Digital Commons Network^™

A Chronic Toxicity Assessment Of An Industrial Effluent Utilizing Pimephales Promelas And Ceriodaphnia Sp, Sharon J. Whitaker Fugate

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Arts and Sciences at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Sharon J. Whitaker Fugate on July 23, 1990.

The Electrokaryotype And Molecular Characterization Of Trypanosoma Cervi Isolates Using Recombinant Dna Techniques, J. Lindsley Bennett

Dissertations and Theses

The distribution of trypanosomes infecting wild ruminants of North America has only recently been investigated. Many isolates have been mensurally studied and were determined to be conspecific with Trypanosoma cervi. Widely divergent forms exist however, between host species and seasonally within a host. To determine the validity of all inclusions in the taxon and the extent of intraspecific variability, trypanosome isolates of moose, reindeer, antelope, muledeer, Roosevelt Elk and two discrete transplant populations of Rocky Mountain elk were characterized and differentiated using recombinant DNA techniques.

Reproduction In The Hispid Cotton Rat, Sigmodon-Hispidus Say And Ord (Rodentia: Muridae), In Southeastern Virginia, Robert K. Rose, Michael H. Mitchell

Biological Sciences Faculty Publications

The hispid cotton rat, Sigmodon hispidus Say and Ord, a species of the southwestern United States that has been moving northward and eastward in this century, was first observed in Virginia in 1940. In this study of the cotton rat in southeastern Virginia, most males were reproductively competent from February through November, embryos were recorded from March through October, and litter sizes were comparable to those from other locations except Kansas. Also unlike the cotton rat in Kansas, animals grew at substantial rates during the winter in Virginia. The hispid cotton rat seems to have adjusted its breeding season in …

Evidence Of A Neurogenic Component During Ige-Mediated Inflammation In Murine Skin, Victoria Jean Cavanaugh

Biological Sciences Theses & Dissertations

The direct stimulation of sensory nerves or the intradermal injection of neuropeptides triggers a cutaneous inflammatory response that involves the degranulation of mast cells. These neurogenic inflammatory reactions are physiologically similar to IgE-mediated hypersensitivity responses, which also depend on mast cells. Although non-antigenic stimuli distinguish neurogenic from IgE-mediated inflammation, the similarity of their effector stages suggested that peripheral nerves may also participate in lgE-mediated responses. To examine this hypothesis, IgE responses were elicited in denervated skin. A murine model was developed where the footpad was denervated by surgically removing a 2 mm segment of the sciatic nerve, proximal to the …

Small Mammals In The Great Dismal Swamp Of Virginia And North Carolina, Robert K. Rose, Roger K. Everton, Jean F. Stankavich

Biological Sciences Faculty Publications

Small" mammals were surveyed in a range of habitats in the Great Dismal Swamp of Virginia and North Carolina. The survey is based on three chronologically overlapping studies, each lasting 15-18 months and for which the results have been reported separately. A different trapping method was used in each of the three studies: nest boxes, Fitch live traps, or pitfall traps. Only two species of mammals, both arboreal, were taken in nest boxes, compared with 10 and 9 species in Fitch live traps and pitfall traps, respectively. The Fitch live traps had a much higher catch rate per 1,000 trap-nights …

Larval Settlement Of Ilyanassa Obsoleta (Gastropoda) And Capitella Sp. I (Polychaeta): Responses To Sediment Cues And Effects Of No. 6 Fuel Oil, Dianne Rudy '90

Honors Projects

Metamorphosis in response to bottom sediment was measured in two species of marine planktonic larvae. Ilyanassa obsoleta, a common mud-snail, and Capitella sp. I, a polychaete worm. A sediment control consisted of organic-rich sediment collected from Barnstable Harbor, Ma. One sediment treatment, termed muffled sediment, consisted of the sediment control heated at a high temperature in a muffle oven to remove all organic matter. A sharp decrease in percent metamorphosis was observed in larvae exposed to the muffled sediment condition compared to the sediment control. Results confirmed that settlement involves more than physical contact with the sediment and suggest that …

