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Enhancement Of Direct Shoot Regeneration And Determination Of Bioactivesecondary Metabolites In Leaves Of Galega Officinalis L., Fatma Pehli̇van Karakaş, Günce Ci̇ngöz, Arzu Türker Jan 2016

Enhancement Of Direct Shoot Regeneration And Determination Of Bioactivesecondary Metabolites In Leaves Of Galega Officinalis L., Fatma Pehli̇van Karakaş, Günce Ci̇ngöz, Arzu Türker

Turkish Journal of Biology

Galega officinalis L. is a medicinal plant being used as a galactagogue and an antidiabetic in folk medicine. A new effective micropropagation protocol was established from leaf, stem, node, and root explants of G. officinalis for in vitro plant regeneration and callus induction systems. Murashige and Skoog (MS) medium supplemented with 55 various concentrations and 4 different combinations [benzyl adenine (BA)/naphthalene acetic acid (NAA), BA/indole-3-acetic acid (IAA), thidiazuron (TDZ)/indole-3-butyric acid (IBA), and TDZ/IAA] of plant growth regulators was used for the regeneration systems. The regenerated shoots were observed on only nodal explants through direct organogenesis at the 35th day. The …

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The Effects Of Nutrient And Macronutrient Stress On Certain Secondary Metabolite Accumulations And Redox Regulation In Callus Cultures Of Bellis Perennis L., Günce Ci̇ngöz, Fatma Pehli̇van Karakaş Jan 2016

The Effects Of Nutrient And Macronutrient Stress On Certain Secondary Metabolite Accumulations And Redox Regulation In Callus Cultures Of Bellis Perennis L., Günce Ci̇ngöz, Fatma Pehli̇van Karakaş

Turkish Journal of Biology

The main purpose of this study was to investigate the effects of nutrient and macronutrient stress on complex variations in the phenolic profile and antioxidant status of Bellis perennis L. callus cultures. To obtain stress conditions, callus cultures were grown under insufficient nutrient conditions (different strengths of MS medium) or under conditions of limiting calcium (-Ca), magnesium (-Mg), or both (-Ca & -Mg). Phenolic content of callus cultures obtained from different stress treatments was detected quantitatively by LC-ESI-MS/MS analysis. The major compound was chlorogenic acid in all treatments. The accumulation of hesperidin, rutin hydrate, and chlorogenic acid was significantly induced …

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Carbohydrate Deprivation Upsurges The Expression Of Genes Responsible For Programmed Cell Death In Inflorescence Tissues Of Oil Palm (Elaeis Guineensis Jacq.), Walter Ajambang, Hugo Volkaert, Sudarsono Sudarsono Jan 2016

Carbohydrate Deprivation Upsurges The Expression Of Genes Responsible For Programmed Cell Death In Inflorescence Tissues Of Oil Palm (Elaeis Guineensis Jacq.), Walter Ajambang, Hugo Volkaert, Sudarsono Sudarsono

Turkish Journal of Biology

The aim of this present study was to analyze global gene expression from a developing inflorescence of oil palm under complete defoliation stress. We used the Illumina platform to sequence six cDNA libraries of defoliated and nondefoliated tissues. The frequency of differentially expressed genes (DEGs) decreased with increasing fold change at P ≤ 0.05. Gene ontology analysis revealed that about 15% of all DEGs were responsible for stress response while 13.9% of genes were responsible for cell organization and developmental processes. DAVID functional analysis clustering of DEGs identified a higher enrichment score (ES) for cellular response to stress (ES = …

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Pepper (Capsicum Annuum L.) Anther Culture: Fundamentalresearch And Practical Applications, Teodora Popova Irikova, Spyridon Kintzios, Stanislava Grozeva, Velichka Rodeva Jan 2016

Pepper (Capsicum Annuum L.) Anther Culture: Fundamentalresearch And Practical Applications, Teodora Popova Irikova, Spyridon Kintzios, Stanislava Grozeva, Velichka Rodeva

