Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 4 of 4
Full-Text Articles in Analytical Chemistry
Fluorescence Quenching-Based Mechanism For Determination Of Hypochlorite By Coumarin-Derived Sensors, Karolina Starzak, Arkadiusz Matwijczuk, Bernadette Creaven, Alicja Matwijczuk, Sławomir Wybraniec, Dariusz Karcz
Fluorescence Quenching-Based Mechanism For Determination Of Hypochlorite By Coumarin-Derived Sensors, Karolina Starzak, Arkadiusz Matwijczuk, Bernadette Creaven, Alicja Matwijczuk, Sławomir Wybraniec, Dariusz Karcz
Articles
A fluorescence quenching-based mechanism for the determination of hypochlorite was proposed based on spectroscopic and chromatographic studies on the hypochlorite-sensing potency of three structurally similar and highly fluorescent coumarins. The mode of action was found to rely upon a chlorination of the coumarin-based probes resulting from their reaction with sodium hypochlorite. Importantly, the formation of chlorinated derivatives was accompanied by a linear decrease in the fluorescence intensities of the probes tested. The results obtained suggest the applicability of a coumarin-dependent hypochlorite recognition mechanism for the detection of, as well as for quantitative determination of, hypochlorite species in vitro.
Spheroid-3d And Monolayer-2d Intestinal Electrochemical Biosensor For Toxicity/Viability Testing: Applications In Drug Screening, Food Safety, And Environmental Pollutant Analysis, Evangelia Flampouri, Shahzad Imar, Kieran O'Connell, Baljit Singh
Spheroid-3d And Monolayer-2d Intestinal Electrochemical Biosensor For Toxicity/Viability Testing: Applications In Drug Screening, Food Safety, And Environmental Pollutant Analysis, Evangelia Flampouri, Shahzad Imar, Kieran O'Connell, Baljit Singh
Articles
The rise of three-dimensional cell culture systems that provide in vivo-like environments for pharmaco-toxicological models has prompted the need for simple and robust viability assays suitable for complex cell architectural structures. This study addresses that challenge with the development of an in vitro enzyme based electrochemical sensor for viability/cytotoxicity assessment of two-dimensional (2D) monolayer and three-dimensional (3D) spheroid culture formats. The biosensor measures the cell viability/toxicity via electrochemical monitoring of the enzymatic activity of nonspecific esterases of viable cells, through the hydrolysis of 1-naphthyl acetate to 1-naphthol. The proposed sensor demonstrated strong correlation (r = 0.979) with viable …
Rapid Analysis Of Magnesium In Infant Formula Powder Using Laser-Induced Breakdown Spectroscopy, Maria Markiewicz-Keszycka, Ming Zhao, Xavier Cama-Moncunill, Toufic El Arnaout, Dana Becker, Colm O'Donnell, P. J. Cullen, Carl Sullivan, Maria Piedad Casado-Gavalda
Rapid Analysis Of Magnesium In Infant Formula Powder Using Laser-Induced Breakdown Spectroscopy, Maria Markiewicz-Keszycka, Ming Zhao, Xavier Cama-Moncunill, Toufic El Arnaout, Dana Becker, Colm O'Donnell, P. J. Cullen, Carl Sullivan, Maria Piedad Casado-Gavalda
Articles
Laser-induced breakdown spectroscopy (LIBS) was investigated to determine magnesium (Mg) content in infant formula powder. To predict Mg content in the range established by the Codex Alimentarius, a partial least squares regression (PLSR) model was developed using a calibration data set (n = 30) based on full cross-validation and validated using an independent validation data set (n = 21). The prediction model performance was evaluated using the regression coefficients of determination (Rcv2 = 0.94 and Rp2 = 0.85) with the root mean square errors on cross-validation and prediction (RMSECV = 60 mg kg−1 and RMSEP = 80 mg kg−1). The …
Investigation Of Coastal Sea-Fog Formation Using The Wibs (Wide Band Integrated Bioaerosol Sensor) Technique, Shane M. Daly, David O'Connor, David A. Healy, Stig Hellebust, Jovanna Arndt, Eoinj. Mcgillicuddy, Patrick Feeney, Michael Quirke, John C. Wenger, John R. Sodeau
Investigation Of Coastal Sea-Fog Formation Using The Wibs (Wide Band Integrated Bioaerosol Sensor) Technique, Shane M. Daly, David O'Connor, David A. Healy, Stig Hellebust, Jovanna Arndt, Eoinj. Mcgillicuddy, Patrick Feeney, Michael Quirke, John C. Wenger, John R. Sodeau
Articles
Abstract. A wideband integrated bioaerosol sensor (WIBS4) was deployed in Haulbowline Island, Cork Harbour, to detect fluorescence particles in real time during July and September 2011. A scanning mobility particle sizer (SMPS) was also installed providing sizing analysis of the particles over the 10–450nm range. During the campaign, multiple fog formation events occurred; they coincided with dramatic increases in the recorded fluorescent particle counts. The WIBS sizing and fluorescence intensity profiles indicated that the origin of the signals was potentially non-biological in nature (i.e. PBAPs, primary biological aerosol particles). Furthermore, the data did not support the presence of known fluorescing …