Digital Commons Network^™

Desperate Larva Or Death Before Dishonor: Can Old Coral Larvae Replenish Degraded Reefs?, Nepsis García Lara

All HCAS Student Capstones, Theses, and Dissertations

The recovery of coral populations depends largely on larval recruitment. Coral larvae settle in response to environmental cues that indicate habitat quality. Newly competent larvae typically avoid settling on substrates with high macroalgal cover and sedimentation, and thus might never recruit to degraded reefs, hindering their recovery. What is unknown is if settlement preferences change as larvae age. In the absence of suitable settlement cues, lecithotrophic larvae delay settlement and might do this until either dying (Death Before Dishonor Hypothesis) or becoming less discriminatory and settling regardless of their specific habitat requirements (Desperate Larva Hypothesis). To test these hypotheses in …

Ten Tips Taylored For Successful Talks With Landowners In Ranching And Range Management Landscapes, Taylor J. Linder, Susan N. Ellis-Felege, Christopher J. Felege

Biology Faculty Publications

Privately owned lands offer substantial land area that have the potential to contribute greatly to wildlife conservation. However, it is dubious for private landowners to engage in such programs that have not readily accepted their input. Given the future directions of wildlife conservation and needing to heavily integrate private lands into habitat objectives to meet wildlife population standards, it will be of growing importance to consider the needs and wants of private landowners to meet production goals and simultaneously wildlife habitat goals (and not the other way around). We recognize the important methodological research that has built foundations for current …

The Effect Of Water Flow Rates On The Survival And Growth Rates Of Three Caribbean Bouldering Coral Species Juveniles In An Indoor Versus Outdoor Environment, Ian Michael Johnson

All HCAS Student Capstones, Theses, and Dissertations

Coral reefs are vital ecosystems for the world’s oceans and humanity; however, they are threatened by climate change, disease, and local anthropogenic stressors, and need assistance to recover. Traditional reef restoration efforts (fragmentation and outplanting are helping but are limited in effectiveness by not increasing genetic diversity. Ex situ sexual propagation for corals provides new, genetically different coral recruits. However, this process is laborious, expensive and time consuming, especially at the scale required to effectively contribute to the widespread recovery. To lower costs, two key parameters that require optimization to hasten the growth of coral recruits are water flow and …

Evaluation Of The Virulence Potential Of Avian Pathogenic Escherichia Coli Isolated From Broiler Breeders With Colibacillosis In Mississippi, Jiddu Joseph

Theses and Dissertations

Avian pathogenic Escherichia coli (APEC) is a bacterium that is responsible for colibacillosis in birds. However, information about broiler breeder APEC isolates is limited, but the data is critical due to the transfer of this bacteria down the production pyramid to progenies resulting in high mortality. Therefore, we evaluated the phenotypic virulence characteristics of 28 isolates using embryo lethality and day-old chick challenge assays. Also, the in vitro adhesion and invasion potential of selected nine isolates were identified. Results showed more than 1/3^rd of the isolates were highly virulent and the virulence increased as the number of virulence-associated genes …

Evaluation Of Heat Abatement Use, Vaginal Temperature, And Activity Of Pregnant, Lactating Holstein Cows Housed On Pasture With Or Without The Choice Between Shade And Sprinklers For Heat Abatement., Kevin M. Braman

Theses and Dissertations

The objective of this thesis was to determine how pregnant, lactating Holstein cows on pasture interact with shade and sprinklers when offered a choice or no-choice between methods. Authors hypothesized that cows would use shade more then sprinklers, but sprinklers would be more effective at cooling cows. Additionally, authors predicted cows using shade more would have decreased lying bouts and steps and an increase in time lying, compared to cows choosing sprinkler. Results indicate cows will use both methods when offered alone or together. However, cows will choose to be exposed or in shade at lower temperature humidity index values, …

A Survey Analysis Of Southeastern U.S. Dairy Producers’ Emotional States And Their Subjective Pain Perception Of Dairy Cows, Michelle M. Schuh

Theses and Dissertations

Dairy producers play a central role in evaluating and seeking treatment or care for animal pain. The primary aim of this study is to examine dairy producers’ emotional states and professional quality of life and analyze the relationship between these variables and their perception of pain in dairy cattle. Dairy farm owners and managers of 65 southeastern U.S. herds participated in a survey that included demographic information, the Depression, Anxiety, and Stress Scale, a modified Professional Quality of Life Scale, and 23 items requiring participants to evaluate various painful conditions in cattle.

