energy to perform useful work in driving ATP synthesis, follow the first law of thermodynamics (conservation of mass and energy) but do not have to be constrained by the second law, owing to their special asymmetric functions. Several Type-B energy processes such as asymmetric function-gated isothermal electricity production and epicatalysis have …

Photocatalytic Efficiency Of Graphene Quantum Dots Incorporated With Tio2 Under Visible Light For Degradation Of Organic Dye, Ilada Sanprasert

Chulalongkorn University Theses and Dissertations (Chula ETD)

The rapid industrialization and increased use of organic dyes for production in many industries have led to extensive environmental pollution concerns. At present, photocatalytic dye degradation is an outstanding environmentally friendly process for removal and treatment of organic dyes from water pollutants that cause no harm to the environment. In this research, we investigated the performance of different crystallographic structure of TiO2 (anatase, rutile, and mixed anatase/rutile P25 TiO2) under visible light irradiation. Furthermore, the TiO2 was modified using graphene quantum dots to evaluate their effect on photocatalytic efficiency. The characterization results of the GQDs/TiO2 photocatalyst composites demonstrated that GQDs …

Developing An Immunomodulatory Strategy Using Biophysical Cues To Modulate Macrophage Phenotype For Fracture Healing And Bone Regeneration, Harshini Suresh Kumar

Theses and Dissertations--Biomedical Engineering

Chronic inflammation is a major cause of the pathogenesis of musculoskeletal diseases such as fragility, fracture, and nonunion. Studies have shown that modulating the immune phenotype of macrophages from proinflammatory to pro-healing can heal recalcitrant bone defects. Current therapeutic strategies predominantly apply biochemical cues, which often lack target specificity, and controlling their release kinetics in vivo is challenging spatially and temporally. We have developed a magnetic iron-oxide nanocomplexes (MNC)-based therapy for resolving chronic inflammation in the context of promoting fracture healing. Here, we show that MNC internalized macrophages, when coupled with an external magnetic field, can exert an intracellular magnetic …

Relating Temperature, Sleep, And Pathology In Mouse Models Of Alzheimer's Disease, Jun Wang

Theses and Dissertations--Biomedical Engineering

It is well established that sleep plays an important role in Alzheimer’s disease (AD) pathology. There is growing evidence that sleep issues may accelerate AD pathology by interrupting the physiological clearance of amyloid‐β (Aβ) from the brain, which mainly occurs in normal sleep. As the over-accumulation of amyloid‐β (Aβ) is believed to lead the cascade effect that eventually causes neurodegeneration in AD patients, development of therapeutic strategies for improving sleep quality may help slow disease progression. It is well documented that the composition and dynamics of sleep are sensitive to changes in ambient temperature. We therefore developed a sleep enhancement …

Development Of Iron Oxide Nanozyme For Cancer Therapy, Zhongchao Yi

Theses and Dissertations--Biomedical Engineering

Cancer therapies that leverage reactive oxygen species (ROS) have shown potential for cancer inhibition due to their ability to induce oxidative stress in tumor cells. Iron oxide nanoparticles (IONPs) have garnered significant attention as a promising platform for ROS-dependent disease treatment, owing to their remarkable biocompatibility and Fenton catalytic activity. However, the low catalytic activity of IONPs has been a major hurdle in their clinical translation. To overcome this challenge, IONPs of different compositions are examined for their Fenton reaction under pharmacologically relevant conditions in this work. The results show that wüstite (Fe_1-xO) nanoparticles exhibit higher catalytic activity …

Multiscale Fiber Remodeling In The Heart Using A Finite Element Framework, Mohammad Mehri

Theses and Dissertations--Mechanical and Aerospace Engineering

The organization of myofibers and extra cellular matrix within the myocardium plays a significant role in defining cardiac function. When pathological events occur, such as myocardial infarction (MI) or hypertrophic cardiomyopathy (HCM), this organization can become disrupted, leading to alteration in pumping performance. The current study proposes a multiscale finite element (FE) framework to determine realistic fiber distributions in the left ventricle (LV). This is achieved by implementing a stress-based fiber reorientation law, which seeks to align the fibers with local traction vectors, such that contractile force and load bearing capabilities are maximized. By utilizing the total stress (passive and …