Turkish Journal of Biology

To create new varieties resistant to biotic and abiotic stress factors that possess high productivity and improved fruit quality, breeders require modern breeding methods and techniques. Double haploid (DH) lines are an invaluable breeding element due to their guaranteed, completely homozygous nature. One of the fastest methods for obtaining DH lines is anther culture. Microspore embryogenesis provides considerable opportunities in the areas of breeding and biotechnology and represents a model system for fundamental biological research. The effectiveness of pepper anther culture is still low, which affects the application of DH lines in breeding programs. A detailed study of the anther …

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Inducing Osmotic Stress Leads To Better Genetic Transformation Efficiency In Cotton (Gossypium Hirsutum L.), Surendra Barpete, Allah Bakhsh, Emi̇ne Anayol, Sancar Fati̇h Özcan, Muhammet Cağri Oğuz, Ömer Cem Karakoç, Sebahatti̇n Özcan Jan 2016

Inducing Osmotic Stress Leads To Better Genetic Transformation Efficiency In Cotton (Gossypium Hirsutum L.), Surendra Barpete, Allah Bakhsh, Emi̇ne Anayol, Sancar Fati̇h Özcan, Muhammet Cağri Oğuz, Ömer Cem Karakoç, Sebahatti̇n Özcan

Turkish Journal of Biology

The present study investigated the effect of different salts on cotton shoot regeneration and transformation efficiency. Two-day-old germinating embryos of a local cotton cultivar (SG-125) were pretreated with 50 mM each of NaCl, CaCl2, and KCl for 60 min. The embryo explants were transformed by cocultivation with Agrobacterium tumefaciens strain LBA 4404 harboring a binary plasmid pTF101.1 that carried the insecticidal gene (cry1Ac) under control of wound-inducible promoter (AoPR1) and bilanafos acetyl reductase (bar) gene for plant selection. The salt-pretreated embryos showed maximum response on regeneration MS medium containing 0.50 mg/L 6-benzylaminopurine (BAP) and 0.10 mg/L indole-3-butyric acid (IBA), also …

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Advancement In Protocol For In Vitro Seed Germination, Regeneration, Bulblet Maturation, And Acclimatization Of Fritillaria Persica, Derya Çakmak, Cuma Karaoğlu, Muhammad Aasim, Cengi̇z Sancak, Sebahatti̇n Özcan Jan 2016

Advancement In Protocol For In Vitro Seed Germination, Regeneration, Bulblet Maturation, And Acclimatization Of Fritillaria Persica, Derya Çakmak, Cuma Karaoğlu, Muhammad Aasim, Cengi̇z Sancak, Sebahatti̇n Özcan

Turkish Journal of Biology

The present study addressed the successful in vitro seed germination, bulblet regeneration, increased bulblet size, hardening, and acclimatization of Persian lily (Fritillaria persica). Seed germination rate was recorded as 86.7% and 96.7% after 2 and 3 months of cold treatment at 4 °C, respectively. Bulblet explants taken from a germination experiment were cultured on Murashige and Skoog (MS) medium supplemented with 0.5-2.0 mg/L thidiazuron (TDZ). Maximum bulblet regeneration frequency and bulblets per explants were achieved on a medium containing 2.0 mg/L TDZ. Leaf disc and leaf scale explants isolated from germinated seedlings were also cultured on MS medium containing 2-6 …

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Sodium Borate Treatment Induces Metabolic Reprogramming In Hepatocellular Carcinoma Through Sirt3 Activation, Berna Üstüner, Hüseyi̇n Çi̇men Jan 2016

Sodium Borate Treatment Induces Metabolic Reprogramming In Hepatocellular Carcinoma Through Sirt3 Activation, Berna Üstüner, Hüseyi̇n Çi̇men

Turkish Journal of Biology

Sirtuins are NAD+-dependent deacetylases and ADP ribosyltransferases that are activated under stress conditions such as calorie restriction and starvation. Boron, which is mostly found in the form of boric acid (BA) or sodium borate (NaB), is known to bind NAD+. In this study, the effect of NaB on hepatoma cell line HEP3B was investigated by analyzing the proteins harvested from NaB-treated and serum-starved HEP3B cells. The NaB treatment (15 μg/mL) led to a decrease in the overall proteome acetylation and particularly in mitochondria, the synthesis rate of respiratory complexes, the amount of cellular reactive oxygen species, and the proliferation rate …