Evaluating The Role Of The Bovine Vaginal Microbiome In Neonatal And Maternal Health Outcomes, Riley D. Messman

Theses and Dissertations

The dam vaginal microbiota is the first major microbial inoculating community within the neonate. The composition of the dam vaginal microbiota has implications in calf commensal microbiota development. Alterations of the dam microbial community prior to parturition could alter inoculating communities and immune responses in both the dam and calf. Thus, authors aimed to elucidate the microbial community composition of the bovine dam vaginal and calf nasal microbiota post-partum after utilizing betadine lavages (BL). The dam vaginal and calf nasal microbial communities and immune responses were evaluated at 0-, 15-, 30- and 60-day post-partum. Microbiota composition of the dam haircoat, …

Non-Destructive Evaluation Of White Striping And Microbial Spoilage Of Broiler Breast Meat Using Structured-Illumination Reflectance Imaging, Ebenezer O. Olaniyi

Theses and Dissertations

Manual inspection is a prevailing practice for quality assessment of poultry meat, but it is labor-intensive, tedious, and subjective. This thesis aims to assess the efficacy of an emerging structured illumination reflectance imaging (SIRI) technique with machine learning approaches for assessing WS and microbial spoilage in broiler breast meat. Broiler breast meat samples were imaged by an in house-assembled SIRI platform under sinusoidal illumination. In first experiment, handcrafted texture features were extracted from direct component (DC, corresponding to conventional uniform illumination) and amplitude component (AC, unique to the use of sinusoidal illumination) images retrieved from raw SIRI pattern images build …

Oocytes To Offspring: Optimizing Assisted Reproductive Technologies (Art) To Support Amphibian Conservation, Shaina Shaira Lampert

Theses and Dissertations

The increasing dependence on amphibian captive breeding programs to support at-risk populations has led to growth in the application of assisted reproductive technologies (ART) to aid with the management of decreasing population density and genetic diversity. Already the integration of ART within these programs has led to monumental successes and shows great promise as a means to augment amphibian breeding efforts when natural breeding has low success. The focus of this project was to continue the optimization of ART to aid in making these technologies more widely applicable. Specific objectives include developing a framework that can be used to standardize …

Nutritional Strategies To Reduce Salmonella Enteritidis Infections In Laying Hens: Prebiotic, Probiotic, And Precision Biotics, Ishab Poudel

Theses and Dissertations

Salmonella Enteritidis (SE) infections in laying hens is a significant cause of foodborne illness linked to the consumption of contaminated eggs. This dissertation encompasses novel strategies aimed to explore appropriate in-vitro and in-vivo models to evaluate the effects of these novel strategies to reduce SE in laying hens. Firstly, we investigated the potential probiotics, prebiotics, and essential oil combinations to reduce SE in-vitro. The results showed that probiotics and prebiotics are equally effective to reduce SE. In subsequent studies, we evaluated the role of vaccination history on cecal microbiota and SE infections with various doses of oral SE challenge. …

Use Of Comparative Genomics And In Vitro Screening Approach To Identify Vaccine Candidates For The Food-Borne Pathogen Campylobacter Jejuni, Sabin Poudel

Theses and Dissertations

Campylobacteriosis is a leading foodborne illness worldwide, primarily caused by Campylobacter jejuni (C. jejuni) which is associated with poultry consumption. The emergence of antibiotic resistance has emphasized the need for alternative strategies to control C. jejuni colonization in poultry. To assess the prevalence of C. jejuni in poultry, 270 cloacal swab samples were collected from broilers raised under No-Antibiotics Ever system. Among these samples, 16.3% were identified as C. jejuni positive. Notably, these isolates exhibited a diverse range of virulence factors and antimicrobial resistance genes, with 61.36% of isolates showing hyper-motile and 20.45% demonstrating multidrug resistance. Following isolation, whole genome …

Effects Of Fire Seasonality On Bachman’S Sparrows In The Longleaf Pine Forests Of Southern Mississippi, Michael D. Warren

Theses and Dissertations

The Bachman’s sparrow (Peucaea aestivalis) is a ground-dwelling, pine-obligate species experiencing range-wide population declines due to land development and fire exclusion. We explored the effects of fire seasonality on wintering Bachman’s sparrow abundance in Southern Mississippi from 2021-2022. We used generalized linear mixed models to investigate differences in sparrow abundance and vegetation characteristics following dormant and growing season fire. We explored the effects of growing season fire on breeding territory vegetation characteristics. Our results indicate that burn type (dormant vs. growing season), native grass groundcover, and shrubs over 1m were the most significant predictors for wintering Bachman’s sparrow …

How Can Windbreaks Protect Livestock From The Cold?

