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Mechanism And Control Of Beef Toughening During Retail Display In High Oxygen Modified Atmosphere Packages, Lasika S. Senaratne

Department of Animal Science: Dissertations, Theses, and Student Research

This research was conducted to elucidate the mechanism and factors (diet, aging time, antioxidant level, and retail display time) affecting beef toughening in retail display under high oxygen atmosphere packages (HiOx-MAP; 80% O₂ and 20% CO₂). In the first study, effects of HiOx-MAP on color and lipid stability of beef m. longissimus lumborum steaks from yearlings fed corn or 30% wet distillers grains plus soluble (WDGS) diets supplemented with (150 mg/kg) or without (0 mg/kg) AGRADO-PLUS (AG) antioxidants were studied. Steak color-shelf life was extended (P < 0.0001) by HiOx-MAP compared to oxygen permeable film packages (PVC-OW). Steaks from corn+AG-supplemented cattle had (P ≤ 0.05) less discoloration and lower lipid oxidation levels …

Post-Fire Soil Water Repellency: Extent, Severity And Thickness Relative To Ecological Site Characteristics Within Piñon-Juniper Woodlands, Daniel Lewis Zvirzdin

Theses and Dissertations

Erosion and weed dominance often limit the recovery of burned piñon-juniper woodlands. Soil water repellency (SWR) is one factor that may contribute to this by increasing overland flow and impeding seedling establishment. In spite of these effects, the extent of SWR within piñon-juniper woodlands is unknown. In this study, the extent, severity and thickness of SWR were sampled across 41 1,000 m2 plots within three 2009 Utah wildfires. Predictive models of SWR were built from ecological site characteristic data collected at each site. Across the study, SWR was found at 37% of the points sampled. SWR extent was strongly related …

A Study Of The Eastern Oyster, Crassostrea Virginica, In Tampa Bay: Effects Of Perkinsus Marinus On Reproduction And Condition, Bridgit Melora Mccrickard

USF Tampa Graduate Theses and Dissertations

Abstract

Five sites in Tampa Bay, Florida, were sampled monthly from February 2006 to January 2007. These sites were located at the mouth of the Alafia River, in an inlet of Cross Bayou, on the easement of Gandy Bridge, near mangrove in Manatee County, and an inlet of Salt Creek, off Bayboro Harbor. Standard methods were used to determine shell height and Condition Index (CI). Intensity and prevalence of Perkinsus marinus were analyzed using Ray's Thioglycollate medium test, while Haplosporidium nelsoni was studied by histological examination. Histological methods were also used to determine sex ratios, reproductive phases, and egg diameters. …

Fecal Near Infrared Spectroscopy To Discriminate Physiological Status In Giant Pandas., Erin E. Wiedower, Andrew J. Kouba, Carrie K. Vance, Rachel L. Hansen, Jerry W. Stuth, Douglas R. Tolleson

CALS Publications

Giant panda (Ailuropoda melanoleuca) monitoring and research often require accurate estimates of population size and density. However, obtaining these estimates has been challenging. Innovative technologies, such as fecal near infrared reflectance spectroscopy (FNIRS), may be used to differentiate between sex, age class, and reproductive status as has been shown for several other species. The objective of this study was to determine if FNIRS could be similarly used for giant panda physiological discriminations. Based on samples from captive animals in four U.S. zoos, FNIRS calibrations correctly identified 78% of samples from adult males, 81% from adult females, 85% from adults, 89% …

Genetic Comparison Of Water Molds From Embryos Of Amphibians Rana Cascadae, Bufo Boreas And Pseudacris Regilla, Kori K. Ault, James E. Johnson, Holly C. Pinkart, R. Steven Wagner

All Faculty Scholarship for the College of the Sciences

Water molds that cause the disease saprolegniasis have been implicated in widespread mortality of amphibian embryos. However, because of the limitations of traditional identification methods, water mold species involved in die-offs or utilized in ecological studies often remain unidentified or identified only as Saprolegnia ferax. Furthermore, water mold taxonomy requires revision, so very distinct organisms may all be called S. ferax. Recent DNA-based studies indicate that the diversity of water molds infecting amphibian embryos is significantly higher than what was previously known, but these studies rely on culture methods, which may be biased towards taxa that grow best …

Climate's Role In Polar Bear Past [Letters], Kurt E. Galbreath, Joseph A. Cook, Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Striking temporal concordance between the new date for divergence of polar bears and persistent freezing of the Arctic Ocean suggests that this may be one of relatively few instances in which a specific paleoclimatological episode can be convincingly linked to a specific evolutionary event, and it provides vivid demonstration of climatic forcing as a determinant of diversification in biological systems.