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Prokaryotic Expression And Purification Of Porcine Sox6, Wanxue Wen, Xiaoling Chen, Zhiqing Huang, Meng Xu, Daiwen Chen, Bing Yu, Jun He, Junqiu Luo, Xiangbing Mao, Jie Yu, Ping Zheng, Hong Chen Jan 2016

Prokaryotic Expression And Purification Of Porcine Sox6, Wanxue Wen, Xiaoling Chen, Zhiqing Huang, Meng Xu, Daiwen Chen, Bing Yu, Jun He, Junqiu Luo, Xiangbing Mao, Jie Yu, Ping Zheng, Hong Chen

Turkish Journal of Biology

Sox6, a member of the Sox (Sry-related HMG box) family of transcription factors, plays an important role in embryonic muscle development and adult fast myofiber maintenance. Here we report the expression and purification of a His-tagged version of recombinant porcine Sox6 (pSox6) in Escherichia coli. The recombinant pSox6 was expressed as a C-terminal fusion protein with His6 tag in the E. Coli Rosetta (DE3) host strain. The protein was purified by Ni affinity chromatography, yielding approximately 5 μg/mL. The protein was identified by western blot analysis and confirmed by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) mass spectrometer analysis. In vitro biological …

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Triterpenoid Saponin-Rich Fraction Of Centella Asiatica Decreases Il-1ss Andnf-?B, And Augments Tissue Regeneration And Excision Wound Repair, Amena Mahmood, Ambrish Tiwari, Kazi̇m Şahi̇n, Ömer Küçük, Shakir Ali Jan 2016

Triterpenoid Saponin-Rich Fraction Of Centella Asiatica Decreases Il-1ss Andnf-?B, And Augments Tissue Regeneration And Excision Wound Repair, Amena Mahmood, Ambrish Tiwari, Kazi̇m Şahi̇n, Ömer Küçük, Shakir Ali

Turkish Journal of Biology

Wound healing is an orderly process driven by numerous cellular mediators that aim to accelerate it. In skin conditions such as leprosy and psoriasis, Centella asiatica, which contains high amounts of triterpenoid saponins (TSs), has been reported to have beneficial effects. This study examined the effects of TS fraction of the herb on tissue regeneration and excision wound repair in rat skin. Briefly, TS fraction was prepared from C. Asiatica and applied on the wound area for 7 consecutive days. The IL-1β, NF-κB, and other biochemical parameters were then estimated, followed by examination of inflammatory cell infiltration, epithelialization, angiogenesis, and …

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The Restorative Effect Of Ascorbic Acid On Liver Injury Inducedby Asymmetric Dimethylarginine, Ali̇ Okuyucu, Osman Şaliş, Ömer Alici, Abdullah Güvenli̇, Yüksel Terzi̇, Muhammed Emi̇n Keleş, Fati̇h İlkaya, İbrahi̇m Gören, Hasan Alaçam Jan 2016

The Restorative Effect Of Ascorbic Acid On Liver Injury Inducedby Asymmetric Dimethylarginine, Ali̇ Okuyucu, Osman Şaliş, Ömer Alici, Abdullah Güvenli̇, Yüksel Terzi̇, Muhammed Emi̇n Keleş, Fati̇h İlkaya, İbrahi̇m Gören, Hasan Alaçam

Turkish Journal of Biology

We aimed to determine whether increased levels of asymmetric dimethylarginine (ADMA) directly cause hepatic deterioration and whether it is decreased by ascorbic acid (AA) as an antioxidant. The study included three groups: Control (n = 10), ADMA (n = 10), and ADMA + AA (n = 10). ADMA was administered at 2 mg kg-1 day-1 and AA was given at 50 mg kg-1 day-1 for 10 days. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase, and alkaline phosphatase activities were analyzed. Histomorphological evaluation of the liver was performed based on the Ishak scoring system. When compared with the control, …