Description Of The Tadpoles Of Gastrophryne Pictiventris And Nelsonophryne Aterrima (Anura: Microhylidae), With A Review Of Morphological Variation In Free-Swimming Microhylid Larvae, Maureen A. Donnelly, Rafael O. De Sá, Craig Guyer

Biology Faculty Publications

The tadpoles of Gastrophryne pictiventris and Nelsonophryne aterrima are described based on material collected in Costa Rica and Panama, respectively. The tadpole of G. pictiventris resembles those of the other species of Gastrophryne. The tadpole of N. aterrima differs from other New World microhylid tadpoles in location of the vent tube (medial), the spiracular opening (ventral to the gut), and eyes (dorsolateral). The unique configuration of mouthparts in N. aterrima is described. Morphological variation in microhylid tadpoles is reviewed and six larval characters are analyzed cladistically for IO New World microhylid genera.

Temporal Changes In The Ommatidial Structure Of The Cockroach, Leucophaea Maderae, Becky Reitcheck

Masters Theses & Specialist Projects

A circadian rhythm in eye sensitivity to light has been previously reported for Leucophaea maderae. Temporal changes in eye cell morphology that could be correlated with those changes in eye sensitivity to light were examined. Rhabdom area, screening pigment organization and palisade layer area about the rhabdom were the parameters measured to detect structural change through time. Measurements of those parameters from tissue samples obtained from the anterior one-third of compound eyes surgically removed at midday, light offset, midnight and light onset from roaches entrained to a 12-h light / 12-h dark photoperiodic cycle were used to assess the …

Chromatin Structure And Gene Activity, Sarah C.R. Elgin

Biology Faculty Research

No abstract provided.

Fisheries Research Report No 88 - A Synopsis Of The Biology And The Exploitation Of The Australiasian Pilchard, Sardinops Neopilchardus (Steindachner) Part 1: Biology, W. J. Fletcher

Fisheries Research Reports

There have been a large number of studies which relate to the biology and exploitation of the Australian pilchard, Sardinops neopilchardus. Much of the data, however, are located in journals which are hard to obtain, or in unpublished reports, and theses. With the increase in catch of pil­chards, particularly in Western Australia, a collation of all the available knowledge pertaining to pilchards in Australia and New Zealand was desired to help with the planning of future research into the Western Australian stocks of this species. This report contains all the information located on the biology of Sardinops neopilchardus, a companion …

Enhancement And Monitoring Of The Procambarus Clarkii Population In Lake Mead, Mikell Beth Hager

Publications (WR)

Procambarus clarkii are found in extremely low numbers throughout Lake Mead, AZ-NV. The crayfish are an important dietary component for game fish. Enhancement of the crayfish population would broaden the fishery forage base. Crayfish were stocked and monitored in a study cove on Saddle Island to determine if the Procambarus clarkii population could be enhanced. A trapping survey of the area after the following reproductive season yielded low numbers of crayfish. A comparison of pre-stocking and post-stocking catch per trap day (CPTD) values revealed no significant increase in the population. Procambarus clarkii growth is limited by environmental factors in Lake …

Immunological Effects Of Ketoconazole, Itraconazole And Fluconazole On Lymphocyte Cell Proliferation And Natural Killer Cell Activity In Immune-Normal, Cyclosporine-Compromised And Cyclophosphamide-Compromised Mouse Models, Jeffrey Tullis Mitchell

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Over the past several years there has been a steady increase in the incidence of immunologically compromised patients. This has been the result of both chemical agents, such as those used in cancer chemotherapy, and biological agents such as HIV, the cause of Acquired lmmunodefeciency Syndrome (AIDS). The increase in immune-suppressed patients has lead to an increase in life-threatening mycoses requiring treatment with antifungal agents. Pharmaceutical companies have increased research for the development of new antifungal agents which are more effective and less toxic than those that are currently used. Several researchers have reported on antifungal agents that demonstrate both …

The Effects Of Intracerebral Injections Of Substance P, And A Substance P Fragment (5-11) Into The Medial Preoptic Nucleus On Lordosis Behavior In The Female Rat, Kellie S. Jones '90

