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Full-Text Articles in Physics
Heterogeneous Rotational Diffusion Of A Fluorescent Probe In Lipid Monolayers, Christina M. Othon
Heterogeneous Rotational Diffusion Of A Fluorescent Probe In Lipid Monolayers, Christina M. Othon
Christina M Othon
The rotational correlation time of the lipid probe 1-palmitoyl-2-{6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphocholine (NBD-PC) is measured using fluorescence anisotropy for two lipid species. We measure the rotational diffusion in a monolayer of 1,2-Didecanoyl-sn-glycero-3-phosphocholine (DPPC) which displays a phase transition at room temperature from the liquid expanded to the liquid-condensed phase. The constant rotational diffusion of the probe throughout the phase transition reflects the measurement of dynamics in only the liquid-expanded phase. We contrast the dynamic changes during this phase coexistence to the continuous density increase observed in 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) at room temperature. We observe a non-exponential decay of the probe diffusion consistent with heterogeneity …
Termodynamika Procesowa I Techniczna Lab., Wojciech M. Budzianowski
Termodynamika Procesowa I Techniczna Lab., Wojciech M. Budzianowski
Wojciech Budzianowski
No abstract provided.
Tematyka Prac Dyplomowych Dla Studentów Wydziału Mechaniczno-Energetycznego Pwr., Wojciech M. Budzianowski
Tematyka Prac Dyplomowych Dla Studentów Wydziału Mechaniczno-Energetycznego Pwr., Wojciech M. Budzianowski
Wojciech Budzianowski
No abstract provided.
Tematyka Prac Dyplomowych Dla Studentów Wydziału Chemicznego Pwr., Wojciech M. Budzianowski
Tematyka Prac Dyplomowych Dla Studentów Wydziału Chemicznego Pwr., Wojciech M. Budzianowski
Wojciech Budzianowski
No abstract provided.
Mechanika Płynów Lab., Wojciech M. Budzianowski
Mechanika Płynów Lab., Wojciech M. Budzianowski
Wojciech Budzianowski
No abstract provided.
Enzyme Entrapment In Polyaniline Films Observed Via Florescence Anisotropy And Antiquenching, Louis R. Nemzer, Marissa Mccaffrey, Arthur J. Epstein
Enzyme Entrapment In Polyaniline Films Observed Via Florescence Anisotropy And Antiquenching, Louis R. Nemzer, Marissa Mccaffrey, Arthur J. Epstein
Louis R Nemzer
The facile entrapment of oxidoreductase enzymes within polyaniline polymer films by inducing hydrophobic collapse using phosphate buffered saline (PBS) has been shown to be a cost-effective method for fabricating organic biosensors. Here, we use fluorescence anisotropy measurements to verify enzyme immobilization and subsequent electron donation to the polymer matrix, both prerequisites for an effective biosensor. Specifically, we measure a three order of magnitude decrease in the ratio of the fluorescence to rotational lifetimes. In addition, the observed fluorescence antiquenching supports the previously proposed model that the polymer chain assumes a severely coiled conformation when exposed to PBS. These results help …