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Articles 31 - 60 of 91
Full-Text Articles in Physics
Microfabricated Nanotopological Surfaces For Study Of Adhesion-Dependent Cell Mechanosensitivity, Weiqiang Chen, Yubing Sun, Jianping Fu
Microfabricated Nanotopological Surfaces For Study Of Adhesion-Dependent Cell Mechanosensitivity, Weiqiang Chen, Yubing Sun, Jianping Fu
Weiqiang Chen
Cells exhibit high sensitivity and diverse responses to the intrinsic nanotopography of the extracellular matrix through their nanoscale cellular sensing machinery. A simple microfabrication method for precise control and spatial patterning of the local nanoroughness on glass surfaces by using photolithography and reactive ion etching is reported. It is demonstrated that local nanoroughness as a biophysical cue could regulate a diverse array of NIH/3T3 fi broblast behaviors, including cell morphology, adhesion, proliferation, migration, and cytoskeleton contractility. The capability to control and further predict cellular responses to nanoroughness might suggest novel methods for developing biomaterials mimicking nanotopographic structures in vivo for …
Surface-Micromachined Microfiltration Membranes For Efficient Isolation And Functional Immunophenotyping Of Subpopulations Of Immune Cells, Weiqiang Chen, Nien-Tsu Huang, Boram Oh, Raymond H. W. Lam, Rong Fan, Timothy T. Cornell, Thomas P. Shanley, Katsuo Kurabayashi, Jianping Fu
Surface-Micromachined Microfiltration Membranes For Efficient Isolation And Functional Immunophenotyping Of Subpopulations Of Immune Cells, Weiqiang Chen, Nien-Tsu Huang, Boram Oh, Raymond H. W. Lam, Rong Fan, Timothy T. Cornell, Thomas P. Shanley, Katsuo Kurabayashi, Jianping Fu
Weiqiang Chen
An accurate measurement of the immune status in patients with immune system disorders is critical in evaluating the stage of diseases and tailoring drug treatments. The functional cellular immunity test is a promising method to establish the diagnosis of immune dysfunctions. The conventional functional cellular immunity test involves measurements of the capacity of peripheral blood mononuclear cells to produce pro-inflammatory cytokines when stimulated ex vivo. However, this “bulk” assay measures the overall reactivity of a population of lymphocytes and monocytes, making it difficult to pinpoint the phenotype or real identity of the reactive immune cells involved. In this research, we …
Mechaniczny Rozdział Faz Proj., Wojciech M. Budzianowski
Mechaniczny Rozdział Faz Proj., Wojciech M. Budzianowski
Wojciech Budzianowski
No abstract provided.
Challenges And Prospects Of Processes Utilising Carbonic Anhydrase For Co2 Separation, Patrycja Szeligiewicz, Wojciech M. Budzianowski
Challenges And Prospects Of Processes Utilising Carbonic Anhydrase For Co2 Separation, Patrycja Szeligiewicz, Wojciech M. Budzianowski
Wojciech Budzianowski
This article provides an analysis of processes for separation CO2 by using carbonic anhydrase enzyme with particular emphasis on reactive-membrane solutions. Three available processes are characterised. Main challenges and prospects are given. It is found that in view of numerous challenges practical applications of these processes will be difficult in near future. Further research is therefore needed for improving existing processes through finding methods for eliminating their main drawbacks such as short lifetime of carbonic anhydrase or low resistance of reactive membrane systems to impurities contained in flue gases from power plants.