Honors Projects

In this study either substance P (SP), SP 5-11, an analogue resistant to enzymatic degradation, or acidified saline was injected bilaterally into the medial preoptic nucleus (MPN) of the medial preoptic area (MPOA) in ovariectomized (ovx) estrogen-primed female rats. The lordosis quotient (LQ) and the lordosis intensity scale (LIS) were recorded using a steroid regime that produced a slow increase of sexual receptivity. In experiment 1, when receptivity was low, bilateral injections of 2 different doses of SP (100 ng,1000 ng/ cannula), had no effect on lordosis behavior when compared to those injected with acidified saline. In experiment , however, …

Comparison Of The Protein Content In Selected Tissues At Different Feeding Stages In The American Dog Tick Dermacentor Variabilis (Say), Mark John Beveridge

Biological Sciences Theses & Dissertations

Selected tissues from Dermacentor yariabilis (Say) were collected at different feeding stages (unfed, part-fed, and replete) and examined by several different protein analyses to determine soluble protein content, protein or polypeptide molecular weights, and pI values. Results showed a relationship between the salivary gland, ovary and hemolymph and evidence indicates the synganglion produces and secretes substances that may control oocyte development.

'Eve' In Africa: Human Evolution Meets Molecular Biology, Robert D. Seager

Faculty Work

Recent advances in the study of human origins have increased our understanding of our ancestors. There have been new, major fossil finds. WT 17000, a 2.5 million-year-old robust Australopithecus found in Kenya (Walker et al. 1986), led to a revision of early hominid phylogeny (Delson 1986; 1987). Existing fossil materials have been reassessed. For example, Tattersall (1986) maintains that at least two unrecog­- nized hominid species (Homo neanderthalensis, H. hei­ delbergensis and possibly H. steinheimensis ) existed be­ tween the times of H . erectus and fully modern H . sapiens.

Multielement Analysis Of Biological Materials By Particle-Induced X-Ray Emission (Pixe), Willy Maenhaut

Scanning Microscopy

An analytical particle-induced X-ray emission (PIXE) procedure for the multielement analysis of biological materials consists of various stages. These include sample and specimen preparation, specimen bombardment, spectral data processing, quantification and correction for matrix effects. Critical aspects of the procedure are contamination and/or losses during sample and specimen preparation and the danger of radiation or heat-induced losses during specimen bombardment. With optimized PIXE procedures precisions of 1-2% and an accuracy of better than 5% are obtainable, whereas the detection limits are down to 0.1 μg/g. Because of its inherent characteristics, PIXE offers great potential for trace element analysis in the …

Scanning Electron Microscope Study Of Wohlfahrtia Magnifica (Schiner, 1862) (Diptera: Sarcophagidae) I. Structures With Parasitic And Possible Taxonomic Meaning, I. Ruíz-Martínez, M. D. Soler-Cruz, R. Benítez-Rodríguez, J. M. Pérez-Jiménez, C. Adalid-Fuentes, M. Díaz-López

Scanning Microscopy

The larval development of Wohlfahrtia magnifica (the most important dipteran causing sheep myiasis in the Palearctic region) has been studied by means of scanning electron microscopy. The ultrastructure of mouth-hooks, oral ridges, labial lobes, body spines and anterior and posterior peritremes is described for the first time. Their possible adaptations to a parasitic lifeway are also discussed. Thus, the use of new structures in the Sarcophagidae taxonomy is proposed from the point of view of their ultrastructure and adaptative morphology.

Responses Of Astrocytes In Culture After Low Dose Laser Irradiation, David T. Yew, D. R. Zheng, C. Au, W. W. Y. Li

Scanning Microscopy

The effect of Helium-Neon low dose laser on astrocytes was investigated in cultures of isolated astrocytes from albino neonatal rats. The laser appeared to inhibit the growth of astrocytes as exemplified by the smaller sizes of the cells and the decreased leucine uptake in each cell after treatment. Temporary decrease in the number of mitoses was also observed, but this trend was reversed soon after. Electron microscopic studies revealed an increase in buddings from cell bodies and processes (branches) after irradiation.