Evaluating The Potential Use Of Change-In-Ratio And Index Removal Techniques For Determining Harvest Rates And Efficiency Increases In The Western Rock Lobster Fishery Frdc Project 2009/019, Simon De Lestang, J Hoeing, Stuart Frusher, Norman G. Hall

Fisheries Research Reports

Objectives: 1. Assess current data sources and their potential for estimating harvest rates and increases in efficiency in the western rock lobster fishery using change in ratio and index removal techniques. 2. Evaluate whether additional sources of information are needed to produce more robust estimates of harvest rate and efficiency increase. 3. Assess whether the estimates of harvest rate and fishing efficiency are reliable and could be used for the management of the western rock lobster fishery.

Aquatic Animal Health Subprogram: Development Of A Dna Microarray To Identify Markers Of Disease In Pearl Oysters (Pinctada Maxima) And To Assess Overall Oyster Health: Final Frdc Report – Project 2008/030, David Raftos, Sham Nair, Melanie Croxford, Brian J. Jones

Fisheries Research Reports

Objectives: 1. To construct a cDNA library using healthy and stressed oysters. 2. To design and print DNA microarray slides for the analysis of diseased states in pearl oysters (P. maxima). 3. To use the DNA microarray to identify molecular markers that differentiate between pearl oysters that are stressed from those that appear to be healthy. To use the DNA microarray to test for markers of adverse health in pearl oysters that appear to be affected by environmental stressors other than OOD.

Policy On Habitat Enhancement Structures In Western Australia, Department Of Fisheries Western Australia.

Fisheries Management Papers

Habitat enhancement structures range widely in design. They generally aim to enhance fish habitat by providing a structure for fish and other aquatic life to colonise and potentially lead to an increase in their biological production.

The primary function of an artificial reef is to provide additional structure and habitat to support an increased production of fish ad other aquatic organisms.

Aquatic Heteroptera Of The Lake Manguao Catchment, Palawan And New Rank Of Rhagovelia Kawakamii Hoberlandti Hungerford & Matsuda 1961, Hendrik Freitag, Herbert Zettel

Biology Faculty Publications

Results of an inventory of the fauna of aquatic and semiaquatic true bugs (Insecta: Hemiptera) of small streams in a lake catchment of northern Palawan are presented. Twenty-one species were recorded. Taxonomic and ecological notes, distribution and collection sites are given for each identified taxon. Rhagovelia hoberlandti Hungerford & Matsuda 1961 is newly ranked as a subspecies of R. kawakamii (Matsumura 1913): Rhagovelia kawakamii hoberlandti Hungerford & Matsuda 1961, stat.n. Cercotmetus asiaticus Amyot & Serville 1843 is recorded for the first time from the Philippines. Endemic and few undescribed taxa are discussed. Additional environmental data of the sampled waters are …

Exploring The Houtman Abrolhos Islands, Western Australia. Department Of Fisheries

Fisheries Occasional Publications

This booklet is designed to assist you in exploring and appreciating the Houtman Abrolhos Islands. It provides a general overview on the attributes of the Abrolhos that make it so unique. A place rich in ecological, historical, economic and cultural significance for Western Australia.

Apart from being an introduction to the Abrolhos, the booklet also provides background and context for the various management plans and strategies developed and implemented to sustainably manage these Islands and their surrounding waters.

Developmental Programming In Response To Intrauterine Growth Restriction Impairs Myoblast Function And Skeletal Muscle Metabolism, Dustin T. Yates, A.R. Macko, M. Nearing, X. Chen, R.P. Rhoads, Sean W. Limesand

Department of Animal Science: Faculty Publications

Fetal adaptations to placental insufficiency alter postnatal metabolic homeostasis in skeletal muscle by reducing glucose oxidation rates, impairing insulin action, and lowering the proportion of oxidative fibers. In animal models of intrauterine growth restriction (IUGR), skeletalmuscle fibers have lessmyonuclei at birth. This means thatmyoblasts, the sole source formyonuclei accumulation in fibers, are compromised. Fetal hypoglycemia and hypoxemia are complications that result from placental insufficiency. Hypoxemia elevates circulating catecholamines, and chronic hypercatecholaminemia has been shown to reduce fetal muscle development and growth. We have found evidence for adaptations in adrenergic receptor expression profiles in myoblasts and skeletal muscle of IUGR sheep …

Validation Of Pcr-Based Assays And Laboratory Accreditation For Environmental Detection Of Aquatic Invasive Species, Invasive Species Advisory Committee

National Invasive Species Council

This white paper provides:

a) Background information on the use, accuracy and reliability of PCR-based assays such as environmentally sampled DNA (eDNA) for early detection of aquatic invasive species (AIS) and;

b) Recommendations for establishing a system for validating assays and accrediting laboratories that report on the presence or absence of AIS.