Development Of Lignin Derived Hydrophobic Deep Eutectic Solvents As Sustainable Solvents And Their Applications, Yuxuan Zhang

Theses and Dissertations--Biosystems and Agricultural Engineering

The development of green solvents is a pivotal endeavor in achieving the goals of green chemistry. Ionic liquids (ILs) and deep eutectic solvents (DESs) have emerged as promising green alternatives to conventional organic solvents due to their low toxicity, potential biodegradability, low volatility, and high tunability. Compared to ILs, DESs have attracted more attention recently as they are easier and less expensive to produce. Hydrophobic DESs (HDESs), a new generation of DESs introduced in 2015, have gained considerable interest for their ability to overcome the instability issues of traditional DESs when exposed to water. This advancement extensively broadens the application …

The Characterization, Assessment, And Shear Strength Of Turfgrass Soil In North American Thoroughbred Racing, Peter Schmitt

Theses and Dissertations--Biosystems and Agricultural Engineering

Minimizing catastrophic injuries to racehorses, which also protects the riders, is critical for the future of the Thoroughbred racing industry. While the causes of catastrophic injuries are multifactorial, the condition of the racing surface is one of only a few factors that affects all horses in competition. Horse racing surfaces must retain enough shear strength to support the hoof of a Thoroughbred at a gallop. Turfgrass racing surfaces also require healthy turf to reinforce the footing while also achieving a high infiltration rate to allow races to run on the turf soon after or even during rain. This research is …

The Efficacy Of Wetland Treatment Systems Used To Treat Runoff Mixtures From Different Landscapes Across Kentucky, Emily Nottingham

Theses and Dissertations--Biosystems and Agricultural Engineering

The overarching goal was to improve our understanding of the cumulative effect of increased use and occurrence of commonly detected contaminants of potential concern (CPCs) on nitrogen (N) transformation processes in wetlands. The central hypothesis was that those various mixtures of nutrients and CPCs entering treatment wetlands impact N removal pathways. The first study assessed the current state of the literature on the implications to N removal in wetlands in the presence of pesticides and antibiotics. 181 primary studies were identified. Removal efficiencies for nitrate varied widely across the studies, with CPCs impacting microbial communities. A knowledge gap remains in …

Valorization Of Bourbon Distilling Waste Using Thermal Conversion Processes For Nutrient-Rich Food Products, Tosin Olayemi Olanrewaju

Theses and Dissertations--Biosystems and Agricultural Engineering

The Commonwealth of Kentucky accounts for over 95% of global bourbon production. A primary byproduct of bourbon production is stillage, which has a very high moisture content (91–94%) and high biochemical oxygen demand, making it challenging to manage. The recent expansion of the bourbon industry has heightened environmental concerns regarding the best methods to handle this byproduct. Dewatering and drying of stillage produce distillers' spent grains (DSG-B), which are rich in dietary fiber and protein and could serve as an excellent source of functional fiber in human nutrition.

This study examined the approach for dewatering, drying, inclusion of DSG-B as …

Development Of A Low-Cost Resistivity Meter For Local Groundwater Survey, Jose Claro N. Monje, Ma. Leonora Guico, Carlos M. Oppus, Mark Glenn F. Retirado, Alfonso Rafael Cuezon, Justin Bryce M. Torres

Electronics, Computer, and Communications Engineering Faculty Publications

The researchers developed a low-cost resistivity meter with a built-in data file storage system using locally available parts. This effort was done in partnership with the National Water Resources Board and the Department of Science and Technology under the Water for Tourism project for water quality monitoring in tourist areas in the Philippines such as Boracay and Caticlan. The resistivity meter system consists of a power supply module, a sensor module, and an equipment module, built using locally available materials and equipment, emulating the commercially available PASI model 16 GL-N. Due to pandemic restrictions, the developed prototype could only be …