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Prediction Of Glycation Sites: New Insights From Protein Structural Analysis, Homero Saenz Suarez, Raul A Poutou Piñales, Janneth Gonzalez Santos, George E. Barreto, Lynda Patricia Prieto Navarrera, Jose A . Saenz Moreno, Patricia Landazuri, Luis A Barrera Avellaneda Jan 2016

Prediction Of Glycation Sites: New Insights From Protein Structural Analysis, Homero Saenz Suarez, Raul A Poutou Piñales, Janneth Gonzalez Santos, George E. Barreto, Lynda Patricia Prieto Navarrera, Jose A . Saenz Moreno, Patricia Landazuri, Luis A Barrera Avellaneda

Turkish Journal of Biology

Glycation of proteins is a nonenzymatic process in which proteins react with reducing sugar molecules. This process takes place at ?-amino (?-NH+) groups of lysine or hydroxylysine residues as well as α-amino groups of N-terminal residues. In the present study glycation of ?-NH+ groups of lysines was computationally analyzed for 26 proteins based on their 3D structures. We determined the spatial relationship with acidic or basic residues and correlated them with the glycation prediction algorithm Netglycate 1.0 software, which employed primary structure exclusively for glycation site prediction. Of the lysines from 19 of the 20 proteins employed to build the …

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Molecular Cloning Of Lipocalin-2 Into A Eukaryotic Vector And Its Expression Inbovine Mammary Epithelial Cells As A Potential Treatment For Bovine Mastitis, Neelesh Sharma, Simrinder Singh Sodhi, Jeong Hyun Kim, Mrinmoy Ghosh, Jiao Jiao Zhang, Deog Bon Koo, Man Jong Kang, Dong Kee Jeong Jan 2016

Molecular Cloning Of Lipocalin-2 Into A Eukaryotic Vector And Its Expression Inbovine Mammary Epithelial Cells As A Potential Treatment For Bovine Mastitis, Neelesh Sharma, Simrinder Singh Sodhi, Jeong Hyun Kim, Mrinmoy Ghosh, Jiao Jiao Zhang, Deog Bon Koo, Man Jong Kang, Dong Kee Jeong

Turkish Journal of Biology

Lipocalin-2 (LCN2) is a 25-kDa protein in the lipocalin family that shows antibacterial activity. The aim of this study was to clone human LCN2 into the mammalian-specific pIRES2-AcGFP1 vector and to determine the antibacterial activity of the recombinant pIRES2-AcGFP1-hLCN2 plasmid against major bacterial pathogens that cause bovine mastitis. The amplified hLCN2 was successfully cloned into the vector, and the plasmid was transfected into bovine mammary epithelial stem cells using nucleofection. Immunochemistry, reverse transcription polymerase chain reaction, and ELISA were used to monitor and quantify hLCN2 expression in the cells. After 1 day of incubation, the concentration of hLCN2 secreted by …

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Analysis Of Protein Expression Changes In The Blood Plasma Of Cows During The Last Month Before Parturition And 2 Months After Calving, Anna Katarzyna Kurpinska, Agnieszka Jarosz, Malgorzata Ozgo, Wieslaw Franciszek Skrzypczak Jan 2016

Analysis Of Protein Expression Changes In The Blood Plasma Of Cows During The Last Month Before Parturition And 2 Months After Calving, Anna Katarzyna Kurpinska, Agnieszka Jarosz, Malgorzata Ozgo, Wieslaw Franciszek Skrzypczak

Turkish Journal of Biology

The organism undergoes tremendous changes at the end of pregnancy and beginning of lactation. The aim of the study was to analyze the physiological changes in cows during the last month of a first pregnancy and the first 2 months of lactation, based on the protein expression in peripheral blood plasma. Changes were observed in the expression of proteins involved in lipid metabolism (apolipoprotein A-I, apolipoprotein E, apolipoprotein J, and apolipoprotein A-IV); immune defense (complement C4 protein and mannose-binding protein C); blood clotting (fibrinogen alpha chain, fibrinogen gamma B-chain, and fibrinogen-like protein 1); as well as reconstruction, regeneration, and defense …