Determination Of Kinetic Parameters From The Thermogravimetric Data Set Of Biomass Samples, Karol Postawa, Wojciech M. Budzianowski
Determination Of Kinetic Parameters From The Thermogravimetric Data Set Of Biomass Samples, Karol Postawa, Wojciech M. Budzianowski
Wojciech Budzianowski
This article describes methods of the determination of kinetic parameters from the thermogravimetric data set of biomass samples. It presents the methodology of the research, description of the needed equipment, and the method of analysis of thermogravimetric data. It describes both methodology of obtaining quantitative data such as kinetic parameters as well as of obtaining qualitative data like the composition of biomass. The study is focused mainly on plant biomass because it is easy in harvesting and preparation. Methodology is shown on the sample containing corn stover which is subsequently pyrolysed. The investigated sample show the kinetic of first order …
Correct Spectral Conversion Between Surface‐Enhanced Raman And Plasmon Resonance Scattering From Nanoparticle Dimers For Single‐Molecule Detection, Kyuwan Lee
Kyuwan Lee
Simultaneous measurement of surface-enhanced Raman scattering (SERS) and localized surface plasmon resonance (LSPR) in nanoparticle dimers presents outstanding opportunities in molecular identification and in the elucidation of physical properties, such as the size, distance, and deformation of target species. SERS–LSPR instrumentation exists and has been used under limited conditions, but the extraction of SERS and LSPR readouts from a single measurement is still a challenge. Herein, the extraction of LSPR spectra from SERS signals is reported and a tool for measuring the interparticle distance from Raman enhancement data by the standardization of the SERS signal is proposed. The SERS nanoruler …
Toward High-Performance Nanostructured Thermoelectric Materials: The Progress Of Bottom-Up Solution Chemistry Approaches, Yixin Zhao, Jeffrey S. Dyck, Clemens Burda
Toward High-Performance Nanostructured Thermoelectric Materials: The Progress Of Bottom-Up Solution Chemistry Approaches, Yixin Zhao, Jeffrey S. Dyck, Clemens Burda
Jeffrey Dyck
Significant research effort has recently gone into the synthesis of thermoelectric nanomaterials through different chemical approaches since nanomaterials chemistry became a promising strategy for improving thermoelectric performance. Different thermoelectric nanocrystals, especially PbTe, Bi2Te3 and CoSb3, with various compositions and morphologies have been successfully prepared by solvo/hydrothermal, electrochemical, and ligand-based synthesis methods. Such nanoscale materials show not only substantial reduction in thermal conductivity due to increased phonon scattering at nanoscale grain boundaries and lower densities of phonon states but possibly also an enhancement in thermopower due to electronic quantum size effects. More recently, the notoriously low power factors of thermoelectric nanomaterials …
Structure Inhomogeneities, Shallow Defects, And Charge Transport In The Series Of Thermoelectric Materials K2bi8−Xsbxse13, Jeffrey S. Dyck, T. Kyratski, E. Hatzikraniotis, K. M. Paraskevopoulos, C. D. Malliakas, C. Uher, M. G. Kanatzidis
Structure Inhomogeneities, Shallow Defects, And Charge Transport In The Series Of Thermoelectric Materials K2bi8−Xsbxse13, Jeffrey S. Dyck, T. Kyratski, E. Hatzikraniotis, K. M. Paraskevopoulos, C. D. Malliakas, C. Uher, M. G. Kanatzidis
Jeffrey Dyck
The charge transport properties of the low-dimensional thermoelectric materials K2Bi8-xSbxSe13 (02Bi8-xSbxSe13 was analyzed on the basis of the classical semiconductor theory and discussed in the context of recent band calculations. The results suggest that the K2Bi8-xSbxSe13 materials possess coexisting domains with semimetallic and semiconducting characters whose ratio is influenced by the value of x and by local defects. The extent and relative distribution of these domains control the charge transport properties. Electron diffraction experiments performed on samples of K2Bi8-xSbxSe13 with x=1.6 show evidence for such domains by indicating regions with long range ordering of K+/Bi3+ atoms and regions with increased …
Nanoroughened Surfaces For Efficient Capture Of Circulating Tumor Cells Without Using Capture Antibodies, Weiqiang Chen, Shinuo Weng, Feng Zhang, Steven Allen, Xiang Li, Liwei Bao, Raymond H. W. Lam, Jill A. Macoska, Sofia D. Merajver, Jianping Fu
Nanoroughened Surfaces For Efficient Capture Of Circulating Tumor Cells Without Using Capture Antibodies, Weiqiang Chen, Shinuo Weng, Feng Zhang, Steven Allen, Xiang Li, Liwei Bao, Raymond H. W. Lam, Jill A. Macoska, Sofia D. Merajver, Jianping Fu
Weiqiang Chen
Circulating tumor cells (CTCs) detached from both primary and metastatic lesions represent a potential alternative to invasive biopsies as a source of tumor tissue for the detection, characterization and monitoring of cancers. Here we report a simple yet effective strategy for capturing CTCs without using capture antibodies. Our method uniquely utilized the differential adhesion preference of cancer cells to nanorough surfaces when compared to normal blood cells and thus did not depend on their physical size or surface protein expression, a significant advantage as compared to other existing CTC capture techniques.
