Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Materials Chemistry (3)
- Biochemistry, Biophysics, and Structural Biology (2)
- Life Sciences (2)
- Polymer Chemistry (2)
- Amino Acids, Peptides, and Proteins (1)
-
- Analytical, Diagnostic and Therapeutic Techniques and Equipment (1)
- Biochemistry (1)
- Biomedical and Dental Materials (1)
- Biophysics (1)
- Chemicals and Drugs (1)
- Investigative Techniques (1)
- Medicine and Health Sciences (1)
- Organic Chemistry (1)
- Other Analytical, Diagnostic and Therapeutic Techniques and Equipment (1)
- Other Biochemistry, Biophysics, and Structural Biology (1)
- Institution
- Keyword
-
- Mass spectrometry (2)
- Amphiphilic polymers (1)
- Antibody drug conjugates (1)
- Biomarkers (1)
- Cancer (1)
-
- Cetuximab-Staurosporine (1)
- Colloid (1)
- Complex coacervation (1)
- Covalent labeling (1)
- Drug to antibody ratio (1)
- Micelle (1)
- Phase separation (1)
- Positional isomer (1)
- Precipitation (1)
- Protein detection (1)
- Protein structural characterization (1)
- Proteins (1)
- Proteomics (1)
- Reverse micelles (1)
- Serum (1)
- Supramolecular assemblies (1)
Articles 1 - 5 of 5
Full-Text Articles in Analytical Chemistry
Characterization Of Β-2-Microglobulin Pre-Amyloid Oligomers And Their Role In Amyloid Inhibition, Tyler M. Marcinko
Characterization Of Β-2-Microglobulin Pre-Amyloid Oligomers And Their Role In Amyloid Inhibition, Tyler M. Marcinko
Doctoral Dissertations
In dialysis patients, β-2 microglobulin (β2m) can aggregate and eventually form amyloid fibrils in a condition known as dialysis-related amyloidosis, which deleteriously affects joint, bone, and organ function, and eventually causes organ failure. To understand the early stages of the amyloid assembly process, we have employed a series of biophysical tools including chromatography, spectroscopy, and most especially, native electrospray ionization (ESI) together with ion mobility mass spectrometry (IM-MS) to study soluble pre-amyloid oligomeric species. We have also collaborated and integrated computational modeling to help better understand and rationalize the structural basis behind oligomerization. Recently, several small molecules have been identified …
Protein Detection And Structural Characterization By Mass Spectrometry Using Supramolecular Assemblies And Small Molecules, Bo Zhao
Doctoral Dissertations
Mass spectrometry (MS) has played an increasingly prominent role in proteomics and structure biology because it shows superior capabilities in identification, quantification and structural characterization of proteins. To realize its full potential in protein analysis, significant progress has been made in developing innovative techniques and reagents that can couple to MS detection. This dissertation demonstrates the use of polymeric supramolecular assemblies for enhanced protein detection in complex biological mixtures by MS. An amphiphilic random co-polymer scaffold is developed to form functional supramolecular assemblies for protein/ peptide enrichment. The influences of charge density and functional group pKa on host-guest interactions …
Enhanced Mass Spectrometric Analysis Of Peptides And Proteins Using Polymeric Reverse Micelles, Mahalia Adelina Corazon Paningbatan Serrano
Enhanced Mass Spectrometric Analysis Of Peptides And Proteins Using Polymeric Reverse Micelles, Mahalia Adelina Corazon Paningbatan Serrano
Doctoral Dissertations
Mass spectrometry (MS) has become a key and indispensable tool in the identification, characterization, and quantitative analysis of proteins owing to its universality, sensitivity, specificity, and its capability for multiplexed detection. Because biological samples containing these protein analytes are almost always complex systems, various techniques are employed in conjunction with MS to fully harness its analytical potential and enhance its detection capabilities. This dissertation explores the use of amphiphilic polymeric reverse micelles in enriching proteins and peptides from complex biological mixtures and in enhancing their mass spectrometric analysis. Fundamental studies that elucidate the molecular basis for the observed MS signal …
Precipitation And Coacervation In Polyelectrolyte-Colloid Systems, Fatih Comert
Precipitation And Coacervation In Polyelectrolyte-Colloid Systems, Fatih Comert
Doctoral Dissertations
Complex coacervation between oppositely charged polyelectrolytes (PEs) and colloids (i.e. micelles and proteins) has increasingly become popular due to the use of these materials in a range of applications including but not limited to personal care, biomaterials, protein purification, and food science. In taking advantage of complex coacervation, which is a liquid-liquid phase separation, precipitation has always represented a problem by introducing inhomogeneities, irreversibility and irreproducible kinetics. Therefore, understanding the dominant factors driving the formation of precipitates is important to control outcomes. In this work, we have performed comparative studies of coacervation and precipitation in model PE-colloid systems to investigate …
Design, Synthesis, Characterization And Anti-Cancer Effects Evaluation Of Interchain Cysteine Linked Antibody Drug Conjugates, Ke Li
Doctoral Dissertations
"Antibody drug conjugates (ADCs) are emerging therapeutic products specially designed for the treatment of cancers. Potent cytotoxic agents are linked to antibodies via linkers. Antibody drug conjugates, which take advantage of both antibodies and cytotoxic agents, showed high selectivity towards cancer cells. Cytotoxic agents are delivered to the cancer cells selectively and kill them from inside while leaving low or non-toxicity towards normal cells. Antibody drug conjugates are actually heterogeneous mixtures. The major heterogeneities mainly lie in the drug to antibody ratio (DAR) and drug linking positions, both of which potentially affect the therapeutic index of ADCs. In this research, …