This white paper was developed by the members of ISAC and discusses the need for developing validation requirements for Polymerase Chain Reaction (PCR) and other DNA-based molecular assays that are increasingly being used to detect AIS. It does not provide a simplified checklist for evaluation of their ability …

Invasive Species And E-Commerce, Invasive Species Advisory Committee

National Invasive Species Council

ISSUE

Internet commerce (hereafter e-commerce) is a growing and vital part of the United States economy. Total e-commerce sales in the United States for 2011 totaled $194 billion, an increase of 16% over 2010. From 2002 to 2011, the proportion of reported e-commerce sales in the U. S. grew from about 1.4% to 5.5% of total retail sales (United States Census Bureau News 2012). Globally, e-commerce is expected to increase at a rate of 13.5% annually, amounting to $1.4 trillion in yearly sales by 2015 (Enright 2011). A portion of this activity includes the sale and trade of living organisms. …

Energy-Saving Hypothermia Reduces Flight Ability In Mourning Doves, Jennie M. Carr, Steven L. Lima

Symposium 2012

Overwintering birds are frequently exposed to thermal challenges that can quickly decrease energy reserves, thus leading to an increase in the risk of starvation. During these periods of energetic hardship, many avian species use nocturnal hypothermia to conserve energy that would otherwise be lost remaining warm throughout the night (McKechnie & Lovegrove 2002). However, a cool body temperature (Tb) may limit a bird’s ability to monitor the environment and may slow their response to a potential threat. Thus, birds likely trade-off between the benefits of energy-saving hypothermia and the potential costs of reduced behavioral responsiveness to predators (Welton et al. …

Fish Assemblage Dynamics And Red Drum Habitat Selection In Bayou St. John And Associated Urban Waterways Located Within The City Of New Orleans, Louisiana, Patrick W. Smith Mr.

LSU New Orleans Theses and Dissertations

Bayou St. John (BSJ) and City Park Lakes and Lagoons (CPLL) are urban waterways in New Orleans, Louisiana. I studied habitat selection of red drum in BSJ, and fish assemblage change in BSJ and CPLL over 40 years. Temperature was found to be the best predictor of red drum habitat selection in Bayou St. John, while salinity and change in depth also were found to be good predictors for certain sites. Potential prey item abundance did not appear to influence habitat selection. Using data from 1971 – 2010, nearshore habitats in CPLL were affected by Hurricane Katrina, but have sense …

Various Gaits Of African Elephant Calves, Elizabeth Anne Jennings

Undergraduate Honors Thesis Collection

It is commonly believed that elephants exhibit only one gait, a lateral sequence walk. However, recently there have been several interesting studies on the footfall patterns of adult African elephants (Loxodonta africana). Scientists have recognized that elephants actually exhibit two patterns of locomotion: a lateral sequence walk and an amble. The amble is a slight modification of the lateral sequence gait. The present study extends this work by examining the relationship between gait and speed in African elephant calves at the Indianapolis Zoo. I collected frame-by-frame observational video data from a newborn calf born at the Indianapolis Zoo across a …

Effect Of Plant-Derived Molecules On Acinetobacter Baumannii Biofilm On Abiotic Surfaces, Ryan P. Pelletier

Honors Scholar Theses

The emergence of multiple drug resistant (MDR) Acinetobacter baumannii has lead to an increased interest in finding alternative antimicrobial compounds for controlling the pathogen. The present study investigated the effect of three plant-derived antimicrobials, namely eugenol (EUG), carvacrol (CAR), and thymol (THY), on A. baumannii biofilms. Using concentrations equal to or greater than the minimum bactericidal concentration (MBC) of each compound, the ability of EUG, CAR, and THY to inactivate the mature biofilms of three strains of MDR A. baumannii on polystyrene microtiter plates and stainless steel coupons was examined. All three plant-derived compounds significantly inactivated the bioflims of all …

An Approach For Use Of Dual Frequency Identification Sonar (Didson) To Quantify Behavioral Aspects Of Piscivory At Ecologically Relevant Time And Space Scales, Victoria E. Price

