Digital Commons Network^™

Scanning Electron Microscopy Of The Osteoclast-Bone Interface In Vivo, H. Zhou, R. Chernecky, J. E. Davies

Cells and Materials

Rat femoral bones were studied by scanning electron microscopy to demonstrate the morphology of osteoclast- bone matrix interfacial relationships. Two general morphological types of actively resorbing osteoclasts were observed . One cell type was approximately ovoid with highly fimbriated borders, fully attached and closely adapted to the resorption surface. The ruffled border of such cells was composed of a number of individual filopodia which were of uniform , regular shape and approximately 2-3 μm in length and 150 nm in diameter. They were found to penetrate the bone matrix to a depth of 1 μm and interdigitated with the surrounding …

Attachment Kinetics And Differentiations Of Osteoblasts On Different Biomaterials, U. Meyer, D. H. Szulczewski, K Moller, H. Heide, D. B. Jones

Cells and Materials

Primary bovine osteoblast cell cultures were used to study the response of osteoblasts ton three different biomaterials, ionomeric cement (IC), tri-calcium phosphate poly-L-lactic acid composite foil (TCP) and poly-L-lactate-polycitric acid composite foil (PLA). The cells which grew on the materials produced all typical bone matrix proteins and were osteoblast-Iike as shown by immuno-staining. Analysis of the cell attachment kinetics revealed significant differences within the first 7 hours between the various materials. The best rate of cell attachment was found on the IC surface, followed by the TCP and then the PLA surface. The kinetics of attach ment appeared to be …

Cell-Degradation Of Calcium Phosphate Ceramics, P. Frayssinet, N. Rouquet, F. Tourenne, J. Fages, D. Hardy, G. Bonel

Cells and Materials

Calcium phosphate ceramics are used in bone surgery under different forms: dense or porous ceramic s as bone substitute, thin ceramic coatings on metallic implants as an osseointegration enhancer. Their degradation depends on their physico-chemical properties and particularly on their chemical composition. Natural calcium phosphates of bone are degraded by mononuclear or multinuclear cells and the extracellular matrix induces the differentiation of the degrading-cells. Hydroxyapatite, which is one of the most used calcium phosphates , is known as a low degradation material. However, the histological analysis of implanted HA-materials both in animals and in humans showed that a cellular degradation …

Analysis Of The Bony Interface With Various Types Of Hydroxyapatite In Vitro, J. D. De Bruijn, J. S. Flach, K. De Groot, C. A. Van Blitterswijk, J. E. Davies

Cells and Materials

Rat bone marrow cells, capable of forming bonelike tissue, were cultured on three types of plasma sprayed hydroxyapatite that differed in degree of crystallinity from 15%, 43% to 69%. The interface between the mineralized extracellular matrix and the hydroxyapatite was studied with scanning and transmission electron microscopy. At the onset of bone formation, calcium and phosphorous-rich afibrillar globules, with a diameter varying from 0.2 to 0.8 μm, were produced and deposited onto the different substrata. These globules subsequently fused to form a homogeneous layer to which collagen fibres became anchored. Individual globules could be distinguished on the non-degrading 69 % …

Intravital Microscopic Evidence That Polylactide-Polyglycolide (Plga) Delays Neo-Osteogenesis And Neo-Angiogenesis In Healing Bone, H. Winet, J. O. Hollinger

Cells and Materials

The bone chamber implant (BCI) has allowed monitoring a healing bone defect as well as the effect of an eroding implant on the healing process. The BCI is a useful tool and intravital microscopy a valuable technique for obtaining quantitative data chronicling osseous wound healing. The physiological parameters that form the initial data base documenting healing are neo-osteogenesis and neo-angiogenesis. This review compares and characterizes osseous wound healing in a BCI loaded with an erodible copolymer, PLGA (polylactide-polyglycolide). To determine if a statistically significant deviation from normal healing had occurred, the results were compared with present and historical controls. In …

Industrial Agricultural Products Center Review 1993

Industrial Agricultural Products Center: Publications and Information

Significant progress has been made toward the goals of the Center. Activities have centered on the identification of opportunities in terms of new industrial uses of agricultural commodities. Assistance has been provided to UNL faculty to procure funding research in the respective areas. In other cases, specific faculty positions have been identified. Subsequent hirings have built a strong group of faculty affiliates.

