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The Relative Influences Of Climate And Competition On Tree Growth Along Montane Ecotones In The Rocky Mountains, Paige E. Copenhaver-Parry, Ellie Cannon

Faculty Publications - Department of Biological & Molecular Science

Distribution shifts of tree species are likely to be highly dependent upon population performance at distribution edges. Understanding the drivers of aspects of performance, such as growth, at distribution edges is thus crucial to accurately predicting responses of tree species to climate change. Here, we use a Bayesian model and sensitivity analysis to partition the effects of climate and crowding, as a metric of competition, on radial growth of three dominant conifer species along montane ecotones in the Rocky Mountains. These ecotones represent upper and lower distribution edges of two species, and span the distribution interior of the third species. …

Effects Of Nutrient Supplementation On Host-Pathogen Dynamics Of The Amphibian Chytrid Fungus: A Community Approach, Julia C. Buck, Jason R. Rohr, Andrew R. Blaustein

Integrative Biology Faculty and Staff Publications

1. Anthropogenic stressors may influence hosts and their pathogens directly or may alter host–pathogen dynamics indirectly through interactions with other species. For example, in aquatic ecosystems, eutrophication may be associated with increased or decreased disease risk. Conversely, pathogens can influence community structure and function and are increasingly recognised as important members of the ecological communities in which they exist.
2. In outdoor mesocosms, we experimentally manipulated nutrients (nitrogen and phosphorus) and the presence of a fungal pathogen, Batrachochytrium dendrobatidis (Bd), and examined the effects on Bd abundance on larval amphibian hosts (Pseudacris regilla: Hylidae), amphibian traits and community dynamics. We …

Limited Trabecular Bone Density Heterogeneity In The Human Skeleton, Habiba Chirchir Phd

Biological Sciences Faculty Research

There is evidence for variation in trabecular bone density and volume within an individual skeleton, albeit in a few anatomical sites, which is partly dependent on mechanical loading. However, little is known regarding the basic variation in trabecular bone density throughout the skeleton in healthy human adults. This is because research on bone density has been confined to a few skeletal elements, which can be readily measured using available imaging technology particularly in clinical settings. This study comprehensively investigates the distribution of trabecular bone density within the human skeleton in nine skeletal sites (femur, proximal and distal tibia, third metatarsal, …

To Jump Or Not To Jump: Mule Deer (Odocoileus Hemionus) And White-Tailed Deer (Odocoileus Virginianus) Crossing Decisions, Emily N. Burkholder

Undergraduate Theses, Professional Papers, and Capstone Artifacts

There have been recent efforts to enhance ungulate movement through modified fencing structures. Ungulates such as mule deer (Odocoileus hemionus) and white-tailed deer (Odocoileus virginianus) typically negotiate fences by either jumping over fencing or going under. Here we examine crossing success and crossing decisions of mule deer and white-tailed deer and determine factors that influence crossing success and the impending decision to jump over or crawl underneath fencing. Using a BACI (Before-After-Control-Impact) design, we deployed remote cameras along fence lines in three study areas; Canadian Forces Base (CFB) Suffield and OneFour research center in Southeastern Alberta, …

The Role Of Ethylene In Systemic Acquired Resistance In Moss, Keiffer Williams

Undergraduate Honors Thesis Collection

The moss species Physcomitrella patens and Amblystegium serpens were used to categorize the effects of the phytohormone ethylene in the plant-pathogen response mechanism systemic acquired resistance (SAR) in non-vascular plants. The results outlined in this thesis found that individual exposure to the ethylene precursor molecules ACC and ethephon both up-regulated ethylene responsive genes in P. patens, demonstrating a positive response to ethylene exposure in the moss. Exposure to ACC and ethephon in A. serpens followed by inoculation with the fungal pathogen Pythium irregulare demonstrated an ambiguous resistance response induced by ethylene exposure in the moss. The growth rate of P. …

