Physics Commons^™

Nadph-Cytochrome P450 Oxidoreductase: Extraction Of The Full-Length Protein And Methyl-Trosy Nmr Of The Soluble Mutants, Sara Ali Jamal Arafeh

Master's Theses (2009 -)

NADPH-cytochrome p450 oxidoreductase (CYPOR) is a membrane-bound protein in living cells. CYPOR delivers electrons to cytochrome p450 proteins (CYPs) to catalyze metabolism of drugs and synthesis of steroids. Extraction and solubilization of CYPOR from the membrane is typically done with the TritonX-100 detergent. The amount of the solubilized protein by this detergent, however, remains relatively low to structurally analyze CYPOR with NMR spectroscopy. The goal of the first project in this thesis was to optimize the amount of the extracted CYPOR from the E. coli membrane using various detergents and additives. To this aim, non-ionic detergents with variable hydrophobicity (TritonX-100, …

Functional And Structural Studies Of Cytochromes P450 By Resonance Raman Spectroscopy, Yilin Liu

Dissertations (1934 -)

Cytochrome P450 is a broad class of heme monooxygenase enzymes which catalyze various oxidative transformations. There are two main kinds of mammalian P450s: steroidogenic and drug metabolizing P450s. The first project involves a steroidogenic P450, CYP17A1, occupying a central role in the biosynthesis of steroid hormones. It catalyzes hydroxylation reaction on pregnenolone and progesterone, generating 17OH-pregnenolone and 17OH-progesterone, presumably utilizing a “Compound I” species. However, these hydroxylated products can be further processed in a second oxidative cycle to cleave the C17–C20 bond to form dehydroepiandrosterone or androstenedione, respectively, a crucial step in androgen production. Interestingly, it is well known that …

Characterization Of The Shock Wave Structure In Water, Emilie Teitz

Master's Theses (2009 -)

The scientific community is interested in furthering the understanding of shock wave structures in water, given its implications in a wide range of applications; from researching how shock waves penetrate unwanted body tissues to studying how humans respond to blast waves. Shock wave research on water has existed for over five decades. Previous studies have investigated the shock response of water at pressures ranging from 1 to 70 GPa using flyer plate experiments. This report differs from previously published experiments in that the water was loaded to shock pressures ranging from 0.36 to 0.70 GPa. The experiment also utilized tap …

A Study Of Mesoscale Simulations For Planar Shock Experiments On Heterogeneous Granular Materials, Merit Grayson Schumaker

Master's Theses (2009 -)

There is an interest in producing accurate and reliable computer simulations to predict the dynamic behavior of heterogeneous materials and to use these simulations to gain further insight into experimental results. In so doing, a more complete understanding of the multiple-length scales involved in heterogeneous material compaction can be obtained. Mesoscale computer simulations of dynamically shocked materials have proven to be a beneficial resource in unraveling data not observed in planar shock impact experiments, such as stress and temperature interactions between grains. The modeled mono-dispersed geometry of particles, the densities of each material, equations of state, material properties and many …

Coupling Into Waveguide Evanescent Modes With Applications In Electron Paramagnetic Resonance, Jason Walter Sidabras

Master's Theses (2009 -)

The use of analytical and numerical techniques in solving the coupling of evanescent modes in a microwave waveguide through slots can be optimized to create a uniform magnetic field excitation on axis within a waveguide. This work has direct applications in Electron Paramagnetic Resonance (EPR) where a 100~kHz time-varying magnetic field is incident on a sample contained in a microwave cavity. Typical cavity designs do not take into consideration the uniformity of the 100~kHz field modulation and assume it to be uniform enough over the sample region from quasi-static principles. This work shows otherwise and uses Ansoft (Pittsburgh, PA) High …

Educative Value And Construction Of Laboratory Equipment For Physics, With Emphasis On An Optical Screen, Warren D. Conrad

Bachelors’ Theses

In the preparation of this thesis the writer has constantly kept in mind three distinct purposes: a) to show the place and importance of the lecture-demonstration in the teaching of physics; b) to provide the teacher of physics in the small high school a means whereby he may equip a laboratory at the smallest possible cost; and c) to describe in detail the construction of an optical screen for purposes of demonstration, indicating what can be done. in the building of home-made physics apparatus.

The Application Of Differential Equations To The Laws Of Physics, Sherman Baker

Bachelors’ Theses

In this treatment of the mathematical expression of physical laws, all proofs and expositions of problems that are taken from particular books are acknowledged in the text. Formulas and proofs that are given in a conventional form by authors generally and are the property of no single man are not acknowledged. Such are the alternating current equations, the Mass Action formulas, parts of the thermionic current discussion, and Fourier's equation for the flow of heat.

Some preparation in the sciences on the part of the reader is taken for granted in the presentation of the matter on this thesis. The …

Faraday's Laws Of Electrolysis, M. Aquinas

Bachelors’ Theses

Physics has no equal, perhaps, in bringing the student to an appreciation of the beauty, the order and the harmony or the universe, and thus drawing the heart and the mind to God whose unbounded powers executed the eternal and marvelous wonders that are all about us.

It is intensely fascinating to take peeps into the "laboratories of God" and wonder what will be the next secret that He will let man in on. It seems as the ages roll by He gradually allows us to share more and more in His secrets or nature; He appears to be slowly …