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Full-Text Articles in Soil Science

Complete Genome Sequences Of Chop, Delrio, And Grandslam, Three Gordonia Phages Isolated From Soil In Central Arkansas, Heidi N. Mathes, Elijah I. Christenson, John H. Crum, Emme M. Edmondson, Kassidy E. Gray, Luke W. Lawson, Lauren E. Lee, Michael P. Lee, Jackson A. Lipscomb, Morgan E. Masengale, Hannah G. Matthews, Charles M. Mcclain 4th, Tuesday N. Melton, Trace H. Morrow, Alexis M. Perry, David R. Rainwater, Grace E. Renois, Maryann F. Rettig, Duncan C. Troup, Allie J. Wilson, Nathan Reyna, Ruth Plymale Apr 2023

Complete Genome Sequences Of Chop, Delrio, And Grandslam, Three Gordonia Phages Isolated From Soil In Central Arkansas, Heidi N. Mathes, Elijah I. Christenson, John H. Crum, Emme M. Edmondson, Kassidy E. Gray, Luke W. Lawson, Lauren E. Lee, Michael P. Lee, Jackson A. Lipscomb, Morgan E. Masengale, Hannah G. Matthews, Charles M. Mcclain 4th, Tuesday N. Melton, Trace H. Morrow, Alexis M. Perry, David R. Rainwater, Grace E. Renois, Maryann F. Rettig, Duncan C. Troup, Allie J. Wilson, Nathan Reyna, Ruth Plymale

Articles

Chop, DelRio, and GrandSlam are phage with a Siphoviridae morphotype isolated from soil in Arkansas using the host Gordonia terrae 3612. All three are temperate, and their genomes share at least 96% nucleotide identity. These phage are assigned to cluster DI based on gene content similarity to other sequenced actinobacteriophage.


Soils Of The Central Nebraska Loess Hills And Central Loess Plains, Judith Turk, Rebecca Young, Nicolas A. Jelinsky, Amber D. Anderson, Ashlee Dere, Colby J. Moorberg, Rachel K. Owen Oct 2022

Soils Of The Central Nebraska Loess Hills And Central Loess Plains, Judith Turk, Rebecca Young, Nicolas A. Jelinsky, Amber D. Anderson, Ashlee Dere, Colby J. Moorberg, Rachel K. Owen

Conservation and Survey Division

Understanding soil systems that characterize a region is critical to natural resource management. However, the knowledge gained through intensive study of local soil systems, which takes place annually as part of collegiate soil judging contests, is often poorly preserved for future use. In this study, field descriptions and laboratory data for 16 soil profiles described for the 2019 Region 5 Soil Judging Contest were used to characterize the soil system of the Central Nebraska Loess Hills and Central Loess Plains. Three landscape components of this soil system were analyzed: the loess uplands and rainwater basins, the transitional zone, and bottomlands. …


Trace Element Contamination In Urban Soils: Testing And Management, Melissa Chilinski, Melanie Stock, Paul R. Grossl, Eli Oliver Apr 2022

Trace Element Contamination In Urban Soils: Testing And Management, Melissa Chilinski, Melanie Stock, Paul R. Grossl, Eli Oliver

All Current Publications

Trace elements, often referred to as heavy metals, naturally occur in the soil at low levels. Certain land use histories can elevate the concentrations of trace elements to levels that present health risks. Understanding which elements and soil test values may impact human or crop health is an important aspect of gardening and micro-farming, particularly in urban environments that are at increased risk of soil contamination. This fact sheet provides instructions on interpreting soil test results for trace elements through the Total Element Composition EPA 3050B Soil Test (#S19) at Utah State University Analytical Laboratory.


Biochar: Properties And Potential Benefits For Agricultural Soil In Rwanda, Andromede Uwase Jan 2022

Biochar: Properties And Potential Benefits For Agricultural Soil In Rwanda, Andromede Uwase

Honors Theses

Physical and chemical soil degradation is becoming a major challenge for agricultural productivity in Rwanda, which is the most important part of the country’s economy. The wide spreading soil degradation in Rwanda is mainly a result of naturally poor soils coupled with unsustainable soil management leading to, for example, accelerated soil erosion, acidification, nutrient loss, compaction, and to decreasing yields. Biochar, as an end product of pyrolysis of biomass in the absence of oxygen, has been proposed as a soil amendment in remediation strategies because of its positive effects on soil productivity relevant parameters such as soil pH, structure, nutrient …