Proteomics Study Of Pre-Harvest Sprouting In Wheat, Pramod Khadka

Electronic Theses and Dissertations

Pre-harvest sprouting (PHS) of wheat (Triticum aestivum L.) is a condition characterized by the early germination of spikes during moist environmental conditions. PHS lowers yield, degrades the quality of grain and thus limits the profits of wheat producers groups. During this investigation, proteomics studies of PHS-resistant and PHS-susceptible wheat embryos were conducted at different imbibition time periods via a cutting-edge technology called iTRAQ. Proteomic analysis revealed that 190 differentially expressed proteins might be involved in various cellular functions, such as carbohydrate metabolism, nitrogen metabolism, stress response, redox regulation, ATP synthesis, and protein translation, during this untimely germination of the wheat …

Analysis Of Starch Branching Enzyme 1 (Sbe1) Gene In Maize, Abiskar Gyawali

Electronic Theses and Dissertations

A previous study revealed a quantitative trait locus (QTL) in maize on the short arm of chromosome 5 (5S) for endosperm amylose content. In that original study, both low and high amylose parental lines, H99ae and GEMS-0067 respectively, were homozygous recessive for amylose extender 1 (ae1) and the polymorphism responsible for this QTL was additive (semi-dominant). Located within the QTL interval is starch branching enzyme 1 (sbe1), which makes it a candidate gene. In order to test whether a polymorphism in sbe1 is the source of this QTL, we crossed a plant homozygous for ae1 and sbe1-Mu onto a …

An Evaluation Of Feeding A Blend Of Essential Oils And Cobalt Lactate To Lactating Dairy Cows, Olivia Jayne Kuester

Electronic Theses and Dissertations

The objective of this study was to determine if the inclusion of a blend of essential oils and cobalt lactate (EOC) in the diet of lactating dairy cows could result in improved nutrient utilization and lactational performance in addition to assessing the application of EOC to control feed mycotoxins and spoilage. The trial was conducted in two experimental periods (Phase 1 (P1) and Phase 2 (P2)) on a commercial dairy in southwest Minnesota equipped with two robotic milking units. Phases 1 and 2 were cross over design trials with two pens. Cows were housed in two freestall pens (57 ± …

Correlation Between Sleep And Lifespan In Drosophila Melanogaster, Courtney H. Fiebelman

Masters Theses

”Sleep has previously been associated with lifespan. Monitoring sleep in any given fly over their lifetime facilitates the ability to predict the lifespan of that given fly. Using this estimate, lifespan can potentially correlate with biological age to identify when health parameters have declined.

To confirm that the prediction algorithm could identify short and long-lived flies, glutathione levels in heads and bodies were compared between two groups. The results showed this to be consistent in the bodies of wild-type Canton S male flies, and showed that glutathione was decreased in the predicted biologically older flies. These data show that glutathione …

Convergence Of Detrital Stoichiometry Predicts Thresholds Of Nutrient-Stimulated Breakdown In Streams, David W. P. Manning, Amy D. Rosemond, Vladislav Gulis, Jonathan P. Benstead, John S. Kominoski, John C. Maerz

Biology Faculty Publications

Nutrient enrichment of detritus‐based streams increases detrital resource quality for consumers and stimulates breakdown rates of particulate organic carbon (C). The relative importance of dissolved inorganic nitrogen (N) vs. phosphorus (P) for detrital quality and their effects on microbial‐ vs. detritivore‐mediated detrital breakdown are poorly understood. We tested effects of experimental N and P additions on detrital stoichiometry (C:N, C:P) and total and microbial breakdown (i.e., with and without detritivorous shredders, respectively) of five detritus types (four leaf litter species and wood) with different initial C : nutrient content. We enriched five headwater streams continuously for two years at different …

Induction And Development Of Anther-Derived Gametic Embryos In Cucumber (Cucumis Sativus L.) By Optimizing The Macronutrient And Agar Concentrations In Culture Medium, Mohammad Reza Abdollahi, Samereh Najafi, Hasan Sarikhani, Sayyed Saeed Moosavi