Master's Theses

Predator-prey interactions of large vagile fishes are difficult to study in the ocean due to limitations in the space and time requirements for observations. Small-scale direct underwater observations by divers (<10m >radius) and large-scale hydroacoustic surveys (10s - 100s km2) are traditional approaches. However, large piscivorous predators identify and attack prey at the scale of meters to tens of meters. Dual- Frequency Identification Sonar, or DIDSON, is a high-resolution acoustic camera operating in the MHz range that provides detailed continuous video-like imaging of objects out to 30 m range. This technology can be used to observe predator-prey interactions at ecologically …

Terrestrial Habitat Requirements Of Nesting Freshwater Turtles, D. A. Steen, J. P. Gibbs, K. A. Buhlman, J. L. Carr, B. W. Compton, J. D. Congdon, J. S. Doody, J. C. Godwin, Kerry L. Holcomb, D. R. Jackson, F. J. Janzen, G. Johnson, M. T. Jones, J. T. Lamer, T. A. Langen, M. V. Plummer, J. W. Rowe, R. A. Saumure, J. K. Tucker, D. S. Wilson

All Faculty Scholarship for the College of the Sciences

Because particular life history traits affect species vulnerability to development pressures, cross-species summaries of life history traits are useful for generating management guidelines. Conservation of aquatic turtles, many members of which are regionally or globally imperiled, requires knowing the extent of upland habitat used for nesting. Therefore, we compiled distances that nests and gravid females had been observed from wetlands. Based on records of > 8000 nests and gravid female records compiled for 31 species in the United States and Canada, the distances that encompass 95% of nests vary dramatically among genera and populations, from just 8 m for Malaclemys to …

Ars Assists In Fight Against Kudzu Bug, Jan Suszkiw, Walker Jones

Agricultural Research Magazine

Sure, this distant relative of the brown marmorated stink bug will feed voraciously on the stems of kudzu, the “Vine That Ate the South.” But Megacopta cribraria also has a taste for soybean and other legumes. In Georgia, where this native of Asia was first discovered in the United States in October 2009, there’s worry that the pest will set its sights on peanut, endangering a $2 billion crop that supplies nearly 50 percent of America’s peanuts (Georgia Peanut Commission, 2009).

Like the brown marmorated stink bug, Megacopta—also known as the “bean plataspid”—seeks shelter inside homes, buildings, and vehicles …

Agricultural Research Magazine May-June 2012

Agricultural Research Magazine

Agricultural Research

150 Years of Making History

USDA's 150th Anniversary- May 15, 2012

Big Blue And You: An Interdisciplinary Look At Science And The Ocean, A Students Teaching Students Course, Megan M. Nepshinsky, Alexa Kretsch, Benjamin Negrete

Senior Honors Projects

This class is the second project under the new Honors Program initiative, Students Teaching Students (STS). STS was brought to URI and implemented by Bridget Griffith as part of her Senior Honors Project in 2011. Students apply in their Junior year to design, research, and teach an honors course on a topic about which they are passionate. The STS program allows motivated honors students to have a unique opportunity to lead a class as student professors. This allows students a freedom of creativity on the topic, assignments, and teaching style. Drawing on our own experiences, we were able to create …

2011 Annual Report, Various Authors

Annual Reports

No abstract provided.

Genetic Variation In Potentially Virulent Vibrio Parahaemolyticus From The Northern Gulf Of Mexico, Nicholas Felix Noriea Iii

Dissertations

Vibrio parahaemolyticus (V_p) is a gram-negative bacterium found naturally in marine and estuarine environments. V_p is found in oysters including those which are later consumed by the public. Sub-populations of potentially virulent Vp contain specific virulence factors and are relevant human pathogens capable of causing gastroenteritis, wound infection, and death. The tdh and trh genes, both encoding hemolysins, have been correlated with the majority of clinical V_p isolates but have not been shown to be the definitive virulence factors.

A total of 146 V_p isolates from the northern Gulf of Mexico were collected and probed …

Effects Of Hypoxia And 4-Tert-Octylphenol On Gene Expression Profiles Of The Sheepshead Minnow (Cyprinodon Variegatus), Arthur Alan Karels