A Foray Into Laboratory Scale Soil Incubations With Corn Stover And High Lignin Fermentation Byproduct, Michelle Wang Apr 2021

A Foray Into Laboratory Scale Soil Incubations With Corn Stover And High Lignin Fermentation Byproduct, Michelle Wang

ENGS 88 Honors Thesis (AB Students)

As the production of biofuels increases to meet the demands of a growing low carbon economy, questions of sustainability surrounding its feedstock and waste streams have become increasingly relevant. In the biofuel production process, crop residues like corn stover are harvested from the field and converted to biofuels leaving generating a residue called high lignin fermentation byproduct (HLFB). From extensive process modelling in the literature, it is suggested that HLFB should be either combusted to fuel auxiliary conversion processes or returned to the soil in place of the crop residues that were harvested. Currently, there is little literature testing the …


Determination Of Photon Shielding Parameters For Soils In Mangrove Forests, Frederick C. Hila, Gerald P. Dicen, Abigaile Mia V. Javier-Hila, Alvie Asuncion-Astronomo, Neil Raymund D. Guillermo, Roland V. Rallos, Ian A. Navarrete, Alberto V. Amorsolo Jr Feb 2021

Determination Of Photon Shielding Parameters For Soils In Mangrove Forests, Frederick C. Hila, Gerald P. Dicen, Abigaile Mia V. Javier-Hila, Alvie Asuncion-Astronomo, Neil Raymund D. Guillermo, Roland V. Rallos, Ian A. Navarrete, Alberto V. Amorsolo Jr

Environmental Science Faculty Publications

The mass attenuation coefficient (MAC), effective atomic number (Zeff), and effective electron density (Neff) values of soils in mangrove forests across the Philippine islands were investigated. In addition, ENDF/B-VI.8 cross-section data library was used for interpolating Zeff and Neff values. Photon energies considered in this study ranged from 59.5–1332 keV. MAC values were obtained using MCNP5 and PHITS Monte Carlo (MC) simulations and were found to be in good agreement with XCOM and values in literature. Results for Zeff were in good agreement with values obtained using Phy-X/ZeXTRa program. Each shielding parameter was …


A Survey On Subsurface Signal Propagation, Usman Raza, Abdul Salam Dec 2020

A Survey On Subsurface Signal Propagation, Usman Raza, Abdul Salam

Faculty Publications

Wireless Underground Communication (WUC) is an emerging field that is being developed continuously. It provides secure mechanism of deploying nodes underground which shields them from any outside temperament or harsh weather conditions. This paper works towards introducing WUC and give a detail overview of WUC. It discusses system architecture of WUC along with the anatomy of the underground sensor motes deployed in WUC systems. It also compares Over-the-Air and Underground and highlights the major differences between the both type of channels. Since, UG communication is an evolving field, this paper also presents the evolution of the field along with the …


Decision Agriculture, Abdul Salam, Usman Raza Aug 2020

Decision Agriculture, Abdul Salam, Usman Raza

Faculty Publications

In this chapter, the latest developments in the field of decision agriculture are discussed. The practice of management zones in digital agriculture is described for efficient and smart faming. Accordingly, the methodology for delineating management zones is presented. Modeling of decision support systems is explained along with discussion of the issues and challenges in this area. Moreover, the precision agriculture technology is also considered. Moreover, the chapter surveys the state of the decision agriculture technologies in the countries such as Bulgaria, Denmark, France, Israel, Malaysia, Pakistan, United Kingdom, Ukraine, and Sweden. Finally, different field factors such as GPS accuracy and …


Electromagnetic Characteristics Of The Soil, Abdul Salam, Usman Raza Aug 2020

Electromagnetic Characteristics Of The Soil, Abdul Salam, Usman Raza

Faculty Publications

The electromagnetic characteristics of the soil are discussed in this chapter. The characteristics of porous bedrock, soil medium, and impacts of rain attenuations are also presented. The models of dielectric soil properties are studied with a rigorous focus on the constitutive parameters of subsurface soil medium. Moreover, the permittivity and wavenumber in soil are explained. In addition, the frequency-dependent dielectric properties such as dispersion in soil, absorption characteristic, and penetration depth versus frequency are reviewed. Furthermore, the effective permittivity of soil–water mixture for through-the soil-propagation mechanism is analyzed thoroughly.


