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Full-Text Articles in Materials Chemistry
Direct Electrochemistry Of Glucose Oxidase Based On Ws2 Quantum Dots And Its Biosensing Application, Chen-Lu Li, Hua-Ping Peng, Zhong-Nan Huang, Yi-Lun Sheng, Pei-Wen Wu, Xin-Hua Lin
Direct Electrochemistry Of Glucose Oxidase Based On Ws2 Quantum Dots And Its Biosensing Application, Chen-Lu Li, Hua-Ping Peng, Zhong-Nan Huang, Yi-Lun Sheng, Pei-Wen Wu, Xin-Hua Lin
Journal of Electrochemistry
In this study, a novel electrochemical glucose biosensor has been developed by immobilizing glucose oxidase (GOx) on tungsten disulfide quantum dots (WS2 QDs) on the surface of glassy carbon electrode (GCE). Transmission electron microscopy, UV-vis spectroscopy and cyclic voltammetry were employed to characterize the morphology, structure, and electrochemical behaviors of the as-prepared WS2 QDs and the biofilm modified electrode. The results suggested that the WS2 QDs could accelerate the electron transfer between the electrode and the immobilized enzyme, which enabled the direct electrochemistry of GOx without any electron mediator. Besides, the immobilized GOx in WS2 QDsfilm …
Direct Electrochemistry And Glucose Biosensing Of Glucose Oxidase-Gold Nanoparticles Composite Synthesized By Enzyme Method, Fang He, Xiao-Li Qin, Ying-Chun Fu, Chao Chen, Qing-Ji Xie, Shou-Zhuo Yao
Direct Electrochemistry And Glucose Biosensing Of Glucose Oxidase-Gold Nanoparticles Composite Synthesized By Enzyme Method, Fang He, Xiao-Li Qin, Ying-Chun Fu, Chao Chen, Qing-Ji Xie, Shou-Zhuo Yao
Journal of Electrochemistry
Glucose oxidase (GOx)-gold nanoparticles (AuNPs) composite was synthesized by the one-pot enzyme reaction in the mixture solution of NaAuCl4, GOx and glucose, and a Nafion/GOx-AuNPs/glassy carbon electrode was fabricated by the cast-coating method to examine the direct electrochemistry of GOx and the biosensing performance on this electrode. Such a GOx-AuNPs composite showed good direct-electrochemistry activity and bioactivity of GOx, probably because the enzyme-mediated AuNPs are close to the redox active centers of GOx. This enzyme electrode exhibited a linear amperometric response to glucose concentration (0.54 mmol·L-1) at -0.4 V (vs. SCE), and a detection limit of …
Electrocatalytic Activities Of Mb/Aunps/Mwnts/Gc Electrode For Hydrogen Peroxide Reduction, Zheng-Juan Huang, Hua-Ping Peng, Dai-Jun Cha, Ai-Lin Liu, Wei Chen, Xin-Hua Lin, Yong-Ji You
Electrocatalytic Activities Of Mb/Aunps/Mwnts/Gc Electrode For Hydrogen Peroxide Reduction, Zheng-Juan Huang, Hua-Ping Peng, Dai-Jun Cha, Ai-Lin Liu, Wei Chen, Xin-Hua Lin, Yong-Ji You
Journal of Electrochemistry
A novel matrix, multiwalled carbon nanotubes supported Au nanoparticles composite nanomaterial (AuNPs/MWNTs), for immobilization of protein and biosensing was designed using a simple and effective one-step in situ synthesis route. Using myoglobin (Mb) as a model, the direct electrochemistry of the immobilized proteins on the AuNPs/MWNTs composite was studied. The results showed that the AuNPs/MWNTs composite can maintain the bioactivity and facilitate the direct electrochemistry of Mb in the Mb/AuNPs/MWNTs/GC electrode. Based on the direct electron transfer of the immobilized Mb, the protein electrode exhibited excellent electrocatalytic activity to the reduction of H2O2 with a linear range …
The Electrochemical Characteristics Of Cpo-Pll/Gc Electrode, Wen Zhao, Xia-Qin Wu, Zhong-Qing Lu, Wen-Jing Hou, He-Xing Li
The Electrochemical Characteristics Of Cpo-Pll/Gc Electrode, Wen Zhao, Xia-Qin Wu, Zhong-Qing Lu, Wen-Jing Hou, He-Xing Li
Journal of Electrochemistry
Poly-L-lysine(PLL) was electropolymerized onto the surface of glassy carbon(GC) electrode.1[(3-dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride(EDC) was used as a Cross-linking agent to immobilized Chloroperoxidase(CPO) onto the PLL/GC electrode.A pair of well-defined redox peaks was observed at CPO-PLL/GC electrode,which clearly suggested that PLL can promote the direct electron transfer between the CPO and the GC electrodes.The cyclic voltammetry results also showed that the electron transfer at CPO-PLL/GC is a surface adsorption-controlled quasi-reversible process,which is accompanied with proton transfer.The CPO-PLL/GC electrode exhibited a good electrochemical stability and excellently electrocatalytic activity for electrochemical reduction of O2.