Working Trees (USDA-NAC)

Windbreaks can protect livestock from the cold resulting in improved feeding efficiency, increased milk production, improved overall animal health, increased forage yield, and other benefits.

Mammalian & Avian Community Response To African Elephant (Loxodonta Africana) Habitat Modification In Southeastern Kenya, Dakota Vaccaro

Masters Theses & Specialist Projects

African elephants (Loxodonta africana) play a significant role in the modification of their habitat, foremost by decreasing woody vegetation cover and density and in some parts of Africa high elephant densities damage small sanctuaries and preserves. For wildlife that depend on forested patches, this increased modification could lead to displacement and/or decreases in abundance. If suitable habitat is not available elsewhere, elephants and other wildlife may move closer to humans in search of resources, which can result in increased human-wildlife conflict. Evaluating this impact was one of the goals of the Elephants and Sustainable Agriculture in Kenya (ESAK) …

Microstructural Characterization Of High-Protein Dairy Powders, Prafulla Salunke, Athira Syamala, Lloyd Metzger

Dairy Science and Food Science Faculty Publications: 2022 to Present

Dairy proteins are potential and multipurpose ingredients used to prepare various food products. It exhibits many beneficial functionalities and bioactivities in the processing of food products. All the functionalities of different dairy proteins depend on their peculiar structural characteristics. So, the present study aimed to characterize high-protein powders with different matrices (milk protein concentrate (MPC), rennet casein (RCN), micellar casein concentrate (MCC), whey protein isolate (WPI), and native whey concentrate (NWC)) for their composition, handling, and reconstitution properties, and microstructure and compared them with whole milk powder (WMP) and skim milk powder (SMP). The bulk density of the high-protein powders …

Song Structure Of Male Northern House Wrens And Patterns Of Song Production And Delivery Across The Nesting Cycle, Rachael A. Disciullo, Scott K. Sakaluk, Charles Thompson

Faculty Publications – Biological Sciences

Studies of the role of bird song in avian communication have revealed numerous functions across many species, including its critical importance in female mate choice (intersexual selection) and male-male competition (intrasexual selection). A first step in understanding the role of song in different contexts is to describe the note, syllable, and song-types that occur, as well as their patterns of production within a population. Here, we do so for a north-central Illinois, USA, population of Northern House Wrens (Troglodytes aedon). As has been described in other populations, most songs (≈ 1–3 s in duration) contain an introductory section of soft, …

Effects Of Antagomir-22-3p On Muscle Size In Older Lambs Using Computed Tomography Imaging, Anna Brewer

All Theses

Background

Texel sheep are known for their double-muscling phenotype and extraordinary meat production (Miar et al., 2014). Satellite cells are essential for this desired muscle growth. MicroRNAs can silence target genes by binding to the target messenger RNA (mRNA) (Horak et alk., 2016). Antagomirs are miRNA inhibitors and are used for loss of function studies. MiR-22-3p is known for inhibiting skeletal muscle growth and directly targets HDAC4, TGFBR1, PAX7 mRNA expression. Computed Tomography (CT) imaging is now growing in popularity in the animal science field to record changes in muscle characteristics.

Materials and Methods

Texel x Suffolk lambs (n=4) were …

Dietary Histidine: Broiler Responsiveness With And Without Endogenous Histidine Reserve Consideration, Kenneth Blake Nelson

Graduate Theses and Dissertations

In the past, histidine warranted little concern from poultry nutritionists because it was seldom deficient in practical broiler diets. With most essential amino acids now commercially available as feed-grade forms to partially replace intact protein ingredients, histidine and its minimum in least-cost formulation are receiving fresh attention. Published histidine requirement data for broilers are sparce, and many of these studies have been conducted on males within the first three weeks post-hatch. Moreover, rudimentary dose-response experimental designs may not be applicable to estimate histidine requirements in later growth periods because histidine is uniquely stored within the body. In times of dietary …

Recreational Fishing For Western Rock Lobster: Estimates Of Participation, Effort And Catch In 2022/23, Claire B. Smallwood, Karina L. Ryan, Alissa C. Tate, Lucy J. Rudd

Fisheries Research Reports

The Western Rock Lobster (WRL) (Panulirus cygnus) is the most commonly caught Rock Lobster (RL) species in Western Australia. Catches from the commercial and recreational sectors are required to determine and monitor Total Allowable Commercial Catch (TACC), Total Allowable Recreational Catch (TARC) and proportion of Allowable Harvest Level (AHL) achieved. The TARC is set at 5% of the AHL and evaluated in the harvest strategy based on a 5-year average. Data for the recreational sector are obtained using phone-recall surveys to provide estimates of participation, fishing effort and retained catch (by numbers) from recreational fishers who hold a RL licence. …