Underground Phased Arrays And Beamforming Applications, Abdul Salam, Usman Raza Aug 2020

Underground Phased Arrays And Beamforming Applications, Abdul Salam, Usman Raza

Faculty Publications

This chapter presents a framework for adaptive beamforming in underground communication. The wireless propagation is thoroughly analyzed to develop a model using the soil moisture as an input parameter to provide feedback mechanism while enhancing the system performance. The working of array element in the soil is analyzed. Moreover, the effect of soil texture and soil moisture on the resonant frequency and return loss is studied in detail. The wave refraction from the soil–air interface highly degrades the performance of the system. Furthermore, to beam steering is done to achieve high gain for lateral component improving the UG communication. The …


Signals In The Soil: Underground Antennas, Abdul Salam, Usman Raza Aug 2020

Signals In The Soil: Underground Antennas, Abdul Salam, Usman Raza

Faculty Publications

Antenna is a major design component of Internet of Underground Things (IOUT) communication system. The use of antenna, in IOUT, differs from traditional communication in that it is buried in the soil. Therefore, one of the main challenges, in IOUT applications, is to establish a reliable communication. To that end, there is a need of designing an underground-specific antenna. Three major factors that can impact the performance of a buried antenna are: (1) effect of high soil permittivity changes the wavelength of EM waves, (2) variations in soil moisture with time affecting the permittivity of the soil, and (3) difference …


Soil Moisture And Permittivity Estimation, Abdul Salam, Usman Raza Aug 2020

Soil Moisture And Permittivity Estimation, Abdul Salam, Usman Raza

Faculty Publications

The soil moisture and permittivity estimation is vital for the success of the variable rate approaches in the field of the decision agriculture. In this chapter, the development of a novel permittivity estimation and soil moisture sensing approach is presented. The empirical setup and experimental methodology for the power delay measurements used in model are introduced. Moreover, the performance analysis is explained that includes the model validation and error analysis. The transfer functions are reported as well for soil moisture and permittivity estimation. Furthermore, the potential applications of the developed approach in different disciplines are also examined.


Current Advances In Internet Of Underground Things, Abdul Salam, Usman Raza Aug 2020

Current Advances In Internet Of Underground Things, Abdul Salam, Usman Raza

Faculty Publications

The latest developments in Internet of Underground Things are covered in this chapter. First, the IOUT Architecture is discussed followed by the explanation of the challenges being faced in this paradigm. Moreover, a comprehensive coverage of the different IOUT components is presented that includes communications, sensing, and system integration with the cloud. An in-depth coverage of the applications of the IOUT in various disciplines is also surveyed. These applications include areas such as decision agriculture, pipeline monitoring, border control, and oil wells.


Signals In The Soil: Subsurface Sensing, Abdul Salam, Usman Raza Aug 2020

Signals In The Soil: Subsurface Sensing, Abdul Salam, Usman Raza

Faculty Publications

In this chapter, novel subsurface soil sensing approaches are presented for monitoring and real-time decision support system applications. The methods, materials, and operational feasibility aspects of soil sensors are explored. The soil sensing techniques covered in this chapter include aerial sensing, in-situ, proximal sensing, and remote sensing. The underlying mechanism used for sensing is also examined as well. The sensor selection and calibration techniques are described in detail. The chapter concludes with discussion of soil sensing challenges.


Autonomous Irrigation Management In Decision Agriculture, Abdul Salam, Usman Raza Aug 2020

Autonomous Irrigation Management In Decision Agriculture, Abdul Salam, Usman Raza

Faculty Publications

In this chapter, the important application of autonomous irrigation management in the field decision agriculture is discussed. The different types of sensor-guided irrigation systems are presented that includes center pivot systems and drip irrigation systems. Their sensing and actuator components are with detailed focus on real-time decision-making and integration to the cloud. This chapter also presents irrigation control systems which takes, as an input, soil moisture and temperature from IOUT and weather data from Internet and communicate with center pivot based irrigation systems. Moreover, the system architecture is explored where development of the nodes including sensing and actuators is presented. …


Variable Rate Applications In Decision Agriculture, Abdul Salam, Usman Raza Aug 2020

Variable Rate Applications In Decision Agriculture, Abdul Salam, Usman Raza

Faculty Publications

In this chapter, the variable rate applications (VRA) are presented for the field of decision agriculture. The characteristics of VRA control systems are described along with control hardware. Different types of VRA systems are discussed (e.g., liquid VRA systems and dry VRA systems). A case study is also explored in this regard. Moreover, recent advances and future trends are also outlined. Accordingly, a sustainable variable-rate irrigation scheduling is studied where different hardware and software component of the cyber-physical system are considered. Finally, chapter is concluded with a novel sensor deployment methodology.