Methods That Support The Validation Of Agent-Based Models: An Overview And Discussion, Andrew Collins, Matthew Koehler, Christopher Lynch

Engineering Management & Systems Engineering Faculty Publications

Validation is the process of determining if a model adequately represents the system under study for the model’s intended purpose. Validation is a critical component in building the credibility of a simulation model with its end-users. Effectively conducting validation can be a daunting task for both novice and experienced simulation developers. Further compounding the difficult task of conducting validation is that there is no universally accepted approach for assessing a simulation. These challenges are particularly relevant to the paradigm of Agent-Based Modeling and Simulation (ABMS) because of the complexity found in these models’ mechanisms and in the real-world situations they …

Nanosecond Pulsed Electric Fields Increase Antibiotic Susceptibility In Methicillin-Resistant Staphylococcus Aureus, Alexandra E. Chittams-Miles, Areej Malik, Erin B. Purcell, Claudia Muratori

Bioelectrics Publications

Staphylococcus aureus is the leading cause of skin and soft-tissue infections (SSTIs). SSTIs caused by bacteria resistant to antimicrobials, such as methicillin-resistant S. aureus (MRSA), are increasing in incidence and have led to higher rates of hospitalization. In this study, we measured MRSA inactivation by nanosecond pulsed electric fields (nsPEF), a promising new cell ablation technology. Our results show that treatment with 120 pulses of 600 ns duration (28 kV/cm, 1 Hz), caused modest inactivation, indicating cellular damage. We anticipated that the perturbation created by nsPEF could increase antibiotic efficacy if nsPEF were applied as a co-treatment. To test this …

Rapid Expansion Of The Limpkin Aramus Guarauna Across Anglo-America, A. Alam, R. Kaddoura, J. Jackson, R. Kannan

Journal of the Arkansas Academy of Science

We quantitatively document and describe the recent rapid expansion of the Limpkin Aramus guarauna across Anglo-America (United States and Canada). Using data downloaded from eBird (www.eBird.org), we present graphs showing the explosive growth in reports of number of individual birds and frequencies in checklists for this species in several parts of Anglo- America outside Florida (its traditional Anglo- American range). We also provide geographic progression maps to illustrate the expansion through time (2013-2023) and furnish qualitative information on foods consumed by the bird in new areas, based on a survey of 274 images uploaded by citizen scientists in eBird. Possible …

Energy Content Of Acorns Of Shinnery Oak (Quercus Havardii) In The Diet Of Scaled Quail (Callipepla Squamata) In Southeastern New Mexico, J.L. Hunt, M.E. Grilliot, T.L. Best, D. Lozano-Lopez, E.R. Neilson, I.C. Castillo

Journal of the Arkansas Academy of Science

Shinnery oak (Quercus havardii) is a deciduous, low-growing shrubby tree that is a dominant plant in large areas of grassland in western Oklahoma, the Texas Panhandle, and eastern New Mexico. Acorns of the plant are important food items for many wildlife species, including scaled quail (Callipepla squamata), mourning doves (Zenaida macroura), and endangered lesser prairie-chickens (Tympanuchus pallidicinctus). We analyzed the energy content of acorns of shinnery oak obtained from the crops of scaled quail collected from plains-mesa sand-scrub in Lea and Eddy counties, New Mexico. Acorns were removed from crops and dried for 48 hours at 60°C to remove moisture …

Machine Learning Applications In Spine Biomechanics, Farshid Ghezelbash, Amir Hossein Eskandari, Xavier Robert-Lachaine, Shufan Cao, Mehran Pesteie, Zhuohua Qiao, Aboulfazl Shirazi-Adl, Christian Larivière

