Digital Commons Network^™

Role Of The Placenta In Developmental Programming: Observations From Models Using Large Animals, L. P. Reynolds, C. R. Dahlen, A. K. Ward, M. S. Crouse, P. P. Borowicz, B. J. Davila-Ruiz, C. Kanjanaruch, K. A. Bochantin, K. J. Mclean, K. L. Mccarthy, A. C.B. Menezes, W. J.S. Diniz, R. A. Cushman, J. S. Caton

Department of Animal Science: Faculty Publications

Developmental programming, which proposes that “insults” or “stressors” during intrauterine or postnatal development can have not only immediate but also long-term consequences for healthy and productivity, has emerged as a major biological principle, and based on studies in many animal species also seems to be a universal phenomenon. In eutherians, the placenta appears to be programmed during its development, which has consequences for fetal growth and development throughout pregnancy, and likewise has long-term consequences for postnatal development, leading to programming of organ function of the offspring even into adulthood. This review summarizes our current understanding of the placenta’s role in …

Freshwater And Saline Flood Plain Wetlands Of The North Platte River - Sources And Seasonal Dynamics: Kiowa Wildlife Management Area, Morrill, Ne, Anne M. Matherne

Nebraska Game and Parks Commission: White Papers, Conference Presentations, and Manuscripts

The Kiowa Wildlife Management Area (WMA), a 1 mi² wetland and upland on the North Platte River, was acquired by the Nebraska Game and Parks Commission (NGPC) in 1993. The WMA comprises two distinct wetland types, alkaline and freshwater, both of which provide important wildlife habitat. It was the goal of this project to understand the hydrology controlling the development and maintenance of these wetlands in order to anticipate the affect of management strategies on the wetland complex. The study was designed to develop a comprehensive picture of the hydrogeology of both the fresh and alkaline portions of the WMA, …

Willa Cather Ecological Prairie: An Ecological Overview, Harold G. Nagel

Nebraska Game and Parks Commission: White Papers, Conference Presentations, and Manuscripts

This report includes data gathered on the ecology of a 610 acre mixed grass prairie in south-central Nebraska over a two year period.

An attempt was made to identify as many of the plant and animal species present and to determine density and standing crop biomass wherever possible, under the time and financial constraints present. Few attempts have been made to develop complete species checklists for both animals and plants in prairies. The data collected are considered baseline conditions against which comparisons can be made after a management plan is carried out.

This study of Cather Prairie had several objectives. …

The Flora Of The Oak-Wooded Drainages At The Timber Point Area, Southeastern Butler County, Nebraska, Steven B. Rolfsmeier

Nebraska Game and Parks Commission: White Papers, Conference Presentations, and Manuscripts

The Timber Point Area is a 160-acre recreational/wildlife area occupying to S½ of the SW¼ sec. 22 and the adjacent N½ of the NW¼ sec. 27, T14N R4E. The area is located in the loess-mantled glacial-till hills of southeast Nebraska not far from the terminal moraine of the Pre-Illinoian glacial sheet, one mile south and two miles east of the present town of Brainard. The uplands at Timber Point were apparently in pasture prior to purchase by the NRD, with some cultivated fields occupying the lower ground along a stream valley which southwest to northeast through the property. This small …

The Flora Of Two Oak Drainages At The Red Cedar Area, Southwestern Saunders County, Nebraska, Steven B. Rolfsmeier

Nebraska Game and Parks Commission: White Papers, Conference Presentations, and Manuscripts

The Red Cedar Area is a 175 acre recreational/wildlife area occupying the SE sec. 20, T14N R5E and a small portion of sec. 29 directly to the south. The area is located in the glacial-till hills of southeast Nebraska ca 5 mi. north and 2 mi. west of Valpraiso. The bulk of the area was cultivated farmland prior to purchase by the NRD, and nearly all upland areas here were cultivated cropland, save for some steep oak-wooded drainages on the east side of Hunter’s Slough, which ran northwest to southeast through the property. The creek was dammed after NRD purchase …

The Vascular Plants Of The Woodlands Of South Table Creek In The Vicinity Of The National Arbor Day Center, Nebraska City, Nebraska, Steven B. Rolfsmeier

Nebraska Game and Parks Commission: White Papers, Conference Presentations, and Manuscripts