Bird Flight - Insights And Complications, G. E. Goslow Jr., Kenneth P. Dial, F. A. Jenkins Jr.

Biological Sciences Faculty Publications

No abstract provided.

Optimal Heat-Induced Expression Of The Drosophila Hsp26 Gene Requires A Promoter Sequence Containing (Ct)N.(Ga)N Repeats, R Glaser, Graham H. Thomas, Ester Siegfried, Sarah C.R. Elgin, J Lis

Biology Faculty Research

We report here the analysis of the sequence requirements for the heat-induced expression of the Drosophila melanogaster hsp26 gene using germline transformation. Heat-induced expression is augmented fivefold by a homopurine/homopyrimidine region from -85 to -134 that is devoid of heat-shock elements but contains numerous (dC-dT).(dG-dA) repeats. Sequences within this interval have been shown to assume a nuclease S1-hypersensitive structure in vitro. In this paper, we extend those in vitro observations, demonstrating that the S1-hypersensitive structure is triple-helical H-DNA formed by a symmetric (dC-dT).(dG-dA) sequence. Thus, the sequences that form H-DNA in vitro are also required in vivo for optimal hsp26 …

Particle Induced X-Ray Emission Elemental Analysis: Sample Preparation For A Versatile Instrumental Method, N. F. Mangelson, M. W. Hill

Scanning Microscopy

Particle induced X-ray emission (PIXE) has found extensive application as a multi-element analysis tool. The energetic nature of both the incident particle beam and the emitted characteristic X-rays has lead to considerable versatility in regard to samples which may be analyzed by the PIXE technique. This paper does not deal with the theory, equipment, or data analysis for PIXE, but it should bring to the reader a broad perspective of methods for sample preparation and should point the way into the literature where more complete details are given. Both physical and chemical processes for sample preparation are outlined. Preparation often …

Evidence That A Major Portion Of Cellular Potassium Is "Bound", Ivan L. Cameron, W. Elaine Hardman, Keithley E. Hunter, Christine Haskin, Nancy K. R. Smith, Gary D. Fullerton

Scanning Microscopy

In this report we briefly review recent evidence which shows that a substantial proportion of intracellular K⁺ is "bound" or perturbed from the physicochemical properties expected in dilute aqueous solutions. In addition, we present evidence from electron probe x-ray microanalysis of thin cryosections of cells which indicates that the binding of K⁺ to anionic groups either carboxyl groups (HCO₂) on proteins or to phosphate groups in creatine phosphate (CrP) , in adenosine triphosphate, (ATP), in protein and in nucleic acids, are the main determinants of the maintenance of (as differentiated from the generated of) the well …

Dna Fingerprinting Analysis Of Captive Asian Elephants, Elephas Maximas, Laura Louise Bischof

Dissertations and Theses

This thesis examined the effectiveness of DNA fingerprinting analysis for paternity ascertainment and the establishment of relatedness of captive Asian elephants (Elephas maximas). Eighteen Asian elephants from three North American zoos were examined. Thirteen of these elephants were wild caught. Relationships between these elephants and the remaining elephants born in captivity were known.

Changes In Rat And Mouse Salivary Glands And Pancreas After Chronic Treatment With Diuretics: A Potential Animal Model For Cystic Fibrosis, Samuel Sagström, Edward B. Mcmillan, Monique Marijianowski, Helmy Mulders, Godfried M. Roomans

Scanning Microscopy

Defective transepithelial chloride and water transport is thought to be the cellular basis of the disease cystic fibrosis (CF). Therefore, it was attempted to develop an animal model for this disease by chronically inhibiting transepithelial chloride transport in experimental animals by long term treatment with high doses of diuretics. In the present study, changes in the salivary glands and pancreas after such treatment were investigated by X-ray microanalysis and electron microscopy.