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Cloning And Functional Characterization Of Porcine Sox6, Wanxue Wen, Xiaoling Chen, Daiwen Chen, Bing Yu, Junqiu Luo, Zhiqing Huang Jan 2016

Cloning And Functional Characterization Of Porcine Sox6, Wanxue Wen, Xiaoling Chen, Daiwen Chen, Bing Yu, Junqiu Luo, Zhiqing Huang

Turkish Journal of Biology

Sox6, a fast myofiber-enriched repressor of slow muscle gene expression, plays an important role in the control of skeletal muscle fiber-type transition. Mammalian Sox6 has been isolated and characterized from mice, rats, and humans. In this study, the porcine Sox6 (pSox6) cDNA was cloned by degenerate PCR. The entire open reading frame (ORF) of pSox6 is 2406 bp. The predicted protein is composed of 801 amino acids and contains the HMG box, leucine-zipper motif, and glutamine-rich Q-box. The amino acid similarities between the Sox6 from pig and other mammals range from 90% to 95%. Real-time quantitative PCR analysis indicated that …

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Master Of Science In Biological Sciences Program Overview, Nova Southeastern University Jan 2016

Master Of Science In Biological Sciences Program Overview, Nova Southeastern University

Halmos College of Natural Sciences and Oceanography Course Catalogs

No abstract provided.

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The Identification And Characterization Of Bacterial Endosymbionts In The Planthopper Family, Caliscelidae (Sternorrhyncha: Fulgoroidea, Ghazal Abu-Salim, Kathryn Weglarz Jan 2016

The Identification And Characterization Of Bacterial Endosymbionts In The Planthopper Family, Caliscelidae (Sternorrhyncha: Fulgoroidea, Ghazal Abu-Salim, Kathryn Weglarz

Biology Posters

Plant-sap sucking insects with deficient nutritional diets contain symbiotic microorganisms in a specific organ in the abdomen (bacteriome). Such symbionts were discovered to belong to various bacterial lineages. Ongoing genomic research concluded that these symbiotic organisms provide the essential nutrients that are absent in the insect’s diets such as amino acids and vitamins. While the identities and functions of several sap-sucking lineages have been studied, some lineages such as Fulgoroidea are not understood because recent results failed to confirm the presence of the symbiotic microbes even though older microscopy studies identified microbes in the bacteriomes. The objective of this research …

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Determining Protein-Protein Interactions Of Als-Associated Sod1, Leah A. Shurte Jan 2016

Determining Protein-Protein Interactions Of Als-Associated Sod1, Leah A. Shurte

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Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disorder that occurs due to the death of motor neurons and leads to paralysis and death within three to five years after symptoms present (Byrne et al., 2013). Superoxide Dismutase 1 (SOD1) was first identified to be associated with ALS in 1993. The objective of this study is to determine which proteins interact with wild type and mutant SOD1 and find any similarities or differences between them. ALS is attributed to a gain of toxicity, therefore abnormal protein interactions in mutant SOD1 are important. The results of this study will provide insight on …

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Wolbachia And Sex, Ian J. Aper, Katie Weglarz, Carol Von Dohlen Jan 2016

Wolbachia And Sex, Ian J. Aper, Katie Weglarz, Carol Von Dohlen

Biology Posters

Insects with complex life cycles provide an ideal system for investigating the relationship between sex-skewing bacterial symbionts and the secondary loss of sexual reproduction. This research utilized the insect family Adelgidae, a group notorious for having peculiar and complex life cycles. In this family, some species have lost their sexual generation entirely, trapping them in asexual reproduction. Additionally, these insects are known to harbor multiple symbionts, both obligate and facultative. The array of facultative symbionts that have been detected includes the infamous sex-skewing symbiont Wolbachia. This symbiont is known to induce male killing, cytoplasmic incompatibility, and parthenogenesis in insects. Additionally, …

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The Identification And Characterization Of Bacterial Endosymbionts In The Planthopper Family, Issidae (Sternorrhyncha:Fulgoroidea), Lina Ghabayen Jan 2016

