Open Access. Powered by Scholars. Published by Universities.®
- Discipline
- Publication Type
Articles 1 - 2 of 2
Full-Text Articles in Analytical Chemistry
Electrochemical Biosensors For Wastewater-Based Epidemiology, Yu-Wei Pan, Kang Mao, Franziska Tuerk, Zhu-Gen Yang
Electrochemical Biosensors For Wastewater-Based Epidemiology, Yu-Wei Pan, Kang Mao, Franziska Tuerk, Zhu-Gen Yang
Journal of Electrochemistry
Wastewater-based epidemiology (WBE) has been shown to be an innovative approach for evaluation of drug use trends and public health assessment by quantifying drug residues and/or metabolites (so-called biomarkers) in wastewater collected in a local treatment plant. Community sewage sensors have been proposed and demonstrated to be powerful tools for the analysis of sewage biomarkers. In particular, electrochemical biosensors have emerged as a rapid method for the analysis of biomarkers and pathogens in wastewater due to low cost, minimal sample processing and the ability to test in the field. It has been widely used for biomedical diagnosis, environmental monitoring and …
Functionalized Aptamers For Detection Of Small-Molecule Targets, Haixiang Yu
Functionalized Aptamers For Detection Of Small-Molecule Targets, Haixiang Yu
FIU Electronic Theses and Dissertations
Aptamers have recently gained considerable attention for small-molecule detection in diverse applications such as drug identification, medical diagnostics, and environmental monitoring. However, the performance of aptamer-based sensors has been greatly limited by the low target affinity and responsiveness of small-molecule binding aptamers. This dissertation describes several novel aptamer engineering and isolation strategies to remedy this problem. Specifically, we first develop a generally applicable strategy to engineer split aptamers containing two binding domains termed cooperative-binding split aptamers (CBSAs). CBSAs exhibit higher target binding affinity and are far more responsive in terms of target-induced split aptamer assembly compared to single-domain parent split …