The Post-Wildfire Impact Of Burn Severity And Age On Black Carbon Snow Deposition And Implications For Snow Water Resources, Cascade Range, Washington, Ted M. Uecker, Susan D. Kaspari, Keith N. Musselman, S. Mckenzie Skiles Aug 2020

The Post-Wildfire Impact Of Burn Severity And Age On Black Carbon Snow Deposition And Implications For Snow Water Resources, Cascade Range, Washington, Ted M. Uecker, Susan D. Kaspari, Keith N. Musselman, S. Mckenzie Skiles

Geological Sciences Faculty Scholarship

Wildfires in the snow zone affect ablation by removing forest canopy, which enhances surface solar irradiance, and depositing light absorbing particles [LAPs, such as black carbon (BC)] on the snowpack, reducing snow albedo. How variations in BC deposition affects post-wildfire snowmelt timing is poorly known and highly relevant to water resources. We present a field-based analysis of BC variability across five sites of varying burn age and burn severity in the Cascade Range, Washington State, United States. Single particle soot photometer (SP2) analyses of BC snow concentrations were used to assess the impact of BC on snow albedo, and radiative …


Empirical Models For Predicting Water And Heat Flow Properties Of Permafrost Soils, Michael T. O'Connor, M. Bayani Cardenas, Stephen B. Ferencz, Yue Wu, Bethany T. Neilson, Jingyi Chen, George W. Kling May 2020

Empirical Models For Predicting Water And Heat Flow Properties Of Permafrost Soils, Michael T. O'Connor, M. Bayani Cardenas, Stephen B. Ferencz, Yue Wu, Bethany T. Neilson, Jingyi Chen, George W. Kling

Publications

Warming and thawing in the Arctic are promoting biogeochemical processing and hydrologic transport in carbon‐rich permafrost and soils that transfer carbon to surface waters or the atmosphere. Hydrologic and biogeochemical impacts of thawing are challenging to predict with sparse information on arctic soil hydraulic and thermal properties. We developed empirical and statistical models of soil properties for three main strata in the shallow, seasonally thawed soils above permafrost in a study area of ~7,500 km2 in Alaska. The models show that soil vertical stratification and hydraulic properties are predictable based on vegetation cover and slope. We also show that …


Wayne E. Sabbe Arkansas Soil Fertility Studies 2019, Nathan A. Slaton May 2020

Wayne E. Sabbe Arkansas Soil Fertility Studies 2019, Nathan A. Slaton

Arkansas Agricultural Experiment Station Research Series

Rapid technological changes in crop management and production require that the research efforts be presented in an expeditious manner. The contributions of soil fertility and fertilizers are major production factors in all Arkansas crops. The studies described within will allow producers to compare their practices with the university’s research efforts. Additionally, soil-test data and fertilizer sales are presented to allow comparisons among years, crops, and other areas within Arkansas.


Soil Net Nitrogen Mineralisation Across Global Grasslands, A. C. Risch, S. Zimmerman, R. Ochoa-Hueso, M. Schütz, B. Frey, J. L. Firn, P. A. Fay, F. Hagedorn, E. T. Borer, E. W. Seabloom, W. S. Harpole, J. M. H. Knops, Rebecca L. Mcculley, A. A. D. Broadbent, C. J. Stevens, M. L. Silveria, P. B. Adler, S. Báez, L. A. Biederman, J. M. Blair Oct 2019

Soil Net Nitrogen Mineralisation Across Global Grasslands, A. C. Risch, S. Zimmerman, R. Ochoa-Hueso, M. Schütz, B. Frey, J. L. Firn, P. A. Fay, F. Hagedorn, E. T. Borer, E. W. Seabloom, W. S. Harpole, J. M. H. Knops, Rebecca L. Mcculley, A. A. D. Broadbent, C. J. Stevens, M. L. Silveria, P. B. Adler, S. Báez, L. A. Biederman, J. M. Blair