The Identification And Characterization Of Bacterial Endosymbionts In The Planthopper Family, Issidae (Sternorrhyncha:Fulgoroidea), Lina Ghabayen

Biology Posters

Many insects live on nutrient-poor diets; to compensate, they have evolved obligate associations with microorganisms that are transmitted directly between generations. The bacterial associates of some Hemiptera insects have been identified and understood, but several remain unknown. Our objective is to identify the diversity of bacterial endosymbionts in Issidae using modern microscopy and molecular methods, and compare their identities to known symbionts from related insects. To do so, we will extract DNA from insect samples, amplify bacterial symbiont 16S genes, sub-clone PCR products, sequence the products, and determine their phylogenetic relationships to other insect symbionts and free-living bacteria. Our study …

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Prothonotary Warbler Nestling Growth And Condition Inresponse To Variation In Aquatic And Terrestrial Preyavailability, Jenna C. Dodson, Nicholas J. Moy, Lesley P. Bulluck Jan 2016

Prothonotary Warbler Nestling Growth And Condition Inresponse To Variation In Aquatic And Terrestrial Preyavailability, Jenna C. Dodson, Nicholas J. Moy, Lesley P. Bulluck

Biology Publications

Aquatic prey subsidies entering terrestrial habitats are well documented, but little is known about the degree to which these resources provide fitness benefits to riparian consumers. Riparian species take advantage of seasonal pulses of both terrestrial and aquatic prey, although aquatic resources are often over-looked in studies of how diet influences the reproductive ecology of these organisms. Ideally, the timing of resource pulses should occur at the time of highest reproductive demand. This study investigates the availability of aquatic(mayfly) and terrestrial (caterpillar) prey resources as well as the nestling diet of the prothonotary warbler (Protonotaria citrea) at two sites along …

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Neuroprotection Comparison Of Different Nutraceutical Compounds Against Mechanistically Distinct Cell Death Inducing Agents, Faten I. Taram Jan 2016

Neuroprotection Comparison Of Different Nutraceutical Compounds Against Mechanistically Distinct Cell Death Inducing Agents, Faten I. Taram

Electronic Theses and Dissertations

Neurodegenerative diseases like Alzheimer's disease (AD), Parkinson's disease (PD), and amyotrophic lateral sclerosis (ALS), include the progressive loss of structure and function of neurons leading to neuronal death. All of these diseases are fatal, as there is no cure for them. The causes of these diseases are unknown; however, there are many proposed mechanisms that lead to neurodegenerative diseases. Oxidative stress is the leading cause of cell death in neurodegenerative diseases, in addition to other mechanisms including endoplasmic reticulum stress, proteasome inhibition, nitrosative stress, inflammation and excitotoxicity. More understanding of the death mechanisms at work in neurodegeneration is necessary to …

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The High Cost Of Reproduction In Sea Otters Necessitates Unique Physiological Adaptations, Nicole Thometz, T. L. Kendall, B. P. Richter, T. M. Williams Jan 2016

The High Cost Of Reproduction In Sea Otters Necessitates Unique Physiological Adaptations, Nicole Thometz, T. L. Kendall, B. P. Richter, T. M. Williams

Biology Faculty Publications

Resting Superimposed on inherently high basal metabolic demands, the additional energetic requirements of reproduction can push female sea otters beyond physiological limits. Indeed, the resulting energy imbalance contributes to disproportionately high rates of mortality at the end of lactation in this species. To examine and quantify metabolic changes associated with reproduction, we measured the resting metabolic rate (RMR) of a female sea otter across gestation, lactation and non-reproductive periods. Concurrently, measurements were made on a non-breeding control female. Our results suggest that RMR declines during gestation. Conversely, RMR increases during lactation, reaches a peak at 3–4 months postpartum, and remains …

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Characterization Of Sterility And Germline Defects Caused By Smed-Boule Rna-Interference, Jessica Kathryne Steiner Jan 2016

Characterization Of Sterility And Germline Defects Caused By Smed-Boule Rna-Interference, Jessica Kathryne Steiner

