Digital Commons Network^™

Vegetation Effects On Rhizosphere Microbial Communities In Coastal Wetlands Of South Mississippi, Sean Woo Kang

Honors Theses

The Mississippi vegetated coastal wetlands consist of many salt and brackish marshes. In those marshes, there are two plant species Spartina alterniflora and Juncus roemerianus that thrive in those environments. This would not be possible without the benefits of microbial communities that live in the portion of the plant's soil called the rhizosphere. The rhizosphere is crucial for plant nutrition, health, and quality. It supports the biomass and activity of microorganisms for carbon sequestration, ecosystem functioning, and nutrient cycling in natural ecosystems. To investigate the vegetation effects on rhizosphere microbial communities in coastal wetlands, plant samples and their rhizosphere soils …

Factors Affecting Injury History And Their Overall Impact On Athletic Performance In Distance Runners, Trevor Norris

Williams Honors College, Honors Research Projects

Distance running is a very precise mechanism of many intricate movements and abilities of the body. The amount of miles that a distance runner must run puts a lot of very intense stresses on one’s body. These stresses often appear at the bone level and lead to many injuries such as stress reactions and stress fractures but also may play important roles in tissue damages such as strains and pulls. In this study, we used division one college distance runners and examined many intricacies between their level of fitness and overall composition. These include looking at imbalances, dominant traits, and …

Insights Into Triterpene Metabolism In Model Monocotyledonous And Oilseed Plants Genetically Engineered With Genes From Botryococcus Braunii, Chase F. Kempinski

Theses and Dissertations--Plant and Soil Sciences

Isoprenoids are one of the most diverse classes of natural products and are all derived from universal five carbon, prenyl precursors. Squalene and botryococcene are linear, hydrocarbon triterpenes (thirty carbon compounds with six prenyl units) that have industrial and medicinal values. Squalene is produced by all eukaryotes as it is the first committed precursor to sterols, while botryococcene is uniquely produced by the green algae, Botryococcus braunii (race B). Natural sources for these compounds exist, but there is a desire for more renewable production platforms. The model plant Arabidopsis thaliana was engineered to accumulate botryococcene and squalene in its oil …

Lifelines Winter 2016, Southern Adventist University

Lifelines - Biology Department Newsletter

The Winter 2016 issue of Lifelines contains articles on research on air pollutants, an alumni feature, student mission in Peru, and a pictorial directory of the 2016 biology and allied health graduates.

An Analysis Of The Interaction Between Sin3 And Methionine Metabolism In Drosophila, Mengying Liu

Wayne State University Dissertations

Chromatin modification and cellular metabolism are tightly connected. The mechanism for this cross-talk, however, remains incompletely understood. SIN3 controls histone acetylation through association with the histone deacetylase RPD3. In this study, my major goal is to explore the mechanism of how SIN3 regulates cellular metabolism.

Methionine metabolism generates the major methyl donor S-adenosylmethionine (SAM) for histone methylation. In collaboration with others, I report that reduced levels of some enzymes involved in methionine metabolism and histone demethylases lead to lethality, as well as wing development and cell proliferation defects in Drosophila melanogaster. Additionally, disruption of methionine metabolism can directly affect histone …

Investigation Of The Natural Bacteriocin Nisin And Bioengineered Nisin Derivatives For The Prevention And Control Fo Staphylococcus Biofilms On Medical Device Materials, Leanne Kearney

Theses

Antimicrobial resistance has evolved into being one the major threats facing clinical environments worldwide. It has led to the deerease in effective prevention and treatment of pathogenic bacteria. One such bacterium is Staphylococcus epidermidis. Once considered a relatively harmless and commensal baeterium, it has recently evolved into being one the leading eauses of nosocomial infections. S. epidermidis has proven hamiful due to its ability to fonn detrimental biofilms. Biofilms are structured eonsortia of bacteria embedded in a self-produced polymer matrix that allows adherence to biotic or abiotic surfaces. These biofilms have played a leading role in healthcare-acquired infections with particular …

Diabetes And Physical Activity, Jameela Banu

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Diabetes has been on the rise globally. Although many factors contribute to the increasing risk and manifestation of this disease lack and decreased physical activity/ exercise stands out as one of the major factors. Treatment interventions for prediabetic and diabetic patients include diet and lifestyle changes with enhanced physical activity/ exercise. Several types of physical activity are available for these patients but, recommendations have to be made on an individual basis after giving due consideration to the comorbidities and other risks and barriers. Implementation of progressive resistance therapy may be successful in maintaining glucose homeostasis in diabetic patients.