New Species Of Dryolestoid From The Late Cretaceous Allen Formation And Implications For South American Faunal Diversity., Brigid Erin Connelly

Electronic Theses and Dissertations

Dryolestoids are extinct cladotherians mammals from the Jurassic and Cretaceous periods. I describe a collection of dryolestoid specimens from the Late Cretaceous localities of Cerro Tortuga (Allen Formation), Anfiteatro 1, and Shining (both La Colonia Formation) from Patagonia, Argentina. Using comparative morphology, I identify a new species of meridiolestidan dryolestoid based on eleven specimens across both formations. The new species’ recovery from La Colonia Formation represents the first dryolestoid connection between the two approximately contemporaneous formations. The species’ morphology may represent an ecological shift within Meridiolestida from insectivory to herbivory, showing a transition in characters between the plesiomorphic sharp-toothed meridiolestidans …

Western Pearlshell (Margaritifera Falcata) Extirpation In The Smith River, Montana, With A Possible Link To Warming Water Temperatures, David M. Stagliano

Western North American Naturalist

Western pearlshell (Margaritifera falcata) populations are in decline across the species’ range in western North America. In 2008, Montana categorized the western pearlshell as an imperiled species of concern (S2) based on reduced detections at historically occupied sites and few viable populations. Between 2004 and 2014, we surveyed 24 Smith River reaches (61 total sites in the watershed) for the presence of western pearlshell populations. Despite historical and anecdotal descriptions of abundant western pearlshell in the Smith River, intensive structured surveys between 2004 and 2007 documented only 4 sections with low-density, nonviable western pearlshell populations (<10 individuals, large size classes [average total length = 70 mm], with no signs of recruitment). In 2014, we revisited these occupied sites to repeat surveys (expending significantly more search time) and observed no live individuals. This was surprising given that, although those populations would certainly die out without recruitment, older individuals can oftentimes persist for decades. Therefore, we consider the western pearlshell mussel extirpated from the Smith River watershed. We reviewed long-term USGS gage discharge and temperature data near the western pearlshell populations to evaluate the potential causal factors for, and timing of, the extirpation. From 3 July to 3 August 2007, water temperatures were at or above the thermal stress levels of the western pearlshell (>25 °C, …

Informing Management Strategy For The Relict Leopard Frog (Rana Onca): Insights Into Breeding Biology And An Attempt To Improve Augmentation Success Through Pre-Exposure And Clearance Of An Emerging Amphibian Pathogen, Rebeca Rivera

UNLV Theses, Dissertations, Professional Papers, and Capstones

The Relict Leopard Frog, Rana onca, is a species of conservation concern that suffered a decline sometime during the 20th century. Even after two decades of intensive management, the species currently only occupies 20 spring sites in southern Nevada and northwestern Arizona. The causes for the historical decline are mostly speculative, but relate to habitat loss, introduced predators, and emergent disease. Since 2001, R. onca has been under an intensive conservationprogram managed by a multiagency conservation team. There are several objectives specified in the program including the need to investigate the biology of the species and incorporate findings into …

Developing A Predictive Geospatial Habitat Model For A Rare Species Of Salamander In Tennessee: A Case Study For The Green Salamander (Aneides Aeneus), Erin Gaylord

Masters Theses and Doctoral Dissertations

Green Salamanders (Aneides aeneus; Cope and Packard 1881) are a secretive and cryptic species of salamander that can be relatively difficult to detect, due to their occupation of arboreal habitats. The incorporation of geospatial tools is critical in developing models that can be used to predict undocumented locations in Tennessee and elsewhere. Locating the species in Tennessee was accomplished through a total of 18 localities and with that, several habitat characteristics were able to be identified including, but not limited to: appropriately shaded and structured rocks, typical fauna co-occurring within the area, and general topography of the area. Of the …

Leveraging Eco-Evolutionary Models For Gene Drive Risk Assessment, Matthew A. Combs, Andrew J. Golnar, Justin M. Overcash, Alun L. Lloyd, Keith R. Hayes, David A. O'Brochta, Kim M. Pepin