Double Layer In Ionic Liquids: Overscreening Versus Crowding, Martin Z. Bazant, Brian D. Storey, Alexei A. Kornyshev
Double Layer In Ionic Liquids: Overscreening Versus Crowding, Martin Z. Bazant, Brian D. Storey, Alexei A. Kornyshev
Brian Storey
We develop a simple Landau-Ginzburg-type continuum theory of solvent-free ionic liquids and use it to predict the structure of the electrical double layer. The model captures overscreening from short-range correlations, dominant at small voltages, and steric constraints of finite ion sizes, which prevail at large voltages. Increasing the voltage gradually suppresses overscreening in favor of the crowding of counterions in a condensed inner layer near the electrode. This prediction, the ion profiles, and the capacitance-voltage dependence are consistent with recent computer simulations and experiments on room-temperature ionic liquids, using a correlation length of order the ion size.
Tension-Induced Straightening Transition Of Self-Assembled Helical Ribbons, Yevgeniya V. Zastavker, Brice Smith, George B. Benedek
Tension-Induced Straightening Transition Of Self-Assembled Helical Ribbons, Yevgeniya V. Zastavker, Brice Smith, George B. Benedek
Yevgeniya V. Zastavker
Helical ribbons with pitch angles of either 11° or 54° self-assemble in a wide variety of quaternary surfactant-phospholipid/fatty acid-sterol-water systems. By elastically deforming these helices, we examined their response to uniaxial forces. Under sufficient tension, a low pitch helix reversibly separates into a straight domain with a pitch angle of 90° and a helical domain with a pitch angle of 16.5°. Using a newly developed continuum elastic free energy model, we have shown that this phenomenon can be understood as a first order mechanical phase transition.
Nanotopography Influences Adhesion, Spreading, And Self-Renewal Of Human Embryonic Stem Cells, Weiqiang Chen, Luis G. Villa-Diaz, Yubing Sun, Shinuo Weng, Jin Koo Kim, Raymond H. W. Lam, Lin Han, Rong Fan, Paul H. Krebsbach, Jianping Fu
Nanotopography Influences Adhesion, Spreading, And Self-Renewal Of Human Embryonic Stem Cells, Weiqiang Chen, Luis G. Villa-Diaz, Yubing Sun, Shinuo Weng, Jin Koo Kim, Raymond H. W. Lam, Lin Han, Rong Fan, Paul H. Krebsbach, Jianping Fu
Weiqiang Chen
Human embryonic stem cells (hESCs) have great potentials for future cell-based therapeutics. However, their mechanosensitivity to biophysical signals from the cellular microenvironment is not well characterized. Here we introduced an effective microfabrication strategy for accurate control and patterning of nanoroughness on glass surfaces. Our results demonstrated that nanotopography could provide a potent regulatory signal over different hESC behaviors, including cell morphology, adhesion, proliferation, clonal expansion, and self-renewal. Our results indicated that topological sensing of hESCs might include feedback regulation involving mechanosensory integrin-mediated cell matrix adhesion, myosin II, and E-cadherin. Our results also demonstrated that cellular responses to nanotopography were cell-type …
Reconciling Molecular Regulatory Mechanisms With Noise Patterns Of Bacterial Metabolic Promoters In Induced And Repressed States, Matthew L. Ferguson, Dominique Le Coq, Matthieu Jules, Stéphane Aymerich, Ovidiu Radulescu, Nathalie Declerck, Catherine A. Royer
Reconciling Molecular Regulatory Mechanisms With Noise Patterns Of Bacterial Metabolic Promoters In Induced And Repressed States, Matthew L. Ferguson, Dominique Le Coq, Matthieu Jules, Stéphane Aymerich, Ovidiu Radulescu, Nathalie Declerck, Catherine A. Royer
Matthew L. Ferguson