Browse all Theses and Dissertations

Evolutionarily conserved molecular processes involved in construction of the germline and embryonic development are essential for the procreation of many species. Infertility affects 15% of couples in the world and can be caused by dysfunctions during egg and sperm development, anatomic defects, as well as faulty embryonic development. Although there are some infertility disorders that are genetically defined, such as Turner and Klinefelter syndromes, many clinical infertility cases are diagnosed as idiopathic due to the lack of understanding of basic fertility mechanisms. Schmidtea mediterranea is a freshwater planarian species that has the ability to regenerate complete organisms, including germ cells …

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Mucus Of Achatina Fulica Stimulates Mineralization And Inflammatoryresponse In Dental Pulp Cells, Fahsai Kantawong, Pichaporn Thaweenan, Sutinee Mungkala, Sawinee Tamang, Ruthairat Manaphan, Phenphichar Wanachantararak, Teerasak E-Kobon, Pramote Chumnanpuen Jan 2016

Mucus Of Achatina Fulica Stimulates Mineralization And Inflammatoryresponse In Dental Pulp Cells, Fahsai Kantawong, Pichaporn Thaweenan, Sutinee Mungkala, Sawinee Tamang, Ruthairat Manaphan, Phenphichar Wanachantararak, Teerasak E-Kobon, Pramote Chumnanpuen

Turkish Journal of Biology

Dental pulp tissue contains stem cells that can be isolated and used for regenerative medicine in tooth restoration or autologous transplantation. The aim of this study was to observe the mineralization and gene expression in dental pulp cells (DPCs) following treatment with snail mucus. Snail mucus was collected from adult Achatina fulica and processed as a dry powder by the freeze-drying technique. The mucus powder was dissolved in a culture medium at a concentration of 15 μg/mL. DPCs were prepared by the outgrowth technique and cultured in a 6-well plate at a density of 5 × 104 cells per well. …

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Single-Locus Versus Multilocus Patterns Of Local Adaptation To Climate In Eastern White Pine (Pinus Strobus, Pinaceae), Om P. Rajora, Andrew J. Eckert, John W. R. Zinck Jan 2016

Single-Locus Versus Multilocus Patterns Of Local Adaptation To Climate In Eastern White Pine (Pinus Strobus, Pinaceae), Om P. Rajora, Andrew J. Eckert, John W. R. Zinck

Biology Publications

Natural plant populations are often adapted to their local climate and environmental conditions, and populations of forest trees offer some of the best examples of this pattern. However, little empirical work has focused on the relative contribution of single-locus versus multilocus effects to the genetic architecture of local adaptation in plants/forest trees. Here, we employ eastern white pine (Pinus strobus) to test the hypothesis that it is the inter-genic effects that primarily drive climate-induced local adaptation. The genetic structure of 29 range-wide natural populations of eastern white pine was determined in relation to local climatic factors using both …

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Genome-Wide Analysis Of Response To Low Sulfur (Lsu) Genes In Grass Species And Expression Profiling Of Model Grass Species Brachypodium Distachyon Under S Deficiency, Hüseyi̇n Tombuloğlu, Abdugaffor Ablazov, Ertuğrul Fi̇li̇z Jan 2016

Genome-Wide Analysis Of Response To Low Sulfur (Lsu) Genes In Grass Species And Expression Profiling Of Model Grass Species Brachypodium Distachyon Under S Deficiency, Hüseyi̇n Tombuloğlu, Abdugaffor Ablazov, Ertuğrul Fi̇li̇z

Turkish Journal of Biology

Sulfur (S) affects the plant life cycle and crop yield and has nutritional importance for human and animal diet. Its deficiency is one of the major problems in agriculture. However, the plant-specific LSU (response to Low Sulfur) gene family has not been extensively analyzed in major plant species such as grasses. In this study, we have performed in silico genome-wide analysis of LSU genes in 6 grass species, including Brachypodium distachyon, Sorghum bicolor, Oryza sativa, Zea mays, Triticum aestivum, and Panicum virgatum. All identified LSU genes contained one exon encoding proteins of acidic character with cytoplasmic localization. In silico analysis …