Plant and Soil Sciences Faculty Publications

Soil nitrogen mineralisation (Nmin), the conversion of organic into inorganic N, is important for productivity and nutrient cycling. The balance between mineralisation and immobilisation (net Nmin) varies with soil properties and climate. However, because most global-scale assessments of net Nmin are laboratory-based, its regulation under field-conditions and implications for real-world soil functioning remain uncertain. Here, we explore the drivers of realised (field) and potential (laboratory) soil net Nmin across 30 grasslands worldwide. We find that realised Nmin is largely explained by temperature of the wettest quarter, microbial biomass, clay content and bulk density. …


Carbon Dioxide Measurement In Irish Blanket Peatlands: An Assessment Of Pool-Soil Flux Variability, Mariya Radomski, Alan Gilmer, Vivienne Byers, Eugene Mcgovern Jan 2019

Carbon Dioxide Measurement In Irish Blanket Peatlands: An Assessment Of Pool-Soil Flux Variability, Mariya Radomski, Alan Gilmer, Vivienne Byers, Eugene Mcgovern

Articles

Peatlands have been recognised as having a significant role in the mediation of atmospheric carbon dioxide (CO2) levels with direct implications for global climate change. Longitudinal in situ measurement systems for CO2 concentrations in blanket peatland ecosystems are difficult to implement where the nature of terrestrial–aquatic connectivity and hydrodynamics have a significant effect on the carbon cycle. The need for greater data on CO2 concentrations and flux monitoring in these settings has been well recognised. However, applying the most appropriate monitoring approach presents a special challenge. This paper sets out the development of a direct method for field based longitudinal …


Managing Nitrous Oxide Emissions In Agricultural Fields, Mark S. Coyne, Wei Ren Sep 2017

Managing Nitrous Oxide Emissions In Agricultural Fields, Mark S. Coyne, Wei Ren

Plant and Soil Sciences Research Report

Agriculture is a major contributor to atmospheric nitrous oxide (N2O) (Smith et al., 2014; Tian et al., 2015). Unfortunately, nitrous oxide destroys stratospheric ozone (O3) which protects us from ultraviolet radiation (Cicerone, 1989) and it increases ground level O3, whichis an air pollutant threatening human health and food production. Nitrous oxide is also 298 times more potent than an equivalent amount of carbon dioxide (CO2) in terms of trapping and absorbing reflected solar radiation (Forster et al., 2007). Basic chemistry and physics assure us that increased levels of N2O in the …


Holdrege: Nebraska's State Soil, University Of Nebraska - Lincoln Aug 2017

Holdrege: Nebraska's State Soil, University Of Nebraska - Lincoln

Conservation and Survey Division

No abstract provided.


Holdrege Nebraska State Soil, Nebraska Society Of Professional Soil Scientists Aug 2017

Holdrege Nebraska State Soil, Nebraska Society Of Professional Soil Scientists

Conservation and Survey Division

No abstract provided.


Identifying And Addressing Soil Property Issues Affecting Roadside Vegetation Establishment, Xu Li, Martha Mamo, Walter H. Schacht, Tala Awada, Humberto Blanco-Canqui Aug 2016

Identifying And Addressing Soil Property Issues Affecting Roadside Vegetation Establishment, Xu Li, Martha Mamo, Walter H. Schacht, Tala Awada, Humberto Blanco-Canqui

Nebraska Department of Transportation: Research Reports

Attaining adequate vegetation cover along highways is important for NDOR to comply with EPA’s stormwater regulations. However, low plant cover is a common problem on shoulders (first 16 feet off the pavement) of many highways in Nebraska. The ultimate goal of this study is to identify cost-effective engineering solutions that assure adequate seed beds (i.e., soil conditions) for establishment of selected seeding mixtures. The objectives of this study are to (1) characterize the soil properties along roadsides where vegetation stands have not developed well, and (2) verify the effects of select soil property parameters on plant germination and establishment.