The main emphasis in the Center has been research on developing new processes and products. A fairly comprehensive list of projects funded and the faculty involved are listed in a subsequent section. Two strategic planning sessions have been …

Phosphorus Accumulation In Bottom Sediments Of Retention/Detention Ponds, Kriss Young Kaye

Retrospective Theses and Dissertations

Laboratory and field studies were conducted to characterize phosphorus in bottom sediments for retention/detention ponds. The laboratory studies, including batch and column experiments, were conducted to assess possible removal processes. Sediment core samples were collected from detention ponds receiving urban runoff. These cores were analyzed for phosphorus at different layers including accumulated top sediments and the lower parent soil beneath it at a depth of 1, 3, 5, 10 and greater than 10 cm. The phosphorus accumulation rate was found to decline with calculated overflow rates from an average storm. Also attenuation of phosphorus with sediment depth followed an exponential …

Relative Humidity And Temperature Effects On Tensile Strength Of Edible Protein And Cellulose Ether Films, A. Gennadios, H. J. Park, Curtis L. Weller

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The effect of relative humidity and temperature on tensile strength of two types of protein-based [corn zein (CZ) and wheat gluten (WG)] and two types of cellulosic [methylcellulose (MC) and hydroxypropyl cellulose (HPC)] hydrophilic edible films was investigated.

Generation And Legal Regulation Of Odors Form Animal Manures, J. L. Taraba, R. M. Williams

Agricultural Engineering Extension Updates

The present trend of animal and dairy product producers is toward total confinement facilities and larger animal herds. Generally the rewards to the producer for these practices are higher economic returns which result from higher feeding efficiencies and better quality products. However, total confinement is not without problems that were previously not present or were minimal in nature when animals were maintained in fields. Confinement facilities have a greater impact on environmental quality of both water and air. Presently, one of the major problems facing animal and dairy product producers is odor production. The greatest number of complaints concerning environmental …

Solid Livestock Waste Regulatory Requirements, J. L. Taraba, W. D. Thom, Anthony J. Pescatore, R. M. Williams

Agricultural Engineering Extension Updates

Proper management and utilization of livestock waste can be a significant contribution to the economic well being of a livestock enterprise, since the wastes have considerable economic value if properly utilized. When returned to the soil as fertilize or soil conditioner, the value as a nutrient source has been will established. More recently, processed livestock wastes have been utilized as a high protein feed additives. Livestock waste should be considered a resource to be utilized rather than a disposal problem.

Rio Mimbres Acequia Systems: An Oral History, Neal W. Ackerly

Books

This volume contains transcripts of interviews with various residents collected during the 1993 field phase of the survey of acequia systems in the Mimbres Valley. As noted in Volume 1, additional interviews were conducted with other residents, but were not recorded. Accordingly, no transcript of these interview sessions cannot be included here.

The individuals interviewed, as well as the abbreviated names appearing in these transcripts, included John VonTress (JVT, age 57), Regis McSherry (RM, age 67), Sam Grijalva (age 73), Charles Disert (age 84), and Horace Bounds (HB, age 90). Vesta McSherry, appearing as VM in transcripts, provided additional information …

The Removal Of Lead-Based Paint From Steel Bridges, Theodore Hopwood Ii, Christopher M. Oberst

Kentucky Transportation Center Research Report

The purpose of this study was to assist the Department of Highways in conducting bridge-painting operations involving removal of existing lead-based paint. Pertinent state and Federal regulations affecting generation of hazardous wastes, limiting environmental pollution and protecting public and worker safety were obtained and reviewed.State highway agendas were surveyed relative to how they conducted maintenance bridge painting involving lead-based. paints and comply with applicable regulations. Information from the painting industry related to sale removal of lead-based paints was also reviewed.