Turkish Journal of Biology

Anther culture response of a cucumber cultivar (Beta Alpha) and three Iranian landraces of cucumber (Basmenj, Isfahani, and Korki) on MS culture media with an altered macronutrient strength (half, full, 1.5-fold, and double-strength) or various concentrations of agar (0, 3.5, 7, and 14 g L-1) was evaluated in two separate experiments. The effect of different concentrations of agar on the development of callus-derived embryos was also assessed. The androgenic potentials of various cucumbers were significantly (P < 0.01) influenced by the different macronutrient strengths and agar concentrations, although anther culture responses could vary according to the cucumber type. A doubled strength of macronutrients in culture medium resulted in the highest percentage of callogenesis (100%) in Isfahani cucumber, while use of full strength of macronutrients produced the highest percentage of embryogenic calli (83.33%) in the mentioned landrace compared to other tested forms. The maximum number of gametic embryos (1.26 and 1.23 embryos/anther) differentiated from Isfahani anther cultures on full- and half-strength MS medium, respectively. In the second experiment, the highest percentages of callogenesis were obtained in MS culture media solidified with 14 g L-1 and 7 g L-1 agar for Basmenj, with 3.5 g L-1 agar or liquid medium for Isfahani, with 7 g L-1 agar for Beta Alpha, and with 14 g L-1 agar for Korki. Culture media solidified with 7 g L-1 agar produced the highest percentage of embryogenic calli compared to other treatments. Anther culture of Korki cucumber on culture medium with 14 g L-1 agar produced the maximum number of embryos per anther compared to other cucumbers. Improved production of mature somatic embryos (torpedo and cotyledonary embryos) was achieved with higher agar concentrations (7 and 14 g L-1) for the cucumber cultivar and all landraces.

Biochar Amendment Of Grassland Soil May Promote Woody Encroachment By Eastern Red Cedar, Ramesh Laungani, Kenneth Elgersma, Maria Juarez, Kristin Mcelligott, Tyler Kuhfahl

Faculty Work

Although carbon (C) additions to soil have been used in restoration to combat invasive species through changes in soil nitrogen (N) availability, carbon amendments to soil derived from plant material can impact soil N availability in a species-specific manner. As such, amendment-driven feedbacks on N may impact invasive species success and woody encroachment. Soil amendments like biochar, which is often added to soil to increase C storage in grassland systems, may unintentionally encourage woody encroachment into these grasslands by changing soil N dynamics. Few studies have examined biochar impacts on non-agricultural species, particularly invasive species. Woody encroachment of Eastern Red …

Anatomical And Ultrastructural Responses Of Brassica Napus After Long-Term Exposure To Excess Zinc, Seyed Mousa Mousavi Kouhi, Mehrdad Lahouti, Ali Ganjeali, Mohammad Hassan Entezari

Turkish Journal of Biology

Heavy metal contamination resulting from anthropogenic activities is one of the major environmental problems in the modern world. Progress toward creating modified plants that are more efficient in phytoremediation requires an understanding of the anatomical and ultrastructural changes involved in heavy metal accumulation. A large supply of zinc (Zn) is toxic for plants and can lead to functional and structural disorders. The present study investigates the anatomical and ultrastructural responses of Brassica napus (rapeseed) to excess Zn after long-term exposure. Results showed that Zn bioaccumulation in the roots and leaves of plants treated with 350 μM Zn2+ was severely increased, …

Influence Of Plant Species, Location, And Seasonality On Rhizosphere Bacterial Communities In Freshwater Wetlands, Hakchul Lee

Electronic Theses and Dissertations

Plant-associated microbes influence plant functions, including development and growth and abiotic stress tolerance. Rhizosphere microbial communities have been shown to vary according to differences in wetland vegetation. Owing to the complexity of factors influencing soil microbial community structure, a greater knowledge of the relationship between rhizosphere microbial communities and different types of plant species will lead to a deeper understanding of plant-microbe interactions in wetlands. Thus, the purpose of my research was to determine the influence of plant species on rhizosphere microbial communities from different freshwater wetlands. The spatial variability, associated with different wetland sites, of rhizosphere microbial communities was …