Sampling …


Timing Movement Of Water Through The Biosphere 2 Rainforest Using Deuterium As An Isotopic Tracer, Katie Almeda, Joost Van Haren, Till Volkmann Jan 2016

Timing Movement Of Water Through The Biosphere 2 Rainforest Using Deuterium As An Isotopic Tracer, Katie Almeda, Joost Van Haren, Till Volkmann

STAR Program Research Presentations

Rain forest ecosystems play an important role in global functions such as being home to half of the species of plants and animals on the planet, acting as a carbon sink, and moving water from the ground back into the atmosphere. In the Amazon, approximately 50 to 80% of moisture produced remains in the ecosystem’s water cycle. As a result of deforestation and rising global temperatures, less moisture can be moved back into the atmosphere. The objective of this study was to trace how water moves through a rain forest system in order to gain insight on how different species …


Phosphorus Release Potential Of Agricultural Soils Of The United States, Rebecca A. Young Dec 2015

Phosphorus Release Potential Of Agricultural Soils Of The United States, Rebecca A. Young

School of Natural Resources: Dissertations, Theses, and Student Research

Phosphorus (P) is one of the leading causes of surface water quality decline in the United States, leading to algal blooms and hypoxia in lakes and streams. Decreasing conservation funds dictate that agencies such as the Natural Resources Conservation Service, maximizes its effectiveness and efficiency in implementing practices to address P management and runoff on agricultural lands. Additional information on P behavior in soil is needed to improve P management plans to reduce pollution risk at the watershed, farm, and field scales. This research focuses on the development of total soil P release models, to be included into assessment and …


Rapid Response Tools And Datasets For Post-Fire Modeling: Linking Earth Observations And Process-Based Hydrological Models To Support Post-Fire Remediation, Mary Ellen Miller, Michael Billmire, William J. Elliot, K. A. Endsley, P. R. Robichaud May 2015

Rapid Response Tools And Datasets For Post-Fire Modeling: Linking Earth Observations And Process-Based Hydrological Models To Support Post-Fire Remediation, Mary Ellen Miller, Michael Billmire, William J. Elliot, K. A. Endsley, P. R. Robichaud

Michigan Tech Research Institute Publications

Preparation is key to utilizing Earth Observations and process-based models to support post-wildfire mitigation. Post-fire flooding and erosion can pose a serious threat to life, property and municipal water supplies. Increased runoff and sediment delivery due to the loss of surface cover and fire-induced changes in soil properties are of great concern. Remediation plans and treatments must be developed and implemented before the first major storms in order to be effective. One of the primary sources of information for making remediation decisions is a soil burn severity map derived from Earth Observation data (typically Landsat) that reflects fire induced changes …


Changes Of Soil Biogeochemistry Under Native And Exotic Plants Species, Yujie Hua Jan 2015

Changes Of Soil Biogeochemistry Under Native And Exotic Plants Species, Yujie Hua

FIU Electronic Theses and Dissertations

Invasive plant species are major threats to the biodiversity and ecosystem stability. The purpose of this study is to understand the impacts of invasive plants on soil nutrient cycling and ecological functions. Soil samples were collected from rhizosphere and non-rhizosphere of both native and exotic plants from three genera, Lantana, Ficus and Schinus, at Tree Tops Park in South Florida, USA. Experimental results showed that the cultivable bacterial population in the soil under Brazilian pepper (invasive Schinus) was approximately ten times greater than all other plants. Also, Brazilian pepper lived under conditions of significantly lower available phosphorus …


Quantitative Field Testing Heterodera Glycines From Metagenomic Dna Samples Isolated Directly From Soil Under Agronomic Production., Yan Li, Gary W. Lawrence, Shien Lu, Clarissa Balbalian, Vincent P. Klink Feb 2014

Quantitative Field Testing Heterodera Glycines From Metagenomic Dna Samples Isolated Directly From Soil Under Agronomic Production., Yan Li, Gary W. Lawrence, Shien Lu, Clarissa Balbalian, Vincent P. Klink

College of Agriculture & Life Sciences Publications and Scholarship

A quantitative PCR procedure targeting the Heterodera glycines ortholog of the Caenorhabditis elegans uncoordinated-78 gene was developed. The procedure estimated the quantity of H. glycines from metagenomic DNA samples isolated directly from field soil under agronomic production. The estimation of H. glycines quantity was determined in soil samples having other soil dwelling plant parasitic nematodes including Hoplolaimus, predatory nematodes including Mononchus, free-living nematodes and biomass. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from field soil.