Based upon the reviews of applicable regulations, operations of other state highway agencies and painting industry practices, the best demonstrated …

Traffic Accident Investigation, Kenneth R. Agent, Jerry G. Pigman

Kentucky Transportation Center Research Report

The information given in this report deals with the reporting and at-scene investigation of traffic accidents. The procedure to complete the uniform police traffic accident report used in Kentucky is described. Also, the proper procedure necessary to document the accident scene is described. The objective of this report is to summarize the material included in the "Traffic Accident Investigation" workshop. The workshop also included case studies which were used to illustrate the procedures for proper reporting and documenting traffic accidents. Slides were used to show traffic accident scenes in order to illustrate the proper interpretation and documentation of accident scene …

Kentucky Traffic Accident Facts 1993 Report, Kentucky State Police

Kentucky Transportation Center Research Report

KENTUCKY'S TRAFFIC ACCIDENT FACTS report for 1993 is based on accident reports submitted to the Accident Unit housed in the Kentucky State Police Information Services Branch, Records Section. As required by Kentucky Revised Statutes 189.635, "every law enforcement agency whose officers investigate a vehicle accident of which a report must be made ... shall file a report of the accident ... within ten days after investigation of the accident upon forms supplied by the bureau." The stated purpose of this requirement is to utilize data on traffic accidents" for such purposes as will improve the traffic safety program in the …

Computer Simulation Of Three-Dimensional Mechanical Assemblies: Part I - General Formulation, Yong Fang, Frank W. Liou

Mechanical and Aerospace Engineering Faculty Research & Creative Works

In Part I of this paper, a dynamic modeling system for the simulation of three-dimensional mechanical assemblies is presented. With this simulation tool, a designer can interactively create an assembly of mechanical components ready for dynamic analysis. The modeling system presented in this paper include» the derivation of the equations of motion of spatial multi-body systems, and the formulation of the equations to model the associated collision detection and collision responses. Part II of this paper is to introduce the geometry modeling and computer simulation of 3D systems.

Photovoltaic-Powered Water Pumping-Design, And Implementation: Case Studies In Wyoming, Badrul H. Chowdhury, S. Ula, K. Stokes

Electrical and Computer Engineering Faculty Research & Creative Works

A photovoltaic-powered water pumping project comprising of several rural electric cooperatives and their customers is described in detail. A number of remote water pumping installations in the state of Wyoming, USA, are currently operating as a result of this project. The design, installation and performance monitoring of these systems are discussed. In general, it can be stated that PV-powered water pumping in this state has been a cost-effective alternative to distribution line extension or other conventional means of water pumping. Customer satisfaction, in terms of functional adequacy and low maintenance requirements of these systems, is high. The benefit for the …

Overview Of Acerc Comprehensive Model Development, Thomas H. Fletcher, Scott C. Hill

Faculty Publications

A major objective of the Advanced Combustion Engineering Research Center (ACERC) is the development of comprehensive combustion models to help in the solution of critical national combustion problems. Computer models incorporate research and technology results from center projects and from external research programs. The synergistic integration of scientific knowledge that is expected from the NSF engineering research centers is demonstrated to a great extent at ACERC by the development of these software tools. The transfer of technology from ACERC to industry is also accomplished in part by the implementation of the models at industrial firms. The effort to develop such …

Advanced Graphics Zoom In On Operations, Max Darwin Anderson, Hardy J. Pottinger, C. M. Schroeder

Electrical and Computer Engineering Faculty Research & Creative Works

No abstract provided.

Computer Simulation Of Three-Dimensional Mechanical Assemblies: Part Ii - Computer Simulation, T. C. Chou, Frank W. Liou

Mechanical and Aerospace Engineering Faculty Research & Creative Works

Computer simulation of the kinematic and dynamic behaviors of mechanical assemblies has become a very important tool in design and manufacturing, because the designer can foresee how a product is going to perform before the product is actually fabricated. However, up to now, the most current simulation modules are based on analysis from another kinematic or dynamic module by specifying the mating conditions between components, and then displaying the motion on the screen. This computer simulation actually performs similarly to a movie and can only provide visual checking. The drawback of this simulation approach is that designers are forced to …