Design For Mosquito Abundance, Diversity, And Phenology Sampling Within The National Ecological Observatory Network, David Hoekman, Y. P. Springer, C. M. Barker, R. Barrera, M. S. Blackmore, W. E. Bradshaw, D. H. Foley, H. S. Ginsberg, M. H. Hayden, C. M. Holzapfel, Steven A. Juliano, L. D. Kramer, S. L. Ladeau, T. P. Livdahl, C. G. Moore, R. S. Nasci, W. K. Reisen, H. M. Savage

Faculty Publications – Biological Sciences

The National Ecological Observatory Network (NEON) intends to monitor mosquito populations across its broad geographical range of sites because of their prevalence in food webs, sensitivity to abiotic factors, and relevance for human health. We describe the design of mosquito population sampling in the context of NEON's long-term continental scale monitoring program, emphasizing the sampling design schedule, priorities, and collection methods. Freely available NEON data and associated field and laboratory samples, will increase our understanding of how mosquito abundance, demography, diversity, and phenology are responding to land use and climate change.

Chemosensitivity In Mealworms And Darkling Beetles (Tenebrio Molitor) Across Oxygen And Carbon Dioxide Gradients, Andrew King Patterson

Browse all Theses and Dissertations

Breathing in most insects is controlled through a negative feedback loop consisting of signals (O₂, CO₂, pH), sensors (chemoreceptors), integrators (neural ganglia), and effectors (spiracles over tracheae). I hypothesized that mealworms and their adult counterparts Darkling beetles, Tenebrio molitor, can sense anoxic and hyperoxic environments and preferentially avoid these environments. I also hypothesize that mealworms are attracted to hypercarbia while Darkling beetles avoid hypercarbia. I constructed a test arena to create an O₂ or CO₂ gradient. Velocity, total distance traveled, and time spent in each area of the O₂ or CO₂ gradients …

Signal Traits And Oxidative Stress: A Comparative Study Across Populations With Divergent Signals, Maren N. Vitousek, Oldřich Tomášek, Tomáš Albrecht, Matt Wilkins, Rebecca Safran

School of Biological Sciences: Faculty Publications

Diverging populations often shift patterns of signal use – a process that can contribute to reproductive isolation and speciation. Yet it is not clear why most traits gain or lose signal value during divergence. One reason this could occur is because changes in the relationship between signals and relevant physiological parameters degrade the reliability of a signal, or even change its underlying information content. Here we test the hypothesis that the relationship between signal trait elaboration and a central component of organismal health – oxidative stress – differs across closely related populations that have diverged in signal use and preferences. …

Candida Albicans Isw2 Regulates Chlamydospore Suspensor Cell Formation And Virulence In Vivo In A Mouse Model Of Disseminated Candidiasis, Dhammika Navarathna, Ruvini U. Pathirana, Michail S. Lionakis, Kenneth W. Nickerson, David D. Roberts

School of Biological Sciences: Faculty Publications

Formation of chlamydospores by Candida albicans was an established medical diagnostic test to confirm candidiasis before the molecular era. However, the functional role and pathological relevance of this in vitro morphological transition to pathogenesis in vivo remain unclear. We compared the physical properties of in vitro-induced chlamydospores with those of large C. albicans cells purified by density gradient centrifugation from Candida infected mouse kidneys. The morphological and physical properties of these cells in kidneys of mice infected intravenously with wild type C. albicans confirmed that chlamydospores can form in infected kidneys. A previously reported chlamydospore-null Δisw2/ …

Experimental Microbial Evolution Of Extremophiles, Paul H. Blum, Deepak Rudrappa, Raghuveer Singh, Samuel Mccarthy, Benjamin J. Pavlik

School of Biological Sciences: Faculty Publications

Experimental microbial evolutions (EME) involves studying closely a microbial population after it has been through a large number of generations under controlled conditions (Kussell 2013). Adaptive laboratory evolution (ALE) selects for fitness under experimentally imposed conditions (Bennett and Hughes 2009; Dragosits and Mattanovich 2013). However, experimental evolution studies focusing on the contributions of genetic drift and natural mutation rates to evolution are conducted under non-selective conditions to avoid changes imposed by selection (Hindré et al. 2012).