Assessing gene expression noise in order to obtain mechanistic insights requires accurate quantification of gene expression on many individual cells over a large dynamic range. We used a unique method based on 2-photon fluorescence fluctuation microscopy to measure directly, at the single cell level and with single-molecule sensitivity, the absolute concentration of fluorescent proteins produced from the two Bacillus subtilis promoters that control the switch between glycolysis and gluconeogenesis. We quantified cell-to-cell variations in GFP concentrations in reporter strains grown on glucose or malate, including very weakly transcribed genes under strong catabolite repression. Results revealed strong transcriptional bursting, particularly for …
Ogólnotechniczne Podstawy Biotechnologii Z Elementami Grafiki Inżynierskiej Ćw., Wojciech M. Budzianowski
Ogólnotechniczne Podstawy Biotechnologii Z Elementami Grafiki Inżynierskiej Ćw., Wojciech M. Budzianowski
Wojciech Budzianowski
No abstract provided.
Materiały Odstresowujące, Wojciech M. Budzianowski
Materiały Odstresowujące, Wojciech M. Budzianowski
Wojciech Budzianowski
No abstract provided.
Hydrogen Production From Biogas By Oxy-Reforming: Reaction System Analysis, Aleksandra Terlecka, Wojciech M. Budzianowski
Hydrogen Production From Biogas By Oxy-Reforming: Reaction System Analysis, Aleksandra Terlecka, Wojciech M. Budzianowski
Wojciech Budzianowski
Oxy-reforming is emerging as an interesting alternative to conventional methods of hydrogen generation. The current article characterises this process through analysis of individual reactions: SMR (steam methane reforming), WGS (water gas shift) and CPO (catalytic partial oxidation). Analyses relate to optimisation of thermal conditions thus enabling cost-effectivenes of the process.
Absolute Quantification Of Gene Expression In Individual Bacterial Cells Using Two-Photon Fluctuation Microscopy, Matthew L. Ferguson, Dominique Le Coq, Matthieu Jules, Stéphane Aymerich, Nathalie Declerck, Catherine A. Royer
Absolute Quantification Of Gene Expression In Individual Bacterial Cells Using Two-Photon Fluctuation Microscopy, Matthew L. Ferguson, Dominique Le Coq, Matthieu Jules, Stéphane Aymerich, Nathalie Declerck, Catherine A. Royer
Matthew L. Ferguson
Quantification of promoter activity or protein expression in gene regulatory networks is generally achieved via measurement of fluorescent protein (FP) intensity, which is related to the true FP concentration by an unknown scaling factor, thereby limiting analysis and interpretation. Here, using approaches originally developed for eukaryotic cells, we show that two-photon (2p) fluorescence fluctuation microscopy, specifically scanning number and brightness (sN&B) analysis, can be applied to determine the absolute concentrations of diffusing FPs in live bacterial cells. First, we demonstrate the validity of the approach, despite the small size of the bacteria, using the central pixels and spatial averaging. We …
Photolithographic Surface Micromachining Of Polydimethylsiloxane (Pdms), Weiqiang Chen, Raymond H. W. Lam, Jianping Fu
Photolithographic Surface Micromachining Of Polydimethylsiloxane (Pdms), Weiqiang Chen, Raymond H. W. Lam, Jianping Fu
Weiqiang Chen
A major technical hurdle in microfluidics is the difficulty in achieving high fidelity lithographic patterning on polydimethylsiloxane (PDMS). Here, we report a simple yet highly precise and repeatable PDMS surface micromachining method using direct photolithography followed by reactive ion etching (RIE). Our method to achieve surface patterning of PDMS applied an O2 plasma treatment to PDMS to activate its surface to overcome the challenge of poor photoresist adhesion on PDMS for photolithography. Our photolithographic PDMS surface micromachining technique is compatible with conventional soft lithography techniques and other silicon-based surface and bulk micromachining methods. To illustrate the general application of our …
Violence On The Brain: A Critique Of Neuroscience In Criminal Law, Amanda C. Pustilnik