Interposed Abdominal Compression Cardiopulmonary Resuscitation: Are We Missing The Mark In Clinical Trials?, Charles F. Babbs

Weldon School of Biomedical Engineering Faculty Publications

Straightforward considerations of abdominal anatomy in human beings set tight constraints on the theoretically optimal technique for abdominal compressions during interposed abdominal compression (IAC)-CPR. The location and extent of the abdominal aorta lead naturally to the recommendation that IAC be applied at a level corresponding to the lower two thirds of the sterno-umbilical line. The force vector required to achieve contact compression of the abdominal aorta is inclined in the transverse plane at an angle of 11 degrees from the vertical toward the left. Such slightly angled compression subjects the abdominal aorta to maximally flattening; while the inferior vena cava …

Interposed Abdominal Compression-Cpr: A Case Study In Cardiac Arrest Research, Charles F. Babbs

Weldon School of Biomedical Engineering Faculty Publications

When the abdomen is compressed manually in counterpoint to the rhythm of chest compression, in the performance of interposed abdominal compression-CPR, artificial circulation is approximately doubled in animal experiments and in electronic models of the circulatory system. These studies suggest that external manual compression of the abdominal aorta acts like an intra-aortic balloon pump to increase aortic pressure, whereas external manual compression of the abdominal veins acts to prime the right heart and pulmonary vessels before the next chest compression. As a result, perfusion pressures and flows are increased. Several clinical studies of this technique have shown promising results, including …

Development Of A Rapidly Computable Descriptor Of Prostate Tissue Temperature During Transurethral Conductive Heat Therapy For Benign Prostate Hyperplasia, U H. Patel, Charles F. Babbs

Weldon School of Biomedical Engineering Faculty Publications

Benign prostate hyperplasia (BPH) is a condition in older men in which the mass of tissue in the prostate gland gradually increases over the course of many years, ultimately leading to urinary outflow obstruction. Current treatment of this condition is to surgically remove the obstructing tissue. One novel alternative therapy being studied is transurethral thermocoagulation of excessive prostatic mass. In this approach, a heat emitting catheter is placed in the prostatic urethra, and the intraprostatic segment of the catheter is heated to temperatures above 60 oC for one hour. Two dimensional cylindrical coordinate computer simulations of this treatment modality were …

Chemical Tracing Techniques For Evaluating Rill Hydraulics, John E. Gilley, Eugene R. Kottwitz

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Development of water erοsiοn and surface water quality control practices requires information concerning the hydraulic characteristics of upland areas. The relatively small flow rates normally found within rills make measurement of hydraulic parameters difficult. Chemical tracing procedures, originally developed for stream and river systems, have been successfully used to measure rill flow properties. A chemical tracer of known concentration is added to the rill and by knowing the degree of dilutiοn at a downstream sampling point, flow rate can be calculated. Rill flow velocity can be measured by determining the time required for a slug of tracer material to travel …

A Prototype Computer Based Tutorial For The Use Of Highway Patrol Radar Guns, William J. Grenney, Thad E. Senti

Reports

Executive Summary: The software contained in this package, termed POSAT (Police Officer Standards and Training) is the prototype knowledge-base decision support system developed specifically for the Utah State Police Academy. All police officers must be certified annual for the use of radar guns. This self-study computer program is intended to reduce the cost of providing field training for hundreds of police officers each year. By installing the POSAT software at remote locations, polic officers within a certain districut need only to travel to the nearest remote testing sight to take the exam and renew their radar certification. POSAT automatically transmits …

Development Of Mountain Climate Generator And Snowpack Model For Erosion Predictions In The Western United States Using Wepp: Phase Iv, David S. Bowles, U. Lall, D. G. Tarboton, E. Malek, B. Rajagopalan, T. Chowdhury, E. Kluzak