The objective of this study is to describe the composition of the vascular plant flora of a three-quarter mile stretch of woodland along South Table Creek managed by the National Arbor Day Foundation in Nebraska City. This 50-60 acre woodland is representative of the many small tributaries of the Missouri River in southeastern Nebraska, and may represent the only natural “preserve” of native forest on the west side of the Missouri River between Fontenelle Forest and Indian Cave State Park. All naturally-occurring species encountered in this study are listed alphabetically by family at the end of this paper, and a …

The Flora Of Meadowlark Prairie, Northeastern Seward County, Nebraska, Steven B. Rolfsmeier

Nebraska Game and Parks Commission: White Papers, Conference Presentations, and Manuscripts

This report is on the flora of the Meadowlark Prairie. The prairie is an approximately 7 acre remnant of virgin tall-grass prairie in extreme northeastern Seward County, Nebraska. The remnant is triangular in shape and lies on a gentle southeast-facing slope with a total relief of 50 ft., along the east boundary of the Meadowlark Recreation Area. The prairie is surrounded on the east side by a small brome-infested prairie remnant and cultivated field off the NRD property, and on the remaining sides by formerly cultivated fields now seeded to native warm-season grasses. Soils in the area are primarily derived …

The Flora And Plant Communities Of The Ceresco Saline Basins, Southern Saunders County, Nebraska, Steven B. Rolfsmeier

Nebraska Game and Parks Commission: White Papers, Conference Presentations, and Manuscripts

This report is on the flora and plant communities of the Ceresco Saline Basins. The basins are along Rock Creek east of Ceresco and represent the northernmost representatives of the “eastern saline marsh” community in Nebraska. Eastern saline marshland is mostly restricted to the Salt Creek drainage in northern and central Lancaster County, though the Ceresco basins extend a mile or more north into Saunders County in the floodplain of Rock Creek. Some of the wetland in this area is currently under management of the Nebraska Game and Parks Commission, while some of the northernmost portions of the basins are …

Go Big Or Go Home: Divergence In Morphology And Behavior Between Island And Islet Populations Of The San Cristóbal Lava Lizard (Microlophus Bivittatus), Emilio J. Mancero

Masters Theses

Rapid divergence in body size is a well-documented phenomenon among island species, associated with macroevolutionary processes of diversification and adaptive radiation. The effect of insularity in determining larger body size as an optimal phenotype is primarily attributed to lower predation and greater intraspecific competition on islands. Our study examined interpopulation variation in morphology, social behavior and predator avoidance in Galápagos lava lizards endemic to San Cristóbal island (Microlophus bivittatus), and the nearby islet Isla Lobos. Islet populations have fewer competitors than those on the island, where native and introduced predators are abundant. We simulated interactions with predators and …

Who Is The Good Boy/Girl? Perspectives Of French Handlers In Aai On The Selection Of Their Dogs, Alice Mignot, Gérard Leboucher, Véronique J. Servais, Karelle De Luca

People and Animals: The International Journal of Research and Practice

Animal-assisted interventions (AAI) are well implemented in various health care settings; however, there is little data on the characteristics of the mediation dogs and their selection, which can influence the well-being of both the dogs and the beneficiaries. This study aims to gain a better understanding of the characteristics of French mediation dogs and the context in which they are selected to guide future research working on behavioral criteria for mediation dogs and help provide a basis for better selection of dogs in the field. To this end, we interviewed 111 French handlers in AAI, who work with at least …

Gibberellic Acid Data, Amber Johnson

ScholarsArchive Data

This dataset contains plant emergence and survival data collected from a field study which focused on overcoming seed dormancy. Seed dormancy can present a challenge within a restoration scenario where rapid establishment is required. Soaking seeds in gibberellic acid (GA₃) can overcome dormancy and increase germination but this treatment may not be effective outside the laboratory. An easier and potentially more effective method to apply this hormone is to coat seeds with a GA₃-impregnated polymer, which provides a slow release of GA₃. Seed dormancy can also be mitigated by creating a favorable microsite with …

Evaluation Of Tissue Depth, Captive Bolt Penetration Force And Energy, And Potential For Bolt-Thalamus Contact In Cadaver Heads From Physically Castrated Market Barrows And Immunocastrated Boars, Emma M. Hamilton, Karly N. Anderson, Ashlynn A. Kirk, Katherine D. Bishop, Joseph Joseph Shakal, Kurt D. Vogel

