Inorganic Chemistry Commons^™

Spectroscopic Investigation Into The Heme Binding And The Heme Degradation By Mycobacterium Tuberculosis Mhud, Biswash Thakuri

Graduate College Dissertations and Theses

MhuD is a heme degrading enzyme found across mycobacteria. In the heme uptake machinery of Mycobacterium Tuberculosis, MhuD is a heme oxygenase (HO) involved in catalyzing the degradation of heme. Canonical HOs, such as human HO, have been shown to degrade heme to non-heme iron, carbon monoxide (CO) and biliverdin. However, MhuD has been demonstrated to degrade heme to non-heme iron and mycobilin without the release of CO. Formation of a novel chromophore by MhuD revealed that it follows a unique heme degradation mechanism compared to that of canonical HOs. Binding of heme to MhuD is the first step in …