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Skeletal Muscle Mass And Composition During Mammalian Hibernation, Clark J. Cotton Jan 2016

Skeletal Muscle Mass And Composition During Mammalian Hibernation, Clark J. Cotton

Biology Faculty Publications

Hibernation is characterized by prolonged periods of inactivity with concomitantly low nutrient intake, conditions that would typically result in muscle atrophy combined with a loss of oxidative fibers. Yet, hibernators consistently emerge from winter with very little atrophy, frequently accompanied by a slight shift in fiber ratios to more oxidative fiber types. Preservation of muscle morphology is combined with down-regulation of glycolytic pathways and increased reliance on lipid metabolism instead. Furthermore, while rates of protein synthesis are reduced during hibernation, balance is maintained by correspondingly low rates of protein degradation. Proposed mechanisms include a number of signaling pathways and transcription …

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Enhanced Salt Tolerance Of Transgenic Tobacco Expressing A Wheat Salt Tolerance Gene, Musa Kavas, Mehmet Cengi̇z Baloğlu, Ayşe Meral Yücel, Hüseyi̇n Avni̇ Öktem Jan 2016

Enhanced Salt Tolerance Of Transgenic Tobacco Expressing A Wheat Salt Tolerance Gene, Musa Kavas, Mehmet Cengi̇z Baloğlu, Ayşe Meral Yücel, Hüseyi̇n Avni̇ Öktem

Turkish Journal of Biology

Soil salinity is one of the most important limiting factors of agricultural productivity in the world. The Triticum aestivum salt tolerance-related gene (TaSTRG) possesses a functional response to salt and drought stress conditions. A variety of stress factors, such as salt, drought, abscisic acid, and cold, may induce the expression of TaSTRG in wheat. In this study, the TaSTRG gene was transferred to tobacco via Agrobacterium-mediated transformation. Overexpression of TaSTRG in transgenic tobacco plants indicated higher salt tolerance and mediated more vigorous growth than in wild-type plants. Under salt stress conditions, the transgenic tobacco plants had higher germination and survival …

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Calorie Restriction Effect On Circadian Clock Gene Expression, Sonal Arvind Patel Jan 2016

Calorie Restriction Effect On Circadian Clock Gene Expression, Sonal Arvind Patel

ETD Archive

Calorie Restriction (CR) is a powerful paradigm known to delay aging and thus increase longevity in several organisms, from yeast to non-human primates. Many molecular pathways have been proposed to mediate the beneficial effects of CR, however, the mechanism is still unknown. Circadian clock which is an internal time keeping system is regulated by feeding. Thus our aim was to study the effect of CR on the circadian clock. Here we show that CR significantly affects the expression of circadian clock genes in mice at the mRNA and protein levels, suggesting that CR reprograms the clocks at the transcriptional and …

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Marked Variation Between Winter And Spring Gut Microbiota In Freeranging Tibetan Macaques (Macaca Thibetana), Binghua Sun, Xi Wang, Sofi Bernstein, Michael A. Huffman, Dong-Po Xia, Zhiyuan Gu, Rui Chen, Lori K. Sheeran, R. Steven Wagner, Jinhua Li Jan 2016

Marked Variation Between Winter And Spring Gut Microbiota In Freeranging Tibetan Macaques (Macaca Thibetana), Binghua Sun, Xi Wang, Sofi Bernstein, Michael A. Huffman, Dong-Po Xia, Zhiyuan Gu, Rui Chen, Lori K. Sheeran, R. Steven Wagner, Jinhua Li

Anthropology and Museum Studies Faculty Scholarship

Variation in the availability and distribution of food resources is a strong selective pressure on wild primates. We explored variation in Tibetan macaque gut microbiota composition during winter and spring seasons. Our results showed that gut microbial composition and diversity varied by season. In winter, the genus Succinivibrio, which promotes the digestion of cellulose and hemicellulose, was significantly increased. In spring, the abundance of the genus Prevotella, which is associated with digestion of carbohydrates and simple sugars, was significantly increased. PICRUSt analysis revealed that the predicted metagenomes related to the glycan biosynthesis and metabolic pathway was significantly increased in winter …

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