Department of Animal Science: Faculty Publications

The main objective of this study was to describe tissue thicknesses of cadaver heads from physically castrated market barrows (PC MARKET BARROWS) and immunocastrated boars (IC BOARS) at the frontal penetrating captive bolt (PCB) placement. Other objectives were to describe differences in bolt force and energy requirements to penetrate and describe potential for bolt-thalamus contact. Forty-four heads were obtained from PC MARKET BARROWS (n = 22) and IC BOARS (n = 22) of similar age and size that were rendered insensible with CO₂. Mean HCW was 117.32 ± 3.52 kg. Snout to poll distance (cm) and …

Observations Of The Association By Early-Juvenile Western Rock Lobster Panulirus Cygnus George, 1962 With Seagrass Assemblages (Decapoda: Achelata: Palinuridae), Daphne Oh, Tim J. Langlois, Michael A. Brooker, Hugo Salinas, Jason R. How, Simon De Lestang

Fisheries Research Articles

The fishery of the western rock lobster, Panulirus cygnus George, 1962, is Australia’s most valuable wild-caught single-species fishery. Recruitment in some regions of the fishery was observed to be significantly lower than expected after the 2010/2011 West Australian marine heatwave that caused extensive disturbance of dominant coastal habitats. This event generated interest in the study of the factors influencing survival and recruitment of post-larval benthic P. cygnus after settlement. The habitat associations of the highly cryptic post-settlement early-juveniles were previously unknown, with only anecdotal observations of individuals within limestone crevices in nearshore habitats. Our study used early-juveniles derived from …

Utility Of Body And Otolith Morphometry To Discriminate Cryptic Juveniles Of Two Sympatric Red Snappers (Perciformes: Lutjanidae), Miwa Takahashi, Corey B. Wakefield, Stephen J. Newman, Kyle B. Hillcoat, Benjamin J. Saunders, Euan S. Harvey

Fisheries Research Articles

The sympatric red snappers, Lutjanus erythropterus and Lutjanus malabaricus, are highly valued by commercial and recreational fishers along the tropical northern coasts of Australia and throughout their distribution. Studies on the life history and ecology of these congeners are confounded by difficulties in distinguishing the cryptic juveniles of each species (i.e., < 200 mm total length). This study aimed to validate a robust and cost-effective method to discriminate these juveniles using body and/or otolith morphometric data in a multivariate analysis. Juvenile samples were collected from the northwest (n = 71) and northeast (n = 19) coasts of Australia, and species identification was confirmed using DNA barcoding. The most parsimonious multivariate models achieved accurate species prediction rates of 98.8%, which consisted of just three body variables (dorsal fin length, the distance from the snout to the anterior edge of the eye, and either jaw length or distance from the snout to the preoperculum). The high level of discrimination for these cryptic juveniles highlights the robustness of this morphometric approach. The slightly lower rate of discrimination using otolith morphology (84.9%) was associated with greater regional variation in L. malabaricus between the northwest and northeast coasts. Slight variations in otolith shape are typically used to determine stock structure, which highlights the potential need to collect samples over a broader area of a species geographic range when using an otolith morphometric discrimination model. The method outlined in this study could be applied to distinguish other cryptic congeneric fish species, including from archived otolith collections. Moreover, this method has the potential to be utilized in assessing species compositions using body measurements from in situ stereo-video.

Katanning Research Station Showcase: Projects And Initiatives, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock research reports

An overview of current projects run from Katanning Research Station.

Katanning Research Station is a sheep, pasture and cropping research hub. The digital agriculture technologies and management systems are designed to lift productivity and lower greenhouse gas emissions. The research includes the:

• genetics (breeding values) of feed intake efficiency in sheep
• impact of feed supplements and forage combinations on methane production of sheep
• evaluation of novel pasture species to develop livestock systems of green feed year round
• saltland rehabilitation program that aims to halt and reverse salinity (surface water management and groundwater drainage), and provide out-of-season feed (the planting of …

Evaluation Of Different Stocking Densities On Fattening Performance, Slaughter, Carcass, Meat Quality Characteristics, And Fatty Acid Composition In New Zealand Rabbits, Büşra Yaranoğlu, Mustafa Hi̇lmi̇ Yaranoğlu, Sali̇ha Uysal, Adnan Adi̇l Hi̇şmi̇oğullari