To understand the application of experimental evolutionary methods to extremophiles it is essential to consider the recent growth in this field over the last …

A Stenotrophomonas Maltophilia Strain Evades A Major Caenorhabditis Elegans Defense Pathway, Corin V. White, Brian J. Darby, Robert J. Breeden, Robert J. Breeden, Michael A. Herman

School of Biological Sciences: Faculty Publications

Stenotrophomonas maltophilia is a ubiquitous bacterium and an emerging nosocomial pathogen. This bacterium is resistant to many antibiotics, associated with a number of infections, and a significant health risk, especially for immunocompromised patients. Given that Caenorhabditis elegans shares many conserved genetic pathways and pathway components with higher organisms, the study of its interaction with bacterial pathogens has biomedical implications. S. maltophilia has been isolated in association with nematodes from grassland soils, and it is likely that C. elegans encounters this bacterium in nature. We found that a local S. maltophilia isolate, JCMS, is more virulent than the other S. maltophilia …

The Influence Of Abiotic Factors On The Uptake And Elimination Of 3-Trifluoromethyl-4-Nitrophenol By Larval Sea Lamprey (Petromyzon Marinus), Benjamin Livingston Hlina

Theses and Dissertations (Comprehensive)

The backbone of sea lamprey (Petromyzon marinus) control in the Great Lakes is the use of the piscicide, 3-trifluoromethyl-4-nitrophenol (TFM), which is applied to streams containing larval sea lampreys. However, TFM effectiveness can be influenced by abiotic factors such as pH and temperature, which fluctuate daily and seasonally. The objectives of this thesis were to evaluate the influence of pH, temperature, and season on the toxicity, uptake, and elimination of TFM by larval sea lamprey. Radio-labeled TFM (¹⁴C-TFM) was used to determine how TFM uptake rates varied at different water pHs or temperatures during exposure to …

The Sublethal Physiological Effects Of Exposure To Copper And Silver Mixtures On Rainbow Trout (Oncorhynchus Mykiss), Wesley Truong

Theses and Dissertations (Comprehensive)

The mechanisms behind metal-metal interactions in freshwater environments are currently not well understood. Freshwater environments consist of many different types of metals, from those naturally present such as copper (Cu) and those that originate from anthropogenic sources like silver (Ag). Both Cu and Ag use apical sodium (Na⁺) channels for uptake into the gills of freshwater fish. In the gills, the mechanisms of Cu²⁺ and Ag⁺ toxicity appear to be similar to one another, which is by inhibiting Na⁺ /potassium (K⁺)- adenosine triphosphatase (NKA) and carbonic anhydrase (CA). Inhibition of NKA and CA …

Characterization Of The Interaction Between Basigin And The Pattern Recognition Receptor Tlr4, Josephine Michelle Brown

UNF Graduate Theses and Dissertations

Toll-like receptors (TLRs) are a major group of pattern recognition receptors expressed on the surface of immune cells that recognize molecular patterns associated with all classes of pathogenic microorganisms. TLR4 recognizes the lipopolysaccharide component of Gram-negative bacterial cell walls and is the only TLR known to induce signaling through both the MyD88 and TRIF pathways. Basigin, a ubiquitous cell adhesion molecule, is a member of the immunoglobulin superfamily that has the ability to influence cell signaling mediated by the MyD88 and TRIF pathways, the same signaling pathways induced by the TLR4 receptor protein. Analysis of the Basigin protein sequence indicates …

Flying Under The Lidar: Relating Forest Structure To Bat Community Diversity, Anna Schneider Swanson

Electronic Theses and Dissertations

Bats are important to many ecological processes such as pollination, insect (and by proxy, disease) control, and seed dispersal and can be used to monitor ecosystem health. However, they are facing unprecedented extinction risks from habitat degradation as well as pressures from pathogens (e.g., white-nose syndrome) and wind turbines. LiDAR allows ecologists to measure structural variables of forested landscapes with increased precision and accuracy at broader spatial scales than previously possible. This study used airborne LiDAR to classify forest habitat/canopy structure at the Ordway-Swisher Biological Station (OSBS) in north central Florida. LiDAR data were acquired by the National Ecological Observatory …

Cultivating Trust In Gmo Science: Moving Past The Deficit Model To Productive And Participatory Science Education, Kate Crowley , '16

Senior Theses, Projects, and Awards

Most scientists believe GMOs are safe for human consumption, but a majority of the American public remains unconvinced. I argue that this lack of trust stems from scientists' outdated approach to science communication, namely the deficit model. Instead of seeing the public as empty vessels waiting to be filled with scientific knowledge, the scientific community must recognize that many strongly held beliefs about science issues are socially situated. Only through genuine attempts at participatory, two-way conversations with the public can the scientific community come to understand how public values influence GMO acceptance. While these actions will necessitate a redefining of …

