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Articles 1 - 4 of 4
Full-Text Articles in Analytical Chemistry
Functionalized Aptamers For Detection Of Small-Molecule Targets, Haixiang Yu
Functionalized Aptamers For Detection Of Small-Molecule Targets, Haixiang Yu
FIU Electronic Theses and Dissertations
Aptamers have recently gained considerable attention for small-molecule detection in diverse applications such as drug identification, medical diagnostics, and environmental monitoring. However, the performance of aptamer-based sensors has been greatly limited by the low target affinity and responsiveness of small-molecule binding aptamers. This dissertation describes several novel aptamer engineering and isolation strategies to remedy this problem. Specifically, we first develop a generally applicable strategy to engineer split aptamers containing two binding domains termed cooperative-binding split aptamers (CBSAs). CBSAs exhibit higher target binding affinity and are far more responsive in terms of target-induced split aptamer assembly compared to single-domain parent split …
Development Of Improved Extraction/Purification Methods And Comprehensive Screening/Confirmation By Lc-Qqq-Ms Analysis For Novel Psychoactive Substances, Ashley N. Kimble
Development Of Improved Extraction/Purification Methods And Comprehensive Screening/Confirmation By Lc-Qqq-Ms Analysis For Novel Psychoactive Substances, Ashley N. Kimble
FIU Electronic Theses and Dissertations
The presence of novel psychoactive substances (NPS) in forensic casework poses major difficulties for detection, since there are many structural variations of NPS circulating in the street market. There are currently no comprehensive screening/confirmatory/quantitation methods available that encompass the majority of NPS encountered in forensic toxicology. A major issue faced with developing such a method is that full validation is extremely time consuming. The use of a liquid chromatography triple quadrupole tandem mass spectrometry (LC-QqQ-MS/MS) method makes the detection of a large number of NPS possible because of high selectivity and sensitivity.
This research included four main tasks: 1) development …
Comprehensive Analysis Of Synthetic Cannabinoids And Metabolites In Oral Fluid By Liquid Chromatography Triple Quadrupole Mass Spectrometry, Carmen T. Mulet
Comprehensive Analysis Of Synthetic Cannabinoids And Metabolites In Oral Fluid By Liquid Chromatography Triple Quadrupole Mass Spectrometry, Carmen T. Mulet
FIU Electronic Theses and Dissertations
According to NIDA, synthetic cannabinoids (SC) include man-made, mind-altering chemicals. Their typically rapid metabolism and instability in biological matrices requires fast analytical methods for detection and confirmation; therefore, the use of oral fluid (OF) matrix is advantageous because collection is fast and non-invasive. The use of liquid chromatography triple quadrupole mass spectrometry (LC-QqQ-MS) and online solid phase extraction (SPE) for sample pretreatment minimizes bias and facilitates low level detection.
This study demonstrates the first use of online SPE for sample pretreatment in the comprehensive analysis of 100 SC and metabolites in OF by LC-QqQ-MS. The method was validated according the …
Interrogation Of Ion-Neutral Complexes By Trapped Ion Mobility-Mass Spectrometry And Theoretical Calculations, Alan A. Mckenzie Coe
Interrogation Of Ion-Neutral Complexes By Trapped Ion Mobility-Mass Spectrometry And Theoretical Calculations, Alan A. Mckenzie Coe
FIU Electronic Theses and Dissertations
In the present study, the fundamentals of molecular ion trapping and the application of trapped ion mobility spectrometry coupled to mass spectrometry (TIMS-MS) to the separation and identification of molecular components in complex mixtures are shown. In particular, ion-neutral complexes lifetimes, relative stability, binding energies and candidate structures are described for common explosives during TIMS-MS, as well as the effect of the micro-environment, during complex sample analysis. A novel TIMS-MS workflow was developed for the detection of both inorganic residues (IGSR; inorganic gunshot residues) and organic residues (OGSR) of firearm discharge residue from skin swabs, capable of high specificity and …