Turkish Journal of Veterinary & Animal Sciences

The aim of the research was to investigate the effects of different stocking densities on fattening performance, slaughter, carcass, meat quality characteristics, and fatty acid composition in New Zealand rabbits raised in cage conditions and also determine the ideal housing frequency so that yields were not adversely affected. In the study, 36 New Zealand rabbits were used. After weaning, the fattening was started and rabbits were put into cages at different stocking densities as 1 rabbit/cage, 2 rabbits/cage, and 3 rabbits/ cage. The live weight of rabbits and feed consumption data were taken every week. At the end of 8 …

Expression Of Hif-1Α, Caspase-3, P53, Hsp70, Hsp90, And Nrf2 In The Canine Spleen With Siderofibrotic Nodules: An Immunohistochemical And Immunofluorescence Perspective On Apoptosis, Ferroptosis, And Oxidative Stress, Ali Babanezhad-Gajouti, Amir Amniattalab

Turkish Journal of Veterinary & Animal Sciences

Splenic siderofibrotic nodules (SFNs) are benign tiny yellow-brownish foci found in dogs. Their pathogenesis is unclear whereas trauma and hemorrhage are assumed to be the possible causative factors. The spleen of 73 Iranian dogs of the mixed breed was examined for possible splenomegaly with SFNs. Ultrasonography, blood biochemistry, pathology, and immunohistochemistry revealed splenomegaly (n = 7), splenomegaly with SFNs (n = 6) and normal spleens (n = 60). There was no significant difference between the prevalence of splenomegaly with SFN among the examined dogs and their age or sex (p > 0.05). Ultrasonography represented a significant increase (p ≤ 0.01) in …

Determination Of Virulence Genes And Antibiotic Resistance Profiles Of Streptococcus Agalactiae Isolated From Buffalo Milk, Nevi̇n Tuzcu, Hasan Hüseyi̇n Hadi̇mli̇, Beatri̇z Padron

Turkish Journal of Veterinary & Animal Sciences

Mastitis is a contagious disease that causes significant milk loss and serious economic damage in dairy animals. Streptococcus agalactiae causes clinical and subclinical mastitis in animals and infections in humans, especially adults, and newborns. This study aimed to confirm the identification of S. agalactiae isolates by PCR in buffalo milk; to determine the antibiotic resistance and virulence genes by multiplex PCR. The material of this study consisted of 30 (12%) S. agalactiae strains identified by phenotypic, biochemical, and PCR methods (rRNA-16S) from 250 isolates with bacterial growth from milk samples of buffaloes with mastitis. In addition, the Kirby-Bauer disc diffusion …

Effect Of Feeding Frequency On Growth, Fecundity, And Meat Quality Of Abant Trout (Salmo Abanticus Tortonese, 1954) Broodstock, Mehmet Zeki̇ Alkan, Nadi̇r Başçinar

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to investigate the growth data, egg production and changes in the biochemical structure of meat of Abant trout depending on feeding frequency (once a day, twice a day, three times a day, four times a day) in order to contribute to the economy through the private sector. All fish in the groups were planned to be of similar weight at the beginning of the study. Individual fish weights were measured at the beginning of the study, and the average was found to be 224.87 ± 0.69 g. At the end of the study, the weights …

An Evaluation Of Canine Inguinal Hernias Containing The Uterus: Clinical Experience Of Four Cases (2017-2022) And Literature Review, Fi̇rdevs Bi̇nli̇, Fati̇h Büyükbudak, İpek İnan, Volkan Ferahoğlu, Anil Gürkan Aksu, Murat Findik, Serhan Serhat Ay

Turkish Journal of Veterinary & Animal Sciences

The aim of this study is to identify and summarize the evidence and risk factors of canine inguinal hernia containing the uterus (Inguinal Hysterocele; IH) etiology by presenting our clinical experience with a literature review. A total of 18 canine IH cases were evaluated in the study. Of these, four have not yet been published anywhere, two have been published before, and 12 were obtained through a literature review. According to the findings, 81.3% of the cases were small/medium races, 83.3% were ≥5 years old, and 72.7% were multiparous. Totally 83.3% of the cases were chronic and 72.2% of the …

Impact Of Black Soldier Fly (Hermetia Illucens) Fresh Meal On The Growth Performance, Digestive Enzymes, Hematology, And Intestinal Histology Of Cork Fish (Channa Striata), Boby Muslimin, Danang Yonarta, Eny Heriyati, Helmizuryani Helmizuryani, Meika Puspita Sari, Dewi Apriyanti, Rahmat Kurniawan