Études primaires

Spine biomechanics is at a transformation with the advent and integration of machine learning and computer vision technologies. These novel techniques facilitate the estimation of 3D body shapes, anthropometrics, and kinematics from as simple as a single-camera image, making them more accessible and practical for a diverse range of applications. This study introduces a framework that merges these methodologies with traditional musculoskeletal modeling, enabling comprehensive analysis of spinal biomechanics during complex activities from a single camera. The real-world applications explored in this study include assessment in workplace lifting, evaluation of whiplash injuries in car accidents, and biomechanical analysis in professional …

Groundwater Movement In Boiling Spring, Onyx Spring, Yelton Spring, Wilkins Spring, Hudgens Spring And Natural Bridge Spring, Katherin Roxana Montes Chamorro

Masters Theses

"Onyx Spring, Boiling Spring, Yelton Spring, Wilkins Spring, Hudgens Spring, and Natural Spring are located on public land in Pulaski and Phelps County, Missouri. They were selected randomly to determine groundwater movement and compare water chemistry from the springs that are connected.

Using a dye tracing method, we were able to determine the direction of groundwater flow and a water multiparameter sonde was used to characterize the water chemistry at each point of the springs. As a result, from the six springs studied only three springs are connected which are Yelton, Onyx and Boiling Spring. Regarding to water chemistry this …

Cellular Level Changes Induced In Cancer Cells Due To Microgravity, Sai Deepika Reddy Yaram

Graduate Theses, Dissertations, and Problem Reports (ETD)

The increase in human activity in space has revealed several kinds of physiological challenges brought on by the absence of Earth's gravitational pull. NASA observed that during spaceflight, astronauts experience a 1-1.5% monthly loss in the mineral density of weight-bearing bones, with rehabilitation efforts on Earth often proving ineffective. This issue extends to muscles, the neuro vestibular system, the cardiovascular system, and vision. Cancer has emerged as a significant cause of mortality among astronauts, with studies up to 2017 showing that 41.3% of natural cause deaths among U.S. astronauts were due to cancer, primarily due to space radiation exposure. Cancer …

The Integration Of Neuromorphic Computing In Autonomous Robotic Systems, Md Abu Bakr Siddique

Dissertations, Master's Theses and Master's Reports

Deep Neural Networks (DNNs) have come a long way in many cognitive tasks by training on large, labeled datasets. However, this method has problems in places with limited data and energy, like when planetary robots are used or when edge computing is used [1]. In contrast to this data-heavy approach, animals demonstrate an innate ability to learn by communicating with their environment and forming associative memories among events and entities, a process known as associative learning [2-4]. For instance, rats in a T-maze learn to associate different stimuli with outcomes through exploration without needing labeled data [5]. This learning paradigm …

Evaluation Of Air Emissions For Cured-In-Place Pipe Construction Method For Sewer Pipe Rehabilitation, Rasoul Adnan Abbas

Civil Engineering Dissertations - Archive

The aging underground pipelines in the United States, which include sewer and water supply systems, have exceeded their design lifespan and will have to be renewed or replaced to continue providing these vital services to communities. Two methods can be used to replace the pipes: open-cut and trenchless technologies. Trenchless methods offer several advantages in urban areas: a small work area, lower operational and maintenance costs, and fewer negative impacts on the surrounding communities. Among the trenchless options, the CIPP method has been chosen for this research since it minimizes surface disruption, making it particularly well-suited for densely populated areas …

Continuous, Platform Purification Of Viral Products Using Aqueous Two-Phase Extraction, Natalie M. Nold

Dissertations, Master's Theses and Master's Reports

Cell and gene therapies, a ground-breaking class of drugs that can heal genetic diseases and cure cancer, often have treatment costs over $1 million. Most cell and gene therapies are delivered using virus-based delivery vectors, which are incredibly expensive to manufacture. Liquid chromatography is the favored method of viral vector purification since it is easily scalable and can be functionalized to purify by biospecificity, charge, hydrophobicity, or size. However, chromatography-based purification processes are highly expensive and usually only recover 20-50% of the viral vectors. There is currently no platform purification strategy for viral vectors due to differences in their surface …