Isolation And Identification Of Bacterial Endosymbionts In The Brooding Brittle Star Amphipholis Squamata, Abbey Rose Tedford

Honors Theses and Capstones

Symbiotic associations with subcuticular bacteria (SCB) have been identified and studied in numerous echinoderms, including the SCB of the brooding brittle star, Amphipholis squamata. These SCB, however, have not been studied using current next generation sequencing technologies. Previous studies on the SCB of A. squamata placed these bacteria in the genus Vibrio (γ-Proteobacteria), but subsequent studies suggested that the SCB are primarily composed of α-Proteobacteria. The present study examines the taxonomic composition of SCB associated with A. squamata from the Northwest Atlantic. DNA was extracted using a CTAB protocol and 16S rRNA sequences were amplified …

Views Of Fathers In Ireland On The Experience And Challenges Of Having A Breast-Feeding Partner, Annemarie Bennett, Daniel Mccartney, John Kearney

Articles

Objective this study investigated the relationship between fathers and breast feeding in Ireland.

Design and method a cross-sectional semi-quantitative questionnaire with closed-ended and open-ended questions was posted to 1398 men with an Irish partner who had given birth 4–7 months previously. Fathers who specified that their partner breast fed their last or only child were questioned about their: influence on the breast feeding decision; ability to assist with breast feeding challenges; preferred type of information on breast feeding; perceived advantages and disadvantages to breast feeding; and views on breastfeeding in public. Data from closed-ended questions on breast feeding were presented …

The Nutritional Value Of Fecal Sacs: Testing The Parental Nutrition Hypothesis, Amanda W. Burns

College of Graduate Studies: Theses & Dissertations

Nest sanitation is an important aspect of parental investment in birds with altricial nestlings. Passerine nestlings produce fecal matter that is encased in a mucous membrane for easy disposal. These fecal sacs are either removed from the nest and transported a distance away or they are ingested by the adult. A decline in fecal sac ingestion rates as nestlings age has been observed in several species. The parental nutrition hypothesis posits that this pattern can be attributed to increasing digestive efficiency as nestlings age. The parental nutrition hypothesis makes three predictions: fecal sac consumption rates should decrease as nestlings age, …

Combined Effects Of Tetrakis Hydroxymethyl Phosphonium Chloride And Ammonium On Plankton Community Structure In Blackwater Pond Mesocosms, Marina N. Osier

College of Graduate Studies: Theses & Dissertations

Flame retardant chemicals enter aquatic systems through municipal and industrial wastewater, and despite being detected in surface waters, most have not been thoroughly assessed for environmental risk. Halogenated flame retardants, known to be persistent and highly toxic to aquatic organisms are being phased out, and replaced with new or under-tested organophosphorus flame retardants. Organophosphorus flame retardants, such as tetrakis hydroxymethyl phosphonium chloride (THPC), often have similar applications to and are generally not as persistent as halogenated flame retardants. Single-species toxicity tests of organophosphorus flame retardants reveal a wide range of toxicity values within the range of environmentally relevant concentrations in …

Spanish River Carbonatite: Its Benefits And Potential Use As A Soil Supplement In Agriculture, James J

Theses and Dissertations (Comprehensive)

A critical problem facing agriculture today is being able to consistently and sustainably provide plants with adequate nutrients for growth. With this problem being exacerbated by the ever-increasing human population, new perspectives and techniques are required to ensure global food security. In order to fully realize potential solutions, however, plant growth and nutrition cannot be exclusively focused upon. The soil-microorganism-plant system is comprised of many interconnected and interdependent processes that together support plant growth: it is upon these processes that the focus must be placed. In this work, the agromineral Spanish River Carbonatite (SRC) is characterized using the framework of …

Implications Of Galling Herbivory On Ground Thaw In Canada’S Northern Boreal Forest, Allison Mcmanus

Theses and Dissertations (Comprehensive)

Canada’s northern boreal forest has faced significant climate warming over the past century. This change can have direct and indirect effects on forest dynamics, including altering the disturbance patterns of herbivores and changing the abundance of natural enemies. Warmer air temperatures can impact the distribution and survival of arthropods, both novel species and resident natural enemies. Gall-inducing mites are a resident natural enemy in high latitude forests and have been shown to drive dramatic reductions in gas-exchange rates in infested plants. Physiological reductions in plants can directly affect the shrub energy balance and underlying soil processes. This can have major …