Electrocatalysis Of Myoglobin-Chitosan-Au Colloid Film Modified Glass Carbon Electrode, Yu-Hua Qin, Jun Guo, Hui Xu, Li-Heng Zhou, Sheng-Tian Wang
Electrocatalysis Of Myoglobin-Chitosan-Au Colloid Film Modified Glass Carbon Electrode, Yu-Hua Qin, Jun Guo, Hui Xu, Li-Heng Zhou, Sheng-Tian Wang
Journal of Electrochemistry
The cyclic voltammgram of Mb/AAO/Au Colloid/GCE shows a pair of well-defined and nearly reversible peaks at about-0.20 V(vs.Ag/AgCl) in 0.1 mol/L NaAc-HAc buffer(pH = 5.4),being the characteristic peaks of the Mb heme Fe(Ⅲ) / Fe(Ⅱ)redox couples.The electron transfer between Mb and the electrode was greatly enhanced under present testing conditions.The effects of scan rate,pH and buffers concentration on the electrochemical behaviors of Mb were studied in detail.Position of the Soret absorbance band suggested that Mb in chitosan-colloidal gold hept its secondary structure similar to the native states.Oxygen was catalytically reduced by Mb in chitosan-colloidal gold films.
Fabrication Of Polyvinyl Alcohol Arrays And The Direct Electrochemistry Of Adsorbed Cytochrome C, Yu-Hua Qin, Ruo-Kun Jia, Qing-Juan Wei, Li Ma, Xiao-Hui Guan, Nan Lv
Fabrication Of Polyvinyl Alcohol Arrays And The Direct Electrochemistry Of Adsorbed Cytochrome C, Yu-Hua Qin, Ruo-Kun Jia, Qing-Juan Wei, Li Ma, Xiao-Hui Guan, Nan Lv
Journal of Electrochemistry
Regular polyvinyl alcohol arrays were prepared with porous polystyrene films by a self-assembly method. Cyt c immobilized on PVA microlens arrays modified glassy carbon electrodes gave a pair of stable,well-defined and quasi-reversible cyclic voltammetric peaks at about 0.072 V (vs. Ag/AgCl) in 0.1 mol/L PBS buffer (pH = 6.89) solution. The effects of scan rate,pH and concentration of buffers on the electrochemical behaviors of Cyt c were studied in detail. The apparent heterogeneous electron transfer rate constant (k0) was evaluated to be 2.98×10-6 cm·s-1.
Direct Electrochemistry Of Chloroperoxidase At Gc Electrode Modified With Swnts, Hong-Xia Zhang, Xia-Qin Wu, Wen-Jing Hou, Wen Xie, Zhong-Qing Lu, Rong Wang, He-Xing Li
Direct Electrochemistry Of Chloroperoxidase At Gc Electrode Modified With Swnts, Hong-Xia Zhang, Xia-Qin Wu, Wen-Jing Hou, Wen Xie, Zhong-Qing Lu, Rong Wang, He-Xing Li
Journal of Electrochemistry
The chloroperoxidase(CPO) was immobilized onto the surface of SWNTs modified electrodes.The electrochemical behaviors of modified electrode have been investigated by cyclic voltammetry.The appearance of a pair of well-defined redox peaks in the CV curve indicated that SWNTs can promote the electron transfer reaction of CPO.The results of cyclic voltammetry in PBS of different pH,from 3~9,showed that the reaction of CPO on the modified electrode was with equal numbers of electron and protein.This readily prepared SWNTs modified electrode showed excellent stability and good catalysis to O2.