Dissertations

Hypoxia occurs in estuaries of northern Gulf of Mexico and world-wide, with increasing frequency/severity via eutrophication and anthropogenic influences. Hypoxia inducible factors (HIFs) form transcriptional complex and bind DNA at hypoxia responsive elements (HREs) in promoter regions of genes needed for systemic and cellular adaptation of fish to low dissolved oxygen (hypoxia, DO <2.0 mg/ml). Hypoxia-induced activation of HIF-αs can lead to a cascade of downstream activation, such as erythropoietin (EPO). Return to normal DO levels (normoxia), prolyl hydroxylases (PHDs) are activated to degrade HIF-αs back to baseline. Fish are affected by environmental estrogen mimics, like 4-tert-octylphenol (4tOP), binding estrogen receptor alpha (ERα) at estrogen responsive elements (EREs) and activating genes vitellogenin (VTG). Previous research showed overlap or crosstalk between these two mechanistic pathways. Hypoxia triggers unknown factors regulating ERE-mediated ERα signaling pathway, and stressor combinations could increase/decrease hypoxic or endocrine pathway. Research examined molecular/physiological effects of hypoxia (acute and chronic, moderate and severe) and 4tOP (~60μg/L)on adult male and/or female sheepshead minnow (Cyprinodon variegatus). Three genes identified, cloned, and sequenced (HIF-1α, HIF-2α, and PHD3), plus previously identified genes EPO and VTG, were examined in liver/testes exposed to hypoxia and/or 4tOP for cellular/physiological changes. Endpoints examined included mRNA expression from real-time PCR of HIF-1α, HIF-2α, PHD3, EPO, and VTG using cDNA from total RNA extracts, and microarray analyses of genes expressed during the transition from hypoxia back to normoxia. Phylogenetic analyses confirmed isolation of two HIF-α isofoms (HIF-1α and HIF-2α) and the PHD3 isoform. Significant up-regulation of PHD3 occurred within 10 hrs of chronic hypoxia, and persisted when severe (1.5 mg/L) and declined when moderate (~2.5mg/L). Significant up-regulation of HIF-1α and EPO occurred within 30 minutes to 2 hours of onset of acute severe and very severe (~1.08mg/L) hypoxia. Hypoxia acted similar to an estrogen mimic, with huge up-regulation of VTG gene expression in males, and increased VTG levels (additive effect) when hypoxia was combined with 4tOP. Microarray analyses showed 125 genes with significant transcriptional change, with up- or down-regulation from transitions of: (1) hypoxia (72 hrs) to normoxia (74 hrs) and (2) hypoxia+4tOP (72 hrs) to normoxia+4tOP (74 hrs).

Comparison Of Creel Survey Data To Traditional Sampling Techniques In Pit-Lake Fisheries Of Muhlenberg County, Kentucky, Derek L. Rupert

Masters Theses & Specialist Projects

Populations of largemouth bass, Micropterus salmoides, and bluegill, Lepomis macrochirus, were evaluated from five pit-lakes in Muhlenberg County, Kentucky, to determine if accurate proportional stock density (PSD) data can be obtained from a mandatory creel survey. It was hypothesized that the proportion of stock-to-quality (300-400mm) and quality (+400mm) largemouth bass from four years (2007-2010) of creel survey data would be statistically similar to those generated through on-site sampling in 2011. Fish were collected via a combination of gill netting, seining, hook-and-line fishing, and boat-mounted electro-fishing. In two of the pit-lakes, the sampling-generated length frequency data was not significantly …

Host Specificity Of Ecuadorian Bat Flies (Diptera: Streblidae), Eric Stamper

Mahurin Honors College Capstone Experience/Thesis Projects

Diptera: Streblidae are ectoparasites of bat populations found in many locations throughout the world. These ectoparasites are generally known as bat flies. They attach themselves to the wing membranes and bodies of bats to bite and feed on their blood. Using a large sample consisting of over 2,000 bats and 6,000 bat flies, I have conducted a study of the degree of host specificity of these ectoparasites. Host specificity is a measurement of the degree to which a particular parasite is restricted to its host or hosts. Here I find evidence to support more recent findings that bat flies are …

Kentucky Warbler (Vol. 88, No. 2), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Blood Cortisol Concentrations Predict Boldness In Juvenile Mulloway (Argyosomus Japonicus), Vincent Raoult, Culum Brown, Amina Zuberi, Jane E. Williamson

Sentience Collection

There is a growing interest in animal personality because individual variation is the substrate of the evolutionary process. Despite revelations that personality traits affect key fitness variables, little is known about the proximate mechanisms generating consistent behavioural differences between individuals. Boldness, the propensity to take risks, is one of the most widely studied aspects of personality in fishes. We assessed the position of juvenile Argyosomus japonicus on the ‘‘boldness–shyness’’ continuum by repeatedly recording the time taken to exit a refuge and explore a novel environment. Stress-related hormone concentrations after exposure to a mild stressor were analysed 1 month before behavioural …