United States Department of Agriculture Wildlife Services: Staff Publications

Engineered gene drives create potential for both widespread benefits and irreversible harms to ecosystems. CRISPR-based systems of allelic conversion have rapidly accelerated gene drive research across diverse taxa, putting field trials and their necessary risk assessments on the horizon. Dynamic processbased models provide flexible quantitative platforms to predict gene drive outcomes in the context of system-specific ecological and evolutionary features. Here, we synthesize gene drive dynamic modeling studies to highlight research trends, knowledge gaps, and emergent principles, organized around their genetic, demographic, spatial, environmental, and implementation features. We identify the phenomena that most significantly influence model predictions, discuss limitations of …

Genetic Parameters For Heat And Salinity Stress Tolerance In Northern Gulf Of Mexico Eastern Oyster, Crassostrea Virginica, Paul Mcdonald

Master's Theses

Summer mortalities in Gulf of Mexico eastern oysters have been linked to excessively high temperatures, and chronic exposure to low salinity. In this study, the feasibility of improving tolerance to these two stresses by selection was evaluated in common garden experiments employing a pool of 160 families. Heat stress tolerance was evaluated in seed (3.5 months) and adults (14 months) by applying a sustained temperature of 38-39 °C until all oysters expired. Low salinity challenges on juveniles (9 months) and adults (15 months) applied a salinity of 2.5 psu. Survival duration of individual oysters during challenges was recorded and tissue …

Consistency Is Key: Investigating Vocal Consistency In Field Sparrows, Stephanie Stanton

Masters Theses

No abstract provided.

The Effects Of Caffeine On Activity Levels And Motor Behaviors In The Honeybee, Apis Mellifera, Johana Castro

Theses and Dissertations

Caffeine is produced by plants as a defense mechanism against herbivores and pathogens. However, caffeine was found in floral nectar, which contradicts nectar's role in attracting pollinators. Interestingly, low concentrations of caffeine were shown to increase alertness and locomotion in mammals and invertebrates, indicating it may enhance the productivity of pollinators. This investigation examined how caffeine affects activity levels and motor behaviors in the honey bee, Apis mellifera. To test overall activity, forager bees were collected and fed 1 M sucrose to satiation. The following day, bees received 10 µl of 1.0 M sucrose alone or with caffeine ranging from …

Evaluation Of Bone And Muscle Quality In Laying Hens Using Quantitative, Radiographic, Computed Tomographic, Biomechanical, And Tissue Level Measures, Cerano Harrison

All Dissertations

Bone quality is an important measure of welfare in laying hens for researchers, veterinarians, and commercial producers who bear the responsibility of providing them with care. Breeding practices that have resulted in high egg productivity of laying hen strains have resulted in an increased susceptibility to developing osteoporosis. Osteoporosis, a decrease in mineralized bone, is accompanied by increased bone fragility that can lead to potentially painful bone fractures that can inhibit some aspects of the birds natural living, respiration and flight being two examples. Current methods of examining laying hen bone quality involve the implementation of diagnostic imaging and biomechanical …

Effect Of Authentic Kefir And Nigella Sativa On Broilers Challenged By Coccidia And Clostridium Perfringens, Julian E. Nixon

All Dissertations

Black seed oil concentrations of 0%, 0.1%, 1% and 5% were added to milk inoculated with kefir grains and incubated at 25°C for 22 h. The pH and microbial count indicated 1% black seed oil caused low inhibition (P > 0.05) of fermentation, but 5% black seed oil caused significant inhibition of the kefir microorganisms (P < 0.05).

Cobb 500 male chicks (n = 256) were distributed in a randomized block design and received one of four treatments: CTRL1 (Non-medicated, no kefir, no Clostridium perfringens), CTRL2 (Non-medicated, no kefir, C. perfringens inoculated), CTRL3 (BMD medicated, no kefir, C. perfringens inoculated), KTRT (Non-medicated, …

Consumption Of A Western Diet Enhanced Colitis-Associated Colorectal Cancer And Dysbiosis Of The Fecal Microbiome In Mice Notwithstanding Dietary Intervention Or Fecal Microbiome Transfer, Daphne Michelle Rodriguez Jimenez

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In a rodent model of inflammation-associated colorectal cancer, consumption of a Western-style diet increases gut inflammation and enhances risk of developing colon tumors. The goal of this dissertation was to understand the contribution of bacteria within the large intestine on colon inflammation and colon tumorigenesis. Two pre-clinical animal studies were performed using two different intervention strategies to shift the microbiome, and potentially gut inflammation and tumor development: 1) an experiment using dietary supplementation with black raspberries, a functional food enriched in bioactive anthocyanins with purported antiinflammatory activity, and 2) an experiment using fecal microbiota transfer from mice fed a healthy …