Biochemical Composition Of Caribbean Gorgonian Corals And Their Symbiosis Under Ambeint And Stressful Conditions, Kartick Prakash Shirur

Electronic Theses and Dissertations

Gorgonian corals are the dominant benthic fauna on Caribbean reefs. Unlike scleractinian corals, gorgonian abundance on these reefs has remained stable or has increased. Therefore, to predict how climate change will affect the reef community, it is essential to understand gorgonian physiology under ambient and stressful situations. Under ambient conditions, eight gorgonian species hosted different clade B Symbiodinium, and in two gorgonian species in which the bacterial microbiomes were investigated, two distinct communities were found. In the eight gorgonian species, skeletal content varied from 75-96%, with those having more tissue also having more Symbiodinium density within their branches. The composition …

Effect Of The Ethylene Inhibitors Silver Nitrate, Silver Sulfate, And Cobalt Chloride On Micropropagation And Biochemical Parameters In The Cherryrootstocks Cab-6p And Gisela 6, Virginia Sarropoulou, Kortessa Dimassi-Theriou, Ioannis Therios

Turkish Journal of Biology

In the present study, the effects of three ethylene inhibitors, silver nitrate (AgNO3), silver sulfate (Ag2SO4), and cobalt chloride (CoCl2), on the morphogenic and biochemical responses in CAB-6P and Gisela 6 cherry rootstocks were investigated. In both rootstocks, AgNO3 and Ag2SO4 promoted shoot regeneration while CoCl2 had such an effect only in Gisela 6. The rooting percentage of CAB-6P and Gisela 6 explants was diminished with AgNO3, Ag2SO4, and CoCl2. AgNO3 increased root number (40 μM) and root length (50 μM) in the CAB-6P rootstock while it had an inhibitory effect in Gisela 6. Ag2SO4 and CoCl2 enhanced root length …

Dynamic And Static Stretching Are Equally Effective Techniques For Female High School Soccer Players, Morgan Stefanoff

The University Honors Program

Female athletes are more likely than males to experience anterior cruciate ligament (ACL) tears. Common mechanisms for ACL injury include anterior tibial shears occurring between 20-30 degrees of knee flexion and knee valgus (adduction). Stretching prior to exercise reduces the likelihood of ACL tears. I tested the hypothesis that dynamic stretching would reduce the likelihood of ACL tears as indexed by a greater range of motion (ROM) and greater joint stability (knee valgus) than static stretching. Twenty-seven healthy female soccer athletes in high school (ages 15-18) participated. The athletes completed a sit-and-reach test for flexibility, squats to assess ROM, and …

Full Issue: Volume 9, Number 2

The Science Journal of the Lander College of Arts and Sciences

No abstract provided.

Seasonal Changes In Biomass Of Two Comfreshwater Wetland Plants: Storage Of Nutrients, Emily Kathryn Mccann

Electronic Theses and Dissertations

In this study, I quantified differences in above- and belowground biomass and storage of nutrients. I hypothesized that storage of phosphorous would be greater in belowground biomass in the non-growing season compared to the growing. I also hypothesized that the storage of phosphorous would be greater in aboveground biomass in the growing season compared to the non-growing season. Furthermore, I hypothesized that the total amount of nutrients in plant tissue would be greater in the growing season, than the non-growing season. Typha latifolia and Carex lurida were placed in mesocosms at the University of Mississippi Field Station (UMFS) and dosed …

Age-Old Palms On Florida’S Ancient Ridges, Warren G. Abrahamson Ii

Faculty Journal Articles

Genetic fingerprinting, ramet growth rate determinations, and mathematical modeling (Minimum branching trees) were used to estimate clone ages of saw palmettos at Archbold Biological Station in Florida. Growth rates indicate that 500 year old saw palmetto ramets are common. Genetically identified clones (genets) are estimated to be thousands of years old, likely exceeding 5,000 years of age.