Reports

Executive Summary: Introduction: This report summarizes work conducted during the funding period (December 1, 1991 through September 30, 1992) of a Research Joint Venture Agreement between the Intermountain Research Station, Forest Service, U. S. Department of Agriculture and the Utah Water Research Laboratory (UWRL), Utah State University (USU). The purpose of the agreement is to develop a Western Mountain Cilmate Generator (MCLIGEN) similar in function to the existing (non-orographic area) Climate Generator (CLIGEN), which is part of the Water Erosion Prediciton Project (WEPP) procedure. Aso, we are developing a Western U.S. Snowpack Simulation Model for includsion in WEPP. In the …

The Use Of A Factory Simulation To Evaluate A Flexible Control Structure For Integrated Manufacturing, Ling Ling Pan

Engineering Management & Systems Engineering Theses & Dissertations

Once a control structure for an integrated manufacturing system is decided upon, manufacturing activities are limited by that structure. A flexible control structure is presented as an approach for accommodating a variety of manufacturing activities, without being limited to a single control structure. A flexible control structure is one that allows multiple types of control structure in the manufacturing process. For example, both hierarchical and non-hierarchical structures may be used in a flexible structure. The properties of a flexible control structure are discussed from the point of view of graph theory.

Control structures for automated manufacturing are difficult to evaluate …

High-Level Expression Of A Heterologous Protein In The Milk Of Transgenic Swine Using The Cdna Encoding Human Protein C, William H. Velander, John L. Johnson, Raymond L. Page, Christopher G. Russell, Anuradha Subramanian, Tracy D. Wilkins, Francis C. Gwazdauskas, Christoph Pittius, William N. Drohan

William H. Velander Publications

Transgenic pigs were generated that produced human protein C in their milk at up to 1 g/liter. The gene construct was a fusion gene consisting of the cDNA for human protein C inserted into the first exon of the mouse whey acidic protein gene. These results demonstrate that the mouse whey acidic protein gene contains regulatory elements that can direct cDNA expression at high levels in the pig mammary gland. Recombinant human protein C that was produced at about 380 pg/ml per hr in transgenic pig milk possessed anticoagulant activity that was equivalent to that of protein C derived from …

High-Level Expression Of A Heterologous Protein In The Milk Of Transgenic Swine Using The Cdna Encoding Human Protein C, William H. Velander, John L. Johnson, Raymond L. Page, Chritopher G. Russell, Anuradha Subramanian, Tracy D. Wilkins, Francis C. Gwazdauskas, Christoph Pittus, William N. Drohan

Papers in Biotechnology

Transgenic pigs were generated that produced human protein C in their milk at up to 1 g/liter. The gene construct was a fusion gene consisting of the cDNA for human protein C inserted into the first exon of the mouse whey acidic protein gene. These results demonstrate that the mouse whey acidic protein gene contains regulatory elements that can direct cDNA expression at high levels in the pig mammary gland. Recombinant human protein C that was produced at about 380 pg/ml per hr in transgenic pig milk possessed anticoagulant activity that was equivalent to that of protein C derived from …

A Toposcopic Investigation Of Brain Electrical Activity Induced By Motion Sickness, Dwight A. Roblyer

Theses and Dissertations

A toposcope was constructed as a new tool to study signal frequency and phase relationships in electroencephalogram (EEG) records collected while subjects were experiencing motion sickness. This new tool, named TOPOS, is a software-based, multi-featured version of Grey Walter and Harold Shipton's device which they first produced in the late 1940s. The TOPOS graphical display permits the study of instantaneous frequency relationships between the input channels and a reference signal of fixed or varying frequency. TOPOS adds a correlation grid to aid observers in detecting channel-to-reference correlation levels. Users can also vary several display parameters via menus to optimize the …

Retinal Modeling: Segmenting Motion From Spatio-Temporal Inputs Using Neural Networks, David E. Swanson

Theses and Dissertations

We applied two first-order, linear, time-varying, differential equations to the task of segmenting motion from sequences of images. The equations are modified Grossberg formulas for long-term and short-term memory models characterizing the neurotransmitter and cell-activity levels of a synapse and neuron. We described how a two layered, sensory, neural network can be built using the equations to simulate the amacrine neurons of the retina. The model is defined using adaptive input nodes (adaptive model) and is compared to a similar model without these nodes (O and G model). By replicating the basic amacrine neuron model to form both one- and …