Violence On The Brain: A Critique Of Neuroscience In Criminal Law, Amanda C. Pustilnik
Amanda C Pustilnik
Is there such a thing as a criminally "violent brain"? Does it make sense to speak of "the neurobiology of violence" or the "psychopathology of crime"? Is it possible to answer on a physiological level what makes one person engage in criminal violence and another not, under similar circumstances? This Article first demonstrates parallels between certain current claims about the neurobiology of criminal violence and past movements that were concerned with the law and neuroscience of violence: phrenology, Lombrosian biological criminology, and lobotomy. It then engages in a substantive review and critique of several current claims about the neurological bases …
Dna− Gold Nanoparticle Reversible Networks Grown On Cell Surface Marker Sites: Application In Diagnostics, Kyuwan Lee
Dna− Gold Nanoparticle Reversible Networks Grown On Cell Surface Marker Sites: Application In Diagnostics, Kyuwan Lee
Kyuwan Lee
Effective identification of breast cancer stem cells (CSC) benefits from a multiplexed approach to detect cell surface markers that can distinguish this subpopulation, which can invade and proliferate at sites of metastasis. We present a new approach for dual-mode sensing based on targeting using pointer and signal enhancement using enhancer particle networks for detection by surface plasmon resonance (SPR) and surface-enhanced Raman scattering (SERS). We demonstrate our concept to detect cell surface markers, CD44 and CD24, in three breast cancer cell lines to identify a CD44+/CD24− subpopulation of CSCs. The designed network structure can be well-controlled and has improved sensitivity …
Termodynamika Procesowa (Dla Me Aparatura Procesowa) Ćw., Wojciech M. Budzianowski
Termodynamika Procesowa (Dla Me Aparatura Procesowa) Ćw., Wojciech M. Budzianowski
Wojciech Budzianowski
No abstract provided.
The Analysis Of Heat Transfer In A Gas-Gas Heat Exchanger Operated Under A Heat-Recirculating Mode, Mariusz Salaniec, Wojciech M. Budzianowski
The Analysis Of Heat Transfer In A Gas-Gas Heat Exchanger Operated Under A Heat-Recirculating Mode, Mariusz Salaniec, Wojciech M. Budzianowski
Wojciech Budzianowski
The present paper presents the analysis of heat transfer in a gas-gas heat exchanger operated in a heat-recirculating mode.
An Overview Of Technologies For Upgrading Of Biogas To Biomethane, Wojciech M. Budzianowski
An Overview Of Technologies For Upgrading Of Biogas To Biomethane, Wojciech M. Budzianowski
Wojciech Budzianowski
The present contribution presents an overview of technologies available for upgrading of biogas to biomethane. Technologies under study include pressure swing adsorption (PSA), high-pressure water wash (HPWW), reactive absorption (RA), physical absorption (PA), membrane separation (MS) and cryogenic separation (CS).
Influence Of Energy Policy On The Rate Of Implementation Of Biogas Power Plants In Germany During The 2001-2010 Decade, Izabela Chasiak, Wojciech M. Budzianowski
Influence Of Energy Policy On The Rate Of Implementation Of Biogas Power Plants In Germany During The 2001-2010 Decade, Izabela Chasiak, Wojciech M. Budzianowski
Wojciech Budzianowski
The current article describes energy policy tools, which caused intensive development of biogas-based power generation in Germany during the 2001-2010 decade. The German system of financial support to biogas power plants is presented in details. It is shown that in Germany, i.e. in a country characterised by similar climate and potentials to renewable energy to Poland, biogas power plants cover 10,7% of electricity demands in 2010, while all renewable energy sources cover only 5,4% of electricity demands. It is emphasised that under favourable Polish energy policy, the development of biogas energy can be very rapid.