Turkish Journal of Veterinary & Animal Sciences

Black Soldier Fly (BSF) is an alternative ingredient for Fish Meal (FM) which is expected to reduce aquaculture operational costs and positively impact fish growth and health. The effect of BSF fresh meal on growth response, digestibility, intestinal hematology, and intestinal histology of cork fish was investigated. The BSF experiment with mixed percentages as treatments, by 20%, 50%, 80%, and 100%, was repeated four times. Furthermore, there were 50 individuals/m3 cork fish with an average weight of 1.6 ± 0.05 g distributed in each treatment. An increase in BSF influences a significant decrease in the Relative Growth Weight (RGW) and …

Comparison Of Bacterial Profile And Antibiotic Susceptibility Isolated From Surgical Site After Ventral Midline And Lateral Flank Approaches Of Ovariohysterectomy In Queens And Bitches, Ebru Karakaya Bi̇len, Serpi̇l Kahya Demi̇rbi̇lek, Öznur Yilmaz, Muhammet Ali̇ Karadağ, Havva Aner

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to identify the bacterial profile and antimicrobial susceptibility from surgical site cultures in queens and bitches undergoing ovariohysterectomy via ventral midline and lateral flank approaches. Healthy 22 queens and 21 bitches were assigned randomly either ventral midline or lateral flank approach for routine ovariohysterectomy. Surgical site samples were collected before the surgery and on the 1$^{st}$, 2$^{nd}$ , and 3 $^{rd}$days after the surgery for microbiological analysis. A total of 70 different strains were isolated from 50 (29%) of 172 samples. In both queens and bitches the total number of bacteria isolated from the …

Stereological Investigation Of Medulla Oblongata Volume In Norduz Sheep, Ozan Ertaş, Gamze Çakmak

Turkish Journal of Veterinary & Animal Sciences

In this study, it was aimed to stereologically examine the medulla oblongata volume of Norduz sheep in the Gürpınar District of Van Province. Upon receiving news from the sections in the Gürpınar region, we went to the region. A total of 12 Norduz sheep skulls, 6 females and 6 males, were received from the Norduz sheep slaughter in the region. After the brains of Norduz sheep were dissected, they were fixed as a whole in formaldehyde for 1 week. Medulla oblongatas were separated from the brain and kept in formaldehyde for 1 week. Tissues obtained by sequential random sampling from …

In Vitro Antimicrobial And Antibiofilm-Forming Activities Of Origanum Munzurense Plant Extract On Some Foodborne Gram-Negative Bacteria, Hali̇l Yalçin, Esra Zeybek, Oktay Özkan, Asim Kart

Turkish Journal of Veterinary & Animal Sciences

lants are an important source for the discovery of antimicrobial formulations due to the many biologically active components they contain. In this study, in vitro antimicrobial and antibiofilm forming activities of ethanolic extract of Origanum munzurense were investigated against some strains of foodborne pathogenic bacteria. To investigate the antimicrobial activity, the minimal inhibitory concentration values against the selected bacteria and antibiofilm-forming activity were tested with the liquid microdilution method and microplate method, respectively. MICs of Origanum munzurense extract against the bacteria tested are 32 mg/mL for Escherichia coli O157: H7 ATCC 35150, 32 mg/mL for Escherichia coli O157: H7 ATCC …

The First Data Of A Newly Recorded Native Goat Genotype Called Manavlı In Türkiye: Growth Traits Of Kids, Aykut Asim Akbaş, Mustafa Saatci, Özkan Elmaz, Can Meti̇n Yazici

Turkish Journal of Veterinary & Animal Sciences

This study aimed to define growth traits of kids named Manavlı goat from six flocks under the breeder?s conditions in Denizli Province. The average birth weights of the kids were 3.63 kg, 4.28 kg, 3.85 kg, 4.11 kg, 3.83 kg, and 3.64 kg for the six flocks, respectively. The average live weights of male and female kids on 60th, 90th, and 150th day of age were 16.50 kg, 22.35 kg, and 34.82 kg and 15.33 kg, 20.14 kg, and 32.06 kg, respectively (p < 0.05). While the average live weights of single kids on 60th, 90th were 16.70 kg and 21.96 kg, the values for twins were 15.13 kg and 21.03 kg, respectively (p < 0.05). Body measurements of male kids such as height at withers, rump height, rump width, body length, heart girth, head length, ear length, tail length, and front and back wrist girth were measured as 70.26 cm, 70.21 cm, 16.23 cm, 66.80 cm, 70.11 cm, 19.51 cm, 22.16 cm, 18.59 cm, 11.85 cm, and 11.57 cm, respectively, on the 150th day of age. This study is the first research related to growth traits of Manavlı kids, for which no features have been reported to date. It was thought that the results obtained will help to characterise the Manavlı goat and will provide a database for the following studies.