Direct Electrochemistry Of God On Glassy Carbon Electrode Modified With Mesoporous Carbon, Kun-Qi Wang, Lin Zhu, Wei Xing
Direct Electrochemistry Of God On Glassy Carbon Electrode Modified With Mesoporous Carbon, Kun-Qi Wang, Lin Zhu, Wei Xing
Journal of Electrochemistry
Glucose oxidase(GOD) is immobilized on glassy carbon electrode mesoporous carbon by simple method.Cyclic voltammetric results indicated that on GOD of the modified electrode a quasi-reversible redox reaction took place(GCE) surface modified at a formal potential of-0.4294 V in 0.1 mol/L phosphate buffer solution(PBS)(pH 7.1).The electrochemical reaction consisted of a two-electron transfer coupled with a two-proton transfer.The GOD can completely catalyze oxidation of glucose via electron transfer intermedia of ferrocene monocarboxylic acid(FMCA) in saturated solutions with N2.The bioactivity of GOD on the modified electrode with mesoporous carbon was obviously unattacked.
Catalysis Of Nano Tio_2 For Horseradish Peroxidase, Ya-Qi Zhu, Hai Wu, Hiu Liu, Jie Ma
Catalysis Of Nano Tio_2 For Horseradish Peroxidase, Ya-Qi Zhu, Hai Wu, Hiu Liu, Jie Ma
Journal of Electrochemistry
Horseradish peroxidase immobilized on the surface of titanium dioxide nanoparticles and nanotube modified glassy carbon electrode.The HRP direct electron transfer reaction was compared between TiO2 nanoparticles and nanotube GC electrode.The results demonstrated that the HRP on TiO2 nanotube /GC electrode could catalyze electron exchange reaction between electroactivity center of HRP and electrode.And the research of antibody labeled HRP provides a selected signal for antibody-antigen immunoassay.
Immobilization Of Cytochrome C On The Surface Of Single-Wall Carbon Nanotube And Its Direct Electron Transfer And Electrocatalysis, Ya-Jing Yin, Ya-Fen Lv, Ping Wu, Pan Du, Yan-Mao Shi, Chen-Xin Cai
Immobilization Of Cytochrome C On The Surface Of Single-Wall Carbon Nanotube And Its Direct Electron Transfer And Electrocatalysis, Ya-Jing Yin, Ya-Fen Lv, Ping Wu, Pan Du, Yan-Mao Shi, Chen-Xin Cai
Journal of Electrochemistry
Cytochrome c(Cyto c) was immobilized on the surface of the single-wall carbon nanotube(SWNT) by using the method of adsorption.Infrared spectroscopy indicated that the Cyto c remained in its original structure and did not undergo structural change after its immobilization on the SWNT.The direct electrochemistry of cyto c,which was adsorbed on the surface of the SWNT,was studied by cyclic voltammetry.The voltammetric results demonstrated that the SWNT had promoting effects on the direct electron transfer of Cyto c and also indicated that the immobilized Cyto c retained its bioelectrocatalytic activity to the reduction of H_(2)O_(2).This modified electrode might be used in development …
Direct Electron Transfer Of Redox Proteins And Enzymes Promoted By Carbon Nanotube, Chen-Xin Cai
Direct Electron Transfer Of Redox Proteins And Enzymes Promoted By Carbon Nanotube, Chen-Xin Cai
Journal of Electrochemistry
The redox proteins (enzymes), such as hemoglobin (Hb), horseradish peroxidase (HRP) and glucose oxidase (GOx) were immobilized on the surface of the carbon nanotube modified with glassy carbon (CNT/GC) electrode, respectively. The experimental results showed that the redox proteins (enzymes) underwent effective and stable direct electron transfer reaction at the surface of CNT/GC electrode with a pair of nearly symmetrical redox peaks in phosphate buffer solution. The formal redox potential, E~(0'), is almost independent on the scan rates, the average value of E~(0') for Hb, HRP and GOx is -0.343±0.001, -0.319±0.002 and -0.456±0.001 V (vs.SCE,pH 6.9), respectively. The dependence of …