Silver Nanosphere Sers Probes For Sensitive Identification Of Pathogens, Kyuwan Lee
Silver Nanosphere Sers Probes For Sensitive Identification Of Pathogens, Kyuwan Lee
Kyuwan Lee
The identification and timely detection of pathogenic bacteria is critical to ensuring safe food, health, and water. Although surface enhanced Raman scattering (SERS) methods have been used for pathogen characterization and single molecule sensing, the challenge of detecting pathogens in very low numbers using an optimal substrate that is sensitive and reproducible is still a challenge. In this report, we have developed and explored a novel SERS active substrate of 60−80 nm diameter through the assembly of Ag nanocrystals (AgNCs) into Ag nanospheres (AgNSs). A finite difference time domain (FDTD) analysis of the electromagnetic field produced by these structures and …
Energetyka Niskoemisyjna, Wojciech M. Budzianowski
Energetyka Niskoemisyjna, Wojciech M. Budzianowski
Wojciech Budzianowski
No abstract provided.
Physical Basis Of The Inducer-Dependent Cooperativity Of The Central Glycolytic Genes Repressor/Dna Complex, Denis Chaix, Matthew L. Ferguson, Cedric Atmanene, Alain Van Dorsselaer, Sarah Sanglier-Cianférani, Catherine A. Royer, Nathalie Declerck
Physical Basis Of The Inducer-Dependent Cooperativity Of The Central Glycolytic Genes Repressor/Dna Complex, Denis Chaix, Matthew L. Ferguson, Cedric Atmanene, Alain Van Dorsselaer, Sarah Sanglier-Cianférani, Catherine A. Royer, Nathalie Declerck
Matthew L. Ferguson
The Central glycolytic genes Repressor (CggR) from Bacillus subtilis belongs to the SorC family of transcription factors that control major carbohydrate metabolic pathways. Recent studies have shown that CggR binds as a tetramer to its tandem operator DNA sequences and that the inducer metabolite, fructose 1,6-bisphosphate (FBP), reduces the binding cooperativity of the CggR/DNA complex. Here, we have determined the effect of FBP on the size, shape and stoichiometry of CggR complexes with full-length and half-site operator sequence by small-angle X-ray scattering, size-exclusion chromatography, fluorescence cross-correlation spectroscopy and noncovalent mass spectrometry (MS). Our results show that CggR forms a compact …
Hydration Dynamics At Fluorinated Protein Surfaces, Oh-Hoon Kwon, Tae Hyeon Yoo, Christina M. Othon, James A. Van Deventer, David A. Tirrell, Ahmed H. Zewail
Hydration Dynamics At Fluorinated Protein Surfaces, Oh-Hoon Kwon, Tae Hyeon Yoo, Christina M. Othon, James A. Van Deventer, David A. Tirrell, Ahmed H. Zewail
Christina M Othon
Water-protein interactions dictate many processes crucial to protein function including folding, dynamics, interactions with other biomolecules, and enzymatic catalysis. Here we examine the effect of surface fluorination on water-protein interactions. Modification of designed coiled-coil proteins by incorporation of 5,5,5-trifluoroleucine or (4S)-2-amino-4-methylhexanoic acid enables systematic examination of the effects of side-chain volume and fluorination on solvation dynamics. Using ultrafast fluorescence spectroscopy, we find that fluorinated side chains exert electrostatic drag on neighboring water molecules, slowing water motion at the protein surface.
Theoretical Description Of A Dna-Linked Nanoparticle Self-Assembly, Chia Wei Hsu, Francesco Sciortino, Francis Starr
Theoretical Description Of A Dna-Linked Nanoparticle Self-Assembly, Chia Wei Hsu, Francesco Sciortino, Francis Starr
Francis Starr
No abstract provided.
Grafika Inżynierska Ćw., Wojciech M. Budzianowski
Grafika Inżynierska Ćw., Wojciech M. Budzianowski
Wojciech Budzianowski
No abstract provided.