Flight Mechanics And Control Of Escape Manoeuvres In Hummingbirds. I. Flight Kinematics, Bo Cheng, Bret W. Tobalske, Donald R. Powers, Tyson L. Hedrick, Susan M. Wethington, George T.C. Chiu, Xinyan Deng

Faculty Publications - Department of Biological & Molecular Science

Hummingbirds are nature’s masters of aerobatic manoeuvres. Previous research shows that hummingbirds and insects converged evolutionarily upon similar aerodynamic mechanisms and kinematics in hovering. Herein, we use three-dimensional kinematic data to begin to test for similar convergence of kinematics used for escape flight and to explore the effects of body size upon manoeuvring. We studied four hummingbird species in North America including two large species (magnificent hummingbird, Eugenes fulgens, 7.8 g, and blue-throated hummingbird, Lampornis clemenciae, 8.0 g) and two smaller species (broad-billed hummingbird, Cynanthus latirostris, 3.4 g, and black-chinned hummingbirds Archilochus alexandri, 3.1 g). Starting from a steady hover, …

Automated Solid-Substrate Cultivation Of The Anaerobic Bacterium Clostridium Thermocellum, Mathew J. Ruwaya

Theses and Dissertations--Biosystems and Agricultural Engineering

The organism Clostridium thermocellum grows on cellulosic substrates and produces ethanol, acetate, lactate, formic acid, and CO₂. The organic acids produced alter the growth environment in which the bacteria grows and ultimately inhibit bacterial growth. One method which has been used successfully to maintain the system at acceptable growth conditions is to intermittently flush out the spent media and metabolic products and replace with new fermentation media. Our goal was to design and build an automated system that will automatically flush the spent media from the growing culture and resupply new media without manual intervention. An automated control …

Consequences Of Elevated Salt Concentrations Expression Profiles In The Rhizobium S. Meliloti 1021 Likely Involved In Heat And Desiccation Stress, Jan A.C. Vriezen, Caroline M. Finn

Biological Sciences: Faculty Publications

No abstract provided.

Appearance Of Membrane Compromised, Viable But Not Culturable And Culturable Rhizobial Cells As A Consequence Of Desiccation, Jan A.C. Vriezen, Frans J. De Bruijn

Biological Sciences: Faculty Publications

For agricultural purposes, drought related stresses negatively affect the Rhizobiaceae in at least three ways. Firstly, rhizobial populations are affected by desertification of agricultural soils. Secondly, the quality of dry-base inocula, also called formula, is negatively affected by a drying step, and thirdly, rhizosphere bacteria protect crop-plants against drought. Although survival of cultivatable bacteria has been studied intensively in dry-base seed inocula and in-vitro, thus far research has only marginally addressed the bacterial cell, its cellular structures and physiology. Many questions remain regarding the sensing of and physiological response of rhizobia to desiccation. This review will focus on the three …

Evidence Of Female Sex Pheromones And Characterization Of The Cuticular Lipids Of Unfed, Adult Male Versus Female Blacklegged Ticks, Ixodes Scapularis, Ann L. Carr, Daniel E. Sonenshine, John B. Strider Jr., R. Michael Roe

Biological Sciences Faculty Publications

Copulation in I. scapularis involves physical contact between the male and female (on or off the host), male mounting the female, insertion/maintenance of the male chelicerae in the female genital pore (initiates spermatophore production), and the transfer of the spermatophore by the male into the female genital pore. Bioassays determined that male mounting behavior/chelicerae insertion required direct contact with the female likely requiring non-volatile chemical cues with no evidence of a female volatile sex pheromone to attract males. Unfed virgin adult females and replete mated adult females elicited the highest rates of male chelicerae insertion with part fed virgin adult …

