Physical and Environmental Geography Commons^™

Using Redundant Primer Sets To Detect Multiple Native Alaskan Fish Species From Environmental Dna, Damian Menning, Trey Simmons, Sandra Talbot

United States National Park Service: Publications

Accurate and timely data regarding freshwater fish communities is important for informed decision-making by local, state, tribal, and federal land and resource managers; however, conducting traditional gear-based fish surveys can be an expensive and time-consuming process, particularly in remote areas, like those that characterize much of Alaska. To help address this challenge, we developed and tested five multi-species environmental DNA (eDNA) primer sets for the simultaneous detection of up to 37 target fish species in a single sample. Using these primer sets can reduce the cost and time needed to perform future studies of fish communities. Our results comparing multiple …