Nickel And Copper Mixture Toxicity To Daphnia In Soft Water, Prachi Deshpande

Theses and Dissertations (Comprehensive)

Industrially important metals, such as Cu and Ni, sometimes are present at elevated concentrations in lakes, including those in the Sudbury, ON region. Although they are essential metals, their divalent-cation state (Cu²⁺ and Ni²⁺) can be toxic at high concentrations in the water. The free-ion toxicity of each of these metals has been studied in isolation, but rarely as a mixture. The economic importance of Cu²⁺ and Ni²⁺ makes them essential to study in the context of mixture toxicity. The objectives were to: (1) determine Cu and Ni mixture toxicity to Daphnia through acute LC50 …

Three Simple Experiments To Examine The Effect Of Sediment Pollution On Algae-Based Food Webs In Streams, Patrick M. Edwards, Rodney Shroufe

Environmental Science and Management Faculty Publications and Presentations

Streams and stream macroinvertebrates are ideal natural systems for ecological inquiry. We present three simple experiments that students can use to conduct field-based investigations which illustrate the importance of algae-based food webs in streams and measure the effects of sediment pollution (scour and deposition) on stream ecological processes. Over the past 5 years, we have conducted these experiments 19 times with our students. We report on the results and reliability of these experiments and make suggestions for other educators who may want to conduct them.

Status And Future Management Of Grey Goral (Naemorhedus Goral Bedfordi) In Pakistan, Fakhar -I- Abbas, Afsar Mian, Tanveer Akhtar, Thomas P. Rooney

Journal of Bioresource Management

Himalayan grey goral (HGG: Naemorhedus goral bedfordi) is endemic to Himalyas and regarded as Endangered/ Threatened. Study was designed to collect information on population biology, habitat, food and behaviour of population of HGG distributed in Pakistan, trying to understand its present status and conservational potentials. Our data suggest that the population, habitat and the species has sufficient potentials for its survival in the area, if protection from human predation is afforded to the species. HGG population is isolated into 7-8 subpopulations and is facing male-biased mortality, therefore is likely to face bottleneck effects and subsequent population crash ascribed to …

Dna Protective Activity Of Peels Of Some Vegetables Wastes, Aisha Ishaq, Kainaat William

Journal of Bioresource Management

The present study was conducted by using kitchen wastes like peels of different vegetables e.g. cucumber, potato, carrot, chickpea, cauliflower and pea, to evaluate the phytochemical constituents, DNA protection ability and free radicals scavenging activity. Phytochemicals tests showed the presence of carbohydrates, amino acids, glycosides, alkaloids, tannins and steroids in all samples while phlobatannins, anthraquonines and saponins were absent. The DNA protection of samples was checked by DNA protection assay using plasmid pBR322. Cauliflower and cucumber showed best protection at each dilution. DPPH free radicals scavenging assay was used to evaluate the antioxidant activity of peel extract of vegetables. Carrot …

Ethylene, A Hormone At The Center-Stage Of Nodulation, Frédérique C. Guinel

Biology Faculty Publications

Nodulation is the result of a beneficial interaction between legumes and rhizobia. It is a sophisticated process leading to nutrient exchange between the two types of symbionts. In this association, within a nodule, the rhizobia, using energy provided as photosynthates, fix atmospheric nitrogen and convert it to ammonium which is available to the plant. Nodulation is recognized as an essential process in nitrogen cycling and legume crops are known to enrich agricultural soils in nitrogenous compounds. Furthermore, as they are rich in nitrogen, legumes are considered important as staple foods for humans and fodder for animals. To tightly control this …

Characterizing The Role Of Sialylated Milk Glycans And The Infant Gut Microbiota In Growth And Metabolism, Mark Richard Charbonneau

Arts & Sciences Graduate Student Theses and Dissertations

Undernutrition is a pressing and pervasive global health problem. The pathogenesis of this disease remains unclear, but epidemiologic studies indicate that it is not due to food insecurity alone. Moreover, current therapeutic interventions have limited efficacy in preventing or ameliorating the long-term sequelae of undernutrition, including stunting and cognitive deficits. Recent culture-independent studies have demonstrated that the normal postnatal pattern of gut microbiota development is disrupted in children with undernutrition, leading to the proposal that perturbations in gut microbiota development impairs healthy growth of the host. Human breast milk contains a diverse repertoire of free and conjugated human milk oligosaccharides …