Treatment of rats for one month with diuretics caused a significant decrease in chloride and an increase in calcium in the acinar cells of the submandibular gland. This increase …

Studies Of Irritant Reactions On Epidermis: The Application Of Energy Dispersive X-Ray Microanalysis, Magnus Lindberg

Scanning Microscopy

Modern man is exposed to an increasing amount of chemicals in the environment. The skin is a complex tissue with regards to both structure and function. Contact with external stimuli can lead to various responses in the skin depending on the type of stimuli, the dose and time of exposure and the status of the exposed individual. The electron probe microanalytic technique can be used to obtain information on physiological alterations within the skin and especially the epidermis when the normal homeostasis is disturbed by contact with external stimuli. The present paper presents a review of some aspects of contact …

Correlative Light, Transmission, And High Resolution (Se-I) Scanning Electron Microscopy Studies Of Rhesus Adrenocortical Vascular Morphology, R. P. Apkarian, N. L. L' Hernault

Scanning Microscopy

A detailed correlative morphologic description using light microscopy (LM), transmission electron microscopy (TEM) and high resolution SE-I scanning electron microscopy (SEM) was conducted on the capillary endothelium of the zona-fasciculata (Z-F) in juvenile male rhesus monkeys. The glucocorticoid synthesis and release phenomena, associated with stress stimulated release of adrenocorticotropic hormone (ACTH) via the hypothalamic-pituitary axis, intimately involves capillaries of the Z-F. A comprehensive study of all the ultrastructural features implicated in the transendothelial uptake of steroidogenic precursors and release of glucocorticoids in perfused rhesus adrenals has not previously been made. This report presents correlative images of transendothelial openings that include …

Comparative Analysis Of The Proliferative Response Of The Rat Urinary Bladder To Sodium Saccharin By Light And Scanning Electron Microscopy And Autoradiography, Samuel M. Cohen, Maria J. Fisher, Takao Sakata, Martin Cano, Gerald P. Schoenig, Clifford I. Chappel, Emily M. Garland

Scanning Microscopy

Three methods used to detect proliferative changes in the rat urothelium, light microscopy, scanning electron microscopy, and autoradiography, were compared for their sensitivity in detecting changes produced by administration of sodium saccharin. Weanling male F344 rats were fed sodium saccharin as 0, 3, 5, or 7.5% of the diet, and the bladders were evaluated after 4, 7, and 10 wks of feeding. Light microscopic changes and an increase in labeling index were seen at all time points in rats fed 7.5 % sodium saccharin, but not at the lower doses. A slight increase in labeling index was also observed at …

The Genera Of Arundinoideae (Gramineae) In The Southeastern United States, Gordon C. Tucker

Faculty Research & Creative Activity

No abstract provided.

Food Quality, Heterozygosity, And Fitness Correlates In Peromyscus Polionotus, James M. Novak, William R. Teska, Michael H.. Smith

Faculty Research & Creative Activity

Oldfield mice (Peromyscus polionotus) that are more heterozygous utilize food and maintain body weight under varying degrees of dietary stress better than their less heterozygous counterparts. Mice were collected in southern Florida and fed diets of three qualities. During each dietary treatment, body weight, amount of food eaten, amount of food absorbed, and feeding efficiency were determined. Body weights for all mice decreased during the experiment. More heterozygous mice maintained their weight better during periods of dietary stress than those that were less heterozygous. Mice with different levels of genetic variability had essentially the same mean feeding efficiency with high …

Heat Dissipation, Gas Exchange And Acid-Basic Balance In The Land Snail Oreohelix During Short-Term Estivation, Bernard Rees

Biological Sciences Faculty Publications

Within 4 days following entry into estivation, heat dissipation and oxygen consumption by the land snail Oreohelix spp. decreased by 83 % compared to standard non-estivating rates. During both non-estivating and estivating conditions, the quantity of heat dissipated per mole of O2 consumed was indicative of a completely aerobic metabolism. This calorimetric-respirometric (C/R) ratio was — 461 ± 12 kJ moP102 (S.E.M., N=5) under standard non-estivating conditions and — 464±26kJmol~1O2 (7V=4) during estivation. Respiratory exchange ratios reflected a primary dependence upon carbohydrate as a metabolic substrate during both states. Carbon dioxide retention occurred during the first 36h of estivation, resulting …