Euscorpius Sulfur Sp. N. (Scorpiones: Euscorpiidae), A New Cave Scorpion From Albania And Northwestern Greece, František Kovařík, Marek Audy, Serban M. Sarbu, Victor Fet

Euscorpius

A new species of cave scorpion, Euscorpius sulfur sp. n. (Scorpiones: Euscorpiidae) from Albania and northwestern Greece is described, fully complemented with color photographs of both live and preserved specimens, as well as their habitats.

The Devil You Know And The Devil You Don’T: Current Status And Challenges Of Bovine Tuberculosis Eradication In The United States, Daniel J. O'Brien, Tyler C. Thacker, Liliana C.M. Salvador, Anthony G. Duffiney, Suelee Robbe‑Austerman, Mark S. Camacho, Jason E. Lombard, Mitchell V. Palmer

United States Department of Agriculture Wildlife Services: Staff Publications

Having entered into its second century, the eradication program for bovine tuberculosis (bTB, caused by Mycobacterium bovis) in the United States of America occupies a position both enviable and daunting. Excepting four counties in Michigan comprising only 6109 km2 (0.06% of US land area) classified as Modified Accredited, as of April 2022 the entire country was considered Accredited Free of bTB by the US Department of Agriculture for cattle and bison. On the surface, the now well-described circumstances of endemic bTB in Michigan, where white-tailed deer (Odocoileus virginianus) serve as a free-ranging wildlife maintenance host, may appear to be …

Resource Assessment Report For Western Australian Salmon In Western Australia, Rodney Duffy Dr., Bianca Brooks, Alex Hesp, Amber Quinn, Blaine Hodgson, Marcus Newman

Fisheries Research Reports

This document provides a cumulative description and assessment of the Western Australian Salmon Resource (Resource) and all fishing activities (i.e., fisheries / fishing sectors) affecting this Resource in WA. The overall Resource comprises a single species, Western Australian salmon, caught within WA, in both the WCB and SCB. This species is primarily captured during the annual autumn ‘salmon run’ by recreational fishers and commercial beach seine fishers that operate on the South Coast. The report contains information relevant to assist the assessment of the Resource against Environment Protection and Biodiversity Conservation (EPBC) Act export approval requirements / the Marine Stewardship …

Dna Methylation Networks Underlying Mammalian Traits, Amin Haghani

Department of Animal Science: Faculty Publications

INTRODUCTION: Comparative epigenomics is an emerging field that combines epigenetic signatures with phylogenetic relationships to elucidate species characteristics such as maximum life span. For this study, we generated cytosine DNA methylation (DNAm) profiles (n = 15,456) from 348mammalian species using amethylation array platform that targets highly conserved cytosines.

RATIONALE: Nature has evolved mammalian species of greatly differing life spans. To resolve the relationship ofDNAmwith maximum life span and phylogeny, we performed a largescale cross-species unsupervised analysis. Comparative studies in many species enables the identification of epigenetic correlates of maximum life span and other traits.

RESULTS: We …

Universal Dna Methylation Age Across Mammalian Tissues, A. T. Lu, Jessica Lynn Petersen, Steve Horvath, Et Al.

Department of Animal Science: Faculty Publications

Aging, often considered a result of random cellular damage, can be accurately estimated using DNA methylation profiles, the foundation of pan-tissue epigenetic clocks. Here, we demonstrate the development of universal pan-mammalian clocks, using 11,754 methylation arrays from our Mammalian Methylation Consortium, which encompass 59 tissue types across 185 mammalian species. These predictive models estimate mammalian tissue age with high accuracy (r > 0.96). Age deviations correlate with human mortality risk, mouse somatotropic axis mutations and caloric restriction. We identified specific cytosines with methylation levels that change with age across numerous species. These sites, highly enriched in polycomb repressive complex 2-binding locations, …