Antagonistic Interactions Between Aquatic Turtles And Territorial Sunfish, Tara Jane Snyder

Theses, Dissertations and Culminating Projects

When sunfish (Lepomis spp.) breed, parents defend nest sites and are highly aggressive and territorial against conspecifics and other fish and invertebrate species. However, little is known of their possible effects on sympatric turtle species. In this study I examined the relationship between the musk turtle (Sternotherus odoratus), painted turtle (Chrysemys picta), pumpkinseed sunfish (.Lepomis gibbosus), and bluegill sunfish (Lepomis macrochirus) within Lake Wapalanne, Sandyston, New Jersey. My hypotheses were: presence of musk and painted turtle individuals would diminish within the sunfish nest habitats during the breeding period and would revert back to normal numbers of captures post-sunfish breeding; musk …

Evaluation Of Genotoxic And Cytologic Effects Of Environmental Stressin Wheat Species With Different Ploidy Levels, Sefa Pekol, Mehmet Cengi̇z Baloğlu, Yasemi̇n Çeli̇k Altunoğlu

Turkish Journal of Biology

Drought and salinity induce biochemical, physiological, morphological, and genetic responses in plants. To understand the damage to different wheat species caused by these stresses, we investigated root-shoot growth, cell division, chromosomal aberrations, and nucleolus organizer regions (AgNORs) in root tip cells. Inhibition of root and shoot growth were observed in diploid, tetraploid, and hexaploid wheat genotypes after salt and drought stresses. Both stresses had adverse effects on dividing cells in root tips of all wheat species and caused a decrease in mitotic index values. Salt and drought lead to the induction of chromosome aberrations and repression of interphase AgNOR parameters. …

Comparative And Phylogenetic Analysis Of Zinc Transporter Genes/Proteins In Plants, Recep Vatansever, İbrahi̇m İlker Özyi̇ği̇t, Ertuğrul Fi̇li̇z

Turkish Journal of Biology

Zinc is an important catalytic element for more than 300 enzymes and plays a structural role in the stabilization of many proteins. Protein domain analysis showed that identified Zn transporter proteins belong to the ZIP protein family (PF02535). Zn transporter sequences were found to have similar molecular weights (33.1-51.4 kDa) and amino acid lengths (306-478 amino acids) with 5.31-8.92 pI values. Subcellular localization of Zn transporters was predicted as the plasma membrane. They had 6-9 putative transmembrane domains with a variable region between TM-3 and TM-4, which could contain a potential histidine-rich metal-binding domain. Moreover, alignment analysis showed that the …

The Impact Of Oxygen On Bacterial Enteric Pathogens, Nathan Wallace, Ashley N. Zani, Elizabeth A. Abrams, Yvonne Sun

Biology Faculty Publications

Bacterial enteric pathogens are responsible for a tremendous amount of foodborne illnesses every year through the consumption of contaminated food products. During their transit from contaminated food sources to the host gastrointestinal tract, these pathogens are exposed and must adapt to fluctuating oxygen levels to successfully colonize the host and cause diseases. However, the majority of enteric infection research has been conducted under aerobic conditions. To raise awareness of the importance in understanding the impact of oxygen, or lack of oxygen, on enteric pathogenesis, we describe in this review the metabolic and physiological responses of nine bacterial enteric pathogens exposed …

The Effects Of Oral Probiotic Supplements On The Human Gut Microbiome, Erin Hudnall

Honors Theses

A diverse bacterial community makes up the human gut microbiome and is essential not only to digestion, but also to other physiological processes. This bacterial community is subject to change due to external factors including, but not limited to, age, diet, and lifestyle. This study observed the effects of oral probiotic supplements on this bacterial community. Fecal samples were collected from a single subject once each week during alternating two- to six-week cycles of taking and abstaining from the probiotic supplement. The samples were purified, the bacterial cells within lysed, and the enclosed DNA was collected. A portion of the …