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Full-Text Articles in Physical and Environmental Geography
Using Redundant Primer Sets To Detect Multiple Native Alaskan Fish Species From Environmental Dna, Damian Menning, Trey Simmons, Sandra Talbot
Using Redundant Primer Sets To Detect Multiple Native Alaskan Fish Species From Environmental Dna, Damian Menning, Trey Simmons, Sandra Talbot
United States National Park Service: Publications
Accurate and timely data regarding freshwater fish communities is important for informed decision-making by local, state, tribal, and federal land and resource managers; however, conducting traditional gear-based fish surveys can be an expensive and time-consuming process, particularly in remote areas, like those that characterize much of Alaska. To help address this challenge, we developed and tested five multi-species environmental DNA (eDNA) primer sets for the simultaneous detection of up to 37 target fish species in a single sample. Using these primer sets can reduce the cost and time needed to perform future studies of fish communities. Our results comparing multiple …